CN105925471A - High-density diatom culture dish and diatom culture method - Google Patents
High-density diatom culture dish and diatom culture method Download PDFInfo
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- CN105925471A CN105925471A CN201610335590.2A CN201610335590A CN105925471A CN 105925471 A CN105925471 A CN 105925471A CN 201610335590 A CN201610335590 A CN 201610335590A CN 105925471 A CN105925471 A CN 105925471A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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Abstract
The invention discloses a high-density diatom culture dish and a diatom culture method. The high-density diatom culture dish is characterized by comprising a culture container, a positioning core post, a round rod and a green LED (Light Emitting Diode) light source, wherein the positioning core post is of a transparent structure. The high-density diatom culture method comprises the steps of nutrient solution preparation, filtering treatment, insolation, heating and aeration. Through the arrangement of the positioning core post in the culture container, the green LED light source is wound on the round rod arranged in the positioning core post; the positioning core post is of the transparent structure, so that the green LED light source can penetrate through the positioning core post to be irradiated into a water body in the culture container; and under the irradiation of the green light, the content of chlorophyll a in diatom cells is the highest. The diatom colony general growth trends are of a weak light adapting type, a red light sensitive type and a blue light inhibiting type; the green light can efficiently activate chlorophyll molecules, so that the photosynthetic rate can be accelerated; and cell contents such as chlorophyll in the diatom cells can be improved, and the cell division can be accelerated.
Description
Technical field
The present invention relates to a kind of cultural method and cultivating apparatus, particularly relate to a kind of high density diatom culture dish and diatom cultural method.
Background technology
Freshwater Diatom is aquatic animal, nutritious foodstuff such as fish, shrimp, Eriocheir sinensis, freshwater mussel particularly its germling, both at home and abroad the work report in terms of the classification of relevant diatom, physiological ecological is more, and has the report that more sea water diatom mass propgation studies, but the research about Freshwater Diatom cultivation is less.This may compare abundant with diatom kind and Biomass in sea water, and in fresh water, diatom Biomass is relatively fewer relevant.Further, along with the universal eutrophication of poisons in freshwater, often there is blue-green alga bloom in poisons in freshwater, and diatom proportion is considerably less.And it is cladded with siliceous cell wall owing to of diatom is mainly characterized by frustule, therefore it is generally acknowledged that the growth of diatom is higher to the demand of element silicon concentration in water body.And with chlorella, cyanophyceae are compared, diatom is typically more easy to be attached to grown on matrix.Diatom is the most fewer as the research in terms of the microalgae energy.Diatom of many uses, except being widely used in addition in terms of aquatic feed, diatom is all widely used at aspects such as biological medicine, biological restoration, chemical materials, nanotechnologys, has the most wide research and application prospect.
Summary of the invention
The technical problem to be solved is to provide a kind of high density diatom culture dish and diatom cultural method, has the feature of applicable algae fast high-density growth.
For solving above-mentioned technical problem, technical scheme to be solved by this invention is: a kind of high density diatom culture dish, its innovative point is: described culture dish includes the culture vessel of upper shed, the location stem stem being arranged in described culture vessel, the pole being arranged in the stem stem of described location, the green LED light source that is wrapped on described pole, being provided with the hole, location passed for described pole on the stem stem of described location, described location stem stem is transparent configuration.
Preferably, described location stem stem is structure as a whole with described culture vessel.
Preferably, described culture vessel is transparent configuration.
Preferably, the end face of location stem stem is concordant with described culture vessel.
Preferably, the end face of described location stem stem is provided with the capping coordinated with described location stem stem.
Preferably, it is provided with sealing device between described capping and described location stem stem.
Preferably, at least provided with a described location stem stem in described culture vessel.
Preferably, it is provided with temperature sensor in described culture vessel.
Preferably, it is provided with heater in described culture vessel.
A kind of high density diatom cultural method, applies above-mentioned a kind of high density diatom culture dish, comprises the steps:
A: make nutritional solution, uses forage to mince process and throws in culture vessel, and then interpolation tap water in culture vessel, and forage is 1:1-2 with the ratio of tap water, then carry out putrid fermentation process, fermentation 3～6 months, with when wherein stink becomes thin；
B: nutritional solution is carried out filtration treatment, is blended into nitrogenous fertilizer in the nutritional solution after filtration treatment, nitrogenous fertilizer and nutritional solution ratio are 1:50-80, after interpolation, the instant concentration of water body total nitrogen content reaches 6-12mg/L, and the concentration of dissolved organic carbon DOC is at 7-9mg/L；
C: culture vessel is placed in glasshouse or outdoor have solar radiation place but need to when the rainy day rain cover, really
Guarantor's illumination condition is the intensity of solar radiation in winter, spring；
D: then heat the water body that with the addition of nutritional solution in culture vessel, maintains the water temperature of water body between 20-30 DEG C；
E: heating carries out aeration simultaneously to the water body in nutritional solution, aeration rate size, to maintain the dissolved oxygen saturated concentration of water body to be as the criterion between 60%-110%, makes water body swim the chlorophyll-a concentration of state at 60 below μ g/L；
F: aeration 10～after 14 days, the wall of culture vessel can grow speckle russet, be i.e. diatom.
It is an advantage of the current invention that: use the culture dish of said structure, by arranging location stem stem in culture vessel, being wound around green LED light source on pole in the stem stem of location, owing to location stem stem is transparent configuration, green LED light source can penetrate location stem stem and be irradiated in the water body in culture vessel.Light quality is used to describe the difference character of the illuminating effect that light is caused because of spectral composition difference.Light quality affects many aspects of growth of diatom algae, such as photosynthesis, chemical composition, growth rate, group, population dynamic and adaptive pattern etc..Different types of diatom is different to the sensitivity of light quality.Under the irradiation of green glow, the content of alga cells inner chlorophyll a is the highest.The overall growth of Diatomeae group is dynamically low light level epharmone, red light sensitiveness type and blue light suppressive, and green glow can efficiently activate chlorophyll molecule, thus can accelerate photosynthetic rate, and causes the raising of the cell inclusions such as alga cells inner chlorophyll and fissional quickening.
Accompanying drawing explanation
The present invention is described in further detail with detailed description of the invention below in conjunction with the accompanying drawings.
Fig. 1 is the perspective view one of the present invention.
Fig. 2 is the perspective view two of the present invention.
Fig. 3 is the cross-sectional view of the present invention.
In figure: 1-culture vessel, 2-location stem stem, 3-pole, 4-green LED light source, 5-position hole, 6-capping, 7-sealing device, 8-temperature sensor, 9-heater.
Detailed description of the invention
As shown in Figure 1 to Figure 3, the culture vessel 1 that the high density diatom culture dish of the present invention includes upper shed, the location stem stem 2 being arranged in culture vessel 1, the pole 3 being arranged in the stem stem 2 of location, the green LED light source 4 that is wrapped on pole 3, being provided with the hole, location 5 passed for pole 3 on the stem stem 2 of location, location stem stem 2 is transparent configuration.Use the culture dish of said structure, by arranging location stem stem 2 in culture vessel 1, being wound around green LED light source 4 on pole 3 in the stem stem 2 of location, owing to location stem stem 2 is transparent configuration, green LED light source 4 can penetrate location stem stem 2 and be irradiated in the water body in culture vessel 1.Light quality is used to describe the difference character of the illuminating effect that light is caused because of spectral composition difference.Light quality affects many aspects of growth of diatom algae, such as photosynthesis, chemical composition, growth rate, group, population dynamic and adaptive pattern etc..Different types of diatom is different to the sensitivity of light quality.Under the irradiation of green glow, the content of alga cells inner chlorophyll a is the highest.The overall growth of Diatomeae group is dynamically low light level epharmone, red light sensitiveness type and blue light suppressive, and green glow can efficiently activate chlorophyll molecule, thus can accelerate photosynthetic rate, and causes the raising of the cell inclusions such as alga cells inner chlorophyll and fissional quickening.
For the ease of Real Time Observation, culture vessel is transparent configuration.In order to ensure bonding strength and waterproof purpose, location stem stem 2 is structure as a whole with culture vessel 1.In order to ensure aesthetic feeling and be prone to processing, the end face of location stem stem 2 is concordant with culture vessel 1.In order to prevent water from entering in the stem stem 2 of location, the end face of location stem stem 2 it is provided with and positions the capping 6 that stem stem 2 coordinates, in order to play better seal effect, between capping 6 and location stem stem 2, being provided with sealing device 7.For the culture dish reasonable distribution green LED light source 4 for different volumes, at least provided with a location stem stem 2 in culture vessel 1.In order to monitor the temperature in culture dish in real time, in culture vessel 1, it is provided with temperature sensor 8, for the ease of improving the temperature of water body in culture dish, in culture vessel 1, is provided with heater 9.
The high density diatom cultural method that the present invention is corresponding, applies above-mentioned high density diatom culture dish, comprises the steps:
A: make nutritional solution, uses forage to mince process and throws in culture vessel, and then interpolation tap water in culture vessel, and forage is 1:1-2 with the ratio of tap water, then carry out putrid fermentation process, fermentation 3～6 months, with when wherein stink becomes thin；
B: nutritional solution is carried out filtration treatment, is blended into nitrogenous fertilizer in the nutritional solution after filtration treatment, nitrogenous fertilizer and nutritional solution ratio are 1:50-80, after interpolation, the instant concentration of water body total nitrogen content reaches 6-12mg/L, and the concentration of dissolved organic carbon DOC is at 7-9mg/L；
C: culture vessel is placed in glasshouse or outdoor have solar radiation place but need to when the rainy day rain cover, really
Guarantor's illumination condition is the intensity of solar radiation in winter, spring；
D: then heat the water body that with the addition of nutritional solution in culture vessel, maintains the water temperature of water body between 20-30 DEG C；
E: heating carries out aeration simultaneously to the water body in nutritional solution, aeration rate size, to maintain the dissolved oxygen saturated concentration of water body to be as the criterion between 60%-110%, makes water body swim the chlorophyll-a concentration of state at 60 below μ g/L；
F: aeration 10～after 14 days, the wall of culture vessel can grow speckle russet, be i.e. diatom.
Finally it should be noted that, above example is only in order to illustrate technical scheme and non-limiting technical scheme, it will be understood by those within the art that, technical scheme is modified or equivalent by those, without deviating from objective and the scope of the technical program, all should contain in the middle of scope of the presently claimed invention.
Claims (10)
1. a high density diatom culture dish, it is characterized in that: described culture dish includes the culture vessel of upper shed, the location stem stem being arranged in described culture vessel, the pole being arranged in the stem stem of described location, the green LED light source that is wrapped on described pole, being provided with the hole, location passed for described pole on the stem stem of described location, described location stem stem is transparent configuration.
2. a kind of high density diatom culture dish as claimed in claim 1, it is characterised in that: described location stem stem is structure as a whole with described culture vessel.
3. a kind of high density diatom culture dish as claimed in claim 1, it is characterised in that: described culture vessel is transparent configuration.
4. a kind of high density diatom culture dish as claimed in claim 1, it is characterised in that: the end face of location stem stem is concordant with described culture vessel.
5. a kind of high density diatom culture dish as claimed in claim 4, it is characterised in that: it is provided with the capping coordinated with described location stem stem on the end face of described location stem stem.
6. a kind of high density diatom culture dish as claimed in claim 5, it is characterised in that: it is provided with sealing device between described capping and described location stem stem.
7. a kind of high density diatom culture dish as claimed in claim 1, it is characterised in that: at least provided with a described location stem stem in described culture vessel.
8. a kind of high density diatom culture dish as claimed in claim 1, it is characterised in that: it is provided with temperature sensor in described culture vessel.
9. a kind of high density diatom culture dish as claimed in claim 1, it is characterised in that: it is provided with heater in described culture vessel.
10. a high density diatom cultural method, a kind of high density diatom culture dish that application claim 1 to 9 any of which one is described, it is characterised in that comprise the steps:
A: make nutritional solution, uses forage to mince process and throws in culture vessel, and then interpolation tap water in culture vessel, and forage is 1:1-2 with the ratio of tap water, then carry out putrid fermentation process, fermentation 3～6 months, with when wherein stink becomes thin；
B: nutritional solution is carried out filtration treatment, is blended into nitrogenous fertilizer in the nutritional solution after filtration treatment, nitrogenous fertilizer and nutritional solution ratio are 1:50-80, after interpolation, the instant concentration of water body total nitrogen content reaches 6-12mg/L, and the concentration of dissolved organic carbon DOC is at 7-9mg/L；
C: culture vessel is placed in glasshouse or outdoor have solar radiation place but need to when the rainy day rain cover, really
Guarantor's illumination condition is the intensity of solar radiation in winter, spring；
D: then heat the water body that with the addition of nutritional solution in culture vessel, maintains the water temperature of water body between 20-30 DEG C；
E: heating carries out aeration simultaneously to the water body in nutritional solution, aeration rate size, to maintain the dissolved oxygen saturated concentration of water body to be as the criterion between 60%-110%, makes water body swim the chlorophyll-a concentration of state at 60 below μ g/L；
F: aeration 10～after 14 days, the wall of culture vessel can grow speckle russet, be i.e. diatom.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110184168A (en) * | 2019-05-21 | 2019-08-30 | 荆楚理工学院 | A kind of simple astaxanthin incubator and the method using its culture astaxanthin |
| CN111423985A (en) * | 2020-04-29 | 2020-07-17 | 中国水产科学研究院渔业机械仪器研究所 | Fresh water diatom cultivation method |
| JP2020137514A (en) * | 2019-02-27 | 2020-09-03 | 国立大学法人神戸大学 | Methods for culturing algae, and photobioreactor |
| CN111621422A (en) * | 2019-02-27 | 2020-09-04 | 国立大学法人神户大学 | Method for culturing algae cells and photobioreactor |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201198474Y (en) * | 2008-04-02 | 2009-02-25 | 林健峯 | Apparatus for cultivating oleaginous microalgae |
| CN202022940U (en) * | 2011-03-10 | 2011-11-02 | 金华职业技术学院 | Optical bioreactor for microalgae organism cultivation |
| CN104651234A (en) * | 2015-02-10 | 2015-05-27 | 中国水产科学研究院渔业机械仪器研究所 | Method for culturing high-density fresh water attached diatom |
| CN204477769U (en) * | 2015-04-16 | 2015-07-15 | 杭州富阳光速方舟照明科技有限公司 | With the microalgae culturing device adopting of LED light source |
| CN104789447A (en) * | 2015-04-16 | 2015-07-22 | 杭州富阳光速方舟照明科技有限公司 | Energy-saving microalgae culture device |
-
2016
- 2016-05-19 CN CN201610335590.2A patent/CN105925471A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201198474Y (en) * | 2008-04-02 | 2009-02-25 | 林健峯 | Apparatus for cultivating oleaginous microalgae |
| CN202022940U (en) * | 2011-03-10 | 2011-11-02 | 金华职业技术学院 | Optical bioreactor for microalgae organism cultivation |
| CN104651234A (en) * | 2015-02-10 | 2015-05-27 | 中国水产科学研究院渔业机械仪器研究所 | Method for culturing high-density fresh water attached diatom |
| CN204477769U (en) * | 2015-04-16 | 2015-07-15 | 杭州富阳光速方舟照明科技有限公司 | With the microalgae culturing device adopting of LED light source |
| CN104789447A (en) * | 2015-04-16 | 2015-07-22 | 杭州富阳光速方舟照明科技有限公司 | Energy-saving microalgae culture device |
Non-Patent Citations (2)
| Title |
|---|
| 刘杨平等: "浅谈环境因子对硅藻生长的影响", 《科技信息》 * |
| 庄树宏等: "光强和光质对底栖藻类群落影响Ⅱ .群落和种群的动态和适应模式", 《生态学报》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2020137514A (en) * | 2019-02-27 | 2020-09-03 | 国立大学法人神戸大学 | Methods for culturing algae, and photobioreactor |
| CN111621422A (en) * | 2019-02-27 | 2020-09-04 | 国立大学法人神户大学 | Method for culturing algae cells and photobioreactor |
| US11473051B2 (en) | 2019-02-27 | 2022-10-18 | Nichia Corporation | Method of cultivating algae and photobioreactor |
| JP7534588B2 (en) | 2019-02-27 | 2024-08-15 | 日亜化学工業株式会社 | Method for cultivating algae and photobioreactor |
| CN110184168A (en) * | 2019-05-21 | 2019-08-30 | 荆楚理工学院 | A kind of simple astaxanthin incubator and the method using its culture astaxanthin |
| CN111423985A (en) * | 2020-04-29 | 2020-07-17 | 中国水产科学研究院渔业机械仪器研究所 | Fresh water diatom cultivation method |
| CN111423985B (en) * | 2020-04-29 | 2021-09-24 | 中国水产科学研究院渔业机械仪器研究所 | Fresh water diatom cultivation method |
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Application publication date: 20160907 |