CN108192889A - A kind of method of bacteria cellulose immobilized microalgae processing waste water - Google Patents
A kind of method of bacteria cellulose immobilized microalgae processing waste water Download PDFInfo
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- CN108192889A CN108192889A CN201810027453.1A CN201810027453A CN108192889A CN 108192889 A CN108192889 A CN 108192889A CN 201810027453 A CN201810027453 A CN 201810027453A CN 108192889 A CN108192889 A CN 108192889A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
- C12N11/12—Cellulose or derivatives thereof
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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Abstract
The present invention relates to a kind of bacteria cellulose immobilized microalgae processing waste water method, including：By microalgae appropriate illumination and at a temperature of after High Density Cultivation, later by acetobacter xylinum culture medium in the culture solution of certain proportion access microalgae, acetobacter xylinum can generate cellulose trapping microalgae during growth and immobilization forms bulk or spherical, and the microalgae agglomerate of immobilization can be used to handle various waste water.Method of the present invention introduces the immobilization technology of bacteria cellulose trapping microalgae, carries out wastewater treatment, and having handled the immobilized microalgae agglomerate after waste water can recycle, and meets the requirement of microalgae industryization processing wastewater application；The present invention is suitable for various wastewater treatments, and price is more cheap, and easy to operate, and conducive to wastewater treatment, this is the new way of an economically and efficiently microalgae sewage disposal processed.
Description
Technical field
The invention belongs to technical field of environmental management, are related to a kind of side of bacteria cellulose immobilized microalgae processing waste water
Method.
Background technology
With the development of social process of industrialization, a large amount of industrial wastewaters, breeding wastewater and sanitary sewage fail to be had
Effect processing is just directly discharged into natural environment, and grave danger is caused to ecological environment.Compared with conventional method, handled using microalgae useless
Water can overcome the easily caused secondary pollution of conventional waste water processing method, potential nutriment loss, resource that cannot be fully utilized
Etc. drawbacks, while can effectively and at low cost remove the nutriments such as the nitrogen for causing body eutrophication, phosphorus, therefore with wide
Wealthy application prospect.
Compared with free algae, the area of space progress that free cell is positioned to restriction using physics and chemical means is micro-
The immobilization of algae so that microalgae is with cell density is high, reaction speed is fast, load-bearing capacity is strong, stable and reliable operation, is easy to solid-liquid
The features such as separation, moreover it is possible to and the catabolic activity of algae is reduced to a certain extent, and it is made to keep bioactivity simultaneously can be anti-
It is multiple to utilize.
Traditional immobilized microalgae technology mainly includes absorption method and investment.Absorption is that frustule is attached to carrier table
Face, and it is then to embed frustule or be enclosed in carrier inside to embed, obtained carrier used is mainly by organic and inorganic high score
Sub- material etc..The synthesis cost of these carriers is higher, and preparation procedure is complicated, needs to centrifuge, rinse etc. compared with multi-step, using one
After fixing time, the growth of frustule cause frustule come off or the rupture of immobilization algae ball, these unfavorable factors are in certain journey
The application of immobilized microalgae is affected on degree.
The present invention traps microalgae and conduct using the bacteria cellulose that the acetobacter xylinum that nature is widely present generates
Carrier realizes immobilization, has carried out the research of bacteria cellulose immobilized microalgae processing waste water.This method is simple and efficient, at low cost
It is honest and clean, greatly reduce the cost of microalgae processing waste water.
Invention content
In view of this, the purpose of the present invention is to provide a kind of bacteria cellulose immobilized microalgae processing waste water method,
It bacteria cellulose is generated by acetobacter xylinum traps microalgae and form big bulk or spherical, be simple and efficient so as to develop one kind,
Low-cost immobilized microalgae handles waste water technology.
In order to reach above-mentioned purpose, the present invention provides a kind of sides of bacteria cellulose immobilized microalgae processing waste water
Method includes the following steps：
(1) High Density Cultivation of microalgae；
(2) culture of acetobacter xylinum；
(3) with certain proportion add acetobacter xylinum culture medium to micro algae culturing liquid in and the good acetobacter xylinum of inoculated and cultured,
Acetobacter xylinum generates bacteria cellulose trapping and immobilized microalgae；
(4) microalgae cell of immobilization is used for handling waste water.
Further, wherein the High Density Cultivation of microalgae described in step (1) specifically includes：Microalgae 3000~
5000lux intensities of illumination after expanding culture under conditions of 20~28 DEG C, are seeded to culture apparatus culture, until cell log is given birth to
For a long time, cell density reaches 106~1010Until above.The cultural method can be autotrophy culture or Heterotrophic culture, it is therefore an objective to be
Obtain highdensity algae solution.Microdisk electrode is since microalgae density at this time is high, activity is strong, after immobilization to logarithmic phase
It is good to manage waste water effect, algae solution is accessed after acetobacter xylinum culture to exponential phase and is trapped, generation cellulose vigor is stronger, has
Rapid acquiring and fixation conducive to microalgae.
Further, wherein the culture apparatus is selected from shaking flask, ventilation bottle, bioreactor, fermentation tank or open training
Support one kind in pond.
Further, wherein the microalgae includes but not limited to Chlorella (chlorella sp.), barrel mast Trentepohlia
(cy1indrotheca sp.), diatom (diatom), diamond shape algae (Nitzschia sp.) split pot algae (schizochytrium
Sp.), scenedesmus (Scenedesmus sp.), Nannochloropsis oculata (Nannochloris sp.), Chlamydomonas (Chlamydomonas
Sp.), flat algae (Tetraselmis sp.), it is a kind of or these kind microalgaes mixed in empty ball Trentepohlia (Eudorina sp.)
Close object etc..
Further, wherein the culture medium of the microalgae includes but not limited to BG-11 culture mediums, SE culture mediums, BBM cultures
Base or one kind in TAP culture mediums and other any modified micro-algae culture mediums and its Heterotrophic culture base.
Further, wherein the condition of culture of acetobacter xylinum described in step (2) is：25-28 DEG C, 130r/min, culture 5
My god.
Further, wherein the formula of the acetobacter xylinum culture medium is as follows:30g/l oligofructose, 20g/l yeast extracts
(powder), 0.4g/l anhydrous magnesium sulfates, 3.3g/l ammonium sulfate, 1g/l potassium dihydrogen phosphates, 1.75g/l monohydrate potassiums, 2.4g/l
Trisodium citrate dihydrate originates pH4.6.
Further, wherein step (3) specifically includes：By acetobacter xylinum culture medium with the ratio of 10%~50% (v/v)
Acetobacter xylinum culture medium is added in the micro algae culturing liquid that step (1) obtains, and inoculation step (2) cultured acetobacter xylinum,
Condition of culture is controlled, until acetobacter xylinum generates bacteria cellulose trapping microalgae and forms bulk or spherical, with simple separation side
Method carries out microalgae harvesting.
Further, wherein step (3) specifically includes, and acetobacter xylinum culture is added with the ratio of 10%~40% (v/v)
In base to micro algae culturing liquid, and the acetobacter xylinum of 1%~5% (v/v) is inoculated with, in 24~30 DEG C, 0~80 rev/min of condition
It is lower oscillation or stirring 4~48 hours after, acetobacter xylinum generate bacteria cellulose trapping microalgae simultaneously form bulk or spherical, by beating
The method of fishing or filtering obtains the immobilized microalgae material using bacteria cellulose as carrier.
Further, wherein waste water described in step (5) includes but not limited to municipal wastewater, industrial wastewater (such as brewery
Waste water and waste water of paper mill), one kind in breeding wastewater (such as pig-farm wastewater) or biogas fermentation waste water.
Further, wherein step (5) specifically includes：By immobilized microalgae agglomerate be thrown to that microalgae is suitable for it is various useless
In water (different microalgaes has different characteristics that can handle different waste water), the immobilized microalgae agglomerate at certain temperature and illumination
Its photosynthetic activity can be kept, has and preferably denitrogenates phosphorus and the effect of COD, while also there is higher adsorptivity.
The invention has the advantages that：
Method of the present invention is at low cost, and immobilized microalgae, which handles waste water, to be recycled, and safe
It is nontoxic.As long as can generate, the acetobacter of bacteria cellulose can trap and immobilized microalgae handles waste water, apply for human hair
Existing fast growing, the high acetobacter of bacteria cellulose output can obtain good effect, and control bacteria cellulose is fixed
Changing the degree of microalgae can make microalgae keep metabolic activity, and the tensile strength of immobilization aggregate is larger, is not easily broken, and frond is not
Easy to fall off, stable and reliable operation can be used for carrying out wastewater treatment.
Method of the present invention introduces the immobilization technology of bacteria cellulose trapping microalgae, carries out wastewater treatment, place
Having managed the immobilization frustule after waste water can recycle, and meet the requirement of microalgae industryization processing wastewater application；And this
Invention is suitable for various wastewater treatments, and price is more cheap, and easy to operate, conducive to wastewater treatment, this be an economy,
The efficiently new way of microalgae sewage disposal processed.
Description of the drawings
Figure 1A is the light microscopic figure that bacteria cellulose traps immobilization scenedesmus obliquus agglomerate；
Figure 1B is the scanning electron microscope (SEM) photograph that bacteria cellulose traps immobilization scenedesmus obliquus agglomerate；
Fig. 2A is that bacteria cellulose traps the treatment effect that immobilization scenedesmus obliquus frustule removes high-concentration culture waste water
One of figure；
Fig. 2 B are that bacteria cellulose traps the treatment effect that immobilization scenedesmus obliquus frustule removes high-concentration culture waste water
The two of figure；
Fig. 2 C are that bacteria cellulose traps the treatment effect that immobilization scenedesmus obliquus frustule removes high-concentration culture waste water
The three of figure；
Fig. 2 D are that bacteria cellulose traps the treatment effect that immobilization scenedesmus obliquus frustule removes high-concentration culture waste water
The four of figure.
Specific embodiment
The following example will be further illustrated other features and advantages of the present invention, but such embodiment it is merely illustrative and
With not limitation of the present invention.
Example 1：The immobilization processing waste water of chlorella
The SE culture mediums for cultivating chlorella are as follows：NaNO30.25g/L；K2HPO4-3H2O 0.075g/L；MgSO4-7H2O
0.075g/L；CaCl2-2H2O 0.025g/L；KH2PO40.175g/L；NaCl 0.025g/L；FeCl3-6H2O 0.005g/L；
EDTA-Fe 1mL；A51mL；Soil extract 40mL.
Soil extract (Soil extract) is prepared：Appropriate garden soil (not applying overfertilization) is put into baking oven, 60 DEG C of bakings
It is dry to stay overnight, and its 250g is claimed to pour into 3000mL triangular flasks, add 1000mL distilled water, bottleneck is sealed with porous plug, in a water bath
Boiling water heats 3h, cools down precipitates overnight, and aseptically 800-1000rpm/min centrifuges 5min, takes supernatant, and sterilizing is steamed
Distilled water add in supernatant to 1000 milliliters of volume, soil extract be stored in 4 DEG C it is spare.
EDTA-Fe solution：4.1ml concentrated hydrochloric acids are measured to be diluted in 500ml distilled water；Separately weigh 0.9306g1N EDTA-
Na2 is dissolved in 50ml distilled water；10ml 1N HCl are measured again dissolved with 0.901g FeCl36H2O and 10ml0.1N EDTA-
Na2 is mixed, and is diluted to 1000ml.
A5(Trace mental solution) solution：H3BO32.8g/L dH2O；MnCl·4H2O 1.86g/L,
ZnSO4·7H2O 0.22g/L；Na2MoO4·2H2O 0.39g/L；CuSO4·5H2O 0.08g/L；Co(NO3)2·6H2O
0.05g/L.Heterotrophic culture based formulas is same as above, while adds in organic carbon source (such as glucose, fructose) to initial reduction sugar a concentration of 1
~15g/L.
With 1~5 × 106The inoculum concentration of a/milliliter algae cell density, chlorella is inoculated into SE culture mediums, ventilation training
It supports, in 20~30 DEG C and 30~54 μm of ol/m2It under s illumination conditions, cultivates 10 days, algae cell density is 2~6 × 107A/milli
It rises, obtains bead algae culturing liquid.For there is the chlorella of Heterotrophic culture characteristic, with 1~5 × 106A/milliliter algae cell density
Chlorella is inoculated into Heterotrophic culture base by inoculum concentration, after 25~30 DEG C of 80~120r/min of shaking table are cultivated 6 days, frustule
Density reaches 2~9 × 109A/milliliter obtains bead algae culturing liquid.By obtained bead algae culturing liquid in following examples.
Foster or 500 milliliters of the cultured bead algae culturing liquid of heterotrophism mode is derived from, adds in the acetobacter xylinum of sterilizing thereto
Culture medium 120ml, access acetobacter xylinum 6% (v/v), start after 12 hours generate agglomerate, 48 hours capture rates reach 98% with
On, you can to take out the immobilized microalgae agglomerate of cellulose trapping.The immobilized microalgae agglomerate of formation is transferred to high concentration
It in (1000~1500mg/lCOD) municipal wastewater and pig-farm wastewater 1500ml, does not need to stir, discontinuity Air Exposure.As a result
Show this bacteria cellulose trapping Immobilized cells culture group with it is efficient except ammonia, dephosphorization and except COD effect (after 6 days, ammonia
Nitrogen removal efficiency reaches more than 90%, and nitrogen removal rate reaches more than 65%, and total tp removal rate reaches more than 65%, COD removal rates
Reach more than 60%), and also there is efficient adsorptivity.
Example 2：Scenedesmus obliquus immobilization handles waste water
The SE culture mediums for cultivating scenedesmus obliquus are as follows：NaNO30.25g/L；K2HPO4-3H2O 0.075g/L；MgSO4-7H2O
0.075g/L；CaCl2-2H2O 0.025g/L；KH2PO40.175g/L；NaCl 0.025g/L；FeCl3-6H2O 0.005g/L；
EDTA-Fe 1mL；A51mL；Soil extract 40mL.
Soil extract (Soil extract) is prepared：Appropriate garden soil (not applying overfertilization) is put into baking oven, 60 DEG C of bakings
It is dry to stay overnight, and its 250g is claimed to pour into 3000mL triangular flasks, add 1000mL distilled water, bottleneck is sealed with porous plug, in a water bath
Boiling water heats 3h, cools down precipitates overnight, aseptically
800-1000rpm/min centrifuges 5min, takes supernatant, and sterile purified water is added in supernatant to 1000 milli of volume
Rise, soil extract be stored in 4 DEG C it is spare.
EDTA-Fe solution：4.1ml concentrated hydrochloric acids are measured to be diluted in 500ml distilled water；Separately weigh 0.9306g1N EDTA-
Na2 is dissolved in 50ml distilled water；10ml 1N HCl are measured again dissolved with 0.901gFeCl36H2O and 10ml0.1N EDTA-Na2
It is mixed, and is diluted to 1000ml.
A5(Trace mental solution) solution：H3BO32.8g/L dH2O；MnCl·4H2O 1.86g/L,
ZnSO4·7H2O 0.22g/L；Na2MoO4·2H2O 0.39g/L；CuSO4·5H2O 0.08g/L；Co(NO3)2·6H2O
0.05g/L。
With 15 × 106The inoculum concentration of a/milliliter algae cell density, scenedesmus obliquus is inoculated into SE culture mediums, ventilation training
It supports, in 20~30 DEG C and 30~54 μm of ol/m2Under s illumination conditions, after cultivating 10 days, algae cell density reaches 2~6 × 107
A/milliliter obtains scenedesmus obliquus culture solution.By obtained scenedesmus obliquus culture solution in following examples.
500 milliliters of scenedesmus obliquus culture solution is taken, adds in the acetobacter xylinum culture medium 110ml of sterilizing, access culture thereto
Good acetobacter xylinum 3% (v/v), starts to trap agglomerating after 4 hours, arresting efficiency reaches 95% after 8 hours, you can takes out fiber
The immobilized microalgae agglomerate (see Figure 1A, Figure 1B) of element trapping, Figure 1A are shown the microalgae agglomerate after immobilization and are seen under light microscopic
The microstate that the microalgae agglomerate after immobilization is observed under surface sweeping Electronic Speculum is shown in the state observed, Figure 1B；From Figure 1A,
It is durable non-breakable as can be seen that scenedesmus obliquus is wrapped up by aggregation, and is wound by cellulose in Figure 1B.By the immobilization
Microalgae quantity is about 2~6 × 108Agglomerate be transferred to the initial COD values of 2000ml be 1000mg/L breeding wastewater or biogas fermentation
It in waste water, does not need to stir, each hour aeration is primary, aeration quantity 200ml/min；Fig. 2A-Fig. 2 D's the results show that this
Bacteria cellulose trapping immobilization scenedesmus obliquus group with it is efficient except ammonia, dephosphorization and except COD effect (after 6 days, ammonia nitrogen removal
Rate reaches more than 95%, and nitrogen removal rate reaches more than 85%, and total tp removal rate reaches more than 70%, COD concentration removal rates and reaches
To more than 75%), and also there is efficient adsorptivity.
Example 3：Mixed algae immobilization handles waste water
SE culture mediums are prepared, with 1~5 × 106A/milliliter is respectively connected to Chlamydomonas reinhardtii, chlorella, scenedesmus obliquus each 5%,
Ventilation culture, in 20~30 DEG C and 30~54 μm of ol/m2Under s illumination conditions, after cultivating 10 days, mixing algae solution is obtained, algae is thin
Born of the same parents' density is 2~6 × 107A/milliliter.By obtained mixing algae culturing liquid in following examples.Take mixing algae culturing liquid 500
Milliliter, adds in the acetobacter xylinum culture medium 100ml of sterilizing, cultured acetobacter xylinum 5% (v/v) is accessed, after 4 hours thereto
Start to trap agglomerating, arresting efficiency reaches 96% after 12 hours, you can take out the immobilized microalgae agglomerate of cellulose trapping.It should
Immobilized microalgae quantity is about 2~6 × 109Agglomerate add in 1200mg/LCOD beer brewery water and waste water of paper mill in, often
One hour aeration is primary, and aeration quantity 200ml/min results show this bacteria cellulose trapping immobilization mixed algae agglomerate tool
Have that efficient (after 6 days, ammonia nitrogen removal frank reaches more than 95%, and nitrogen removal rate reaches except ammonia, dephosphorization and effect except COD
More than 70%, total tp removal rate reaches more than 75%, COD concentration removal rates and reaches more than 65%).
In conclusion the foregoing is merely presently preferred embodiments of the present invention, the patent for not thereby limiting the present invention is protected
Range is protected, therefore description of the invention and attached drawing make equivalence changes etc. such as, should all be included in protection scope of the present invention.
Claims (10)
- A kind of 1. method of bacteria cellulose immobilized microalgae processing waste water, which is characterized in that include the following steps：(1) High Density Cultivation of microalgae；(2) culture of acetobacter xylinum；(3) with certain proportion add acetobacter xylinum culture medium to micro algae culturing liquid in and the good acetobacter xylinum of inoculated and cultured, wooden vinegar Bacillus generates bacteria cellulose trapping and immobilized microalgae；(4) microalgae cell of immobilization is used for handling waste water.
- 2. according to the method described in claim 1, it is characterized in that, the High Density Cultivation of microalgae described in step (1) is specifically wrapped It includes：Microalgae after expanding culture under conditions of 20~28 DEG C, is seeded to culture apparatus training in the intensity of illumination of 3000~5000lux It supports, until cell log growth period, cell density reaches 106-1010Until above.
- 3. according to the method described in claim 2, it is characterized in that, the culture apparatus is anti-selected from shaking flask, ventilation bottle, photo-biological Answer one kind in device, fermentation tank or open culturing pond.
- 4. according to the method described in claim 3, it is characterized in that, the microalgae include Chlorella (chlorella sp.), Barrel mast Trentepohlia (cy1indrotheca sp.), diamond shape algae (Nitzschia sp.), splits pot algae at diatom (diatom) (schizochytrium sp.), scenedesmus (Scenedesmus sp.), Nannochloropsis oculata (Nannochloris sp.), Chlamydomonas (Chlamydomonas sp.), flat algae (Tetraselmis sp.), one kind in empty ball Trentepohlia (Eudorina sp.) or The mixture of these kind microalgaes.
- 5. according to the method described in claim 1, it is characterized in that, the culture medium of the microalgae includes BG-11 culture mediums, SE is trained Support one kind in base, BBM culture mediums or TAP culture mediums.
- 6. according to the method described in claim 1, it is characterized in that, the condition of culture of acetobacter xylinum described in step (2) is： It 25-28 DEG C, 130r/min, cultivates 5 days.
- 7. according to the method described in claim 6, it is characterized in that, the formula of the acetobacter xylinum culture medium is as follows:30g/l is low Fructooligosaccharides, 20g/l yeast extracts, 0.4g/l anhydrous magnesium sulfates, 3.3g/l ammonium sulfate, 1g/l potassium dihydrogen phosphates, 1.75g/l mono- are hydrated Citric acid, 2.4g/l trisodium citrate dihydrates originate pH4.6.
- 8. according to the method described in claim 1, it is characterized in that, step (3) specifically includes：With by acetobacter xylinum culture medium with The ratio of 10%~50% (v/v) is added in the micro algae culturing liquid that step (1) obtains, and the cultured wooden vinegar of inoculation step (2) Bacillus controls condition of culture, until acetobacter xylinum generates, bacteria cellulose trapping microalgae forms bulk or spherical immobilization is micro- Algae material carries out microalgae harvesting with simple separation method.
- 9. according to the method described in claim 8, it is characterized in that, step (3) specifically includes, by acetobacter xylinum culture medium with The ratio of 10%~40% (v/v) is added in micro algae culturing liquid, and is inoculated with the acetobacter xylinum of 1%~5% (v/v), 24~ After vibrating or stir 4~48 hours under conditions of 30 DEG C, 0~80 rev/min, acetobacter xylinum generates bacteria cellulose trapping microalgae And bulk or spherical is formed, the immobilized microalgae material using bacteria cellulose as carrier is obtained by the method salvaged or filtered.
- 10. according to the method described in claim 1, it is characterized in that, waste water described in step (5) includes municipal wastewater, industry One kind in waste water, breeding wastewater or biogas fermentation waste water.
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| CN117089544A (en) * | 2023-09-06 | 2023-11-21 | 山东悦翔生物有限公司 | Microalgae culture method based on modified cellulose carrier |
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| CN109133354B (en) * | 2018-06-25 | 2020-11-20 | 浙江大学 | Algae-based ecological bacteriostasis method for wastewater treatment |
| CN109231488A (en) * | 2018-10-19 | 2019-01-18 | 浙江海洋大学 | A kind of method of synchronization process water body nitrogen phosphorus inorganic nutrients and organic pollutant |
| CN109231488B (en) * | 2018-10-19 | 2021-06-18 | 浙江海洋大学 | Method for synchronously treating nitrogen and phosphorus inorganic nutrients and organic pollutants in water body |
| CN111018127A (en) * | 2020-02-12 | 2020-04-17 | 青岛卓越海洋集团有限公司 | Method for treating aquaculture wastewater by algae |
| CN115215506A (en) * | 2022-06-07 | 2022-10-21 | 海南省智慧环境投资控股有限公司 | Planting and breeding coupling method for purifying breeding sewage by using mangrove forest |
| CN117089544A (en) * | 2023-09-06 | 2023-11-21 | 山东悦翔生物有限公司 | Microalgae culture method based on modified cellulose carrier |
| CN117089544B (en) * | 2023-09-06 | 2024-05-14 | 山东悦翔生物有限公司 | Microalgae culture method based on modified cellulose carrier |
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