CN106865779A - A kind of ion type rareearth ammonia nitrogen waste water bioremediation - Google Patents
A kind of ion type rareearth ammonia nitrogen waste water bioremediation Download PDFInfo
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- CN106865779A CN106865779A CN201710211783.1A CN201710211783A CN106865779A CN 106865779 A CN106865779 A CN 106865779A CN 201710211783 A CN201710211783 A CN 201710211783A CN 106865779 A CN106865779 A CN 106865779A
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- waste water
- ammonia nitrogen
- spirulina
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/16—Nature of the water, waste water, sewage or sludge to be treated from metallurgical processes, i.e. from the production, refining or treatment of metals, e.g. galvanic wastes
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Abstract
The present invention provides a kind of ion type rareearth ammonia nitrogen waste water bioremediation, belongs to field of environment engineering technology.The method is diluted with water to ammonia nitrogen concentration not higher than 90mg/L according to the ammonia nitrogen concentration of ion type rareearth waste water, adds the nutriment in addition to ammonia nitrogen, it is configured to RE waste water Spirulina culture medium, to 560nm light absorption values for 0.2~0.5, pH is adjusted to 9~11, light regime is 12h to inoculation spirulina:12h, 30~40 DEG C of temperature is ventilated 4 times with air pump daily, 1~3 minute every time, 0.001~0.004g/L sodium acid carbonates are supplemented daily, daily light absorption value at detection spirulina liquid 560nm, when 560nm light absorption values are more than 1.1 or so, stop culture, filtering harvests spirulina.This method not only effectively reduces ammonia nitrogen concentration in waste water, makes it possible to qualified discharge, and can effectively reduce spirulina breeding cost, turn waste into wealth, the processing cost of ammonia nitrogen waste water is significantly reduced, and pollution is not resulted in environment, reduce the aquaculture cost of spirulina.
Description
Technical field
The present invention relates to field of environment engineering technology, a kind of ion type rareearth ammonia nitrogen waste water biological treatment side is particularly related to
Method.
Background technology
The main extraction process of ion type rareearth mine be mainly ammonium sulfate leach-neutralize removal of impurities-ammonium bicarbonate precipitation-
Calcination-mixed rare-earth oxide, rare-earth smelting separates corporate boss will be using acidic extractant extraction carbonated rare earth-acid back extraction
Take-ammonium hydrogen carbonate or oxalic acid precipitation-calcination obtain single rare earth oxide.Therefore, ammonia nitrogen waste water is the master of Rare Earth Production enterprise
Waste water, rare earth ammonia nitrogen wastewater flow rate is wanted to account for the 60%~70% of rare-earth wet method manufacturing enterprise waste water.Ammonia nitrogen concentration is too high in waste water,
Abundant nutriment is formed in water, the ecological environment of water quality and this area is produced serious influence and endangered, destroy water
Biological chain in body, causes water quality to enter vicious circle.Meanwhile, the ammonia nitrogen in water enters skin, respiratory tract and the alimentary canal of people
Poisoning can be caused, in 0.lmg/L, people can feel stimulation to ammonia nitrogen concentration, and concentration may crisis life in 0.7mg/L.
In order to promote economy and harmonious development, National Environmental portion has promulgated rare-earth industry pollutant emission standard《Rare-earth industry is dirty
Dye thing discharge standard》GB26451-2011, to ammonia nitrogen and other poisonous and harmful ions for example fluorine, lead, arsenic, chromium, zinc, etc. discharge propose
Requirements at the higher level, by taking ammonia nitrogen as an example, the ammonia nitrogen maximum concentration that the total floss hole of Rare Earth Production enterprise is specified in standard is 15mg/L,
Run in and put maximum concentration for 50mg/L.
The processing method of current rare earth ammonia nitrogen waste water mainly has：Steam stripping method, evaporation, bioanalysis (are divided into activity again
Sludge and biological contact oxidation process), air stripping, ion-exchange, chemical oxidization method and membrane technology (i.e. liquid film separation) etc..
Break point chlorination is swift in response, and required equipment investment is few, but liquid chlorine safe handling and shelf appeal are high, and is processed into
This height, is not suitable for the treatment of big yield high-concentration ammonia nitrogenous wastewater；Ion-exchange be applied to middle low concentration ammonia nitrogen waste water (<
500mg/L), for the ammonia nitrogen waste water of high concentration, resin regeneration frequently causes operating difficulties, and regenerated liquid still needs to further place
Reason, thus be difficult to be industrially applied in rare earth ammonia nitrogen wastewater treatment；Vaporizing extract process technique need to adjust pH, and alkali consumption is big, and have a large amount of heavy
Form sediment, except ammonia efficiency is substantially reduced during low temperature, it is adaptable to high-concentration ammonia nitrogenous wastewater treatment, operating condition requirement is high, stripping gas needs
Treatment is up to standard could to discharge；Chemical precipitation method nitric efficiency is high, process is simple, in the magnesium ammonium phosphate of generation precipitation containing N, P,
Mg, can reclaim as fertilizer and use, but precipitating reagent consumption is big, processing cost is high, is difficult to apply in actual production.Biochemical process
The RE waste water of various concentration ammonia nitrogens, strong adaptability, low cost can be processed；But strain domestication starts, and the cycle is long, strain training
Support and require height, it is difficult to control, if operation is improper, water outlet Nitrite Nitrogen can be caused to raise, cause bigger environmental hazard.
Therefore exploiting economy, environmental protection, efficient ammonia nitrogen waste water treatment method therefore needs are needed to develop a kind of low suitable for treatment
The processing method that is simple, economic, not producing secondary pollution of concentration ammonia nitrogen wastewater.
Microalgae can process food industry sewage, aquaculture wastewater, fermentation sewage, municipal wastewater, papermaking wastewater, weaving dirt
Water, chemical fertilizer factory's sewage etc., to the clearance of the ammonia nitrogen in waste water up to more than 90%.
Spirulina contains multivitamin, protein, fat, carbohydrate, mineral matter and trace element, ratio science
Rationally, with extensive edible, health care and medical value, human metabolism can be promoted, improves immunity of organisms, anti-spoke
Penetrate, aid in cancer Radiotherapy chemotherapy, reduce the effect such as radiotherapy, side effects of chemotherapy, be a kind of preferable pure natural health-care, joined
He Guo food and agricultural organizations and World of Food association of the United Nations are recommended as " 21st century optimal food ".
Using ion type rareearth ammonia nitrogen waste water cultivating spirulina, the ammonia nitrogen ion in waste water can be by spirulina as nitrogen source
Utilize, be converted into biomass, Spirullina can generate oxygen in autotroph, growth course using carbon dioxide, not only right
Environment is safe from harm, and can increase the oxygen content in local air.Therefore ion type rareearth is processed based on spirulina breeding
Ammonia nitrogen waste water have the advantages that low production cost, it is environment-friendly, turn waste into wealth, there is important meaning to local enviromental protection and improvement
Justice.
The content of the invention
The present invention is for current ion type rareearth ammonia nitrogen waste water treatment method or there is high cost, generation secondary pollution, difficulty
A kind of problem of the shortcomings for the treatment of Low Concentration Ammonia Containing Wastewater, there is provided ion type rareearth ammonia nitrogen waste water bioremediation.
The method comprises the following steps：
(1) detect that the ammonia nitrogen of ion type rareearth ammonia nitrogen waste water is dense with Berthelot spectrophotometry (HJ 535-2009)
Degree, by ion type rareearth ammonia nitrogen waste water according to the maximum tolerance ammonia nitrogen concentration dilution of spirulina, removes in addition Spirulina culture medium
Other nutritions outside nitrogen source, are configured to ion type rareearth ammonia nitrogen waste water culture medium, and medium pH is adjusted with dilute alkaline soln
To 9~11；
(2) spirulina kind is linked into the ion type rareearth ammonia nitrogen waste water culture medium of configuration in step (1), inoculum concentration is
Spirulina liquid 560nm light absorption values are 0.2~0.5；
(3) the ion type rareearth ammonia nitrogen waste water culture medium of inoculation spirulina kind in step (2) is put into illumination box
Illumination cultivation, condition of culture is：Temperature：30~40 DEG C, intensity of illumination：3000~4000lx, light regime is 12h:12h, often
It is ventilated 4 times with air pump, 1~3 minute every time, and 0.001~0.004g/L sodium acid carbonates are supplemented daily；
(4) when the 560nm light absorption values of spirulina liquid are more than 1.1, stop culture, filter or be harvested by centrifugation spirulina mud,
Filtrate with the ammonia nitrogen concentration of Berthelot spectrophotometry (HJ 535-2009) detection process water, if the waste water after treatment is expired
The ammonia nitrogen discharge standard of sufficient RE waste water, discharge after other restriction discharge indexs of waste water are qualified；If the ammonia nitrogen in waste water after treatment
Concentration is more than wastewater discharge standard, then the ammonia nitrogen concentration according to processed waste water supplements new RE waste water, and extra-nutrition thing is matched somebody with somebody
Rare earth ammonia nitrogen wastewater medium processed, repeats the above steps, and continues cultivating spirulina.Finally, by fresh spirulina freeze-drying or
It is spray dried to spirulina powder.
Wherein, the maximum tolerance ammonia nitrogen concentration of spirulina is 180mg/L.
Spirulina used is blunt top spirulina.
The culture of spirulina open container, outdoor raceway pond, illumination is provided by natural light in step (3).
Above-mentioned technical proposal of the invention has the beneficial effect that：
This method not only effectively reduces ammonia nitrogen concentration in waste water, makes it possible to qualified discharge, and can effectively reduce
Spirulina breeding cost, turns waste into wealth, and significantly reduces the processing cost of ammonia nitrogen waste water, and pollution is not resulted in environment,
Reduce the aquaculture cost of spirulina.And the method is simple for process, environment-friendly, be suitable to process ion type rareearth on a large scale
Ammonia nitrogen waste water.
Brief description of the drawings
Fig. 1 is the inventive method process chart；
Fig. 2 is the growing state of the ionic RE waste water culture spirulina of the embodiment of the present invention 1；
Fig. 3 is the growing state of the ionic RE waste water culture spirulina of the embodiment of the present invention 2；
Fig. 4 is the growing state of the ionic RE waste water culture spirulina of the embodiment of the present invention 3；
Fig. 5 is the growing state of the ionic RE waste water culture spirulina of the embodiment of the present invention 4.
Specific embodiment
To make the technical problem to be solved in the present invention, technical scheme and advantage clearer, below in conjunction with accompanying drawing and tool
Body embodiment is described in detail.
The present invention provides a kind of ion type rareearth ammonia nitrogen waste water bioremediation, and its technological process is as shown in Figure 1.
Embodiment 1
1) ammonia nitrogen concentration for detecting Longnan weight yttrium element waste water with Berthelot spectrophotometry (HJ535-2009) is
2.6g/L, 30mg/L is adjusted to running water by the ammonia nitrogen concentration of RE waste water, according to SPIRULINA CULTIVATION based formulas add except
Other nutritions of urea and sodium nitrate, urea and sodium nitrate are substituted with the ammonia nitrogen in waste water.
2) pH of the culture medium that will be prepared with sig water is adjusted to 9.2.
3) Spirulina culture medium is prepared with running water, it is 9.2 to adjust medium pH with sig water, other conditions and uses ion
Type RE waste water culture medium is identical.
4) to containing 2 liters of ionic RE waste water culture mediums and with running water prepare culture medium open-top receptacle in add spiral shell
Rotation algae kind, the A560nm for controlling initial spirulina is 0.4.
5) open-top receptacle containing spirulina kind is placed in illumination box, is in condition of culture：30 ± 1 DEG C of temperature,
Intensity of illumination：3000lx light regimes are that illumination and dark are each 12 hours, are inflated 4 times, every time 1 minute with air pump daily.
6) 2 grams of sodium acid carbonates are supplemented daily, daily the light absorption value of the 560nm of detection spirulina liquid, when spirulina liquid
A560nm is 1.1 or so stopping cultures, and the growing state of ion type rareearth waste water culture spirulina is as shown in Figure 2.
7) filtered after culture terminates and obtain spirulina mud and treated waste water, spirulina mud obtains spiral with freeze-drying
Algae powder, treats to obtain waste water Berthelot spectrophotometry (HJ 535-2009) detection ammonia nitrogen concentration, as a result shows by 4
It treatment, the ammonia nitrogen concentration in waste water is reduced to 2.3mg/L, meets RE waste water ammonia nitrogen discharge standard.
Embodiment 2
1) ammonia nitrogen concentration of RE waste water is adjusted to 60mg/L with running water, is added according to SPIRULINA CULTIVATION based formulas and removed
Other nutritions of urea and sodium nitrate, urea and sodium nitrate are substituted with the ammonia nitrogen in waste water.
2) pH of the culture medium that will be prepared with sig water is adjusted to 9.5.
3) Spirulina culture medium is prepared with running water, it is 9.5 to adjust medium pH with sig water, other conditions and uses ion
Type RE waste water culture medium is identical.
4) to containing 2 liters of ionic RE waste water culture mediums and with running water prepare culture medium open-top receptacle in add spiral shell
Rotation algae kind, the A560nm for controlling initial spirulina is 0.4.
5) open-top receptacle containing spirulina kind is placed in illumination box, is in condition of culture：35 ± 1 DEG C of temperature,
Intensity of illumination：3500lx light regimes are that illumination and dark are each 12 hours, are inflated 4 times, every time 1 minute with air pump daily.
6) 2 grams of sodium acid carbonates are supplemented daily, daily the light absorption value of the 560nm of detection spirulina liquid, when spirulina liquid
A560nm is 1.1 or so stopping cultures, and the growing state of ion type rareearth waste water culture spirulina is as shown in Figure 3.
7) filtered after culture terminates and obtain spirulina mud and treated waste water, spirulina mud obtains spiral with freeze-drying
Algae powder, treats to obtain waste water Berthelot spectrophotometry (HJ 535-2009) detection ammonia nitrogen concentration, as a result shows by 4
It treatment, the ammonia nitrogen concentration in waste water is reduced to 4.3mg/L, meets RE waste water ammonia nitrogen discharge standard.
Embodiment 3
1) ammonia nitrogen concentration of RE waste water is adjusted to 90mg/L with running water, is added according to SPIRULINA CULTIVATION based formulas and removed
Other nutritions of urea and sodium nitrate, urea and sodium nitrate are substituted with the ammonia nitrogen in waste water.
2) pH of the culture medium that will be prepared with sig water is adjusted to 10.
3) Spirulina culture medium is prepared with running water, it is 10 to adjust medium pH with sig water, other conditions and uses ion
Type RE waste water culture medium is identical.
4) to containing 2 liters of ionic RE waste water culture mediums and with running water prepare culture medium open-top receptacle in add spiral shell
Rotation algae kind, the A560nm for controlling initial spirulina is 0.4.
5) open-top receptacle containing spirulina kind is placed in illumination box, is in condition of culture：35 ± 1 DEG C of temperature,
Intensity of illumination：4000lx light regimes are that illumination and dark are each 12 hours, are inflated 4 times, every time 1 minute with air pump daily.
6) 2 grams of sodium acid carbonates are supplemented daily, daily the light absorption value of the 560nm of detection spirulina liquid, when spirulina liquid
A560nm is 1.1 or so stopping cultures, and the growing state of ion type rareearth waste water culture spirulina is as shown in Figure 4.Can be with from Fig. 4
Find out, 90mg/L ammonia nitrogen ion pair growth of spirulina platensis does not significantly inhibit effect.
7) filtered after culture terminates and obtain spirulina mud and treated waste water, spirulina mud obtains spiral with freeze-drying
Algae powder, treats to obtain waste water Berthelot spectrophotometry (HJ 535-2009) detection ammonia nitrogen concentration, as a result shows by 4
It treatment, the ammonia nitrogen concentration in waste water is reduced to 12.3mg/L, it is impossible to meet RE waste water ammonia nitrogen discharge standard.
Embodiment 4
1) ammonia nitrogen concentration of RE waste water is adjusted to 120mg/L with running water, is added according to SPIRULINA CULTIVATION based formulas
Except other nutritions of urea and sodium nitrate, urea and sodium nitrate are substituted with the ammonia nitrogen in waste water.
2) pH of the culture medium that will be prepared with sig water is adjusted to 9.5.
3) Spirulina culture medium is prepared with running water, it is 9.5 to adjust medium pH with sig water, other conditions and uses ion
Type RE waste water culture medium is identical.
4) to containing 2 liters of ionic RE waste water culture mediums and with running water prepare culture medium open-top receptacle in add spiral shell
Rotation algae kind, the A560nm for controlling initial spirulina is 0.4.
5) open-top receptacle containing spirulina kind is placed in illumination box, is in condition of culture：40 ± 1 DEG C of temperature,
Intensity of illumination：4000lx light regimes are that illumination and dark are each 12 hours, are inflated 4 times, every time 1 minute with air pump daily.
6) 2 grams of sodium acid carbonates are supplemented daily, daily the light absorption value of the 560nm of detection spirulina liquid, when spirulina liquid
A560nm is 1.1 or so stopping cultures, and the growing state of ion type rareearth waste water culture spirulina is as shown in Figure 5.Can be with from Fig. 5
Find out, 120mg/L ammonia nitrogen ion pair growth of spirulina platensis has slight inhibitory action.
7) filtered after culture terminates and obtain spirulina mud and treated waste water, spirulina mud obtains spiral with freeze-drying
Algae powder, treats to obtain waste water Berthelot spectrophotometry (HJ 535-2009) detection ammonia nitrogen concentration, as a result shows by 4
It treatment, the ammonia nitrogen concentration in waste water is reduced to 30.3mg/L, it is impossible to meets the ammonia nitrogen discharge standard of RE waste water, can mend
The RE waste water culture medium that ammonia nitrogen concentration is 60mg/L is configured to after filling RE waste water, for spirulina breeding.Or prepare dilute
Make ammonia nitrogen concentration not higher than 90mg/L during native wastewater medium.
The above is the preferred embodiment of the present invention, it is noted that for those skilled in the art
For, on the premise of principle of the present invention is not departed from, some improvements and modifications can also be made, these improvements and modifications
Should be regarded as protection scope of the present invention.
Claims (4)
1. a kind of ion type rareearth ammonia nitrogen waste water bioremediation, it is characterised in that：Comprise the following steps：
(1) ion type rareearth ammonia nitrogen waste water is added into Spirulina culture medium according to the maximum tolerance ammonia nitrogen concentration dilution of spirulina
In other nutritions in addition to nitrogen source, be configured to ion type rareearth ammonia nitrogen waste water culture medium, adjustment medium pH to 9~
11；
(2) spirulina kind is linked into the ion type rareearth ammonia nitrogen waste water culture medium of configuration in step (1), inoculum concentration is spiral
Algae solution 560nm light absorption values are 0.2~0.5；
(3) the ion type rareearth ammonia nitrogen waste water culture medium of inoculation spirulina kind in step (2) is put into illumination box illumination
Cultivate, condition of culture is：Temperature：30~40 DEG C, intensity of illumination：3000~4000lx, light regime is 12h:12h, uses daily
Air pump is ventilated 4 times, 1~3 minute every time, and 0.001~0.004g/L sodium acid carbonates are supplemented daily；
(4) when the 560nm light absorption values of spirulina liquid are more than 1.1, stop culture, filter or be harvested by centrifugation spirulina mud, chemically examine
The ammonia nitrogen concentration of processed waste water, if the waste water after treatment meets the ammonia nitrogen discharge standard of RE waste water, waste water other restrictions
Discharge after discharge index is qualified；If the ammonia nitrogen in waste water concentration after treatment is more than wastewater discharge standard, according to processed waste water
Ammonia nitrogen concentration supplement new RE waste water, extra-nutrition thing is prepared rare earth ammonia nitrogen wastewater medium, is repeated the above steps, after
Continuous cultivating spirulina.
2. ion type rareearth ammonia nitrogen waste water bioremediation according to claim 1, it is characterised in that：The spirulina
Maximum tolerance ammonia nitrogen concentration be 180mg/L.
3. ion type rareearth ammonia nitrogen waste water bioremediation according to claim 1, it is characterised in that：The spirulina
It is blunt top spirulina.
4. ion type rareearth ammonia nitrogen waste water bioremediation according to claim 1, it is characterised in that：The step
(3) culture of spirulina open container, outdoor raceway pond, illumination is provided by natural light in.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109161684A (en) * | 2018-11-12 | 2019-01-08 | 江西理工大学 | A method of utilizing spirulina recovering rare earth |
| CN111592180A (en) * | 2020-05-20 | 2020-08-28 | 上海化学工业区中法水务发展有限公司 | Method for treating organic matters in petrochemical wastewater by using spirulina |
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| DE102007035707A1 (en) * | 2007-07-30 | 2009-02-05 | GMBU Gesellschaft zur Förderung von Medizin-, Bio- und Umwelttechnologien e.V. | Method for oxygenization and pollutant absorption in aquatic systems, comprises cultivating micro algae in photo bioreactors, separating and concentrating the cells, immobilizing concentrate in polymer carriers, and collecting aggregates |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109161684A (en) * | 2018-11-12 | 2019-01-08 | 江西理工大学 | A method of utilizing spirulina recovering rare earth |
| CN109161684B (en) * | 2018-11-12 | 2020-07-24 | 江西理工大学 | Method for recovering rare earth by using spirulina |
| CN111592180A (en) * | 2020-05-20 | 2020-08-28 | 上海化学工业区中法水务发展有限公司 | Method for treating organic matters in petrochemical wastewater by using spirulina |
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