CN106145382A - A kind of preparation method of the microbial water-purifying agent for fishery cultivating water body - Google Patents
A kind of preparation method of the microbial water-purifying agent for fishery cultivating water body Download PDFInfo
- Publication number
- CN106145382A CN106145382A CN201610552448.3A CN201610552448A CN106145382A CN 106145382 A CN106145382 A CN 106145382A CN 201610552448 A CN201610552448 A CN 201610552448A CN 106145382 A CN106145382 A CN 106145382A
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- China
- Prior art keywords
- water
- purifying agent
- preparation
- microbial
- bacillus cereus
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Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/38—Organic compounds containing nitrogen
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/14—NH3-N
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/15—N03-N
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/26—H2S
Abstract
The invention discloses a kind of microbial water-purifying agent for fishery cultivating water body, described microorganism water treatment agent in culturing water is the expanded soil particle of activation being fixed with and condensing dead leaf bacillus cereus.Present invention also offers the preparation method of above-mentioned microorganism water treatment agent in culturing water.Microorganism water treatment agent in culturing water of the present invention uses expanded soil as fixation support, and to condense dead leaf bacillus cereus for immobilization object, by with cultivating increment, immobilized bacterium amount is big, and bioaccumulation efficiency is high；Material used is the most nontoxic, non-secondary pollution, and with low cost, it is simple to transports and stores, can realize large-scale production.
Description
Technical field
The invention belongs to the production technology of water purification agent, be specifically related to a kind of microbial water-purifying agent for fishery cultivating water body
Preparation method.
Background technology
The deterioration of breeding environment has become as the key factor of restriction China culture fishery healthy and sustainable development.Therefore,
Urgent need to seek a kind of effective, method repairing pond culture environment of strong operability and technology.Traditional physics or
Chemical method mainly uses absorption, precipitates, filters and the form such as drug disinfection, utilizes physics or chemical method not only to also exist
Purify the shortcomings such as thorough, recycling rate variance, input cost are big, there is also antibiotic etc residual and aquatic animal body
The generation of interior Resistant strain, final initiation aquatic products edible safety sex chromosome mosaicism.In recent years, microorganism is utilized to pollute to pond of degrading
Thing, carries out the method for cultivating pool water remediation and causes and pay close attention to widely, and achieve significant achievement.
Condense the bacillus cereus that dead leaf bacillus cereus is a kind of homofermentative lactic, Gram-positive, lactic acid producing, peroxidating
Hydrogen enzyme is positive, phenylalanine deaminase negative, can form spore.Bacillus coagulans can starch-splitting, sucrose, Ah
Drawing uncle's sugar, xylose and the various saccharides such as lactose, maltose, decomposition glucose produces acid not aerogenesis.Owing to having lactic acid bacteria simultaneously
With the characteristic of bacillus cereus, acid-resistant and anti-high-temperature, there is abundant exoenzyme class, and thalline itself is nontoxic, therefore at Aquatic product
Aquaculture pond water quality has obtained certain application on purifying, and can significantly reduce ammonia nitrogen in pond waters, nitrite nitrogen, sulfuration
Hydrogen and the content of solubility total phosphorus.But current research concentrates on free Bacillus coagulans, owing to it is at discharge condition
Easily washed away by current down, easily ingested by other biological under hydrostatic condition, and free Bacillus coagulans cannot natural subsidence
To the water-deposit interface of cultivating pool water body, limit its ability repairing bottom material of pond for breeding.Actually this has become as shadow
Ring a key factor of Bacillus coagulans application effect.
Owing to bottom material of pond for breeding serves not only as cultivation water holding pond, the storage vault of various chemical substance, or plant, animal and
The habitat of microorganism and nutrient recirculation center, so the interaction of bottom material of pond for breeding and water can affect water consumingly
Matter, therefore wants to support good water, is necessary for setting about from bottom material of pond for breeding improvement, it is necessary to fully pay attention to the management of bottom material of pond for breeding.
Summary of the invention
In order to make up the deficiencies in the prior art, the invention provides a kind of microbial water-purifying agent for fishery cultivating water body
Preparation method, the microbial water-purifying agent of the present invention with low cost, easy and simple to handle and can realize large-scale production breeding water body use
Microbial water-purifying agent, it is intended to after uniformly putting into fishery cultivating pond, can fall to bottom material of pond for breeding, purify repair cultivating pool water-
Deposit interface pollutant.
The technical solution adopted in the present invention is as follows:
A kind of microbial water-purifying agent for fishery cultivating water body, described water purification agent is the work being fixed with dead leaf bacillus cereus
The bentonite clay particle changed, its preparation method comprises the following steps:
(1) bentonite is stirred in distilled water standing, steep 20～30h, then sieve；
(2) stand after sieving, settle 20～30h, extract the suspension more than 12cm degree of depth under liquid level；
(3) suspension got in step (2) is centrifuged, is dried, sieve and obtain the bentonite clay particle of activation；
(4) bentonite clay particle that the condensation dead leaf bacillus cereus and step (3) that are in exponential phase prepare is blended in
Together, cultivate under aerobic conditions；
(5) at 20～35 DEG C, cultivate 20～40h, after air-drying, obtain the microbial water-purifying agent for fishery cultivating water body.
Further, the sieve that step (1) is crossed is 150～200 mesh:
Further, the dead leaf bacillus cereus that condenses in step (4) is the volume ratio according to 1:1～4 with bentonite clay particle
Mix.
The microbial water-purifying agent of the present invention is mainly being sprinkling upon the water of cultivating pool water body-deposit interface, according to difference
The cultivation phase, usage amount is 60～120g/m2。
The invention has the beneficial effects as follows:
1. the microbial water-purifying agent of fishery cultivating water body uses activation Pengization native as fixation support, to condense dead leaf bud
Spore bacillus is immobilization object, and by with cultivating increment, immobilized bacterium amount is big, and bioaccumulation efficiency is high；
2. condense the activity that dead leaf bacillus cereus still can keep higher after immobilization；
3. the expanded soil after activation has bigger absorption property, can more efficient absorption water-deposit interface
Pollutant, are beneficial to the condensation dead leaf bacillus cereus degraded to pollutant after fixing, improve the effect of absorption degradation pollutant
Efficiency；
4. the material used by is the most nontoxic, non-secondary pollution, and with low cost, it is simple to transports and stores, and can realize
Large-scale production.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described further:
Embodiment 1
The preparation method of a kind of microbial water-purifying agent for fishery cultivating water body, comprises the following steps:
(1) bentonite is stirred in distilled water standing, steep 24h, then sieve；
(2) stand after sieving, settle 25h, extract the suspension more than 12cm degree of depth under liquid level；
(3) suspension got in step (2) is centrifuged, is dried, sieve and obtain the bentonite clay particle of activation；
(4) bentonite clay particle that the condensation dead leaf bacillus cereus and step (3) that are in exponential phase prepare is blended in
Together, cultivate under aerobic conditions；
(5) at 26 DEG C, cultivate 30h, after air-drying, obtain the microbial water-purifying agent for fishery cultivating water body.
At the crucian cultivation initial stage according to 100g/m2Usage amount, above-mentioned water purification agent is sprinkling upon crucian cultivation pond equably,
Water purification agent can sink to the water at the bottom of pond-deposit interface, reaches the purpose in purifying aquaculture pond.
Experiment shows, compares with the condensation dead leaf bacillus cereus adding free state, adds the fixing condensation of activated expanded soil
Water at the bottom of pond, dead leaf bacillus cereus experimental group pond-deposit interface ammonia-nitrogen content have dropped 30.2%, and nitrite nitrogen contains
Amount have dropped 33.5%, and hydrogen sulfide content have dropped 18.6%, and total phosphorus content have dropped 44.0%, containing of organic carbon and organic nitrogen
Amount have dropped 20.6% and 20.3% respectively.
Embodiment 2
The preparation method of a kind of microbial water-purifying agent for fishery cultivating water body, comprises the following steps:
(1) bentonite is stirred in distilled water standing, steep 30h, then sieve；
(2) stand after sieving, settle 30h, extract the suspension more than 12cm degree of depth under liquid level；
(3) suspension got in step (2) is centrifuged, is dried, sieve and obtain the bentonite clay particle of activation；
(4) bentonite clay particle that the condensation dead leaf bacillus cereus and step (3) that are in exponential phase prepare is blended in
Together, cultivate under aerobic conditions；
(5) at 35 DEG C, cultivate 40h, after air-drying, obtain the microbial water-purifying agent for fishery cultivating water body.
At the crucian cultivation initial stage according to 100g/m2Usage amount, above-mentioned water purification agent is sprinkling upon crucian cultivation pond equably,
Water purification agent can sink to the water at the bottom of pond-deposit interface, reaches the purpose in purifying aquaculture pond.
Experiment shows, compares with the condensation dead leaf bacillus cereus adding free state, adds the fixing condensation of activated expanded soil
Water at the bottom of pond, dead leaf bacillus cereus experimental group pond-deposit interface ammonia-nitrogen content have dropped 25.2%, and nitrite nitrogen contains
Amount have dropped 30.5%, and hydrogen sulfide content have dropped 22.3%, and total phosphorus content have dropped 39.5%, containing of organic carbon and organic nitrogen
Amount have dropped 25.6% and 26.3% respectively.
Claims (3)
1. the microbial water-purifying agent for fishery cultivating water body, it is characterised in that described water purification agent is for being fixed with dead leaf bud
The bentonite clay particle of the activation of spore bacillus, its preparation method comprises the following steps:
(1) bentonite is stirred in distilled water standing, steep 20～30h, then sieve；
(2) stand after sieving, settle 20～30h, extract the suspension more than 12cm degree of depth under liquid level；
(3) suspension got in step (2) is centrifuged, is dried, sieve and obtain the bentonite clay particle of activation；
(4) bentonite clay particle that the condensation dead leaf bacillus cereus and step (3) that are in exponential phase prepare is mixed,
Cultivate under aerobic conditions；
(5) at 20～35 DEG C, cultivate 20～40h, after air-drying, obtain the microbial water-purifying agent for fishery cultivating water body.
The preparation method of microbial water-purifying agent the most according to claim 1, it is characterised in that the sieve that step (1) is crossed is
150～200 mesh.
The preparation method of microbial water-purifying agent the most according to claim 1, it is characterised in that the condensation in step (4) is withered
Leaf bacillus cereus and bentonite clay particle are to mix according to the volume ratio of 1:1～4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610552448.3A CN106145382A (en) | 2016-07-12 | 2016-07-12 | A kind of preparation method of the microbial water-purifying agent for fishery cultivating water body |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610552448.3A CN106145382A (en) | 2016-07-12 | 2016-07-12 | A kind of preparation method of the microbial water-purifying agent for fishery cultivating water body |
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| CN106145382A true CN106145382A (en) | 2016-11-23 |
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|---|---|---|---|
| CN201610552448.3A Pending CN106145382A (en) | 2016-07-12 | 2016-07-12 | A kind of preparation method of the microbial water-purifying agent for fishery cultivating water body |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106542651A (en) * | 2016-12-20 | 2017-03-29 | 大连赛姆生物工程技术有限公司 | A kind of microorganism water treatment agent in culturing water and preparation method thereof |
| CN106542652A (en) * | 2016-12-20 | 2017-03-29 | 大连赛姆生物工程技术有限公司 | A kind of carrier immobilized microbial water-purifying agent and preparation method thereof |
| CN108033574A (en) * | 2017-12-27 | 2018-05-15 | 广州市水电建设工程有限公司 | A kind of Ecological water purification agent for city river purification and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002325569A (en) * | 2001-02-21 | 2002-11-12 | Hitachi Plant Eng & Constr Co Ltd | Immobilized support, heated support and method for producing the same and method for cleaning environment by using the immobilized support and the heated support |
| CN101935105A (en) * | 2010-07-22 | 2011-01-05 | 浙江工商大学 | Microbial purifying agent for aquaculture water and preparation method thereof |
-
2016
- 2016-07-12 CN CN201610552448.3A patent/CN106145382A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002325569A (en) * | 2001-02-21 | 2002-11-12 | Hitachi Plant Eng & Constr Co Ltd | Immobilized support, heated support and method for producing the same and method for cleaning environment by using the immobilized support and the heated support |
| CN101935105A (en) * | 2010-07-22 | 2011-01-05 | 浙江工商大学 | Microbial purifying agent for aquaculture water and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106542651A (en) * | 2016-12-20 | 2017-03-29 | 大连赛姆生物工程技术有限公司 | A kind of microorganism water treatment agent in culturing water and preparation method thereof |
| CN106542652A (en) * | 2016-12-20 | 2017-03-29 | 大连赛姆生物工程技术有限公司 | A kind of carrier immobilized microbial water-purifying agent and preparation method thereof |
| CN108033574A (en) * | 2017-12-27 | 2018-05-15 | 广州市水电建设工程有限公司 | A kind of Ecological water purification agent for city river purification and preparation method thereof |
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Application publication date: 20161123 |