CN113860512B - Indigenous algicidal bacterium activating agent and method for preventing cyanobacterial bloom by adopting same - Google Patents
Indigenous algicidal bacterium activating agent and method for preventing cyanobacterial bloom by adopting same Download PDFInfo
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- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 10
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 10
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- 239000001110 calcium chloride Substances 0.000 claims abstract description 10
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 10
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims abstract description 10
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- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims abstract description 6
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 claims abstract description 6
- 239000013078 crystal Substances 0.000 claims abstract description 4
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- 238000003756 stirring Methods 0.000 claims description 24
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- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 13
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 6
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- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 claims description 5
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- 235000005822 corn Nutrition 0.000 claims description 4
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- 239000004695 Polyether sulfone Substances 0.000 claims description 3
- 239000000178 monomer Substances 0.000 claims description 3
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- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 3
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- 239000004814 polyurethane Substances 0.000 claims description 3
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/14—Additives which dissolves or releases substances when predefined environmental conditions are reached, e.g. pH or temperature
Abstract
The invention provides an indigenous algicidal bacteria activating agent and a method for preventing cyanobacterial bloom by adopting the same, wherein the composite agent comprises polyvinyl alcohol, sodium alginate, calcium chloride and composite microorganism according to the mass ratio of 550:35:12:3, and comprises the following components: ferrous salt or ferrous salt crystal water, recoverable waste raw material particles, micro-filtration membrane polymer, potassium persulfate and 5-10 parts of N, N' -dimethylformamide. The composite medicament consisting of the polyvinyl alcohol, the sodium alginate, the calcium chloride and the composite microorganism is loaded on the biomass carbon-nano iron/micro-filtration membrane polymer carrier prepared by self, and the activated medicament can achieve a slow release effect due to the adoption of the biomass carbon-nano iron/micro-filtration membrane polymer carrier prepared by self, so that the activity of the activated medicament is effectively reserved, and the activated medicament is further applied to eutrophic water bodies, and the cyanobacteria bloom phenomenon is effectively prevented.
Description
Technical Field
The invention belongs to the technical field of application of microorganisms to water treatment, and particularly relates to an indigenous algicidal bacterium activating agent and a method for preventing cyanobacterial bloom by adopting the same.
Background
The water resources of China are rich in a plurality of rivers and lakes, algae are excessively bred due to eutrophication, the transparency is improved after black odor is eliminated, the algae burst rate is further accelerated, and the water body is at great risk of black odor recovery. Algae outbreaks caused by eutrophication cause problems of blackness and odor of water bodies, decay of aquatic plants, death of aquatic animals and the like, and serious problems can even cause serious unbalance of the structure and the function of the water body ecological system (Li Rehua and the like, 2015). In addition, the explosion of blue algae can cause the out-of-standard algae toxins in water (Dolah et al, 2015) and seriously threaten the urban water supply safety and the health of residents. Therefore, inhibiting algae outbreaks caused by eutrophication in rivers, lakes and water source areas is a serious water environment problem to be solved urgently in China.
In blue algae treatment works in the past decades, various treatment methods are tried around the world, including physical methods such as mechanical salvage, clay flocculation, nutrition control, aeration mixing, water dilution, hydrodynamic control, and chemical methods such as algicidal agents, electrochemistry, photochemical degradation, and the like. The physical method has high algae treatment effect, but has high energy consumption and high cost, and can not be used in large-area natural lakes and drinking water sources. The chemical method can eliminate blue algae in a short time, but often causes secondary pollution. Biological algae control is an economical and safe algae control technology, and mainly comprises aquatic plant algae control, aquatic animal algae control, microorganism algae control and the like.
The algae control of aquatic animals can be achieved by adopting classical biological or non-classical biological control technology through the filter feeding effect of the aquatic animals (Liu Jiankang, xie Ping, 2003; liu Jigen, 2005), but artificial control of natural enemies of the animals is usually needed, and a good living environment is created for the natural enemies, which is difficult to achieve in most water bodies. The algae control of aquatic plants achieves the aim of inhibiting algae by recovering the submerged plants to compete for nutrient salts or excrete allelochemicals. The technology has been successful in slightly eutrophic water (Sayer et al 2010), but the expected effect is difficult to achieve in water with higher pollution degree. In natural evolution, algae and microorganism form a close synergistic and competitive relationship, and researches show that the sudden disappearance of cyanobacterial bloom is related to bacterial infection, so that the microorganism is gradually changed into a research hotspot as a control measure of cyanobacterial bloom (TAYLOR FJ R, 1968), and the microorganism has great application value as a potential organism for cyanobacterial bloom control.
However, in the prior art, an activation method of indigenous algicidal bacteria capable of inhibiting oxygen-enriched oxidation of a water body is lacking, so that the indigenous algicidal bacteria cannot effectively exert the microbial activity of the indigenous algicidal bacteria to inhibit cyanobacterial bloom.
Disclosure of Invention
Aiming at the defects, the invention provides an indigenous algicidal bacterium activating agent which can be loaded on a self-prepared biomass carbon-nano iron/micro-filtration membrane polymer carrier and consists of polyvinyl alcohol, sodium alginate, calcium chloride and compound microbion, and the activating agent can achieve a slow release effect by adopting the self-prepared biomass carbon-nano iron/micro-filtration membrane polymer carrier, effectively retain the activity of the activating agent, and is further applied to eutrophication water bodies, thereby effectively preventing cyanobacterial bloom.
The invention provides the following technical scheme: indigenousThe algicidal bacteria activating medicament comprises polyvinyl alcohol (PVA), sodium Alginate (SA) and calcium chloride (CaCl) in a mass ratio of 550:35:12:3 2 ) The composite medicament is loaded on a biomass carbon-nano iron/microfiltration membrane polymer carrier, and the biomass carbon is formed by the nano iron/microfiltration membrane polymer in parts by weight and comprises the following components:
further, the microfiltration membrane polymer monomer comprises one or more of synthetic polyethersulfone, polymethyl methacrylate, polyurethane and polytetrafluoroethylene.
Further, the recyclable waste raw material is one or more of waste crop corn straw, waste crop wheat straw, waste furniture leftover materials and waste bamboo materials.
Further the ferrous salt is FeSO 4 、FeCl 2 、(CH 3 COO) 2 Fe or Fe (NO) 3 ) 2 One of them.
Further, the ferrous salt crystal water is FeSO 4 ·7H 2 O、FeCl 2 ·4H 2 O、 (CH 3 COO) 2 Fe·4H 2 O or Fe (NO) 3 ) 2 ·9H 2 One of O.
Further, the preparation method of the biomass charcoal in the nano-iron/micro-filtration membrane polymer carrier comprises the following steps:
1) Drying the recyclable waste raw materials in parts by weight at 50-60 ℃, crushing the materials by using a crusher, sieving the crushed materials to obtain pretreated particles, and placing the pretreated particles in a drying environment for later use;
2) Introducing nitrogen into a muffle furnace, under the condition of keeping an anaerobic environment, putting the pretreated particles obtained in the step 1) into a crucible, putting the crucible into the muffle furnace in the anaerobic environment, heating to 300-400 ℃ at a speed of 5 ℃/min, and then, carrying out heat preservation and sintering for 1-1.5 h to obtain biomass charcoal;
3) Placing 200ml-300ml ethanol water solution in nitrogen atmosphere for 20min-40min to remove dissolved oxygen, dissolving ferrous salt or ferrous salt crystal water and biomass charcoal obtained in the step 2) in the ethanol water solution, placing under nitrogen atmosphere, stirring at 200rpm-300rpm for 30min-45min, dropwise adding 5-10 parts NaBH during stirring 4 The solution is used for obtaining a biomass carbon-nano iron precursor precipitate, and the biomass carbon-nano iron precursor precipitate is alternately cleaned for 2-5 times by using deoxidized distilled water and deoxidized ethanol and dried under nitrogen to obtain biomass carbon-nano iron particles; the volume ratio of ethanol to water in the ethanol water solution is 2:3, and the NaBH is 4 NaBH in solution 4 The concentration of (2) is 10-15mmol/L;
4) Dissolving the microfiltration membrane polymer and the biomass carbon-nano iron particles obtained in the step 3) in 300-500ml of distilled water, heating in a water bath at 60-80 ℃ for 10-15 min, adding the potassium persulfate, stirring at 100-125 rpm for 10-15 min, continuously dropwise adding the N, N' -dimethylformamide in the stirring process, and standing for 15min after stirring;
5) And alternately cleaning the obtained precipitate with distilled water and ethanol for 2-3 times, and drying in a nitrogen atmosphere at a shady place to obtain the biomass carbon-nano iron/microfiltration membrane polymer carrier.
Further, the particle size of the pretreated particles obtained in the step 1) is 30-50 meshes.
Further, the NaBH 4 NaBH in solution 4 The concentration of (C) is 10-15mmol/L.
The invention also provides a method for preventing blue algae bloom by adopting the indigenous algicidal agent, which is used for activating the indigenous algicidal agent in the eutrophic water body, and 5kg-8kg of the indigenous algicidal agent is used per cubic meter so as to inhibit blue algae bloom in the eutrophic water body.
Further, the content of chlorophyll a in the eutrophic water body treated by the indigenous algicidal bacteria is less than 10ug/L, the transparency of the water body reaches more than 50cm, and the removal of the green color of the water body is remarkable.
The beneficial effects of the invention are as follows:
1. the invention discloses an activation method of indigenous algicidal bacteria based on the development of indigenous microorganism activation technology by a team, aiming at the problem of eutrophication of water body caused by exceeding of nitrogen and phosphorus, and the like.
2. The composite medicament consisting of the polyvinyl alcohol, the sodium alginate, the calcium chloride and the composite microorganism is loaded on the biomass carbon-nano iron/micro-filtration membrane polymer carrier prepared by self, and the activated medicament can achieve a slow release effect due to the adoption of the biomass carbon-nano iron/micro-filtration membrane polymer carrier prepared by self, so that the activity of the activated medicament is effectively reserved, and the activated medicament is further applied to eutrophic water bodies, and the cyanobacteria bloom phenomenon is effectively prevented.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
The embodiment provides an indigenous algicidal bacteria activating medicament, which comprises a composite medicament consisting of polyvinyl alcohol, sodium alginate, calcium chloride and composite microorganism in a mass fraction ratio of 550:35:12:3, wherein the composite medicament is loaded on a biomass carbon-nano iron/microfiltration membrane polymer carrier, and the biomass carbon is formed by the following components in parts by weight:
the recyclable waste raw material is corn stalks of waste crops.
The preparation method of the biomass charcoal in the nano-iron/micro-filtration membrane polymer carrier comprises the following steps:
1) Drying 50 parts of waste crop corn stalks at 50-60 ℃, crushing by using a crusher, sieving to obtain pretreated particles with the particle size of 30 meshes, and placing the pretreated particles in a drying environment for later use;
2) Introducing nitrogen into a muffle furnace, under the condition of keeping an anaerobic environment, putting the pretreated particles obtained in the step 1) into a crucible, putting the crucible into the muffle furnace in the anaerobic environment, heating to 300 ℃ at a speed of 5 ℃/min, and then, preserving heat and sintering for 1h to obtain biomass charcoal;
3) Placing 200 ml-ethanol water solution in nitrogen atmosphere for 20min to remove dissolved oxygen, and adding 20 parts FeSO 4 And dissolving the biomass charcoal obtained in the step 2) in the ethanol water solution, placing the biomass charcoal in a nitrogen atmosphere, stirring at 200rpm for 30min, and dropwise adding 5 parts of NaBH during stirring 4 The solution is used for obtaining a biomass carbon-nano iron precursor precipitate, and the biomass carbon-nano iron precursor precipitate is alternately cleaned for 2 times by using deoxidized distilled water and deoxidized ethanol and dried under nitrogen to obtain biomass carbon-nano iron particles; the volume ratio of ethanol to water in the ethanol water solution is 2:3, and the NaBH is 4 NaBH in solution 4 Is 10mmol/L;
4) Dissolving 30 parts of polyethersulfone and the biomass carbon-nano iron particles obtained in the step 3) in 300 distilled water, heating in a water bath at 60 ℃ for 10min, adding 8 parts of potassium persulfate, stirring at 100rpm for 10min, continuously dropwise adding 5 parts of N, N' -dimethylformamide in the stirring process, and standing for 15min after stirring;
5) And (3) alternately cleaning the obtained precipitate with distilled water and ethanol for 2 times, and drying in a nitrogen atmosphere at a shady place to obtain the biomass carbon-nano iron/microfiltration membrane polymer carrier.
The present embodiment also provides a method for preventing cyanobacterial bloom using the indigenous algicidal agent according to any one of claims 1 to 8, wherein the indigenous algicidal agent is used to activate indigenous algicidal bacteria in an eutrophic water body, and 5kg of the indigenous algicidal agent is used per cubic meter to inhibit cyanobacterial bloom in the eutrophic water body
The content of chlorophyll a in the eutrophic water body treated by the indigenous algicidal bacteria is less than 10ug/L, and the transparency of the water body reaches more than 50 cm.
Example 2
The embodiment provides an indigenous algicidal bacteria activating medicament which is characterized by comprising a composite medicament consisting of polyvinyl alcohol, sodium alginate, calcium chloride and composite microbion in a mass ratio of 550:35:12:3, wherein the composite medicament is loaded on a biomass carbon-nano iron/microfiltration membrane polymer carrier, and the biomass carbon is formed by the nano iron/microfiltration membrane polymer in parts by weight and comprises the following components:
the recyclable waste raw material is waste furniture leftover materials.
The preparation method of the biomass charcoal in the nano-iron/micro-filtration membrane polymer carrier comprises the following steps:
1) Drying 55 parts of waste furniture leftover materials at 55 ℃, crushing the waste furniture leftover materials by a crusher, sieving the crushed waste furniture leftover materials to obtain pretreated particles with the particle size of 40 meshes, and placing the pretreated particles in a drying environment for standby;
2) Introducing nitrogen into a muffle furnace, under the condition of keeping an anaerobic environment, putting the pretreated particles obtained in the step 1) into a crucible, putting the crucible into the muffle furnace in the anaerobic environment, heating to 350 ℃ at a speed of 5 ℃/min, and then, preserving heat and sintering for 1.25h to obtain biomass charcoal;
3) An aqueous ethanol solution having a volume of 250ml was placed under a nitrogen atmosphere for 30 minutes to remove dissolved oxygen therein, 25 parts of (CH 3 COO) 2 Fe·4H 2 O and the biomass charcoal obtained in the step 2) are dissolved inIn the ethanol water solution, placing under the condition of nitrogen atmosphere, stirring for 40min at 250rpm, and dropwise adding 8 parts of NaBH during stirring 4 The solution is used for obtaining a biomass carbon-nano iron precursor precipitate, and the biomass carbon-nano iron precursor precipitate is alternately cleaned for 4 times by using deoxidized distilled water and deoxidized ethanol and dried under nitrogen to obtain biomass carbon-nano iron particles; the volume ratio of ethanol to water in the ethanol water solution is 2:3, naBH 4 NaBH in solution 4 Is 12mmol/L;
4) Dissolving 32 parts of polytetrafluoroethylene and the biomass carbon-nano iron particles obtained in the step 3) in 400ml of distilled water, heating in a water bath at 70 ℃ for 12min, adding 11 parts of potassium persulfate, stirring at 110rpm for 12min, continuously dropwise adding 8 parts of N, N' -dimethylformamide in the stirring process, and standing for 15min after stirring;
5) And (3) alternately cleaning the obtained precipitate with distilled water and ethanol for 3 times, and drying in a nitrogen atmosphere at a shady place to obtain the biomass carbon-nano iron/microfiltration membrane polymer carrier.
The embodiment also provides a method for preventing cyanobacterial bloom by adopting the indigenous algicidal agent, wherein the indigenous algicidal agent is adopted to activate the indigenous algicidal agent in the eutrophic water body, and 6.5kg of the indigenous algicidal agent is used per cubic meter to inhibit cyanobacterial bloom in the eutrophic water body
The content of chlorophyll a in the eutrophic water body treated by the indigenous algicidal bacteria is less than 10ug/L, and the transparency of the water body reaches more than 50 cm.
Example 3
The embodiment provides an indigenous algicidal bacteria activating medicament, which comprises a composite medicament consisting of polyvinyl alcohol, sodium alginate, calcium chloride and composite microorganism in a mass fraction ratio of 550:35:12:3, wherein the composite medicament is loaded on a biomass carbon-nano iron/microfiltration membrane polymer carrier, and the biomass carbon is formed by the following components in parts by weight:
the recyclable waste raw material is waste bamboo material.
The preparation method of the biomass charcoal in the nano-iron/micro-filtration membrane polymer carrier comprises the following steps:
1) Drying 60 parts of waste bamboo materials at 560 ℃, crushing the waste bamboo materials by using a crusher, sieving the crushed waste bamboo materials to obtain pretreated particles with the particle size of 50 meshes, and placing the pretreated particles in a drying environment for later use;
2) Introducing nitrogen into a muffle furnace, under the condition of keeping an anaerobic environment, putting the pretreated particles obtained in the step 1) into a crucible, putting the crucible into the muffle furnace in the anaerobic environment, heating to 400 ℃ at a speed of 5 ℃/min, and then, preserving heat and sintering for 1.5 hours to obtain biomass charcoal;
3) Placing 300ml ethanol water solution in nitrogen atmosphere for 40min to remove dissolved oxygen, adding 30 parts FeCl 2 ·4H 2 O and the biomass charcoal obtained in the step 2) are dissolved in the ethanol water solution, and are placed under the condition of nitrogen atmosphere and stirred for 45min at the speed of 300rpm, and 10 parts of NaBH is added dropwise during the stirring process 4 The solution is used for obtaining a biomass carbon-nano iron precursor precipitate, and the biomass carbon-nano iron precursor precipitate is alternately cleaned for 5 times by using deoxidized distilled water and deoxidized ethanol and dried under nitrogen to obtain biomass carbon-nano iron particles; the volume ratio of ethanol to water in the ethanol water solution is 2:3, and the NaBH is 4 NaBH in solution 4 Is 15mmol/L;
4) Dissolving 35 parts of polyurethane and the biomass carbon-nano iron particles obtained in the step 3) in 500ml of distilled water, heating in a water bath at 80 ℃ for 15min, adding 15 parts of potassium persulfate, stirring at 125rpm for 15min, continuously dropwise adding 10 parts of N, N' -dimethylformamide in the stirring process, and standing for 15min after stirring;
5) And (3) alternately cleaning the obtained precipitate with distilled water and ethanol for 2 times, and drying in a nitrogen atmosphere at a shady place to obtain the biomass carbon-nano iron/microfiltration membrane polymer carrier.
The embodiment also provides a method for preventing cyanobacterial bloom by adopting the indigenous algicidal agent, wherein the indigenous algicidal agent is adopted to activate the indigenous algicidal agent in the eutrophic water body, and 8kg of the indigenous algicidal agent is used per cubic meter to inhibit cyanobacterial bloom in the eutrophic water body
The content of chlorophyll a in the eutrophic water body treated by the indigenous algicidal bacteria is less than 10ug/L, and the transparency of the water body reaches more than 50 cm.
While the invention has been described with reference to a preferred embodiment, various modifications may be made and equivalents may be substituted for elements thereof without departing from the scope of the invention. In particular, as long as there is no conflict in technical solutions, the technical features mentioned in the respective embodiments may be combined in any manner. The present invention is not limited to the specific embodiments disclosed herein, but encompasses all technical solutions falling within the scope of the claims.
Claims (9)
1. The indigenous algicidal bacteria activating medicament is characterized by comprising a composite medicament consisting of polyvinyl alcohol, sodium alginate, calcium chloride and composite microorganism in a mass fraction ratio of 550:35:12:3, wherein the composite medicament is loaded on a biomass charcoal-nano iron/micro-filtration membrane polymer carrier, and the biomass charcoal-nano iron/micro-filtration membrane polymer comprises the following components in parts by weight:
the preparation method of the biomass charcoal-nano iron/microfiltration membrane polymer carrier comprises the following steps:
1) Drying the recyclable waste raw materials in parts by weight at 50-60 ℃, crushing the materials by using a crusher, sieving the crushed materials to obtain pretreated particles, and placing the pretreated particles in a drying environment for later use;
2) Introducing nitrogen into a muffle furnace, under the condition of keeping an anaerobic environment, putting the pretreated particles obtained in the step 1) into a crucible, putting the crucible into the muffle furnace in the anaerobic environment, heating to 300-400 ℃ at a speed of 5 ℃/min, and then, carrying out heat preservation and sintering for 1-1.5 h to obtain biomass charcoal;
3) Placing 200ml-300ml ethanol water solution in nitrogen atmosphere for 20min-40min to remove dissolved oxygen, dissolving ferrous salt or ferrous salt crystal water and biomass charcoal obtained in the step 2) in the ethanol water solution, placing under nitrogen atmosphere, stirring at 200rpm-300rpm for 30min-45min, dropwise adding 5-10 parts NaBH during stirring 4 The solution is used for obtaining a biomass charcoal-nano iron precursor precipitate, deoxygenated distilled water and deoxygenated ethanol are used for alternately cleaning for 2-5 times, and the solution is dried under nitrogen to obtain biomass charcoal-nano iron particles; the volume ratio of ethanol to water in the ethanol water solution is 2:3;
4) Dissolving the microfiltration membrane polymer monomer and the biomass charcoal-nano iron particles obtained in the step 3) in 300-500ml of distilled water, heating in a water bath at 60-80 ℃ for 10-15 min, adding the potassium persulfate in the weight part, stirring at 100-125 rpm for 10-15 min, continuously dropwise adding the N, N' -dimethylformamide in the stirring process, and standing for 15min after stirring;
5) And alternately cleaning the obtained precipitate with distilled water and ethanol for 2-3 times, and drying in a nitrogen atmosphere at a shady place to obtain the biomass charcoal-nano iron/microfiltration membrane polymer carrier.
2. The indigenous algicidal agent of claim 1, wherein the microfiltration membrane polymer monomer comprises one or more of synthetic polyethersulfone, polymethyl methacrylate, polyurethane, and polytetrafluoroethylene.
3. The indigenous algicidal agent of claim 1, wherein the recyclable waste raw material is one or more of waste crop corn stover, waste crop wheat straw, waste furniture trim, and waste bamboo material.
4. The indigenous algicidal agent of claim 1, wherein the ferrous salt is FeSO 4 、FeCl 2 、(CH 3 COO) 2 Fe or Fe (NO) 3 ) 2 One of them.
5. The indigenous algicidal agent of claim 1, wherein the ferrous salt crystallization water is FeSO 4 ·7H 2 O、FeCl 2 ·4H 2 O、(CH 3 COO) 2 Fe·4H 2 O or Fe (NO) 3 ) 2 ·9H 2 One of O.
6. The indigenous algicidal agent of claim 1, wherein the pretreated particles obtained in step 1) have a particle size of 30 mesh to 50 mesh.
7. The indigenous algicidal agent of claim 1, wherein the NaBH 4 NaBH in solution 4 The concentration of (C) is 10-15mmol/L.
8. A method for preventing cyanobacterial bloom using the indigenous algicidal agent according to any one of claims 1-7, wherein the indigenous algicidal agent is used to activate indigenous algicidal bacteria in an eutrophic water body, using 5kg-8kg of the indigenous algicidal agent per cubic meter, to inhibit cyanobacterial bloom in the eutrophic water body.
9. The method of claim 8, wherein the eutrophic water body after being treated by indigenous algicidal bacteria has chlorophyll a content of less than 10ug/L and water body transparency of more than 50 cm.
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