CN103098712A - Davallia mariesii breeding method - Google Patents
Davallia mariesii breeding method Download PDFInfo
- Publication number
- CN103098712A CN103098712A CN2013100488330A CN201310048833A CN103098712A CN 103098712 A CN103098712 A CN 103098712A CN 2013100488330 A CN2013100488330 A CN 2013100488330A CN 201310048833 A CN201310048833 A CN 201310048833A CN 103098712 A CN103098712 A CN 103098712A
- Authority
- CN
- China
- Prior art keywords
- seedling
- high mountain
- subculture
- condition
- under
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a Davallia mariesii breeding method, which comprises the following steps: an induction proliferation medium and a rooting medium are provided, the inducting and proliferation cultivating are concurrently carried out, after exercising seedling, and the seedling can be directly transplanted to a seedlings bag for culturing. The invention provides a technical scheme of a key link in a seedling breeding technology process of the Davallia mariesii, the proliferation rate is fast, the reproduction rates can reach 5-7 times during one period with 25-30 days; the rooting culture lasts about 20 days, each plant can breed 5-8 roots, the seedling is robust, the root develops, a tissue culture process is simplified, the cost and production time are greatly reduced, and the Davallia mariesii breeding method is suitable for standardization and industrial-scale production.
Description
Technical field
The invention belongs to the Floristry field, particularly the Propagtion of Pteridophyta Plants technical field.
Background technology
High mountain fern (Davallia mariesii) is the pteridophyte of Davalliaceae Davallia.Originate in Guatemala, enter China the nineties as cutting leaf.Be a kind of pteridophyte that cuts leaf that is suitable as, be widely used at present flower arrangement, the color of its leaf, leaf appearance in different poses and with different expressions and bule makes the people pleasing.Along with the development of decorative indoor plant, its product supply is in situation in short supply always.High mountain fern propagation method is mainly division propagation and sporogenesis, but division propagation quantity is few, and fragile plant type is recovered very slow and is subject to seasonal restrictions, and can not obtain in a short time a large amount of seedlings; Sporogenesis has affected reproduction speed because natural environment miospore planting percent is very low, and these method consumptions are large simultaneously, also are subject to the restriction in season, are difficult to carry out scale, standardized production and large-area popularization.Therefore the mating system of high mountain fern is imperative fast and effectively to explore one.And the advantage such as tissue culture technology has that reproduction speed is fast, but reproduction coefficient large, produce in the neat and consistent that the raises up seed anniversary, therefore utilize tissue culture technology to carry out the breeding of high mountain fern, can cultivate in a short time a large amount of seedlings, at present, also the high mountain fern not organized the report that training is bred.
Summary of the invention
The objective of the invention is to overcome existing high mountain fern sapling multiplication technology exists that material requirements is high, consumption large, is difficult to defective and the deficiency such as large-scale production; provide method and the special culture media of the suitable high mountain fern of a kind of energy industrialized tissue culture and rapid propagation, to satisfy the large-scale production needs of high mountain fern high quality seedling.
The special culture media that a kind of high mountain fern provided by the invention breeds is comprised of following two medium:
(1) induce proliferated culture medium:
The MS basic culture solution
(2) root media:
The 1/2MS basic culture solution
The special culture media that described high mountain fern breeds can also be comprised of following two medium:
(1) induce proliferated culture medium:
The MS basic culture solution
(2) root media:
The 1/2MS basic culture solution
The mating system of a kind of high mountain fern provided by the present invention comprises the following steps:
(1) after the high mountain fern spore of getting the maturing stage binds up with gauze, be 75% alcohol-pickled 30-40s with mass fraction under super-clean environment after, put into successively mass fraction and be 0.1% the mercuric chloride solution 6min that sterilizes, 10min sterilizes in the clorox mixed liquor, use again aseptic water washing 4～5 times, each 2min, namely obtain the aseptic spore of high mountain fern, described clorox mixed liquor is to add polysorbas20 to mix by the liquor natrii hypochloritis, the proportioning of liquor natrii hypochloritis and polysorbas20 is: mass fraction is to add 2 polysorbas20s in 2% liquor natrii hypochloritis 100ml,
(2) induce and breed cultivation: the aseptic spore access of high mountain fern that step (1) is obtained fills in following blake bottle of inducing proliferated culture medium:
The MS basic culture solution
Be the dark 15d～16d of cultivation under 25 ℃ ± 2 ℃ conditions in temperature, forwarding intensity of illumination to is 1500lx～2000lx, and temperature is 25 ℃ ± 2 ℃, and light application time is under the condition of 8～10h/d, continue to be cultured to the spore germination of high mountain fern and differentiate green spheroids, green spheroids is cut;
(3) subculture is cultivated: the green spheroids that step (2) is cut is transferred to fill with step (2) is described induces in the identical new blake bottle of inducing proliferated culture medium of proliferation culture medium formula, be 1500lx～2000lx in intensity of illumination, temperature is 25 ℃ ± 2 ℃, light application time is under the condition of 8～10h/d, after subculture is cultivated 25～30d, be transferred to again to fill with step (2) is described and induce in the identical new blake bottle of inducing proliferated culture medium of proliferation culture medium formula, and cultivating subculture cultivation 25～30d under described identical condition of culture with subculture, so repeatedly, wait breeding radix when reaching required quantity, changing the seedling of turning out over to step (4) cultivates, the green spheroids of seedling is not transferred to fill with step (2) is described and induces in the identical new blake bottle of inducing proliferated culture medium of proliferation culture medium formula, and cultivating continuation cultivation under described identical condition of culture with subculture, after seedling, change the seedling of turning out over to step (4) and cultivate,
(4) root media: the seedling that step (3) is obtained is inoculated in the blake bottle that fills following root media:
The 1/2MS basic culture solution
Be 1500lx～2000lx in intensity of illumination, temperature is 25 ℃ ± 2 ℃, and light application time is to be cultured to the plant base portion under the condition of 10～12h/d to send out roots;
(5) there is the blake bottle of root to be placed in step (4) plant length and carries out conventional hardening 3～5d in booth, again the seedling in blake bottle is taken out, clean routinely the medium on seedling, putting into mass fraction and be 0.1～0.2% carbendazim solution sterilizes after 1～2min, transplant to the Seedling bag that following matrix is housed, described matrix is by peat, detritus soil, perlite and vermiculite mix, peat, detritus soil, the mass ratio of perlite and vermiculite is: peat: detritus soil: perlite: vermiculite=7:3:1:0.5, routine water and the fertilizing management condition under, after growth 40d, namely get high mountain fern transplanted seedling.
The mating system of described high mountain fern, can also carry out by the following method in step (2), step (3) and step (4):
Step (2) is induced and is bred that to cultivate be that the aseptic spore access of high mountain fern that step (1) obtains is filled in following blake bottle of inducing proliferated culture medium:
The MS basic culture solution
Be the dark 16d of cultivation under 25 ℃ of conditions in temperature, forwarding intensity of illumination to is 1800lx, and temperature is 25 ℃, and light application time is under the condition of 9h/d, continues to be cultured to the spore germination of high mountain fern and differentiates green spheroids, and green spheroids is cut;
The condition that step (3) subculture is cultivated is that intensity of illumination is 1800lx, and temperature is 25 ℃, and light application time is 9h/d, and subculture is cultivated 27d.
Step (4) root media is that the seedling that step (3) obtains is inoculated in the blake bottle that fills following root media:
The 1/2MS basic culture solution
Be 1800lx in intensity of illumination, temperature is 25 ℃, and light application time is to be cultured to the plant base portion under the condition of 11h/d to send out roots.
The present invention has following advantages and effect:
1, the high mountain fern of the present invention special culture media of breeding and the mating system of high mountain fern, the group that has configured suitable especially high mountain fern is trained special culture media and condition of culture and the method for breeding, adopt this special culture media and method reproduction speed fast, 25～30 days is one-period, and the one-period breeding rate can reach 5～7 times; About culture of rootage 20 days, every strain plant can grow 5～8 roots, and its seedling is healthy and strong, and well developed root system is grown after being beneficial to bottle outlet.
2, the present invention has proposed the technical scheme to key link in whole high mountain fern seedling production technology flow process first, synchronously carrying out bud induces and breeds cultivation, subculture medium is induced with proliferated culture medium identical with bud, condition of culture is basic identical, not only reach good effect but also simplified the cultivation program of tissue culture technology, cost and production time have greatly been saved, also solved the seedling parental source complexity that conventional breeding of method is produced, the unsettled difficult problem of seedling quality, the inventive method, can make kind of seedlings stable, source of seedling is single, suitable standardization, the batch production large-scale production, the good seed of unified standard is provided for market.
3, the sterile rootage seedling direct transplantation produced of the inventive method enters in Seedling bag and manages, and has saved the link that group training seedling nutritive cube is transplanted, and the seedling of transplant survival is convenient to transportation, and the transplanted seedling strong adaptability can directly be planted after plucking Seedling bag.
4, the mating system of the present invention anniversary of can realizing in culturing room produces, and has both saved land resources, has improved again economic benefit, has overcome the difficult point that high mountain fern seedling can't be produced in the anniversary.
Embodiment
Embodiment 1
(1) get the high mountain fern spore in maturing stage, after binding up with gauze, be 75% alcohol-pickled 30s with mass fraction under super-clean environment after, put into successively mass fraction and be 0.1% the mercuric chloride solution 6min that sterilizes, 10min sterilizes in the clorox mixed liquor, use again aseptic water washing 4～5 times, each 2min, namely obtain the aseptic spore of high mountain fern, described clorox mixed liquor is to add polysorbas20 to mix by the liquor natrii hypochloritis, the proportioning of liquor natrii hypochloritis and polysorbas20 is: mass fraction is to add 2 polysorbas20s in 2% liquor natrii hypochloritis 100ml.
(2) induce and breed cultivation: the aseptic spore access of high mountain fern that step (1) is obtained fills in following blake bottle of inducing proliferated culture medium:
The MS basic culture solution
Be the dark 15d of cultivation under 23 ℃ of conditions in temperature, forwarding intensity of illumination to is 2000lx, and temperature is 23 ℃, and light application time is under the condition of 8h/d, continues to be cultured to the spore germination of high mountain fern and differentiates green spheroids, and green spheroids is cut.
(3) subculture is cultivated: the green spheroids that step (2) is cut is transferred to fill with step (2) is described induces in the identical new blake bottle of inducing proliferated culture medium of proliferation culture medium formula, be 2000lx in intensity of illumination, temperature is 23 ℃, light application time is under the condition of 8h/d, after subculture is cultivated 25d, be transferred to again to fill with step (2) is described and induce in the identical new blake bottle of inducing proliferated culture medium of proliferation culture medium formula, and cultivating subculture cultivation under described identical condition of culture with subculture, that is: be 2000lx in intensity of illumination, temperature is 23 ℃, light application time is under the condition of 8h/d, subculture is cultivated 25d, so repeatedly, wait breeding radix when reaching required quantity, changing the seedling of turning out over to step (4) cultivates.The number of times that subculture is cultivated can group as required be trained the quantity of seedling and decide, and can cultivate once by a subculture, also can cultivate more than 2 times by subculture.
The green spheroids of seedling is not transferred to fill with step (2) is described and induces in the identical new blake bottle of inducing proliferated culture medium of proliferation culture medium formula, and cultivating subculture cultivation under described identical condition of culture with subculture, that is: be 2000lx in intensity of illumination, temperature is 23 ℃, light application time is to continue under the condition of 8h/d to cultivate, after seedling, cultivate changing step (4) after the seedling of turning out over to.
(4) root media: the seedling that step (3) is obtained is inoculated in the blake bottle that fills following root media:
The 1/2MS basic culture solution
Be 2000lx in intensity of illumination, temperature is 23 ℃, and light application time is to be cultured to the plant base portion under the condition of 10h/d to send out roots;
(5) there is the blake bottle of root to be placed under booth inscattering light step (4) plant length and carries out conventional hardening 3d, again the seedling in blake bottle is taken out, clean routinely the medium on seedling, putting into mass fraction and be 0.1% carbendazim solution sterilizes after 1～2min, transplant to the Seedling bag that matrix is housed, and with this Seedling bag be placed in light transmittance be cultivate under 30% sunshade net, water every day in vegetative period moistening to matrix and carry out the blade face water spray and drip to the blade face, it is moistening to matrix that 3 days winter dormancy phases were watered a water; Mass fraction is executed in transplantation of seedlings to the Seedling bag after growth 10-12d be 1% composite fertilizer 2 times, and each fertilising irrigates matrix, 2 fertilising interval 10d, and growth 40d namely gets high mountain fern transplanted seedling; Described matrix is mixed by peat, detritus soil, perlite and vermiculite, and the mass ratio of peat, detritus soil, perlite and vermiculite is: peat: detritus soil: perlite: vermiculite=7:3:1:0.5; N, P in described composite fertilizer
2O
5, K
2The mass ratio of O is: N:P
2O
5: K
2O=20:10:10.
Embodiment 2
Embodiment 2 all the other measures except following measure difference are identical with embodiment 1, repeat no more.(1) after the high mountain fern spore of getting the maturing stage binds up with gauze, be 75% alcohol-pickled 35s with mass fraction under super-clean environment.
(2) induce and the proliferated culture medium of inducing of breeding cultivation is:
The MS basic culture solution
Be the dark 16d of cultivation under 25 ℃ of conditions in temperature, forwarding intensity of illumination to is 1800lx, and temperature is 25 ℃, and light application time is under the condition of 9h/d, continues to be cultured to the spore germination of high mountain fern and differentiates green spheroids, and green spheroids is cut.
(3) subculture is cultivated: each subculture is cultivated 28d, cultivated 3 times for subculture, the condition that subculture is cultivated is: intensity of illumination is 1800lx, temperature is 25 ℃, light application time is 9h/d, subculture is cultivated 27d, and the medium that subculture is cultivated is identical with the described proliferation culture medium formula of inducing of step (2).
(4) root media is:
The 1/2MS basic culture solution
Be 1800lx in intensity of illumination, temperature is 25 ℃, and light application time is to be cultured to the plant base portion under the condition of 11h/d to send out roots;
(5) blake bottle is placed in and carries out conventional hardening 4d in booth, then the seedling in blake bottle is taken out, and cleaning and putting into mass fraction after the medium on seedling is after 0.2% carbendazim solution sterilization 2min, to transplant to Seedling bag and cultivate.
Embodiment 3
Embodiment 3 all the other measures except following measure difference are identical with embodiment 1, repeat no more.
(1) after the high mountain fern spore of getting the maturing stage binds up with gauze, be 75% alcohol-pickled 40s with mass fraction under super-clean environment.
(2) induce proliferated culture medium to be:
The MS basic culture solution
Be the dark 15d of cultivation under 27 ℃ of conditions in temperature, forwarding intensity of illumination to is 1500lx, and temperature is 27 ℃, and light application time is under the condition of 10h/d, continues to be cultured to the spore germination of high mountain fern and differentiates green spheroids, and green spheroids is cut.
(3) subculture is cultivated: each subculture is cultivated 30d, and subculture has been cultivated 5 times altogether, and the subculture condition of culture is: intensity of illumination is 1500lx, temperature is 27 ℃, light application time is 10h/d, and subculture is cultivated 30d, and the medium that subculture is cultivated is identical with the described proliferation culture medium formula of inducing of step (2).
(4) root media is:
The 1/2MS basic culture solution
Be 1500lx in intensity of illumination, temperature is 27 ℃, and light application time is to be cultured to the plant base portion under the condition of 12h/d to send out roots;
(5) blake bottle is placed in and carries out conventional hardening 5d in booth, then the seedling in blake bottle is taken out, and cleaning and putting into mass fraction after the medium on seedling is after 0.2% carbendazim solution sterilization 1.5min, to transplant to Seedling bag and cultivate.
Above each embodiment reproduction speed is fast, and 25～30 days is a breeding cycle, and the one-period breeding rate can reach 5～7 times; About culture of rootage 20 days, every strain plant can grow 5～8 roots, and its seedling is healthy and strong, and well developed root system is grown after being beneficial to bottle outlet.
Claims (4)
1. the special culture media that the high mountain fern breeds, is characterized in that, is comprised of following two medium:
(1) induce proliferated culture medium:
The MS basic culture solution
(2) root media:
The 1/2MS basic culture solution
2. the high mountain fern according to claim 1 special culture media of breeding, is characterized in that, is comprised of following two medium:
(1) induce proliferated culture medium:
The MS basic culture solution
(2) root media:
The 1/2MS basic culture solution
3. the mating system of a high mountain fern comprises the following steps:
(1) after the high mountain fern spore of getting the maturing stage binds up with gauze, be 75% alcohol-pickled 30-40s with mass fraction under super-clean environment after, put into successively mass fraction and be 0.1% the mercuric chloride solution 6min that sterilizes, 10min sterilizes in the clorox mixed liquor, use again aseptic water washing 4～5 times, each 2min, namely obtain the aseptic spore of high mountain fern, described clorox mixed liquor is to add polysorbas20 to mix by the liquor natrii hypochloritis, the proportioning of liquor natrii hypochloritis and polysorbas20 is: mass fraction is to add 2 polysorbas20s in 2% liquor natrii hypochloritis 100ml,
(2) induce and breed cultivation: the aseptic spore access of high mountain fern that step (1) is obtained fills in following blake bottle of inducing proliferated culture medium:
The MS basic culture solution
Be the dark 15d～16d of cultivation under 25 ℃ ± 2 ℃ conditions in temperature, forwarding intensity of illumination to is 1500lx～2000lx, and temperature is 25 ℃ ± 2 ℃, and light application time is under the condition of 8～10h/d, continue to be cultured to the spore germination of high mountain fern and differentiate green spheroids, green spheroids is cut;
(3) subculture is cultivated: the green spheroids that step (2) is cut is transferred to fill with step (2) is described induces in the identical new blake bottle of inducing proliferated culture medium of proliferation culture medium formula, be 1500lx～2000lx in intensity of illumination, temperature is 25 ℃ ± 2 ℃, light application time is under the condition of 8～10h/d, after subculture is cultivated 25～30d, be transferred to again to fill with step (2) is described and induce in the identical new blake bottle of inducing proliferated culture medium of proliferation culture medium formula, and cultivating subculture cultivation 25～30d under described identical condition of culture with subculture, so repeatedly, wait breeding radix when reaching required quantity, changing the seedling of turning out over to step (4) cultivates, the green spheroids of seedling is not transferred to fill with step (2) is described and induces in the identical new blake bottle of inducing proliferated culture medium of proliferation culture medium formula, and cultivating continuation cultivation under described identical condition of culture with subculture, after seedling, change the seedling of turning out over to step (4) and cultivate,
(4) root media: the seedling that step (3) is obtained is inoculated in the blake bottle that fills following root media:
The 1/2MS basic culture solution
Be 1500lx～2000lx in intensity of illumination, temperature is 25 ℃ ± 2 ℃, and light application time is to be cultured to the plant base portion under the condition of 10～12h/d to send out roots;
(5) there is the blake bottle of root to be placed in step (4) plant length and carries out conventional hardening 3～5d in booth, again the seedling in blake bottle is taken out, clean routinely the medium on seedling, putting into mass fraction and be 0.1～0.2% carbendazim solution sterilizes after 1～2min, transplant to the Seedling bag that following matrix is housed, described matrix is by peat, detritus soil, perlite and vermiculite mix, peat, detritus soil, the mass ratio of perlite and vermiculite is: peat: detritus soil: perlite: vermiculite=7:3:1:0.5, routine water and the fertilizing management condition under, after growth 40d, namely get high mountain fern transplanted seedling.
4. the mating system of high mountain fern according to claim 3 is characterized in that:
Step (2) is induced and is bred that to cultivate be that the aseptic spore access of high mountain fern that step (1) obtains is filled in following blake bottle of inducing proliferated culture medium:
The MS basic culture solution
Be the dark 16d of cultivation under 25 ℃ of conditions in temperature, forwarding intensity of illumination to is 1800lx, and temperature is 25 ℃, and light application time is under the condition of 9h/d, continues to be cultured to the spore germination of high mountain fern and differentiates green spheroids, and green spheroids is cut;
The condition that step (3) subculture is cultivated is that intensity of illumination is 1800lx, and temperature is 25 ℃, and light application time is 9h/d, and subculture is cultivated 27d;
Step (4) root media is that the seedling that step (3) obtains is inoculated in the blake bottle that fills following root media:
The 1/2MS basic culture solution
Be 1800lx in intensity of illumination, temperature is 25 ℃, and light application time is to be cultured to the plant base portion under the condition of 11h/d to send out roots.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310048833.0A CN103098712B (en) | 2013-02-07 | 2013-02-07 | Davallia mariesii breeding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310048833.0A CN103098712B (en) | 2013-02-07 | 2013-02-07 | Davallia mariesii breeding method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103098712A true CN103098712A (en) | 2013-05-15 |
| CN103098712B CN103098712B (en) | 2014-03-26 |
Family
ID=48307172
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310048833.0A Expired - Fee Related CN103098712B (en) | 2013-02-07 | 2013-02-07 | Davallia mariesii breeding method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103098712B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104206279A (en) * | 2014-09-21 | 2014-12-17 | 云南集创园艺科技有限公司 | Induction medium for green spherical bodies of hemionitis arifolia |
| CN104206280A (en) * | 2014-09-21 | 2014-12-17 | 云南省农业科学院花卉研究所 | Tissue culture and rapid propagation method of hemionitis arifolia seedlings by virtue of green spherical body manner |
| CN109089882A (en) * | 2018-08-30 | 2018-12-28 | 云南省农业科学院花卉研究所 | A kind of moss tissue culture directly induced by spore and seedling culture method |
| CN109874672A (en) * | 2019-03-28 | 2019-06-14 | 北京市大东流苗圃 | A kind of spherosome method for tissue culture of tuber fern seedling |
| CN110663548A (en) * | 2019-09-24 | 2020-01-10 | 福建省农业科学院作物研究所 | Aseptic germination seedling raising method for platycerium wallichii spores |
| CN115211371A (en) * | 2022-08-23 | 2022-10-21 | 桂林亦元生现代生物技术有限公司 | Method for tissue culture and breeding of drynaria |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| USPP9113P (en) * | 1993-12-13 | 1995-04-18 | Jack K. Austin | Newbold's Diamond Leatherleaf Fern |
| US5650551A (en) * | 1993-12-13 | 1997-07-22 | Jack K. Austin | Newbold's diamond, a densely foliolate, complanate leatherleaf fern |
| CN1726760A (en) * | 2005-06-08 | 2006-02-01 | 中国科学院武汉植物园 | The artificial fecundation method of Mongolian oak fern |
| CN1868262A (en) * | 2006-06-20 | 2006-11-29 | 慈溪市蔬菜开发有限公司 | Tissue culture seedling-growing method for bird's-net fern |
| CN100407902C (en) * | 2004-12-13 | 2008-08-06 | 中国科学院植物研究所 | Drynaria fortunei breeding method |
| CN102119656A (en) * | 2010-11-10 | 2011-07-13 | 天津滨海国际花卉科技园区股份有限公司 | Bird nest fern spore germination breeding method |
| CN102715092A (en) * | 2012-07-06 | 2012-10-10 | 中国热带农业科学院热带作物品种资源研究所 | Method for rapidly reproducing new pteris fern seedlings by using prothallium reproduction approaches |
-
2013
- 2013-02-07 CN CN201310048833.0A patent/CN103098712B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| USPP9113P (en) * | 1993-12-13 | 1995-04-18 | Jack K. Austin | Newbold's Diamond Leatherleaf Fern |
| US5650551A (en) * | 1993-12-13 | 1997-07-22 | Jack K. Austin | Newbold's diamond, a densely foliolate, complanate leatherleaf fern |
| CN100407902C (en) * | 2004-12-13 | 2008-08-06 | 中国科学院植物研究所 | Drynaria fortunei breeding method |
| CN1726760A (en) * | 2005-06-08 | 2006-02-01 | 中国科学院武汉植物园 | The artificial fecundation method of Mongolian oak fern |
| CN1868262A (en) * | 2006-06-20 | 2006-11-29 | 慈溪市蔬菜开发有限公司 | Tissue culture seedling-growing method for bird's-net fern |
| CN102119656A (en) * | 2010-11-10 | 2011-07-13 | 天津滨海国际花卉科技园区股份有限公司 | Bird nest fern spore germination breeding method |
| CN102715092A (en) * | 2012-07-06 | 2012-10-10 | 中国热带农业科学院热带作物品种资源研究所 | Method for rapidly reproducing new pteris fern seedlings by using prothallium reproduction approaches |
Non-Patent Citations (1)
| Title |
|---|
| 文伟: "骨碎补愈伤组织的诱导及无性系建立的研究", 《黑龙江农业科学》, no. 2, 31 December 2010 (2010-12-31) * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104206279A (en) * | 2014-09-21 | 2014-12-17 | 云南集创园艺科技有限公司 | Induction medium for green spherical bodies of hemionitis arifolia |
| CN104206280A (en) * | 2014-09-21 | 2014-12-17 | 云南省农业科学院花卉研究所 | Tissue culture and rapid propagation method of hemionitis arifolia seedlings by virtue of green spherical body manner |
| CN109089882A (en) * | 2018-08-30 | 2018-12-28 | 云南省农业科学院花卉研究所 | A kind of moss tissue culture directly induced by spore and seedling culture method |
| CN109874672A (en) * | 2019-03-28 | 2019-06-14 | 北京市大东流苗圃 | A kind of spherosome method for tissue culture of tuber fern seedling |
| CN110663548A (en) * | 2019-09-24 | 2020-01-10 | 福建省农业科学院作物研究所 | Aseptic germination seedling raising method for platycerium wallichii spores |
| CN110663548B (en) * | 2019-09-24 | 2021-05-04 | 福建省农业科学院作物研究所 | Aseptic germination seedling raising method for Platycerium setosum wallichii hook |
| CN115211371A (en) * | 2022-08-23 | 2022-10-21 | 桂林亦元生现代生物技术有限公司 | Method for tissue culture and breeding of drynaria |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103098712B (en) | 2014-03-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102499090B (en) | Method for isolated culture of Haworthia succulent plants | |
| CN102187810B (en) | Tissue culture propagation method for curcuma soloensis | |
| CN101803515A (en) | Method for rapidly growing and cultivating dendrobium officinale | |
| CN102301951B (en) | Method for rapidly propagating roots of subprostrate sophora by tissue culture | |
| CN103098712B (en) | Davallia mariesii breeding method | |
| CN102823497B (en) | Clonal tissue culture breeding method of Liquidambar formosana hance | |
| CN103734014B (en) | A kind of quick breeding method for tissue culture of anisetree bark | |
| CN101707979A (en) | Sugarcane tissue-culture-seedling bare-seedling field-transplanting method | |
| CN103348920B (en) | Rapid propagation method for high quality seedlings of Kyara | |
| CN103283453A (en) | Large-scale cutting propagation method of ligustrum japonicum 'howardii' | |
| CN107018896B (en) | A kind of method of facility cuttage tilia miqueliana | |
| CN105123529A (en) | Rapid propagation and efficient cultivation method of Bletilla striata | |
| CN103125386B (en) | Industrial horseradish planting method | |
| CN101926259A (en) | Orchid germchit propagating method | |
| CN104585037A (en) | Tissue culture rapid-propagation method of beaucarnea recurvata | |
| CN103988777A (en) | Lobule dwarf type magnolia grandiflora tender stem segment isolated culture method | |
| CN103168692B (en) | Salix saposhnikovii tissue culture method | |
| CN104920223A (en) | Chinese cymbidium seedling breeding method | |
| CN104770173B (en) | High-efficiency seedling cultivation method for promoting premature tillering of sugarcane tissue cultured seedlings | |
| CN109349105A (en) | A kind of iris tissue-cultured seedling mating system | |
| CN102893872A (en) | Tissue culture method for domesticated seedlings of iris pallida | |
| CN101822211A (en) | Tissue cultivating method of dendrobium officinale growing in Yunnan | |
| CN101743908A (en) | Tissue culture, rapid propagation and cultivation method of grevillea banksii | |
| CN103947434A (en) | Method of planting wild soybeans on saline-alkali soil | |
| CN103430850B (en) | Tissue culture method for polyploid hemerocallis middendorfii and culture medium |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140326 Termination date: 20160207 |