CN104830665A - Method and special device for cultivating spirulina - Google Patents

Method and special device for cultivating spirulina Download PDF

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Publication number
CN104830665A
CN104830665A CN201510279084.1A CN201510279084A CN104830665A CN 104830665 A CN104830665 A CN 104830665A CN 201510279084 A CN201510279084 A CN 201510279084A CN 104830665 A CN104830665 A CN 104830665A
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spirulina
cultivation
liquid
mixing kettle
gas
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郑祖凤
郑训艺
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Jiangxi Three Da Xin Great Ze Biotechnology Co Ltd
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Jiangxi Three Da Xin Great Ze Biotechnology Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cultivation Of Seaweed (AREA)
  • Hydroponics (AREA)

Abstract

The invention discloses a method and a special device for cultivating spirulina. The special device comprises a cultivation pond, a separation device, a liquid return pond and a mixing kettle. The mixing kettle is connected with a CO<2> absorption tower, and the liquid return pond is provide with a circulating pump and is connected with the cultivation pond by the circulating pump. The method for cultivating the spirulina includes steps of (1), filtering concentrated lake water by the aid of biological nets, filling the cultivation pond with the lake water, adjusting the concentration of the lake water until the concentration of the lake water ranges from 1 baume degree to 6 baume degrees, adding cultivation liquid suitable for growth of the spirulina into the lake water, adding CO<2> gas into return water in the special device for cultivating the spirulina in cultivation procedures, adding circulating cultivation liquid with the CO<2> gas into the cultivation liquid in the cultivation liquid and controlling a PH (potential of hydrogen) value of liquid so that the PH value of the liquid ranges from 9.5 to 10.5; (2), domesticating the spirulina; (3), cultivating the spirulina; (4), harvesting the spirulina; (5), recycling filter liquid, treating the filter liquid by the aid of backflow pipes and reusing the filter liquid. The circulating cultivation liquid is mixed with the CO<2> gas by the aid of the mixing kettle.

Description

The method of cultivation of spirulina and special purpose device
Technical field
The present invention relates to the Cultivating techniques field of spirulina, relate in particular to a kind of method of cultivation and special purpose device of spirulina.
Background technology
Spirulina belongs to Cyanophyta, Cyanophyceae, breaks and grow body order, Oscillariaceae, Spirullina, phycophyta, the photosynthetic autotrophs biology that a kind of cell physiological result is similar to bacterium, in blackish green, it is abundant, the most balanced natural nutrition subsidy food of the nutrition that finds up to now, it contains the daily necessary protein of the mankind, and the Amino Acid Composition forming protein is in admirable proportion, also some human body very unobtainable composition from other food, and its digestibility is up to 95%, very easily by human consumption and absorption.Current Spirulina Products has been applied to the mankind and has eaten field, and spirulina has good anti-virus ability, can effectively reduce blood sugar, cholesterol, is the medical health care products that a kind of value is higher.
Traditional spirulina breeding all adopts freshwater aquiculture or sea farming, and the spirulina of gained is called fresh water spirulina or seawater spirulina, and these two kinds of breeding way, all need the culturing pool of applicable growth of spirulina platensis.At present, the research that the technical study of spirulina relatively reduces costs, such as, publication number is the application for a patent for invention of CN1510123, is exactly that one utilizes Na 2cO 3-NaHCO 3the method of type saline and alkaline lake water concentrated solution pilot scale culture spirulina, its technical measures are exactly lake water concentrated solution 1 ~ 6 degree Beaume adopting the type, add nitrogenous source (as NaNO 31 ~ 2.5g/L, urea 00.3 ~ 0.1g/L), phosphoric acid diamines 0.3 ~ 0.7g/L, Na-EDTA0.03 ~ 0.1g/L, be mixed with the nutrient solution being suitable for growth of spirulina platensis, in breeding process, controls the pH value 8.5 ~ 11 of nutrient solution.Yield of spirulina just can reach 6 ~ 8g/m 2d, thus can reduce costs.
But the cost of above-mentioned cultural method is still very high, in breeding process, Na 2cO 3-NaHCO 3the function of adjust ph is bad, and effect is bad.
Summary of the invention
For the defect of above-mentioned prior art, the invention provides a kind of method of cultivation and special purpose device of spirulina, present method technique is simple, and cost is low, can promote to improve growing of spirulina, improves spirulina quality, improves output.
For achieving the above object, the technical solution used in the present invention is:
Spirulina cultivate a special purpose device, comprise the cultivation pool for cultivating spirulina, for spirulina be separated tripping device and liquid return tank, described cultivation pool is connected with Na 2cO 3or NaHCO 3the entrance of material, with Na 2cO 3or NaHCO 3charging pump be connected, the filtrate outlet of described cultivation pool is connected with the entrance of described tripping device, described tripping device to be connected with described liquid return tank by a return line and to be connected, and the return line increase between described tripping device and liquid return tank is provided with mixing kettle, and described mixing kettle is externally connected with CO 2gas add pipe, with CO 2absorption tower is connected, and described liquid return tank is provided with communicating pipe, and described communicating pipe is provided with recycle pump, and the exit end of described communicating pipe is connected with cultivation pool.With recycle pump drive nutrient solution circulation, carbonic acid gas adds from mixing kettle, mixes with nutrient solution, return line dissolved under pressure in the medium, then send into cultivation pool by recycle pump.
The present invention also provides and utilizes above-mentioned special purpose device to carry out the method for cultivation of spirulina cultivation, and the step of this method of cultivation is:
(1) lake water of natural evaporation concentration 200 ~ 600 object biological nets are filtered, then the condensed water of filtering is injected cultivation pool, its concentration is made to adjust to 1 ~ 6 degree Beaume, add the nutrient solution being suitable for growth of spirulina platensis, in breeding process, the liquid water that returns cultivated by spirulina in special purpose device adds CO 2gas, what mix through mixing kettle contains CO 2the Cyclic culture liquid of gas is added in the nutrient solution of cultivation pool, controls the pH value of solution between 9.5 ~ 10.5;
(2) domestication of spirulina: algae kind is inoculated in fresh water or seawater and carries out domestication cultivation;
(3) cultivation of spirulina: the spirulina of logarithmic phase in step (2) is inoculated in the nutrient solution of step (1) and cultivates;
(4) the gathering of spirulina: step (3) is cultivated gained spirulina and filters, cleaning, regathers spirulina, then carry out drying to spirulina;
(5) filtrate is reclaimed, and again utilizes after return line process.
Compared with prior art, the beneficial effect acquired by the present invention is:
Method of cultivation of the present invention and Patent equipment, by CO 2carbon source adds the cultivation pool cultivating spirulina to as a supplement, increases CO2 meltage, transform, will greatly reduce NaHCO in conventional medium to original culture process with mixing kettle 3addition, reduce costs, increase CO2 utilization ratio, reduce greenhouse gas emission; Carbonic acid gas is than NaHCO3 and Na 2cO 3have better pH value regulatory function, spirulina optimal pH=9.5 ~ 10.5, in culturing process, medium pH can be elevated to 11 ~ 12 gradually, unfavorable algal grown, and after CO2, pH can fall back to rapidly within the suitableeest scope, favourable algal grown.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Reference numeral: 1-cultivation pool; 2-tripping device; 3-liquid return tank; 4-charging pump; 5-mixing kettle; 6-absorption tower; 7-recycle pump.
Embodiment
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below, and the technical scheme in the embodiment of the present invention is clearly and completely described, apparently, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art are not making the every other embodiment obtained under creative work prerequisite, and any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Embodiment one
As shown in Figure 1, the special purpose device that spirulina of the present invention is cultivated, the tripping device 2 comprise the cultivation pool 1 for cultivating spirulina, being separated for spirulina and liquid return tank 3, described cultivation pool 1 is connected with Na 2cO 3or NaHCO 3the entrance of material, with Na 2cO 3or NaHCO 3charging pump 4 be connected, the filtrate outlet of described cultivation pool 1 is connected with the entrance of described tripping device 2, described tripping device 2 to be connected with described liquid return tank 3 by a return line and to be connected, return line increase between described tripping device 2 and liquid return tank 3 is provided with mixing kettle 5, and described mixing kettle 5 is externally connected with CO 2gas add pipe, with CO 2absorption tower 6 is connected, and described liquid return tank 3 is provided with communicating pipe, and described communicating pipe is provided with recycle pump 7, and the exit end of described communicating pipe is connected with cultivation pool.With recycle pump drive nutrient solution circulation, carbonic acid gas adds from mixing kettle, mixes with nutrient solution, return line dissolved under pressure in the medium, then send into cultivation pool by recycle pump.
Its processing step is:
(1) lake water of natural evaporation concentration 200 object biological nets are filtered, then the condensed water of filtering is injected cultivation pool, make its concentration adjust to 1 degree Beaume, add the nutrient solution being suitable for growth of spirulina platensis, in breeding process, the liquid water that returns cultivated by spirulina in special purpose device adds CO 2gas, what mix through mixing kettle contains CO 2the Cyclic culture liquid of gas is added in the nutrient solution of cultivation pool, controls the pH value of solution 9.5;
(2) domestication of spirulina: algae kind is inoculated in fresh water or seawater and carries out domestication cultivation;
(3) cultivation of spirulina: the spirulina of logarithmic phase in step (2) is inoculated in the nutrient solution of step (1) and cultivates;
(4) the gathering of spirulina: step (3) is cultivated gained spirulina and filters, cleaning, regathers spirulina, then carry out drying to spirulina;
(5) filtrate is reclaimed, and again utilizes after return line process.
Embodiment two
Its processing step is:
(1) lake water of natural evaporation concentration 600 object biological nets are filtered, then the condensed water of filtering is injected cultivation pool, make its concentration adjust to 6 degree Beaume, add the nutrient solution being suitable for growth of spirulina platensis, in breeding process, the liquid water that returns cultivated by spirulina in special purpose device adds CO 2gas, what mix through mixing kettle contains CO 2the Cyclic culture liquid of gas is added in the nutrient solution of cultivation pool, controls the pH value of solution 10.5;
(2) domestication of spirulina: algae kind is inoculated in fresh water or seawater and carries out domestication cultivation;
(3) cultivation of spirulina: the spirulina of logarithmic phase in step (2) is inoculated in the nutrient solution of step (1) and cultivates;
(4) the gathering of spirulina: step (3) is cultivated gained spirulina and filters, cleaning, regathers spirulina, then carry out drying to spirulina;
(5) filtrate is reclaimed, and again utilizes after return line process.
Embodiment three
Its processing step is:
(1) lake water of natural evaporation concentration 400 object biological nets are filtered, then the condensed water of filtering is injected cultivation pool, make its concentration adjust to 3 degree Beaume, add the nutrient solution being suitable for growth of spirulina platensis, in breeding process, the liquid water that returns cultivated by spirulina in special purpose device adds CO 2gas, what mix through mixing kettle contains CO 2the Cyclic culture liquid of gas is added in the nutrient solution of cultivation pool, controls the pH value of solution 10;
(2) domestication of spirulina: algae kind is inoculated in fresh water or seawater and carries out domestication cultivation;
(3) cultivation of spirulina: the spirulina of logarithmic phase in step (2) is inoculated in the nutrient solution of step (1) and cultivates;
(4) the gathering of spirulina: step (3) is cultivated gained spirulina and filters, cleaning, regathers spirulina, then carry out drying to spirulina;
(5) filtrate is reclaimed, and again utilizes after return line process.

Claims (2)

1. spirulina cultivate a special purpose device, it is characterized in that: comprise the cultivation pool for cultivating spirulina, for spirulina be separated tripping device and liquid return tank, described cultivation pool is connected with Na 2cO 3or NaHCO 3the entrance of material, with Na 2cO 3or NaHCO 3charging pump be connected, the filtrate outlet of described cultivation pool is connected with the entrance of described tripping device, described tripping device to be connected with described liquid return tank by a return line and to be connected, and the return line increase between described tripping device and liquid return tank is provided with mixing kettle, and described mixing kettle is externally connected with CO 2gas add pipe, with CO 2absorption tower is connected, and described liquid return tank is provided with communicating pipe, and described communicating pipe is provided with recycle pump, and the exit end of described communicating pipe is connected with cultivation pool.With recycle pump drive nutrient solution circulation, carbonic acid gas adds from mixing kettle, mixes with nutrient solution, return line dissolved under pressure in the medium, then send into cultivation pool by recycle pump.
2. the special purpose device using the spirulina described in claim 1 to cultivate carries out a method for spirulina cultivation, it is characterized in that: the step of this method of cultivation is:
(1) lake water of natural evaporation concentration 200 ~ 600 object biological nets are filtered, then the condensed water of filtering is injected cultivation pool, its concentration is made to adjust to 1 ~ 6 degree Beaume, add the nutrient solution being suitable for growth of spirulina platensis, in breeding process, the liquid water that returns cultivated by spirulina in special purpose device adds CO 2gas, what mix through mixing kettle contains CO 2the Cyclic culture liquid of gas is added in the nutrient solution of cultivation pool, controls the pH value of solution between 9.5 ~ 10.5;
(2) domestication of spirulina: algae kind is inoculated in fresh water or seawater and carries out domestication cultivation;
(3) cultivation of spirulina: the spirulina of logarithmic phase in step (2) is inoculated in the nutrient solution of step (1) and cultivates;
(4) the gathering of spirulina: step (3) is cultivated gained spirulina and filters, cleaning, regathers spirulina, then carry out drying to spirulina;
(5) filtrate is reclaimed, and again utilizes after return line process.
CN201510279084.1A 2015-05-28 2015-05-28 Method and special device for cultivating spirulina Pending CN104830665A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106718815A (en) * 2016-12-15 2017-05-31 志成信（天津）科技有限公司 Experimental facility of mechatronics with pharmaceutical intervention
CN108410704A (en) * 2018-03-30 2018-08-17 福州大学 A kind of locellus bioreactor and its method
CN110106084A (en) * 2019-05-17 2019-08-09 西南交通大学 Cell culture apparatus and the method for making culture medium hydrogen-rich

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1510123A (en) * 2002-12-23 2004-07-07 尹伟伦 Culture of spirulina by concentrated Na2 Co3-NaHCO3 saline-alakli lake water
CN102533560A (en) * 2012-01-13 2012-07-04 北京林业大学 Method for using CO2-containing waste gas in microalgae culture
CN102533527A (en) * 2011-12-18 2012-07-04 鄂托克旗隆诚化工有限责任公司 Process and system for preparing spirulina culture solution from low-concentration alkaline lake brine
CN202322809U (en) * 2011-11-11 2012-07-11 张宏彬 System for producing spirulina by using CO2 recovered from waste flue gas
DE102013001689A1 (en) * 2013-01-31 2014-07-31 Waldemar E. Reule Generating biomethane from biogas or digester gas and hydrogen, comprises e.g. feeding biogas from biogas plant or digestion tower, mixed with hydrogen to biomethanation reactor, and reacting carbon dioxide with hydrogen by methane bacteria

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1510123A (en) * 2002-12-23 2004-07-07 尹伟伦 Culture of spirulina by concentrated Na2 Co3-NaHCO3 saline-alakli lake water
CN202322809U (en) * 2011-11-11 2012-07-11 张宏彬 System for producing spirulina by using CO2 recovered from waste flue gas
CN102533527A (en) * 2011-12-18 2012-07-04 鄂托克旗隆诚化工有限责任公司 Process and system for preparing spirulina culture solution from low-concentration alkaline lake brine
CN102533560A (en) * 2012-01-13 2012-07-04 北京林业大学 Method for using CO2-containing waste gas in microalgae culture
DE102013001689A1 (en) * 2013-01-31 2014-07-31 Waldemar E. Reule Generating biomethane from biogas or digester gas and hydrogen, comprises e.g. feeding biogas from biogas plant or digestion tower, mixed with hydrogen to biomethanation reactor, and reacting carbon dioxide with hydrogen by methane bacteria

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
白坤等: "《玉米淀粉工程技术》", 31 January 2012 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106718815A (en) * 2016-12-15 2017-05-31 志成信（天津）科技有限公司 Experimental facility of mechatronics with pharmaceutical intervention
CN108410704A (en) * 2018-03-30 2018-08-17 福州大学 A kind of locellus bioreactor and its method
CN110106084A (en) * 2019-05-17 2019-08-09 西南交通大学 Cell culture apparatus and the method for making culture medium hydrogen-rich

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Application publication date: 20150812