CN215050204U - Little algae group banks up device with earth - Google Patents

Abstract

The utility model discloses a microalgae tissue culture device, which comprises a microalgae culture container, a lighting device, a fungus culture device, an oxygen dissolving device and a water culture device, wherein the fungus culture device is arranged at the bottom of an inner cavity of the microalgae culture container; fungus class culture apparatus is including the fungus bed layer and being the fungus bed fixed bed, and dissolved oxygen device includes aeration pipe and air compressor machine, and the water culture apparatus is including the water pump and the floater that have filter material, and the water pump is placed on the fungus bed fixed bed, is connected with the floater through the pipeline. The utility model discloses utilize fungus algae intergrowth principle to establish fungus algae intergrowth system, be favorable to little algae to be cultivateed, improve the quality of water condition, simple structure, the scale production of being convenient for.

Description

Little algae group banks up device with earth

Technical Field

The utility model belongs to the technical field of it is biological, concretely relates to little algae group banks up device with earth.

Background

Microalgae such as chlorella, haematococcus pluvialis, spirulina and the like absorb carbon dioxide through photosynthesis to produce useful components such as vitamins, amino acids, pigments, proteins, polysaccharides, fatty acids and the like, absorb harmful substances such as nitrite in water and can be used as feed for cultivation, and the microalgae are used as means for treating carbon dioxide which is one of causes of global warming. EM bacteria are viable bacteria preparations consisting of various beneficial microorganisms, and are widely used as water quality purification modifiers and feed additives for aquaculture to purify water quality, enhance immunity of cultured objects and improve yield and benefit. The photosynthesis of algae releases oxygen for the metabolism of aerobic heterotrophic microorganisms, and the aerobic heterotrophic microorganisms carry out oxidative decomposition on organic pollutants to generate CO2 and inorganic nitrogen and phosphorus compounds which provide carbon sources and nutrients required by the photosynthesis for the algae. At present, a bacterial-algae symbiotic system established on the basis of a theory of algae-bacteria symbiosis has shown great advantages in urban sewage treatment, but the research on the aspect of aquaculture wastewater purification is very little.

The problems that a culture solution cannot be fully stirred, illumination is insufficient, algae are easily polluted and the like generally exist in the conventional culture mode in production application, so that the production capacity is low, the stability is poor and the risk resistance is weak. Meanwhile, illumination is a necessary condition for growth of microalgae plants, and is a complex ecological factor mainly comprising factors in the aspects of illumination period, illumination intensity, spectrum composition and the like.

Therefore, there is a need to improve current microalgae cultivation equipment.

SUMMERY OF THE UTILITY MODEL

The to-be-solved technical problem of the utility model is to provide a little algae group banks up device with earth of simple structure, convenient to use for through fungus algae intergrowth technique, cooperation LED illumination establishes little algae group and banks up system with earth, provides technical support for aquaculture quality of water regulation and control.

In order to solve the technical problem, the utility model provides a microalgae tissue culture device, which comprises a microalgae culture container, a lighting device, a fungus culture device, an oxygen dissolving device and a water culture device, wherein the fungus culture device is arranged at the bottom of an inner cavity of the microalgae culture container;

the fungus culture device comprises a fungus bed layer and a fungus bed fixing layer, the fungus bed layer is paved at the bottom of the algae culture container, and the size and the shape of the fungus bed fixing layer are matched with the size and the shape of the bottom of the algae culture container and are placed on the fungus bed layer;

the oxygen dissolving device comprises an aeration pipe and an air compressor, wherein the aeration pipe is fixedly arranged on the fixed layer of the bacterial bed through a bayonet and is arranged into an inner ring shape and an outer ring shape, the air compressor is arranged outside the algae culture container, and the aeration pipe is connected with an air outlet of the air compressor through a pipeline;

the water culture device comprises a water pump with filter materials and a floating ball, wherein the water pump is placed on the bacteria bed fixing layer and is connected with the floating ball through a pipeline.

As an improvement of the microalgae tissue culture device of the utility model:

the light device comprises a full-spectrum LED lamp and a light support, the full-spectrum LED lamp is fixedly connected with the light support, and the irradiation direction of the full-spectrum LED lamp is opposite to the inner cavity of the algae cultivation container.

As the utility model discloses a little algae group banks up further improvement of device with earth:

the algae culturing container is a container with an opening at the top, and sealed at the periphery and the bottom.

As the utility model discloses a little algae group banks up further improvement of device with earth:

the fungus bed layer comprises a ceramsite layer and a biochemical cotton layer, and the specific gravity of the fungus bed fixing layer is greater than that of water.

The beneficial effects of the utility model are mainly embodied in that:

1. the utility model utilizes the principle of fungus-algae symbiosis to establish a fungus-algae symbiosis system, which is beneficial to the culture of microalgae, improves the water quality condition, has simple structure and is convenient for large-scale production;

2. the utility model is matched with the full spectrum LED illumination to supplement an extra light source, which is beneficial to algae cultivation;

3. the utility model adopts the water culture device to promote the exchange of upper and lower water bodies and increase the fault tolerance of the water environment;

4. the utility model discloses when cultivateing little algae, cultivate the dominant bacterial through fungus class culture apparatus synchronization, carry out quality of water optimization.

Drawings

The following describes the present invention in further detail with reference to the accompanying drawings.

Fig. 1 is a schematic structural diagram of a microalgae tissue culture device of the utility model.

Detailed Description

The invention will be further described with reference to specific examples, but the scope of protection of the invention is not limited thereto:

example 1, a microalgae tissue culture apparatus, as shown in fig. 1, includes a culture vessel, a fungus culture apparatus, a lighting apparatus, an oxygen dissolving apparatus, and a water culture apparatus.

The algae culturing container is a container with an opening at the top, and sealed at the periphery and the bottom, for example, can be barrel-shaped, box-shaped and the like, and is used for loading a culturing water body required by culturing microalgae, so as to achieve the purpose of industrial production, the effective volume in the algae culturing container is at least 7 cubic meters, the culturing water body is loaded in the algae culturing container, and the depth of the culturing water body is at least 1 meter; the bottom of the inner cavity of the algae culture container is provided with a fungus culture device, so that the effects of water quality filtration and bacteria culture are achieved, the fungus culture device comprises a fungus bed layer 101 and a fungus bed fixing layer 102, the fungus bed fixing layer 102 is a 5 cm-thick pine board, the size and the shape of the pine board are matched with those of the bottom of the algae culture container, the pine board is not easy to rot and can sink to the bottom, the pine board is placed on the fungus bed layer 101 and used for preventing the fungus bed layer 101 from floating upwards, the fungus bed layer 101 comprises a 10 cm-thick ceramic grain layer and a 5 cm-thick biochemical cotton layer, the biochemical cotton is placed on the ceramic grain, the biochemical cotton and the ceramic grain are both paved on the whole algae culture container, the ceramic grain can parasitize more bacteria due to larger gaps, the biochemical cotton has high density compared with filter cotton and is not easy to damage, and has the biochemical filtration effect and has enough space for parasitizing bacteria;

the culture water body loaded in the algae culture container comprises purified water and a culture medium, wherein the formula of the culture medium comprises 1.5g/L of sodium nitrate, 0.08g/L of magnesium sulfate, 0.03g/L of calcium chloride, 0.05g/L of monopotassium phosphate, 18g/m3 of urea concentrated solution and 1ml/m3 of biological essential fertilizer;

the lighting device is arranged above the top opening of the algae cultivation container and comprises at least one full-spectrum LED lamp 201 and a lighting support 202, the full-spectrum LED lamp 201 is fixedly connected with the lighting support 202, the full-spectrum LED lamp 201 is 30cm away from the water surface of the water body for cultivation, so that light of the full-spectrum LED lamp 201 can irradiate and fill the inside of the algae cultivation container, the light intensity of the full-spectrum LED lamp 201 is 3500-4500 Lx, the spectrum is full spectrum, and the power is 50 w;

the dissolved oxygen device comprises an aerator pipe 301 and an air compressor 302, the aerator pipe 301 can adopt a microporous aerator or a hose type aerator, the aerator pipe 301 is fixedly arranged on a pinewood plate at the bottom of the algae culture container into the shape of inner and outer 2 circular rings through a bayonet, the inner circular ring of the aerator pipe 301 is arranged at the central position of the algae culture container, the diameter is 1m, the outer circular ring of the aerator pipe 301 is arranged along the wall of the algae culture container, the diameter of the outer circular ring is as large as possible, so that the whole water body for culture can be filled after the gas comes out of the aerator pipe 301, the air compressor 302 is arranged outside the algae culture container, the aerator pipe 301 is connected with the air outlet of the air compressor 302 through a pipeline, and the aeration amount is 500L/min;

the water culture device is arranged in the algae culture container and comprises a water pump 401 with a filter material, the water pump 401 is placed on a pinewood plate at the bottom of the algae culture container and is connected with a floating ball 402 through a pipeline, the floating ball 402 is arranged on the upper layer of a water body for culture, when the water pump 401 works, the water body for culture at the bottom of the algae culture container is sucked, conveyed to the floating ball 402 and output, and is exchanged from the upper layer water body and the lower layer water body which can push the water body for culture, so that the function of controlling bacteria is achieved.

The method for culturing microalgae by using the utility model is as follows:

1. water preparation

A 10 cm-thick ceramsite fungus bed layer, a 5 cm-thick biochemical cotton layer and a 5 cm-thick pine board layer are sequentially arranged at the bottom of the inner cavity of the algae culture container from bottom to top;

injecting purified water into the algae culture container, wherein the water depth is about 1m, the effective water body is 7 cubic meters, and opening an air compressor 302 to aerate the water body through an aeration pipe 301; in the microalgae culture period, the oxygen dissolving device is required to be always in a working state to aerate the water body;

2. preparation of the Medium

Aerating for 24h, adding a culture medium (the formula of the culture medium is 1.5g/L of sodium nitrate, 0.08g/L of magnesium sulfate, 0.03g/L of calcium chloride, 0.05g/L of monopotassium phosphate, 18g/m3 of urea concentrated solution and 1ml/m3 of biological fertilizer), preparing the culture medium according to a certain proportion, adding the prepared culture medium into a water body, and uniformly stirring;

in the microalgae culture period, only the culture medium needs to be supplemented for one time;

3. inoculation of bacteria and algae

The EM bacteria generally comprise photosynthetic bacteria, saccharomycetes, lactic acid bacteria and other beneficial bacteria, can be used for disease control and water quality improvement of algae culture, and the microalgae to be cultured mainly comprises 60% of chlorella, 20% of haematococcus pluvialis and 20% of oocyst algae;

inoculating EM bacteria (0.03-0.05 ‰) and algae (0.002-0.005 ‰) into culture water, and stirring;

4. cultivation of algal species

Turning on a full-spectrum LED lamp 201 to supplement light to the culture water body, turning on a water pump 401 to push the exchange of the upper layer and the lower layer of the culture water body, and during the culture of algae seeds, keeping a lighting device, an oxygen dissolving device and a water culture device in working states all the time, thereby ensuring the growth environment of bacteria and algae;

the period of the culture of the algae is 7 days, and the content of the microalgae can reach 1 multiplied by 10^ 9/ml.

5. After the culture is finished, the culture water body with the microalgae is placed into an external storage device, then the oxygen dissolving device, the water culture device and the microalgae culture container are thoroughly cleaned, a ceramsite layer and a biochemical cotton layer in the fungus culture device are replaced, and the next round of microalgae culture is prepared.

Experiment 1:

set up little algae group and cultivate the device according to example 1, correspondingly, adopt ordinary algae bucket of cultivateing to cultivate algae as the contrast, specifically set up as follows:

aiming at the microalgae tissue culture device in the embodiment 1, the microalgae is cultured according to the using method in the embodiment 1, and correspondingly, purified water, a culture medium and bacterial algae seeds are added into a common algae culturing barrel, stirred uniformly and kept stand; after 7 days, the counting mode of a microscope is used for measuring the microalgae content in the microalgae tissue culture device of the embodiment 1 to reach 1 x 10^ 9/ml, and the microalgae content in the common microalgae culture barrel is 1 x 10^ 6/ml, thereby verifying the effectiveness of the utility model.

Finally, it is also noted that the above-mentioned list is only a few specific embodiments of the present invention. Obviously, the present invention is not limited to the above embodiments, and many modifications are possible. All modifications which can be derived or suggested by a person skilled in the art from the disclosure of the invention should be considered as within the scope of the invention.

Claims (4)

1. A microalgae tissue culture device is characterized by comprising an algae culture container, a lighting device, a fungus culture device, an oxygen dissolving device and a water culture device, wherein the fungus culture device is arranged at the bottom of an inner cavity of the algae culture container;

the fungus culture device comprises a fungus bed layer (101) and a fungus bed fixing layer (102), wherein the fungus bed layer (101) is paved at the bottom of an algae culture container, and the size and the shape of the fungus bed fixing layer (102) are matched with those of the bottom of the algae culture container and are placed on the fungus bed layer (101);

the oxygen dissolving device comprises an aeration pipe (301) and an air compressor (302), wherein the aeration pipe (301) is fixedly arranged on the bacteria bed fixing layer (102) through a bayonet and is arranged into an inner ring shape and an outer ring shape, the air compressor (302) is arranged outside the algae culture container, and the aeration pipe (301) is connected with an air outlet of the air compressor (302) through a pipeline;

the water culture device comprises a water pump (401) with filter materials and a floating ball (402), wherein the water pump (401) is placed on the fungus bed fixing layer (102) and is connected with the floating ball (402) through a pipeline.

2. The microalgae tissue culture device of claim 1, wherein:

the lighting device comprises a full-spectrum LED lamp (201) and a lighting support (202), the full-spectrum LED lamp (201) is fixedly connected with the lighting support (202), and the irradiation direction of the full-spectrum LED lamp (201) is opposite to the inner cavity of the algae cultivation container.

3. The microalgae tissue culture device of claim 2, wherein:

the algae culturing container is a container with an opening at the top, and sealed at the periphery and the bottom.

4. The microalgae tissue culture device of claim 3, characterized in that:

the fungus bed layer (101) comprises a ceramsite layer and a biochemical cotton layer, and the specific gravity of the fungus bed fixing layer (102) is larger than that of water.

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