CN105393909A - Sargassumvachellianum industrialized seedling production method - Google Patents
Sargassumvachellianum industrialized seedling production method Download PDFInfo
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- CN105393909A CN105393909A CN201510829691.0A CN201510829691A CN105393909A CN 105393909 A CN105393909 A CN 105393909A CN 201510829691 A CN201510829691 A CN 201510829691A CN 105393909 A CN105393909 A CN 105393909A
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- algae
- seedling
- spore
- seawater
- water
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G33/00—Cultivation of seaweed or algae
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
Abstract
The invention belongs to the biotechnology field, and especially relates to a Sargassumvachellianum industrialized seedling production method comprising the following steps: collecting algae seedling from the sea; selecting algae seedlings with obvious male/female receptacle differences from the algae seedlings, processing the selected algae seedling and removing impurities, and placing the processed algae seedlings into storage pond sea water with no other algae; culturing the algae seedlings to mature algae under the conditions of lighting intensity 80-150umol.m-2.s-1 and water temperature of 18-26 DEG C; placing mature algae into a spore collection box according to female/male ratio of 3:1, and collecting spores; configuring spore water according to spore-water ratio of 3-8g:2L, spraying the 2L spore water into the 13sq.m. seedling pond sea water with no other algae, inflating the seedling pond 48hours later, and culturing the spores into seedlings. The method is simple in operation, needs no nutrient solution, can largely cultivate Sargassumvachellianum, thus providing base for large scale Sargassumvachellianum algae field construction and sea algae ecological restoration and environment protection.
Description
Technical field
The invention belongs to biological technical field, particularly a kind of Wa Shi sargassum factorial seedling-culturing method.
Background technology
Large-scale Benthic algae has important ecology and Economic Function in marine ecosystems.Wa Shi sargassum is as the indispensable role of a kind of large-scale Benthic algae performer in marine ecosystems.Wa Shi sargassum (Sargassumvachellianum) belongs to Phaeophyta (Phaeophyta), Fucales (Fucales), Sargassaceae (Sargassaceae), Sargassum (SargassumC.Agardh).Wa Shi sargassum frond is yellowish-brown, and profile is tree-shaped, tool air bag, is perennial marine alga.Along with China's expanding economy in this year, China changes from a large agricultural country to industrial power, and a large amount of industrial wastewaters etc. are not treated to be just discharged in seawater, causes seawater eutrophication, adds the reasons such as artificial epeirogenetic in addition and cause marine ecosystems to be degenerated.Call the investigation such as sun together to show according to bavin, the Wa Shi sargassum ecosystem subordination gently degraded of Zhoushan areas, III class deteriorated Grade.In order to avoid degenerating further in Wa Shi sargassum algae field, seek a large amount of nursery of a kind of batch production method most important.And present Wa Shi sargassum cultivate be mostly laboratory experiment condition under cultivate for experimental study on a small quantity, also have no idea to reach the effect of batch production large scale cultivating.
For the weakness of prior art; the invention provides a kind of Wa Shi sargassum factorial seedling-culturing method, the present invention is simple to operate, need not prepare culture fluid; can large scale cultivating Wa Shi sargassum, extensive Wa Shi sargassum algae field is set up and Algae Ecology reparation and habitat are protected and laid a good foundation from now on.
Summary of the invention
Object of the present invention; be the shortcoming and defect overcoming said method; to Wa Shi sargassum factorial seedling-culturing method; the present invention is simple to operate; culture fluid need not be prepared; can large scale cultivating Wa Shi sargassum, extensive Wa Shi sargassum algae field is set up and Algae Ecology reparation and habitat are protected and laid a good foundation from now on.
Object of the present invention can be achieved through the following technical solutions:
A kind of Wa Shi sargassum factorial seedling-culturing method, its step comprises:
(1) algae seedling is in the sea gathered.
(2), after filtering out the algae seedling process to free from admixture that gamogenesis holder in algae seedling obviously distinguishes, the seawater without assorted algae in storage pond, holding pond is put into.
(3) be 80-150 μm of olm in intensity of illumination
-2s
-1, under the condition of water temperature 18-26 DEG C, cultivate algae seedling to ripe frond.
(4) be that 3:1 puts into spore collecting box by ripe frond according to female-male proportion, collect spore.Arrenotoky holder there will be the phenomenon that decays after discharging most of sperm, and then affects the discharge of other male and female algae strain spores, causes algae strain to decay in advance.Through investigation and comparison, the ratio of male and female 3:1 is conducive to the discharge of spore most, can take out when finding that there is male algae strain and occurring decaying, and fills into healthy male algae strain.
(5) spore and water are configured to spore fluid by 3-8g:2L, and 2L spore fluid are splashed to the 13m without assorted algae of nursery pond
2in seawater, after 48 hours, nursery pond is inflated.Such fertilized egg can guaranteed in water can well be attached on adherance, inflation in advance fertilized egg can be caused not adhere to or attachment state poor.
(6) air-charging incubation 7 days, within every 1-2 days, change the seawater of 1/4-1/3, cultivation temperature is 18-29 DEG C, and intensity of illumination is 40-120 μm of olm
-2s
-1.Initial stage due to frond seedling small and weak, change dilutional hyponatremia and seedling bulk-growth can be caused to be affected, and significantly change water due to current, hydraulic pressure etc. seedling body can be caused to come off from adherance.
(7) continue air-charging incubation 7 days, within every 1-2 days, change the seawater less than 1/2-2/3, cultivation temperature is 18-26 DEG C, and intensity of illumination is 30-100 μm of olm
-2s
-1, cultivate to seedling to spore.
Preferably, the process of algae seedling is that Seawater Treatment falls the impurity, spiral shell etc. on surface by filtered Seawater Treatment in step (2).
Preferably, the seawater without assorted algae in step (2) is that the airtight lighttight method process of seawater forms.The method of this process seawater, simply, easy to operate, also can realize easily in factory.
Preferably, the intensity of illumination in step (3) is 120 μm of olm
-2s
-1, water temperature 18-22 DEG C.
Preferably, the time of collecting spore in step (4) is 2 pm.Wa Shi sargassum usually at noon can be a large amount of diffuse spore, now collecting effect is optimum.
Preferably, the adherance such as seedbed, algal reef, Miao Sheng of the seawater that submerges is had in the nursery pond in step (5).After having seedbed, spore more easily adheres to, dispersion evenly, be beneficial to cultivation, after being cultured to finished product, directly seabed can be placed in seedbed etc., more easily reparation algae field.
Preferably, the light intensity of the nursery pond in step (5) is regulated by incandescent lamp, skylight, shadow shield etc., and temperature is by vent window and change water etc. and regulate, simple to operate, can batch production.
Preferably, the spore in step (5) and water are configured to spore fluid by 5g:2L.
Preferably, the intensity of illumination in step (6) is 100 μm of olm
-2s
-1, water temperature 18-22 DEG C.
Preferably, the intensity of illumination in step (7) is 60 μm of olm
-2s
-1, water temperature 18-22 DEG C.
Described preparation method is applied to biological technical field.
The invention has the beneficial effects as follows:
To Wa Shi sargassum factorial seedling-culturing method, the present invention is simple to operate, need not prepare culture fluid, can large scale cultivating Wa Shi sargassum, and extensive Wa Shi sargassum algae field is set up and Algae Ecology reparation and habitat are protected and laid a good foundation from now on.
Embodiment
Below in conjunction with embodiment, the invention will be further described:
1, collect seedling
Zhoushan areas Wa Shi sargassum breeding month is the 6-7 month, and in April, we just can see the formation having receptacle.From the May, dive under water weekly once, gather Wa Shi sargassum algae seedling, observe the mature condition of receptacle, and laboratory microexamination is taken back in collection on a small quantity.Receptacle initial stage gamogenesis holder no significant difference, along with growth, receptacle difference is obvious, can obviously distinguish on the basis of gamogenesis holder, collects seedling in a large number.Gather the algae strain of returning first to support temporarily in storage pond, holding pond, with the seawater filtered in advance, whole seedling body is rinsed before putting into nursery pond, removing surface impurity, spiral shell etc.This is only first employing, along with the carrying out of our nursery activity, we can select high-quality algae seedling for conservation in cultivating process, avoid the cost of repeatedly collecting seedling and the destruction to natural algae field, and can by thinking that the whole year is carried out nursery in regulating and controlling temperature and illumination.
2, spore is collected
The process of 2.1 seawater
Breeding seawater is directly from sea area.First seawater is added cistern, cistern size is the cylindrical structural of diameter 5m, and space, cistern place is complete airtight light tight, and the water adding cistern can not be directly used in nursery.Because the airtight lighttight characteristic of cistern, can be used for spore and the seedling body of killing other algae, prevent seedling raising process from affecting the nursery of Wa Shi sargassum due to the intervention of assorted algae.
Seawater may be used for nursery in about one week in cistern process, also needs by filter before passing into nursery pond, and the inside of filter is paved with various filtering material, is followed successively by shell, nitrated ring, sandstone, sponge formation from top to bottom.The long 3m of filter, wide 3m, degree of depth 2m.Another nursery pond size is long 3m, wide 1.5m, the degree of depth 0,85m.
The preparation of 2.2 artificial adherances
Seedbed is made up of seedling rope and framework two parts, and each nursery pond lays a seedbed, and seedbed size is long 2.5m, wide 1.3m.Seedling rope is wrapped on framework, puts it into after seedbed prepares in nursery pond, corner, seedbed is connected with nursery pond by fixture, prevent seedbed in seedling raising process from raising and even emerge.
Algal reef is made up of (55m*55m*5m) cement and sandstone, and algal reef is also laid in the bottom after having prepared.
The collection of 2.3 spores
Gather the Wa Shi sargassum algae strain come up and cross the maturation accelerating receptacle under light filling mends temperature (intensity of illumination reaches 120 μm of olm-2s-1, water temperature 23 DEG C) condition in manual intervention.Every day, 2:00 collection nursery pond water sample was observed in the afternoon, started when observing fertilized egg and occurring the collection preparing spore.
By the frond of maturation according to male and female 3:1 ratio put into spore collecting box, every afternoon, 2:00 carried out the collection of spore by yarn thin,tough silk filter.The spore collected is configured to the spore fluid full pool spilling head of certain density.
3, cultivate
Before spore is splashed, whole nursery pond stops inflation, can recover inflation, facilitate fertilized egg can well be attached to algal reef and Miao Shengshang after the 48h that splashes.After splashing in one week, change water amplitude at every turn and should not exceed 1/3 of whole water body, the interchangeable water 2/3 of second week, after be can be observed Wa Shi sargassum seedling by naked eyes, water is changed in full pond, avoids frond due to anoxic, lacks the poor growths such as nutrition.Change the water time is 3:00 in afternoon at every turn
Cultivate first week, the suitableeest illumination of Wa Shi sargassum is 100 μm of olm-2s-1, and second week starts, and the most suitable growth light intensity is 60 μm of olm-2s-1, and temperature controls below 26 DEG C.
4, the management of nursery pond
Each seedling rearing room tool 8 skylights, light transmission is good, and indoor have the incandescent lamp 8 of 30W, one, air-conditioning.Regulate by shadow shield when light is crossed strong, when illumination is not enough, incandescent lamp light filling can be opened.Temperature aspect regulates by window, change water and air-conditioning.While nursery, every day, right place monitored temperature in indoor illumination intensity and nursery pond, will adjust immediately when there is illumination deficiency and temperature drift.
Test result:
This Wa Shi sargassum factorial seedling-culturing method, passes through into the processes such as algae gathers, egg cell diffuses, is fertilized, set, sprouting, seedling culture, cultivates about 3,600,000 seedlings altogether.On average every gram of adult female Wa Shi sargassum can discharge 5415 egg cells, and after fertilization 48h, fertilized egg base portion forms 8-11 bar rhizoid; Algal reef plate fertilized egg adhesive rate is up to 92.59%, and seedling rope and net bed adhesive rate are 7/10cm; Target marine site is moved to after seedling average length is 5mm, after throwing in sea area by diving, seedling grows fine, the longest 6cm that reaches of seedling after 45 days, and because algal reef, seedling rope and seedbed constitute complex environment structure, perch and protect place for the biologies such as fish provide, enrich species diversity.This sets up for extensive Wa Shi sargassum algae field from now on and Algae Ecology reparation and habitat are protected and laid a good foundation.
The above is preferred embodiment of the present invention, but the present invention should not be confined to the content disclosed in this embodiment.The equivalence completed under not departing from spirit disclosed in this invention so every or amendment, all fall into the scope of protection of the invention.
Claims (10)
1. a Wa Shi sargassum factorial seedling-culturing method, its step comprises:
(1) algae seedling is in the sea gathered;
(2), after filtering out the algae seedling process to free from admixture that gamogenesis holder in algae seedling obviously distinguishes, the seawater without assorted algae in storage pond, holding pond is put into;
(3) be 80-150 μm of olm in intensity of illumination
-2s
-1, under the condition of water temperature 18-26 DEG C, cultivate algae seedling to ripe frond;
(4) be that 3:1 puts into spore collecting box by ripe frond according to female-male proportion, collect spore;
(5) spore and water are configured to spore fluid by 3-8g:2L, and 2L spore fluid are splashed to the 13m without assorted algae of nursery pond
2in seawater, after 48 hours, nursery pond is inflated;
(6) air-charging incubation 7 days, within every 1-2 days, change the seawater of 1/4-1/3, cultivation temperature is 18-29 DEG C, and intensity of illumination is 40-120 μm of olm
-2s
-1;
(7) continue air-charging incubation 7 days, within every 1-2 days, change the seawater of 1/2-2/3, cultivation temperature is 18-26 DEG C, and intensity of illumination is 30-100 μm of olm
-2s
-1, cultivate to seedling to spore.
2. Wa Shi sargassum factorial seedling-culturing method according to claim 1, is characterized in that: the process of algae seedling is by filtered Seawater Treatment in step (2).
3. Wa Shi sargassum factorial seedling-culturing method according to claim 1, is characterized in that: the seawater without assorted algae in step (2) is that the airtight lighttight method process of seawater forms.
4. Wa Shi sargassum factorial seedling-culturing method according to claim 1, is characterized in that: the intensity of illumination in step (3) is 120 μm of olm
-2s
-1, water temperature 18-22 DEG C.
5. Wa Shi sargassum factorial seedling-culturing method according to claim 1, is characterized in that: the time of collecting spore in step (4) is 2 pm.
6. Wa Shi sargassum factorial seedling-culturing method according to claim 1, is characterized in that: have the seedbed of the seawater that submerges, algal reef, Miao Sheng in the nursery pond in step (5).
7. Wa Shi sargassum factorial seedling-culturing method according to claim 1, is characterized in that: the light intensity of the nursery pond in step (5) is regulated by incandescent lamp, temperature is by ventilating and changing water to regulate.
8. Wa Shi sargassum factorial seedling-culturing method according to claim 1, is characterized in that: the spore in step (5) and water are configured to spore fluid by 5g:2L.
9. Wa Shi sargassum factorial seedling-culturing method according to claim 1, is characterized in that: the intensity of illumination in step (6) is 100 μm of olm
-2s
-1, water temperature 18-22 DEG C.
10. Wa Shi sargassum factorial seedling-culturing method according to claim 1, is characterized in that: the intensity of illumination in step (7) is 60 μm of olm
-2s
-1, water temperature 18-22 DEG C.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106069712A (en) * | 2016-07-04 | 2016-11-09 | 广东海洋大学 | Suspend based on the Alga Sgrgassi Enerves seedling scale of amphigenetic Leizhou and aestivate breeding method |
| CN110291983A (en) * | 2019-06-26 | 2019-10-01 | 山东省海洋生物研究院 | A kind of Inoculation Method of sargassum kjellmanianum Yendo algal reef |
| CN113508767A (en) * | 2021-04-29 | 2021-10-19 | 上海海洋大学 | Method for repairing seaweed field by culturing shellfish-algae complex |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103202214A (en) * | 2013-04-26 | 2013-07-17 | 上海海洋大学 | Breeding method of sargassum vachellianum artificial seeds |
| CN103385170A (en) * | 2013-07-14 | 2013-11-13 | 上海海洋大学 | A seedling cultivation method for Sargassum vachellianum greville by using tissue culture |
-
2015
- 2015-11-25 CN CN201510829691.0A patent/CN105393909A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103202214A (en) * | 2013-04-26 | 2013-07-17 | 上海海洋大学 | Breeding method of sargassum vachellianum artificial seeds |
| CN103385170A (en) * | 2013-07-14 | 2013-11-13 | 上海海洋大学 | A seedling cultivation method for Sargassum vachellianum greville by using tissue culture |
Non-Patent Citations (1)
| Title |
|---|
| ZHAOYANG CHAI ETAL: "Studies on breeding of Sargassumvachellianumon artificial reefs in Gouqi Island, China", 《AQUACULTURE》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106069712A (en) * | 2016-07-04 | 2016-11-09 | 广东海洋大学 | Suspend based on the Alga Sgrgassi Enerves seedling scale of amphigenetic Leizhou and aestivate breeding method |
| CN106069712B (en) * | 2016-07-04 | 2020-02-18 | 广东海洋大学 | Large-scale suspension summer-passing cultivation method for Sargassum leizhou seedlings based on sexual reproduction |
| CN110291983A (en) * | 2019-06-26 | 2019-10-01 | 山东省海洋生物研究院 | A kind of Inoculation Method of sargassum kjellmanianum Yendo algal reef |
| CN110291983B (en) * | 2019-06-26 | 2021-09-14 | 山东省海洋生物研究院 | Artificial inoculation method for sargassum muticum reef |
| CN113508767A (en) * | 2021-04-29 | 2021-10-19 | 上海海洋大学 | Method for repairing seaweed field by culturing shellfish-algae complex |
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Application publication date: 20160316 |