CN108892243A - A kind of method of alga-bacterium symbiosis system purification pig factory waste water - Google Patents
A kind of method of alga-bacterium symbiosis system purification pig factory waste water Download PDFInfo
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- CN108892243A CN108892243A CN201810687904.4A CN201810687904A CN108892243A CN 108892243 A CN108892243 A CN 108892243A CN 201810687904 A CN201810687904 A CN 201810687904A CN 108892243 A CN108892243 A CN 108892243A
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- bacterium
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/14—NH3-N
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/16—Total nitrogen (tkN-N)
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Water Supply & Treatment (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Botany (AREA)
- Ecology (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a kind of methods of alga-bacterium symbiosis system purification pig factory waste water, and nearly tool thorn chain belt algae is separated specially from Pig raising wastewater, is stored in culture medium, is placed on shaking table and cultivates 24 hours, light intensity 5000-8000lux, 10-35 DEG C of temperature；The microalgae for taking culture to logarithmic growth phase is centrifuged 10min, using ultrapure water resuspension, in triplicate, is concentrated spare；Microalgae in step (2) is launched in pig factory waste water, Water warfare bacterium is added, and controlling frond density in the processing of all addition microalgaes is 0.1g/L, by frond：Thallus (according to the living bacteria count of each microbial inoculum) is than about 1：1 calculates the additive amount of Water warfare bacterium.The present invention reduces the discharges of pollutant, improve the resource utilization during pig-breeding.
Description
Technical field
The invention belongs to pig-breeding technical field more particularly to the methods of purification of waste water.
Background technique
Pig raising wastewater as " resource for misplacing position ", all the time all with Da mark emission reduction processing thought account for it is leading, and
The utilization to its resource characteristics is not fully considered, is resulted in waste of resources.Pig raising wastewater is after Anaerobic Digestion, still containing a large amount of
The nutriments such as nitrogen, phosphorus, the nutriment that they can be used as microalgae is used for the cultivation of microalgae, and most microalgaes has energy
The utilization of resources of source, feed and medicine etc. is worth, and the microalgae of harvest can be used for medical industry, animal feed and biological bavin
The raw material of oil etc., especially energy microalgae, are utilized with its low-risk and the relying on property such as high production rate become the current energy of solution
Crisis and a kind of very promising approach of environmental problem, received much attention in recent years.So being provided with environment with microalgae processing waste water
With resource double meaning.However, individually the effect is unsatisfactory for microalgae processing waste water, when additional mushroom, algae, bacterium phase interaction are utilized
With building alga-bacterium symbiosis system come when handling waste water, algae bio amount harvest is more, and the pollutants removal rates such as nitrogen, phosphorus are also more
It is high.
On the one hand, directly cultivate that microalgae is often feeding not to live using Pig raising wastewater, because the growth of microalgae may be by height
The inhibition of the factors such as concentration ammonia nitrogen, high organic loading or low dissolved oxygen or light transmittance, the photosynthesis of microalgae be affected without
Conducive to growth.Usually first by Pig raising wastewater through anaerobic digestion etc. before being used as microdisk electrode.Pretreatment is to eliminate or avoid its right
The inhibition of micro algae growth.On the other hand, the usually built anaerobic oxidation pond that is equipped in the pig farm of scale is handled as wastewater fermentation,
Technical maturity, first processing routine of the anaerobic digestion as Pig raising wastewater, can be generated by the fermentation of organic component can be again
Raw the energy (biogas) and organic matter can be restored, reduce Waste volume and stink.However because microorganism usually lacks enough autotrophys
Inorganic nitrogen metabolic mechanism, the nutriments such as ammonia nitrogen cannot substantially reduce in anaerobic fermentation, and can generate more bioavailable
Nitrogen form, such as ammonia nitrogen, total phosphorus, anaerobic digestion solution is still the higher sewage of pollutional load, and is handled with usual way
It is difficult to up to standard, it is necessary to find suitable method and carry out advanced treating.
In order to reduce the environmental pollution in pig-breeding, the regeneration of resource is sufficiently realized, the present invention provides one seeds algae-bacterium
The method of cogeneration system purification pig factory waste water.
Summary of the invention
For nitrogen and phosphorus pollutants technical problem in removal piggery wastewater anaerobic digestion effluent, the present invention provides a kind of optimum conditions
The method of lower alga-bacterium symbiosis system purification pig factory waste water.
The present invention is achieved by the following technical programs.
The present invention provides a kind of methods of alga-bacterium symbiosis system purification pig factory waste water, it is characterised in that：
(1) microalgae is separated from Pig raising wastewater, is stored in culture medium, is placed on shaking table and is cultivated 24 hours, light intensity 5000-
8000lux, 10-35 DEG C of temperature；
(2) microalgae for taking culture to logarithmic growth phase is centrifuged 10min, using ultrapure water resuspension, in triplicate, is concentrated standby
With；
(3) microalgae in step (2) is launched in pig factory waste water, Water warfare bacterium is added, and controls the place of all addition microalgaes
Frond density is 0.1g/L in reason, by frond：Thallus (according to the living bacteria count of each microbial inoculum) is than about 1：1 calculating Water warfare is thin
The additive amount of bacterium.
The Water warfare bacterium is selected from COD degradation bacterium, composite bacterial, nitrobacteria etc..
The preferred nitrobacteria of Water warfare bacterium.
The microalgae is chlorella or nearly tool thorn chain belt algae.
Contain a large amount of nitrogen phosphorus organic matters in pig manure after fermentation, the selected nitrobacteria of the present invention and such as close tool of microalgae pierce chain belt
Algae synergistic effect, by purified treatment, ammonia nitrogen, total nitrogen, total phosphorus are greatly reduced in water quality, and therefore, such as close tool of the microalgae pierces chain belt
Algae-nitrobacteria cogeneration system is suitable for the purified treatment of Wastewater from Pig Farm.The present invention reduces the discharges of pollutant, improve
Resource utilization during pig-breeding.
Specific embodiment
Technical solution of the present invention is described further below, but claimed range is not limited to.
Way of example：
Nearly tool thorn chain belt algae is separated from Pig raising wastewater, is stored in 500mL culture medium, is placed in 120rpm on shaking table and is trained
It supports, setting 24H illumination, light intensity 7000lux, 10-35 DEG C of temperature；The microalgae for taking culture to logarithmic growth phase is centrifuged in 8000rpm
10min in triplicate, is concentrated spare using ultrapure water resuspension.Waste water 500mL is taken to be placed in 500mL conical flask respectively, if
It sets and adds microalgae, COD degradation bacterium, composite bacterial, nitrobacteria, microalgae-COD degradation bacterium, microalgae-composite bacterial, micro- respectively
7 kinds of algae-nitrobacteria processing, and the space management of not additional strain and frond, every group setting two parallel.
It is found by detection, the cogeneration system that additional COD degradation bacterium, composite bacterial, nitrobacteria are constituted with microalgae respectively
And individually microalgae can be grown in simulation piggery wastewater anaerobic digestion effluent, corresponding microalgae maximum biomass is respectively
The growth microalgae of 1.92g/L (6d), 0.88g/L (2d), 4.64g/L (5d), 0.89g/L (2d), additional nitrobacteria pair promote
Effect is apparently higher than other two classes bacteriums.
PH is more when the system that microalgae and nitrobacteria co-culture is than other bacteriums and microalgae co-cultivation or independent microdisk electrode
Close to neutrality, it is conducive to micro algae growth.Ammonia nitrogen, total nitrogen, the equal highest of total tp removal rate in microalgae-nitrobacteria co-culture system, point
It Wei 51.2%, 45.7%, 51.2%.
COD removal effect is worst when microalgae-nitrobacteria co-cultures, and removal rate only has 55.1%, and microalgae is thin with other
COD removal rate is 80% or more when bacterium co-cultures and microalgae is individually cultivated.Although in conclusion nearly tool thorn chain belt algae-nitrification
COD removal effect is worst in bacterium co-culture system, but investigates in terms of micro algae growth situation and denitrogenation of waste water dephosphorization efficiency, should
System has outstanding advantage, therefore additional nitrobacteria is selected closely to have the growth for piercing chain belt algae and purification waste water ability to strengthen,
Construct alga-bacterium symbiosis system.
Claims (4)
1. a kind of method of alga-bacterium symbiosis system purification pig factory waste water, it is characterised in that：
(1) microalgae is separated from Pig raising wastewater, is stored in culture medium, is placed on shaking table and is cultivated 24 hours, light intensity 5000-
8000lux, 10-35 DEG C of temperature；
(2) microalgae for taking culture to logarithmic growth phase is centrifuged 10min, using ultrapure water resuspension, in triplicate, is concentrated spare；
(3) microalgae in step (2) is launched in pig factory waste water, Water warfare bacterium is added, in the processing for controlling all addition microalgaes
Frond density is 0.1g/L, by frond：Thallus (according to the living bacteria count of each microbial inoculum) is than about 1：1 calculates Water warfare bacterium
Additive amount.
2. a kind of method of alga-bacterium symbiosis system purification pig factory as described in claim 1 waste water, it is characterised in that：The water
Cleaning bacteria is selected from COD degradation bacterium, composite bacterial, nitrobacteria etc..
3. a kind of method of alga-bacterium symbiosis system purification pig factory as described in claim 1 waste water, it is characterised in that：The water
The preferred nitrobacteria of cleaning bacteria.
4. a kind of method of alga-bacterium symbiosis system purification pig factory as described in claim 1 waste water, it is characterised in that：It is described micro-
Algae is chlorella or nearly tool thorn chain belt algae.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810687904.4A CN108892243A (en) | 2018-06-28 | 2018-06-28 | A kind of method of alga-bacterium symbiosis system purification pig factory waste water |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109942091A (en) * | 2019-04-09 | 2019-06-28 | 哈尔滨学院 | Bacterium algae attaching biological fibre bed and preparation method thereof and its method of intensive treatment pig denitrogenation of waste water dephosphorization |
| CN114349174A (en) * | 2022-01-17 | 2022-04-15 | 大连理工大学 | Method for removing tetracycline based on algae-bacteria complex |
| CN114456942A (en) * | 2022-01-20 | 2022-05-10 | 广西大学 | Chain-belt algae for treating high-concentration molasses alcohol wastewater and application thereof |
-
2018
- 2018-06-28 CN CN201810687904.4A patent/CN108892243A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109942091A (en) * | 2019-04-09 | 2019-06-28 | 哈尔滨学院 | Bacterium algae attaching biological fibre bed and preparation method thereof and its method of intensive treatment pig denitrogenation of waste water dephosphorization |
| CN109942091B (en) * | 2019-04-09 | 2022-04-29 | 哈尔滨学院 | Bacterial-algae attached biological fiber bed, preparation method thereof and method for strengthening treatment of pig wastewater for nitrogen and phosphorus removal |
| CN114349174A (en) * | 2022-01-17 | 2022-04-15 | 大连理工大学 | Method for removing tetracycline based on algae-bacteria complex |
| CN114349174B (en) * | 2022-01-17 | 2022-10-04 | 大连理工大学 | Method for removing tetracycline based on algae-bacteria complex |
| CN114456942A (en) * | 2022-01-20 | 2022-05-10 | 广西大学 | Chain-belt algae for treating high-concentration molasses alcohol wastewater and application thereof |
| CN114456942B (en) * | 2022-01-20 | 2023-12-15 | 广西大学 | Chain belt algae for treating high-concentration molasses alcohol wastewater and application thereof |
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Application publication date: 20181127 |