CN106754322A - A kind of active oxygen bioreactor for microdisk electrode - Google Patents
A kind of active oxygen bioreactor for microdisk electrode Download PDFInfo
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- CN106754322A CN106754322A CN201611194836.5A CN201611194836A CN106754322A CN 106754322 A CN106754322 A CN 106754322A CN 201611194836 A CN201611194836 A CN 201611194836A CN 106754322 A CN106754322 A CN 106754322A
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- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
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- C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
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Abstract
The present invention relates to a kind of active oxygen bioreactor for microdisk electrode, belong to technical field of microalga biology.Reactor disclosed by the invention contains the inner barrel device of active oxygen generation, and its inner cylinder disposed inboard has light source, and the photocatalyst coating of transparent hydrophobic is smeared in outside.Photocatalyst can control the water in algae solution and oxygen catalytic generation active oxygen, the generation for realizing active oxygen by Illumination adjusting, so as to promote the synthesis of frustule target metabolic product, and suppress the growth of other invasive species.And photocatalyst will not be consumed as catalyst in incubation, its hydrophobic property is avoided that the adherent growth of algae, reactor, culture medium can also be carried out disinfection treatment by active oxygen before and after culture, with the obvious advantage compared to traditional bioreactor.
Description
Technical field
The present invention relates to technical field of microalga biology, a kind of active oxygen photo-biological for microdisk electrode is specifically provided anti-
Answer device.
Background technology
Haematococcus pluvialis are a kind of fresh water monoplast green algas, are subordinate to Chlorophyta, Chlorophyceae, volvocales, haematococcus section, red ball
Trentepohlia.Haematococcus pluvialis make frond that red, therefore named haematococcus or haematococcus pluvialis are presented because largely can accumulate astaxanthin.Its quilt
Be considered in nature to produce the optimal biological platform of astaxanthin, using the natural astaxanthin good quality of Haematococcus pluvialis production,
Antioxidation activity is strong.The existing forms of algae are green nourishing to haematococcus pluvialis generally in the environment that illumination is weaker, nitrogen phosphorus is abundant
Cell, quick fission, intracellular content astaxanthin is relatively low；Under stress conditions such as light irradiation high, reactive oxygen species
Balance between synthesis and detoxication is broken, and the active oxygen quenching system in haematococcus pluvialis cell starts, and its Prawn is blue or green
Active oxygen can be consumed in procatarxis building-up process and largely synthesize in the cell and accumulate.Therefore, active oxygen is in frustule
Accumulation is the key factor for promoting chemical activators.
In the production of haematococcus pluvialis large-scale cultivation, the induction of astaxanthin is generally carried out using sunshine in outdoor.Though
The sunshine of right high intensity can generate active oxygen with inducing cell, promote the synthesis of astaxanthin, but be subject to day in process of production
According to factors such as, weather, seasons, the generation of active oxygen is unstable, causes astaxanthin yield unstable.And manually add active oxygen and lure
Method action time of raw agent is short, strong toxicity, high cost, it is difficult to realize scale application.Article《Active oxygen is to haematococcus pluvialis
Growth and the influence of content astaxanthin》（Ecological science 2006,25（3）, 213-215）In inquired into and induced by adding active oxygen
Agent methylene blue can significantly improve content astaxanthin.However, methylene blue has toxicity, environment, animal, human body can all be made
Into harm, it is difficult to apply in large-scale production.
Accordingly, it would be desirable to develop a kind of dress that active oxygen can be continuously generated in Astaxanthin In Haematococcus Pluvialis synthesis phase
Put, to improve the yield of astaxanthin in large-scale cultivation.
The content of the invention
It is an object of the invention to provide a kind of active oxygen bioreactor for microdisk electrode, by application photocatalyst
Material photocatalytically persistently produces the characteristic of active oxygen, so as to promote the accumulation of Astaxanthin In Haematococcus Pluvialis.Use
Technical scheme is as follows：
A kind of active oxygen bioreactor for microdisk electrode, including bioreactor main body, air inlet, gas outlet,
Inlet, liquid outlet, gas distributor and at least one photocatalyst inner barrel device；Gas distributor is placed in bioreactor master
The bottom of body, and be connected with the air inlet, stirring is circulated to algae solution by blasting the air containing carbon dioxide；Feed liquor
Mouth is located at the side top of the bioreactor main body, for being inoculated with the injection with culture medium；After culture terminates, algae solution is led to
Cross the liquid outlet discharge of bioreactor bottom part body；Exhaust outlet is located on the bioreactor main body top cover, is used for
Discharge waste gas；Photocatalyst inner barrel device is fixed on bioreactor main body top cover lower section by glass cement adhesion or flange.
Preferably, bioreactor main body is column bioreactor, flat plate photobioreactor, duct type
One kind in bioreactor.
Preferably, bioreactor main body, its material be glass, lucite, stainless steel, sealed polyethylene plastic,
One kind in polyvinyl chloride plastic film, polyacrylic film.
Preferably, photocatalyst inner barrel device is made up of bottom lock, open-topped transparent pipe and built-in light source.
Preferably, light source is fixed on transparent tube side wall by glass cement, and by the externally connected light intensity of power line
On controller, transparent pipe outer wall has the photocatalyst coating of transparent hydrophobic.
Preferably, the photocatalyst coating of transparent hydrophobic contain but be not limited to nano titanium oxide, it is nano silicon, dilute
Release agent, dispersant, organic siliconresin.
Preferably, transparent pipe material is in glass, lucite, the plastic sheeting of tape frame structure, quartz glass
Kind.
Preferably, light source is one or more in LED, fluorescent lamp, uviol lamp, incandescent lamp, Halogen lamp LED, sodium vapor lamp.
Preferably, light source is the LED containing ultraviolet chip.
, before microalgae is cultivated, first be added to culture medium in bioreactor by the reactor assembly that the present invention is provided,
The intensity of illumination in photocatalyst inner barrel device is opened to maximum by controller, excites photocatalyst to produce active oxygen, to culture medium
Carried out disinfection sterilization with reactor.Light source is closed in sterilization after terminating, and stands a period of time after after active oxygen decomposition failure, by mesh
Mark algae kind be seeded to reactor, in incubation, according to microalgae in different growth phases for active oxygen tolerance degree not
Together, the intensity and irradiation time of light source, the speed of control active oxygen generation, it is to avoid excessive at the induction initial stage are adjusted by controller
Active oxygen to cause frustule to be bleached dead.
The invention discloses a kind of active oxygen bioreactor for microdisk electrode, the reactor contains active oxygen life
Into inner barrel device, its inner cylinder disposed inboard has a light source, and the photocatalyst coating of transparent hydrophobic is smeared in outside.Shone in inner cylinder inside light
Under the conditions of, the photocatalyst on the outside of inner cylinder by the water in algae solution and oxygen catalytic generation active oxygen, and can be discharged into algae solution.It is logical
Generating rate, concentration, the control of duration that the intensity of illumination, wave band, frequency can be realized to active oxygen are overregulated, so that
Promote the synthesis of frustule astaxanthin, while other invasive species such as bacterium, chytrid, miscellaneous algae, protozoic life can also be suppressed
It is long.In addition, photocatalyst coating is used as catalyst, will not be consumed in incubation, also terminate rear frond and light in the absence of culture
The problem that catalyst is separate, while its hydrophobic property can be effectively prevented from the adherent growth of algae, can also be by work before and after culture
Property oxygen carries out disinfection treatment to reactor, culture medium.
Brief description of the drawings
Below in conjunction with the accompanying drawings and implementation method the present invention is further detailed explanation：
Fig. 1 is the active oxygen column bioreactor structural representation for microdisk electrode of the invention；
Fig. 2 is the active oxygen flat plate photobioreactor structural representation for microdisk electrode of the invention；
Fig. 3 is the active oxygen duct type bioreactor structural representation for microdisk electrode of the invention.
Wherein：1st, bioreactor main body, 2, photocatalyst inner barrel device, 3, air inlet, 4, gas outlet, 5, inlet,
6th, liquid outlet, 7, gas distributor, 8, light source, 9, photocatalyst coating, 10, bioreactor main body top cover, 11, power line,
12nd, light intensity control.
Specific embodiment
Further detailed description is done to the present invention with reference to embodiment, but is merely to illustrate the present invention rather than limit
The scope of the present invention processed.Other are any without departing from the change made under Spirit Essence of the invention and principle, modification, replacement, knot
Close, simplify, be equivalents, equally fall within the application appended claims limited range.
Embodiment 1
As shown in Figure 1, a kind of active oxygen column bioreactor for microdisk electrode that the present invention is provided, including it is vertical
Pillar bioreactor 1, photocatalyst inner barrel device 2, air inlet 3, gas outlet 4, inlet 5, liquid outlet 6, gas distributor
7th, light source 8, photocatalyst coating 9, reactor head 10, power line 11, light intensity control 12.
Column bioreactor 1 is cylindrical structural, is made up of lucite, 150 cm of height, the cm of internal diameter 30,
Gas distributor 7 is installed in bottom, and gas distributor 7 is connected with air inlet 3, and liquid outlet 6 is located at reactor lowermost end.In photocatalyst
Wound packages is put 2 and is made up of bottom lock, open-topped transparent quartz glass tube, the cm of length 100, the cm of internal diameter 10, containing ultraviolet
The LED light source 8 of chip is fixed on quartz glass tube inner side by glass cement, and by power line 11 and the phase of light intensity control 12
Even, uniform application contains nano titanium oxide, nano silicon, diluent, dispersant, organosilicon tree on the outside of quartz glass tube
The transparent photo-catalytic coating 9 of fat.Quartz ampoule is adhered to the lower section of bioreactor glass roof 10 by glass cement.
BG-11 nitrogen stresies culture medium is added to bioreactor 1 by inlet 5, by light intensity control 12 by light
Opened to maximum according to intensity, excite photocatalyst coating 9 to produce active oxygen, sterilizing is carried out to culture medium and reactor 1.Sterilization 3 ~
6 h, can thoroughly kill bacterium, miscellaneous algae, protozoan in reactor 1 and culture medium etc..Sterilization closes LED light source 5 after terminating,
And 1 ~ 2 h is stood, and active oxygen decomposition failure is treated, haematococcus pluvialis are seeded in reactor 1, inoculum density is 0.31 g/L,
25 ~ 30 DEG C of cultivation temperature, built-in light source 5 control intensity of illumination in 100 ~ 300 μm of ol photon m by light intensity control 12-2∙
s-1, blasted by gas distributor 7 by air inlet 3 and contain 2%（v/v）The air of carbon dioxide is circulated stirring to algae solution.
After indoor culture 10 days, frustule content astaxanthin（w/w）3.63% is reached, cellular biomass reaches 1.26 g/L, compared to nothing
The control group bioreactor of photocatalyst inner barrel device design, astaxanthin yield improves 42.3%.
Embodiment 2
As shown in Figure 2, a kind of active oxygen flat plate photobioreactor for microdisk electrode that the present invention is provided, overall knot
Structure design such as embodiment 1, difference is that reactor 1 is made up of clear glass, and length is respectively 150 cm, 20 cm, 120
Cm, 3 groups long 70 cm, the photocatalyst inner barrel devices 2 of the cm of internal diameter 10 are adhered to bioreactor glass roof 10 with glass cement
Lower section.
Haematococcus pluvialis inoculation operation is 0.22 g/L with embodiment 1, inoculum density, and built-in light source 5 is by light intensity control
12 control intensities of illumination are in 50 ~ 100 μm of ol photon m-2 s-1.After reactor 1 is placed in into outside scenery 10 days, frustule
Content astaxanthin（w/w）3.02.% is reached, cellular biomass reaches 0.86 g/L, compared to what is designed without photocatalyst inner barrel device
Control group bioreactor, astaxanthin yield improves 22.7%.
Embodiment 3
As shown in Figure 3, a kind of active oxygen duct type bioreactor for microdisk electrode that the present invention is provided, overall knot
Such as embodiment 1, difference is that reactor 1 is made of stainless steel for structure design, 100 cm of height, internal diameter 40 cm, 2 groups long 50 cm,
The photocatalyst inner barrel device 2 of the cm of internal diameter 10 flange is fixed on the lower section of bioreactor stainless steel top cover 10.Reactor 1 connects
The glass pipe that internal diameter is 5 cm is connect, algae solution Cyclic culture is carried out using membrane pump.
Haematococcus pluvialis inoculation operation is 0.56 g/L with embodiment 1, inoculum density, and built-in light source 5 is by light intensity control
12 control intensities of illumination are in 80 ~ 200 μm of ol photon m-2∙s-1.After reactor 1 is placed in into outside scenery 10 days, frustule
Content astaxanthin（w/w）4.68.% is reached, cellular biomass reaches 2.35 g/L, compared to what is designed without photocatalyst inner barrel device
Control group bioreactor, astaxanthin yield improves 30.6%.
In above-described embodiment incubation, bacterium, fungi, fast-growing are not found by micro- sem observation algae solution upgrowth situation
The miscellaneous algae of property and protozoic pollution, there is not the phenomenon of adherent growth in inner cylinder hyaline-quartz pipe outer wall, and control group is without hydrophobic
Adherent growth is serious on the outside of the inner cylinder quartz ampoule of photocatalyst coating, have impact on light transmittance.The active oxygen photo-biological that the present invention is provided
Reactor is substantially better than traditional bioreactor.
Furthermore, it is necessary to illustrate, the specific embodiment described in this specification, its each several part title etc. can not
Together, the equivalent or simple change that all constructions according to described in inventional idea of the present invention, feature and principle are done, is included in the present invention
In the protection domain of patent.Those skilled in the art described specific embodiment can be made an amendment or
Supplement is substituted using similar mode, without departing from structure of the invention or surmounts model defined in the claims
Enclose, protection scope of the present invention all should be belonged to.
Claims (9)
1. a kind of active oxygen bioreactor for microdisk electrode, including bioreactor main body, air inlet, outlet
Mouth, inlet, liquid outlet, gas distributor and at least one photocatalyst inner barrel device, it is characterised in that：The gas distributor
The bottom of bioreactor main body is placed in, and is connected with the air inlet, by blasting the air containing carbon dioxide to algae
Liquid is circulated stirring；The inlet is located at the side top of the bioreactor main body, for inoculation and culture medium
Injection；After culture terminates, algae solution is discharged by the liquid outlet of bioreactor bottom part body；The exhaust outlet is located at photoproduction
On thing reactor body top cover, for discharging waste gas；The photocatalyst inner barrel device is fixed by glass cement adhesion or flange
In bioreactor main body top cover lower section.
2. according to a kind of active oxygen bioreactor for microdisk electrode described in claim 1, it is characterised in that：It is described
Bioreactor main body be column bioreactor, flat plate photobioreactor, duct type bioreactor
In one kind.
3. according to a kind of active oxygen bioreactor for microdisk electrode described in claim 1, it is characterised in that：It is described
Bioreactor main body, its material is thin glass, lucite, stainless steel, sealed polyethylene plastic, igelite
One kind in film, polyacrylic film.
4. according to a kind of active oxygen bioreactor for microdisk electrode described in claim 1, it is characterised in that：It is described
Photocatalyst inner barrel device be made up of bottom lock, open-topped transparent pipe and built-in light source.
5. according to a kind of active oxygen bioreactor for microdisk electrode described in claim 4, it is characterised in that：It is described
Light source be fixed on transparent tube side wall by glass cement, it is transparent and by the externally connected light intensity control of power line
Pipe outer wall has the photocatalyst coating of transparent hydrophobic.
6. according to a kind of active oxygen bioreactor for microdisk electrode described in claim 5, it is characterised in that：It is described
Transparent hydrophobic photocatalyst coating contain but be not limited to nano titanium oxide, nano silicon, diluent, dispersant,
Organic siliconresin.
7. according to a kind of active oxygen bioreactor for microdisk electrode described in claim 5, it is characterised in that：It is described
Transparent pipe material be the one kind in glass, lucite, the plastic sheeting of tape frame structure, quartz glass.
8. according to a kind of active oxygen bioreactor for microdisk electrode described in claim 5, it is characterised in that：It is described
Light source be one or more in LED, fluorescent lamp, uviol lamp, incandescent lamp, Halogen lamp LED, sodium vapor lamp.
9. according to a kind of active oxygen bioreactor for microdisk electrode described in claim 8, it is characterised in that：It is described
Light source be the LED containing ultraviolet chip.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2019076004A (en) * | 2017-10-20 | 2019-05-23 | 清水建設株式会社 | Algae culture method and algae culture plant |
| CN110184168A (en) * | 2019-05-21 | 2019-08-30 | 荆楚理工学院 | A kind of simple astaxanthin incubator and the method using its culture astaxanthin |
| CN113862119A (en) * | 2021-11-12 | 2021-12-31 | 西安建筑科技大学 | Microalgae photobioreactor |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010004868A (en) * | 2008-05-30 | 2010-01-14 | Ccs Inc | Culturing system for aquatic photosynthesizing organism |
| CN203128547U (en) * | 2013-03-29 | 2013-08-14 | 上海海洋大学 | Simple device for continuously and efficiently culturing microalgae |
| CN106148470A (en) * | 2016-09-28 | 2016-11-23 | 山东金晶生物技术有限公司 | A kind of method utilizing photocatalyst to promote Haematocoocus Pluvialls accumulation astaxanthin |
-
2016
- 2016-12-22 CN CN201611194836.5A patent/CN106754322A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010004868A (en) * | 2008-05-30 | 2010-01-14 | Ccs Inc | Culturing system for aquatic photosynthesizing organism |
| CN203128547U (en) * | 2013-03-29 | 2013-08-14 | 上海海洋大学 | Simple device for continuously and efficiently culturing microalgae |
| CN106148470A (en) * | 2016-09-28 | 2016-11-23 | 山东金晶生物技术有限公司 | A kind of method utilizing photocatalyst to promote Haematocoocus Pluvialls accumulation astaxanthin |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2019076004A (en) * | 2017-10-20 | 2019-05-23 | 清水建設株式会社 | Algae culture method and algae culture plant |
| CN110184168A (en) * | 2019-05-21 | 2019-08-30 | 荆楚理工学院 | A kind of simple astaxanthin incubator and the method using its culture astaxanthin |
| CN113862119A (en) * | 2021-11-12 | 2021-12-31 | 西安建筑科技大学 | Microalgae photobioreactor |
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Inventor after: Dai Yunfa Inventor after: Li Qian Inventor after: Zheng Yurui Inventor after: Wang Hua Inventor after: Zheng Yubin Inventor after: Chen Shulin Inventor before: Dai Yunfa Inventor before: Li Qian Inventor before: Zheng Yurui Inventor before: Wang Hua Inventor before: Zheng Yubin |
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Application publication date: 20170531 |