CN107736283A - The energy-saving and emission-reduction method for culturing seedlings of blue full dress sea urchin - Google Patents

The energy-saving and emission-reduction method for culturing seedlings of blue full dress sea urchin Download PDF

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Publication number
CN107736283A
CN107736283A CN201710865106.1A CN201710865106A CN107736283A CN 107736283 A CN107736283 A CN 107736283A CN 201710865106 A CN201710865106 A CN 201710865106A CN 107736283 A CN107736283 A CN 107736283A
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young
courage
cells
water
sea urchin
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CN107736283B (en
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张伟杰
王宝锋
黄堯
胡方圆
郑棋元
常亚青
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Dalian Ocean University
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Dalian Ocean University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/30Culture of aquatic animals of sponges, sea urchins or sea cucumbers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/10Culture of aquatic animals of fish
    • A01K61/17Hatching, e.g. incubators
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/003Aquaria; Terraria
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/04Arrangements for treating water specially adapted to receptacles for live fish
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/06Arrangements for heating or lighting in, or attached to, receptacles for live fish
    • A01K63/065Heating or cooling devices
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention discloses a kind of method for culturing seedlings of blue full dress sea urchin, compared with traditional sea urchin method for culturing seedlings, can be under extremely low energy resource consumption and breeding wastewater conditions of discharge, cultivate a large amount of blue full dress sea urchin seeds, to reach the pressure that fishes for reducing natural resources, solves the purpose for the problems such as cost of transportation is high and survival rate is low.Breeding environment has been placed in warmhouse booth by it first, the water temperature of breeding water body is allowed to be maintained in of a relatively high scope in the presence of warmhouse booth, nursery is carried out without using artificial heating seawater, on the premise of cost-effective, boiler avoided and is polluted for environment;Launch Chaetoceros muelleri and seawater photosynthetic bacteria using the illumination cultivation young in the range of 1500 ~ 2500 lx in cultivating process simultaneously, the purpose to purify water can be reached, avoid it is a large amount of, frequently change water and caused Cost Problems.

Description

The energy-saving and emission-reduction method for culturing seedlings of blue full dress sea urchin
Technical field
The present invention relates to sea urchin to breed field, particularly a kind of energy-saving and emission-reduction method for culturing seedlings of blue full dress sea urchin.
Background technology
Blue full dress sea urchin(Mespilia globulus)It is under the jurisdiction of Echinodermata, Echinoidea, Centrechinoida, carves rib sea urchin Section, English name Blue Tuxedo Urchin.The Indian Ocean, the Pacific Ocean and China South Sea are distributed in until South Japan sea Domain.Blue full dress sea urchin shell has five loose bluenesss or black texture, and thorn is red, brown or black, and colour match is very Beauty, great ornamental value, and raising can help to gnaw liverwort algae in aquarium, thus cultivated extensively in the seawater Shui nationality In cylinder.
Blue full dress sea urchin market demand is very big, and the blue full dress sea urchin of current domestic Shui nationality market sale is all nature fishing In Tropical Ocean Areas such as the Indian Ocean, and air transport, to destination, the high cost caught and transported causes the price of a blue full dress sea urchin Up to dozens of yuan or even more than hundred yuan.And the transport of long range reduces its survival rate, larger warp is brought to consumer Ji loss.The maximally effective method that solves the above problems is exactly to carry out the artificial breeding of blue full dress sea urchin at home.However, at present also The not relevant report on blue full dress sea urchin artificial breeding, existing cold aqueous sea urchin(Such as Strongylocentrotus intermedius)Seedling-raising technique And tropical sea urchin(Such as Anthocidaris crassispina)Although seedling-raising technique can be used as reference, due to water temperature, bait needed for variety classes sea urchin The each side condition differences such as material are very big, find that blue full dress sea urchin nursery is continued to use other species prior arts and can not obtained into practice Work(.In addition, blue full dress sea urchin is tropical species, high temperature seawater is needed during seed rearing(25—30℃)If opened in the north Open up artificial breeding and use the breeding facility and water changing method of other sea urchins use(Daily 1 ~ 2 time, every time 1/3 ~ 1/2), then must Mass energy need be consumed with the seawater that heats up, energy resource consumption and cost in seedling raising process so can not only be significantly increased, can also Because burning boiler or waste discharge etc. pollute to environment.
The content of the invention
The present invention be in order to solve the above-mentioned deficiency present in prior art, propose it is a kind of can in extremely low energy resource consumption and Under breeding wastewater conditions of discharge, a large amount of blue full dress sea urchin seeds are cultivated, to reach the pressure that fishes for reducing natural resources, are solved The method for culturing seedlings for the problems such as cost of transportation is high and survival rate is low.
The present invention technical solution be:A kind of energy-saving and emission-reduction method for culturing seedlings of blue full dress sea urchin, it is characterised in that:Institute The method stated is selected by close courage, obtains embryonated egg, the cultivation of floating larva, the metamorphosis of larva and attachment, the cultivation of young courage, young courage Stripping and intermediate rearing step composition, and above-mentioned steps are carried out in warmhouse booth, and the ceiling of warmhouse booth has removable Dynamic sunshade net, while the side of warmhouse booth is provided with the ventilating opening that can be turned on and off, in the operating process of this method, Temperature range in warmhouse booth is 25-40 DEG C, and the water temperature range in warmhouse booth in larval culture pond is 25-30 DEG C,
Described close courage selection step for:From shell footpath in more than 3cm, vigor is good and the ripe sea urchin of surface N/D is as parent Courage,
Described acquisition embryonated egg step is:Induction induces close courage spawning row's essence, and smart ovum is mixed and obtains embryonated egg, will be fertilized Ovum, which is put into 25 ~ 28 DEG C of seawater, hatches, and hatching density is 20-30/mL, be fertilized 1h when wash ovum once, be fertilized 2h when wash again Ovum once, and select it is excellent,
The incubation step of described floating larva is:By select it is excellent after the young be put into culture pond and cultivated, cultivating density is 0.5 ~ 0.8/mL, start to feed bait after the prism phase, before terminating to the four wrist young, feed daily Chaetoceros muelleri 5000 ~ 10000 cells/mL, 2000 cells of seawater photosynthetic bacteria/mL, six wrist young periods feed daily Chaetoceros muelleri 10000 ~ 20 20000 cells/mL, 2000 cells of seawater photosynthetic bacteria/mL, brachionus plicatilis/mL, eight wrist young periods feed daily 40 20000 ~ 30000 cells of Chaetoceros muelleri/mL, 2000 cells of seawater photosynthetic bacteria/mL, brachionus plicatilis/mL, every Adding water after inhaling bottom with siphon pipe within 2 days, water absorption is 1/10th of full water amount,
The metamorphosis of described larva and attachment steps are:It is compound to there is vestibular to eight wrist larva later stages in stomach side for larvae development Body, when 5-10% individual pipe podocytic process goes out vestibular cavity wall, immigration after all larvae collections is placed with to the culture pond of corrugated plating In, cultivation density is 0.2 ~ 0.4/cm2, it is inoculated with benthic diatom on described corrugated plating in advance, illumination in cultivating process 1500 ~ 2500 lx, 25 ~ 30 DEG C of water temperature, 5 ~ 7 days full doses change water 1 time, and the clown fish 5 ~ 8 of 2 ~ 3 cm bodies length is launched in cultivating process Only/m3, corrugated plating is horizontal positioned when larva is launched, and corrugated plating is overturn into 180 ° of placements after 1-2 days, after 5 days that corrugated plating is vertical Place, after corrugated plating is placed vertically, start to feed benthic diatom into culture pond, feed daily once, every time 600 cells/ ML, continuously feed 10 days,
In the incubation step of the young courage, nutritive salt is added into culture pond, the amount of the nutritive salt of addition is daily N: P: Si: Fe =2: 0.1: 0.1: 0.01ppm, intensity of illumination is controlled in below 3000Lux, natural photoperiod,
The stripping of described young courage and intermediate rearing step are:After young courage grows 1 month on corrugated plating, shelled with banister brush From intermediate rearing is carried out to being placed with black corrugated plating and water tank with temperature control device, sea lettuce is fed into water tank, 2~3 days Feed once, add seawater photosynthetic bacteria according to 2000 cells/mL amount daily, when being less than below 0.5cm in young courage shell footpath, training It is 5000/m to educate density2, when being more than 0.5cm in young courage shell footpath, cultivation density is 2000/m2, during intermediate rearing, water Temperature control system is at 26~30 DEG C, salinity 29~30 ‰, pH7.5~8.0.
The present invention compared with the existing technology, has the following advantages that:
The method for culturing seedlings of blue full dress sea urchin disclosed in this invention, can be in extremely low energy compared with traditional sea urchin method for culturing seedlings Source is consumed and under breeding wastewater conditions of discharge, cultivates a large amount of blue full dress sea urchin seeds, and fishing for natural resources is reduced to reach Pressure, solves the purpose for the problems such as cost of transportation is high and survival rate is low.Breeding environment has been placed in warmhouse booth by it first, Allow the water temperature of breeding water body to be maintained in of a relatively high scope in the presence of warmhouse booth, enter without using artificial heating seawater Row nursery, on the premise of cost-effective, avoid boiler and polluted for environment;1500 are used in cultivating process simultaneously The illumination cultivation young in the range of ~ 2500 lx, Chaetoceros muelleri and seawater photosynthetic bacteria are launched, can reach what is purified water Purpose, avoid it is a large amount of, frequently change water and caused Cost Problems;And to cultivation in the metamorphosis, attaching process also in larva Clown fish is launched in pond, is evenly distributed using the travelling nutritive salt for allowing application of fish in water body, while clown fish can ingest food Protozoan on benthic diatom plate, and its caused excrement is alternatively arranged as the Fertilizer application of benthic diatom;In summary, may be used It is very wide particularly suitable for popularization and application in the art, its market prospects to say that this method possesses a variety of advantages.
Embodiment
The embodiment of the present invention is described below.
A kind of energy-saving and emission-reduction method for culturing seedlings of blue full dress sea urchin, by the selection of close courage, the training of acquisition embryonated egg, floating larva Educate, the metamorphosis of larva and attachment, the cultivation of young courage, the stripping of young courage and intermediate rearing step form, and above-mentioned steps are in temperature Carried out in the greenhouse of room, the ceiling of warmhouse booth has moveable sunshade net, while the side of warmhouse booth is provided with and can opened Or the ventilating opening closed, in the operating process of this method, the temperature range in warmhouse booth is 25-40 DEG C, in warmhouse booth Water temperature range in larval culture pond is 25-30 DEG C,
Described close courage selection step for:From shell footpath in more than 3cm, vigor is good and the ripe sea urchin of surface N/D is as parent Courage,
Described acquisition embryonated egg step is:Induction induces close courage spawning row's essence, and smart ovum is mixed and obtains embryonated egg, will be fertilized Ovum, which is put into 25 ~ 28 DEG C of seawater, hatches, and hatching density is 20-30/mL, be fertilized 1h when wash ovum once, be fertilized 2h when wash again Ovum once, and select it is excellent,
The incubation step of described floating larva is:By select it is excellent after the young be put into culture pond and cultivated, cultivating density is 0.5 ~ 0.8/mL, start to feed bait after the prism phase, before terminating to the four wrist young, feed daily Chaetoceros muelleri 5000 ~ 10000 cells/mL, 2000 cells of seawater photosynthetic bacteria/mL, six wrist young periods feed daily Chaetoceros muelleri 10000 ~ 20 20000 cells/mL, 2000 cells of seawater photosynthetic bacteria/mL, brachionus plicatilis/mL, eight wrist young periods feed daily 40 20000 ~ 30000 cells of Chaetoceros muelleri/mL, 2000 cells of seawater photosynthetic bacteria/mL, brachionus plicatilis/mL, every Adding water after inhaling bottom with siphon pipe within 2 days, water absorption is 1/10th of full water amount,
The metamorphosis of described larva and attachment steps are:It is compound to there is vestibular to eight wrist larva later stages in stomach side for larvae development Body, when 5-10% individual pipe podocytic process goes out vestibular cavity wall, immigration after all larvae collections is placed with to the culture pond of corrugated plating In, cultivation density is 0.2 ~ 0.4/cm2, it is inoculated with benthic diatom on described corrugated plating in advance, illumination in cultivating process 1500 ~ 2500 lx, 25 ~ 30 DEG C of water temperature, 5 ~ 7 days full doses change water 1 time, and the clown fish 5 ~ 8 of 2 ~ 3 cm bodies length is launched in cultivating process Only/m3, corrugated plating is horizontal positioned when larva is launched, and corrugated plating is overturn into 180 ° of placements after 1-2 days, after 5 days that corrugated plating is vertical Place, after corrugated plating is placed vertically, start to feed benthic diatom into culture pond, feed daily once, every time 600 cells/ ML, continuously feed 10 days,
In the incubation step of the young courage, nutritive salt is added into culture pond, the amount of the nutritive salt of addition is daily N: P: Si: Fe =2: 0.1: 0.1: 0.01ppm, intensity of illumination is controlled in below 3000Lux, natural photoperiod,
The stripping of described young courage and intermediate rearing step are:After young courage grows 1 month on corrugated plating, shelled with banister brush From intermediate rearing is carried out to being placed with black corrugated plating and water tank with temperature control device, sea lettuce is fed into water tank, 2~3 days Feed once, add seawater photosynthetic bacteria according to 2000 cells/mL amount daily, when being less than below 0.5cm in young courage shell footpath, training It is 5000/m to educate density2, when being more than 0.5cm in young courage shell footpath, cultivation density is 2000/m2, during intermediate rearing, water Temperature control system is at 26~30 DEG C, salinity 29~30 ‰, pH7.5~8.0.
The nursery for carrying out blue full dress sea urchin is such as used conventional methods, due to needing largely to change water, therefore the energy can be increased Consumption and pollution environment, in addition, the pluteus postoral arm and postero-dorsal arm of blue full dress sea urchin are roomy, change water or the operation of falling pond compared with it His sea urchin is easier by mechanical injuries, dead quickly if damage, causes nursery to fail.For this problem, we Method launches Chaetoceros muelleri and seawater photosynthetic bacteria using the illumination cultivation young in the range of 1500 ~ 2500 lx.One side angle Hair algae and photosynthetic bacteria can be used as bait, and on the other hand under this illumination, Chaetoceros and photosynthetic bacteria can be using excrement and residual Inorganic and organic nutrition salt caused by bait degraded carries out photosynthesis, reaches the purpose to purify water.It was verified that above-mentioned Under the conditions of, water can not be changed for a long time, or suction bottom only need to be carried out with siphon pipe daily, and water absorption need to only reach 1/10th or so, Every water quality index in nursery pond can maintain normal level.
In addition in the cultivating process of floating larva, when blue full dress echinopluteus is developed to the six wrist young, young oesophagus increases Greatly to 20 ~ 30um, therefore brachionus plicatilis can be added in bait in this step, improve the young and ingest and effect and ensure it Nutritional need;
In the overall process of this method, using the opening and closing of greenhouse internal sunshade net and ventilating opening, greenhouse temperature is maintained at 25-40 DEG C, Avoid using artificial heating seawater seed rearing, saved cost;Burning boiler is it also avoid to pollute environment;
In the metamorphosis of larva and attachment steps, need to be inoculated with benthic diatom in advance on corrugated plating, its condition of culture and conventional strip Part difference mainly has:1500 ~ 2500lx of illumination, 25 ~ 30 DEG C of water temperature, not flowing water, but 5 ~ 7 days full doses change water 1 time, cultivate 5 ~ 8/m of clown fish of 2 ~ 3 cm bodies length is launched in journey3.The purpose one for launching clown fish is to make the nutrition of application by fish swimming Salt is evenly distributed in water body, the protozoan on benthic diatom plate second, clown fish can ingest, third, excrement caused by clown fish Can be as the fertilizer of benthic diatom, the good and fast growth of benthic diatom can be made by launching clown fish;
After being placed vertically for the corrugated plating collected seedling, start to feed other into water not being thrown on Miao Chi corrugated plating and scrub The benthic diatom to get off, feed once, every time 500 ~ 1000 cells/mL, continuously feed 10 days daily, this operation can make to be attached to The young sea urchin of bottom of pond drops to the young sea urchin of bottom of pond from vertical corrugated plating and feeds into the benthic diatom conduct for feeding and sinking to the bottom Bait, improve the survival rate of young sea urchin.

Claims (1)

  1. A kind of 1. energy-saving and emission-reduction method for culturing seedlings of blue full dress sea urchin, it is characterised in that:Described method selects by close courage, obtain by Smart ovum, the cultivation of floating larva, the metamorphosis of larva and attachment, the cultivation of young courage, the stripping of young courage and intermediate rearing step composition, And above-mentioned steps are carried out in warmhouse booth, the ceiling of warmhouse booth has a moveable sunshade net, while warmhouse booth Side is provided with the ventilating opening that can be turned on and off, and in the operating process of this method, the temperature range in warmhouse booth is 25- 40 DEG C, the water temperature range in warmhouse booth in larval culture pond is 25-30 DEG C,
    Described close courage selection step for:From shell footpath in more than 3cm, vigor is good and the ripe sea urchin of surface N/D is as parent Courage,
    Described acquisition embryonated egg step is:Induction induces close courage spawning row's essence, and smart ovum is mixed and obtains embryonated egg, will be fertilized Ovum, which is put into 25 ~ 28 DEG C of seawater, hatches, and hatching density is 20-30/mL, be fertilized 1h when wash ovum once, be fertilized 2h when wash again Ovum once, and select it is excellent,
    The incubation step of described floating larva is:By select it is excellent after the young be put into culture pond and cultivated, cultivating density is 0.5 ~ 0.8/mL, start to feed bait after the prism phase, before terminating to the four wrist young, feed daily Chaetoceros muelleri 5000 ~ 10000 cells/mL, 2000 cells of seawater photosynthetic bacteria/mL, six wrist young periods feed daily Chaetoceros muelleri 10000 ~ 20 20000 cells/mL, 2000 cells of seawater photosynthetic bacteria/mL, brachionus plicatilis/mL, eight wrist young periods feed daily 40 20000 ~ 30000 cells of Chaetoceros muelleri/mL, 2000 cells of seawater photosynthetic bacteria/mL, brachionus plicatilis/mL, every Adding water after inhaling bottom with siphon pipe within 2 days, water absorption is 1/10th of full water amount,
    The metamorphosis of described larva and attachment steps are:It is compound to there is vestibular to eight wrist larva later stages in stomach side for larvae development Body, when 5-10% individual pipe podocytic process goes out vestibular cavity wall, immigration after all larvae collections is placed with to the culture pond of corrugated plating In, cultivation density is 0.2 ~ 0.4/cm2, it is inoculated with benthic diatom on described corrugated plating in advance, illumination in cultivating process 1500 ~ 2500 lx, 25 ~ 30 DEG C of water temperature, 5 ~ 7 days full doses change water 1 time, and the clown fish 5 ~ 8 of 2 ~ 3 cm bodies length is launched in cultivating process Only/m3, corrugated plating is horizontal positioned when larva is launched, and corrugated plating is overturn into 180 ° of placements after 1-2 days, after 5 days that corrugated plating is vertical Place, after corrugated plating is placed vertically, start to feed benthic diatom into culture pond, feed daily once, every time 600 cells/ ML, continuously feed 10 days,
    In the incubation step of the young courage, nutritive salt is added into culture pond, the amount of the nutritive salt of addition is daily N: P: Si: Fe =2: 0.1: 0.1: 0.01ppm, intensity of illumination is controlled in below 3000Lux, natural photoperiod,
    The stripping of described young courage and intermediate rearing step are:After young courage grows 1 month on corrugated plating, shelled with banister brush From intermediate rearing is carried out to being placed with black corrugated plating and water tank with temperature control device, sea lettuce is fed into water tank, 2~3 days Feed once, add seawater photosynthetic bacteria according to 2000 cells/mL amount daily, when being less than below 0.5cm in young courage shell footpath, training It is 5000/m to educate density2, when being more than 0.5cm in young courage shell footpath, cultivation density is 2000/m2, during intermediate rearing, water Temperature control system is at 26~30 DEG C, salinity 29~30 ‰, pH7.5~8.0.
CN201710865106.1A 2017-09-22 2017-09-22 Energy-saving emission-reduction seedling raising method for blue robe sea urchins Active CN107736283B (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111296336A (en) * 2020-04-09 2020-06-19 海南蓝泰邦生物技术有限公司 Method for cultivating sea urchin fries of white spine three rows
CN112471014A (en) * 2020-11-25 2021-03-12 大连海洋大学 Method for breeding sea urchin seedlings through medium ball of high-density attached plates

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WO2008074084A1 (en) * 2006-12-20 2008-06-26 Macquarie University Production of sea urchin roe
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CN105766707A (en) * 2016-02-01 2016-07-20 大连海洋大学 Cultivation method for high temperature resistance sea urchins

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Publication number Priority date Publication date Assignee Title
WO2008074084A1 (en) * 2006-12-20 2008-06-26 Macquarie University Production of sea urchin roe
CN101940171A (en) * 2010-07-26 2011-01-12 大连太平洋海珍品有限公司 Breeding method of glyptocidaris crenularis offspring seed
CN105766707A (en) * 2016-02-01 2016-07-20 大连海洋大学 Cultivation method for high temperature resistance sea urchins

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Title
常亚青: "中间球海胆"大金"", 《中国水产》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111296336A (en) * 2020-04-09 2020-06-19 海南蓝泰邦生物技术有限公司 Method for cultivating sea urchin fries of white spine three rows
CN112471014A (en) * 2020-11-25 2021-03-12 大连海洋大学 Method for breeding sea urchin seedlings through medium ball of high-density attached plates

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