CN117089544A - Microalgae culture method based on modified cellulose carrier - Google Patents
Microalgae culture method based on modified cellulose carrier Download PDFInfo
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- CN117089544A CN117089544A CN202311139235.4A CN202311139235A CN117089544A CN 117089544 A CN117089544 A CN 117089544A CN 202311139235 A CN202311139235 A CN 202311139235A CN 117089544 A CN117089544 A CN 117089544A
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- silicon dioxide
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- 239000001913 cellulose Substances 0.000 title claims abstract description 62
- 229920002678 cellulose Polymers 0.000 title claims abstract description 62
- 238000012136 culture method Methods 0.000 title claims abstract description 9
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 83
- 239000005543 nano-size silicon particle Substances 0.000 claims abstract description 38
- 235000012239 silicon dioxide Nutrition 0.000 claims abstract description 38
- 239000000243 solution Substances 0.000 claims abstract description 30
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 27
- 239000011259 mixed solution Substances 0.000 claims abstract description 17
- 238000003756 stirring Methods 0.000 claims abstract description 12
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims abstract description 11
- 235000017491 Bambusa tulda Nutrition 0.000 claims abstract description 11
- 241001330002 Bambuseae Species 0.000 claims abstract description 11
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims abstract description 11
- 239000011425 bamboo Substances 0.000 claims abstract description 11
- 239000000835 fiber Substances 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000002156 mixing Methods 0.000 claims abstract description 9
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000004202 carbamide Substances 0.000 claims abstract description 8
- 238000006243 chemical reaction Methods 0.000 claims abstract description 8
- -1 alkyl carboxylic acid Chemical class 0.000 claims abstract description 7
- 239000003929 acidic solution Substances 0.000 claims abstract description 6
- 230000033444 hydroxylation Effects 0.000 claims abstract description 3
- 238000005805 hydroxylation reaction Methods 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims description 18
- 238000012258 culturing Methods 0.000 claims description 17
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 9
- POULHZVOKOAJMA-UHFFFAOYSA-N dodecanoic acid Chemical compound CCCCCCCCCCCC(O)=O POULHZVOKOAJMA-UHFFFAOYSA-N 0.000 claims description 8
- 230000012010 growth Effects 0.000 claims description 7
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 5
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 3
- 235000021314 Palmitic acid Nutrition 0.000 claims description 3
- 235000021355 Stearic acid Nutrition 0.000 claims description 3
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 claims description 3
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 claims description 3
- 241000227752 Chaetoceros Species 0.000 claims description 2
- 241000192701 Microcystis Species 0.000 claims description 2
- TUNFSRHWOTWDNC-UHFFFAOYSA-N Myristic acid Natural products CCCCCCCCCCCCCC(O)=O TUNFSRHWOTWDNC-UHFFFAOYSA-N 0.000 claims description 2
- 241000316848 Rhodococcus <scale insect> Species 0.000 claims description 2
- 239000003960 organic solvent Substances 0.000 claims description 2
- 239000002131 composite material Substances 0.000 abstract description 6
- 239000002994 raw material Substances 0.000 abstract description 3
- 239000010865 sewage Substances 0.000 description 11
- 244000005700 microbiome Species 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 239000002253 acid Substances 0.000 description 6
- 230000007935 neutral effect Effects 0.000 description 6
- 238000000746 purification Methods 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 230000007547 defect Effects 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 230000001376 precipitating effect Effects 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000009210 therapy by ultrasound Methods 0.000 description 3
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 238000012364 cultivation method Methods 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 238000011056 performance test Methods 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- NOQGZXFMHARMLW-UHFFFAOYSA-N Daminozide Chemical compound CN(C)NC(=O)CCC(O)=O NOQGZXFMHARMLW-UHFFFAOYSA-N 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 235000010419 agar Nutrition 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 238000010626 work up procedure Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
- C12N11/12—Cellulose or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/89—Algae ; Processes using algae
Abstract
The invention provides a microalgae culture method based on a modified cellulose carrier, which comprises the following steps: s1, carrying out hydroxylation treatment on nano silicon dioxide, and carrying out blending reaction on the nano silicon dioxide and alkyl carboxylic acid to obtain modified nano silicon dioxide; s2, blending sodium hydroxide, urea and bamboo fibers in water and stirring to obtain a cellulose solution; s3, placing the modified nano silicon dioxide into the cellulose solution, and uniformly stirring to obtain a mixed solution; s4, adding the mixed solution into an acidic solution for curing to obtain a modified cellulose carrier; s5, microalgae planting is carried out on the modified cellulose carrier. The invention takes the components of modified nano silicon dioxide, urea, cellulose and the like as raw materials to prepare the modified cellulose carrier, which has the advantages of strong stability, large specific surface area, good biological affinity and the like, and the microalgae composite material based on the carrier can purify water body efficiently and continuously and has good application prospect.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to a microalgae culture method based on a modified cellulose carrier.
Background
At present, the purification treatment of sewage and wastewater mostly adopts the means of physical separation or chemical conversion, but the method has the defects of complex treatment system, high operation cost, high energy consumption, incomplete treatment and possible secondary pollution. The method for purifying sewage by adopting microorganisms is a novel technical means, and has the advantages of low energy consumption, high efficiency, sustainability and low cost, so that the method is increasingly paid attention to.
The microalgae has the characteristics of high growth speed and short growth period, and can efficiently absorb and purify organic matters, synthetic sugar, fatty acid and other substances in water body in a light and action or abnormal transformation mode, so that the microalgae becomes one of the primary selection objects in microorganism purification.
In the technology of microorganism sewage treatment, the microorganism carrier plays roles of fixing biological membranes and adsorbing pollutants, so that the microorganism carrier is an important component in the technology of microorganism purification and repair. The existing carriers for fixing and growing microalgae microorganisms generally adopt polymers such as sodium alginate, agar, gelatin or polypropylene, however, the carriers respectively have the defects of low stability, poor biocompatibility, difficult degradation and the like, and the requirements of actual production and application cannot be met.
Based on this, a new solution is needed to solve the above-mentioned drawbacks of the prior art.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides a microalgae culture method based on a modified cellulose carrier. The invention takes the components of modified nano silicon dioxide, urea, cellulose and the like as raw materials to prepare the modified cellulose carrier, which has the advantages of strong stability, large specific surface area, good biological affinity and the like, and the microalgae composite material based on the carrier can purify water body efficiently and continuously and has good application prospect.
An object of the present invention is to provide a method for culturing microalgae based on a modified cellulose carrier, comprising the steps of:
s1, carrying out hydroxylation treatment on nano silicon dioxide, and carrying out blending reaction on the nano silicon dioxide and alkyl carboxylic acid to obtain modified nano silicon dioxide;
s2, blending sodium hydroxide, urea and bamboo fibers in water and stirring to obtain a cellulose solution;
s3, placing the modified nano silicon dioxide into the cellulose solution, and uniformly stirring to obtain a mixed solution;
s4, adding the mixed solution into an acidic solution for curing to obtain a modified cellulose carrier;
s5, microalgae planting is carried out on the modified cellulose carrier.
Further, the alkyl carboxylic acid is selected from one or more of dodecanoic acid, tetradecanoic acid, hexadecanoic acid, or octadecanoic acid.
Further, the mass ratio of the nano silicon dioxide to the bamboo fiber is 1:10-1:20.
Further, in step S4, the acidic solution is a mixed solution of acetic acid and an organic solvent.
Further, the microalgae is selected from one or more of chlorella, microcystis, chaetoceros and rhodococcus.
Further, in step S5, the microalgae is cultured to a stable growth stage before the microalgae colonization.
Further, in the step S5, the temperature of the microalgae planting is 20-25 ℃, and the illumination time is 3-6 h/day.
Further, the nano-silica has a size of 10-100 nm.
The environment-friendly laundry gel bead with high stability provided by the invention has the following beneficial effects:
according to the culture method of the microalgae based on the modified cellulose carrier, the components such as the alkyl carboxylic acid modified nano silicon dioxide and the bamboo fiber are adopted as raw materials, so that the porous gelatinous modified cellulose carrier is prepared, and as a large amount of long-chain alkyl groups are introduced into the surface of the modified nano silicon dioxide, the compatibility among various components is improved, the modified nano silicon dioxide can be stably wrapped with cellulose to form a more uniform and compact structure, the specific surface area of the carrier is increased, the growth promoting effect on the subsequent planted microalgae is good, and the purification capability of sewage is also remarkably improved.
Detailed Description
In order to more clearly illustrate the technical solution of the present invention, the following examples are set forth. The starting materials, reactions and workup procedures used in the examples are those commonly practiced in the market and known to those skilled in the art unless otherwise indicated.
The nanosilica in the examples of the present invention was purchased from alar Ding Shiji, particle size 100 nm.
The bamboo fiber in the embodiment of the invention is purchased from Mingtong bamboo charcoal products limited company, and the cellulose content is more than 98%.
The acidic solution in the embodiment of the invention comprises the following components in percentage by mass: 12% of chloroform, 32% of ethyl acetate and 56% of acetic acid.
The microalgae in the embodiment of the invention are chlorella.
The preparation method of the microalgae concentrated solution in the embodiment of the invention comprises the following steps: centrifuging microalgae in logarithmic growth phase at 5000 rpm for 15 min, removing supernatant, adding sterile water, and regulating algae cell density to 3×10 7 And (5) cell/mL to obtain microalgae concentrated solution.
Example 1
A method for culturing microalgae based on a modified cellulose carrier, the method for culturing microalgae based on a modified cellulose carrier comprises the following steps:
s1, adding nano silicon dioxide into excessive sodium hydroxide solution (the concentration is 5 mol/L), performing ultrasonic treatment on the nano silicon dioxide to obtain 3 h, washing the nano silicon dioxide to be neutral, and drying the nano silicon dioxide to obtain hydroxylated silicon dioxide;
blending the hydroxylated silicon dioxide with dodecanoic acid (1:5, m/m), carrying out ultrasonic reaction at 70 ℃ by taking ethanol as a solvent for 10 h, and then filtering and precipitating to obtain modified nano silicon dioxide;
s2, adding 7 g sodium hydroxide, 12 g urea and 10 g bamboo fibers into 100 mL water, and stirring 2 h to obtain a cellulose solution;
s3, adding the modified nano silicon dioxide of 1 g into the cellulose solution, and stirring for 1 h to obtain a mixed solution;
s4, dropwise adding the mixed solution into an acid solution (the mixed solution is acid solution=1:2, v/v) for curing for 10 min, taking out, and washing to be neutral to obtain a modified cellulose carrier;
s5, spraying microalgae concentrated solution on the surface of the modified cellulose carrier according to the dosage of 0.8 mL/g, and then adding the treated modified cellulose carrier into LB culture medium with the concentration of 0.5% according to the dosage of 800 g/L, and culturing for 3 days at 25 ℃ under 3500 lux light to obtain the product.
Example 2
A method for culturing microalgae based on a modified cellulose carrier, the method for culturing microalgae based on a modified cellulose carrier comprises the following steps:
s1, adding nano silicon dioxide into excessive sodium hydroxide solution (the concentration is 5 mol/L), performing ultrasonic treatment on the nano silicon dioxide to obtain 3 h, washing the nano silicon dioxide to be neutral, and drying the nano silicon dioxide to obtain hydroxylated silicon dioxide;
blending the hydroxylated silicon dioxide with hexadecanoic acid (1:5, m/m), carrying out ultrasonic reaction at 70 ℃ by using ethanol as a solvent for 10 h, and then filtering and precipitating to obtain modified nano silicon dioxide;
s2, adding 7 g sodium hydroxide, 12 g urea and 10 g bamboo fibers into 100 mL water, and stirring 2 h to obtain a cellulose solution;
s3, adding the modified nano silicon dioxide of 0.9-g into the cellulose solution, and stirring for 1-h to obtain a mixed solution;
s4, dropwise adding the mixed solution into an acid solution (the mixed solution is acid solution=1:2, v/v) for curing for 10 min, taking out, and washing to be neutral to obtain a modified cellulose carrier;
s5, spraying microalgae concentrated solution on the surface of the modified cellulose carrier according to the dosage of 0.8 mL/g, and then adding the treated modified cellulose carrier into LB culture medium with the concentration of 0.5% according to the dosage of 800 g/L, and culturing for 3 days at 25 ℃ under 3500 lux light to obtain the product.
Example 3
A method for culturing microalgae based on a modified cellulose carrier, the method for culturing microalgae based on a modified cellulose carrier comprises the following steps:
s1, adding nano silicon dioxide into excessive sodium hydroxide solution (the concentration is 5 mol/L), performing ultrasonic treatment on the nano silicon dioxide to obtain 3 h, washing the nano silicon dioxide to be neutral, and drying the nano silicon dioxide to obtain hydroxylated silicon dioxide;
blending the hydroxylated silicon dioxide with octadecanoic acid (1:5, m/m), carrying out ultrasonic reaction at 70 ℃ by using ethanol as a solvent for 10 h, and then filtering and precipitating to obtain modified nano silicon dioxide;
s2, adding 7 g sodium hydroxide, 12 g urea and 10 g bamboo fibers into 100 mL water, and stirring 2 h to obtain a cellulose solution;
s3, adding the modified nano silicon dioxide of 0.8-g into the cellulose solution, and stirring for 1-h to obtain a mixed solution;
s4, dropwise adding the mixed solution into an acid solution (the mixed solution is acid solution=1:2, v/v) for curing for 10 min, taking out, and washing to be neutral to obtain a modified cellulose carrier;
s5, spraying microalgae concentrated solution on the surface of the modified cellulose carrier according to the dosage of 0.8 mL/g, and then adding the treated modified cellulose carrier into LB culture medium with the concentration of 0.5% according to the dosage of 800 g/L, and culturing for 3 days at 25 ℃ under 3500 lux light to obtain the product.
Comparative example 1
A microalgae cultivation method is different from example 1 in that: in step S3, the modified nanosilica was replaced with unmodified nanosilica by equal mass, and the other components and steps were the same as in example 1.
Comparative example 2
A microalgae cultivation method is different from example 1 in that: in step S1, butyric acid was used instead of dodecanoic acid to obtain butyric acid-modified nanosilica, and other components and steps were the same as in example 1.
Test case
The microalgae-cellulose carrier composite materials prepared in example 1 and comparative examples 1-2 were subjected to a sewage purification performance test.
The testing method comprises the following steps:
the microalgae-cellulose carrier composite materials prepared in the example or the comparative example are respectively put into the bottoms of different sewage tanks, the input amount of the microalgae-cellulose carrier composite materials is 20 percent wt percent of the sewage, aeration is carried out for 15 days, the oxygen content in the sewage is controlled to be 5 mg/L, the illumination intensity is 1000 lux, periodic illumination is carried out according to illumination 4 h and darkness 8 h, the temperature is controlled to be 28 ℃, the pH is 7.5, and the concentration of pollutants in the sewage before and after treatment is detected.
The test results are shown in Table 1.
TABLE 1 Performance test results
According to the table 1, the microalgae culture method based on the modified cellulose carrier effectively promotes the growth and enrichment of microalgae, and the obtained microalgae-cellulose carrier composite material can efficiently and stably treat harmful components in sewage and has good purifying capacity; and compared with the example 1-2 with modified nano silicon dioxide, the compatibility among components in the cellulose carrier is reduced, and the pore structure is not uniform and compact enough, so that the adsorption and growth promotion effects on microalgae are not ideal enough, and the purification capability on sewage is obviously reduced. In conclusion, the microalgae culture method based on the modified cellulose carrier provided by the invention has good application prospects.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate technical solution, and this description is provided for clarity only, and those skilled in the art should consider the disclosure as a whole, and the technical solutions in the embodiments may be combined appropriately to form other embodiments that can be understood by those skilled in the art.
Claims (8)
1. The culture method of the microalgae based on the modified cellulose carrier is characterized by comprising the following steps of:
s1, carrying out hydroxylation treatment on nano silicon dioxide, and carrying out blending reaction on the nano silicon dioxide and alkyl carboxylic acid to obtain modified nano silicon dioxide;
s2, blending sodium hydroxide, urea and bamboo fibers in water and stirring to obtain a cellulose solution;
s3, placing the modified nano silicon dioxide into the cellulose solution, and uniformly stirring to obtain a mixed solution;
s4, adding the mixed solution into an acidic solution for curing to obtain a modified cellulose carrier;
s5, microalgae planting is carried out on the modified cellulose carrier.
2. The method for culturing microalgae based on modified cellulose carrier as claimed in claim 1, wherein the alkyl carboxylic acid is one or more selected from dodecanoic acid, tetradecanoic acid, hexadecanoic acid or octadecanoic acid.
3. The method for culturing microalgae based on modified cellulose carrier according to claim 1, wherein the mass ratio of the nano silicon dioxide to the bamboo fiber is 1:10-1:20.
4. The method for culturing microalgae based on modified cellulose carrier as claimed in claim 1, wherein in step S4, the acidic solution is a mixed solution of acetic acid and an organic solvent.
5. The method for culturing microalgae based on modified cellulose carrier as claimed in claim 1, wherein the microalgae is selected from one or more of chlorella, microcystis, chaetoceros, and rhodococcus.
6. The method of culturing microalgae based on modified cellulose carrier as claimed in claim 1, wherein in step S5, the microalgae is cultured to a stable growth stage before the microalgae colonize.
7. The method for culturing microalgae based on modified cellulose carrier according to claim 1, wherein in step S5, the temperature of the microalgae colonization is 20-25 ℃, and the illumination time is 3-6 h/day.
8. The method of culturing microalgae based on modified cellulose carrier as claimed in claim 1, characterized in that the size of the nanosilica is 10-100 nm.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103374313A (en) * | 2012-04-23 | 2013-10-30 | 上海杰事杰新材料(集团)股份有限公司 | Ethylene-vinyl acetate copolymer (EVA) adhesive film and preparation method thereof |
| CN106732477A (en) * | 2016-11-29 | 2017-05-31 | 陕西盛迈石油有限公司 | The preparation method of sodium alginate and cellulose aquagel ball |
| CN108192889A (en) * | 2018-01-11 | 2018-06-22 | 中南民族大学 | A kind of method of bacteria cellulose immobilized microalgae processing waste water |
| CN110975833A (en) * | 2019-12-18 | 2020-04-10 | 昆明理工大学 | Preparation method and application of silicon dioxide/cellulose composite porous material |
| CN114031170A (en) * | 2021-11-17 | 2022-02-11 | 华中科技大学 | Preparation method and application of microalgae biofilm based on modified cellulose |
| CN115043501A (en) * | 2022-07-30 | 2022-09-13 | 内蒙古美赢环保科技有限公司 | Domestic sewage treatment agent and preparation method thereof |
| CN116121233A (en) * | 2023-02-01 | 2023-05-16 | 深圳市百穗康实业有限公司 | Preparation method and application of biological film |
| CN116253426A (en) * | 2023-02-22 | 2023-06-13 | 深圳市百穗康实业有限公司 | Polymer biofilm packing and preparation method thereof |
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103374313A (en) * | 2012-04-23 | 2013-10-30 | 上海杰事杰新材料(集团)股份有限公司 | Ethylene-vinyl acetate copolymer (EVA) adhesive film and preparation method thereof |
| CN106732477A (en) * | 2016-11-29 | 2017-05-31 | 陕西盛迈石油有限公司 | The preparation method of sodium alginate and cellulose aquagel ball |
| CN108192889A (en) * | 2018-01-11 | 2018-06-22 | 中南民族大学 | A kind of method of bacteria cellulose immobilized microalgae processing waste water |
| CN110975833A (en) * | 2019-12-18 | 2020-04-10 | 昆明理工大学 | Preparation method and application of silicon dioxide/cellulose composite porous material |
| CN114031170A (en) * | 2021-11-17 | 2022-02-11 | 华中科技大学 | Preparation method and application of microalgae biofilm based on modified cellulose |
| CN115043501A (en) * | 2022-07-30 | 2022-09-13 | 内蒙古美赢环保科技有限公司 | Domestic sewage treatment agent and preparation method thereof |
| CN116121233A (en) * | 2023-02-01 | 2023-05-16 | 深圳市百穗康实业有限公司 | Preparation method and application of biological film |
| CN116253426A (en) * | 2023-02-22 | 2023-06-13 | 深圳市百穗康实业有限公司 | Polymer biofilm packing and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| JIA XIN YAP等: "Sustainable cultivation of Navicula incerta using cellulose-based scaffold incorporated with nanoparticles in air-liquid interface cultivation system", 《CHEMOSPHERE》, vol. 273, pages 1 - 10 * |
| 张茜: "木质纤维素基藻类生物膜废水处理实验研究", 《中国优秀硕士学位论文全文数据库》, 15 June 2019 (2019-06-15) * |
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