CN102732425A - Method for producing microalgae through utilizing livestock and poultry excrement primary wastewater - Google Patents
Method for producing microalgae through utilizing livestock and poultry excrement primary wastewater Download PDFInfo
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Abstract
The invention discloses a method for producing microalgae through utilizing livestock and poultry excrement primary wastewater. The method comprises the following steps: 1, wastewater processing: flocculating the livestock and poultry excrement primary wastewater, taking the resulting supernatant, adding water to dilute to the COD being below 1000mg/L, letting ozone in to carry out aeration disinfection, and allowing the obtained mixture to stand; and 2, microalga culture: inoculating alga strains into the liquid obtained in step 1, carrying out illumination culture, letting air in during the culture process, and letting CO2 in to maintain the pH value of the liquid at 5-7. The preprocessing method of the livestock and poultry excrement primary wastewater allows the microalgae to be cultured through using the primary wastewater without wastewater anaerobic digestion, and complex florae in the wastewater to be removed through ozone disinfection, so the culture system stably runs, the culture cost of the microalgae is reduced, the biomass of the microalgae is improved, and it is in favor of the realization of the scale culture of the microalgae, and the resource utilization of the livestock and poultry excrement wastewater.
Description
Technical field
The present invention relates to a kind of method of utilizing the feces of livestock and poultry primary wastewater to produce little algae.
Background technology
The our times energy system is being faced with the rapid transformation to the energy supply system of low-carbon (LC), efficient, environmental protection.But there is lack of raw materials serious its mass-producing of restriction and industrialization, raw material has become the principal element of decision biofuel price.For ensureing that the development of Future in China biofuel has great significance, and uses little algae resource to become the research focus of present field of biodiesel oil as the oil sources object of biofuel.
Little algae is one type makes a living with freshwater, and growth and breeding is rapid, and living weight is big, can utilize photoautotrophy, sustainable supply biomass resource, and utilizing the waste water of livestock poultry cultivating microalgae is the fabulous bridge of realizing low-cost suitability for industrialized production of biomass and discharge of wastewater resource utilization.It is advantageous that: 1. little algae has high photosynthesis ability, can effectively utilize sun power fixation of C O
2, inorganics is converted into hydrogen, high unsaturated alkane, grease equal energy source material.2. the oleaginousness of little algae is higher, and the grease of production of units area production generally is higher than oil crops; 3. cultivating microalgae can effectively reduce cost in waste water of livestock poultry; The renewable energy resources technology of waste water of livestock poultry cultivating microalgae has environmental protection and economy, meets nature round-robin unique advantage.
Present stage little algae cultivation mainly in synthetic medium, be carbon source with the carbonic acid gas, illumination with mix under the stirring condition; Cost is higher; Little algae can be used for the deep purifying of secondary waste water traditionally, adopts through the livestock and poultry of anaerobic treatment and the secondary waste water of sanitary wastewater and carries out both culturing microalgae, and to overcome higher organic content, complicated flora and water turbidity are to the inhibition of micro algae growth.In this area, the feces of livestock and poultry primary wastewater is meant the waste water that obtains after the feces of livestock and poultry of its feeding field staticly settles, and secondary waste water is meant the waste water after primary wastewater is carried out anaerobic treatment.Because anaerobic digestion needs very big reaction volume and investment; More domestic middle-size and small-size its feeding fields do not have anaerobic processing device; Thereby need development effectively, simple and can utilize the method for pig farm primary wastewater cultivating microalgae; Realize that little algae utilizes abundant organic matter in the feces of livestock and poultry primary wastewater, be converted into living weight and accumulate greasy resource utilization.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of method of utilizing the feces of livestock and poultry primary wastewater to produce little algae, comprise the steps:
1) wastewater treatment: the feces of livestock and poultry primary wastewater is carried out flocculation treatment, gets supernatant, thin up to COD be below the 1000mg/L, feed ozonation aerated sterilization, leave standstill then;
2) little algae is cultivated: the algae kind is inoculated in the step 1) gained liquid, carries out illumination cultivation, and bubbling air in the culturing process, and feed CO
2To keep liquid pH value 5 ~ 7.
As optimal technical scheme; The above-mentioned feces of livestock and poultry primary wastewater of utilizing is produced the method for little algae; Feed ozonation aerated sterilization in the step 1), the ozone concn in the liquid is reached more than 2.8 mg/L, the ozone concn that leaves standstill then to the liquid is reduced to below the 0.1mg/L.
As optimal technical scheme, the above-mentioned feces of livestock and poultry primary wastewater of utilizing is produced the method for little algae, and step 1) is flocculation agent with the chitosan, is that coagulant aids carries out flocculation treatment to the feces of livestock and poultry primary wastewater with the sodium-alginate.Preferably, chitosan and sodium-alginate carry out flocculation treatment with the ratio adding feces of livestock and poultry primary wastewater of 5 ~ 10g/L respectively.
As optimal technical scheme, the above-mentioned feces of livestock and poultry primary wastewater of utilizing is produced the method for little algae, and step 1) is carried out flocculation treatment to the feces of livestock and poultry primary wastewater, gets supernatant, thin up to COD be 500 ~ 1000mg/L.
As optimal technical scheme, the above-mentioned feces of livestock and poultry primary wastewater of utilizing is produced the method for little algae, step 2) little algae that will be cultured to logarithmic phase is inoculated in the step 1) gained liquid with 5 ~ 20% inoculum size.
As optimal technical scheme, the above-mentioned feces of livestock and poultry primary wastewater of utilizing is produced the method for little algae, step 2) the little algae temperature of cultivating is 25 ℃ ~ 35 ℃.
As optimal technical scheme, the above-mentioned feces of livestock and poultry primary wastewater of utilizing is produced the method for little algae, step 2) intensity of illumination of illumination cultivation is 2000 lux ~ 4000 lux.
As optimal technical scheme, the above-mentioned feces of livestock and poultry primary wastewater of utilizing is produced the method for little algae, step 2) incubation time cultivated of little algae is 10 ~ 15 days.
As optimal technical scheme; The above-mentioned feces of livestock and poultry primary wastewater of utilizing is produced the method for little algae, step 2) the algae kind is inoculated in the step 1) gained liquid, carry out illumination cultivation; Successive bubbling air in the culturing process; Intermittently feed carbonic acid gas, the feeding of carbonic acid gas feeds the constant cultivation that carbonic acid gas is kept pH value 5 ~ 7 by the control of pH unit when pH raises.
The invention has the beneficial effects as follows:
The present invention utilizes the feces of livestock and poultry primary wastewater to produce the method for little algae, walks around the anaerobic digestion of elementary feces of livestock and poultry waste water.Because investment is huge too high with residence time water treatment; Make a lot of middle-size and small-size its feedings field not have the anaerobic treatment equipment of feces of livestock and poultry primary wastewater, and the pretreatment process of elementary feces of livestock and poultry waste water of the present invention, can be under the situation that does not possess wastewater anaerobic digestive; Use the primary wastewater cultivating microalgae; Ozone sterilization can be removed flora complicated in the waste water, makes cultivating system stable, reduces the cultivation cost of little algae; Improve the living weight of little algae, helped realizing the scale cultivation of little algae and the resource utilization of feces of livestock and poultry waste water.
Description of drawings
Fig. 1 is the liquid of pig manure primary wastewater flocculation in the specific embodiment of the invention, the different COD initial values in supernatant dilution back, behind ozone sterilization, the aeration, and COD change procedure figure in little algae culturing process.
Fig. 2 is the liquid of pig manure primary wastewater flocculation in the specific embodiment of the invention, the different COD initial values in supernatant dilution back, behind ozone sterilization, the aeration, and total nitrogen content change procedure figure in little algae culturing process.
Fig. 3 is the liquid of pig manure primary wastewater flocculation in the specific embodiment of the invention, the different COD initial values in supernatant dilution back, behind ozone sterilization, the aeration, and total phosphorous change procedure figure in little algae culturing process.
Fig. 4 is the liquid of pig manure primary wastewater flocculation in the specific embodiment of the invention, the different COD initial values in supernatant dilution back, behind ozone sterilization, the aeration, and ammonia-nitrogen content change procedure figure in little algae culturing process.
Fig. 5 concerns synoptic diagram between ozone concn and sterilization rate in the liquid in the embodiment.
Fig. 6 feeds gas with various, the figure of cell density of chlorella and fat content in the culturing process in the embodiment.
Embodiment
Below in conjunction with specific embodiment the present invention is described further so that those skilled in the art can better understand the present invention and implementing, but the embodiment that lifts not conduct to qualification of the present invention.
Below describe an embodiment (utilizing the pig manure primary wastewater) who utilizes the method that the livestock and poultry primary wastewater produces little algae provided by the invention in detail, specifically may further comprise the steps:
1) pretreatment mode of pig manure primary wastewater: in the pig manure primary wastewater, add flocculation agent chitosan and coagulant aids sodium-alginate with the ratio of 5-10g/L respectively, carry out flocculation treatment, the pH of flocculation is between 5.0 ~ 7.0.Not to the virulent composition of the growth of little algae, can make the algae normal growth in the supernatant that obtains after the flocculation.The elementary waste liquid of supernatant pig manure that obtains is diluted to initial COD value and is respectively 500 mg/L ~ 1000 mg/L.Feed ozone to its concentration and reach more than the 2.8mg/L, reach more than 97% through sterilization rate after the ozonize.Make the ozone concn in the liquid be reduced to (ozone concn is in the growth not influence of 0.1mg/L to little algae) below the 0.1mg/L after leaving standstill.
2) little algae is cultivated: the chlorella that will be cultured to logarithmic phase is inoculated into respectively in the step 1 gained liquid by 5 ~ 20% inoculum size (being preferably 10% inoculum size), carries out aerobic culture, and the temperature of cultivation is 25 ℃ ~ 35 ℃.In culturing process, feed the air (the feeding speed that is the nutrient solution air of 1L is 100 mL/min) of 100mL/minL with pneumatic pump always; The feeding of carbonic acid gas is controlled by the pH unit; PH can feed the constant cultivation that carbonic acid gas is kept pH value 5 ~ 7 when raising; To avoid the feeding growth that carbonic acid gas can cause the acidifying of nutrient solution and influence little algae always, avoid the waste of carbonic acid gas simultaneously.Intensity of illumination 2000lux ~ 4000lux is preferably 2000lux ~ 3000lux, and incubation time is 10 ~ 15 days.
In the specific implementation, the algae kind of present embodiment employing does
Chlorella pyrenoidosa, aeration after 24 hours the ozone concn in the liquid manure be 0.1mg/L, the growth of chlorella is not exerted an influence, can tolerate 1000mg COD/L pig manure primary wastewater, the raising of COD can cause the raising of nutrient solution turbidity and influence the normal growth of little algae.In the pig manure water without ozone sterilization, the growth of chlorella is unstable, usually causes the dead phenomenon of chlorella jaundice; Prove through experimental result repeatedly; Culture system is stable behind ozone sterilization, and incubation time is generally can gather in the crops frond in 10 ~ 13 days, and the water purification process also reaches balance.
In the specific implementation; With the feeding amount of pH control carbonic acid gas, compare with the method that in culturing process, feeds 5% carbonic acid gas and air Mixture commonly used always in the present embodiment, the living weight and the fat content of final frond are close; The decontamination effect improving of waste water is also similar; But effectively utilized carbonic acid gas, utilized the organic matter in the pig manure waste water simultaneously, the living weight of acquisition is 3 ~ 4 times that in synthetic medium, cultivate.
1) pretreatment mode of pig manure primary wastewater: in the pig manure primary wastewater, add the flocculation agent chitosan with the ratio of 10g/L respectively and do and the coagulant aids sodium-alginate, carry out flocculation treatment, the pH of flocculation is between 5.0 ~ 7.0.The elementary waste liquid of supernatant pig manure that obtains is diluted to initial COD value and is respectively 500mg/L, 700mg/L, 800mg/L, 1000mg/L.Feed ozone to its concentration and reach more than the 2.8mg/L, reach more than 97% through sterilization rate after the ozonize.Aeration makes the ozone concn in the liquid be reduced to 0.072mg/L.
2) little algae is cultivated: the chlorella that will be cultured to logarithmic phase is inoculated in the step 1 gained liquid by 10% inoculum size, carries out aerobic culture, and the temperature of cultivation is 25 ℃ ~ 35 ℃.In culturing process, feed the air of 100mL/minL with pneumatic pump always; The feeding of carbonic acid gas is controlled by the pH unit; PH can feed the constant cultivation that carbonic acid gas is kept pH value 5 ~ 7 when raising; To avoid the feeding growth that carbonic acid gas can cause the acidifying of nutrient solution and influence little algae always, avoid the waste of carbonic acid gas simultaneously.Intensity of illumination 2000lux ~ 4000lux, incubation time are 14 days.
Detect the change procedure of waste water each item index of different COD initial values in little algae culturing process.Shown in Fig. 1 ~ 4; Follow the cultivation of chlorella, COD value, total nitrogen content, total phosphorous and the ammonia-nitrogen content of waste water constantly reduce, and cultivate chlorella after 12 ~ 13 days; Initial COD value is the waste water of 500mg/L, 700mg/L, 800mg/L, 1000mg/L: the COD value is all reduced to about 300mg/L; Total nitrogen content is all reduced to below 20 mg/L, and total phosphorous is all reduced to below 5 mg/L, and ammonia-nitrogen content is all reduced to below the 5mg/L.Explain that utilizing method of the present invention to cultivate chlorella can carry out recycling treatment to the feces of livestock and poultry primary wastewater effectively.
The pretreatment mode of pig manure primary wastewater: in the pig manure primary wastewater, add the flocculation agent chitosan with the ratio of 5 g/L respectively and do and the coagulant aids sodium-alginate, carry out flocculation treatment, the pH of flocculation is between 5.0 ~ 7.0.The elementary waste liquid of supernatant pig manure that obtains is diluted to initial COD value and is 800mg/L.Feed ozone to its concentration and reach 2.58 mg/L, 2.86 mg/L, 3.1 mg/L respectively, detect sterilization rate.
As shown in Figure 5, feeding ozone to concentration is that sterilization rate is merely about 60% behind 2.58 mg/L, is that sterilization rate can reach more than 97% behind 2.86 mg/L or 3.1 mg/L and feed ozone to concentration.From economy principle, ozone sterilization of the present invention preferably feed to ozone concn more than 2.8mg/L.
Embodiment 3
1) pretreatment mode of pig manure primary wastewater: in the pig manure primary wastewater, add the flocculation agent chitosan with the ratio of 8 g/L respectively and do and the coagulant aids sodium-alginate, carry out flocculation treatment, the pH of flocculation is between 5.0 ~ 7.0.The elementary waste liquid of supernatant pig manure that obtains is diluted to initial COD value and is respectively 1000 mg/L.Feed ozone to its concentration and reach more than the 2.8mg/L, reach more than 97% through sterilization rate after the ozonize.Aeration makes the ozone concn in the liquid be reduced to below the 0.1mg/L.
2) little algae is cultivated: the chlorella that will be cultured to logarithmic phase is inoculated into respectively in the step 1 gained liquid by the inoculum size of 15 %, carries out aerobic culture, and the temperature of cultivation is 25 ℃ ~ 35 ℃.In culturing process always with feeding gas: a. respectively in the following manner only with the continuous bubbling air of pneumatic pump; B. feed carbonic acid gas and Air mixing gas continuously, the volume percent of carbonic acid gas is 5% to use the gas mixing device to make wherein; C. with the continuous bubbling air of pneumatic pump; The feeding of carbonic acid gas is controlled by the pH unit; Feed the constant cultivation that carbonic acid gas is kept pH value 5 ~ 7 when pH raises,, avoid the waste of carbonic acid gas simultaneously to avoid the feeding growth that carbonic acid gas can cause the acidifying of nutrient solution and influence little algae always.Intensity of illumination is 2000lux ~ 3000lux, and incubation time is 10 ~ 15 days.
The result is as shown in Figure 6, when with mode a bubbling air, and the very low about 1.1g/L of living weight, oil quantity is about 22%; And when feeding carbonic acid gas and Air mixing gas continuously with mode b, living weight is about 1.9 g/L, and oil quantity is about 30%; With the continuous bubbling air of mode c pneumatic pump, the feeding of carbonic acid gas feeds the constant cultivation that carbonic acid gas is kept pH value 5 ~ 7 by the control of pH unit when pH raises; Living weight is about 1.8 g/L; Oil quantity is about 30%, and it can reach the effect of mode b, has avoided the waste of carbonic acid gas simultaneously.
To sum up, core of the present invention is to make feces of livestock and poultry primary wastewater culturing microalgae to become possibility, reduces the cost that little algae is cultivated, and reduces each item sewage index of feces of livestock and poultry primary wastewater simultaneously, carries out the recycling treatment of waste water.
The present invention considers that many middle-size and small-size its feedings factory does not have the treatment facility of handling excrement and urine water; Provide simply and effectively to the feces of livestock and poultry primary wastewater carry out with pre-treatment after the method for culturing microalgae; Effectively reduce the COD of feces of livestock and poultry waste water, total nitrogen, total phosphorus and ammonia nitrogen; But obtain little algae of resource utilization simultaneously, help the development of treatment project of industrialization and the pig manure waste water of little algae.
The above embodiment is the preferred embodiment that proves absolutely that the present invention lifts, and protection scope of the present invention is not limited thereto.Being equal to that the technician in present technique field is done on basis of the present invention substitutes or conversion, all within protection scope of the present invention.Protection scope of the present invention is as the criterion with claims.
Claims (10)
1. a method of utilizing the feces of livestock and poultry primary wastewater to produce little algae is characterized in that, comprises the steps:
1) wastewater treatment: the feces of livestock and poultry primary wastewater is carried out flocculation treatment, gets supernatant, thin up to COD be below the 1000mg/L, feed ozonation aerated sterilization, leave standstill then;
2) little algae is cultivated: the algae kind is inoculated in the step 1) gained liquid, carries out illumination cultivation, and bubbling air in the culturing process, and feed CO
2To keep liquid pH value 5 ~ 7.
2. the method for utilizing the feces of livestock and poultry primary wastewater to produce little algae according to claim 1; It is characterized in that; Feed ozonation aerated sterilization in the step 1), the ozone concn in the liquid is reached more than 2.8 mg/L, the ozone concn that leaves standstill then to the liquid is reduced to below the 0.1mg/L.
3. the method for utilizing the feces of livestock and poultry primary wastewater to produce little algae according to claim 1 is characterized in that, step 1) is flocculation agent with the chitosan, is that coagulant aids carries out flocculation treatment to the feces of livestock and poultry primary wastewater with the sodium-alginate.
4. the method for utilizing the feces of livestock and poultry primary wastewater to produce little algae according to claim 3 is characterized in that, chitosan and sodium-alginate carry out flocculation treatment with the ratio adding feces of livestock and poultry primary wastewater of 5 ~ 10g/L respectively.
5. the method for utilizing the feces of livestock and poultry primary wastewater to produce little algae according to claim 1 is characterized in that step 1) is carried out flocculation treatment to the feces of livestock and poultry primary wastewater, gets supernatant, thin up to COD be 500 ~ 1000mg/L.
6. the method for utilizing the feces of livestock and poultry primary wastewater to produce little algae according to claim 1 is characterized in that step 2) little algae that will be cultured to logarithmic phase is inoculated in the step 1) gained liquid with 5 ~ 20% inoculum size.
7. the method for utilizing the feces of livestock and poultry primary wastewater to produce little algae according to claim 1 is characterized in that step 2) the little algae temperature of cultivating is 25 ℃ ~ 35 ℃.
8. the method for utilizing the feces of livestock and poultry primary wastewater to produce little algae according to claim 1 is characterized in that step 2) intensity of illumination of illumination cultivation is 2000 lux ~ 4000 lux.
9. the method for utilizing the feces of livestock and poultry primary wastewater to produce little algae according to claim 1 is characterized in that step 2) incubation time cultivated of little algae is 10 ~ 15 days.
10. the method for utilizing the feces of livestock and poultry primary wastewater to produce little algae according to claim 1; It is characterized in that step 2) the algae kind is inoculated in the step 1) gained liquid, carry out illumination cultivation; Successive bubbling air in the culturing process; Intermittently feed carbonic acid gas, the feeding of carbonic acid gas feeds the constant cultivation that carbonic acid gas is kept pH value 5 ~ 7 by the control of pH unit when pH raises.
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CN104152356A (en) * | 2014-07-30 | 2014-11-19 | 江苏理工学院 | Method for heterotrophic culture of microalgae by utilizing chicken manure degradation liquid |
CN107151055A (en) * | 2017-06-01 | 2017-09-12 | 中国农业大学 | A kind of method that waste water of livestock poultry environment increment is utilized |
CN107384832A (en) * | 2017-08-28 | 2017-11-24 | 中国科学院水生生物研究所 | A kind of method of inexpensive mass propgation Azotica |
CN110408544A (en) * | 2019-09-02 | 2019-11-05 | 浙江海洋大学 | Utilize the method for aquiculture waste water culture microalgae |
CN111944735A (en) * | 2020-09-03 | 2020-11-17 | 上饶师范学院 | Method for promoting growth of microalgae in livestock and poultry breeding wastewater |
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CN104152356B (en) * | 2014-07-30 | 2017-02-08 | 江苏理工学院 | Method for heterotrophic culture of microalgae by utilizing chicken manure degradation liquid |
CN106434355A (en) * | 2014-07-30 | 2017-02-22 | 江苏理工学院 | Method for heterotrophic culture of microalgae by using chicken manure degradation liquid |
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CN107384832A (en) * | 2017-08-28 | 2017-11-24 | 中国科学院水生生物研究所 | A kind of method of inexpensive mass propgation Azotica |
CN110408544A (en) * | 2019-09-02 | 2019-11-05 | 浙江海洋大学 | Utilize the method for aquiculture waste water culture microalgae |
CN111944735A (en) * | 2020-09-03 | 2020-11-17 | 上饶师范学院 | Method for promoting growth of microalgae in livestock and poultry breeding wastewater |
CN112028251A (en) * | 2020-09-03 | 2020-12-04 | 上饶师范学院 | Method for improving ammonia nitrogen removal effect of microalgae on livestock and poultry breeding wastewater |
CN111944735B (en) * | 2020-09-03 | 2023-07-04 | 上饶师范学院 | Method for promoting microalgae to grow in livestock and poultry breeding wastewater |
CN112159261A (en) * | 2020-10-10 | 2021-01-01 | 天津科技大学 | Method for producing liquid concentrated fertilizer from livestock and poultry manure |
CN112795486A (en) * | 2020-12-30 | 2021-05-14 | 兴源环境科技股份有限公司 | Method for synchronously producing ethanol by livestock and poultry manure microalgae culture |
CN112795486B (en) * | 2020-12-30 | 2023-09-19 | 兴源环境科技股份有限公司 | Method for synchronously producing ethanol by livestock and poultry manure microalgae cultivation |
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