CN103798123B - A kind of half leaf porphyra breeding method - Google Patents
A kind of half leaf porphyra breeding method Download PDFInfo
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- CN103798123B CN103798123B CN201410056088.9A CN201410056088A CN103798123B CN 103798123 B CN103798123 B CN 103798123B CN 201410056088 A CN201410056088 A CN 201410056088A CN 103798123 B CN103798123 B CN 103798123B
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Abstract
The invention discloses a kind of half leaf porphyra breeding method, described method comprises the steps: acquisition half leaf laver nutritive algal filament; Half leaf laver nutritive algal filament is cultivated in culture fluid, until form the filamentous with the sporangial branchlets of setting ratio, condition of culture is: temperature is 20-30 DEG C, and light intensity is 1000-2600lx, periodicity of illumination is 10-16h light application time, and culture fluid sea water specific gravity is 1.026-1.031; The above-mentioned filamentous with the sporangial branchlets of setting ratio is placed on cling matrix, stimulation by running water, until form the conchospore meeting demand density of collecting seedling, completes the nursery of half leaf laver.Thus, solve prior art cannot double leaf laver carry out fast, the problem of high efficiency seedling cultivating.
Description
Technical field
The invention belongs to technical field of aquaculture, specifically, relate to a kind of porphyra breeding method, more particularly, relate to a kind of half leaf porphyra breeding method.
Background technology
Laver is the tangleweed that a kind of nutrition and economic worth are all very high, and Porphyra kind is more, but China cultivar only has Porphyra haitanensis and porphyra yezoensis at present.Porphyra haitanensis is high-temperature adaptability kind, mainly in Fujian, Zhejiang two province is coastal cultivates, porphyra yezoensis is then low temperature adaptability kind, mainly cultivates on a large scale in Jiangsu Province.Porphyra haitanensis and porphyra yezoensis are intertidal zone kind, need periodically dry dew and Exposure to Sunlight, to remove the assorted algae such as Enteromorpha, diatom and disease, so, require higher to the natural conditions in cultivation sea area.Therefore, can only in intertidal zone, shoal utilizes semi-floating raft type to cultivate, or utilizes bamboo pole to carry out inserted-link type cultivation in offshore deeper water.And the length of bamboo pole is limited, cause being difficult to expand to deeper water.In recent years, the cultivation scale of Porphyra haitanensis and porphyra yezoensis reached threshold value, was difficult to expand again.
And along with people are to the intensification of seaweed products nutritive value and green safety cognition, the market of seaweed products is significantly expanded, and the demand of market to laver is increasing, there is the phenomenon that supply falls short of demand.The planting technique that vast laver culture family active demand is new and new cultivar, to expand laver area under cultivation, increase laver output, to improve economic well-being of workers and staff.
Half leaf laver is China's distinctive kind in Huanghai-Bohai seas district, primary growth at the low-lying place of intertidal zone, even if still live in water when spring low water.By to its ecological observation in natural waters, find that this kind anti-attachment ability is comparatively strong, the growth busy season, frond color and luster was comparatively dark, and body is larger in January to March.As can be seen here, half leaf laver is the kind of suitable cultivation.If seedling growing process is ripe, be expected to carry out the cultivation of raft formula in comparatively abysmal area, to make up the restriction by shoal cultivation of Porphyra haitanensis and porphyra yezoensis.
Do not need to experience to do, be exposed to the sun the stage in the natural life cycle of half leaf laver, and, by studying its stress physiology, find that half leaf laver can not tolerate strong dehydration, freezing process, so, the characteristic of half leaf laver and porphyra yezoensis and Porphyra haitanensis difference larger.Test shows, to temperature, illumination, photoperiodic control method when adopting existing porphyra yezoensis, Porphyra haitanensis nursery, half leaf conchocelis of porphyra cannot form a large amount of sporangial branchlets, and therefore, existing porphyra breeding method is also not suitable for the nursery of half leaf laver.At present, not yet occur that double leaf laver carries out the method for high efficiency seedling cultivating, thus limit the cultivation of half leaf laver.
Summary of the invention
The object of this invention is to provide a kind of half leaf porphyra breeding method, with solve prior art cannot double leaf laver carry out fast, the problem of high efficiency seedling cultivating.
For solving the problems of the technologies described above, the present invention adopts following technical proposals to be achieved:
A kind of half leaf porphyra breeding method, described method comprises the steps:
Obtain half leaf laver nutritive algal filament;
Half leaf laver nutritive algal filament is cultivated in culture fluid, until form the filamentous with the sporangial branchlets of setting ratio, condition of culture is: temperature is 20-30 DEG C, and light intensity is 1000-2600lx, periodicity of illumination is 10-16h light application time, and culture fluid sea water specific gravity is 1.026-1.031;
The above-mentioned filamentous with the sporangial branchlets of setting ratio is placed on cling matrix, stimulation by running water, until form the conchospore meeting demand density of collecting seedling, completes the nursery of half leaf laver.
Half leaf porphyra breeding method as above, when cultivating half leaf laver nutritive algal filament in culture fluid, changes one time of nutrition liquid at regular intervals.
Half leaf porphyra breeding method as above, described half leaf laver nutritive algal filament adopts following method to obtain:
Gather half leaf thallus of porphyra, cleaning, cultivation, gain freedom filamentous;
In culture fluid, cultivate free conchocelis, cultivate a period of time, obtain half leaf laver nutritive algal filament, condition of culture is: temperature is 15-22 DEG C, and light intensity is 500-3000lx, and periodicity of illumination is 8-16h light application time, and culture fluid sea water specific gravity is 1.016-1.024.
Half leaf porphyra breeding method as above, the culture fluid of described cultivation nutrition algal filament and the culture fluid of cultivation free conchocelis all preferably add NaNO
3and K
2hPO
3sterilization seawater.
Compared with prior art, advantage of the present invention and good effect are: the present invention is when cultivation half leaf laver nutritive algal filament, by controlling sea water specific gravity, in conjunction with suitable temperature, light intensity and periodicity of illumination, a large amount of cystites and sporangial branchlets can be formed fast, and then achieve quick, the high efficiency seedling cultivating of half leaf laver, be conducive to the large-scale farming realizing half leaf laver.
After reading the specific embodiment of the present invention by reference to the accompanying drawings, the other features and advantages of the invention will become clearly.
Accompanying drawing explanation
Fig. 1 is the flow chart of the present invention half leaf porphyra breeding method first embodiment;
Fig. 2 to Fig. 5 is the microscopical examination showed intention of half leaf laver nutritive algal filament under different incubation time in Fig. 1 embodiment;
Fig. 6 is the flow chart of another embodiment of the present invention half leaf porphyra breeding method.
Embodiment
Below in conjunction with the drawings and specific embodiments, technical scheme of the present invention is described in further detail.
Refer to Fig. 1, this figure is depicted as the flow chart of the present invention half leaf porphyra breeding method first embodiment.
As shown in Figure 1, this embodiment double leaf laver process of carrying out nursery is specific as follows:
Step 11: obtain the half leaf laver nutritive algal filament preserved.
Half leaf laver nutritive algal filament is generally kept in culture fluid, needs control temperature, illumination and photoperiod in preservation process, to maintain nutrition algal filament state.
Step 12: cultivate nutrition algal filament in culture fluid, controls condition of culture, until form the filamentous with the sporangial branchlets of setting ratio.
It is in the culture fluid of 1.026-1.031 that half leaf laver nutritive algal filament is transferred to sea water specific gravity, and control cultivation temperature is 20-30 DEG C, and light intensity is 1000-2600lx, and periodicity of illumination is 10-16h light application time, cultivates.Wherein, culture fluid preferably adds NaNO
3and K
2hPO
3sterilization seawater, and the sea water specific gravity of culture fluid can by adding appropriate fresh water or NaCl regulates.Preferred condition of culture is: temperature is 20-22 DEG C, and light intensity is 1500-2000lx, and periodicity of illumination is 12h light application time, and sea water specific gravity is 1.026.In incubation, within one week, change one time of nutrition liquid, cultivate two months, the thread scale of construction increases to more than 6 times, and more than 70% forms sporangial branchlets, meets requirement of collecting seedling.Under different incubation time, the microscopical examination showed intention of filamentous can referring to figs. 2 to shown in Fig. 5.
Be respectively shown in Fig. 2 to Fig. 5 nutrition algal filament cultivate 2 weeks, 4 weeks, 7 weeks, two months time half leaf conchocelis of porphyra microscopical examination showed be intended to.As shown in Figure 2, be cultivate nutrition algal filament in the culture fluid of 1.026 at sea water specific gravity, after cultivating 2 weeks, half leaf conchocelis of porphyra starts formation and expands (as arrow indication in Fig. 2).Continue cultivation 4 weeks, as shown in Figure 3, half leaf conchocelis of porphyra is formed and expands (as arrow indication in Fig. 3) in a large number.Continue cultivation 7 weeks, as shown in Figure 4, half leaf conchocelis of porphyra forms a large amount of sporangial branchlets, and the new sporangial branchlets formed is made up of (as arrow indication in Fig. 4) 1-3 cell.Continue to be cultured to two months, as shown in Figure 5, half leaf conchocelis of porphyra more than 70% forms sporangial branchlets, and sporangial branchlets is by multiple cellularity.Thus illustrate that the seedling-cultivating method of this embodiment can obtain a high proportion of sporangial branchlets at short notice, also, the seedling-cultivating method of this embodiment is practicable.
Step 13: be placed on cling matrix by the above-mentioned filamentous with sporangial branchlets, stimulation by running water, forms satisfied collecting seedling and requires the conchospore of density.
Prepare large glass box, separate with 1 layer of 300 mesh sieve thin,tough silk bottom the glass box, the filamentous with more than 70% sporangial branchlets is placed on as on the bolting silk of cling matrix, with the stimulation by running water three days of water temperature 18-19.5 DEG C, get bolting silk microscopy to observe, conchospore attachment density on average reaches 30/cm
2, meet required conchospore density of collecting seedling, complete the nursery of half leaf laver.
After this, cling matrix can be moved to deep-sea breeding district together with the conchospore of attachment on it to cultivate.
This embodiment gathers half leaf laver nutritive algal filament under preservation state, moved in the culture fluid of specific sea water specific gravity, coordinate suitable temperature, light intensity and periodicity of illumination, a large amount of cystites and sporangial branchlets can be formed fast, and then achieve quick, the high efficiency seedling cultivating of half leaf laver, be conducive to the large-scale farming realizing half leaf laver.
Refer to Fig. 6, the figure shows the flow chart of the present invention half leaf porphyra breeding method second embodiment.
As shown in Figure 6, this embodiment double leaf laver process of carrying out nursery is specific as follows:
Step 21: gather half leaf thallus of porphyra, cleaning, cultivation, gain freedom filamentous.
From half leaf laver select vegetatively bright-colored, glossiness good, individuality is large, half leaf thallus of porphyra of healthy growth, thallus unfolded, brush away the attachments such as the mud scum on thallus surface, assorted algae with banister bruss, clean 3 times with sterilization seawater.Then, be placed in nutrient solution and cultivate, until the filamentous that gains freedom.
Step 22: cultivate free conchocelis in culture fluid, controls condition of culture, obtains half leaf laver nutritive algal filament.
Chosen by free conchocelis in step 11, being placed on sea water specific gravity is in the culture fluid of 1.016-1.024, and control cultivation temperature is 15-22 DEG C, and light intensity is 500-3000lx, and periodicity of illumination is 8-16h light application time, cultivates.Wherein, culture fluid preferably adds NaNO
3and K
2hPO
3sterilization seawater, and the sea water specific gravity of culture fluid can by adding appropriate fresh water or NaCl regulates.Preferred condition of culture is: temperature is 20 DEG C, and light intensity is 2500lx, and periodicity of illumination is 12h light application time, and sea water specific gravity is 1.018-1.022.In incubation, within one week, change one time of nutrition liquid, cultivate one month, obtain enough nutrition algal filaments.
Step 23: cultivate nutrition algal filament in culture fluid, controls condition of culture, until form the filamentous with the sporangial branchlets of setting ratio.
Sea water specific gravity transferred to by half leaf laver nutritive algal filament step 22 obtained is in the culture fluid of 1.026-1.031, and control cultivation temperature is 20-30 DEG C, and light intensity is 1000-2600lx, and periodicity of illumination is 10-16h light application time, cultivates.Wherein, culture fluid preferably adds NaNO
3and K
2hPO
3sterilization seawater, and the sea water specific gravity of culture fluid can by adding appropriate fresh water or NaCl regulates.Preferred condition of culture is: temperature is 25-28 DEG C, and light intensity is 2000-2600lx, and periodicity of illumination is 14h light application time, and sea water specific gravity is 1.031.In incubation, within one week, change one time of nutrition liquid, cultivate two months, filars lengths increases to more than 5 times, and more than 85% forms sporangial branchlets, meets requirement of collecting seedling.
Step 24: be placed on cling matrix by the above-mentioned filamentous with sporangial branchlets, stimulation by running water, forms satisfied collecting seedling and requires the conchospore of density.
Preparing plastic containing box, tiling at the bottom of case is collected seedling with nettle, the filamentous with more than 85% sporangial branchlets is placed on as on the nettle of cling matrix, with the stimulation by running water three days of water temperature 23-25 DEG C, get nettle microscopy to observe, conchospore attachment density on average reaches 25/cm
2, meet required conchospore density of collecting seedling, complete the nursery of half leaf laver.
After this, cling matrix can be moved to deep-sea breeding district together with the conchospore of attachment on it to cultivate.
This embodiment directly gathers half leaf thallus of porphyra, first turn out free conchocelis, then under specific sea water specific gravity, nutrition algal filament is turned out again, thereafter, nutrition algal filament being moved to sea water specific gravity is greater than in the culture fluid of free conchocelis place culture fluid again, coordinates suitable temperature, light intensity and periodicity of illumination, can form a large amount of cystites and sporangial branchlets fast, and then achieve quick, the high efficiency seedling cultivating of half leaf laver, be conducive to the large-scale farming realizing half leaf laver.
Above embodiment only in order to technical scheme of the present invention to be described, but not is limited; Although with reference to previous embodiment to invention has been detailed description, for the person of ordinary skill of the art, still can modify to the technical scheme described in previous embodiment, or equivalent replacement is carried out to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of the present invention's technical scheme required for protection.
Claims (3)
1. a half leaf porphyra breeding method, is characterized in that, described method comprises the steps:
Gather half leaf thallus of porphyra, cleaning, cultivation, gain freedom filamentous;
In culture fluid, cultivate free conchocelis, cultivate one month, obtain half leaf laver nutritive algal filament, condition of culture is: temperature is 15-22 DEG C, and light intensity is 500-3000lx, and periodicity of illumination is 8-16h light application time, and culture fluid sea water specific gravity is 1.018;
Half leaf laver nutritive algal filament is cultivated in culture fluid, until form the filamentous with the sporangial branchlets of setting ratio, condition of culture is: temperature is 20-30 DEG C, and light intensity is 1000-2600lx, periodicity of illumination is 10-16h light application time, and culture fluid sea water specific gravity is 1.031;
The above-mentioned filamentous with the sporangial branchlets of setting ratio is placed on cling matrix, stimulation by running water, until form the conchospore meeting demand density of collecting seedling, completes the nursery of half leaf laver.
2. half leaf porphyra breeding method according to claim 1, is characterized in that, when cultivating half leaf laver nutritive algal filament in culture fluid, changes one time of nutrition liquid week about.
3. half leaf porphyra breeding method according to claim 2, is characterized in that, described culture fluid is for adding NaNO
3and K
2hPO
3sterilization seawater.
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| CN104094847A (en) * | 2014-07-04 | 2014-10-15 | 常熟理工学院 | Agamogony breeding method of porphyra yezoensis |
| CN104782472A (en) * | 2015-05-14 | 2015-07-22 | 海安县兰波实业有限公司 | Large-scale seedling breeding method for porphyra yezoensis |
| CN104782471A (en) * | 2015-05-14 | 2015-07-22 | 海安县兰波实业有限公司 | Large-scale seedling breeding method for porphyra yezoensis |
| CN105145329B (en) * | 2015-09-15 | 2017-07-11 | 宁波太空红水产养殖有限公司 | It is a kind of to promote Conchospores of Porphyra Hait Anensis to concentrate ripe method |
| CN105993909B (en) * | 2016-05-13 | 2019-08-27 | 集美大学 | A kind of porphyra haitanensis pure lines seedling fostering method |
| CN106489712A (en) * | 2016-10-09 | 2017-03-15 | 盐城海瑞食品有限公司 | The seedling raising process of porphyra haitanensis |
| CN106489713A (en) * | 2016-10-09 | 2017-03-15 | 盐城海瑞食品有限公司 | The method for culturing seedlings of porphyra haitanensis |
| CN106489711A (en) * | 2016-10-09 | 2017-03-15 | 盐城海瑞食品有限公司 | Porphyra haitanensis nursery pond water purification process |
| CN109863993B (en) * | 2019-04-02 | 2021-06-29 | 中国水产科学研究院黄海水产研究所 | In-situ observation method for laver shell protonema |
| CN111066648B (en) * | 2019-12-30 | 2021-10-26 | 中国水产科学研究院黄海水产研究所 | Method for promoting formation of sporocyst branches of shells protonema of half-leaf laver |
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| CN1328950C (en) * | 2004-04-26 | 2007-08-01 | 中国科学院海洋研究所 | Seedling raising method for non-shell protonema cell of laver |
| CN1258969C (en) * | 2004-06-25 | 2006-06-14 | 中国海洋大学 | Porphyra conchocelis pure line making method |
| KR101184318B1 (en) * | 2010-11-10 | 2012-09-19 | 전라남도 | New cultivar of Porphyra yezoensis Ueda Jeonnam super gim 1ho |
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