CN101755665B - Full-artificial seed cultivation method of sargassum horneri - Google Patents
Full-artificial seed cultivation method of sargassum horneri Download PDFInfo
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- CN101755665B CN101755665B CN2008102495484A CN200810249548A CN101755665B CN 101755665 B CN101755665 B CN 101755665B CN 2008102495484 A CN2008102495484 A CN 2008102495484A CN 200810249548 A CN200810249548 A CN 200810249548A CN 101755665 B CN101755665 B CN 101755665B
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- sargassum horneri
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- seed
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
Abstract
The invention relates to a cultivation method of economic seaweed, in particular to a full-artificial seed cultivation method for sargassum horneri. Concretely, the method comprises the following steps: selecting male and female sargassum horneri parent strains with good pyrenium growth to be cultivated for 10 to 20 days under the conditions that the illumination intensity is between 2500 and 10000 luxes and the temperature is between 10 and 16 DEG C for realizing the synchronous sexual development and sexual maturation of the sargassum horneri so that ova and sperms are discharged synchronously for impregnation; completing the collection and the seedling distribution of germ cells in 10 to 24 hours after the impregnation; carrying out static cultivation for 2 to 4 days after the seedling distribution; and maintaining the water flowing under the condition of the illumination intensity between 400 and 8000 luxes for completing the cultivation. The seed cultivation process of the invention is carried out in an artificial cultivation system, so the impregnation rate can be further and greatly improved, the spatfall efficiency and the full-artificial seed cultivation efficiency can be improved.
Description
Technical field
The present invention relates to the breeding method of economical alga, a kind of specifically full-artificial seed cultivation method of sargassum horneri.
Background technology
Sargassum horneri Latin title is Sargassum horneri, is that China is coastal, and especially the coastal important economical alga in subtropics is the important source material of algin, is the aquatic animal feed important component part.At present cuprum algae artificial raise seedling technique is to use the wild of intertidal zone self-sow to plant vegetables, or cultivation at the individuality of Miao Shengshang as the parent, rule of thumb judge parent's ovulation period when growing seedlings, collect fertilized egg.After having surpassed the regular hour, the coming off naturally of fertilized egg causes the progeny size that obtains seldom, perhaps misses the best period of collecting seedling at all.In addition, the asynchronous offspring of causing of fertilization is in different developmental phases, makes the later stage take off seedling easily; Dioecious biological property has determined that parent's fertilization rate in these two kinds of sources is low, and offspring embryo and development of fertilized ova are asynchronous, have caused breeding efficiency low.
Summary of the invention
The invention provides a kind of fertilization rate height, full-artificial seed cultivation method of sargassum horneri that spatfall efficient is high.
For achieving the above object, the technical solution used in the present invention is:
Breeding method plants vegetables:
1) ovum and sperm discharge synchronously: nutritive salt was cultivated 10-20 days and added to the female male parent of the Sargassum horneri chosen under the intensity of illumination of 2500-10000 lux, 10-16 ℃ temperature condition, realize the female male parent synchronism growth of Sargassum horneri and ripe, ovum and sperm are discharged synchronously, be fertilized; Nutritive salt: adopt the seawater preparation wherein to contain NaNO
3: 1-10ppm; KH
2PO
4: 0.1-1ppm.
2) seed is cultivated: in after fertilization 10-24 hour, finish the collection and the cloth seedling of fertilized egg, treated behind the cloth seedling static cultivations 2-4 days, under the illumination of 4000-8000 lux, and change a water in 24 hours, maintenance flowing water is finished cultivation.
The female male parent of the described Sargassum horneri of choosing is for choosing the female male parent of the well-developed Sargassum horneri of receptacle.Described female male parent ratio is 10-20: 1-2.During described cloth seedling according to the density cloth seedling of every square centimeter of 3-6 fertilized egg.Described step 2) seed inoculates in needs after the static cultivation on the adherance inflate or the flowing water cultivation culture environment, cultivates after 7-10 days, to culture environment flushing 1 time, waits to cultivate when seedling length is 1-5 centimetre and plunges into the commercial sea.
The advantage that the present invention had:
1. in the seed rearing process of the present invention in the artificial culture system, cultivate the female male parent of Sargassum horneri, make that ovum and diffusing of sperm have high level of synchronization in the sexual reproduction process, adopt synchronous fertilized egg and the embryo of growth to collect seedling, and then can increase substantially fertilization rate, reach more than 95%, improve spatfall efficient, fertilized egg or embryo attachment rate can reach 99%, improve the cultivation efficient of total man worker seed: can utilize 100 kilograms plant vegetables to cultivate about 100,000,000 seedling, increase substantially breeding efficiency.
2. the present invention controls fertilization process, can autotelic selection and control Sargassum horneri parent, realize the heterosis utilization of different geographical isolates, simultaneously controlled fertilized egg is in the same developmental stage and takes off the low phenomenon of seedling rate, realize in addition utilizing a large amount of offspring seedling of a spot of parent culture, broken away from uncontrollable natural dish seedling method.
Embodiment
Embodiment 1
1) ovum and sperm discharge synchronously: choose the female male parent of the well-developed Sargassum horneri of receptacle and under the intensity of illumination of 2500 luxs and 12 ℃ of temperature, cultivated 20 days,, realize that the Sargassum horneri synchronism is grown and ripe, ovum and sperm are discharged synchronously, be fertilized; Described female male parent ratio be 10: 1 (during add nutritive salt NaNO
3: 5ppm; KH
2PO
4: 0.3ppm).
2) seed is cultivated: at after fertilization in after fertilization 24 hours, finish the collection and the cloth seedling of fertilized egg, fertilized egg adopts natural subsidence and stirring method to collect, and according to the density cloth seedling of every square centimeter of 3-4 fertilized egg, treated behind the cloth seedling static cultivation 48 hours, spatfall 2 days and under the illumination of 4000 luxs is fallen on the adherance in seed inoculation, then culture environment is inflated the cultivation of adopting conventional gas stone aeration and make the flowability of cultivating water in the pond, cultivate after 10 days, culture environment is washed 1 time, wait to cultivate seedling length and plunge into the commercial sea to 1-2 centimetre.
Embodiment 2
1) ovum and sperm discharge synchronously: will choose the female male parent of the well-developed Sargassum horneri of receptacle and cultivate 10 days under the intensity of illumination of 10000 luxs and 15 ℃ of temperature, and realize that the Sargassum horneri synchronism will be grown and ripe, ovum and sperm are discharged synchronously, and be fertilized; Described female male parent ratio be 20: 1 (during add nutritive salt NaNO
3: 10ppm; KH
2PO
4: 1ppm).
2) seed is cultivated: at after fertilization in after fertilization 24 hours, finish the collection and the cloth seedling of fertilized egg, fertilized egg adopts natural subsidence and stirring method to collect, and according to the density cloth seedling of every square centimeter of 3-4 fertilized egg, treated behind the cloth seedling static cultivation 4 days, seed be inoculated on the adherance, and under the illumination of 8000 luxs spatfall 4 days, then carry out continuous productive process to cultivating the pond, minimum the reaching of streamflow changed the cultivation seawater every day one time.Cultivate after 7 days, culture environment is washed 1 time, wait to cultivate seedling length and plunge into the commercial sea to 3-4 centimetre.
Embodiment 3
1) ovum and sperm discharge synchronously: will choose the female male parent of the well-developed Sargassum horneri of receptacle and cultivate 15 days under the intensity of illumination of 5000-7500 lux and 16 ℃ of temperature, realize that the Sargassum horneri synchronism is grown and maturation, ovum and sperm are discharged synchronously, be fertilized; Described female male parent ratio be 15: 2 (during add nutritive salt NaNO
3: 1ppm; KH
2PO
4: 0.1ppm).
2) seed is cultivated: in after fertilization 24 hours, finish the collection and the cloth seedling of fertilized egg, fertilized egg adopts natural subsidence and stirring method to collect, and according to the density cloth seedling of every square centimeter of 5-6 fertilized egg, treated behind the cloth seedling static cultivation 3 days, seed be inoculated on the adherance, and under the illumination of 8000 luxs spatfall 3 days, then carry out continuous productive process to cultivating the pond, minimum the reaching of streamflow changed the cultivation seawater every day one time.Cultivate after 8 days, culture environment is washed 1 time, wait to cultivate seedling length and plunge into the commercial sea to 4-5 centimetre.
Embodiment 4
1) ovum and sperm discharge synchronously: will choose the female male parent of the well-developed Sargassum horneri of receptacle and cultivate 18 days under the intensity of illumination of 8000-10000 lux and 10 ℃ of temperature, realize that the Sargassum horneri synchronism is grown and maturation, ovum and sperm are discharged synchronously, be fertilized; Described female male parent ratio be 10-20: 1-2 (during add nutritive salt NaNO
3: 8ppm; KH
2PO
4: 0.4ppm).
2) seed is cultivated: in after fertilization 18 hours, finish the collection and the cloth seedling of fertilized egg, fertilized egg adopts natural subsidence and stirring method to collect, and according to the density cloth seedling of every square centimeter of 3-5 fertilized egg, treated behind the cloth seedling static cultivation 2 days, seed be inoculated on the adherance, and under the illumination of 6000 luxs spatfall 2 days, then carry out continuous productive process to cultivating the pond, minimum the reaching of streamflow changed the cultivation seawater every day one time.Cultivate after 9 days, culture environment is washed 1 time, wait to cultivate seedling length and plunge into the commercial sea to 3-5 centimetre.
Claims (5)
1. full-artificial seed cultivation method of sargassum horneri is characterized in that:
1) ovum and sperm discharge synchronously: with the female male parent of the Sargassum horneri chosen under the intensity of illumination of 2500-10000 lux, add nutritive salt under the temperature of 10-16 ℃ and cultivated 10-20 days, realize the female male parent synchronism growth of Sargassum horneri and ripe, ovum and sperm are discharged synchronously, be fertilized; Nutritive salt: adopt the seawater preparation wherein to contain NaNO
3: 1-10ppm; KH
2PO
4: 0.1-1ppm;
2) seed is cultivated: in after fertilization 10-24 hour, finish the collection and the cloth seedling of fertilized egg, static cultivation, described static cultivation is to cultivate 2-4 days, under the illumination of 4000-8000 lux, and changes a water in 24 hours, keep flowing water to cultivate afterwards, finish cultivation.
2. by the described full-artificial seed cultivation method of sargassum horneri of claim 1, it is characterized in that: the female male parent of the described Sargassum horneri of choosing is for choosing the female male parent of the well-developed Sargassum horneri of receptacle.
3. by the described full-artificial seed cultivation method of sargassum horneri of claim 1, it is characterized in that: described female male parent ratio is 10-20: 1-2.
4. by the described full-artificial seed cultivation method of sargassum horneri of claim 1, it is characterized in that: during described cloth seedling according to the density cloth seedling of every square centimeter of 3-6 fertilized egg.
5. by the described full-artificial seed cultivation method of sargassum horneri of claim 1, it is characterized in that: seed is inoculated into described step 2) needs after the static cultivation on the adherance culture environment is inflated or flowing water, seed is cultivated under inflation or discharge condition, cultivate after 7-10 days, to culture environment flushing 1 time, wait to cultivate when seedling length is 1-5 centimetre and plunge into the commercial sea.
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| CN2008102495484A CN101755665B (en) | 2008-12-23 | 2008-12-23 | Full-artificial seed cultivation method of sargassum horneri |
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| CN2008102495484A CN101755665B (en) | 2008-12-23 | 2008-12-23 | Full-artificial seed cultivation method of sargassum horneri |
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| Publication Number | Publication Date |
|---|---|
| CN101755665A CN101755665A (en) | 2010-06-30 |
| CN101755665B true CN101755665B (en) | 2011-08-03 |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102640698B (en) * | 2012-05-01 | 2013-04-24 | 山东东方海洋科技股份有限公司 | Artificial seedling method for sargassum pallidum |
| CN103202214B (en) * | 2013-04-26 | 2015-03-11 | 上海海洋大学 | Breeding method of sargassum vachellianum artificial seeds |
| CN103385170B (en) * | 2013-07-14 | 2015-07-01 | 上海海洋大学 | A seedling cultivation method for Sargassum vachellianum greville by using tissue culture |
| CN103858746B (en) * | 2014-03-27 | 2016-08-17 | 青岛崂好人海洋生物技术有限公司 | A kind of algae culturing breeding method |
| CN104145946A (en) * | 2014-06-30 | 2014-11-19 | 中国科学院海洋研究所 | Method for preserving sargassum horneri fertilized eggs at low temperature |
| CN106857226A (en) * | 2017-03-03 | 2017-06-20 | 山东省海洋生物研究院 | A kind of method of Sargassum horneri in northern productivity artificial breeding |
| CN107232051B (en) * | 2017-07-21 | 2020-07-31 | 山东省海洋生物研究院 | Nutrient rapid propagation-based Sargassum horneri large-scale cultivation method |
| CN109105248B (en) * | 2018-08-29 | 2021-04-02 | 湖北工业大学 | Indoor germination method of stonewort propagules |
| CN115399236A (en) * | 2022-10-11 | 2022-11-29 | 北京雷力海洋生物新产业股份有限公司 | Seedling raising method for sargassum horneri |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101176420A (en) * | 2007-11-07 | 2008-05-14 | 孙庆海 | Cuprum algae artificial raise seedling technique |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101176420A (en) * | 2007-11-07 | 2008-05-14 | 孙庆海 | Cuprum algae artificial raise seedling technique |
Non-Patent Citations (1)
| Title |
|---|
| 孙建璋等.铜藻Sargassum horneri繁殖生物学及种苗培育研究.《南方水产》.2008,第4卷(第2期),全文. * |
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Granted publication date: 20110803 Termination date: 20171223 |