CN105505779A - Resource utilization method of livestock excrement water-soluble manure production wastewater for culturing dunaliella - Google Patents
Resource utilization method of livestock excrement water-soluble manure production wastewater for culturing dunaliella Download PDFInfo
- Publication number
- CN105505779A CN105505779A CN201510886188.9A CN201510886188A CN105505779A CN 105505779 A CN105505779 A CN 105505779A CN 201510886188 A CN201510886188 A CN 201510886188A CN 105505779 A CN105505779 A CN 105505779A
- Authority
- CN
- China
- Prior art keywords
- water
- livestock
- resource utilization
- feces
- utilization method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/44—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis
- C02F1/444—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis by ultrafiltration or microfiltration
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2301/00—General aspects of water treatment
- C02F2301/08—Multistage treatments, e.g. repetition of the same process step under different conditions
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Sustainable Development (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Hydrology & Water Resources (AREA)
- Botany (AREA)
- Biodiversity & Conservation Biology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The invention belongs to the field of wastewater treatment, and provides a resource utilization method of livestock excrement water-soluble manure production wastewater for culturing dunaliella. The method comprises the following steps: 1) diluting livestock excrement water-soluble manure production wastewater with water, 2) adding nutrient elements like P, Ca, Fe and Mg into the diluted livestock excrement water-soluble manure production wastewater, 3) using one or more of a NaCl solution, seawater and brine to adjust the salinity value of the wastewater to be 150-200, and controlling the pH value to be 8.0-9.0, (4) vaccinating dunaliella in the logarithmic growth phase for culture, and 5) continuously culturing for 8-12 d so as to obtain dunaliella after dunaliella grows to be in the platform phase. According to the provided method, treatment of the livestock excrement water-soluble manure production wastewater and recycle of the nutrient elements are combined, pollutants in the wastewater can be desorbed, biomass raw materials can be produced, and multiple efficacies of environmental protection, cost saving, resource recycle and nutrient element cyclic utilization are played.
Description
Technical field
The invention belongs to field of waste water treatment, be specifically related to a kind of method of wastewater treatment utilizing microorganism.
Background technology
Livestock/poultry biogas slurry is the debris of feces of farm after anaerobically fermenting, belongs to the organic waste water of high density, wherein containing soluble substances such as ammonium salt, sylvite, phosphoric acid salt.The nutritive elements such as N, P, K in the liquid of natural pond.Natural pond liquid can be discharged in soil as organic fertilizer to create more economic worth.The natural pond liquid of output usually passes through supporting soil and dissolves abroad, but due to domestic too much mistake be that concentrated natural pond liquid output makes biogas production business not have enough soils to dissolve.If a large amount of natural pond liquid is without disposal, be not only the waste of resource, also can cause huge pressure to the environment such as soil, water body.
Now, film filtering system process natural pond liquid is utilized to obtain a lot of concern and application.Natural pond liquid concentrates through membrane filtration, forms concentrated solution and clear liquid.Wherein concentrated solution can be used as Water soluble fertilizer after treatment, and a large amount of clear liquid carries out innoxious, recycling by cultivating micro-algae wherein.
Micro-algae is subject to extensive concern with its high photosynthetic efficiency, faster reproduction speed as photoautotrophy type biology all the time.In the process of growth of micro-algae, rely on the photosynthetic efficiency that it is higher, absorb the CO in air
2, it can be used as the carbon source of synthesis own cells material, reach CO in fixed air
2object.Have algae slightly that the organic carbon source in water can also be utilized to carry out facultative growth, what simultaneously stability water body had contains N, P-compound, thus achieves the organic degraded in water body.
Micro-algae, as the photosynthetic growing plants of battalion, can produce the meta-bolites much with high added value, as pigment, unsaturated fatty acids, protein, VITAMIN, polysaccharide, somatomedin etc. in its photosynthesis.These products make micro-algae all have very high utility value in a lot.β-carotene (C
40h
56) be one of carotenoid, being safran fat-soluble cpds, be the most ubiquity of occurring in nature is also the most stable natural pigment.β-carotene is present in a lot of natural food, and as in pawpaw, Radix Dauci Sativae etc., and some kinds of Dunaliella salina can at the higher content beta-carotene of oneself accumulation, and some even can reach 14% of own cells dry weight.
Summary of the invention
For the weak point of this area, the object of the invention is to propose the resource utilization method that a kind of feces of livestock and poultry Water soluble fertilizer factory effluent cultivates Dunaliella salina.
This processing method is divided into three parts, and feces of livestock and poultry Water soluble fertilizer factory effluent is diluted in Dunaliella salina growth optimum range by first part; Second section is the concentration and the proportioning that regulate feces of livestock and poultry Water soluble fertilizer factory effluent Middle nutrition element, and regulates salt angle value, pH value to reach the envrionment conditions of applicable Dunaliella salina growth; Part III in pretreated feces of livestock and poultry Water soluble fertilizer factory effluent, accesses Dunaliella salina and passes into CO
2gas cultivates results Dunaliella salina.By the operation of above three parts, realize the recovery to ammonia nitrogen in waste water, and obtain high value Dunaliella salina, and for the production of carotene, effectively achieve waste water reclaiming and reduction of greenhouse gas discharge.
Realize the object of the invention technical scheme to be specially:
Feces of livestock and poultry Water soluble fertilizer factory effluent cultivates a resource utilization method for Dunaliella salina, comprises step:
1) by feces of livestock and poultry Water soluble fertilizer factory effluent dilute with water, extension rate is 20 ~ 35 times;
2) in the feces of livestock and poultry Water soluble fertilizer factory effluent after dilution, add nutritive element P, Ca, Fe, Mg, corresponding nutritive salt addition is NaH
2pO
42H
2o15.0 ~ 18.0mg/L, CaCl
22H
2o40 ~ 50mg/L, MgSO
47H
2o1000 ~ 1300mg/L, ironic citrate 4 ~ 6mg/L;
3) use salt angle value to 150 ~ 200 (usual salinity does not have unit, and known is ‰) of one or more adjustment waste water in NaCl solution, seawater, bittern, and control ph is 8.0 ~ 9.0;
4) to step 1) inoculation is in logarithmic phase in waste water after dilution Dunaliella salina (Dunaliellasp.) cultivates, and inoculum size is 0.2g dry weight/L to 0.3g dry weight/L;
5) cultured continuously 8 ~ 12 days, after making the growth of micro-algae arrive plateau, by microalgae harvesting, and producing for β-carotene.
The Dunaliella salina (Dunaliellasp.) that the present invention uses, for Dunaliella belongs to, algae strain is numbered: FACHB-558, purchased from aquatic institute of the Chinese Academy of Sciences (address: hydrobiont institute of Chinese Academy of Sciences algae kind storehouse, No. 7, South Road, East Lake, Luo Jia Shan, Wuhan).
Wherein, described feces of livestock and poultry is chicken manure and/or pig manure, and in described feces of livestock and poultry Water soluble fertilizer factory effluent, COD concentration is 800 ~ 900mg/L, TN is 2800 ~ 3100mg/L, NH
3-N concentration is 2000 ~ 2500mg/L, TP concentration, 6.0 ~ 10.0mg/L.
Through step 1) dilution, the NH of waste water
3-N concentration can reach 70 ~ 120mg/L, is Dunaliella salina growth optimum range.
Step 2) Middle nutrition salt NaH
2pO
42H
2o, CaCl
22H
2o, MgSO
47H
2o, ironic citrate should add in order, dissolves completely add next medicine successively at last medicine.Or be mixed with mother liquor and add.
Preferably, described step 4) in, cultivation illuminance is 1800 ~ 2500lux, and light dark period is than being 12:10 ~ 14.
Wherein, described step 4) in, pass into CO in the photoperiod of microdisk electrode
2volume ratio is the CO of 4 ~ 10%
2with air gas mixture.
Wherein, described step 3) in, described seawater and bittern are through sterilization, and described sterilization is by liquid sterilizing 15 ~ 30min at 120 ~ 130 DEG C.
Wherein, described step 3) middle by 0.2 ~ 2mol/L hydrochloric acid and/or 0.2 ~ 2mol/LNaOH solution adjust ph.
More preferably, described step 3) in, with the seawater after NaCl solution or sterilization, a kind of salt angle value to 165 ~ 175 regulating waste water in bittern, use hydrochloric acid and/or NaO solution adjust ph to 8.5 afterwards.
Wherein, described step 5) in, after making the growth of micro-algae arrive plateau, with inner pressed vacuum tunica fibrosa by microalgae harvesting, the aperture of hollow-fibre membrane is 0.22 μm.
Further, the present invention also proposes the equipment that described resource utilization method uses.
Equipment for described method comprises bioreactor, ultra filtration unit, micro-algae seed culture device, air admission unit;
Described bioreactor is provided with feces of livestock and poultry Water soluble fertilizer factory effluent water-in, and micro-algae seed culture device connects described bioreactor by pipeline
Described air admission unit comprises CO
2admission passage and air compressor, described CO
2admission passage and air compressor are connected with mixed air vessel, and described mixed air vessel is connected with aeration head by pipeline, and described aeration head is arranged on bottom bioreactor;
The algae of described bioreactor is connected described ultra filtration unit with waste water mixture export.
Wherein, described ultra filtration unit comprises inner pressed hollow fiber film assembly, and the outlet of bioreactor algae liquid connects the water-in of described inner pressed hollow fiber film assembly, connecting pipeline is provided with tensimeter, surge pump, valve, spinner-type flowmeter; The concentrated solution outlet of described inner pressed hollow fiber film assembly is connected with micro-algae concentrated solution hold-up vessel.
Particularly, described method, come by following steps:
One, by water pump, in reactor body, pump into feces of livestock and poultry Water soluble fertilizer factory effluent by water intake, add distilled water;
Two, nutritive salt NaH is added respectively in order to waste water in reactor
2pO
42H
2o, CaCl
22H
2o, MgSO
47H
2o, ironic citrate.Note adding in order, dissolve completely at last medicine and add next medicine successively.Or be mixed with mother liquor and add.Illuminance and light dark period ratio are cultivated in setting;
Three, in the waste water handled well, add micro-algae seedling solution, until being assigned to microalgae dry weight is 0.2 ~ 0.3g/L, total amount of liquid is 2/3rds of reactor volume;
Four, regulate waste water ph to 8.5, open mixing air-channel system.Open CO
2pressure control valve and air compressor, regulate CO
2flow takes into account air intlet under meter, in reactor, pass into CO
2and air gas mixture, cultivate 8 ~ 12 days;
Five, ultrafiltration system is opened.Open microdisk electrode device water inlet valve, open surge pump, regulates liquid under meter, control flooding velocity and the pressure of ultra-filtration membrane; Clear liquid after ultrafiltration flows out through hollow fiber film assembly purified liquor outlet, and the micro-algae concentrated solution after ultrafiltration is flowed out by membrane module concentrated solution outlet, collects micro-algae concentrated solution with storage tank.
Beneficial effect of the present invention is:
The present invention propose recovery feces of livestock and poultry Water soluble fertilizer factory effluent Middle nutrition element and the method for output high added value Dunaliella salina; it is the method for process feces of livestock and poultry Water soluble fertilizer factory effluent, nutritive element recycling and biomass being produced wastewater treatment and the resource utilization combined; both removing of Pollutants in Wastewater can be realized; the production of biomass material can be accomplished again, serve the multi-efficiency that environment protection, cost-saving, Resource recovery and Nutrient Cycling utilize.
The output of high value biomass and sewage disposal combine by the present invention, create extra value to a certain extent, thus further save the Financial cost of wastewater treatment.After this process process, the Pollutant levels of feces of livestock and poultry Water soluble fertilizer factory effluent reduce more than 90%, dramatically reduce the subsequent disposal burden of this waste water.
Accompanying drawing explanation
Fig. 1 is feces of livestock and poultry Water soluble fertilizer factory effluent Middle nutrition element recycling equipment structure diagram of the present invention.
In figure, numbering and component names corresponding relation are:
Fig. 2 is embodiment 1 and comparative example biomass variation diagram in time.
Fig. 3 is embodiment 1 and comparative example β-carotene variation diagram in time.
Embodiment
Below in conjunction with drawings and Examples, the specific embodiment of the present invention is described in further detail.
If not otherwise specified, the means adopted in embodiment are the technique means of this area routine.
Embodiment 1
In the present embodiment, the chicken manure Water soluble fertilizer factory effluent of process, COD is 823.6mg/L, and its TN is 2979.3mg/L, NH
3-N concentration is 2166.3mg/L, TP concentration 9.6mg/L.
As Fig. 1, the equipment for chicken manure Water soluble fertilizer factory effluent recycling comprises bioreactor 6, ultra filtration unit, micro-algae seedling solution incubator 4, air admission unit;
The chicken manure Water soluble fertilizer factory effluent of process is stored in feces of livestock and poultry Water soluble fertilizer factory effluent hold tank 1, the bottom of bioreactor 6 is connected to by the first surge pump 3, feces of livestock and poultry Water soluble fertilizer factory effluent entrance 2 and pipeline, waterwater entrance distance reactor bottom about 10cm, micro-algae seed culture device 4 is triangular flask, connects bioreactor 6 by pipeline.
Described air admission unit comprises CO
2admission passage and air compressor 11, CO
2admission passage and CO
2gas cylinder 12 connects, CO
2the other end of admission passage connects mixed air vessel 8, and air compressor also connects mixed air vessel 8, and mixed air vessel 8 is connected with aeration head 7 by pipeline, and this aeration head 7 is arranged on bottom bioreactor 6.Bioreactor 6 top is provided with escape pipe 5.The algae of bioreactor 6 is connected ultra filtration unit with waste water mixture export by valve 13.
Ultra filtration unit comprises inner pressed hollow fiber film assembly 17, and the aperture of hollow-fibre membrane is 0.22 μm; The outlet of bioreactor algae liquid connects the water-in of this inner pressed hollow fiber film assembly 17, connecting pipeline is provided with algae and waste water mixture export valve 13, second surge pump 14, spinner-type flowmeter 15 and tensimeter 16; The concentrated solution outlet of inner pressed hollow fiber film assembly 17 is connected with micro-algae concentrated solution hold-up vessel 19.The filtrate effluent mouth 20 of inner pressed hollow fiber film assembly 17 arranges filtrate effluent valve 18.
Treatment step:
One, by water pump, in reactor body, pump into feces of livestock and poultry Water soluble fertilizer factory effluent 5L by water intake, add distilled water 145L, final NH
3-N content is 72.21mg/L.
Two, nutritive salt NaH is added respectively in order to waste water in reactor
2pO
42H
2o2.34g (15.6mg/L), CaCl
22H
2o6.6g (44mg/L), MgSO
47H
2o184.5g (1230mg/L), ironic citrate 0.75g (5mg/L).Arranging and cultivating illuminance is 2000lux, and the ratio L:D=12:12 of light dark period, each cycle is 12 hours respectively.
Three, by bittern sterilizing 15 ~ 30min at 120 ~ 130 DEG C, regulate the salt angle value to 170 of waste water with the bittern after sterilization, then use 1mol/L salt acid for adjusting pH value to 8.5.In the waste water handled well, add micro-algae seedling solution, until being assigned to microalgae dry weight is 0.25g/L, total amount of liquid is 2/3rds of reactor body volume.
Four, regulate waste water ph to 8.5, open mixing air-channel system.Open CO
2pressure control valve and air compressor, regulate CO
2flow takes into account air intlet under meter, within the photoperiod, pass into the CO that volume ratio is 10% in reactor
2mixed gas, cultivates 12 days;
Five, ultrafiltration system is opened.Open microdisk electrode device water inlet valve, open surge pump, regulates liquid under meter, control flooding velocity and the pressure of ultra-filtration membrane; Clear liquid after ultrafiltration flows out through hollow fiber film assembly purified liquor outlet, and the micro-algae concentrated solution after ultrafiltration is flowed out by membrane module concentrated solution outlet, with the micro-algae concentrated solution of other container collection.
Comparative example
As a comparison (CK), adopt the equipment of embodiment 1, wherein place the D.M. standard medium (D.mmedium) of distilled water preparation in light reactor in kind, other operational conditions are with embodiment 1.
Through growth in 12 days, filter with hollow fiber film assembly, the micro-algae concentrated solution got in storage tank was tested.Biomass and the ammonia nitrogen removal frank of embodiment 1 and comparative example the results are shown in Table 1.Biomass changes to compare in time sees Fig. 2.Fig. 3 is embodiment 1 and comparative example β-carotene variation diagram in time.
Table 1 biomass and ammonia nitrogen removal frank
Embodiment 2
In the present embodiment, the chicken manure Water soluble fertilizer factory effluent of process, COD is 869.4mg/L, and its TN is 2892.3mg/L, NH
3-N concentration is 2151.2mg/L, TP concentration 9.5mg/L.
Treatment step:
One, by water pump, in reactor body, pump into feces of livestock and poultry Water soluble fertilizer factory effluent 5L by water intake, add distilled water 135L, final NH
3-N content is 100.6mg/L.；
Two, nutritive salt is added in order respectively to NaH to waste water in reactor
2pO
42H
2o15mg/L, CaCl
22H
2o40mg/L, MgSO
47H
2o1250mg/L, ironic citrate 4mg/L.Arranging and cultivating illuminance is 2000lux, and the ratio L:D=12:12 of light dark period, each cycle is 12 hours respectively.
Three, regulate the salt angle value to 170 of waste water by NaCl solution, then use 1mol/L sodium hydroxide solution adjust ph to 8.5.In the waste water handled well, add micro-algae seedling solution, until being assigned to microalgae dry weight is 0.28g/L, total amount of liquid is 2/3rds volumes of reactor body.
Four, regulate waste water ph to 8.5, open mixing air-channel system.Open CO
2pressure control valve and air compressor, regulate CO
2flow takes into account air intlet under meter, within the photoperiod, pass into the CO that volume ratio is 5% in reactor
2mixed gas, cultivates 11 days;
Five, ultrafiltration system is opened.Open microdisk electrode device water inlet valve, open surge pump, regulates liquid under meter, control flooding velocity and the pressure of ultra-filtration membrane; Clear liquid after ultrafiltration flows out through hollow fiber film assembly purified liquor outlet, and the micro-algae concentrated solution after ultrafiltration is flowed out by membrane module concentrated solution outlet, with the micro-algae concentrated solution of other container collection.In the micro-algae concentrated solution collected, biomass is 960mg/L, and β-carotene is 13mg/L.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite not departing from the technology of the present invention principle; can also make some improvement and replacement, these improve and replace and also should be considered as protection scope of the present invention.
Claims (10)
1. feces of livestock and poultry Water soluble fertilizer factory effluent cultivates a resource utilization method for Dunaliella salina, it is characterized in that, comprises step:
1) by feces of livestock and poultry Water soluble fertilizer factory effluent dilute with water, extension rate is 20 ~ 35 times;
2) in the feces of livestock and poultry Water soluble fertilizer factory effluent after dilution, add nutritive element P, Ca, Fe, Mg, corresponding nutritive salt addition is NaH
2pO
42H
2o15.0 ~ 18.0mg/L, CaCl
22H
2o40 ~ 50mg/L, MgSO
47H
2o1000 ~ 1300mg/L, ironic citrate 4 ~ 6mg/L;
3) use salt angle value to 150 ~ 200 of one or more adjustment waste water in NaCl solution, seawater, bittern, and control ph is 8.0 ~ 9.0;
4) to step 1) inoculation is in logarithmic phase in waste water after dilution Dunaliella salina cultivates, and inoculum size is 0.2g dry weight/L to 0.3g dry weight/L;
5) cultured continuously 8 ~ 12 days, after making the growth of Dunaliella salina arrive plateau, gathers in the crops Dunaliella salina, and producing for β-carotene.
2. resource utilization method according to claim 1, is characterized in that, described feces of livestock and poultry is chicken manure and/or pig manure, and in described feces of livestock and poultry Water soluble fertilizer factory effluent, COD concentration is 800 ~ 900mg/L, TN is 2800 ~ 3100mg/L, NH
3-N concentration is 2000 ~ 2500mg/L, TP concentration, 6.0 ~ 10.0mg/L.
3. resource utilization method according to claim 1, is characterized in that, described step 4) in, cultivation illuminance is 1800 ~ 2500lux, and light dark period is than being 12:10 ~ 14.
4. resource utilization method according to claim 3, is characterized in that, described step 4) in, pass into CO in the photoperiod that Dunaliella salina is cultivated
2volume ratio is the CO of 4 ~ 10%
2with air gas mixture.
5. recycling of water resource Application way according to claim 1, is characterized in that, described step 3) in, described seawater and bittern are through sterilization, and described sterilization is by liquid sterilizing 15 ~ 30min at 120 ~ 130 DEG C.
6. recycling of water resource Application way according to claim 1, is characterized in that, described step 3) middle by 0.2 ~ 2mol/L hydrochloric acid and/or 0.2 ~ 2mol/LNaOH solution adjust ph.
7. according to the arbitrary described resource utilization method of claim 1 ~ 6, it is characterized in that, described step 3) in, with the seawater after NaCl solution or sterilization, a kind of salt angle value to 165 ~ 175 regulating waste water in bittern, use hydrochloric acid and/or NaOH solution adjust ph to 8.5 afterwards.
8. according to the arbitrary described sewage recycling Application way of claim 1 ~ 6, it is characterized in that, described step 5) in, after making the growth of Dunaliella salina arrive plateau, gathered in the crops by Dunaliella salina with inner pressed vacuum tunica fibrosa, the pore size filter of tunica fibrosa is 0.22 μm.
9., according to the arbitrary described resource utilization method of claim 1 ~ 6, it is characterized in that, the equipment for described method comprises bioreactor, ultra filtration unit, micro-algae seed culture device, air admission unit;
Described bioreactor is provided with feces of livestock and poultry Water soluble fertilizer factory effluent water-in, and micro-algae seed culture device connects described bioreactor by pipeline
Described air admission unit comprises CO
2admission passage and air compressor, described CO
2admission passage and air compressor are connected with mixed air vessel, and described mixed air vessel is connected with aeration head by pipeline, and described aeration head is arranged on bottom bioreactor;
The algae of described bioreactor is connected described ultra filtration unit with waste water mixture export.
10. according to the arbitrary described resource utilization method of Claims 1 to 4, it is characterized in that, described ultra filtration unit comprises inner pressed hollow fiber film assembly, the outlet of bioreactor algae liquid connects the water-in of described inner pressed hollow fiber film assembly, connecting pipeline is provided with tensimeter, surge pump, valve, spinner-type flowmeter; The concentrated solution outlet of described inner pressed hollow fiber film assembly is connected with micro-algae concentrated solution hold-up vessel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510886188.9A CN105505779A (en) | 2015-12-04 | 2015-12-04 | Resource utilization method of livestock excrement water-soluble manure production wastewater for culturing dunaliella |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510886188.9A CN105505779A (en) | 2015-12-04 | 2015-12-04 | Resource utilization method of livestock excrement water-soluble manure production wastewater for culturing dunaliella |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105505779A true CN105505779A (en) | 2016-04-20 |
Family
ID=55714081
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510886188.9A Pending CN105505779A (en) | 2015-12-04 | 2015-12-04 | Resource utilization method of livestock excrement water-soluble manure production wastewater for culturing dunaliella |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105505779A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106865779A (en) * | 2017-04-01 | 2017-06-20 | 江西理工大学 | A kind of ion type rareearth ammonia nitrogen waste water bioremediation |
| CN108085258A (en) * | 2018-01-03 | 2018-05-29 | 山东华维藻业有限公司 | A kind of utilize puies forward the method that bromine bittern cultivates Dunaliella salina in banked track pond |
| CN109467191A (en) * | 2018-12-10 | 2019-03-15 | 中国石油化工股份有限公司 | The method for handling high nitre waste water using microalgae |
| CN112028251A (en) * | 2020-09-03 | 2020-12-04 | 上饶师范学院 | Method for improving ammonia nitrogen removal effect of microalgae on livestock and poultry breeding wastewater |
| CN114394677A (en) * | 2022-01-18 | 2022-04-26 | 山东海之宝海洋科技有限公司 | Method for treating salt-containing wastewater |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102863115A (en) * | 2011-07-07 | 2013-01-09 | 江南大学 | Method for treating fermentation industry waste water and producing algae powder by using microalgae |
| CN104250058A (en) * | 2014-09-16 | 2014-12-31 | 中国农业大学 | Comprehensive treatment method of wastewater generated in production of water-soluble fertilizer from bird guano fermentation liquor |
-
2015
- 2015-12-04 CN CN201510886188.9A patent/CN105505779A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102863115A (en) * | 2011-07-07 | 2013-01-09 | 江南大学 | Method for treating fermentation industry waste water and producing algae powder by using microalgae |
| CN104250058A (en) * | 2014-09-16 | 2014-12-31 | 中国农业大学 | Comprehensive treatment method of wastewater generated in production of water-soluble fertilizer from bird guano fermentation liquor |
Non-Patent Citations (2)
| Title |
|---|
| 尚常花等: "培养基成分含量对巴夫杜氏藻生长的影响", 《新能源进展》 * |
| 马冬冬: "基于微藻的光-膜组合式生物反应器处理海水养殖业废水", 《中国优秀博硕士学位论文全文数据库（硕士）工程科技Ⅰ辑》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106865779A (en) * | 2017-04-01 | 2017-06-20 | 江西理工大学 | A kind of ion type rareearth ammonia nitrogen waste water bioremediation |
| CN108085258A (en) * | 2018-01-03 | 2018-05-29 | 山东华维藻业有限公司 | A kind of utilize puies forward the method that bromine bittern cultivates Dunaliella salina in banked track pond |
| CN109467191A (en) * | 2018-12-10 | 2019-03-15 | 中国石油化工股份有限公司 | The method for handling high nitre waste water using microalgae |
| CN112028251A (en) * | 2020-09-03 | 2020-12-04 | 上饶师范学院 | Method for improving ammonia nitrogen removal effect of microalgae on livestock and poultry breeding wastewater |
| CN114394677A (en) * | 2022-01-18 | 2022-04-26 | 山东海之宝海洋科技有限公司 | Method for treating salt-containing wastewater |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103087919B (en) | Method and device for collecting microalgae through continuous culture and in-situ self-flocculation | |
| CN105505779A (en) | Resource utilization method of livestock excrement water-soluble manure production wastewater for culturing dunaliella | |
| CN205398604U (en) | Recycle thermal power plant flue gas waste heat and carbon dioxide's device | |
| CN105541057B (en) | A kind of method of pig farm biogas slurry recycling | |
| CN104762331A (en) | Method and culture device for coupling biogas fermentation with microalgae culture | |
| CN205294969U (en) | System for handle water -soluble fertile waste water of animal manure with little algae | |
| CN105254367A (en) | Method used for preparing drip-irrigation special-purpose fertilizer from biogas slurry | |
| CN102676391B (en) | Method and device for producing microalgae by byproducts CO2 and NH3 and waste water in chemical plant | |
| CN101503264A (en) | Method for ectopically and ecologically repairing eutrophication water | |
| CN103103128A (en) | Method for high efficiency enrichment culture of microalgae | |
| CN108358692A (en) | It is a kind of to utilize liquid fertilizer of livestock and poultry feces and preparation method thereof and its application process | |
| CN108713489A (en) | A kind of fish plants the synthesis intercropping cyclic culture pattern and method of symbiosis | |
| CN104250058A (en) | Comprehensive treatment method of wastewater generated in production of water-soluble fertilizer from bird guano fermentation liquor | |
| CN103708678A (en) | Method for multi-stage filtration of pig farm wastewater and for recovery of nutrients | |
| CN211631399U (en) | Fish and vegetable symbiotic system based on industrial circulating mariculture | |
| CN105198088A (en) | Landscape purification steel structure tree | |
| CN107098478A (en) | A kind of microalgae carbon sequestration and sewage ecological treatment system | |
| CN102453685B (en) | Method for culturing marine green alga accumulated starch with carbon dioxide | |
| CN207404942U (en) | A kind of microalgae carbon sequestration and sewage ecological treatment system | |
| CN206767809U (en) | Biogas slurry treatment system with vegetable hydroponic cooperative disposal is absorbed based on CO2 | |
| CN104830665A (en) | Method and special device for cultivating spirulina | |
| CN106754385B (en) | Method for cultivating chlorella phytoplankton by using cyanobacterial bloom as raw material | |
| CN206284145U (en) | A kind of fish and vegetable symbiotic system | |
| CN110066737A (en) | Microalgae and the method for obtaining microalgae biomass | |
| CN102311923B (en) | Microalgae cultivation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160420 |