CN106542651A - A kind of microorganism water treatment agent in culturing water and preparation method thereof - Google Patents
A kind of microorganism water treatment agent in culturing water and preparation method thereof Download PDFInfo
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- CN106542651A CN106542651A CN201611184476.0A CN201611184476A CN106542651A CN 106542651 A CN106542651 A CN 106542651A CN 201611184476 A CN201611184476 A CN 201611184476A CN 106542651 A CN106542651 A CN 106542651A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/327—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae characterised by animals and plants
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/101—Sulfur compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/14—NH3-N
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/18—PO4-P
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/20—Total organic carbon [TOC]
Abstract
The invention discloses a kind of microorganism water treatment agent in culturing water and preparation method thereof, the microorganism water treatment agent in culturing water is the activated diatomaceous earth granule for being fixed with Bacillus licheniformis.Present invention also offers the preparation method of above-mentioned microorganism water treatment agent in culturing water., using activated diatomaceous earth as fixation support, with Bacillus licheniformis as immobilization object, by rising in value with culture, immobilization bacterium amount is big, and bioaccumulation efficiency is high for microorganism water treatment agent in culturing water of the present invention；Bacillus licheniformis not only can still keep higher activity Jing after immobilization, can be also protected from other biological by activated diatomaceous earth and ingest, and can sink to rapidly pond water bottom water deposit interface and play a role and purify water；Kieselguhr after activation has bigger absorption property, can be with the pollutant of more efficient absorption water sediment interface；Material used is all nontoxic, non-secondary pollution, and with low cost, is readily transported and stores, and is capable of achieving large-scale production.
Description
Technical field
The invention belongs to water body purification field, is related to a kind of microorganism water treatment agent in culturing water and preparation method thereof.
Background technology
The deterioration of breeding environment has become the key factor of restriction China culture fishery healthy and sustainable development.Therefore,
Urgent need will seek a kind of methods and techniques of the reparation pond culture environment of effective, strong operability.Traditional physics or
Chemical method mainly using forms such as absorption, precipitation, filtration and drug disinfections, is not only existed using physics or chemical method
The shortcomings of purification is not thoroughly, recycling rate of waterused is poor, input cost is big, also there is antibiotic etc residual and aquatic animal body
The generation of interior Resistant strain, it is final to cause aquatic products edible safety sex chromosome mosaicism.In recent years, using microorganism come pond pollution of degrading
Thing, the method for carrying out cultivating pool water remediation cause extensive concern, and achieve significant achievement.
Microorganism has important effect and status in the biological group of nature, and it is both that Producer can utilize luminous energy
Or simple inorganic matters production organic substance, cell is built, is consumer, decomposer and storage person again, is made thing in ecosystem
The main pusher that matter is circulated and energy is changed, for the reparation of aquiculture pond environment plays very important effect.This
Outward, the metabolite and cell component of many microorganisms has special nutritional labeling and physiological function, can promote fish and shrimp and
Algae, the growth promoter of water plant；Part beneficial microbe can also suppress the breeding of pathogen, reduce sending out for fish and shrimp diseases
It is raw.Thus, we can make full use of the microorganism in pond, increase beneficial microbe in water body by the change of environmental condition
Relative populations, so as to improve water quality, promote healthy growth, the development of cultivated animals, promote the sustainable development of culture fishery
Exhibition, application of the modern microbial ecological agent on aquaculture grow up in this rationale.Kozasa
(1986) microbial ecological agent is applied to into aquaculture first.Aquatile is closely related with water environment, in many cases can be straight
Addition beneficial microbe is connect in breeding water body, cultivated animals is had by antagonism cause of disease, the unnecessary organic matter of degraded etc.
The impact of benefit.Therefore Moriarty (1998) the implication of microbial ecological agent expand to a class be added to it is beneficial in breeding water body
Microorganism.
It is to include Bacillus licheniformis that the microorganism that uses and its microbial ecological agent are widely used at present on aquaculture
In interior bacillus cereuss (Bacillus sp.) etc..Bacillus licheniformis are a kind of bacillus cereuss of homofermentative lactic, and leather is blue
Family name is positive, and lactic acid producing, catalase are positive, and phenylalanine deaminase negative can form spore.Bacillus licheniformis
Can the various saccharides such as starch-splitting, sucrose, arabinose, xylose and Lactose, maltose, decomposition glucose produces acid and do not produce
Gas.Due to the characteristic that there are lactic acid bacteria and bacillus cereuss simultaneously, acid-resistant and anti-high-temperature, with abundant extracellular enzyme, and thalline sheet
Body is nontoxic, therefore in the purification of culture fishery pond water quality has obtained certain application, can significantly reduce pond waters
The content of middle ammonia nitrogen, nitrite nitrogen, hydrogen sulfide and solubility total phosphorus.But current research concentrates on free lichens spore
Bacillus, as which is easily washed away by current under discharge condition, is easily ingested by other biological under hydrostatic condition, and free lichens
Bacillus cereuss cannot natural subsidence to cultivating pool water body water-deposit interface, limit its repair bottom material of pond for breeding energy
Power.Actually this has become a key factor for affecting Bacillus licheniformis application effect.
As bottom material of pond for breeding serves not only as the storage vault of cultivation water holding pond, various chemical substances, or plant, animal and
The habitat of microorganism and nutrient recirculation center, so bottom material of pond for breeding can consumingly affect water with the interaction of water
Matter, therefore want to support good water, just must set about from bottom material of pond for breeding improvement, it is necessary to fully pay attention to the management of bottom material of pond for breeding.Kieselguhr
It is a kind of natural silicate mineral, there is abundant mining deposits in China, with the characteristic and price of adsorbing pollutant
It is cheap.
The content of the invention
Present invention aims to deficiency of the prior art, there is provided a kind of with low cost, easy to operate and can be real
The microorganism water treatment agent in culturing water of existing large-scale production, it is intended to after uniform input cultivating pool, bottom material of pond for breeding can be fallen to, only
Change and repair cultivating pool water-deposit interface pollutant.
A kind of microorganism water treatment agent in culturing water, the microorganism water treatment agent in culturing water are to be fixed with lichens spore
The activated diatomaceous earth granule of bacillus.
Further, the activated diatomaceous earth granule is obtained by following methods：Kieselguhr is stirred in distilled water
After be standing and soak for 24h so as to suspension is made in the dispersion in water, stands, settle 24h after crossing 300 mesh sieves, extract 10 under liquid level～
Suspension more than 15cm depth, obtains activated diatomaceous earth of the particle diameter less than 0.5 μm after centrifugal drying.
Present invention also offers the preparation method of above-mentioned microorganism water treatment agent in culturing water, it is characterised in that including following
Step：
A. 24h is soaked during stand kieselguhr after distilled water stirs so as to which suspension, mistake are made in the dispersion in water
Stand after 300 mesh sieves, settle 24h, extract suspension more than 10～15cm depth under liquid level, particle diameter is obtained after centrifugal drying little
In 0.5 μm of activated diatomaceous earth；
B. by the Bacillus licheniformis liquid and activated diatomaceous earth of exponential phase with volume mass ratio as 1:1～2 ratio
Example, under aerobic conditions mixing are co-cultured；Cultivation temperature is 30～35 DEG C, and the culture increment time is 24-48h, after room temperature is air-dried
The activated diatomaceous earth granule of Bacillus licheniformis must be fixed with, be preserved under room temperature.Described room temperature is 20-28 DEG C.
Microorganism water treatment agent in culturing water of the present invention needs the water-deposition for being equably sprinkling upon cultivating pool water body when in use
Thing interface, according to the different breeding phase, usage amount is 50～100g/m
Microorganism water treatment agent in culturing water of the present invention adopts activated diatomaceous earth as fixation support, with lichens spore bar
Bacterium is immobilization object, and by rising in value with culture, immobilization bacterium amount is big, and bioaccumulation efficiency is high；Bacillus licheniformis are Jing after immobilization
Higher activity not only can be still kept, also other biological can be protected from by activated diatomaceous earth and be ingested, and can sink to rapidly pond
Pool bottom water-deposit interface plays a role and purifies water, and not easily runs off, extends the repair cycle；Silicon after activation
Diatomaceous earth has bigger absorption property, can be with the pollutant of more efficient absorption water-deposit interface, beneficial to the ground after fixation
Degraded of the clothing bacillus cereuss to pollutant, improves the functioning efficiency of absorption degradation pollutant；The whole nontoxic nothing of material used
Evil, non-secondary pollution, and it is with low cost, it is readily transported and stores, is capable of achieving large-scale production.
Specific embodiment
With reference to specific embodiment, the present invention is described in further detail.Embodiments of the invention are using activation
Kieselguhr fix Bacillus licheniformis, and then repair cultivating pool water-deposit interface pollutant.
Embodiment 1
The preparation of activated diatomaceous earth：
According to kieselguhr:Distilled water=1:10 weight ratio is standing and soak for after kieselguhr is stirred in distilled water
24h so as to which suspension is made in the dispersion in water.Stand after crossing 300 mesh sieves, settle 24h, under extraction liquid level more than 15cm depth
Suspension, obtains activated diatomaceous earth of the particle diameter less than 0.5 μm after centrifugal drying.
According to exponential phase Bacillus licheniformis liquid: activated diatomaceous earth=1:The ratio of 2 (volume mass ratios), is needing
It is mixed under the conditions of oxygen, 30～35 DEG C of culture 24h, room temperature are fixed the kieselguhr of Bacillus licheniformis after air-drying
Grain, room temperature preservation.
At the prawn culturing initial stage according to 100g/m2Usage amount, above-mentioned water purification agent is equably sprinkling upon into prawn culturing pond,
Water purification agent can sink to the water-deposit interface of bottom of pond, reach the purpose in purifying aquaculture pond.
Experiment is shown, is compared with the Bacillus licheniformis of addition free state, added activated kieselguhr and fix lichens spore
The water of bacillus experimental group pond bottom of pond-deposit interface ammonia-nitrogen content have dropped 41.9%, and nitrite nitrogen content have dropped
2.3%, hydrogen sulfide content have dropped 22.5%, and total phosphorus content have dropped 36.7%, the content of organic carbon and organic nitrogen respectively under
Drop 27.6% and 24.7%.
Embodiment 2
The preparation of activated diatomaceous earth：
According to kieselguhr:Distilled water=1:20 weight ratio is standing and soak for after kieselguhr is stirred in distilled water
24h so as to which suspension is made in the dispersion in water.Stand after crossing 300 mesh sieves, settle 24h, extract the suspension of more than 10cm depth
Liquid, obtains activated diatomaceous earth of the particle diameter less than 0.5 μm after centrifugal drying.
According to exponential phase Bacillus licheniformis liquid:Activated diatomaceous earth=1:The ratio of 1 (volume mass ratio), is needing
It is mixed under the conditions of oxygen, 30～35 DEG C of culture 36h, room temperature are fixed the kieselguhr of Bacillus licheniformis after air-drying
Grain, room temperature preservation.
In the prawn culturing later stage according to 75g/m2Usage amount, above-mentioned water purification agent is equably sprinkling upon into prawn culturing pond,
Water purification agent can sink to the water-deposit interface of bottom of pond, reach the purpose in purifying aquaculture pond.
Experiment is shown, is compared with the Bacillus licheniformis of addition free state, added activated kieselguhr and fix lichens spore
The water of bacillus experimental group pond bottom of pond-deposit interface ammonia-nitrogen content have dropped 42.8%, and nitrite nitrogen content declines
6.7%, hydrogen sulfide content have dropped 29.4%, and total phosphorus content have dropped 51.6%, the content of organic carbon and organic nitrogen respectively under
Drop 31.2% and 43.8%.
Embodiment 3
The preparation of activated diatomaceous earth：
According to kieselguhr: distilled water=1: 15 weight ratio is standing and soak for after kieselguhr is stirred in distilled water
30h so as to which suspension is made in the dispersion in water.Stand after crossing 200 mesh sieves, settle 24h, extract the suspension of more than 15cm depth
Liquid, obtains activated diatomaceous earth of the particle diameter less than 0.5 μm after centrifugal drying.
According to exponential phase Bacillus licheniformis liquid: activated diatomaceous earth=1: the ratio of 2 (volume mass ratios), needing
It is mixed under the conditions of oxygen, 30～35 DEG C of culture 36h, room temperature are fixed the kieselguhr of Bacillus licheniformis after air-drying
Grain, room temperature preservation.
According to 50g/m during breeding soft-shell turtle2Usage amount, above-mentioned water purification agent is equably sprinkling upon into breeding soft-shell turtle pond,
Water purification agent can sink to the water-deposit interface of bottom of pond, reach the purpose in purifying aquaculture pond.
Experiment is shown, is compared with the Bacillus licheniformis of addition free state, added activated kieselguhr and fix lichens spore
The water of bacillus experimental group pond bottom of pond-deposit interface ammonia-nitrogen content have dropped 21.9%, and nitrite nitrogen content have dropped
2.5%, hydrogen sulfide content have dropped 20.1%, and total phosphorus content have dropped 35.8%, the content of organic carbon and organic nitrogen respectively under
Drop 25.7% and 28.8%.
Embodiment 4
The preparation of activated diatomaceous earth：
According to kieselguhr: distilled water=1: 20 weight ratio is standing and soak for after kieselguhr is stirred in distilled water
36h so as to which suspension is made in the dispersion in water.Stand after crossing 300 mesh sieves, settle 24h, extract the suspension of more than 15cm depth
Liquid, obtains activated diatomaceous earth of the particle diameter less than 0.5 μm after centrifugal drying.
According to exponential phase Bacillus licheniformis liquid: activated diatomaceous earth=1: the ratio of 1 (volume mass ratio), needing
It is mixed under the conditions of oxygen, 30～35 DEG C of culture 48h, room temperature are fixed the kieselguhr of Bacillus licheniformis after air-drying
Grain, room temperature preservation.
According to 50g/m during Carassius aurutus gibelio is cultivated2Usage amount, above-mentioned water purification agent is equably sprinkling upon into Carassius aurutus gibelio and is supported
Pond is grown, water purification agent can sink to the water-deposit interface of bottom of pond, reach the purpose in purifying aquaculture pond.
Experiment is shown, is compared with the Bacillus licheniformis of addition free state, added activated kieselguhr and fix lichens spore
The water of bacillus experimental group pond bottom of pond-deposit interface ammonia-nitrogen content have dropped 28.5%, and nitrite nitrogen content have dropped
9.2%, hydrogen sulfide content have dropped 33.2%, and total phosphorus content have dropped 32.7%, the content of organic carbon and organic nitrogen respectively under
Drop 30.9% and 35.5%.
Finally, in addition it is also necessary to it is noted that listed above is only several specific embodiments of the invention.Obviously, this
It is bright to be not limited to above example, there can also be many deformations.One of ordinary skill in the art can be from present disclosure
The all deformations directly derived or associate, are considered as protection scope of the present invention.
Claims (3)
1. a kind of microorganism water treatment agent in culturing water, it is characterised in that the microorganism water treatment agent in culturing water is to fix
There is the activated diatomaceous earth granule of Bacillus licheniformis.
2. microorganism water treatment agent in culturing water as claimed in claim 1, it is characterised in that the activated diatomaceous earth granule by
Following methods are obtained：24h is standing and soak for after kieselguhr is stirred in distilled water so as to which suspension is made in the dispersion in water
Liquid, stands after crossing 300 mesh sieves, settles 24h, extracts suspension more than 10～15cm depth under liquid level, grain is obtained after centrifugal drying
Activated diatomaceous earth granule of the footpath less than 0.5 μm.
3. a kind of method for preparing microorganism water treatment agent in culturing water as claimed in claim 1, it is characterised in that including following
Step：
A. 24h is standing and soak for after stir kieselguhr in distilled water so as to which suspension is made in the dispersion in water, crosses 300
Stand after mesh sieve, settle 24h, extract suspension more than 10～15cm depth under liquid level, particle diameter is obtained after centrifugal drying and is less than
0.5 μm of activated diatomaceous earth granule；
B. by the Bacillus licheniformis liquid and activated diatomaceous earth granule of exponential phase with volume mass ratio as 1:(1～2)
Ratio, under aerobic conditions mixing are co-cultured；Cultivation temperature be 30～35 DEG C, the culture increment time be 24-48h, 20-28 DEG C of wind
The activated diatomaceous earth granule of Bacillus licheniformis must be fixed with after dry, be preserved at 20-28 DEG C.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107673480A (en) * | 2017-09-19 | 2018-02-09 | 常州市海若纺织品有限公司 | A kind of preparation method of cycle of sixty years shell substrate modifier |
| CN108587916A (en) * | 2018-05-23 | 2018-09-28 | 昆明理工大学 | A method of co-culturing single needle algae rapid flocculation in neutral conditions |
| WO2018190772A1 (en) * | 2017-04-12 | 2018-10-18 | Blue Aqua International Pte. Ltd. | Composition for aquaculture |
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| CN101811775A (en) * | 2009-02-19 | 2010-08-25 | 中国环境科学研究院 | Composite bacteria-algae preparation method for purifying aquaculture waste water |
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