CN102657071B - Method for culturing phytoplankton with predominant chlorophyta - Google Patents
Method for culturing phytoplankton with predominant chlorophyta Download PDFInfo
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- CN102657071B CN102657071B CN2012101722843A CN201210172284A CN102657071B CN 102657071 B CN102657071 B CN 102657071B CN 2012101722843 A CN2012101722843 A CN 2012101722843A CN 201210172284 A CN201210172284 A CN 201210172284A CN 102657071 B CN102657071 B CN 102657071B
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Abstract
The invention relates to a culture method for phytoplankton, belonging to the field of breeding. The invention discloses a method for culturing phytoplankton with predominant chlorophyta, comprising the following steps of: (1) producing culture solution, namely selecting cleaned aquatic plants with low cellulose content at first, and then adding a proper amount of water in the aquatic plants and placing the aquatic plants in a light-tight and warm environment to rot and ferment, and finally filtering the rotted and fermented solution of the aquatic plants to obtain the culture solution rich in nutrient elements such as nitrogen and phosphorus; (2) obtaining a natural eutrophic water body in late spring and early summer; (3) filtering large zooplankton and large individuals of phytoplankton in the obtained water body by a 200-mesh plankton mesh; (4) adding the culture solution produced in the step 1 in the water body filtered by the plankton mesh; and (5) culturing the culture water body with the culture solution in a transparent glass bottle at natural temperature under natural light, thus obtaining phytoplankton with predominant chlorophyta.
Description
Technical field
The present invention relates to the cultural method of a kind of phytoplankton, relate in particular to the method for cultivating the dominant phytoplankton of Chlorophyta.
Background technology
Chlorophyta and Cyanophyta phytoplankton all may become the sociales in the natural water body, and for cultivating pool, people more wish can occupy advantage to the useful Chlorophyta phytoplankton of the breed object in the cultivating pool in water body, therefore some have been arranged about the regulate and control method to phytoplankton dominant species in the natural water body, but existing these methods are more complicated all, at whole water body and its peripheral subenvironment, the regulation and control step that relates to is many, perhaps need the regulation and control of plant equipment assistance preferably, perhaps need to add the chemical reagent that may occur side effect to water body, and the culture fluid that seldom utilizes material to obtain easily carries out the phytoplankton cultivation, therefore cost is higher, even side effect may occur and polluted-water.
Summary of the invention
Technical problem to be solved by this invention provides a kind of method of cultivating the dominant phytoplankton of Chlorophyta, solves present regulate and control method cost height to phytoplankton dominant species in the natural water body, has the possible defective of side effect polluted-water.
Technical scheme
A kind of method of cultivating the dominant phytoplankton of Chlorophyta is characterized in that comprising the steps:
(1) makes culture fluid, step is: at first choose the low water plants of content of cellulose of clearing up totallyer, be positioned over putrid fermentation in the warm environment of black out after then water plants being added suitable quantity of water, the putrid fermentation liquid filtration with water plants at last obtains the abundant culture fluid of nutritive elements such as nitrogen, phosphorus;
Natural eutrophic water body when (2) obtaining the end of spring and the beginning of summer;
(3) utilize No. 25 plankton nets of 200 purposes with the macrozooplankton in the water body that obtains and big individual phytoplankton filtering;
(4) add the culture fluid of making in the step 1 in the water body behind No. 25 plankton net filtrations, and make and add that the net increase concentration of water body total nitrogen is about 5-8mg/L behind the culture fluid;
(5) be positioned in the Clear glass bottles and jars and cultivate adding cultivation water body behind the culture fluid, select to cultivate under natural temperature and the natural lighting condition, between culture period, timesharing every day repeatedly shakes up blake bottle, namely gets the dominant phytoplankton of Chlorophyta.
Further, add cultivation water body behind the culture fluid if incubation time is longer, as surpassing more than 20 days, repeat to add the culture fluid of making in the once described step 1 about then can be week about, make the net increase concentration of cultivating the water body total nitrogen is 2-5mg/L at every turn.
Further, make in the process of culture fluid in the described step 1, the water plants that the content of cellulose of choosing is low is mainly the phytoplankton of submerged plant, floatingleaved plant or high concentration.
Further, make in the process of culture fluid in the described step 1, during with the water plants putrid fermentation, as be aquatic macrophyte, then the ratio that plant weight in wet base and water are heavy is 1:2-4, if the wawter bloom that the Taihu Lake blue-green alga bloom then can directly utilize filter screen to fish for the water surface need not be added water again, make the chlorophyll a content of blue-green alga bloom greater than 1mg/L.
Further, make in the described step 1 in the process of culture fluid, during with the water plants putrid fermentation, the environment of fermentation by day the time maximum air temperature be preferably more than 20 degrees centigrade, fermentation time was preferably more than 1 month.
Further, make in the process of culture fluid in the described step 1, use 50 purpose filter screens with the bulky grain filtering in the zymotic fluid at last, obtain culture fluid.
Beneficial effect
The method of the dominant phytoplankton of cultivation Chlorophyta of the present invention promotes to limit the growth that the breed object is easy to generate the Cyanophyta plant of harm to culturing the growth of the useful Chlorophyta phytoplankton of object, and cultural method is simple; Drawing materials of use therein phytoplankton culture fluid obtains easily, and component is abundant, and macroelement and required other trace elements and the compositions such as required nitrogen, phosphorus of phytoplankton growth can be provided, and the preparation method is simple, is convenient to implement, and cost is low; Be particularly useful for testing the little water body cultivation of usefulness.
Description of drawings
Fig. 1 cultivates the phytoplankton that obtains for the embodiment of the invention 2 and amplifies 100 times schematic diagram at microscopically.
Fig. 2 cultivates the phytoplankton that obtains for the embodiment of the invention 2 and amplifies 400 times schematic diagram at microscopically.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.
Invent the method for a kind of simple dominant phytoplankton of cultivation Chlorophyta at hope, propose to utilize the mode of culture fluid, the preparation method of phytoplankton culture fluid is as follows:
1. choose the low water plants of content of cellulose of clearing up totallyer, be mainly the phytoplankton of submerged plant, floatingleaved plant or high concentration, as eel grass, black algae, waterweed, floating heart, duckweed, Taihu Lake blue-green alga bloom etc.;
2. be positioned over putrid fermentation in the warm environment of black out after water plants being added suitable quantity of water.If aquatic macrophytes such as eel grass, black algae, waterweed, floating heart, duckweeds, then the ratio that plant weight in wet base and water are heavy is about 1:3; If the wawter bloom that the Taihu Lake blue-green alga bloom then can directly utilize filter screen to fish for the water surface need not be added water again, make the chlorophyll a content of blue-green alga bloom greater than 1mg/L; The environment of fermentation by day the time maximum air temperature be preferably more than 20 degrees centigrade, fermentation time is more than 1 month;
3. the putrid fermentation liquid filtration with water plants obtains the abundant culture fluid of nutritive elements such as nitrogen, phosphorus.Can use 50 purpose filter screens with the bulky grain filtering in the zymotic fluid, namely obtain culture fluid.
The concrete grammar of cultivating the dominant phytoplankton of Chlorophyta is as follows:
1. the natural eutrophic water body when obtaining the end of spring and the beginning of summer;
2. utilize the 25# plankton net with the macrozooplankton in the water body that obtains and big individual phytoplankton filtering;
3. add above-mentioned said culture fluid among the present invention in the water body behind 25# plankton net filtration, and make and add that the net increase concentration of water body total nitrogen is about 5-8mg/L behind the culture fluid;
4. the cultivation water body behind the interpolation culture fluid is positioned in the Clear glass bottles and jars and cultivates, select to cultivate under natural temperature and the natural lighting condition.Between culture period, every morning, noon and dusk respectively shake up blake bottle one time, namely get the phytoplankton that Chlorophyta is advantage;
5. if incubation time is longer, as surpassing more than 20 days, repeat to add the culture fluid that said method obtains about then can be approximately week about, make the net increase concentration of water body total nitrogen be about 3mg/L at every turn.
The employing for preparing of culture fluid is carried out natural fermented method to water plants among the present invention, and do not need other consumed energy, and need not add chemical reagent, material environment friendly, process is simple; The ratio of macroelement content such as the nitrogen in the water plants body, phosphorus and the demand of Chlorophyta phytoplankton are more approaching, need not regulate and control the N/P ratio example; Contain the required trace element of phytoplankton growth and small-molecular peptides, sugar, vitamin etc. in the culture fluid of fermentation back, can satisfy the phytoplankton growth demand; Organic many in the culture fluid of fermentation back, zooplankton often breeds very slowly therein, thereby the biomass of zooplankton is little, thereby zooplankton is very little to the selectivity predator pressure of phytoplankton, is conducive to Chlorophyta phytoplankton acquisition advantage in the nutritive salt competition.
The concrete grammar step of the dominant phytoplankton of cultivation Chlorophyta among the present invention is simple.Select for use the time of natural eutrophic water body when the end of spring and the beginning of summer, the phytoplankton in this time often contains the Chlorophyta phytoplankton of larger proportion, can guarantee like this that wherein the Cyanophyta plant does not occupy advantage in the time of also can be as summer, and utilize the 25# plankton net that wherein macrozooplankton and big individual phytoplankton are carried out filtering, can reduce zooplankton like this to the selectivity predator pressure of phytoplankton, thereby be conducive to Chlorophyta plant acquisition advantage in competition wherein, wherein be specially which kind of Chlorophyta phytoplankton and occupy the dominant species that advantage then depends on phytoplankton in the selected initial water body.When if incubation time is longer, can satisfy the lasting growth of phytoplankton by continuing to add culture fluid.
In sum, the present invention compares to prior art and has following advantage: can promote to limit the growth that the breed object is easy to generate the Cyanophyta plant of harm to culturing the growth of the useful Chlorophyta phytoplankton of object, cultural method is simple; And drawing materials of the phytoplankton culture fluid of its use obtains easily, and component is abundant, and macroelement and required other trace elements and the compositions such as required nitrogen, phosphorus of phytoplankton growth can be provided, and the preparation method is simple, is convenient to implement, and cost is low; The little water body that is applicable to experiment usefulness is cultivated.
With 2 embodiment the present invention is described below, but therefore is not construed as limiting the invention.
Embodiment 1:
The method that the present embodiment explanation utilizes the rot of submerged plant eel grass to cultivate the dominant phytoplankton of Scenedesmus of Chlorophyta as culture fluid.
Obtain the submerged plant eel grass, clean the about 1.5kg of back weight in wet base, be positioned over that putrid fermentation used 50 purpose filter screens with the bulky grain filtering in the zymotic fluid more than 1 month in about 5kg water body, namely obtain culture fluid.
Obtain water body in the natural pond of the end of spring and the beginning of summer, utilize the 25# plankton net with the macrozooplankton in the water body that obtains and big individual phytoplankton filtering, find that at the microscopically microscopy phytoplankton sociales are the Scenedesmus of Chlorophyta in the water body of filter back.Filter back water body is placed in the colourless transparent glass bottle of 3 10L, and every bottle of water volume is 10L, as 3 parallel processing, adds above-mentioned culture fluid and cultivates, and make and add that the net increase concentration of water body nitrogen is about 6mg/L behind the culture fluid.Between culture period, every morning, noon and respectively shake up once each blake bottle at dusk, get final product the dominant phytoplankton of Scenedesmus of Chlorophyta.And the dominant situation of this Scenedesmus is can fine keeping about 3 week the time at incubation time.
Embodiment 2:
The method that the present embodiment explanation utilizes the rot of fluitante duckweed to cultivate the dominant phytoplankton of Ankistrodesmus of Chlorophyta as culture fluid.
Obtain the floatingleaved plant duckweed, clean the about 1kg of back weight in wet base, be positioned over that putrid fermentation used 50 purpose filter screens with the bulky grain filtering in the zymotic fluid more than 1 month in about 3kg water body, namely obtain culture fluid.
Obtain water body in the natural ornamental fishpond of the end of spring and the beginning of summer, utilize the 25# plankton net with the macrozooplankton in the water body that obtains and big individual phytoplankton filtering, find that at the microscopically microscopy phytoplankton sociales are the Ankistrodesmus of Chlorophyta in the water body of filter back.Filter back water body is placed in the colourless transparent glass bottle of 3 5L, and every bottle of water volume is 5L, as 3 parallel processing, adds above-mentioned culture fluid and cultivates, and make and add that the net increase concentration of water body nitrogen is about 6mg/L behind the culture fluid.Between culture period, every morning, noon and respectively shake up once each blake bottle at dusk, get final product the dominant phytoplankton of Ankistrodesmus of Chlorophyta.And the dominant situation of this Scenedesmus is can fine keeping about 3 week the time at incubation time.When incubation time is longer, by repeating to add above-mentioned duckweed the culture fluid of coming that rots, the time interval of adding was about for 1 week, made the net increase concentration of water body total nitrogen be about 3mg/L at every turn, still can obtain the dominant phytoplankton of Ankistrodesmus of Chlorophyta.
Attached Fig. 1 and 2 is respectively and cultivates the phytoplankton obtain and amplify the schematic diagram of 100 times and 400 times at microscopically, can see obviously that the Ankistrodesmus of Chlorophyta preponderates.
Claims (6)
1. a method of cultivating the dominant phytoplankton of Chlorophyta is characterized in that comprising the steps:
(1) makes culture fluid, step is: at first choose the low water plants of content of cellulose of clearing up totallyer, be positioned over putrid fermentation in the warm environment of black out after then water plants being added suitable quantity of water, the putrid fermentation liquid filtration with water plants at last obtains nitrogen, the abundant culture fluid of phosphorus nutrition element;
Natural eutrophic water body when (2) obtaining the end of spring and the beginning of summer;
(3) utilize No. 25 plankton nets of 200 purposes with the macrozooplankton in the water body that obtains and big individual phytoplankton filtering;
(4) add the culture fluid of making in the step 1 in the water body behind No. 25 plankton net filtrations, and make and add that the net increase concentration of water body total nitrogen is about 5-8mg/L behind the culture fluid;
(5) be positioned in the Clear glass bottles and jars and cultivate adding cultivation water body behind the culture fluid, select to cultivate under natural temperature and the natural lighting condition, between culture period, timesharing every day repeatedly shakes up blake bottle, namely gets the dominant phytoplankton of Chlorophyta.
2. the method for the dominant phytoplankton of cultivation Chlorophyta as claimed in claim 1, it is characterized in that: the cultivation water body behind the interpolation culture fluid is if incubation time is longer, repeat to add the culture fluid of making in the once described step 1 about then can be week about, make the net increase concentration of cultivating the water body total nitrogen is 2-5mg/L at every turn.
3. the method for the dominant phytoplankton of cultivation Chlorophyta as claimed in claim 1 or 2, it is characterized in that: make in the process of culture fluid in the described step 1, the water plants that the content of cellulose of choosing is low is mainly the phytoplankton of submerged plant, floatingleaved plant or high concentration.
4. the method for the dominant phytoplankton of cultivation Chlorophyta as claimed in claim 1 or 2, it is characterized in that: make in the described step 1 in the process of culture fluid, during with the water plants putrid fermentation, as be aquatic macrophyte, then the ratio that plant weight in wet base and water are heavy is 1:2-4, if the wawter bloom that the Taihu Lake blue-green alga bloom then can directly utilize filter screen to fish for the water surface need not be added water again, make the chlorophyll a content of blue-green alga bloom greater than 1mg/L.
5. the method for the dominant phytoplankton of cultivation Chlorophyta as claimed in claim 1 or 2, it is characterized in that: make in the described step 1 in the process of culture fluid, during with the water plants putrid fermentation, the environment of fermentation by day the time maximum air temperature be preferably more than 20 degrees centigrade, fermentation time was preferably more than 1 month.
6. the method for the dominant phytoplankton of cultivation Chlorophyta as claimed in claim 1 or 2 is characterized in that: make in the process of culture fluid in the described step 1, use 50 purpose filter screens with the bulky grain filtering in the zymotic fluid at last, obtain culture fluid.
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| CN103719024B (en) * | 2013-12-04 | 2015-09-23 | 刘军亮 | A kind ofly strengthen plankton and to ingest the microbial culture method of blue-green alge ability |
| CN104651234A (en) * | 2015-02-10 | 2015-05-27 | 中国水产科学研究院渔业机械仪器研究所 | Method for culturing high-density fresh water attached diatom |
| CN105112338B (en) * | 2015-09-17 | 2019-01-22 | 中国科学院南京地理与湖泊研究所 | A method of utilizing lake blue algae wawter bloom culture planktonic green algae |
| CN105600942B (en) * | 2015-09-24 | 2019-01-29 | 中国水产科学研究院渔业机械仪器研究所 | A method of biological flocculation is formed using cyanobacterial bloom |
| CN105994036B (en) * | 2016-05-24 | 2019-11-05 | 苑春亭 | A kind of grow to shellfish provides the fish-farming in shallow marine water method of food organisms |
| CN110255715B (en) * | 2019-06-20 | 2021-08-20 | 中国水产科学研究院渔业机械仪器研究所 | Method for cultivating diatom dominant freshwater biological flocs by using culture tail water |
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| CN1930949A (en) * | 2005-09-16 | 2007-03-21 | 中国科学院大连化学物理研究所 | Fast marine green algae cultivating process |
| CN101050419A (en) * | 2007-05-18 | 2007-10-10 | 王逢旦 | Production apparatus for culturing tiny alga and production method |
| CN101503264A (en) * | 2009-03-13 | 2009-08-12 | 上海大祺环保工程有限公司 | Method for ectopically and ecologically repairing eutrophication water |
| CN102154110A (en) * | 2011-01-27 | 2011-08-17 | 华东理工大学 | High-yield microalgae cultivating method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1930949A (en) * | 2005-09-16 | 2007-03-21 | 中国科学院大连化学物理研究所 | Fast marine green algae cultivating process |
| CN101050419A (en) * | 2007-05-18 | 2007-10-10 | 王逢旦 | Production apparatus for culturing tiny alga and production method |
| CN101503264A (en) * | 2009-03-13 | 2009-08-12 | 上海大祺环保工程有限公司 | Method for ectopically and ecologically repairing eutrophication water |
| CN102154110A (en) * | 2011-01-27 | 2011-08-17 | 华东理工大学 | High-yield microalgae cultivating method |
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