CN101940171A - Breeding method of glyptocidaris crenularis offspring seed - Google Patents

Breeding method of glyptocidaris crenularis offspring seed Download PDF

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Publication number
CN101940171A
CN101940171A CN2010102355838A CN201010235583A CN101940171A CN 101940171 A CN101940171 A CN 101940171A CN 2010102355838 A CN2010102355838 A CN 2010102355838A CN 201010235583 A CN201010235583 A CN 201010235583A CN 101940171 A CN101940171 A CN 101940171A
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China
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courage
young
water
choline
hatching
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CN2010102355838A
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刘明泰
李丹
王秀丽
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DALIAN PACIFIC SEAFOOD Co Ltd
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DALIAN PACIFIC SEAFOOD Co Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention discloses a breeding method of a glyptocidaris crenularis offspring seed, comprising the following steps of: cultivating chaetoceros in a high temperature season, centrifugally concentrating and then storing at low temperature; beginning to cultivate benthic diatoms 10-20 days before collecting seedling of glyptocidaris crenularis, and selecting an individual with a shell diameter of at least 8 cm, health and no damage and favorable gonadal development at 2-3 ages as an affinity choline; inducing the affinity choline to lay eggs by using a KCl solution stimulus method, artificially inseminating, then hatching the germ cells in ponds according to the density of 3-5 per ml, micro-aerating in the hatching period and timing-stirring, wherein the hatching water temperature is 15-18 DEG C; selecting healthy larvae at the upper layer, breeding according to the density of 0.4-0.6 per ml, feeding the chaetoceros stored at low temperature in the breeding period; placing the grown infantile choline into a seedling-collecting pond internally provided with a corrugated plate where the benthic diatoms grow, and breeding the choline to reach the diameter of 3-5 mm; and peeling the infantile choline from the corrugated plate to be cultivated, and cultivating the infantile choline to grow into a young choline with the diameter of more than 1 cm, at this moment, the young choline can be used for artificial culture as the offspring seed.

Description

Sea hedgehog offspring seed cultivation method
Technical field:
The present invention relates to the breeding method of a kind of sea urchin seed, especially a kind ofly realize extra large hedgehog artificial breeding, for the raiser provides the extra large hedgehog offspring seed cultivation method of large-scale farming with seed.
Background technology:
Sea urchin (Sea Urchin) is a kind of invertebrate, be under the jurisdiction of Echinodermata, Eleutherozoa, Echinoidea, the existing kind in the whole world has 850 kinds approximately, China finds so far about 100 kinds, but the kind of economically valuable is less, and the economic species that has been developed preferably is no more than 30 kinds so far.The economic sea urchin kind of China mainly contains horsedung sea urchin, Strongylocentrotus intermedius, Strongylocentrotus nudus, sea thorn numerous (Huanghai Sea courage) in the north, south mainly contains Anthocidaris crassispina, white sour jujube three row sea urchins.The nutritive value of sea urchin mainly concentrates on the sea urchin Huang (sexual gland), contains 41g protein, 32.7g fat in every 100g urchin Huang, also contains vitamin A, D, nutrient components such as each seed amino acid and phosphorus, iron, calcium.Sea urchin also has medical value, and the sea hedgehog extractive ripple is that profit rather has anticancer growth effect, in its gonad contain eicosenoic acid and account for more than 30% of total fatty acids, eicosenoic acid is the active drug of prevention cardiovascular disease.Because the nutritive value and the medical value of sea urchin, the sea urchin demand constantly increases.
The sea thorn is numerous, has another name called Huanghai Sea courage, and formal name used at school is Glyptocidaris crenularis, is commonly called as yellow thorn bowl.In China, its geographical distribution is big Qin Dao and Dalian south sea area in Shandong mainly.Since its gonad maturity phase be annual December to next year by the end of April, and market does not have other kind sea urchin edibles during this, therefore, adopts without restraint to catch and causes natural resources exhausted day by day, people explore artificial raise seedling method always.Yet Yin Haici numerous mating season is spring and young pelagic period to grow (being generally 25~30 days), and the bait-Chaetoceros that is fit to its growth is cultivated than difficulty is extensive during this period, long-time, and this has just limited carrying out smoothly of extra large hedgehog artificial breeding.
Summary of the invention:
The present invention is in order to solve the above-mentioned technical problem of existing in prior technology, a kind of extra large hedgehog artificial breeding of realizing to be provided, for the raiser provides the extra large hedgehog offspring seed cultivation method of large-scale farming with seed.
Technical solution of the present invention is: a kind of extra large hedgehog offspring seed cultivation method is characterized in that carrying out as follows:
A. Chaetoceros is cultivated: cultivate Chaetoceros and centrifugal concentrated back cryopreservation at high temperature season;
B. benthic diatom is cultivated: collecting seedling extra large hedgehog began to cultivate benthic diatom in preceding 10~20 days, method be with corrugated plating with 0.5~1.0%NaOH aqueous solution soaking, 1~2d, rinse well with sand filter seawater, put in the pond with frame set again and in the pond, inject seawater; Gather the large-scale algae kind of intertidal zone growth, after 200~400 mesh sieve tulles filter, evenly be sprinkled in the pond and cultivate; Condition of culture is perennial draingage 3~6h every day, and streamflow is 1~2 full dose, and day is executed 0.02~0.04g/l urea, and the ratio of nutritive salt is N: P: Fe: Si=10 in the flowing water: 1: 0.1: 1; Illumination 2000~3000lx, 10~16 ℃ of water temperatures;
C. select close courage: select shell footpath 8cm at least, healthy not damaged, in 2~3 ages, the individuality that gonad development is good is done close courage;
D. adopt ovum and hatching: induce close courage to lay eggs with KCl solution stimulus method, collect smart ovum and after collection, carry out artificial insemination in 0.5~2h, after the microscopy fertilization membrane lifts, divide the pond hatching with fertilized egg by the density of 3~5/ml, trace inflation and regularly stirring between the incubation period, the hatching water temperature is 15~18 ℃, and hatching development of fertilized ova to blastaea rupture of membranes floats;
E. floating larvae is cultivated: choose the healthy larva in upper strata, cultivate by the density of 0.4~0.6/ml, cultivate 15~22d; The feed Chaetoceros of a step cryopreservation of nurturing period, scale of feeding is 1~1.5 ten thousand cell/mld, feeds every day 2~3 times; Cultivating water temperature is 13~17 ℃, begins to change water after 2~3 days, changes water 1~2 time, and changes water 1/2~2/3 at every turn in one day; Trace inflation continuously, all suctions end 1~2 time;
F. collect seedling and young courage is cultivated: the young courage that will grow up to drops into the described length of built-in b step to be had in the pond of collecting seedling of benthic diatom corrugated plating and cultivates, cultivating water temperature is 12~16 ℃, changes water 1~2 time, and changes water 1/3~2/3 at every turn in one day, trace inflation is continuously cultivated courage and is directly reached 3~5mm;
G. peeling off and intermediate rearing of young courage: begin to carry out young courage and peel off, young courage after peeling off changes the pond and cultivates, bait throwing in per 2~3 days is 1 time between culture period, throw something and feed hole sea lettuce, sea-tangle and undaria pinnitafida, cultivating water temperature is 12~16 ℃, changes water 1~2 time, and changes water 1/2~2/3 at every turn in one day, continuously trace inflation is cultured to shell and directly is the young courage of 1cm at least.
The KCl solution stimulus method of described d step is the container that the anti-actinal surface of ripe close courage that will clean places suitable bore downwards, allows the concentration be the KCl sea water solution submergence gonopore of 0.5~1.0mol/l or the KCl solution 4~6ml that injects 0.5~1.0mol/l at the membrana perisomialis of each close courage.
The present invention can realize extra large hedgehog artificial breeding, uses seed for the raiser provides large-scale farming.
Embodiment:
A. Chaetoceros is cultivated: shift to an earlier date also centrifugal concentrated back low temperature (20 ℃-4 ℃) storage of large-scale culture Chaetoceros at high temperature season, solved and carried out not enough this technical barrier of feed in the numerous production of growing seedlings on a large scale of sea thorn;
B. benthic diatom is cultivated: collecting seedling extra large hedgehog began to cultivate benthic diatom in preceding 10~20 days, method is with 0.5~1.0%NaOH aqueous solution soaking, 1~2d with corrugated plating, rinse well with sand filter seawater, neatly put into the pond and inject seawater with the framework assembling again in the pond; Gather the large-scale algae kinds such as mouse tail algae of intertidal zone growth, after 200~400 mesh sieve tulles filter, evenly be sprinkled into and cultivate in the pond; Condition of culture is perennial draingage 3~6h every day, and streamflow is 1~2 full dose, and day is executed 0.02~0.04g/l urea so that N to be provided, and the ratio of other nutritive salt and N is N: P: Fe: Si=10 in the flowing water: 1: 0.1: 1; Illumination 2000~3000lx, 10~16 ℃ of water temperatures, the nurturing period can be killed the sufficient class of scratching of numerous life on the corrugated plating with the chlorophos of 2~6mg/l;
C. select close courage: select shell footpath 8cm at least, healthy not damaged, in 2~3 ages, the individuality that gonad development is good is done close courage;
D. adopt ovum and hatching: induce close courage to lay eggs with KCl solution stimulus method, the anti-actinal surface of be about to cleaning of ripe close courage places the container of suitable bore downwards, allows the concentration be the KCl sea water solution submergence gonopore of 0.5~1.0mol/l or the KCl solution 4~6ml that injects 0.5~1.0mol/l at the membrana perisomialis of each close courage.Parent's courage is stimulating 3~6min later can discharge smart ovum, collect smart ovum and after collection, carry out artificial insemination (fertilization rate is greater than 80%) in 0.5~2h, after the most of fertilization membranes of microscopy lift, divide the pond hatching with fertilized egg by the density of 3~5/ml, trace inflation and regularly stirring between the incubation period, to improve incubation rate, the hatching water temperature is 15~18 ℃, and hatching development of fertilized ova to blastaea rupture of membranes floats;
E. floating larvae is cultivated: choose the healthy larva in upper strata, cultivate by the density of 0.4~0.6/ml, cultivate 15~22d and become young courage; The feed Chaetoceros of a step cryopreservation of nurturing period, scale of feeding is 1~1.5 ten thousand cell/mld, feeds every day 2~3 times; Cultivating water temperature is 13~17 ℃, begins to change water after 2~3 days, changes water 1~2 time, and changes water 1/2~2/3 at every turn in one day; Trace inflation continuously, all suctions end 1~2 time, are to remove foreign material such as residual bait, ight soil;
F. collect seedling and young courage is cultivated: the young courage that will grow up to drops into the described length of built-in b step to be had in the pond of collecting seedling of benthic diatom corrugated plating and cultivates, young courage then is attached to length to be had on the corrugated plating of benthic diatom, cultivating water temperature is 12~16 ℃, changed water in one day 1~2 time, change water 1/3~2/3 at every turn, trace inflation is continuously cultivated courage and is directly reached 3~5mm;
G. peeling off and intermediate rearing of young courage: young courage is stripped down from corrugated plating, young courage after peeling off changes the pond and cultivates, bait throwing in per 2~3 days is 1 time between culture period, throw something and feed hole sea lettuce, sea-tangle and undaria pinnitafida, and cultivating water temperature is 12~16 ℃, changed water in one day 1~2 time, change water 1/2~2/3, trace inflation continuously was through 3~4 months cultivation at every turn, young courage can grow to the above young courage of 1cm more than 90%, and just can be used as seed and be used to propagate artificially this moment.

Claims (2)

1. extra large hedgehog offspring seed cultivation method is characterized in that carrying out as follows:
A. Chaetoceros is cultivated: cultivate Chaetoceros and centrifugal concentrated back cryopreservation at high temperature season;
B. benthic diatom is cultivated: collecting seedling extra large hedgehog began to cultivate benthic diatom in preceding 10~20 days, method be with corrugated plating with 0.5~1.0%NaOH aqueous solution soaking, 1~2d, rinse well with sand filter seawater, put in the pond with frame set again and in the pond, inject seawater; Gather the large-scale algae kind of intertidal zone growth, after 200~400 mesh sieve tulles filter, evenly be sprinkled in the pond and cultivate; Condition of culture is perennial draingage 3~6h every day, and streamflow is 1~2 full dose, and day is executed 0.02~0.04g/l urea, and the ratio of nutritive salt is N: P: Fe: Si=10 in the flowing water: 1: 0.1: 1; Illumination 2000~3000lx, 10~16 ℃ of water temperatures;
C. select close courage: select shell footpath 8cm at least, healthy not damaged, in 2~3 ages, the individuality that gonad development is good is done close courage;
D. adopt ovum and hatching: induce close courage to lay eggs with KCl solution stimulus method, collect smart ovum and after collection, carry out artificial insemination in 0.5~2h, after the microscopy fertilization membrane lifts, divide the pond hatching with fertilized egg by the density of 3~5/ml, trace inflation and regularly stirring between the incubation period, the hatching water temperature is 15~18 ℃, and hatching development of fertilized ova to blastaea rupture of membranes floats;
E. floating larvae is cultivated: choose the healthy larva in upper strata, cultivate by the density of 0.4~0.6/ml, cultivate 15~22d; The feed Chaetoceros of a step cryopreservation of nurturing period, scale of feeding is 1~1.5 ten thousand cell/mld, feeds every day 2~3 times; Cultivating water temperature is 13~17 ℃, begins to change water after 2~3 days, changes water 1~2 time, and changes water 1/2~2/3 at every turn in one day; Trace inflation continuously, all suctions end 1~2 time;
F. collect seedling and young courage is cultivated: the young courage that will grow up to drops into the described length of built-in b step to be had in the pond of collecting seedling of benthic diatom corrugated plating and cultivates, cultivating water temperature is 12~16 ℃, changes water 1~2 time, and changes water 1/3~2/3 at every turn in one day, trace inflation is continuously cultivated courage and is directly reached 3~5mm;
G. peeling off and intermediate rearing of young courage: begin to carry out young courage and peel off, young courage after peeling off changes the pond and cultivates, bait throwing in per 2~3 days is 1 time between culture period, throw something and feed hole sea lettuce, sea-tangle and undaria pinnitafida, cultivating water temperature is 12~16 ℃, changes water 1~2 time, and changes water 1/2~2/3 at every turn in one day, continuously trace inflation is cultured to shell and directly is the young courage of 1cm at least.
2. extra large hedgehog offspring seed cultivation method according to claim 1, it is characterized in that: the KCl solution stimulus method of described d step is the container that the anti-actinal surface of ripe close courage that will clean places suitable bore downwards, allows the concentration be the KCl sea water solution submergence gonopore of 0.5~1.0mol/l or the KCl solution 4~6ml that injects 0.5~1.0mol/l at the membrana perisomialis of each close courage.
CN2010102355838A 2010-07-26 2010-07-26 Breeding method of glyptocidaris crenularis offspring seed Pending CN101940171A (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102972321A (en) * 2012-12-20 2013-03-20 广西蓝浩海洋生物科技有限公司 Purple sea urchin factory box type breeding method
CN106359207A (en) * 2016-08-31 2017-02-01 山东省海洋资源与环境研究院 Non-mechanical peeling method of young sea urchins
CN107211932A (en) * 2017-05-30 2017-09-29 赵玉明 A kind of abalone offspring seed cultivation method
CN107736283A (en) * 2017-09-22 2018-02-27 大连海洋大学 The energy-saving and emission-reduction method for culturing seedlings of blue full dress sea urchin
CN111296336A (en) * 2020-04-09 2020-06-19 海南蓝泰邦生物技术有限公司 Method for cultivating sea urchin fries of white spine three rows
CN112471014A (en) * 2020-11-25 2021-03-12 大连海洋大学 Method for breeding sea urchin seedlings through medium ball of high-density attached plates

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JPS63112940A (en) * 1986-10-31 1988-05-18 Mitsui Shipbuilding Eng Method for breeding awabi and sea urchin
JPH07184560A (en) * 1993-10-01 1995-07-25 Kaiyo Seibutsu Kenkyusho:Kk Method for raising larva of sea urchin
CN201042163Y (en) * 2007-05-22 2008-04-02 大连水产学院 Ecological cultivation case for sea urchin capable of being cultivated with sea cucumber by mix
WO2008074084A1 (en) * 2006-12-20 2008-06-26 Macquarie University Production of sea urchin roe

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JPS63112940A (en) * 1986-10-31 1988-05-18 Mitsui Shipbuilding Eng Method for breeding awabi and sea urchin
JPH07184560A (en) * 1993-10-01 1995-07-25 Kaiyo Seibutsu Kenkyusho:Kk Method for raising larva of sea urchin
WO2008074084A1 (en) * 2006-12-20 2008-06-26 Macquarie University Production of sea urchin roe
CN201042163Y (en) * 2007-05-22 2008-04-02 大连水产学院 Ecological cultivation case for sea urchin capable of being cultivated with sea cucumber by mix

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102972321A (en) * 2012-12-20 2013-03-20 广西蓝浩海洋生物科技有限公司 Purple sea urchin factory box type breeding method
CN106359207A (en) * 2016-08-31 2017-02-01 山东省海洋资源与环境研究院 Non-mechanical peeling method of young sea urchins
CN107211932A (en) * 2017-05-30 2017-09-29 赵玉明 A kind of abalone offspring seed cultivation method
CN107736283A (en) * 2017-09-22 2018-02-27 大连海洋大学 The energy-saving and emission-reduction method for culturing seedlings of blue full dress sea urchin
CN107736283B (en) * 2017-09-22 2021-05-11 大连海洋大学 Energy-saving emission-reduction seedling raising method for blue robe sea urchins
CN111296336A (en) * 2020-04-09 2020-06-19 海南蓝泰邦生物技术有限公司 Method for cultivating sea urchin fries of white spine three rows
CN112471014A (en) * 2020-11-25 2021-03-12 大连海洋大学 Method for breeding sea urchin seedlings through medium ball of high-density attached plates

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Application publication date: 20110112