CN101129131B - Non-asepsis seedling re-cutting seedling-producing method of hippeastrum - Google Patents
Non-asepsis seedling re-cutting seedling-producing method of hippeastrum Download PDFInfo
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- CN101129131B CN101129131B CN200710046481XA CN200710046481A CN101129131B CN 101129131 B CN101129131 B CN 101129131B CN 200710046481X A CN200710046481X A CN 200710046481XA CN 200710046481 A CN200710046481 A CN 200710046481A CN 101129131 B CN101129131 B CN 101129131B
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Abstract
The invention discloses a recutting method of sterile Hippeastrum seed in the garden plant and ornamental plant domain, which is characterized by the following: adopting the sterile seed through tissue culture as explant; cutting into small bulbs; culturing; breeding; obtaining offsprings continuously; adjusting pH value of the culture medium; adding plant growing inhibitor to improve the content of phosphor and potassium; accelerating the inducing rate and thick seed; improving the growing and breeding speed of Hippeastrum; adopting half MS culture medium to replace MS culture medium to reduce the cost; improving the survival rate of seed through two-step seed-transplanting pattern.
Description
Technical field
The present invention relates to a kind of Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait aseptic seedling and cut into seedling-growing method again, improve the breeding and the growth rate of Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait tissue cultivating seedling, belong to the Ornamental Plants and Horticulture field in the agricultural biotechnologies.
Background technology
Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait (Hippeastrum) another name amaryllis, descendants of a nobleman orchid, Afriocan agapanthus etc. are that the Amaryllidaceae amaryllis belongs to herbaceos perennial, originate in Peru and Brazil's one band, are the flowering bulbs of viewing and admiring of extensive use all over the world.China is during 1912～1949 years, and ground such as Nanjing, Shanghai have the potted plant of Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait and view and admire.But high-grade Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait breeding difficulty, China's Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait kind ball relies on from state's imports such as Holland always.At present, be quick major technique means of breeding of Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait in the world by tissue culture or bulb cottage propagation.
The bulb of Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait, blade and scape can both can produce seedling through inducing.For many years, scholars are studying best inducing culture always.Though different kinds needs different prescriptions, they induce mode similar, and the hormone of interpolation is also similar, just the concentration difference.Inducing culture is: MS+BA (6-benzylamino adenine) 2～4mg/L+NAA (methyl) 0～1mg/L, active carbon (AC) 2g/L, sucrose 30g/L, Ph5.8, agar 6～8g/L.Root media is not for adding the MS medium of hormone.
The method for inducing and cultivating cycle of existing Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait seedling is long, efficient is lower, and cost can not satisfy batch production, requirement of massive production also than higher.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art, provide a kind of Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait aseptic seedling to cut into seedling-growing method again, can be fast and effeciently a large amount of production high-quality Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait seedlings are fit to batch production production.
For realizing such purpose, in the technical scheme of the present invention, the aseptic seedling of utilizing the Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait tissue culture to produce is explant, by repeating to cut clove, cultivation, breeding, constantly obtains filial generation.In inducing into seedling and strong seedling culture process, regulate pH value, the sucrose concentration of medium, the technological means such as content of adding plant growth inhibitor and improving phosphorus, potassium element in the medium, improve growth of Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait tissue cultivating seedling and proliferative speed at inductivity and the strong sprout of promotion clove.Adopt the 1/2MS medium to replace the MS medium simultaneously to reduce cost.Transplant seedlings and then adopt the two step modes of transplanting seedlings to improve seedling percent.
Method concrete steps of the present invention are:
1, the acquisition of aseptic seedling: select the hippeastrum bulb of improved seeds to carry out tissue culture.Medium is: 1/2 MS+BA2mg/L+NAA 1mg/L, pH5.8, sugared 30g/L, AC 2g/L, agar 6～8g/L; Condition of culture is: 20～30 ℃ of temperature, illumination: dark phase of 16h photophase/8h.Can obtain aseptic seedling after one month.
2, the cutting of aseptic seedling: when the aseptic seedling bulb diameter that obtains reaches 3～5mm, can cut, the aseptic seedling after the cutting is continued inducing culture.Inducing culture is: 1/2MS+BA 4mg/L+NAA1mg/L+KH
2PO
440～120mg/L+TIBA (Triiodobenzoic acid) 0.5mg/L, pH 5.8～7.0, sucrose 30～60g/L, AC 2g/L, agar 6～8g/L.Condition of culture is: 20～30 ℃ of temperature, illumination: dark phase of 16h photophase/8h.The general aseptic seedlings that can access long 1cm in about 1 month.
3, seedling strong sprout: the aseptic seedlings to long 1cm is carried out strong seedling culture, and medium is 1/2MS+BA4mg/L+NAA 1mg/L+KH
2PO
460mg/L+TIBA 0.5mg/L, pH 5.8～7.0, sucrose 30～60g/L, AC 2g/L, agar 6～8g/L.Condition of culture is: 20～30 ℃ of temperature, illumination: dark phase of 16h photophase/8h.
4, the cutting again of aseptic seedling: when the aseptic seedling clove diameter after the strong seedling culture that obtains reaches 3～5mm, wherein a part of aseptic seedling continuation cutting can be carried out new inducing culture and strong seedling culture to the aseptic seedling after the cutting again; So repeat constantly to obtain filial generation.The aseptic seedling that another part no longer cuts is transplanted seedlings.
5. transplant seedlings: will migrate in the vermiculite through the aseptic seedling of strong seedling culture and no longer cutting, keep the moisture abundance, treat the open country of to transplant seedlings once more when seedling leaves is grown to 5～7cm.When transplanting seedlings, environmental temperature is generally 10～30 ℃, and avoids the sunlight direct projection more than 10 degree.
The aseptic seedling that the present invention utilizes the Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait tissue culture to produce is explant, by repeating to cut clove, cultivation, breeding, constantly obtains filial generation.This technology does not reduce fertility with the increase of clove cutting number of times, therefore, just can continue to cut to a certain size back whenever Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait aseptic seedling clove is long, and what can not be subject to seasonal restrictions constantly obtains a large amount of high-quality Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait seedlings.The Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait seedling rooting rate that produces can reach 100%, does not therefore need to carry out culture of rootage.Carry out tissue culture with mother bulb that direct utilization is carried disease germs and compare, the present invention has reduced complex processes such as sterilization, has shortened the cycle of inducing into seedling, has simplified the flow process of producing.In inducing into seedling and strong seedling culture process, by regulating acid-base value, the sucrose concentration of medium, add means such as potassium dihydrogen phosphate and Triiodobenzoic acid, promote inductivity and the strong sprout of clove.Adopt the 1/2MS medium to replace the MS medium effectively to reduce production costs.The process in strong sprout of seedling also is a critical step, and it can replenish the nutritive element that growth of seedling needs rapidly, makes it fast rapid-result seedling and promotes clove to grow up.Strong clove not only helps cutting next time, and seedling survives after also helping transplanting seedlings.Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait vitality is more intense, can directly aseptic seedling be migrated in the vermiculite, treats the open country of can transplanting seedlings once more after seedling grows up.Transplant seedlings with the mode that two steps transplanted seedlings, can improve the survival rate of seedling.
Can be the fast and effeciently a large amount of production high-quality Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait seedlings of the present invention are methods that a kind of suitable batch production of novel practical is produced.
Embodiment
Below by specific embodiment technical scheme of the present invention is further described.Following examples do not constitute limitation of the invention.
Embodiment 1
1, the acquisition of aseptic seedling: select good Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait kind apple flower (Apple Blossom) bulb to carry out tissue culture, divest the old root of outer field old scale and bottom earlier, with cutting top 2/3 scale behind the clear water wash clean, the scale and the basal disc 8～16 of base portion are cut.Respectively with sterilization inoculation after suds and the flushing with clean water.
Medium is 1/2 MS+BA 2mg/L+NAA 1mg/L, pH5.8, sugared 30g/L, AC2g/L, agar 6g/L.Condition of culture is: 25 ℃ of temperature, illumination: dark phase of 16h photophase/8h.Produce aseptic seedling after one month.
2, the cutting of aseptic seedling: when aseptic seedling bulb diameter reaches 3～5mm, be that material cuts with the aseptic seedling clove that obtains, and the aseptic seedling after the cutting is continued inducing culture, inducing culture is: 1/2MS+BA 4mg/L+NAA 1mg/L+KH
2PO
450mg/L+ Triiodobenzoic acid 0.5mg/L regulates pH value to 6.6, adds sucrose 45g/L, AC 2g/L, agar 6g/L.Condition of culture is: 20～30 ℃ of temperature, illumination: dark phase of 16h photophase/8h; Obtain the aseptic seedlings of long 1cm.
3, seedling strong sprout: the aseptic seedlings to long 1cm is carried out strong seedling culture, and medium is 1/2MS+BA4mg/L+NAA 1mg/L+KH
2PO
460mg/L+TIBA 0.5mg/L, pH6.2, sucrose 60g/L, AC 2g/L, agar 8g/L; Condition of culture is: 20～30 ℃ of temperature, illumination: dark phase of 16h photophase/8h.
4, the cutting again of aseptic seedling: when the aseptic seedling clove diameter after the strong seedling culture that obtains reaches 3～5mm, wherein a part of aseptic seedling is continued cutting, again the aseptic seedling after the cutting is carried out new inducing culture and strong seedling culture.So repeat cutting, cultivation, strong sprout, can constantly obtain the filial generation of Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait kind apple flower.In the time of need transplanting seedlings, the aseptic seedling that can select arbitrarily no longer to cut after strong seedling culture is transplanted seedlings.
5, transplant seedlings: the aseptic seedling of a part through strong seedling culture and no longer cutting migrated in the vermiculite, and keep the skin wet every day, keeps the moisture abundance, when treating that seedling leaves is grown to 5～7cm, once more the Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait seedling is transplanted to open country from vermiculite.In summer, be chosen in the cultivation of protection ground when transplanting seedlings for the first time, suitably shade, avoid the sunlight direct projection, make environmental temperature below 30 ℃.One week back removal shading thing, these Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait seedlings just can normal growth.
Embodiment 2
1, the acquisition of aseptic seedling: select good Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait kind Wella (Vera) bulb to carry out tissue culture,
Medium is 1/2 MS+BA 2mg/L+NAA 1mg/L, pH5.8, sugared 30g/L, AC2g/L, agar 6g/L.Condition of culture is: 25 ℃ of temperature, illumination: dark phase of 16h photophase/8h.Produce aseptic seedling after one month.
2, the cutting of aseptic seedling: cut when the aseptic seedling bulb diameter that obtains reaches 3～5mm, the aseptic seedling after the cutting is continued inducing culture, inducing culture is: 1/2MS+BA 4mg/L+NAA1mg/L+KH
2PO
460mg/L+ Triiodobenzoic acid 0.5mg/L, pH6.8, sucrose 40g/L, AC 2g/L, agar 8g/L; Condition of culture is: 20～30 ℃ of temperature, illumination: dark phase of 16h photophase/8h; Obtain the aseptic seedlings of long 1cm;
3, seedling strong sprout: the aseptic seedlings to long 1cm is carried out strong seedling culture, and medium is 1/2MS+BA4mg/L+NAA 1mg/L+KH
2PO
480mg/L+TIBA 0.5mg/L, pH6.0, sucrose 50g/L, AC 2g/L, agar 8g/L.Condition of culture is: 20～30 ℃ of temperature, illumination: dark phase of 16h photophase/8h;
4, the cutting again of aseptic seedling: when the aseptic seedling clove diameter after the strong seedling culture that obtains reaches 3～5mm, wherein a part of aseptic seedling is continued cutting, again the aseptic seedling after the cutting is carried out new inducing culture and strong seedling culture; So repeat cutting, cultivation, strong sprout, can constantly obtain the filial generation of Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait kind Wella.Simultaneously, the aseptic seedling of selecting a part no longer to cut after strong seedling culture is transplanted seedlings.
5, transplant seedlings: the aseptic seedling of a part through strong seedling culture and no longer cutting migrated in the vermiculite, and keep the skin wet every day, keeps the moisture abundance.Treat the open country of transplanting seedlings once more when seedling leaves is grown to 5～7cm.In the winter time, be chosen in the cultivation of protection ground when transplanting seedlings for the first time, environmental temperature remains on more than 10 ℃.Once week these Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait seedlings energy normal growths of back.
Claims (1)
1. a Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait aseptic seedling cuts into seedling-growing method again, it is characterized in that comprising the steps:
1) acquisition of aseptic seedling: select hippeastrum bulb to carry out tissue culture, medium is: 1/2MS+BA2mg/L+NAA 1mg/L, and pH 5.8, sugared 30g/L, AC2g/L, agar 6～8g/L; Condition of culture is: 20～30 ℃ of temperature, illumination: dark phase of 16h photophase/8h; Promptly obtain aseptic seedling after one month;
2) cutting of aseptic seedling: cut when the aseptic seedling bulb diameter that obtains reaches 3～5mm, the aseptic seedling after the cutting is continued inducing culture, inducing culture is: 1/2MS+BA 4mg/L+NAA1mg/L+KH
2PO
450～60mg/L+ Triiodobenzoic acid 0.5mg/L, pH6.6～6.8, sucrose 40～45g/L, AC 2g/L, agar 6～8g/L; Condition of culture is: 20～30 ℃ of temperature, illumination: dark phase of 16h photophase/8h; Obtain the aseptic seedlings of long 1cm;
3) seedling strong sprout: the aseptic seedlings to long 1cm is carried out strong seedling culture, and medium is 1/2MS+BA 4mg/L+NAA 1mg/L+KH
2P0
460mg/L+TIBA 0.5mg/L, pH 6.0～6.2, sucrose 50～60g/L, AC 2g/L, agar 6～8g/L; Condition of culture is: 20～30 ℃ of temperature, illumination: dark phase of 16h photophase/8h;
4) cutting again of aseptic seedling: when the aseptic seedling clove diameter after the strong seedling culture that obtains reaches 3～5mm, wherein a part of aseptic seedling is continued cutting, again the aseptic seedling after the cutting is carried out new inducing culture and strong seedling culture; So repeat constantly to obtain filial generation; The aseptic seedling that another part no longer cuts is transplanted seedlings;
5) transplant seedlings: will migrate in the vermiculite through the aseptic seedling of strong seedling culture and no longer cutting, keep the moisture abundance, treat the open country of transplanting seedlings once more when seedling leaves is grown to 5～7cm; When transplanting seedlings, environmental temperature is at 10～30 ℃, and avoids the sunlight direct projection.
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| CN200710046481XA CN101129131B (en) | 2007-09-27 | 2007-09-27 | Non-asepsis seedling re-cutting seedling-producing method of hippeastrum |
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| CN101869066B (en) * | 2009-04-24 | 2012-01-04 | 上海上房园林植物研究所 | Tissue culture method of African agapanthus |
| CN103493738B (en) * | 2013-10-14 | 2016-01-20 | 云南省农业科学院花卉研究所 | A kind of method of Hipeastrum vittalum (L Her.) Herb.-Amaryllisvittata Ait standardization cultured in vitro seedling |
| CN112690213B (en) * | 2021-01-14 | 2022-07-12 | 中国科学院华南植物园 | Tissue culture and rapid propagation method for high-quality seedlings of hippeastrum rutilum |
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