CN110066737A - Microalgae and the method for obtaining microalgae biomass - Google Patents
Microalgae and the method for obtaining microalgae biomass Download PDFInfo
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Abstract
The present invention relates to microalgae recovery field, the method for disclosing microalgae and obtaining microalgae biomass.The method of microalgae includes the steps that being concentrated using micro algae culturing liquid of the membrane filter system to harvest: in the step, the micro algae culturing liquid of harvest is recycled in the circuit for being equipped with membrane filter system, when micro algae culturing liquid flows through the filter membrane in membrane filter system, partially liq in micro algae culturing liquid penetrates filter membrane, respectively obtains the algae solution of permeate and concentration in filter membrane two sides.Second aspect of the present invention discloses a kind of method for obtaining microalgae biomass, includes the steps that cultivating microalgae, microalgae and extracts microalgae biomass, which is characterized in that uses method microalgae as described above.By above technical scheme, the present invention can effective microalgae, stability is strong, has largely saved energy consumption, reduces the yield of waste water, realize microalgae green harvesting.
Description
Technical field
The present invention relates to microalgae recovery fields, and in particular to a kind of method of microalgae and acquisition microalgae biomass.
Background technique
" energy " and " environment " is the major issue that new century human society in sustainable development is faced, on the one hand,
The fossil energy of support mankind's modern civilization is non-renewable, thus countries in the world are stepping up to develop alternative energy source technology；Separately
On the one hand, the mankind are inevitably generated the emission problem of serious exhaust gas and sewage when processing and using fossil energy, right
The living environment of weather and the mankind have had resulted in serious influence.These problems need the solution made overall plans and coordinate.
Microalgae is " chemical plant living " of ultrahigh in efficiency driven by sunlight, by the efficient photosynthesis of microalgae cell,
The chemical energy of the carbohydrate such as fat or starch is converted light energy into, and releases O2.Using microalgae production bioenergy and change
Product can reach the dual purpose of " substitution fossil energy and reduction industrial gas emission " simultaneously.Microalgae is a kind of very original
Living resources, without Organ Differentiation, so have the characteristics that growth is fast, yield is high, environmental suitability is strong, and with high plant
Object is compared, and the content of the effective components such as their lipid, starch and protein is higher.The traditional commerce application of microalgae includes by it
Be used as food additives, the feed in agricultural and culture fishery and chemical industry raw material.In addition, certain algae tools
Standby oil content height is easy to the advantages that culture, yield per unit area do not strive ground greatly, with agricultural, is considered as a new generation, even only
One is able to achieve the biodiesel raw material of substitute fossil fuels.
In microalga cultivation process, the harvesting of microalgae generallys use centrifugation or flucculation process, but such method energy consumption
It is higher, it is unfavorable for realizing green production.In addition, the harvesting of microalgae and recycling for breeding water body are to influence microalgae cyclic culture
The key factor of success or failure is directly returned by the remaining breeding water body after centrifugation or flocculated mode microalgae under normal circumstances
With the microalgae yield (stability is weaker) that can be seriously affected to microdisk electrode process in subsequent process, therefore generally it is difficult to ensuring
Recycling for breeding water body is realized while microalgae yield.
Using film filter can particle, colloid and organic matter etc. in effectively catching water, be widely used in food, light textile,
The industries such as chemical industry, medicine and environmentally friendly electronics.But general film filtering uses inner pressed filter type, membrane filter system is being transported
Filter membrane is easy to appear blocking after row a period of time, is backwashed, this not only makes process become complicated, can also significantly increase
Add energy consumption.
Summary of the invention
The purpose of the invention is to overcome microalgae recovery energy consumption height of the existing technology and be difficult to recycle cultivation
The problem of water body, provides a kind of method that energy consumption reduces ground microalgae and obtains microalgae biomass.
It was found by the inventors of the present invention that energy consumption is lower in a manner of recycling and flow through filter membrane to micro algae culturing liquid, filter membrane is not yet
Easily blocking.Therefore.To achieve the goals above, one aspect of the present invention provides a kind of method of microalgae, and this method includes
The step of being concentrated using micro algae culturing liquid of the membrane filter system to harvest: in the step, the micro algae culturing liquid of harvest is existed
It is recycled in circuit equipped with membrane filter system, when micro algae culturing liquid flows through the filter membrane in membrane filter system, in micro algae culturing liquid
Partially liq penetrates filter membrane, respectively obtains the algae solution of permeate and concentration in filter membrane two sides.
Second aspect of the present invention provide it is a kind of obtain microalgae biomass method, including culture microalgae, microalgae and
The step of extracting microalgae biomass, which is characterized in that use method microalgae as described above.
By above technical scheme, the present invention can effective microalgae, stability is strong, has largely saved energy
Consumption, reduces the yield of waste water, realizes the green harvesting of microalgae.Moreover, in a preferred embodiment, can also protect
Recycling for breeding water body is realized while demonstrate,proving microalgae yield.
Detailed description of the invention
Fig. 1 is the growth curve of chlorella in embodiment 1 and embodiment 4；
Fig. 2 is the growth curve of chlorella in comparative example 1 and embodiment 6；
Fig. 3 is the growth curve of scenedesmus in embodiment 2 and embodiment 5；
Fig. 4 is the growth curve of scenedesmus in comparative example 2 and embodiment 7；
Fig. 5 is the growth curve of chlorella in embodiment 3.
Specific embodiment
The endpoint of disclosed range and any value are not limited to the accurate range or value herein, these ranges or
Value should be understood as comprising the value close to these ranges or value.For numberical range, between the endpoint value of each range, respectively
It can be combined with each other between the endpoint value of a range and individual point value, and individually between point value and obtain one or more
New numberical range, these numberical ranges should be considered as specific open herein.
The method of microalgae provided by the invention includes dense using micro algae culturing liquid progress of the membrane filter system to harvest
The step of contracting: in the step, the micro algae culturing liquid of harvest is recycled in the circuit for being equipped with membrane filter system, microdisk electrode liquid stream
When through filter membrane in membrane filter system, the partially liq in micro algae culturing liquid penetrates filter membrane, respectively obtains transmission in filter membrane two sides
The algae solution of liquid and concentration.
In the present invention, at the end of the concentration step, the microalgae content in the algae solution of concentration in terms of dry weight is preferably 80-
150g/L。
In the present invention, the method can also include being spray-dried to the micro algae culturing liquid of concentration, be done with acquisition micro-
The step of algae.
In the present invention, molecular cut off (or aperture) size of the filter membrane is not particularly limited, as long as being less than micro-
The size of frustule (or cell fragment), for example, the molecular cut off of the filter membrane can with≤300kDa, preferably≤
30kDa, more preferably 5-30kDa, most preferably 30kDa.The filter membrane of specific molecular cut off (≤30kDa) is selected to train microalgae
Nutrient solution, which carries out film filtering in such a way that continuous circulation flows through filter membrane, can be achieved at the same time recycling for breeding water body, namely penetrate
(therefore, method of the invention can also include by the permeate reuse to microalgae to the step of liquid can be with reuse to culture microalgae
Incubation step), and it is lower relative to conventional centrifuging process energy consumption, filter membrane is also not easy to plug.
The mode of the concentration are as follows: the micro algae culturing liquid of harvest is recycled between membrane filter system and liquid storage facility, is controlled
The pressure difference of filter membrane two sides processed is 0.02-0.05MPa, makes the partially liq in micro algae culturing liquid through filter membrane, in filter membrane two sides
Respectively obtain the algae solution of permeate and concentration.
In the present invention, it can be organic film that film, which filters used filter membrane, or inoranic membrane, preferably doughnut
Film.
The method provided by the invention for obtaining microalgae biomass includes culture microalgae, microalgae and extracts microalgae biomass
The step of, which is characterized in that use method microalgae as described above.
In the present invention, conventional culture medium can be used and carry out microdisk electrode, for example, BG-11 culture medium, SE culture medium,
At least one of Pr culture medium, f/2 culture medium, Zarrouk culture medium (preferably BG-11 culture medium).
It is well known by those skilled in the art that can be mended into cultivating system to obtain further amounts of microalgae biomass
Add nitrogen source (and other nutriments-are especially carbon source).Therefore, a preferred embodiment of the invention, the method
It further include nitrogen source (such as NO monitored in cultivating system during the cultivation process3 -And/or NO2 -) content, thus in due course toward cultivating system
Add nitrogen source (such as NO3 -And/or NO2 -).When using nitric acid as nitrogen source, the amount for the nitric acid added makes the pH value of cultivating system
It maintains in 6.5-10 (preferably 7-9.5) range.The additional amount of carbon source (such as glucose) usually makes in cultivating system carbon source
Content is within the scope of 5-10g/L.
According to a kind of specific embodiment, the method can also include by the NO in flue gasxIt is converted into NO3 -And/or
NO2 -, and using it as incubation step in cultivate microalgae nitrogen source.
In the present invention, microdisk electrode can carry out under the conditions of conventional optical-biological reaction, to temperature, illumination, pH, dissolved oxygen
Amount etc. does not require particularly, can be adjusted according to the type of used microalgae.Normally, the condition of culture includes:
Temperature is 15-40 DEG C, intensity of illumination 1000-200000lux, and pH value is 6.5-10 (preferably 7-9.5), ventilatory capacity 0.5-
1.5vvm.Wherein, " vvm " refers to the ratio of the volume of minute ventilation volume (standard state) and cultivating system.
In the present invention, before carrying out microdisk electrode, can to the algae of microalgae carry out seed culture, seed culture be for
More pure and strong microalgae is obtained, that is, flushed, be inoculated with the enough algaes of quantity.It can be conventional using this field
Method carry out seed culture, details are not described herein.
During microdisk electrode, in order to obtain higher microalgae yield, can be added into cultivating system antibiotic with
Control the growth of miscellaneous bacteria.The antibiotic can be antibiotic commonly used in the art, for example, benzyl penicillin, ammonia can be selected from
Parasiticin, carbenicillin, streptomysin, gentamicin, kanamycins, neomycin, chloramphenicol, erythromycin, tetracycline, soil are mould
At least one of element, nalidixic acid and rifampin.
In the present invention, the various common microalgaes in this field can be selected to be cultivated, as long as can be with NO3 -And/or NO2 -
As nitrogen source, for example, can be cyanobacteria or green alga.Under preferable case, the microalgae is chlorella (such as chlorella
(Chlorella vulgaris), chlorella ellipsoidea (C.ellipsoidea) or chlorella pyrenoidosa (C.pyrenoidosa)),
Single needle algae (such as Dai Shi single needle algae (Monoraphidiumdybowskii)), scenedesmus (such as scenedesmus obliquus
(Scenedesmusobliqnus), tapering scenedesmus (S.acuminatus), Scenedesmus arcuatus (S.arcuatus), by first scenedesmus
(S.armatus) or Scenedesmus quadricauda (S.quadricauda)) or spirulina (such as blunt top spirulina (Spirulina
platensis))。
It, can be according to the culture of micro algae growth state and existing micro-judgment microdisk electrode in cultivating system in the present invention
Terminal, and subsequent operation is carried out, it repeats no more.
In the present invention, following for both culturing microalgae water body is advantageously implemented by the way that at least partly micro algae culturing liquid is carried out film filtering
Ring utilizes, and does not require particularly the amount for the micro algae culturing liquid for carrying out film filtering, but in a preferred embodiment of the invention,
At the end of microdisk electrode, the microdisk electrode that part micro algae culturing liquid carries out next round as algae, remaining microdisk electrode are left
Liquid carries out harvesting step.It is highly preferred that the micro algae culturing liquid for leaving 10-20 volume % carries out the microalgae training of next round as algae
It supports, remaining (80-90 volume %) micro algae culturing liquid carries out harvesting step.The remaining micro algae culturing liquid for not carrying out film filtering (is made
For algae) incubation step can be returned together with permeate.
In the present invention, the method for extracting microalgae biomass can carry out according to conventional methods in the art, microalgae biomass
Generally include grease, albumen etc..
The present invention will be described in detail by way of examples below.
Algae solution OD value (OD680) measurement: OD value spectrophotometric determination is compared with distilled water, measures algae
Light absorption value of the liquid at wavelength 680nm, the index as frustule concentration.
BG11 culture medium: nitrogen source is NaNO in BG11 culture medium when initial incubation3, HNO is added in breeding process3As nitrogen
Source.
Membrane filter unit include: water-impermeable hard cavity (tubular body being disposed vertically), be set to chamber it is intracorporal hang down
Directly in the filter membrane of horizontal plane (hollow-fibre membrane) and liquid storage facility (fluid reservoir), the cavity, which is provided with inlet port and outlet port, to be made
Algae solution can be cyclically introduced into the cavity, and when algae solution flows through filter membrane, the partially liq in micro algae culturing liquid is penetrated
Filter membrane, to respectively obtain the algae solution of permeate and concentration in filter membrane two sides；The discharge port and microalgae of the microalgae recovery unit
The feed inlet for cultivating unit is connected, and the permeate that microalgae recovery unit is obtained is by reuse to microdisk electrode unit；Film
The concrete mode of filtering are as follows: the micro algae culturing liquid of harvest is introduced into liquid storage facility；Then make micro algae culturing liquid in membrane filter system
It is recycled between liquid storage facility, controls the pressure difference of filter membrane two sides, made the partially liq in micro algae culturing liquid through filter membrane, filtering
Film two sides respectively obtain the algae solution of permeate and concentration.
Spray drying is implemented by YC-015 experiment type spray drier, condition are as follows: and 200 DEG C of inlet air temperature, leaving air temp
80 DEG C, sample introduction speed is 80mL/h.
The ingredient of BG11 culture medium is as follows:
Microelement A5 contains following ingredient:
Embodiment 1
Chlorella algae is purchased from Inst. of Hydrobiology, Chinese Academy of Sciences, BG11 culture medium culture.Algae is in the Portugal containing 5g/L
2d is induced in the BG11 culture medium of grape sugar, then access sterile in the illumination fermentation tank of the culture medium of BG11 containing 3L of 5L and is supported
It grows, algae solution OD after inoculation680Control is 1 or so, cultivating condition are as follows: 28 DEG C of temperature, ventilatory capacity about 0.9vvm, speed of agitator are about
280rpm, intensity of illumination 6000lux, light dark period 12h:12h are added antibiotic and control bacterial growth (kanamycins 50mg/L
With chloramphenicol 10mg/L), and be added 0.1 volume % defoaming agent (silicone emulsion).In breeding process, to cell density (with
Absorbance OD at 680nm680For index) it is monitored, nutritive salt is added according to the growth conditions of microalgae, to control grape
Sugared concentration is 5-10g/L, and the nitrogen source added is HNO3, HNO3Additional amount makes algae solution pH control between 7.5-9.After cultivating 3d
Taking out 90 volume % algae solutions, (the microalgae content in terms of dry weight enters membrane filter unit for 22.5g/L).
The inlet pressure 0.1MPa of membrane filter unit, outlet pressure 0.05MPa, the molecular cut off 30kDa of film, after filtering
Obtaining permeate and algae solution concentrate, (the microalgae content in terms of dry weight is 97.5g/L), permeate direct reuse, and concentrate carries out
Spray drying obtains microalgae product.
Permeate is added directly into 5L fermentor, using the remaining 10 volume % algae solution of previous sample as algae, Replacement Battalion
Continue to cultivate after supporting salt, antibiotic and defoaming agent, wherein nitrogen source is HNO3, cultivating condition is same as above, according to microalgae in breeding process
Growing state extra-nutrition salt and HNO3, after cultivating 3d, take out 90 volume % algae solutions and enter membrane filter unit, repeat above-mentioned step
Suddenly.
Chlorella growth curve is as shown in Figure 1.
Comparative example 1
Chlorella cultivating condition is with embodiment 1, except that algae solution is in fermentor after taking out, aseptically
Centrifugation, 4500r/min are centrifuged 15min, and then supernatant, which is refunded in fermentor, continues both culturing microalgae experiment.Chlorella growth curve
As shown in Figure 2.
Embodiment 2
Scenedesmus is purchased from Inst. of Hydrobiology, Chinese Academy of Sciences, BG11 culture medium culture.3L is added in 5L triangular flask
BG11 culture medium, is then inoculated with, OD after inoculation680Control is about 0.8, cultivating condition are as follows: 28 DEG C of temperature, ventilatory capacity 0.8-1vvm,
Light intensity 6000lux, light dark period 12h:12h, in breeding process, to cell density (with the absorbance OD at 680nm680To refer to
Mark) it is monitored, supplement HNO3With other nutritive salt, pH is controlled between 7-8, and the algae solution of 80 volume % is taken out after cultivation 7d
(the microalgae content in terms of dry weight is 1.2g/L) carries out film filtering.Membrane filter unit inlet pressure 0.1MPa, outlet pressure
0.05MPa, the molecular cut off 30kDa of film.The filtered concentrate of film (the microalgae content in terms of dry weight is 87g/L) is sprayed
Mist is dry, obtains microalgae product.It will transmit through liquid to refund in triangular flask and to sample remaining algae solution (20 volume %) as algae continuation
It cultivates, according to the growing state extra-nutrition salt and HNO of microalgae in breeding process3, repeat aforesaid operations.
Scenedesmus growth curve is as shown in Figure 3.
Comparative example 2
Scenedesmus cultivating condition is with embodiment 2, except that algae solution is in fermentor after taking out, aseptically from
The heart is centrifuged 15min in 4500r/min, and then supernatant, which is refunded in fermentor, continues both culturing microalgae experiment.The growth curve of scenedesmus
As shown in Figure 4.
Embodiment 3
The culture chlorella in the way of embodiment 1, unlike, take the algae solution (microalgae in terms of dry weight of 70 volume %
Content is 19.7g/L) enter membrane filter unit.Microalgae content of the filtered concentrate of film in terms of dry weight is 87.9g/L, bead
Algae growth curve is as shown in Figure 5.
Embodiment 4
Chlorella cultivating condition is with embodiment 1, except that adding KNO in microalga cultivation process3It is small as nitrogen source
As shown in Figure 1, carrying out film filtering after cultivating for the first time, microalgae content of the algae solution in terms of dry weight is ball algae growth curve
22.6g/L, microalgae content of the filtered concentrate of film in terms of dry weight is 107.4g/L.
Embodiment 5
Scenedesmus cultivating condition is with embodiment 2, except that adding KNO in microalga cultivation process3As nitrogen source, scenedesmus
Growth curve is as shown in figure 3, carrying out microalgae content of the algae solution of film filtering in terms of dry weight is 1.3g/L, the filtered concentrate of film
Microalgae content in terms of dry weight is 94.8g/L.
Embodiment 6
Chlorella cultivating condition is with embodiment 1, except that the molecular cut off of film is 40kDa, chlorella growth is bent
Line is as shown in Figure 2.Film filtering is carried out after cultivating for the first time, microalgae content of the algae solution in terms of dry weight is 19.4g/L, film filtering
Microalgae content of the concentrate afterwards in terms of dry weight is 100.8g/L.
Embodiment 7
Scenedesmus cultivating condition is with embodiment 2, except that the molecular cut off of film is 40kDa, scenedesmus growth curve is such as
Shown in Fig. 4, film filtering is carried out after cultivating for the first time, microalgae content of the algae solution in terms of dry weight is 1.4g/L, and film is filtered
Microalgae content of the concentrate in terms of dry weight is 98.3g/L.
Embodiment 8
Chlorella algae is purchased from Inst. of Hydrobiology, Chinese Academy of Sciences, BG11 culture medium culture.Algae is in the Portugal containing 5g/L
2d is induced in the BG11 culture medium of grape sugar, then access sterile in the illumination fermentation tank of the culture medium of BG11 containing 3L of 5L and is supported
It grows, algae solution OD after inoculation680Control is 1 or so, cultivating condition are as follows: 28 DEG C of temperature, ventilatory capacity about 0.9vvm, speed of agitator are about
280rpm, intensity of illumination 6000lux, light dark period 12h:12h are added antibiotic and control bacterial growth (kanamycins 50mg/L
With chloramphenicol 10mg/L), and be added 0.1 volume % defoaming agent (silicone emulsion).In breeding process, to cell density (with
Absorbance OD at 680nm680For index) it is monitored, nutritive salt is added according to the growth conditions of microalgae, to control grape
Sugared concentration is 5-10g/L, and the nitrogen source added is HNO3, HNO3Additional amount makes algae solution pH control between 7.5-9.After cultivating 3d
Taking out 90 volume % algae solutions, (the microalgae content in terms of dry weight enters membrane filter unit for 22.5g/L).
The inlet pressure 0.1MPa of membrane filter unit, outlet pressure 0.05MPa, the molecular cut off 150kDa of film, filtering
Obtaining permeate and algae solution concentrate afterwards, (the microalgae content in terms of dry weight is 125.4g/L), and concentrate is spray-dried, and is obtained
Obtain microalgae product.
Embodiment 9
Scenedesmus is purchased from Inst. of Hydrobiology, Chinese Academy of Sciences, BG11 culture medium culture.3L is added in 5L triangular flask
BG11 culture medium, is then inoculated with, OD after inoculation680Control is about 0.8, cultivating condition are as follows: 28 DEG C of temperature, ventilatory capacity 0.8-1vvm,
Light intensity 6000lux, light dark period 12h:12h, in breeding process, to cell density (with the absorbance OD at 680nm680To refer to
Mark) it is monitored, supplement HNO3With other nutritive salt, pH is controlled between 7-8, and the algae solution of 80 volume % is taken out after cultivation 7d
(the microalgae content in terms of dry weight is 1.2g/L) carries out film filtering.Membrane filter unit inlet pressure 0.1MPa, outlet pressure
0.05MPa, the molecular cut off 100kDa of film.The filtered concentrate of film (the microalgae content in terms of dry weight is 104.8g/L) into
Row spray drying, obtains microalgae product.
The energy consumption of cultural method of the invention and conventional centrifugal method is counted it can be concluded that, method of the invention
Energy consumption be only centrifugal method half or lower, therefore method of the invention has the advantage that low energy consumption.
By result above as can be seen that the present invention can effective microalgae, stability is strong, largely saves
Energy consumption, reduces the yield of waste water, realizes the green harvesting of microalgae.
The preferred embodiment of the present invention has been described above in detail, and still, the present invention is not limited thereto.In skill of the invention
In art conception range, can with various simple variants of the technical solution of the present invention are made, including each technical characteristic with it is any its
Its suitable method is combined, and it should also be regarded as the disclosure of the present invention for these simple variants and combination, is belonged to
Protection scope of the present invention.
Claims (10)
1. a kind of method of microalgae, which is characterized in that this method includes the microdisk electrode using membrane filter system to harvest
The step of liquid is concentrated: in the step, the micro algae culturing liquid of harvest is recycled in the circuit for being equipped with membrane filter system, microalgae
When culture solution flows through the filter membrane in membrane filter system, the partially liq in micro algae culturing liquid penetrates filter membrane, distinguishes in filter membrane two sides
Obtain the algae solution of permeate and concentration.
2. according to the method described in claim 1, wherein, at the end of the concentration step, in the algae solution of concentration in terms of dry weight
Microalgae content is 80-150g/L.
3. according to the method described in claim 1, wherein, the method also includes the micro algae culturing liquids to concentration to be done by spraying
It is dry, to obtain the step of doing microalgae.
4. according to the method described in claim 1, wherein, molecular cut off≤300kDa of the filter membrane, preferably≤30kDa.
5. method described in any one of -4 according to claim 1, wherein the mode of the concentration are as follows: by the microalgae of harvest
Culture solution recycles between membrane filter system and liquid storage facility, and the pressure difference of control filter membrane two sides is 0.02-0.05MPa, makes micro-
Partially liq in algae culturing liquid penetrates filter membrane, respectively obtains the algae solution of permeate and concentration in filter membrane two sides.
6. a kind of method for obtaining microalgae biomass includes the steps that cultivating microalgae, microalgae and extracts microalgae biomass,
It is characterized in that, using method microalgae described in any one of claim 1-5.
7. according to the method described in claim 6, wherein, the method also includes by the NO in flue gasxIt is converted into NO3 -And/or
NO2 -, and using it as incubation step in cultivate microalgae nitrogen source.
8. according to the method described in claim 6, wherein, it is 15-40 DEG C that the condition for cultivating microalgae, which includes: temperature, intensity of illumination
For 1000-200000lux, pH value 6.5-10, ventilatory capacity 0.5-1.5vvm.
9. according to method described in claim 6,7 or 8, wherein the microalgae is can be with NO3 -And/or NO2 -As nitrogen source
Algae, preferably cyanobacteria or green alga, more preferably chlorella, single needle algae, scenedesmus or spirulina.
10. according to the method described in claim 6, wherein, in incubation step, at the end of culture, leaving part micro algae culturing liquid
The microdisk electrode of next round is carried out as algae, remaining micro algae culturing liquid carries out harvesting step；Preferably, 10-20 body is left
The micro algae culturing liquid of product % carries out the microdisk electrode of next round as algae, remaining micro algae culturing liquid carries out harvesting step.
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| CN110066736A (en) * | 2018-01-23 | 2019-07-30 | 中国石油化工股份有限公司 | The method and system of Cyclic culture microalgae |
| CN113735265A (en) * | 2020-05-29 | 2021-12-03 | 中国石油化工股份有限公司 | Method for treating phosphorus-containing wastewater |
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| CN105861370A (en) * | 2016-04-25 | 2016-08-17 | 江南大学 | High-density culture and pre-harvesting method of microalgae |
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| CN110066736A (en) * | 2018-01-23 | 2019-07-30 | 中国石油化工股份有限公司 | The method and system of Cyclic culture microalgae |
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