CN106277320A - A kind of Penaeus vannamei freshwater cultivation water regulation method - Google Patents

A kind of Penaeus vannamei freshwater cultivation water regulation method Download PDF

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CN106277320A
CN106277320A CN201610774674.6A CN201610774674A CN106277320A CN 106277320 A CN106277320 A CN 106277320A CN 201610774674 A CN201610774674 A CN 201610774674A CN 106277320 A CN106277320 A CN 106277320A
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water
photosynthetic bacteria
outlet
microdisk electrode
cultivation
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CN106277320B (en
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陈加雄
李伟滨
马庆涛
陈伟洲
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Shantou Marine Plant Research Station Of Chinese Academy Of Sciences
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Shantou Marine Plant Research Station Of Chinese Academy Of Sciences
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/30Aerobic and anaerobic processes
    • C02F3/302Nitrification and denitrification treatment
    • C02F3/303Nitrification and denitrification treatment characterised by the nitrification
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/32Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • C02F3/322Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
    • C02F3/325Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/348Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/10Inorganic compounds
    • C02F2101/105Phosphorus compounds
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/10Inorganic compounds
    • C02F2101/16Nitrogen compounds, e.g. ammonia
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/30Organic compounds
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/20Nature of the water, waste water, sewage or sludge to be treated from animal husbandry

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Water Supply & Treatment (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Ecology (AREA)
  • Botany (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

A kind of Penaeus vannamei freshwater cultivation water regulation method, comprises the steps: (1) anaerobic nitrification successively;(2) aerobic nitrification;(3) Treatment of Psb;(4) microdisk electrode purifies.The water extracted out from the freshwater of cultivation Penaeus vannamei, successively after anaerobic nitrification, aerobic nitrification, Treatment of Psb and microdisk electrode purify, flows back in the freshwater of cultivation Penaeus vannamei.Organic substance in water can be resolved into small-molecule substance by the present invention, small-molecule substance is absorbed by photosynthetic bacteria and the microdisk electrode supported of holding concurrently, it is transformed into microalgae organism, treated qualified water is discharged into pond circulation together with the useful microalgae cultivated and utilizes, useful microalgae forms population advantage after entering pond, improve breeding ecological environment, water treatment effect is good, and whole water treatment procedure departs from breeding water body, do not affect the normal growth of prawn, the method is workable, and operating cost is cheap, and the Penaeus vannamei for cultivation is safe and reliable.

Description

A kind of Penaeus vannamei freshwater cultivation water regulation method
Technical field
The present invention relates to technical field of aquaculture, be specifically related to a kind of Penaeus vannamei freshwater cultivation water regulation Method.
Background technology
Water is Penaeus vannamei existence, the environmental condition of growth, and the quality of water quality directly affects cultured output.How to regulate Good water quality, promotes the quick healthy growth of Penaeus vannamei, is the problem that always annoying raiser.In whole breeding process, Build emphasis and difficult point that good and stable water quality is shrimp raising technology.
Culture of Penaeus vannamei is the process of " pollution ", and at the middle and late stage of pond culture, prawn mainly ingests manually Mixed feed, the Excreta of residual bait and prawn constantly accumulates, and animals and plants corpse is continuously increased, these residual feed and excretas, animals and plants Corpse is deposited at the bottom of pond and carries out anaerobic decomposition, produces substantial amounts of harmful substance, ruins water quality, substrate, causes the evil of breeding environment Change, mainly show themselves in that 1, the substantial amounts of oxygen of biological oxidation decomposition and consumption at the bottom of pond, make prawn be in the environment of hypoxia, its body constitution and Resistance against diseases declines;2, too much nutrition residue, adds the environment of hypoxia, makes substantial amounts of facultative anaerobe decomposing organic matter matter, Generate a large amount of intermediate product such as ammonia nitrogen, nitrite, hydrogen sulfide, organic acid, prawn is caused serious toxic action;3, bed mud Too much nutrition release, causes body eutrophication, and meanwhile, bottom is because acidifying, and the micronutrient such as calcium, magnesium, ferrum, manganese is with insoluble The form of property salt is deposited on bed mud and is difficult to discharge, and causes nutrient imbalance, and the growth of useful algae is suppressed, and likes eutrophic indigo plant Algae, dinoflagellate etc. become dominant population, water quality deterioration;4, the environment of hypoxia height nutrition makes the pathogenic bacterium Fast-propagations such as vibrio, makes Prawn occurs that disease is the most dead.
At present, prawn culturing regulating and controlling water quality purpose is to reduce the Organic substance of water body, and then reduces BOD and pond Trophic level.The raising of dissolved oxygen can make the breeding of the anaerobe such as vibrio be suppressed, and decreases reproducibility simultaneously and poisons thing The accumulation of matter, suppression cyanophyceae and the growth of useful algae such as the growth of dinoflagellate, beneficially chlorella and diatom etc., stablize bacterium phase and algae phase, Improve prawn premunition.
Existing Penaeus vannamei pond culture water quality regulation and control method mainly has Physical, chemical method and bioanalysis three major types Regulating and controlling water quality method, the most conventional is bioanalysis, but these methods are all because processing cost is high, success rate is low, can grasp The property made difference or effect are the most notable.
Penaeus vannamei pond culture water quality regulation and control method is most commonly used that phycomycete control technique, by planktonic microalgae and The common effect of beneficial bacteria, Degradation and Transformation cultivation metabolite and residual bait, regulating water quality, suppression noxious bacteria and harmful microalgae Grow, build suitable prawn growth ecological environment.Main technical schemes is: select sun-drenched weather, throws to pond Put that dissolubility is good, the suitable inorganic composite nutrition of proportioning, organic composite nutrient and trace element, cultivate useful microalgae;In cultivation During periodically uniformly throw in bacillus cereus to pond, throw in photosynthetic bacteria and lactic acid bacteria etc. depending on water quality situation, keep probiotics The advantage Ecological niche in pond;Use " molasses " supplementary carbon source at cultivation middle and late stage, promote that " bacterium phase " and " algae phase " is stable.So And, phycomycete control technique less stable, and commonly raiser is difficult to grasp;The cooperation nutrient thrown in pond during training algae, Major part is agrochemical, is difficult to uniformly apply, and only small part is utilized by algae, and major part nutrition is absorbed by bed mud, can make At the bottom of pond, organic matter is more, causes substrate to deteriorate, easily causes the harmful algae excessive propagations such as cyanophyceae;Microalgae population knot in pond Structure is susceptible to change along with the change of environmental condition, and " algae phase " is unstable;Beneficial bacteria and photosynthetic bacteria is put in pond Time, its Organic substance to be degraded is predominantly located in pond bottom, and is distributed and uneven, and mushroom can not spread with a definite target in view To target substrates, waste resource;Genus lactubacillus is in anaerobe, and on water body upper strata, especially live in oxygen-rich area near aerator etc. Property is substantially reduced;And photosynthetic bacteria lacks decomposition substrate when the upper strata of water, again because of insufficient light at the bottom of pond close to substrate time And do not have effect;Additionally some microorganism such as bacillus cereus oxygen consumption in nitrifying process is serious, directly can consume in pond Dissolved oxygen in water body, is unfavorable for the growth of prawn.
Summary of the invention
The technical problem to be solved is to provide a kind of Penaeus vannamei freshwater cultivation water regulation method, Organic substance in water can be resolved into small-molecule substance by the method, and small-molecule substance is trained by photosynthetic bacteria and the microalgae supported of holding concurrently Supporting and absorb, be transformed into microalgae organism, treated qualified water is discharged into pond circulation profit together with the useful microalgae cultivated With, useful microalgae forms population advantage after entering pond, improves breeding ecological environment, and water treatment effect is good, and whole water Processing procedure departs from breeding water body, does not affect the normal growth of prawn, and the method is workable, and operating cost is cheap, for The Penaeus vannamei of cultivation is safe and reliable.The technical scheme used is as follows:
A kind of Penaeus vannamei freshwater cultivation water regulation method, it is characterised in that comprise the steps: successively
(1) anaerobic nitrification
Anaerobic nitrification reaction is carried out in anaerobic tank;
Utilizing water pumper that the water in the freshwater of cultivation Penaeus vannamei is pumped into anaerobic tank, Organic substance contained in water is being changed Manure pit precipitates, forms natural activity mud, carry out anaerobic nitrification;
(2) aerobic nitrification
Aerobic nitrification reaction is carried out in open Aeration tank, and this open Aeration tank is passed through pipeline string by two Aeration tank unit Connection forms, and first Aeration tank unit bottom lays air aeration head, and first Aeration tank unit is provided with Aeration tank water inlet Mouthful, second Aeration tank unit is provided with Aeration tank outlet, and Aeration tank water inlet connects with the outlet of anaerobic tank, Aeration tank Outlet position is less than the outlet of anaerobic tank;
1 bacillus cereus of interpolation in Aeration tank per week, add 100 milliliters (purpose adding bacillus cereus is to protect every time Card aerobic nitrification effect);
(3) Treatment of Psb
Treatment of Psb process is carried out in photosynthetic bacteria reactor, and photosynthetic bacteria reactor includes at least one photosynthetic bacteria Reactor unit, photosynthetic bacteria reactor unit includes that the vessel that clear glass is made, vessel upper end open are added a cover There is the container cover being made up of light red glass;The water inlet of photosynthetic bacteria reactor connects with Aeration tank outlet, photosynthetic bacteria The outlet position of reactor is less than Aeration tank outlet;
1 photosynthetic bacteria (such as rhodospirillum) of interpolation in vessel per week, adds 100 milliliters, to ensure at container every time In body, photosynthetic bacteria becomes dominant microflora, detergent power of increasing water quality;
(4) microdisk electrode purifies
Microdisk electrode purification process is carried out in open microdisk electrode pond, and this microdisk electrode pond is by multiple microdisk electrode pool units Being formed by placed in series, first microdisk electrode pool unit is provided with microdisk electrode pond water inlet, last microdisk electrode Pool unit is provided with microdisk electrode pond outlet, and microdisk electrode pond water inlet connects with the outlet of photosynthetic bacteria reactor, micro- Algae culture pond outlet connects with the freshwater of cultivation Penaeus vannamei, and outlet position, microdisk electrode pond is less than photosynthetic bacteria The outlet of reactor;
Microdisk electrode pond is thrown in and has microalgae, microalgae one in little ring algae, Skeletonema Costatum, chlorella and scenedesmus or its In multiple combination.
After step (1) anaerobic nitrification and step (2) aerobic nitrification, the larger molecular organics in water has decomposed Become small organic molecule;In step (3), photosynthetic bacteria is under anaerobism illumination condition, it is possible to utilize these small organic molecules Carrying out auto-trophy growth as photosynthetic electron acceptor, the small organic molecule in water is absorbed, thus realizes water Purification;Water body after step (3) processes contains the inorganic substances such as substantial amounts of carbon, nitrogen, phosphorus, as micro-in step (4) The photosynthetic nutrition of algae, is absorbed during micro algae growth, and ammonia nitrogen assimilates, and the oxygen environment in microdisk electrode pond also presses down Having made nitrite generation, small organic molecule is absorbed that (nutrient substance in water body can promote the most again by microalgae heterotrophic growth Enter micro algae growth breeding), realize water purification further, realize the cultivation of useful microalgae simultaneously.Water after treatment is even It is back to cultivate the freshwater of Penaeus vannamei together with the useful microalgae cultivated.
The outlet of above-mentioned anaerobic tank, Aeration tank outlet, the outlet of photosynthetic bacteria reactor, the water outlet of microdisk electrode pond Mouthful position reduce successively, so, rely on action of gravity can realize water anti-anaerobic tank, open Aeration tank, photosynthetic bacteria Answer between device, microdisk electrode pond natural flow successively.
In preferred steps (1), when anaerobic tank uses first, in anaerobic tank, add initial activity mud;Initial activity is dirty The manufacture method of mud is as follows: take pond wet bed mud 2000-2400 part, prawn feed 600-800 part, sucrose 40-60 by weight Part, starch 40-60 part, Carnis Bovis seu Bubali cream 15-25 part and lactobacillus 180-220 part, be modulated into pastel with water after mix homogeneously, Again pastel put in container (such as conical flask) and container sealed, being subsequently placed in 32 DEG C of incubators cultivation 36 hours, Obtain initial activity mud.The wet bed mud in above-mentioned pond is taken from the bottom of the freshwater pond of cultivation Penaeus vannamei, drains, i.e. after taking-up The available required wet bed mud in pond.Prawn feed uses the prawn feed of market sale.It is white that above-mentioned water takes from cultivation South America The freshwater of prawn.The initial activity mud obtained after cultivation contains and includes zymogenous bacteria, produces hydroacetic acid antibacterial, methanogen Deng a large amount of active anaerobic bacteria floras.Generally, the anaerobic tank of every 1000 liters of capacity adds 2.5-3.5 kilogram of initial activity dirt Mud, the consumption of initial activity mud increases and decreases in proportion according to anaerobic tank amount of capacity.
In preferred steps (1), anaerobic tank changes water 1 time in every 4 hours, and water pumper arranges timing controlled, the every task of water pumper 4 Hour (now anaerobic tank changes water) stops 4 hours (now carrying out anaerobic nitrification in anaerobic tank) afterwards, makes anaerobic nitrification react Entirely.The hydromining that changes of anaerobic tank enters while the mode gone out is carried out, and while water pumper draws water entrance anaerobic tank, anaerobic tank passes through Its outlet water outlet, the open Aeration tank of current direction flowed out from the outlet of anaerobic tank, the current direction light of open Aeration tank Closing bacterial reaction device, the current direction microdisk electrode pond of photosynthetic bacteria reactor, it is white that the water in microdisk electrode pond is back to cultivate South America The freshwater of prawn.When water pumper stops, water is not at anaerobic tank, open Aeration tank, photosynthetic bacteria reactor, microdisk electrode Flowing between pond, now anaerobic tank, open Aeration tank, photosynthetic bacteria reactor, microdisk electrode pond carry out anaerobic nitrification, good respectively Oxygen nitrification, Treatment of Psb, microdisk electrode purify.
In preferred steps (1), water pumper is when the freshwater of cultivation Penaeus vannamei draws water, and intake is positioned at aerator Neighbouring (intake is generally in the range of within 1 meter of aerator), makes to be deposited in the insoluble Organic substance at the bottom of pond along with water body Agitation be suspended in water, be beneficial to water pumper and these Organic substances extracted.
Above-mentioned anaerobic tank can use the three-grid septic tank that capacity is 1000 liters (such as glass toughening manure pit) of market sale, should Anaerobic tank has three the anaerobic tank unit being sequentially communicated from A-P, and water pumper is from the freshwater of cultivation Penaeus vannamei The water of extraction enters the anaerobic tank unit of foremost, and the outlet of anaerobic tank is located at rearmost anaerobic tank unit.
In preferred steps (2), Aeration tank unit cement or ceramic material, in long column type;Each Aeration tank unit Volume is 100 liters, and base plane is 30 cm x 30 centimetres, high 120 centimetres.
First Aeration tank unit bottom lays air aeration head (a usually air aeration head), air aeration head Can oxygen supplement in first Aeration tank unit, make the internal oxygen of first Aeration tank unit sufficient;Second Aeration tank unit does not has Laying air aeration head, second the internal oxygen of Aeration tank unit weakens, and so it is advantageously ensured that step (3) Treatment of Psb institute The low-oxygen environment needed.
In preferred steps (3), photosynthetic bacteria reactor includes two photosynthetic bacteria reactor units, and two photosynthetic bacterias are anti- Answering the vessel of device unit by placed in series, the water inlet of photosynthetic bacteria reactor is located at first photosynthetic bacteria reactor On unit, the outlet of photosynthetic bacteria reactor is located on second photosynthetic bacteria reactor unit.Preferably vessel capacity Being 50 liters, its base plane is 30 cm x 30 centimetres, high 60 centimetres, and the outlet of photosynthetic bacteria reactor can be located at container originally 50 centimetres of eminences of body.
In above-mentioned photosynthetic bacteria reactor, the container cover being made up of light red glass can optionally allow long wave light lead to Cross, to promote photosynthetic bacteria Fast-propagation.
Microdisk electrode pool unit can make by cement or tile material, and each microdisk electrode pool unit area is 0.64 square Rice, height is 50 centimetres;Microdisk electrode pond outlet can be located at 35 centimetres of eminences of last microdisk electrode pool unit, it is ensured that training Support depth of water 35 centimetres.It is relative that the water inlet of each microdisk electrode pool unit is usually provided in microdisk electrode pool unit with outlet Side, extends into Water flow-path and makes water body mixing as far as possible in microdisk electrode pool unit.
Microdisk electrode pond outlet connects through the freshwater of return pipe with cultivation Penaeus vannamei, the end of return pipe Discharge outlet should be away from intake (distance between discharge outlet and intake be generally more than 20 meters), it is to avoid after purified process Water pumpback is to anaerobic tank.
In above-mentioned steps (4), algae kind may utilize the microalgae of growth naturally in cultivation just freshwater in mid-term, chooses therein Beneficial diatoms and the chlorellas such as little ring algae, Skeletonema Costatum, chlorella, scenedesmus, cultivate through isolated and purified expanding species, and by target algae Plant and save backup in indoor cultivation case.
In the present invention, each step is individually completed by different bioreactors, anaerobic tank, open Aeration tank, photosynthetic Bacterial reaction device and microdisk electrode pond front and back connect in order, constitute a complete water treatment system;The most right from cultivation South America The water extracted out in the freshwater of shrimp flows through anaerobic tank, open Aeration tank, photosynthetic bacteria reactor and microdisk electrode pond successively, After carrying out anaerobic nitrification, aerobic nitrification, Treatment of Psb and microdisk electrode purification, flow back into the light of cultivation Penaeus vannamei In the pool, pond.Water quality after treatment reaches the requirement of " water quality standard for fishery " (GB 11607-89), and the most qualified water is even Being back to pond circulation together with the useful microalgae cultivated utilize, useful microalgae forms population advantage after constantly entering pond, has The algae being beneficial to breeding water body is improved mutually, promotes the photosynthesis of planktonic algae, improves the dissolved oxygen concentration of breeding water body, reduces and supports Grow the organic concentration in water body, reduce ammonia nitrogen and content of nitrite, improve breeding ecological environment.And, the present invention detests The various process such as oxygen nitrification, aerobic nitrification, Treatment of Psb and microdisk electrode purification do not interfere with each other, and are used in combination and are connected into One complete processing system, whole processing procedure separates with breeding water body, and the Penaeus vannamei for cultivation does not cause any Infringement, does not affect the normal growth of prawn.
The present invention is workable, and equipment is simple, and cheap, floor space is less, and treatment in accordance with local conditions is installed, water treatment Respond well, operating cost is cheap, has bigger promotional value.
During prawn culturing, if due to residual bait, planktonic metabolite and the excretion of prawn in breeding water body Thing increases and causes the Organic substance accumulation in water body too much, and water quality index occurs abnormal, then must start up regulating and controlling water quality system, carry out Regulating and controlling water quality.
Accompanying drawing explanation
Fig. 1 is the structural representation of the water treatment system used by the preferred embodiment of the present invention.
Detailed description of the invention
With reference to Fig. 1, in the present embodiment, Penaeus vannamei freshwater cultivation water regulation method includes following step successively Rapid:
(1) anaerobic nitrification
Anaerobic nitrification reaction is carried out in anaerobic tank 2;Water pumper 1 is utilized to be taken out by the water in the freshwater of cultivation Penaeus vannamei Evolution manure pit 2(water pumper 1 is when the freshwater of cultivation Penaeus vannamei draws water, and intake is positioned near aerator), in water Contained Organic substance precipitates in anaerobic tank 2, forms natural activity mud, carries out anaerobic nitrification;
The three-grid septic tank (such as glass toughening manure pit) that anaerobic tank 2 uses capacity to be 1000 liters, this anaerobic tank 2 has from A-P Three the anaerobic tank unit 21(being sequentially communicated are followed successively by anaerobic tank unit 21-1,21-2,21-3), water pumper 1 is from cultivation South America In the freshwater of white shrimp, the water of extraction enters the anaerobic tank unit 21-1 of foremost, and the outlet 22 of anaerobic tank 2 is located at Anaerobic tank unit 21-3 below;When this anaerobic tank 2 uses first, in anaerobic tank 2, add 3 kilograms of initial activity mud;
The manufacture method of initial activity mud is as follows: take the wet bed mud in pond 2200 grams, prawn feed 700 grams, sucrose by weight 50 grams, starch 50 grams, Carnis Bovis seu Bubali cream 20 grams and lactobacillus 200 grams, be modulated into pastel with water after mix homogeneously, then by pasty state Thing is put in container (such as 5000 milliliters of conical flasks) and is sealed by container, is subsequently placed in 32 DEG C of incubators cultivation 36 hours, Obtain initial activity mud (taking 3 kilograms from the initial activity mud obtained to add in anaerobic tank 2);
(2) aerobic nitrification
Aerobic nitrification react at the open the most open-topped Aeration tank of Aeration tank 3() in carry out, this open Aeration tank 3 is by two Individual Aeration tank unit 31(is followed successively by Aeration tank unit 31-1,31-2) it is in series by pipeline 32, first Aeration tank unit 31-1 basis rest has an air aeration head 33, first Aeration tank unit 31-1 to be provided with Aeration tank water inlet 35, and second Individual Aeration tank unit 31-2 is provided with Aeration tank outlet 34, and Aeration tank water inlet 35 connects with the outlet 22 of anaerobic tank 2, exposes Outlet 34 position, gas pond is less than the outlet 22 of anaerobic tank 2;
Aeration tank unit 31 cement or ceramic material, in long column type;Each Aeration tank unit 31 volume is 100 liters, the end Facial planes is 30 cm x 30 centimetres, high 120 centimetres;
1 bacillus cereus of interpolation in Aeration tank 3 per week, adds 100 milliliters every time;
(3) Treatment of Psb
Treatment of Psb process is carried out in photosynthetic bacteria reactor 4;Photosynthetic bacteria reactor 4 includes that two photosynthetic bacterias are anti- Device unit 41(is answered to be followed successively by photosynthetic bacteria reactor unit 41-1,41-2), photosynthetic bacteria reactor unit 41 includes transparent glass The vessel 411 that glass is made, vessel 411 upper end open adds and is stamped the container cover 412 being made up of light red glass;Two The vessel 411 of photosynthetic bacteria reactor unit 41 is connected by pipeline 44, and the water inlet 42 of photosynthetic bacteria reactor 4 is located at On first photosynthetic bacteria reactor unit 41-1, it is anti-that the outlet 43 of photosynthetic bacteria reactor 4 is located at second photosynthetic bacteria Answer on device unit 41-2;The water inlet 42 of photosynthetic bacteria reactor 4 connects with Aeration tank outlet 34, photosynthetic bacteria reactor 4 Outlet 43 position less than Aeration tank outlet 34;
Each vessel capacity is 50 liters, and its base plane is 30 cm x 30 centimetres, high 60 centimetres, photosynthetic bacteria reactor Outlet can be located at 50 centimetres of eminences of vessel;
1 photosynthetic bacteria (such as rhodospirillum) of interpolation in vessel per week, adds 100 milliliters every time;
(4) microdisk electrode purifies
Microdisk electrode purification process is carried out in open microdisk electrode pond 5, this microdisk electrode pond 5 by multiple (such as six, Fig. 1 In only illustrate first and last) microdisk electrode pool unit 51 formed by placed in series, first microdisk electrode pond is single Unit 51 is provided with microdisk electrode pond water inlet 52, and last microdisk electrode pool unit 51 is provided with microdisk electrode pond outlet 53, microdisk electrode pond water inlet 52 connects with the outlet 43 of photosynthetic bacteria reactor 4, and microdisk electrode pond outlet 53 passes through back Water pipe 6 connects with the freshwater of cultivation Penaeus vannamei, and outlet 53 position, microdisk electrode pond is less than photosynthetic bacteria reactor 4 Outlet 43;
Microdisk electrode pool unit 51 cement or tile material make, and each microdisk electrode pool unit 51 area is 0.64 square Rice, height is 50 centimetres;Microdisk electrode pond outlet 53 is located at 35 centimetres of eminences of last microdisk electrode pool unit, it is ensured that training Support depth of water 35 centimetres;
Microdisk electrode pond 5 is thrown in have microalgae (microalgae one in little ring algae, Skeletonema Costatum, chlorella and scenedesmus or Many of combination).
Algae kind used by step (4) may utilize the microalgae of growth naturally in cultivation just freshwater in mid-term, chooses therein Beneficial diatoms and the chlorellas such as little ring algae, Skeletonema Costatum, chlorella, scenedesmus, cultivate through isolated and purified expanding species, and by target algae Plant and save backup in indoor cultivation case.
Microdisk electrode pond outlet 53 connects through the freshwater of return pipe 6 with cultivation Penaeus vannamei, the end of return pipe 6 The discharge outlet of end should be away from intake, it is to avoid the water pumpback after purified process is to anaerobic tank.
The outlet 22 of anaerobic tank 2, Aeration tank outlet 34, the outlet 43 of photosynthetic bacteria reactor 4, microdisk electrode pond The position of outlet 53 reduces successively, so, relies on action of gravity can realize water at anaerobic tank 2, open Aeration tank 3, light Close natural flow successively between bacterial reaction device 4, microdisk electrode pond 5.
When startup regulating and controlling water quality system carries out regulating and controlling water quality, anaerobic tank 2 changes water 1 time in every 4 hours, and water pumper 1 arranges timing Controlling, water pumper 1 every task 4 hours (now anaerobic tank 2 changes water) stops (now carrying out anaerobism nitre in anaerobic tank 2 in 4 hours afterwards Change).The hydromining that changes of anaerobic tank 2 enters while the mode gone out is carried out, while water pumper 1 draws water entrance anaerobic tank 2, and anaerobic tank 2 pass through its outlet water outlet, the open Aeration tank of current direction 3 flowed out from the outlet 22 of anaerobic tank 2, open Aeration tank 3 Current direction photosynthetic bacteria reactor 4, the current direction microdisk electrode pond 5 of photosynthetic bacteria reactor 4, the water in microdisk electrode pond 5 returns Flow to cultivate the freshwater of Penaeus vannamei.When water pumper 1 stops, water not anaerobic tank 2, open Aeration tank 3, photosynthetic carefully Flow between bacterium reactor 4, microdisk electrode pond 5, now anaerobic tank 2, open Aeration tank 3, photosynthetic bacteria reactor 4, microalgae training Support pond 5 and carry out anaerobic nitrification, aerobic nitrification, Treatment of Psb, microdisk electrode purification respectively.
After step (1) anaerobic nitrification and step (2) aerobic nitrification, the larger molecular organics in water has decomposed Become small organic molecule;In step (3), photosynthetic bacteria is under anaerobism illumination condition, it is possible to utilize these small organic molecules Carrying out auto-trophy growth as photosynthetic electron acceptor, the small organic molecule in water is absorbed, thus realizes water Purification;Water body after step (3) processes contains the inorganic substances such as substantial amounts of carbon, nitrogen, phosphorus, as micro-in step (4) The photosynthetic nutrition of algae, is absorbed during micro algae growth, and ammonia nitrogen assimilates, and the oxygen environment in microdisk electrode pond also presses down Having made nitrite generation, small organic molecule is absorbed by microalgae heterotrophic growth the most again, realizes water purification further, Realize the cultivation of useful microalgae simultaneously.It is the most right that water after treatment is back to cultivate South America together with the useful microalgae cultivated The freshwater of shrimp, it is achieved recycling and water correction of water.

Claims (7)

1. a Penaeus vannamei freshwater cultivation water regulation method, it is characterised in that comprise the steps: successively
(1) anaerobic nitrification
Anaerobic nitrification reaction is carried out in anaerobic tank;
Utilizing water pumper that the water in the freshwater of cultivation Penaeus vannamei is pumped into anaerobic tank, Organic substance contained in water is being changed Manure pit precipitates, forms natural activity mud, carry out anaerobic nitrification;
(2) aerobic nitrification
Aerobic nitrification reaction is carried out in open Aeration tank, and this open Aeration tank is passed through pipeline string by two Aeration tank unit Connection forms, and first Aeration tank unit bottom lays air aeration head, and first Aeration tank unit is provided with Aeration tank water inlet Mouthful, second Aeration tank unit is provided with Aeration tank outlet, and Aeration tank water inlet connects with the outlet of anaerobic tank, Aeration tank Outlet position is less than the outlet of anaerobic tank;
1 bacillus cereus of interpolation in Aeration tank per week, adds 100 milliliters every time;
(3) Treatment of Psb
Treatment of Psb process is carried out in photosynthetic bacteria reactor, and photosynthetic bacteria reactor includes at least one photosynthetic bacteria Reactor unit, photosynthetic bacteria reactor unit includes that the vessel that clear glass is made, vessel upper end open are added a cover There is the container cover being made up of light red glass;The water inlet of photosynthetic bacteria reactor connects with Aeration tank outlet, photosynthetic bacteria The outlet position of reactor is less than Aeration tank outlet;
1 photosynthetic bacteria of interpolation in vessel per week, adds 100 milliliters every time;
(4) microdisk electrode purifies
Microdisk electrode purification process is carried out in open microdisk electrode pond, and this microdisk electrode pond is by multiple microdisk electrode pool units Being formed by placed in series, first microdisk electrode pool unit is provided with microdisk electrode pond water inlet, last microdisk electrode Pool unit is provided with microdisk electrode pond outlet, and microdisk electrode pond water inlet connects with the outlet of photosynthetic bacteria reactor, micro- Algae culture pond outlet connects with the freshwater of cultivation Penaeus vannamei, and outlet position, microdisk electrode pond is less than photosynthetic bacteria The outlet of reactor;
Microdisk electrode pond is thrown in and has microalgae, microalgae one in little ring algae, Skeletonema Costatum, chlorella and scenedesmus or its In multiple combination.
Penaeus vannamei freshwater cultivation water regulation method the most according to claim 1, it is characterised in that: step (1), in, when anaerobic tank uses first, in anaerobic tank, initial activity mud is added;The manufacture method of initial activity mud is as follows: Take pond wet bed mud 2000-2400 part, prawn feed 600-800 part, sucrose 40-60 part, starch 40-60 part, cattle by weight Meat extract 15-25 part and lactobacillus 180-220 part, be modulated into pastel with water after mix homogeneously, then pastel put into appearance Seal in device and by container, be subsequently placed in 32 DEG C of incubators cultivation 36 hours, obtain initial activity mud.
Penaeus vannamei freshwater cultivation water regulation method the most according to claim 2, it is characterised in that: every 1000 The anaerobic tank rising capacity adds 2.5-3.5 kilogram of initial activity mud.
Penaeus vannamei freshwater cultivation water regulation method the most according to claim 1, it is characterised in that: anaerobic tank Within every 4 hours, changing water 1 time, water pumper arranges timing controlled, and the every task of water pumper stopped 4 hours after 4 hours.
Penaeus vannamei freshwater cultivation water regulation method the most according to claim 1, it is characterised in that: step (1), in, water pumper is when the freshwater of cultivation Penaeus vannamei draws water, and intake is positioned near aerator.
Penaeus vannamei freshwater cultivation water regulation method the most according to claim 1, it is characterised in that: step (3) in, photosynthetic bacteria reactor includes two photosynthetic bacteria reactor units, and the container of two photosynthetic bacteria reactor units is originally Body passes through placed in series, and the water inlet of photosynthetic bacteria reactor is located on first photosynthetic bacteria reactor unit, photosynthetic bacteria The outlet of reactor is located on second photosynthetic bacteria reactor unit.
Penaeus vannamei freshwater cultivation water regulation method the most according to claim 1 or 5, it is characterised in that: micro- Algae culture pond outlet through return pipe with cultivation Penaeus vannamei freshwater connect, the discharge outlet of the end of return pipe away from Intake.
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CN112723543A (en) * 2020-12-11 2021-04-30 浙江大学 Floating type multifunctional water quality purification device and application method thereof
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CN114988653A (en) * 2022-07-19 2022-09-02 中国科学院水生生物研究所 Aquaculture system and method for improving utilization rate of nitrogen and phosphorus and reducing emission of nitrogen and phosphorus
CN116584429A (en) * 2023-04-19 2023-08-15 武汉轻工大学 Industrial freshwater shrimp culturing system

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