CN110184168A - A kind of simple astaxanthin incubator and the method using its culture astaxanthin - Google Patents
A kind of simple astaxanthin incubator and the method using its culture astaxanthin Download PDFInfo
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- CN110184168A CN110184168A CN201910421709.1A CN201910421709A CN110184168A CN 110184168 A CN110184168 A CN 110184168A CN 201910421709 A CN201910421709 A CN 201910421709A CN 110184168 A CN110184168 A CN 110184168A
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- astaxanthin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
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Abstract
The invention discloses a kind of simple astaxanthin incubators, it includes the cylindric culture vessel of upper end opening, the hollow positioning stem being arranged at the culture vessel center, the pole being arranged in the hollow positioning stem, the bluish violet LED light source lamp being wrapped on the pole and the Stirring device that the locating core column top is arranged in；The flabellum of the positioning stem and Stirring device is transparent material.The invention also discloses the methods for using the simple astaxanthin incubator culture astaxanthin.The invention has the advantages that by the way that positioning stem is arranged in culture vessel, blue-green LED light source lamp is wound on the pole in positioning stem, since positioning stem is transparent configuration, blue-green LED light can be penetrated in the water body that positioning stem is irradiated in culture vessel, promote the culture of astaxanthin.
Description
Technical field
The present invention relates to astaxanthin culture fields, and in particular to a kind of simple astaxanthin incubator and to cultivate shrimp using its green
The method of element.
Background technique
Astaxanthin is a kind of novel biomaterial.Astaxanthin belongs to keto-acid carotenoid, is a kind of terpenes insatiable hunger
And compound, there is relatively strong fat-soluble, not soluble in water, the conjugated double bond chain point formed due to it with hydroxyl and unsaturated ketone group
Minor structure can be supplied to a large amount of unpaired electrons of free radical, to reach the removing of free radical, play significant anti-oxidant function
Effect, so that skin is made to keep uniform color, it is moist, it is full flexible.Natural astaxanthin is the antioxidant of latest generation,
Natural astaxanthin has very high Biomedical function, has the function of resisting age of skin, anti-oxidant, anticancer.By stringent
Animal experiment proves, natural astaxanthin people, aquatic livestock, livestock and poultry etc. are absolutely not poisoned it is without side-effects, so natural astaxanthin is got over
Come more welcome, market prospects are very good, are a kind of well sold and in short supply novel biomaterials.Therefore need to develop culture astaxanthin
Simple and easy method.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of simple astaxanthin incubator and simple astaxanthin culture sides
Method has the characteristics that suitable algae fast high-density growth.
A kind of simple astaxanthin incubator, it includes the cylindric culture vessel of upper end opening, setting in culture appearance
Hollow positioning stem at device center, the pole being arranged in the hollow positioning stem, the royal purple being wrapped on the pole
Color LED light source lamp and the Stirring device that the locating core column top is set；The positioning stem and Stirring device
Flabellum is transparent material.
The positioning stem is an integral molding structure with the culture vessel.
The culture vessel is transparent material.
Locating height is slightly below the height at the top of the culture vessel at the top of the Stirring device.
Culture vessel below the locating core column bottom, which is equipped with, supplies the bluish violet LED light source lamp and Stirring device
The hole that passes through of power supply line.
A method of using simple astaxanthin incubator culture astaxanthin, comprising the following steps:
A, BBM culture solution and algae are placed in the culture vessel of the simple astaxanthin incubator, then in culture vessel
Middle addition tap water simultaneously starts Stirring device and stirs and evenly mixs, and the ratio of tap water and BBM culture solution is 1:1-2；Wherein, described
BBM culture solution using preceding need nitrogen stress handle 8-12 days；
B, the simple astaxanthin incubator is placed in glasshouse, light intensity is controlled by bluish violet LED light source lamp and is maintained
In 6000-8000lux, temperature in glasshouse is maintained 24-26 DEG C, behind algae culture 8-10 days in culture vessel,
Apparent Chinese red to appear can carry out the harvesting of astaxanthin.
The algae is haematococcus pluvialis or green alga.
The invention has the advantages that incubator using the above structure, by the way that positioning stem is arranged in culture vessel, fixed
Blue-green LED light source lamp is wound on pole in the stem of position, since positioning stem is transparent configuration, blue-green LED light can be worn
Positioning stem is irradiated in the water body in culture vessel thoroughly.Light quality is caused by describing light because spectral composition is different
The difference property of illuminating effect.Light quality influences many aspects of haematococcus pluvialis growing, such as photosynthesis, chemical composition, growth
Rate, group, population dynamic and adaptive pattern etc..Different types of light quality can sensitivity to haematococcus pluvialis it is different.?
Under the irradiation of blue green light, content astaxanthin can be best in alga cells accumulation.Green algae group overall growth dynamic is weak
Light-adapted, blue green light responsive type, bluish-green luminous energy efficient activation chlorophyll molecule, thus photosynthetic rate can be accelerated, and lead to algae
The raisings of cell inclusions such as class cell inner chlorophyll and fissional quickening improve the generation astaxanthin of haematococcus pluvialis
Rate.
Detailed description of the invention
Fig. 1 is the structural diagram of the present invention；
Fig. 2 is schematic cross-sectional view of the invention.
Specific embodiment
A kind of simple astaxanthin incubator, it includes the cylindric culture vessel 1 of upper end opening, setting in culture appearance
Hollow positioning stem 2 at 1 center of device, is wrapped on the pole 3 pole 3 being arranged in the hollow positioning stem 2
Bluish violet LED light source lamp 4 and the Stirring device 5 that 2 top of positioning stem is set；The positioning stem 2 and rotation
The flabellum of blender 5 is transparent material.
The positioning stem 2 is an integral molding structure with the culture vessel 1.
The culture vessel 1 is transparent material.
The height at slightly below described 1 top of culture vessel of the locating height in 5 top of Stirring device.
The culture vessel 1 of positioning 2 bottom part down of stem is equipped with to be stirred for the bluish violet LED light source lamp 4 and rotation
Mix the hole that the power supply line of device 5 passes through.
<embodiment 1>
A method of using simple astaxanthin incubator culture astaxanthin, comprising the following steps:
A, BBM culture solution and algae are placed in the culture vessel of the simple astaxanthin incubator, then in culture vessel
Middle addition tap water simultaneously starts Stirring device and stirs and evenly mixs, and the ratio of tap water and BBM culture solution is 1:1；Wherein, described
For BBM culture solution using preceding needing nitrogen stress to handle 8 days, the algae is haematococcus pluvialis or green alga；
B, the simple astaxanthin incubator is placed in glasshouse, light intensity is controlled by bluish violet LED light source lamp and is maintained
In 6000lux, temperature in glasshouse is maintained 24 DEG C, it is to appear apparent behind algae culture 8 days in culture vessel
Chinese red can carry out the harvesting of astaxanthin.
<embodiment 2>
A method of using simple astaxanthin incubator culture astaxanthin, comprising the following steps:
A, BBM culture solution and algae are placed in the culture vessel of the simple astaxanthin incubator, then in culture vessel
Middle addition tap water simultaneously starts Stirring device and stirs and evenly mixs, and the ratio of tap water and BBM culture solution is 1:1.6；Wherein, described
For BBM culture solution using preceding needing nitrogen stress to handle 10 days, the algae is haematococcus pluvialis or green alga；
B, the simple astaxanthin incubator is placed in glasshouse, light intensity is controlled by bluish violet LED light source lamp and is maintained
In 7000lux, temperature in glasshouse is maintained 25 DEG C, it is to appear apparent behind algae culture 9 days in culture vessel
Chinese red can carry out the harvesting of astaxanthin.
<embodiment 3>
A method of using simple astaxanthin incubator culture astaxanthin, comprising the following steps:
A, BBM culture solution and algae are placed in the culture vessel of the simple astaxanthin incubator, then in culture vessel
Middle addition tap water simultaneously starts Stirring device and stirs and evenly mixs, and the ratio of tap water and BBM culture solution is 1:2；Wherein, described
For BBM culture solution using preceding needing nitrogen stress to handle 12 days, the algae is haematococcus pluvialis or green alga；
B, the simple astaxanthin incubator is placed in glasshouse, light intensity is controlled by bluish violet LED light source lamp and is maintained
In 8000lux, temperature in glasshouse is maintained 26 DEG C, it is to appear obvious behind algae culture 10 days in culture vessel
Chinese red can carry out the harvesting of astaxanthin.
The above described is only a preferred embodiment of the present invention, be not intended to limit the present invention in any form, this
The preferred embodiment of invention is not intended to limit the invention, any person skilled in the art, is not departing from skill of the present invention
In the range of art scheme, according to the technical essence of the invention it is to the above embodiments it is any it is simple modification, equivalent variations with
Modification, all of which are still within the scope of the technical scheme of the invention.
Claims (7)
1. a kind of simple astaxanthin incubator, it is characterised in that it include upper end opening cylindric culture vessel (1), setting exist
Pole (3) in hollow positioning stem (2) of hollow positioning stem (2) at culture vessel (1) center, setting,
Rotation of the bluish violet LED light source lamp (4) and setting being wrapped on the pole (3) at the top of positioning stem (2) is stirred
Mix device (5)；The flabellum of positioning stem (2) and Stirring device (5) is transparent material.
2. a kind of simple astaxanthin incubator according to claim 1, it is characterised in that the positioning stem (2) with it is described
Culture vessel (1) is an integral molding structure.
3. a kind of simple astaxanthin incubator according to claim 1, it is characterised in that the culture vessel (1) is transparent
Material.
4. a kind of simple astaxanthin incubator according to claim 1, it is characterised in that at the top of the Stirring device (5)
Locating height is slightly below the height at the top of the culture vessel (1).
5. a kind of simple astaxanthin incubator according to claim 1, it is characterised in that under positioning stem (2) bottom
The culture vessel (1) of side is equipped with the hole passed through for the power supply line of the bluish violet LED light source lamp (4) and Stirring device (5)
Hole.
6. a kind of simple astaxanthin incubator according to claim 1-5, it is characterised in that using described simple
The method of astaxanthin incubator culture astaxanthin, comprising the following steps:
A, BBM culture solution and algae are placed in the culture vessel of the simple astaxanthin incubator, then in culture vessel
Middle addition tap water simultaneously starts Stirring device and stirs and evenly mixs, and the ratio of tap water and BBM culture solution is 1:1-2；Wherein, described
BBM culture solution using preceding need nitrogen stress handle 8-12 days；
B, the simple astaxanthin incubator is placed in glasshouse, light intensity is controlled by bluish violet LED light source lamp and is maintained
In 6000-8000lux, temperature in glasshouse is maintained 24-26 DEG C, behind algae culture 8-10 days in culture vessel,
Apparent Chinese red to appear can carry out the harvesting of astaxanthin.
7. a kind of simple astaxanthin incubator according to claim 6, it is characterised in that the algae is haematococcus pluvialis
Or green alga.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910421709.1A CN110184168A (en) | 2019-05-21 | 2019-05-21 | A kind of simple astaxanthin incubator and the method using its culture astaxanthin |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910421709.1A CN110184168A (en) | 2019-05-21 | 2019-05-21 | A kind of simple astaxanthin incubator and the method using its culture astaxanthin |
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| CN110184168A true CN110184168A (en) | 2019-08-30 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113736665A (en) * | 2021-10-14 | 2021-12-03 | 新疆云藻农业科技发展有限公司 | Haematococcus pluvialis strain breeding method suitable for industrial production and application |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101050419A (en) * | 2007-05-18 | 2007-10-10 | 王逢旦 | Production apparatus for culturing tiny alga and production method |
| JP2008283937A (en) * | 2007-05-21 | 2008-11-27 | Dialight Japan Co Ltd | Photobioreactor |
| CN102337215A (en) * | 2011-10-20 | 2012-02-01 | 烟台华融生物科技有限公司 | Methods for culturing haematococcus pluvialis and producing astaxanthin |
| CN203569085U (en) * | 2013-12-08 | 2014-04-30 | 陕西理工学院 | Oil-producing microalgae culture device |
| CN105925471A (en) * | 2016-05-19 | 2016-09-07 | 海（厦门）生物科技有限公司 | High-density diatom culture dish and diatom culture method |
| CN106754322A (en) * | 2016-12-22 | 2017-05-31 | 山东金晶生物技术有限公司 | A kind of active oxygen bioreactor for microdisk electrode |
| CN207646198U (en) * | 2017-11-10 | 2018-07-24 | 华东理工大学 | A kind of luminous agitating paddle for photosynthesis physiological target |
| CN108753620A (en) * | 2018-05-30 | 2018-11-06 | 昆明理工大学 | A method of improving haematococcus pluvialis biomass and content astaxanthin |
-
2019
- 2019-05-21 CN CN201910421709.1A patent/CN110184168A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101050419A (en) * | 2007-05-18 | 2007-10-10 | 王逢旦 | Production apparatus for culturing tiny alga and production method |
| JP2008283937A (en) * | 2007-05-21 | 2008-11-27 | Dialight Japan Co Ltd | Photobioreactor |
| CN102337215A (en) * | 2011-10-20 | 2012-02-01 | 烟台华融生物科技有限公司 | Methods for culturing haematococcus pluvialis and producing astaxanthin |
| CN203569085U (en) * | 2013-12-08 | 2014-04-30 | 陕西理工学院 | Oil-producing microalgae culture device |
| CN105925471A (en) * | 2016-05-19 | 2016-09-07 | 海（厦门）生物科技有限公司 | High-density diatom culture dish and diatom culture method |
| CN106754322A (en) * | 2016-12-22 | 2017-05-31 | 山东金晶生物技术有限公司 | A kind of active oxygen bioreactor for microdisk electrode |
| CN207646198U (en) * | 2017-11-10 | 2018-07-24 | 华东理工大学 | A kind of luminous agitating paddle for photosynthesis physiological target |
| CN108753620A (en) * | 2018-05-30 | 2018-11-06 | 昆明理工大学 | A method of improving haematococcus pluvialis biomass and content astaxanthin |
Non-Patent Citations (1)
| Title |
|---|
| 张睿钦: ""雨生红球藻细胞转化及虾青素的提取"", 《中国优秀博硕士学位论文全文数据库(硕士) 工程科技Ⅰ辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113736665A (en) * | 2021-10-14 | 2021-12-03 | 新疆云藻农业科技发展有限公司 | Haematococcus pluvialis strain breeding method suitable for industrial production and application |
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