CN110002611A - A kind of breeding water body regulator and preparation method thereof - Google Patents
A kind of breeding water body regulator and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of breeding water body regulator, which includes the component of following mass fraction: 10-15 parts of photosynthetic bacteria, 40-50 parts of lactic acid bacteria, 10-20 parts of saccharomycete, 10-15 parts of actinomyces, 10-20 parts of der Pilz, 5-10 parts of glucose, 1-5 parts of microelement, 1-5 parts of vitamin.The nutrition regulating agent is made by series of steps such as solid medium culture, fluid nutrient medium culture, ferment tank, filtering and concentrating, allotments.The present invention provides a kind of excellent breeding water body regulator, the easy to operate, good purification using regulator improvement breeding water body.
Description
Technical field
The present invention relates to microorganism water purification processing technology field, more particularly to a kind of breeding water body regulator and
Preparation method.
Background technique
In recent years, with the development of the aquaculture models such as China's culture fishery intensive culture, half intensive culture, intensive degree is increasingly mentioned
It is high.But China's aquaculture production is substantially or hydrostatic, not blowdown, the aquaculture model based on bait throwing in, residual bait, excrement,
The eutrophy factor such as aquatic animals and plants corpse coexists in a water body, in addition the pollution at water source and pond self-cleaning and regulating power
It reduces, severely exceeds the indexs such as nitrate nitrogen in breeding water body, chemical oxygen consumption (COC), ammonia nitrogen, cultured water, reactive phosphate, disease
Pathogenic microorganism type and quantity rise, and breeding environment deterioration causes fish and shrimp diseases to occur again and again, bring greatly to breeding production
Harm.
Currently, the method for improving breeding water body mainly has cultivation filter-feeder fish shellfish, artificial decision algae sociales, plantation water
Raw higher plant and placement bio-carrier.But the disadvantages of there are cumbersome, effect is unsatisfactory, investment is big.And it is sharp
Carrying out bioelectric detecting to cultivation water with microorganism is to improve breeding ecological environment, reduce disease generation and keep aquaculture strong
Health, the important means of stable development.
Therefore it provides the microbial bacteria of a kind of easy to operate, good purification, the small quick purifying aquatic water of investment
The problem of agent is those skilled in the art's urgent need to resolve.
Summary of the invention
In view of this, the present invention provides a kind of easy to operate, good purifications, the small quick purifying aquaculture water of investment
The breeding water body regulator of body.
To achieve the goals above, the present invention adopts the following technical scheme:
A kind of breeding water body regulator, including microbial bacterial agent and nutritional agents；
The microbial bacterial agent includes the component of following mass fraction: 10-15 parts of photosynthetic bacteria, 40-50 parts of lactic acid bacteria, ferment
Female bacterium 10-20 parts, 10-15 parts of actinomyces and 10-20 parts of der Pilz；
The nutritional agents includes the component of following mass fraction: 5-10 parts of glucose, 1-5 parts of microelement and vitamin 1-
5 parts.
Microorganism by the effects of oxidation, reduction, photosynthetic, assimilation, alienation residual bait, excrement, aquatic animals and plants corpse point
Mineralising is solved, is converted into simple inorganic matter, while environment purification, guarantee water body normal function, maintenance aquatic products from complicated organic matter
The ecological balance of breeding environment.In this complicated substance cyclic process, beneficial microbe is followed by influence carbon cycle, nitrogen
Ring, phosphorus circulation, sulfur cycle etc. and have an effect.During carbon cycle, beneficial microbe decomposing organic matter discharges CO2, through floating
The complicated organic matter for swimming the photosynthesis synthesis building autologous tissue of plant, provides for zooplankter, zoobenthos, fish and shrimp
O2And polysaccharose substance.In nitrogen cycle, microorganism passes through ammoniation, nitrification, denitrification, nitrogen fixation,
Under the conditions of dissolved oxygen is abundant, by water body ammoniacal nitrogen and the toxic mineralized nitrogen such as nitrite nitrogen be nitrate nitrogen, by algae benefit
With to play the role of purifying water.In phosphorus circulation, microorganism makes the phosphorus of solubilised state in water body be suspended granular absorption shape
At particle cluster flow, switch to precipitate through flocculation reaction, the content of available phosphorus in water body is reduced, to control the eutrophication and indigo plant of water
The flourish of the toxic algaes such as algae.In addition, bioavailable content of organics is lower or scarce in water body during water remediation
When weary nitrogen and phosphorus element, repairing effect is poor, add certain nutriment can reinforce it is biological prosthetic.
Preferably, the microbial bacterial agent includes the component of following mass fraction: 12 parts of photosynthetic bacteria, 30 parts of lactic acid bacteria,
15 parts of saccharomycete, 12 parts of actinomyces and 15 parts of der Pilz；The nutritional agents includes the component of following mass fraction: 7 parts of glucose,
3 parts and 3 parts of vitamin of microelement.
Preferably, the photosynthetic bacteria is one of Rhodopseudomonas palustris, Rhodospirillum rubrum, Yunnan Rhodococcus sp or more
Kind.
Photosynthetic bacteria can be cooked carbon source, hydrogen donor using small organic molecule during self-reproduction, molten using water environment
Solution nitrogen (such as ammonium, nitrate, nitrite) does nitrogen source synthesis organic nitrogen compound, therefore the small molecule that can be consumed in water is organic
Object, ammonium, nitrate, nitrite play a part of to purify water.
Preferably, the lactic acid bacteria is one of Lactobacillus rhamnosus, lactobacillus paracasei, lactobacillus acidophilus or more
Kind.
Lactic acid bacteria can decompose using the nitrogenous organic macromolecule material in water, thus the product of ammonia nitrogen is controlled from source
It is tired, and the small molecule nitrogenous compound in water body more effectively can be directly absorbed and utilized in photosynthetic bacteria, to effectively remove ammonia
Nitrogen.Also, lactic acid bacteria is improved Vfa Concentration by biological antagonist and generates organic acid, to reduce pH value, is hindered
Only and inhibit pathogenic bacteria intrusion and field planting, maintain the normal ecological balance.
Preferably, the saccharomycete is one of saccharomyces cerevisiae, ocean rhodotorula, brewer's yeast or a variety of.
Saccharomycete is that organic macromolecule material is first hydrolyzed into small point by the hydrolase on surface using the mechanism of action of organic matter
Sub- organic matter, then pyruvic acid is translated by glycolytic pathway, and production capacity supplies saccharomycete.Fermented type yeast will pass through wine
Essence fermentation converts pyruvic acid into ethyl alcohol, and oxidized form saccharomycete in mitochondria by conversion of pyruvate at acetylcoenzyme, then lead to
Triphosphoric acid conversion and cycle is crossed into small-molecule substances such as carbon dioxide and water.
Preferably, the actinomyces are one of streptomyces microflavus, bacillus subtilis or a variety of.
Preferably, the der Pilz is Sphaerotilus natans.
Preferably, the microelement is one of potassium, calcium, sodium, magnesium, aluminium, zinc, copper, manganese, sulphur, iron or a variety of.
Preferably, the vitamin is one of vitamin A, vitamin B, vitamin C, vitamin E or a variety of.
Lack the necessary nutrient of microorganism by addition polluted water body: microelement, vitamin can promote
The growth and breeding of bacterium, enhancement microbiological degradation capability improve water body purification effect.
A kind of breeding water body regulator, preparation method include the following steps:
(1) photosynthetic bacteria, lactic acid bacteria, saccharomycete, actinomyces and der Pilz are inoculated in respectively on solid medium and are carried out
Activation；
(2) it is inoculated in corresponding fluid nutrient medium and is cultivated respectively with the oese single colonie on plate of making even, obtain liquid
Microbial inoculum seed liquor；
(3) seed liquor is respectively connected to ferment in fermentor, bacterium solution is collected in concentration, mixed according to required ratio
It closes, nutritional agents is added, obtains breeding water body regulator.
It can be seen via above technical scheme that compared with prior art, the beneficial effects of the present invention are: being capable of fast decoupled
Residual bait, pesticide residue, larger molecular organics matter in breeding water body etc., degradation of ammonia nitrogen, reactive phosphate, cultured water, nitric acid
The substances such as nitrogen increase the dissolved oxygen amount of water body, create the good environment of an activation for aquatic animal.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
Microorganism raw material sources in embodiment:
Rhodopseudomonas palustris: Rhodopseudomonas palustris, CGMCC 1.2180 is purchased from Chinese common micro-
Biological inoculum preservation administrative center；
Rhodospirillum rubrum: Rhodospirillum rubrum, CGMCC 1.3369 is purchased from China General Microbiological strain
Preservation administrative center；
Yunnan Rhodococcus sp: Rhodococcus yunnanensis, CGMCC 4.3558 is purchased from China General Microbiological bacterium
Kind preservation administrative center；
Lactobacillus rhamnosus: Lactobacillus Rhamnosus, CGMCC 1.484 is purchased from China General Microbiological bacterium
Kind preservation administrative center；
Lactobacillus paracasei: Lactobacillus paracasei, CGMCC 1.9089 is purchased from China General Microbiological
Culture presevation administrative center；
Lactobacillus acidophilus: Lactobacillus acidophilus, CGMCC 1.1878 is purchased from China General Microbiological
Culture presevation administrative center；
Saccharomyces cerevisiae: Saccharomyces cerevisiae Hansen, CAS number: 68876-77-7 is purchased from Zhengzhou
Kang Yuan chemical products Co., Ltd；
Ocean rhodotorula: Rhodotorual glutinis, CGMCC 2.703 is purchased from China General Microbiological strain guarantor
Hide administrative center；
Brewer's yeast: Saccharomyces cerevisiae, CGMCC 3217 is purchased from China General Microbiological strain guarantor
Hide administrative center；
Streptomyces microflavus: Streptomyces Microflavus, CGMCC 4.0891 is purchased from China General Microbiological bacterium
Kind preservation administrative center；
Bacillus subtilis: Bacillus subtilis, CGMCC 1.7417 is purchased from China General Microbiological strain guarantor
Hide administrative center；
Sphaerotilus natans: Sphaerotilusnatans, article No.: it is limited to be purchased from the towering biotechnology in Shanghai Shanghai by GIM1.350
Company.
Embodiment 1
(1) nutritional agents for weighing following mass fraction is spare: glucose 5g, KCl 2g, CaCl22.7g、NaCl 2.5g、
Vitamin A 1g, vitamin B 1g；
(2) Rhodopseudomonas palustris, Lactobacillus rhamnosus, saccharomyces cerevisiae, streptomyces microflavus and football shirt bacterium are inoculated with respectively
In being activated on solid medium；
Photosynthetic bacteria solid medium are as follows: sodium acetate 1.145g, peptone 0.055g, sodium bicarbonate 0.6g, thiosulfuric acid
Sodium 0.4g, sodium chloride 0.3g, magnesium sulfate 0.1g, potassium dihydrogen phosphate 0.05g, distilled water 1000ml；
Lactic acid bacteria solid medium are as follows: beef extract 3g, peptone 3g, yeast extract 3g, lactose 20g, agar 15g, distilled water
1000ml；
Saccharomycete solid medium are as follows: yeast extract 10g, peptone 20g, glucose 20g, agar powder 20g, distilled water
1000ml；
Actinomyces solid medium are as follows: starch 5g, yeast extract 2g, sucrose 10g, NaCl 2g, peptone 2g, K2HPO4
0.5g、MgSO4·7H2O 10g, glucose 0.5g, CaCO31g, soybean powder soak juice 1000ml；
Der Pilz solid medium are as follows: soybean powder 35g, starch 45g, glucose 1g, corn flour 10g, peptone 4g, KNO3
0.2g、(NH4)28O4 1g、CaCl2 0.01g、CaCO34g, distilled water 1000ml.
(3) it is inoculated in corresponding fluid nutrient medium and is cultivated respectively with the oese single colonie on plate of making even, obtain liquid
Microbial inoculum seed liquor；
Photosynthetic bacteria liquid culture medium are as follows: sodium acetate 2.0g, ammonium chloride 1.0g, sodium bicarbonate 0.5g, sodium chloride 1.0g, phosphorus
Acid dihydride potassium 0.2g, magnesium chloride hexahydrate 0.2g, T.M liquid storage 1ml, yeast extract 0.8g, distilled water 1000ml；
Lactic acid bacteria fluid nutrient medium are as follows: peptone 10g, beef extract 10g, yeast extract 5g, glucose 20g, sodium acetate 5g, lemon
Lemon acid diammonium hydrogen 2g, Tween 80 1.0ml, dipotassium hydrogen phosphate 2g, epsom salt 0.2g, seven water manganese sulfate 0.05g, distilled water
1000ml；
Saccharomycete fluid nutrient medium are as follows: DEXTROSE ANHYDROUS 9g, molasses 45g, wheat bran 36g, corn distiller's dried grain and its soluble matter
3g, Cottonseed Meal 2.75g, yeast extract 0.75g, (NH3)2SO40.8g、KH2PO43g、MgSO40.3g、CaCl20.3g, distilled water
1000ml；
Actinomyces fluid nutrient medium are as follows: peptone 5g, sodium chloride 5g, glucose 10g, soluble starch 10g, distilled water
1000ml；
Der Pilz fluid nutrient medium are as follows: potato 200g is manually cut into the block of 2cm × 2cm, boiling water boiling 30min, gauze mistake
Filter, leaves and takes filtered juice, and glucose 20g is added in filtered juice, is settled to 1000ml.
(4) seed liquor is respectively connected to ferment in fermentor, bacterium solution is collected in concentration；
Fermentation by Photosynthetic Bacteria culture medium: yeast extract 3g, peptone 3g, MgSO4·7H2O 0.5g、CaCl2·2H2O
0.3g, distilled water 1000ml；
Lactobacillus-fermented culture medium: glucose 10g, yeast extract 5g, peptone 5g, diammonium hydrogen citrate 2g, tween
801ml, sodium acetate 5g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.58g, manganese sulfate 0.25g, distilled water 1000ml；
Saccharomycetes to make fermentation culture medium: corn flour 15g, wheat bran 4g, KH2PO40.6g, urea 0.6g, distilled water 1000ml；
Actinomycete fermentation culture medium: yeast extract 10g, beef extract 10g, glucose 10g, glycerol 15g, MgSO4·
7H2O 0.5g、FeSO4·7H2O 0.01g, distilled water 1000ml；
Filamentous bacteria fermentation culture medium: starch 30g, glucose 20g, soybean cake powder 20g, peptone 5g, fish meal 5g, (NH)28O4 1.5g、MgSO4 1g、KH2PO40.4g、CaCO30.4g, soya-bean oil 10g, distilled water 1000ml.
(5) according to Rhodopseudomonas palustris bacterium solution 10g, Lactobacillus rhamnosus bacterium solution 40g, saccharomyces cerevisiae bacterium solution 10g, thin Huang
The mixing of the ratio of streptomycete bacterium solution 10g and football shirt bacterium bacterium solution 10g is added nutritional agents and mixes, obtains breeding water body regulator.
Embodiment 2
(1) nutritional agents for weighing following mass fraction is spare: glucose 10g, MgSO45g、Al2O32g、ZnSO42.5g、
CuSO42.5g, vitamin C 2g, vitamin E 2g；
(2) Rhodospirillum rubrum, lactobacillus paracasei, ocean rhodotorula, bacillus subtilis and football shirt bacterium are inoculated with respectively
In being activated on solid medium；
(3) it is inoculated in corresponding fluid nutrient medium and is cultivated respectively with the oese single colonie on plate of making even, obtain liquid
Microbial inoculum seed liquor；
(4) seed liquor is respectively connected to ferment in fermentor, filtering and concentrating collects thallus；
(5) according to Rhodospirillum rubrum bacterium solution 15g, lactobacillus paracasei bacterium solution 50g, ocean rhodotorula bacterium solution 20g, withered grass bud
The mixing of the ratio of spore bacillus bacterium solution 15g and football shirt bacterium bacterium solution 20g is added nutritional agents and mixes, obtains breeding water body regulator.
Wherein each medium component is the same as embodiment 1.
Embodiment 3
(1) nutritional agents for weighing following mass fraction is spare: glucose 7g, MnO 2.5g, FeO1.3g, vitamin A 2g,
Vitamin C 1g, vitamin E 2g；
(2) Yunnan Rhodococcus sp, lactobacillus acidophilus, brewer's yeast, bacillus subtilis and football shirt bacterium are inoculated in respectively solid
It is activated on body culture medium；
(3) it is inoculated in corresponding fluid nutrient medium and is cultivated respectively with the oese single colonie on plate of making even, obtain liquid
Microbial inoculum seed liquor；
(4) seed liquor is respectively connected to ferment in fermentor, filtering and concentrating collects thallus；
(5) according to Yunnan Rhodococcus sp bacterium solution 12g, lactobacillus acidophilus bacterium solution 30g, brewer's yeast bacterium solution 15g, bacillus subtilis
The mixing of the ratio of bacterium bacterium solution 12g and football shirt bacterium bacterium solution 15g is added nutritional agents and mixes, obtains breeding water body regulator.
Wherein each medium component is the same as embodiment 1.
Experiment 1
Experimental system:
The seawater dark place 72h static reserve after the filter of second level sand is tested, experiment container is Glass fibre reinforced plastic tub, there is independent plumbing
System and perfect air supply system, Indoor Natural illumination, whole process inflation.Glass fibre reinforced plastic tub, cultivation are sterilized with potassium permanganate before experiment
It is sterilized with water through disinfection agent of chlorine dioxide, using aeration, mode removes the chlorine residue in water body for 24 hours thereafter.Prawn opens after temporarily supporting 7 days
Begin to test, every group is put in a suitable place to breed 300 tail of prawn at random, and daily bait throwing in 4 times, feeding volume is ingested according to prawn depending on situation, experimentation
In add fresh water supplement evaporated water in right amount, remaining management measure each group is consistent.
Experimental method: 21 days experimental periods, application situation are as follows:
Experimental group 1: according to 2g/m3Apply 1 sample of embodiment；
Experimental group 2: according to 2g/m3Apply 2 sample of embodiment；
Experimental group 3: according to 2g/m3Apply 3 sample of embodiment；
Control group: it does not apply.
The method of sampling: sampling is primary before not being administered, and application same day sampling is primary, and sampling in 7 days is primary after application, 14 after application
Its sampling is primary, and sampling in 20 days is primary after application.
Measure content: nitrate nitrogen, ammonia nitrogen, cultured water, chemical oxygen consumption (COC), the variation feelings of reactive phosphate in monitoring water body
Condition.
Measuring method:
The measurement of nitrate nitrogen:
(1) drafting of standard curve: draw potassium nitrate standard solution (10 μ g/ml) 0,0.1,0.2,0.4,0.6,0.8,
1.0ml adds pure water to 4.0ml, each pipe enriching hydrochloric acid 0.2ml, phenol solution 0.1ml, mixes, sulphur is added in 25ml colorimetric cylinder
Acid solution 6.0ml, shakes up, and places 10min, and using 0 pipe as reference, absorbance is measured at 390hm wavelength, draws standard curve.
(2) sample measures: draw in water sample 2ml and 25ml colorimetric cylinder (can dilute processing) if necessary, add pure water extremely
4.0ml, the drafting of the same below standard curve.
(3) calculate: nitrate nitrogen (mg/l)=A/V, A are nitrate nitrogen standard content, and V is water sampling volume.
The measurement of ammonia nitrogen:
(1) drafting of standard curve: 0,0.5,1.0,3.0,5.0,7.0 and 10.0ml ammonium standard solution is drawn in 50ml
Colorimetric cylinder adds water to graticule, adds 1.0ml potassium sodium tartrate solution, mixes.Add 1.5ml nessler reagent, mixes.Place 10min
Afterwards, at wavelength 420nm, absorbance is measured.
(2) measurement of water sample: divide and take the water sample after flocculation precipitation pretreatment (processing can be diluted if necessary) in right amount, add
Enter in 50ml colorimetric cylinder, be diluted to graticule, adds 1.0ml potassium sodium tartrate solution, the drafting of the same below standard curve.According to standard
Curve calculates ammonia-nitrogen content.
The measurement of cultured water:
(1) 2ml aluminum hydroxide suspension can be added if any color and suspended matter in water sample in every 100ml water sample, stir,
It stands, filtering.
(2) take 50ml colorimetric cylinder be separately added into 0.5 μ g/ml cultured water standard solution 0,0.5,1.0,2.0,3.0,
4.0,5.0,6.0,8.0,10.0ml are diluted with water to 50.0ml, and 1.0ml p-aminobenzene sulfonic acid solution is added, and mix, after 5min
Respectively plus 1ml sodium acetate solution and 1ml hydrochloric acid naphthalidine solution, the measurement absorbance value in wavelength 520nm at is mixed after 30min, is drawn
Standard curve processed.
(3) it takes and is adjusted in neutral water sample 50ml (processing can be diluted if necessary) addition cuvette, the same below standard curve
Drafting.The content of cultured water in water sample is calculated according to standard curve.
The measurement of chemical oxygen consumption (COC):
(1) water sample for taking 100ml to mix well is in 250ml conical flask；
(2) 5ml sulfuric acid solution is added to shake up, 10ml0.01mol/l KMnO is accurately added with buret4Standard solution, and
3-4 beades；
(3) in heating the timing since emitting first air pocket on electric furnace, 10min is accurately boiled；
(4) conical flask is removed, 2min is placed, drops to 76 DEG C or so to temperature, 0.01mol/l is accurately added with buret
Na2C2O4Standard solution 15ml uses 0.01mol/l KMnO immediately4Standard solution is titrated to lightpink 30s and does not move back as terminal, note
Record consumed KMnO4The volume V of solution1(ml)；
(5)KMnO4The measurement of standard solution correction coefficient (K): in the solution titrated above, 0.01mol/ is added
lNa2C2O4Standard solution 15ml uses 0.01mol/l KMnO immediately4Standard solution is titrated to lightpink 30s and does not move back as terminal, note
Record consumed KMnO4The volume V of solution2(ml)。
K=15.0/V2
(6) chemical oxygen consumption (COC) is calculated as follows:
The measurement of reactive phosphate:
(1) foundation of standard curve: draw 0 respectively, 0.5,1.0,1.5,2.0,2.5,3.0,4.0ml phosphate standard makes
With liquid in 50ml color-comparison tube, it is diluted to 50ml with deionized water, it is molten that 1ml ascorbic acid is added into each colorimetric cylinder respectively
Liquid mixes, and after 30s plus 2ml molybdate solution mixes well, and after being placed at room temperature for 15min, measures absorbance value at 700 nm, draws
Standard curve processed.
(2) sample measures: testing by pretreated sample by Specification Curve of Increasing step, phase is found on standard curve
The phosphorus content answered simultaneously calculates concentration.
Experimental result:
Experimental result is as shown in table 1- table 5.
1 nitric acid nitrogen content of table
As seen from the results in Table 1, before application with the application same day, nitric acid nitrogen content experimental group and control group difference is not in water body
Obviously.Application starts after 7 days, and nitric acid nitrogen content control group is apparently higher than experimental group in water body.
2 ammonia-nitrogen content of table
As seen from the results in Table 2, with the application same day before application, ammonia-nitrogen content experimental group and control group difference and unknown in water body
It is aobvious.After application 7 days, ammonia-nitrogen content control group is apparently higher than experimental group in water body.
3 cultured water content of table
As seen from the results in Table 3, before application with the application same day, cultured water content experimental group and control group difference is simultaneously in water body
It is unobvious.After application 7 days, cultured water content control group is apparently higher than experimental group in water body.
4 chemical oxygen consumption (COC) of table
As seen from the results in Table 4, chemical oxygen consumption (COC) experimental group and control group difference is not before application and the application same day, in water body
Obviously.After application 7 days, chemical oxygen consumption (COC) control group is apparently higher than experimental group in water body.
5 reactive phosphate content of table
As seen from the results in Table 5, before application and on the day of being administered, reactive phosphate content experimental group and control group difference in water body
It is not obvious.After application 7 days, reactive phosphate content control group is apparently higher than experimental group in water body.
Each embodiment in this specification is described in a progressive manner, the highlights of each of the examples are with other
The difference of embodiment, the same or similar parts in each embodiment may refer to each other.For device disclosed in embodiment
For, since it is corresponded to the methods disclosed in the examples, so description is relatively simple, related place is said referring to method part
It is bright.
The general principles defined herein can without departing from the spirit or scope of the present invention, in other realities
It applies in example and realizes.Therefore, the present invention is not intended to be limited to embodiment illustrated herein, and be to fit to it is disclosed herein
Principle and the consistent widest scope of features of novelty.
Claims (10)
1. a kind of breeding water body regulator, which is characterized in that including microbial bacterial agent and nutritional agents；
The microbial bacterial agent includes the component of following mass fraction: 10-15 parts of photosynthetic bacteria, 40-50 parts of lactic acid bacteria, saccharomycete
10-20 parts, 10-15 parts of actinomyces and 10-20 parts of der Pilz；
The nutritional agents includes the component of following mass fraction: 5-10 parts of glucose, 1-5 parts of microelement and 1-5 parts of vitamin.
2. a kind of breeding water body regulator according to claim 1, which is characterized in that the microbial bacterial agent includes as follows
The component of mass fraction: 12 parts of photosynthetic bacteria, 30 parts of lactic acid bacteria, 15 parts of saccharomycete, 12 parts of actinomyces and 15 parts of der Pilz；It is described
Nutritional agents includes the component of following mass fraction: 7 parts of glucose, 3 parts of microelement and 3 parts of vitamin.
3. a kind of breeding water body regulator according to claim 1, which is characterized in that the photosynthetic bacteria is the red vacation in marsh
One of monad, Rhodospirillum rubrum, Yunnan Rhodococcus sp are a variety of.
4. a kind of breeding water body regulator according to claim 1, which is characterized in that the lactic acid bacteria is rhamnose cream bar
One of bacterium, lactobacillus paracasei, lactobacillus acidophilus are a variety of.
5. a kind of breeding water body regulator according to claim 1, which is characterized in that the saccharomycete be saccharomyces cerevisiae,
One of ocean rhodotorula, brewer's yeast are a variety of.
6. a kind of breeding water body regulator according to claim 1, which is characterized in that the actinomyces are thin yellow strepto-
One of bacterium, bacillus subtilis are a variety of.
7. a kind of breeding water body regulator according to claim 1, which is characterized in that the der Pilz is sphaerotilus natans
Bacterium.
8. a kind of breeding water body regulator according to claim 1, which is characterized in that the microelement be potassium, calcium,
One of sodium, magnesium, aluminium, zinc, copper, manganese, sulphur, iron are a variety of.
9. a kind of breeding water body regulator according to claim 1, which is characterized in that the vitamin is vitamin A, dimension
One of raw element B, vitamin C, vitamin E are a variety of.
10. a kind of breeding water body regulator as described in claim 1-9 is any, preparation method include the following steps:
(1) photosynthetic bacteria, lactic acid bacteria, saccharomycete, actinomyces and der Pilz are inoculated in respectively on solid medium and are activated；
(2) it is inoculated in corresponding fluid nutrient medium and is cultivated respectively with the oese single colonie on plate of making even, obtain liquid bacterial agent
Seed liquor；
(3) seed liquor being respectively connected to ferment in fermentor, bacterium solution is collected in concentration, it is mixed according to required ratio,
Nutritional agents is added, obtains breeding water body regulator.
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Application publication date: 20190712 |