CN110066736A - The method and system of Cyclic culture microalgae - Google Patents
The method and system of Cyclic culture microalgae Download PDFInfo
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Abstract
The present invention relates to field of microalgae cultivation, disclose the method and system of Cyclic culture microalgae.The method of Cyclic culture microalgae includes: 1) to cultivate microalgae, obtains micro algae culturing liquid；2) micro algae culturing liquid of harvest is recycled in the circuit for being equipped with membrane filter system, the partially liq in micro algae culturing liquid penetrates the filter membrane of membrane filter system, to respectively obtain the algae solution of permeate and concentration in filter membrane two sides；Liquid reuse be will transmit through to step 1), molecular cut off≤30kDa of filter membrane.The invention also discloses the systems of Cyclic culture microalgae, the microalgae recovery unit of microalgae including microdisk electrode unit and for collecting the generation of microdisk electrode unit, microalgae recovery unit includes membrane filter system, the permeate delivery outlet of membrane filter system is connected to make the permeate of output by reuse to microdisk electrode unit, molecular cut off≤30kDa of the filter membrane of membrane filter system with the feed inlet of microdisk electrode unit.The present invention realizes recycling for breeding water body.
Description
Technical field
The present invention relates to field of microalgae cultivation, and in particular to a kind of method and system of Cyclic culture microalgae.
Background technique
" energy " and " environment " is the major issue that new century human society in sustainable development is faced, on the one hand,
The fossil energy of support mankind's modern civilization is non-renewable, thus countries in the world are stepping up to develop alternative energy source technology；Separately
On the one hand, the mankind are inevitably generated the emission problem of serious exhaust gas and sewage when processing and using fossil energy, right
The living environment of weather and the mankind have had resulted in serious influence.These problems need the solution made overall plans and coordinate.
Microalgae is " chemical plant living " of ultrahigh in efficiency driven by sunlight, by the efficient photosynthesis of microalgae cell,
The chemical energy of the carbohydrate such as fat or starch is converted light energy into, and releases O2.Using microalgae production bioenergy and change
Product can reach the dual purpose of " substitution fossil energy and reduction industrial gas emission " simultaneously.Microalgae is a kind of very original
Living resources, without Organ Differentiation, so have the characteristics that growth is fast, yield is high, environmental suitability is strong, and with high plant
Object is compared, and the content of the effective components such as their lipid, starch and protein is higher.The traditional commerce application of microalgae includes by it
Be used as food additives, the feed in agricultural and culture fishery and chemical industry raw material.In addition, certain algae tools
Standby oil content height is easy to the advantages that culture, yield per unit area do not strive ground greatly, with agricultural, is considered as a new generation, even only
One is able to achieve the biodiesel raw material of substitute fossil fuels.
In microalga cultivation process, the harvesting of microalgae generallys use centrifugation or flucculation process, but such method energy consumption
It is higher, it is unfavorable for realizing green production.In addition, the harvesting of microalgae and recycling for breeding water body are to influence microalgae cyclic culture
The key factor of success or failure is directly returned by the remaining breeding water body after centrifugation or flocculated mode microalgae under normal circumstances
With the microalgae yield (stability is weaker) that can be seriously affected to microdisk electrode process in subsequent process, therefore generally it is difficult to ensuring
Recycling for breeding water body is realized while microalgae yield.
Using film filter can particle, colloid and organic matter etc. in effectively catching water, be widely used in food, light textile,
The industries such as chemical industry, medicine and environmentally friendly electronics.But general film filtering uses inner pressed filter type, membrane filter system is being transported
Filter membrane is easy to appear blocking after row a period of time, is backwashed, this not only makes process become complicated, can also significantly increase
Add energy consumption.
Summary of the invention
The purpose of the invention is to overcome microdisk electrode energy consumption height of the existing technology and be difficult to recycle cultivation
The problem of water body, provides a kind of method that energy consumption reduces ground Cyclic culture microalgae.
It was found by the inventors of the present invention that the filter membrane of specific molecular cut off is selected to flow through micro algae culturing liquid with continuous circulation
The mode of filter membrane, which carries out film filtering, can be achieved at the same time recycling for breeding water body, and relative to conventional centrifuging process energy consumption
Lower, filter membrane is also not easy to plug.Therefore.To achieve the goals above, one aspect of the present invention provides a kind of Cyclic culture microalgae
Method, this method comprises:
(1) microalgae is cultivated, obtains micro algae culturing liquid；
(2) micro algae culturing liquid of step (1) harvest is recycled in the circuit for being equipped with membrane filter system, microdisk electrode liquid stream
When through filter membrane in membrane filter system, the partially liq in micro algae culturing liquid penetrates filter membrane, to respectively obtain in filter membrane two sides
The algae solution of permeate and concentration；By the permeate reuse to step (1)；Wherein, molecular cut off≤30kDa of filter membrane.
Second aspect of the present invention provides a kind of system of Cyclic culture microalgae, which includes microdisk electrode unit and use
In the microalgae recovery unit for collecting the microalgae that microdisk electrode unit generates, wherein the microalgae recovery unit includes that film filtering is set
Standby and optional drying equipment, the membrane filter system include water-impermeable hard cavity and are set to the intracorporal filter membrane of chamber；
The cavity is provided with inlet port and outlet port and algae solution is cyclically introduced into the cavity, and algae solution flows through filter membrane
(1) when, the partially liq in micro algae culturing liquid penetrates filter membrane, to respectively obtain the algae of permeate and concentration in filter membrane two sides
Liquid；The discharge port of the microalgae recovery unit is connected with the feed inlet of microdisk electrode unit, so that microalgae recovery unit obtained
Permeate can be by reuse to microdisk electrode unit, molecular cut off≤30kDa of the filter membrane.
By above technical scheme, the present invention realizes recycling for breeding water body while guaranteeing microalgae yield,
Stability is strong, has largely saved energy consumption, reduces the yield of waste water, realizes the green production of microalgae.
Detailed description of the invention
Fig. 1 is the growth curve of chlorella in embodiment 1 and embodiment 4；
Fig. 2 is the growth curve of chlorella in comparative example 1 and comparative example 2；
Fig. 3 is the growth curve of scenedesmus in embodiment 2 and embodiment 5；
Fig. 4 is the growth curve of scenedesmus in comparative example 3 and comparative example 4；
Fig. 5 is the growth curve of chlorella in embodiment 3.
Specific embodiment
The endpoint of disclosed range and any value are not limited to the accurate range or value herein, these ranges or
Value should be understood as comprising the value close to these ranges or value.For numberical range, between the endpoint value of each range, respectively
It can be combined with each other between the endpoint value of a range and individual point value, and individually between point value and obtain one or more
New numberical range, these numberical ranges should be considered as specific open herein.
The method of Cyclic culture microalgae provided by the invention includes:
(1) microalgae is cultivated, obtains micro algae culturing liquid；
(2) micro algae culturing liquid of step (1) harvest is recycled in the circuit for being equipped with membrane filter system, microdisk electrode liquid stream
When through filter membrane in membrane filter system, the partially liq in micro algae culturing liquid penetrates filter membrane, to respectively obtain in filter membrane two sides
The algae solution of permeate and concentration；By the permeate reuse to step (1)；Wherein, molecular cut off≤30kDa of filter membrane, it is excellent
It is selected as 5-30kDa, more preferably 30kDa.Wherein, circulation refers to micro algae culturing liquid with not in the circuit for being equipped with membrane filter system
Intermittent mode carries out film filtering, and the algae solution being concentrated carries out film filtering through filter membrane with fresh microdisk electrode liquid recycle stream again,
The circuit of circulation is formed between micro algae culturing liquid and filter membrane.
In the present invention, conventional culture medium can be used and carry out microdisk electrode, for example, BG-11 culture medium, SE culture medium,
At least one of Pr culture medium, f/2 culture medium, Zarrouk culture medium (preferably BG-11 culture medium).
It is well known by those skilled in the art that can be mended into cultivating system to obtain further amounts of microalgae biomass
Add nitrogen source (and other nutriments-are especially carbon source).Therefore, a preferred embodiment of the invention, the method
It further include nitrogen source (such as NO monitored in cultivating system during the cultivation process3 -And/or NO2 -) content, thus in due course toward cultivating system
Add nitrogen source (such as NO3 -And/or NO2 -).When using nitric acid as nitrogen source, the amount for the nitric acid added makes the pH value of cultivating system
It maintains in 6.5-10 (preferably 7-9.5) range.The additional amount of carbon source (such as glucose) usually makes in cultivating system carbon source
Content is within the scope of 5-10g/L.
In the present invention, the culture can carry out under the conditions of conventional optical-biological reaction, to temperature, illumination, pH, dissolved oxygen
Amount etc. does not require particularly, can be adjusted according to the type of used microalgae.Normally, the condition of culture includes:
Temperature is 15-40 DEG C, intensity of illumination 1000-200000lux, and pH value is 6.5-10 (preferably 7-9.5), ventilatory capacity 0.5-
1.5vvm.Wherein, " vvm " refers to the ratio of the volume of minute ventilation volume (standard state) and cultivating system.
In the present invention, before being cultivated, it is terrible for being to the algae of microalgae progress seed culture, seed culture
To more pure and strong microalgae, that is, is flushed, is inoculated with the enough algaes of quantity.The side of this field routine can be used
Method carries out seed culture, and details are not described herein.
During microdisk electrode, in order to obtain higher microalgae yield, can be added into cultivating system antibiotic with
Control the growth of miscellaneous bacteria.The antibiotic can be antibiotic commonly used in the art, for example, benzyl penicillin, ammonia can be selected from
Parasiticin, carbenicillin, streptomysin, gentamicin, kanamycins, neomycin, chloramphenicol, erythromycin, tetracycline, soil are mould
At least one of element, nalidixic acid and rifampin.
In the present invention, the various common microalgaes in this field can be selected to be cultivated, for example, can be cyanobacteria or green alga.
Under preferable case, the microalgae is chlorella (such as chlorella (Chlorella vulgaris), chlorella ellipsoidea
(C.ellipsoidea) or chlorella pyrenoidosa (C.pyrenoidosa)), single needle algae (such as Dai Shi single needle algae
(Monoraphidiumdybowskii)), scenedesmus (such as scenedesmus obliquus (Scenedesmusobliqnus), tapering scenedesmus
(S.acuminatus), Scenedesmus arcuatus (S.arcuatus), by first scenedesmus (S.armatus) or Scenedesmus quadricauda
Or spirulina (such as blunt top spirulina (Spirulina platensis)) (S.quadricauda)).
It, can be according to the culture of micro algae growth state and existing micro-judgment step (1) in cultivating system in the present invention
Terminal, and subsequent operation is carried out, it repeats no more.
In the present invention, the circulation of both culturing microalgae water body can be realized by the way that at least partly micro algae culturing liquid is carried out film filtering
It utilizes, the amount for the micro algae culturing liquid for carrying out film filtering is not required particularly, but in a preferred embodiment of the invention, step
Suddenly in (1), at the end of culture, the microdisk electrode that part micro algae culturing liquid carries out next round as algae, remaining microalgae are left
Culture solution carries out step (2).It is highly preferred that the micro algae culturing liquid for leaving 10-20 volume % carries out the micro- of next round as algae
Algae culture, remaining (80-90 volume %) micro algae culturing liquid carry out step (2).The remaining micro algae culturing liquid of film filtering is not carried out
(as algae) can together with permeate return step (1).
In the present invention, it can be organic film that film, which filters used filter membrane, or inoranic membrane.Film filtering can be normal
It is carried out under the pressure of rule, for example, the inlet pressure of membrane filter system is 0.05- in a kind of specific embodiment
0.15MPa, outlet pressure 0.03-0.06MPa.
In the present invention, film filtering gained trapped substance (algae solution of concentration) is through simple dry (such as spray drying) step
Microalgae biomass product can be obtained.Microalgae content (>=80g/L) in gained trapped substance (algae solution of concentration) is being adapted for spraying
In the dry range of mist, therefore, can directly it be spray-dried after film filters without carrying out other operations, process simplification.
Spray drying can be realized by spray dryer.
So that micro algae culturing liquid is continuously circulated through filter membrane and is avoided as much as material in the deposition of filter membrane surface, from
And prevent filter membrane from blocking.In a preferred embodiment of the invention, in order to realize being carried out continuously for film filtering, micro algae culturing liquid exists
The mode recycled in circuit equipped with membrane filter system are as follows: at the end of the culture of step (1), the micro algae culturing liquid of harvest is drawn
Enter liquid storage facility (fluid reservoir)；It recycles micro algae culturing liquid between membrane filter system and liquid storage facility, controls filter membrane two
The pressure difference of side is 0.03-0.15MPa, makes the partially liq in micro algae culturing liquid through filter membrane, respectively obtains in filter membrane two sides
The algae solution of permeate and concentration.
The film filtering carries out on membrane filter system, and the filter membrane is preferably hollow-fibre membrane.Generally, the film mistake
Filter equipment may include: water-impermeable hard cavity and be set to the intracorporal hollow-fibre membrane of chamber.Film filtering can be with
By liquid storage facility, micro algae culturing liquid circulates in hollow-fibre membrane and liquid storage facility, and passes through hollow-fibre membrane both ends
Pressure official post partially liq flow out to obtain permeate through hollow-fibre membrane.The micro algae culturing liquid can by circulating pump into
Enter membrane filter system and flows through hollow-fibre membrane.
Wherein, the cavity can be made by various conventional water-impermeable hard materials, such as plastics, stainless steel.
In a preferred embodiment, the cavity is arranged in such a way that filter membrane is perpendicular to horizontal plane, so that filter membrane is less
Easily it is blocked.
The system of Cyclic culture microalgae provided by the invention includes microdisk electrode unit and for collecting microdisk electrode unit
The microalgae recovery unit of the microalgae of generation, which is characterized in that the microalgae recovery unit includes that membrane filter system is done with optional
Dry equipment, the membrane filter system include water-impermeable hard cavity and are set to the intracorporal filter membrane of chamber；The cavity setting
There are inlet port and outlet port that algae solution is cyclically introduced into the cavity, and when algae solution flows through filter membrane, microdisk electrode
Partially liq in liquid penetrates filter membrane, to respectively obtain the algae solution of permeate and concentration in filter membrane two sides；The microalgae recovery
The discharge port of unit is connected with the feed inlet of microdisk electrode unit, and the permeate that microalgae recovery unit is obtained is by reuse
To microdisk electrode unit, molecular cut off≤30kDa of the filter membrane, preferably 5-30kDa, more preferably 30kDa.
According to the present invention, the microalgae recovery unit is the unit of microalgae, and by the present invention in that being set with film filtering
The standby main device as harvesting can be realized recycling and saving the required energy consumption of harvesting for both culturing microalgae water body.The film
Filter plant can be the common various equipment in this field, as long as it is above-mentioned to meet the molecular cut off of the membrane module wherein configured
It is required that and algae solution filter membrane can be flowed through in a manner of continuously recycling.The drying equipment can be commonly used in the art
Drying equipment, such as spray drying device.
According to the preferred embodiment of the present invention, the microalgae recovery unit further includes liquid storage facility and/or for controlling
The inlet port and outlet port of the pressure controlling equipment of both sides differential pressure of membrane, water-impermeable hard cavity are connected with liquid storage facility respectively
To form the circuit of circulation.As previously mentioned, the micro algae culturing liquid of harvest is introduced liquid storage facility；Then micro algae culturing liquid is made to exist
It is recycled between membrane filter system and liquid storage facility, controls the pressure difference of filter membrane two sides, keep the partially liq in micro algae culturing liquid saturating
Filter membrane respectively obtains the algae solution of permeate and concentration in filter membrane two sides.The micro algae culturing liquid can enter by circulating pump
Membrane filter system flows through filter membrane.
Wherein, the cavity can be made by various conventional water-impermeable hard materials, such as plastics, stainless steel.
As previously mentioned, in a preferred embodiment, the cavity is arranged in such a way that filter membrane is perpendicular to horizontal plane, so that
Filter membrane is less susceptible to be blocked.The filter membrane is preferably hollow-fibre membrane.
The present invention will be described in detail by way of examples below.
Algae solution OD value (OD680) measurement: OD value spectrophotometric determination is compared with distilled water, measures algae
Light absorption value of the liquid at wavelength 680nm, the index as frustule concentration.
BG11 culture medium: nitrogen source is NaNO in BG11 culture medium when initial incubation3, HNO is added in breeding process3As nitrogen
Source.
Membrane filter unit include: water-impermeable hard cavity (tubular body being disposed vertically), be set to chamber it is intracorporal hang down
Directly in the filter membrane of horizontal plane (hollow-fibre membrane) and liquid storage facility (fluid reservoir), the cavity, which is provided with inlet port and outlet port, to be made
Algae solution can be cyclically introduced into the cavity, and when algae solution flows through filter membrane, the partially liq in micro algae culturing liquid is penetrated
Filter membrane, to respectively obtain the algae solution of permeate and concentration in filter membrane two sides；The discharge port and microalgae of the microalgae recovery unit
The feed inlet for cultivating unit is connected, and the permeate that microalgae recovery unit is obtained is by reuse to microdisk electrode unit；Film
The concrete mode of filtering are as follows: the micro algae culturing liquid of harvest is introduced into liquid storage facility；Then make micro algae culturing liquid in membrane filter system
It is recycled between liquid storage facility, controls the pressure difference of filter membrane two sides, made the partially liq in micro algae culturing liquid through filter membrane, filtering
Film two sides respectively obtain the algae solution of permeate and concentration.
Spray drying is implemented by YC-015 experiment type spray drier, condition are as follows: and 200 DEG C of inlet air temperature, leaving air temp
80 DEG C, sample introduction speed is 80mL/h.
The ingredient of BG11 culture medium is as follows:
Microelement A5 contains following ingredient:
Embodiment 1
Chlorella algae is purchased from Inst. of Hydrobiology, Chinese Academy of Sciences, BG11 culture medium culture.Algae is in the Portugal containing 5g/L
2d is induced in the BG11 culture medium of grape sugar, then access sterile in the illumination fermentation tank of the culture medium of BG11 containing 3L of 5L and is supported
It grows, algae solution OD after inoculation680Control is 1 or so, cultivating condition are as follows: 28 DEG C of temperature, ventilatory capacity about 0.9vvm, speed of agitator are about
280rpm, intensity of illumination 6000lux, light dark period 12h:12h are added antibiotic and control bacterial growth (kanamycins 50mg/L
With chloramphenicol 10mg/L), and be added 0.1 volume % defoaming agent (silicone emulsion).In breeding process, to cell density (with
Absorbance OD at 680nm680For index) it is monitored, nutritive salt is added according to the growth conditions of microalgae, to control grape
Sugared concentration is 5-10g/L, and the nitrogen source added is HNO3, HNO3Additional amount makes algae solution pH control between 7.5-9.After cultivating 3d
Taking out 90 volume % algae solutions, (the microalgae content in terms of dry weight enters membrane filter unit for 22.5g/L).
The inlet pressure 0.1MPa of membrane filter unit, outlet pressure 0.05MPa, the molecular cut off 30kDa of film, after filtering
Obtaining permeate and algae solution concentrate, (the microalgae content in terms of dry weight is 97.5g/L), permeate direct reuse, and concentrate carries out
Spray drying obtains microalgae product.
Permeate is added directly into 5L fermentor, using the remaining 10 volume % algae solution of previous sample as algae, Replacement Battalion
Continue to cultivate after supporting salt, antibiotic and defoaming agent, wherein nitrogen source is HNO3, cultivating condition is same as above, according to microalgae in breeding process
Growing state extra-nutrition salt and HNO3, after cultivating 3d, take out 90 volume % algae solutions and enter membrane filter unit, repeat above-mentioned step
Suddenly.
Chlorella growth curve is as shown in Figure 1.
Comparative example 1
Chlorella cultivating condition is with embodiment 1, except that algae solution is in fermentor after taking out, aseptically
Centrifugation, 4500r/min are centrifuged 15min, and then supernatant, which is refunded in fermentor, continues both culturing microalgae experiment.Chlorella growth curve
As shown in Figure 2.
Comparative example 2
Chlorella cultivating condition is with embodiment 1, except that the molecular cut off of film is 40kDa, chlorella growth is bent
Line is as shown in Figure 2.Film filtering is carried out after cultivating for the first time, microalgae content of the algae solution in terms of dry weight is 19.4g/L, film filtering
Microalgae content of the concentrate afterwards in terms of dry weight is 100.8g/L.
Embodiment 2
Scenedesmus is purchased from Inst. of Hydrobiology, Chinese Academy of Sciences, BG11 culture medium culture.3L is added in 5L triangular flask
BG11 culture medium, is then inoculated with, OD after inoculation680Control is about 0.8, cultivating condition are as follows: 28 DEG C of temperature, ventilatory capacity 0.8-1vvm,
Light intensity 6000lux, light dark period 12h:12h, in breeding process, to cell density (with the absorbance OD at 680nm680To refer to
Mark) it is monitored, supplement HNO3With other nutritive salt, pH is controlled between 7-8, and the algae solution of 80 volume % is taken out after cultivation 7d
(the microalgae content in terms of dry weight is 1.2g/L) carries out film filtering.Membrane filter unit inlet pressure 0.1MPa, outlet pressure
0.05MPa, the molecular cut off 30kDa of film.The filtered concentrate of film (the microalgae content in terms of dry weight is 87g/L) is sprayed
Mist is dry, obtains microalgae product.It will transmit through liquid to refund in triangular flask and to sample remaining algae solution (20 volume %) as algae continuation
It cultivates, according to the growing state extra-nutrition salt and HNO of microalgae in breeding process3, repeat aforesaid operations.
Scenedesmus growth curve is as shown in Figure 3.
Comparative example 3
Scenedesmus cultivating condition is with embodiment 2, except that algae solution is in fermentor after taking out, aseptically from
The heart is centrifuged 15min in 4500r/min, and then supernatant, which is refunded in fermentor, continues both culturing microalgae experiment.The growth curve of scenedesmus
As shown in Figure 4.
Comparative example 4
Scenedesmus cultivating condition is with embodiment 2, except that the molecular cut off of film is 40kDa, scenedesmus growth curve is such as
Shown in Fig. 4, film filtering is carried out after cultivating for the first time, microalgae content of the algae solution in terms of dry weight is 1.4g/L, and film is filtered
Microalgae content of the concentrate in terms of dry weight is 98.3g/L.
Embodiment 3
The culture chlorella in the way of embodiment 1, unlike, take the algae solution (microalgae in terms of dry weight of 70 volume %
Content is 19.7g/L) enter membrane filter unit.Microalgae content of the filtered concentrate of film in terms of dry weight is 87.9g/L, bead
Algae growth curve is as shown in Figure 5.
Embodiment 4
Chlorella cultivating condition is with embodiment 1, except that adding KNO in microalga cultivation process3It is small as nitrogen source
As shown in Figure 1, carrying out film filtering after cultivating for the first time, microalgae content of the algae solution in terms of dry weight is ball algae growth curve
22.6g/L, microalgae content of the filtered concentrate of film in terms of dry weight is 107.4g/L.
Embodiment 5
Scenedesmus cultivating condition is with embodiment 2, except that adding KNO in microalga cultivation process3As nitrogen source, scenedesmus
Growth curve is as shown in figure 3, carrying out microalgae content of the algae solution of film filtering in terms of dry weight is 1.3g/L, the filtered concentrate of film
Microalgae content in terms of dry weight is 94.8g/L.
The energy consumption of cultural method of the invention and conventional centrifugal method is counted it can be concluded that, method of the invention
Energy consumption be only centrifugal method half or lower, therefore method of the invention has the advantage that low energy consumption.
By result above as can be seen that using the method for the present invention high yield can be obtained in the lower situation of energy consumption
Microalgae biomass.Moreover, the present invention still is able to obtain higher microalgae yield after circulation 300h (carrying out 4 circulations),
Illustrate still to be able to realize steady production, and filter membrane still is able to effectively realize that film filters, and does not block up even if water circulation will be cultivated
Plug.
The preferred embodiment of the present invention has been described above in detail, and still, the present invention is not limited thereto.In skill of the invention
In art conception range, can with various simple variants of the technical solution of the present invention are made, including each technical characteristic with it is any its
Its suitable method is combined, and it should also be regarded as the disclosure of the present invention for these simple variants and combination, is belonged to
Protection scope of the present invention.
Claims (10)
1. a kind of method of Cyclic culture microalgae, which is characterized in that this method comprises:
(1) microalgae is cultivated, obtains micro algae culturing liquid；
(2) micro algae culturing liquid of step (1) harvest is recycled in the circuit for being equipped with membrane filter system, micro algae culturing liquid flows through film
When filter membrane in filter plant, the partially liq in micro algae culturing liquid penetrates filter membrane, to respectively obtain transmission in filter membrane two sides
The algae solution of liquid and concentration；By the permeate reuse to step (1)；Wherein, molecular cut off≤30kDa of filter membrane.
2. according to the method described in claim 1, wherein, the condition of culture includes: that temperature is 15-40 DEG C, and intensity of illumination is
1000-200000lux, pH value 6.5-10, ventilatory capacity 0.5-1.5vvm.
3. method according to claim 1 or 2, wherein the microalgae is cyanobacteria or green alga, preferably chlorella, single needle
Algae, scenedesmus or spirulina.
4. according to the method described in claim 1, wherein, in step (1), at the end of culture, leaving part micro algae culturing liquid work
The microdisk electrode of next round is carried out for algae, remaining micro algae culturing liquid carries out step (2)；Preferably, 10-20 volume % is left
Micro algae culturing liquid as algae carry out next round microdisk electrode, remaining micro algae culturing liquid carry out step (2).
5. according to the method described in claim 1, wherein, the micro algae culturing liquid recycles in the circuit for being equipped with membrane filter system
Mode are as follows: at the end of the culture of step (1), the micro algae culturing liquid of harvest is introduced into liquid storage facility；Then make microdisk electrode
Liquid recycles between membrane filter system and liquid storage facility, and the pressure difference of control filter membrane two sides is 0.03-0.15MPa, trains microalgae
Partially liq in nutrient solution penetrates filter membrane, respectively obtains the algae solution of permeate and concentration in filter membrane two sides.
6. method according to claim 1 or 5, wherein the filter membrane is hollow-fibre membrane.
7. a kind of system of Cyclic culture microalgae, which includes microdisk electrode unit and generates for collecting microdisk electrode unit
Microalgae microalgae recovery unit, which is characterized in that the microalgae recovery unit includes that membrane filter system and optional drying are set
Standby, the membrane filter system includes water-impermeable hard cavity and is set to the intracorporal filter membrane of chamber；The cavity be provided with into
Material mouth and discharge port enable algae solution to be cyclically introduced into the cavity, and when algae solution flows through filter membrane, in micro algae culturing liquid
Partially liq penetrate filter membrane, to respectively obtain the algae solution of permeate and concentration in filter membrane two sides；The microalgae recovery unit
Discharge port be connected with the feed inlet of microdisk electrode unit, the permeate that microalgae recovery unit is obtained is by reuse to micro-
Algae cultivates unit, molecular cut off≤30kDa of the filter membrane.
8. system according to claim 7, wherein the microalgae recovery unit further includes liquid storage facility and/or for controlling
The pressure controlling equipment of both sides differential pressure of membrane processed, the inlet port and outlet port of water-impermeable hard cavity respectively with liquid storage facility phase
Even to form the circuit of circulation.
9. system according to claim 7 or 8, wherein the cavity is arranged in such a way that filter membrane is perpendicular to horizontal plane.
10. system according to claim 7 or 8, wherein the filter membrane is hollow-fibre membrane.
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| CN112811479A (en) * | 2020-12-29 | 2021-05-18 | 同济大学 | Whole-process optimization method for recycling microalgae culture wastewater |
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