CN109133354B - Algae-based ecological bacteriostasis method for wastewater treatment - Google Patents
Algae-based ecological bacteriostasis method for wastewater treatment Download PDFInfo
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- CN109133354B CN109133354B CN201810662314.6A CN201810662314A CN109133354B CN 109133354 B CN109133354 B CN 109133354B CN 201810662314 A CN201810662314 A CN 201810662314A CN 109133354 B CN109133354 B CN 109133354B
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- 241000195493 Cryptophyta Species 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000004065 wastewater treatment Methods 0.000 title claims abstract description 16
- 239000002351 wastewater Substances 0.000 claims abstract description 23
- 241000195628 Chlorophyta Species 0.000 claims abstract description 15
- 238000005286 illumination Methods 0.000 claims abstract description 5
- 238000012258 culturing Methods 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims description 17
- 239000011550 stock solution Substances 0.000 claims description 2
- 239000012224 working solution Substances 0.000 claims description 2
- 229940079593 drug Drugs 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 239000002994 raw material Substances 0.000 abstract 1
- 101150100654 pacC gene Proteins 0.000 description 11
- 239000000126 substance Substances 0.000 description 11
- 235000015097 nutrients Nutrition 0.000 description 9
- 241000282898 Sus scrofa Species 0.000 description 7
- 244000284012 Vetiveria zizanioides Species 0.000 description 7
- 235000007769 Vetiveria zizanioides Nutrition 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 244000052616 bacterial pathogen Species 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 229910052698 phosphorus Inorganic materials 0.000 description 4
- 230000029553 photosynthesis Effects 0.000 description 4
- 238000010672 photosynthesis Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 241001673669 Porcine circovirus 2 Species 0.000 description 2
- 241000607142 Salmonella Species 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 239000003899 bactericide agent Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000029058 respiratory gaseous exchange Effects 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 206010005003 Bladder cancer Diseases 0.000 description 1
- 241000790338 Dictyococcus Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000566145 Otus Species 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 208000007097 Urinary Bladder Neoplasms Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000003501 co-culture Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000003673 groundwater Substances 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 201000005112 urinary bladder cancer Diseases 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Botany (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Environmental & Geological Engineering (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Water Supply & Treatment (AREA)
- Biomedical Technology (AREA)
- Environmental Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention provides an algae-based ecological bacteriostasis method for wastewater treatment, which comprises the following steps: (1) preparing a culture solution: taking waste water as a culture solution; (2) subpackaging the culture solution: subpackaging the culture solution into each cultivation container; (3) planting crops: planting a crop in a cultivation container; (4) inoculating green algae: inoculating the green algae into the culture solution at the concentration of 0.125 g/L; (5) in the illumination of 180 mu mol m‑2s‑1Culturing is carried out under the conditions that the average temperature is 28.3 ℃ and the relative humidity is 36.7-85.2%. The wastewater treated by the method completely reaches the discharge standard, the produced crops have high yield and excellent quality, and microalgae with considerable yield can be additionally obtained, and the microalgae can be used as important raw materials of biological energy sources and biological medicines and have important social and economic values.
Description
Technical Field
The invention belongs to the field of wastewater treatment, and particularly relates to an algae-based ecological bacteriostasis method for wastewater treatment.
Background
The wastewater from pig farm contains high concentration of nutrients (such as NH)4-N, P). The current wastewater treatment technologies mainly comprise: physical/chemical treatment processes (high cost); microbial treatment processes (which require large energy input and are not suitable for treating wastewater containing high concentrations of nutrients); these techniques produce large amounts of sediment (e.g., sludge) causing secondary pollution that is difficult to dispose of. Related studies have shown that some plants and microalgae can effectively remove N, P from wastewater without producing precipitates, but because of their slow growth, they do not meet the need for efficient and rapid wastewater treatment.
Another challenge faced in swine wastewater treatment is the removal of pathogenic bacteria. Research has shown that even after treatment (e.g., anaerobic nitrification), swine wastewater contains Salmonella (Salmonella), Escherichia coli (E.coli), and Porcine circovirus type 2 (Porcine circovirus-2) and many other microorganisms, which pollute groundwater, soil, food and pose a threat to humans and animals. The methods for removing pathogens from water bodies mainly comprise the following steps: (1) chemical disinfection method: however, the bacteria can generate drug resistance to different bactericides, and the drinking water can be chlorinated due to the large-scale use of the bactericides, so that the probability of the human suffering from bladder cancer is increased; (2) ultraviolet (UV) sterilization: the high-concentration organic substances and a large amount of suspended matters in the pig raising wastewater can weaken the penetrating power of UV in water, so that the sterilization effect is not obvious; (3) an ozone method: the cost is high.
Disclosure of Invention
The invention aims to provide a method for simultaneously removing high-concentration nutrient substances in pig wastewater and inactivating pathogenic bacteria aiming at the defects of the prior art.
The purpose of the invention is realized by the following technical scheme: an algae-based ecological bacteriostasis method for wastewater treatment comprises the following steps:
(1) preparing a culture solution: taking waste water as a culture solution;
(2) subpackaging the culture solution: subpackaging the culture solution into each cultivation container;
(3) planting crops: planting a crop in a cultivation container;
(4) inoculating green algae: inoculating the green algae into the culture solution at the concentration of 0.125 g/L;
(5) in the illumination of 180 mu mol m-2s-1Culturing is carried out under the conditions that the average temperature is 28.3 ℃ and the relative humidity is 36.7-85.2%.
Further, the culture solution is a wastewater stock solution or is diluted according to a set proportion and then is used as a working solution.
Furthermore, the crops are fixed at the bottle openings of the cultivation containers through sponge strips.
Further, the volume of the cultivation container is 23L.
Further, the cultivation container is a light-permeable container.
Further, the inoculated green algae is a green algae-Dictyosphaerium sp obtained from the waste water.
According to the invention, based on the mutual action of species of algae and crops, the algae and the crops are cultured in the same system, the growth and photosynthesis of the algae are promoted by nutrient substances in the system and carbon dioxide released by the respiration of crop root systems, and the development of the crop root systems and the absorption of nutrient substances and water in the system are promoted by oxygen released by the photosynthesis of the algae, so that the removal efficiency of the nutrient substances in the system is greatly improved; and the active oxygen (ROS) released by the microalgae in the growth cycle can effectively kill pathogens in the system. The invention has the following beneficial effects: (1) the yield of crops and algae is improved, and the wastewater treatment time is shortened; (2) when crops and green algae are harvested, high-concentration nutrient substances and pathogens in the wastewater are effectively removed, the yield is high, and the economic benefit is high; (3) only illumination and organic matters are needed, the use of chemical substances and extra energy and the generation of side effects are avoided, and the pollution to the environment is reduced.
Detailed Description
The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Modifications or substitutions of the methods, steps or conditions of the present invention may be made without departing from the spirit and substance of the invention, and the technical means used in the examples are conventional means known to those skilled in the art unless otherwise specified.
Example (b):
this example uses a green algae-vetiver co-culture system to treat swine wastewater.
(1) Culture solutions were prepared in three groups of culture vessels (3 per group, 23L culture vessel capacity): diluting the wastewater according to a ratio of 1:3 by taking water as a solute; the three groups are respectively an AC group (algae + culture solution), a PC group (crops + culture solution) and a PACC group (crops + algae + culture solution).
(2) Planting crops: fixing vetiver grass at mouths of 6 cultivation containers of a PC and PACC group by using sponge strips, and planting one strain in each container;
(3) inoculating green algae, screening green algae from wastewater, inoculating the green algae into 6 cultivation containers of AC and PACC groups at concentration of 0.125g/L, and adding no green algae in PC group;
(4) placing the three groups of cultivation containers in 180 mu mol m of light-2s-1Culturing at 28.3 deg.C and 36.7-85.2% relative humidity, and measuring green algae biomass, vetiver growth and NH in culture solution at intervals4N, P concentration and microbial species. NH in initial wastewater4A concentration of-N of 102.2mg/L, by 15 days of culture, detected NH in PC group4Concentration of-N87.5 mg/L, NH in group AC4Concentration of-N27.1 mg/L, NH in PACC group4The concentration of-N was 3.5 mg/L. To NH4The culture is finished when the content of-N, P is respectively reduced to 5mg/L and 2mg/L, and the vetiver grass of the PACC group grows better than that of the PC group. Through detection, in the culture period, the average growth rate of 3 vetiver grass strains in the PACC group is 12.8 +/-1.2 g FW/d, and the average production rate of the PC group is 1.5 +/-1.0 g FW/d; after the culture is finished, the average dry weight of the roots of the vetiver grass in the PACC group is 57.4 +/-14.5 g/plant, while the average dry weight of the roots of the vetiver grass in the PC group is 33.2 +/-7.6 g/plant; the green algae biomass in the PACC group was higher than that in the AC group, and the average relative growth rates of the algae were 0.168. + -. 0.009 and 0.151. + -. 0.007, respectively.
When NH is present in the culture broth4The average time taken by the PACC/AC/PC groups at N concentrations of less than 5mg/L was 13.3/34.2/65.5d, respectively; when the concentration of P is less than 2mg/L, the average time of PACC/AC/PC groups is 11.6/25.5/82.7 d. Detection of microorganisms in PACC group culture broth showed that when the culture time was 15 days, all pathogenic bacteria were substantially removed and the relative abundance of other microorganisms than a few bacteria was also significantly reduced (see Table 1).
Table 1 relative abundance of bacteria (OTUs) over time during swine wastewater treatment with vetiver and dictyococcus. Data are mean ± SD (n ═ 3).
As can be seen from the examples, the invention is based on the mutual action of algae and crop species, the algae and crop are cultured in the same system, the two promote each other, the carbon dioxide released by the respiration of the root system of the crop promotes the growth and photosynthesis of the algae, and the oxygen released by the photosynthesis of the algae promotes the development of the root system of the crop, the absorption of nutrients and water and the growth of the crop, therefore, the crop and the algae can be fast and quickly cultured in the waste water culture solutionThe growth is fast, and the crops and algae absorb a large amount of nutrient elements in the growth process, thereby accelerating NH in the wastewater4-N, P removal; in addition, free Radicals (ROS) released by the microalgae in the growth cycle can effectively inactivate pathogenic bacteria in the wastewater, thereby reducing the cost of wastewater treatment and improving the treatment efficiency. The wastewater can be effectively treated only by providing illumination and organic matters without adding other chemical substances and additional energy input, and the crop and microalgae can be cultured simultaneously, so that the method has the advantages of simple system composition, simple and convenient operation and management and extremely high output.
Claims (4)
1. An algae-based ecological bacteriostasis method for wastewater treatment is characterized by comprising the following steps:
(1) preparing a culture solution: taking pig raising wastewater as a culture solution;
(2) subpackaging the culture solution: subpackaging the culture solution into each light-permeable cultivation container;
(3) planting crops: planting a crop in a cultivation container;
(4) inoculating green algae: inoculating the green algae into the culture solution at a concentration of 0.125g/L, wherein the inoculated green algae is Chlorella-Glomulina taken from the wastewaterDictyosphaerium sp；
(5) In the illumination of 180 mu mol m-2 s-1Culturing is carried out under the conditions that the average temperature is 28.3 ℃ and the relative humidity is 36.7-85.2%.
2. The method for ecological bacteriostasis of algae-based for wastewater treatment as claimed in claim 1, wherein the culture solution is a wastewater stock solution or is diluted according to a set proportion and then used as a working solution.
3. The algal-based ecological bacteriostasis method for wastewater treatment as claimed in claim 1, wherein the crop is fixed at the mouth of the cultivation container through a sponge strip.
4. The algal-based ecological bacteriostasis method for wastewater treatment according to claim 1, wherein the volume of the cultivation container is 23L.
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| CN109133354B true CN109133354B (en) | 2020-11-20 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1834035A (en) * | 2006-04-04 | 2006-09-20 | 中山大学 | Method of treating organic tin compound in sewage by utilizing solidified algae pellets |
| CN104719110A (en) * | 2015-02-15 | 2015-06-24 | 浙江大学 | Ecological water planting method based on crop-algae mutual growing function |
| CN108192889A (en) * | 2018-01-11 | 2018-06-22 | 中南民族大学 | A kind of method of bacteria cellulose immobilized microalgae processing waste water |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2997959B1 (en) * | 2012-11-09 | 2016-04-01 | Invivo Nsa | USE OF SUGAR IN A MICROALGAE CULTURE TO DECREASE THEIR SELF-FLOCCULATION |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1834035A (en) * | 2006-04-04 | 2006-09-20 | 中山大学 | Method of treating organic tin compound in sewage by utilizing solidified algae pellets |
| CN104719110A (en) * | 2015-02-15 | 2015-06-24 | 浙江大学 | Ecological water planting method based on crop-algae mutual growing function |
| CN108192889A (en) * | 2018-01-11 | 2018-06-22 | 中南民族大学 | A kind of method of bacteria cellulose immobilized microalgae processing waste water |
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