CN106434355A - Method for performing heterotrophic cultivation on microalgae by chicken excrement degradation liquid - Google Patents

Method for performing heterotrophic cultivation on microalgae by chicken excrement degradation liquid Download PDF

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CN106434355A
CN106434355A CN201611136286.1A CN201611136286A CN106434355A CN 106434355 A CN106434355 A CN 106434355A CN 201611136286 A CN201611136286 A CN 201611136286A CN 106434355 A CN106434355 A CN 106434355A
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microalgae
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nitrogen
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chicken manure
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梁国斌
汪斌
刘维平
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Jiangsu University of Technology
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    • Y02E50/10—Biofuels, e.g. bio-diesel

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Abstract

The invention discloses a method for performing heterotrophic cultivation on microalgae by chicken excrement degradation liquid. The method comprises the following steps: degrading a chicken excrement diluent at a rotating speed of 40 rpm to 60 rpm to obtain low-nitrogen degradation liquid and degrading another chicken excrement diluent at a rotating speed of 240 rpm to 260 rpm to obtain high-nitrogen degradation liquid; and inoculating microalgae liquid which is cultivated on a shaker into a cultivation tank, performing heterotrophic cultivation on the microalgae for 180 to 220 hours to finish heterotrophic cultivation of the microalgae, wherein in the heterotrophic cultivation process, the high-nitrogen degradation liquid is added into a cultivation system at the earlier stage and the low-nitrogen degradation liquid is added in the cultivation system at the later stage. In the whole microalgae cultivation process, the chicken excrement high-nitrogen degradation liquid and the chicken excrement low-nitrogen degradation liquid are directly added into the cultivation liquid and an additional nitrogen source is not needed, so the whole process is high in efficiency, energy sources are saved and the cost is lowered.

Description

A kind of method of utilization chicken manure degradation solution Heterotrophic culture microalgae
The application is Application No. 201410366807.7, and the applying date is on July 30th, 2014, and invention and created name is The divisional application of the application for a patent for invention of " using the method for chicken manure degradation solution Heterotrophic culture microalgae ".
Technical field
The present invention relates to a kind of cultural method of microalgae is and in particular to a kind of utilization chicken manure degradation solution Heterotrophic culture microalgae Method.
Background technology
World's crude output persistently reduces it is contemplated that will settle to 7,500,000,000 barrels/year by 2018, just corresponds to nineteen fifty crude oil Yield.Biodiesel refers to, by oils and fatss and short chain alcohol in vegetable oil, Animal fat and extracellular microbial, obtain through ester exchange reaction Organic aliphatic acid Ester, as renewable alternative energy source, environment friendly and recyclability make it have very strong competing Strive power.Microalgae has the features such as growth cycle is short, speed is fast, thus by microdisk electrode synthesising biological oils and fatss and then prepares biological Diesel oil is one of most effective approach.
Microalgae can carry out photosynthetic culture using the luminous energy in nature and inorganic carbon source, but photosynthetic culture has life Slow, the final Biomass of long speed is low, the shortcoming of growth cycle length.In addition, microalgae can utilize under conditions of not needing illumination Organic substance carries out Heterotrophic culture as carbon source and nitrogen source, and Heterotrophic culture has the advantages that fast growth, final Biomass are high, energy Enough shorten the cycle.
The final purpose that microalgae biodiesel produces is to obtain maximum lipid production with minimum cost.With photosynthetic culture Compare, Heterotrophic culture microalgae primary concern is that the cost of carbon and nitrogen source.
Such as Chinese patent literature CN 102746992 A（Application number 201210244511.9）Disclose one kind using dirty The method of mud hydrolyzed solution Heterotrophic Mass Cultures of Chlorella, first takes the excess sludge of sewage treatment plants, supersound process after concentration；To surpass Mud after sonication is 180r/min in rotating speed, under conditions of temperature is 35 DEG C, carries out anaerobic hydrolysis 2 to 3 days；To obtain Add trace element and other nutrients in sludge hydrolytic liquid, after high pressure steam sterilization, obtain culture fluid；Culture fluid is cooled to After room temperature, chlorella is seeded in culture fluid, is placed in culture 150h～180h in shaken cultivation case, completes the training of chlorella Support.But the nitrogen content of sludge degradation liquid is not high, and in degradation solution, contain plurality of heavy metal, suppress micro algae growth.
Poultry manure (PM) is rich in nutritive salt, particularly nitrogen, and its capacity can reach 55.7g/L by combination.Additionally, except Outside abundant nitrogen, potassium, phosphorus, trace element such as magnesium, calcium, ferrum, copper, zinc etc. is also stored in PM degradation solution.Therefore PM is that one kind has valency The source of the main nutrients of value, if nutritive salt can be by efficient absorption, then for microdisk electrode, PM is definitely one Developable efficient resource.
Chicken manure (CM) as a kind of typical PM, rich in elements such as organic nitrogen, phosphorus.Chinese patent literature CN 103756908 （Application number 201410014303.9）Disclose a kind of compound method of organic microalgae culture solution, the chicken that will transport from chicken farm Excrement, is placed on playground, tans by the sun 8 days under conditions of temperature is 20 DEG C～28 DEG C, then removed with sieve stone in chicken manure, Husky, feather；Mix tanning by the sun dried chicken manure according to 1: 10 ratio with 100 DEG C of waste water, and be sufficiently stirred for；After stirring Solution soaking 3h obtaining, is sufficiently stirred for it in immersion process；The culture fluid obtaining after immersion passes through the screen cloth of 24 mesh Filter, the solute above filter screen is used in mixed way with the chicken manure that other are soaked, the solution obtaining is the former of bead algae culturing liquid Liquid；The culture fluid being filtrated to get stock solution is diluted, obtains bead algae culturing liquid；By 10%～20% chlorella algae kind with 80%～90% bead algae culturing liquid mixing, carries out chlorella culture.The nutrients such as the carbon in chicken manure, nitrogen are all with the shape of Organic substance Formula exists, and organic nitrogen can not be decomposed into inorganic nitrogen such as ammoniacal nitrogen and nitrate nitrogen thus by microalgae by the above-mentioned processing method to chicken manure Absorbed, thus the utilization of nutrients of chicken manure is relatively low.
Content of the invention
The technical problem to be solved is to provide one kind using chicken manure degradation solution Heterotrophic culture microalgae thus promoting The method of microalgae synthesising biological oils and fatss.
The technical scheme realizing the object of the invention is a kind of method of utilization chicken manure degradation solution Heterotrophic culture microalgae, including with Lower step：
1. chicken manure degraded；
Take the fresh chicken manure in chicken farm, with distilled water diluting, stir evenly and obtain chicken manure diluent.
Take chicken manure diluent to be transferred in triangular flask, seal bottleneck in the bottleneck gauze of triangular flask, then by triangular flask It is transferred to respectively in shaking table, degraded under the rotating speed of 40rpm～60rpm obtains the material after degraded after 4～6 days, be filtrated to get Low nitrogen degradation liquid, is transferred to Refrigerator store after low nitrogen degradation liquid sterilization stand-by.
Separately take chicken manure diluent to be transferred in triangular flask, seal bottleneck in the bottleneck gauze of triangular flask, then by triangle Bottle is transferred in shaking table respectively, and degraded under the rotating speed of 240rpm～260rpm obtains the material after degraded after 4～6 days, filter Obtain high nitrogen degradation liquid, be transferred to Refrigerator store after high nitrogen degradation liquid sterilization stand-by.
2. microalgae Heterotrophic culture；
First shaking table culture is carried out to microalgae seed, triangular flask loads micro algae culturing liquid, take microalgae cell from protecting storage inclined-plane It is inoculated in the culture fluid of the corresponding microalgae in triangular flask, then triangular flask is placed on shaking table, 140～160 turns of rotating speed of setting/ Minute, 28 DEG C～32 DEG C of cultivation temperature, cultivate 65～75 h.
Then the microalgae liquid obtaining after cultivating on shaking table is inoculated in culture tank with 8%～12% (v/v) inoculum concentration, culture Add the culture fluid of corresponding microalgae in tank, after microalgae Heterotrophic culture 180～220h, complete the Heterotrophic culture of microalgae；Wherein heterotrophism In incubation, start in the 100th～120 hour in Heterotrophic culture, add step with 14～16 mL/L/Day to cultivating system The rapid high nitrogen degradation liquid 1. preparing, then from the 100th～120 hour until culture period terminates, with 14～16 mL/L/Day to training Foster system adds the low nitrogen degradation liquid that 1. step prepares.
The above-mentioned steps total nitrogen of low nitrogen degradation liquid 1., nitrate nitrogen, ammonia-nitrogen content be respectively 0.50～0.8g/L, 130～ 260mg/L, 300～360mg/L；The total nitrogen of high nitrogen degradation liquid, nitrate nitrogen, ammonia-nitrogen content be respectively 1.05～1.5g/L, 780～ 910mg/L, 70～120mg/L.
Above-mentioned steps 2. microalgae Heterotrophic culture when, the pH of cultivating system controls 5.0～7.0,28～32 DEG C of temperature, ventilation Speed 0.8～1.2vvm, speed of agitator 180～220rpm.
As preferred, the pH adjusting agent of cultivating system is sulphuric acid or NaOH aqueous solution.
As preferred, the low nitrogen degradation liquid that 1. step obtains sterilizes 15～25 minutes at 115 DEG C～121 DEG C, transfer Preserve stand-by to 3 DEG C～6 DEG C of refrigerator；The high nitrogen degradation liquid that 1. step obtains sterilizes 15～25 points at 115 DEG C～121 DEG C Clock, be transferred to preserve at 3 DEG C～6 DEG C of refrigerator stand-by.
The present invention has positive effect：（1）The present invention when using chicken manure degradation solution Heterotrophic culture microalgae, first not With under the conditions of chicken manure is degraded, obtain the high degradation solution of nitrogen content and the low degradation solution of nitrogen content；Then by microalgae seed After being inoculated in its corresponding culture fluid, during i.e. front 100～120h is to cultivating system in culture period leading portion, add high nitrogen degradation liquid, This stage microalgae cell rapid development；Add low nitrogen degradation liquid in the back segment of culture period is to cultivating system, culture body now System is beneficial to microalgae grease synthesis；Culture terminates that rear microalgae frond amount is higher, and it is high to cultivate the fat content of the microalgae obtaining.
（2）Due in addition to abundant nitrogen, potassium, phosphorus, also contain in the chicken manure degradation solution of the present invention trace element such as magnesium, Calcium, ferrum, copper, zinc etc., during whole microdisk electrode, are directly added into chicken manure high and low nitrogen degradation liquid, no in culture fluid Nitrogen source need to be it is possible to additionally incorporate, thus whole process efficiency high, save energy and cost.
（3）The method process is simple of present invention culture microalgae, economy, environmental protection, meet the needs of recycling economy development, have Good prospects for commercial application.
Specific embodiment
（Embodiment 1）
The microalgae of the present embodiment Heterotrophic culture be chlorella (Chlorella protothecoides), buy from the Chinese Academy of Sciences American Type Culture Collection committee algae kind storehouse, deposit number is FACHB-3.
The method using chicken manure degradation solution Heterotrophic culture microalgae of the present embodiment comprises the following steps：
1. chicken manure degraded.
The first-selected condition to chicken manure degraded is screened.
Take fresh chicken manure 300mL in chicken farm, the moisture content of this chicken manure is 70%～75%, and being added thereto to same volume is The distilled water diluting of 300mL, the chicken manure diluent after stirring evenly is respectively charged in 6 triangular flasks, and filled in each triangular flask is dilute Chicken manure volume after releasing is 30mL～50mL, and in the present embodiment, the volume of every bottle of filled material is 30mL.
Seal bottleneck in the bottleneck gauze of each triangular flask, then each triangular flask is transferred in shaking table respectively, point Do not degrade 4～6 days under the rotating speed of 50rpm, 100rpm, 150rpm, 200rpm, 250rpm and 300rpm（In the present embodiment it is 5 days）Obtain the material after 6 parts of degradeds afterwards, be filtrated to get 6 parts of degradation solutions, every part of degradation solution sterilizes 20 minutes at 121 DEG C, turn Move to and preserve at 4 DEG C of refrigerator.
Measure ammonia nitrogen (NH in above-mentioned 6 parts of degradation solutions respectively4- N), nitrate nitrogen (NO3- N) and total nitrogen (TN) content, select wherein The degradation solution that total nitrogen content highest degradation solution and the minimum degradation solution of total nitrogen content use as the present embodiment microdisk electrode.
Detection finds that the total nitrogen content of the degradation solution for obtaining under conditions of 50rpm for the shaking speed is minimum, its total nitrogen, nitre Nitrogen, ammonia-nitrogen content be respectively 0.56～0.64g/L, 140～180mg/L, 330～360mg/L, using shaking speed 50rpm as The degradation condition of low nitrogen chicken manure degradation solution.
The total nitrogen content highest of the degradation solution for obtaining under conditions of 250rpm for the shaking speed, its total nitrogen, nitrate nitrogen, ammonia nitrogen contain Amount is respectively 1.18～1.34g/L, 830～860mg/L, 110～120mg/L, using shaking speed 250rpm as high nitrogen chicken manure The degradation condition of degradation solution.
After determining above-mentioned degradation condition, during chicken manure degraded：
Take fresh chicken manure 600mL in chicken farm, be added thereto to the distilled water diluting that same volume is 600mL, fill respectively after stirring evenly Enter in 2 triangular flasks, the chicken manure volume after the dilution being filled in each triangular flask is 100mL；Use in the bottleneck of each triangular flask Gauze seals bottleneck, then each triangular flask is transferred in shaking table respectively, degrades respectively under the rotating speed of 50rpm and 250rpm Obtain the material after 2 parts of degradeds after 5 days, be filtrated to get 2 parts of degradation solutions, every part of degradation solution sterilizes 20 minutes at 121 DEG C, transfer Preserve stand-by to 4 DEG C of refrigerator.
Degrade under the rotating speed of 50rpm acquisition degradation solution be low nitrogen degradation liquid, under the rotating speed of 250rpm degraded obtain Degradation solution be high nitrogen degradation liquid.
2. microalgae Heterotrophic culture.
First shaking table culture is carried out to chlorella seed：Prepare the triangular flask of 5 to 10 250mL, in the triangle of each 250mL Load 50mL bead algae culturing liquid in bottle, from protecting, the training taking a certain amount of chlorella cells to be inoculated in each triangular flask is store inclined-plane In nutrient solution, then each triangular flask is placed on shaking table, rotating speed 140～160rpm, 28 DEG C～32 DEG C of cultivation temperature, culture are set 65h～75 h.
Bold ' s basal media is removed in described bead algae culturing liquid（BBM）Outside culture medium, also include following material (g/L):Glucose 10.0, yeast powder 2.0, glycine 1.0.
Main matter in BBM culture medium is (g/L)： NaNO30.25, MgSO4.7H2O 0.075, NaCl 0.025, K2HPO4.3H2O 0.075, KH2PO40.175, CaCl2.2H2O 0.025, trace substance is (10-3g/L): ZnSO4.7H2O 8.82, MnCl2.4H2O 1.44, MoO30.71, CuSO4.5H2O1.57, Co (NO3)2.6H2O 0.49, H3BO311.42, EDTA 50.0, KOH 31.0, FeSO4.7H2O 4.98.
Then the bead algae solution obtaining after cultivating on shaking table is inoculated into the culture tank of 5L with 8%～12% (v/v) inoculum concentration In, in culture tank, bead algae culturing liquid volume is 2.5～3.5L, and during microalgae Heterotrophic culture, the pH of cultivating system controls 5.0～7.0,28～32 DEG C of temperature, Ventilation Rate 0.8～1.2vvm, speed of agitator 180～220rpm, incubation time 180～ 220h（The present embodiment is 200h）.
During Heterotrophic culture, start in the 100th～120 hour in Heterotrophic culture, with 14～16 mL/L/Day to training Foster system adds high nitrogen degradation liquid, adds according to the amount that 1L liquid volume every in culture tank adds 14～16 mL degradation solutions daily Plus；Then from the 100th～120 hour until culture period terminates, add low nitrogen degradation with 14～16 mL/L/Day to cultivating system Liquid.
The pH adjusting agent of cultivating system is 4M～6M（It is 5M in the present embodiment）Sulphuric acid or NaOH aqueous solution.
The frond amount of culture detection of end chlorella is 7.47～7.68g/L, and grease concentration is 4.56～4.73 g/L.
If not adding chicken manure degradation solution to cultivating system, only carry out Heterotrophic culture using bead algae culturing liquid, according to this reality Apply step condition of culture 2., the frond amount that culture terminates the chlorella of acquisition is 5.73～6.12g/L, grease concentration is 2.83～3.09 g/L.
It can be seen that the present embodiment passes through to add chicken manure degradation solution to cultivating system, and add different in different cultivation stages Rear microalgae frond amount is higher so that culture terminates for the degradation solution of nitrogen content, and it is high to cultivate the fat content of the microalgae obtaining.
（Embodiment 2）
Remaining is same as Example 1 for the method for the utilization chicken manure degradation solution Heterotrophic culture microalgae of the present embodiment, and difference is：
Step 2. in the microalgae cultivated be Fructus Gleditsia acupuncture algae（Chaetoceros gracilis）.During culture, culture fluid used is angle Thorn algae culturing liquid.Heterotrophic culture time 220h.
Culture terminate after detection Fructus Gleditsia acupuncture algae (Chaetoceros gracilis) frond amount be 3.34～3.67g/L, oil Lipid concentration is 1.13～1.25 g/L.
If not adding chicken manure degradation solution to cultivating system, according to the present embodiment step condition of culture 2., cultivating end and obtaining The frond amount of the Fructus Gleditsia acupuncture algae obtaining is 2.34～2.56g/L, and grease concentration is 0.61～0.72 g/L.
（Embodiment 3）
Remaining is same as Example 1 for the method for the utilization chicken manure degradation solution Heterotrophic culture microalgae of the present embodiment, and difference is：
Step 2. in the microalgae cultivated be microalgae qin formula rhombus algae（Psammodictyon panduriforme）.Institute during culture Culture fluid is microalgae qin formula rhombus algae culturing liquid.Heterotrophic culture time 190h.
Culture detects after terminating that the frond amount of microalgae qin formula rhombus algae is 0.82 g/L～0.96g/L, and grease concentration is 0.46～0.52 g/L.
If not adding chicken manure degradation solution to cultivating system, according to the present embodiment step condition of culture 2., cultivating end and obtaining The frond amount of the microalgae qin formula rhombus algae obtaining is 0.55～0.62g/L, and grease concentration is 0.23～0.28g/L.
（Embodiment 4）
Remaining is same as Example 1 for the method for the utilization chicken manure degradation solution Heterotrophic culture microalgae of the present embodiment, and difference is：
Step 1. in by 6 triangular flasks respectively in the rotating speed of 40rpm, 90rpm, 140rpm, 190rpm, 240rpm and 290rpm Lower degraded 4～6 days（It is 5 days in the present embodiment）Obtain the material after 6 parts of degradeds afterwards, be filtrated to get 6 parts of degradation solutions.Measure respectively Ammonia nitrogen (NH in above-mentioned 6 parts of degradation solutions4- N), nitrate nitrogen (NO3- N) and total nitrogen (TN) content, the degradation solution obtaining under conditions of 40rpm Total nitrogen content minimum, using shaking speed 40rpm as low nitrogen chicken manure degradation solution degradation condition；Obtain under conditions of 240rpm Degradation solution total nitrogen content highest, using shaking speed 240rpm as high nitrogen chicken manure degradation solution degradation condition.
Then under the rotating speed of 40rpm and 240rpm, degraded obtained 2 parts of degradation solutions, under the rotating speed of 40rpm after 6 days respectively Degraded obtain degradation solution be low nitrogen degradation liquid, its total nitrogen, nitrate nitrogen, ammonia-nitrogen content be respectively 0.51～0.57g/L, 132～ 148mg/L, 305～313mg/L.
The degradation solution of acquisition of degrading under the rotating speed of 240rpm is high nitrogen degradation liquid, and its total nitrogen, nitrate nitrogen, ammonia-nitrogen content are respectively For 1.09～1.21g/L, 780～820mg/L, 90～100mg/L.
Step 2. cultivate after terminating detect chlorella frond amount be 7.32～7.53g/L, grease concentration be 4.28～ 4.49 g/L.
If not adding chicken manure degradation solution to cultivating system, according to the present embodiment step condition of culture 2., cultivating end and obtaining The frond amount of the chlorella obtaining is 5.73～6.12g/L, and grease concentration is 2.83～3.09 g/L.
（Embodiment 5）
Remaining is same as Example 1 for the method for the utilization chicken manure degradation solution Heterotrophic culture microalgae of the present embodiment, and difference is：
Step 1. in by 6 triangular flasks respectively in the rotating speed of 60rpm, 110rpm, 160rpm, 210rpm, 260rpm and 310rpm Lower degraded 4～6 days（It is 5 days in the present embodiment）Obtain the material after 6 parts of degradeds afterwards, be filtrated to get 6 parts of degradation solutions.Measure respectively Ammonia nitrogen (NH in above-mentioned 6 parts of degradation solutions4- N), nitrate nitrogen (NO3- N) and total nitrogen (TN) content, the degradation solution obtaining under conditions of 60rpm Total nitrogen content minimum, using shaking speed 60rpm as low nitrogen chicken manure degradation solution degradation condition；Obtain under conditions of 260rpm Degradation solution total nitrogen content highest, using shaking speed 260rpm as high nitrogen chicken manure degradation solution degradation condition.
Then under the rotating speed of 60rpm and 260rpm, degraded obtained 2 parts of degradation solutions, under the rotating speed of 60rpm after 6 days respectively Degraded obtain degradation solution be low nitrogen degradation liquid, its total nitrogen, nitrate nitrogen, ammonia-nitrogen content be respectively 0.65～0.76g/L, 240～ 260mg/L, 320～350mg/L.
The degradation solution of acquisition of degrading under the rotating speed of 260rpm is high nitrogen degradation liquid, and its total nitrogen, nitrate nitrogen, ammonia-nitrogen content are respectively For 1.21～1.49g/L, 870～910mg/L, 70～90mg/L.
Step 2. cultivate after terminating detect chlorella frond amount be 7.51～7.72g/L, grease concentration be 4.49～ 4.68 g/L.
If not adding chicken manure degradation solution to cultivating system, according to the present embodiment step condition of culture 2., cultivating end and obtaining The frond amount of the chlorella obtaining is 5.73～6.12g/L, and grease concentration is 2.83～3.09 g/L.

Claims (2)

1. a kind of method using chicken manure degradation solution Heterotrophic culture microalgae is it is characterised in that comprise the following steps：
1. chicken manure degraded；
Take the fresh chicken manure in chicken farm, with distilled water diluting, stir evenly and obtain chicken manure diluent；
Take chicken manure diluent to be transferred in triangular flask, seal bottleneck in the bottleneck gauze of triangular flask, then triangular flask is shifted To shaking table, degraded under 40 revs/min～60 revs/min of rotating speed obtains the material after degraded after 4～6 days, be filtrated to get Low nitrogen degradation liquid, the total nitrogen of low nitrogen degradation liquid, nitrate nitrogen, ammonia-nitrogen content be respectively 0.50～0.8g/L, 130～260mg/L, 300 ～360mg/L, is transferred to Refrigerator store after low nitrogen degradation liquid sterilization stand-by；
Separately take chicken manure diluent to be transferred in triangular flask, seal bottleneck in the bottleneck gauze of triangular flask, then triangular flask is turned Move in shaking table, degraded under 240 revs/min～260 revs/min of rotating speed obtains the material after degraded after 4～6 days, filter Obtain high nitrogen degradation liquid, the total nitrogen of high nitrogen degradation liquid, nitrate nitrogen, ammonia-nitrogen content be respectively 1.05～1.5g/L, 780～910mg/L, 70～120mg/L, is transferred to Refrigerator store after high nitrogen degradation liquid sterilization stand-by；
2. microalgae Heterotrophic culture；
First shaking table culture is carried out to microalgae seed, triangular flask loads micro algae culturing liquid, take microalgae cell from protecting storage inclined-plane It is inoculated in the culture fluid of the corresponding microalgae in triangular flask, then triangular flask is placed on shaking table, 140～160 turns of rotating speed of setting/ Minute, 28 DEG C～32 DEG C of cultivation temperature, cultivate 65～75 h；
Then the microalgae liquid obtaining after cultivating on shaking table is inoculated in culture tank with 8%～12% (v/v) inoculum concentration, in culture tank Add the culture fluid of corresponding microalgae, after microalgae Heterotrophic culture 180～220h, complete the Heterotrophic culture of microalgae；
During microalgae Heterotrophic culture, the pH of cultivating system controls 5.0～7.0,28～32 DEG C of temperature, and Ventilation Rate 0.8～ 1.2vvm, 180～220 revs/min of speed of agitator, the pH value of cultivating system is adjusted with the sulphuric acid of 4M～6M or NaOH aqueous solution；
Described microalgae is chlorella, and bead algae culturing liquid includes Bold ' s basal media culture medium, glucose, yeast powder And glycine, each constituent content glucose 10.0 g/L, yeast powder 2.0 g/L, glycine 1.0 g/L；
Wherein during Heterotrophic culture, start in the 100th～120 hour in Heterotrophic culture, with 14～16 mL/L/Day to training Foster system adds the high nitrogen degradation liquid that 1. step prepares, then from the 100th～120 hour until culture period terminates, with 14～16 ML/L/Day adds, to cultivating system, the low nitrogen degradation liquid that 1. step prepares.
2. utilization chicken manure degradation solution Heterotrophic culture microalgae according to claim 1 method it is characterised in that：1. step obtains The low nitrogen degradation liquid arriving sterilizes 15～25 minutes at 115 DEG C～121 DEG C, be transferred to preserve at 3 DEG C～6 DEG C of refrigerator stand-by；Step The rapid high nitrogen degradation liquid 1. obtaining sterilizes 15～25 minutes at 115 DEG C～121 DEG C, is transferred to and preserves at 3 DEG C～6 DEG C of refrigerator Stand-by.
CN201611136286.1A 2014-07-30 2014-07-30 Method for performing heterotrophic cultivation on microalgae by chicken excrement degradation liquid Pending CN106434355A (en)

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CN201611136286.1A CN106434355A (en) 2014-07-30 2014-07-30 Method for performing heterotrophic cultivation on microalgae by chicken excrement degradation liquid
CN201410366807.7A CN104152356B (en) 2014-07-30 2014-07-30 Method for heterotrophic culture of microalgae by virtue of chicken manure degrading liquid

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