CN103798123A - Semi-leaf laver seedling cultivating method - Google Patents
Semi-leaf laver seedling cultivating method Download PDFInfo
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- CN103798123A CN103798123A CN201410056088.9A CN201410056088A CN103798123A CN 103798123 A CN103798123 A CN 103798123A CN 201410056088 A CN201410056088 A CN 201410056088A CN 103798123 A CN103798123 A CN 103798123A
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Abstract
The invention discloses a semi-leaf laver seedling cultivating method. The method includes the following steps that semi-leaf laver nutritious trichome is obtained; the semi-leaf laver nutritious trichome is cultivated in nutritious liquid until a protonema of a sporangium branch with the set proportion is formed and the cultivating conditions include that the temperature ranges from 20 DEG C and 30 DEG C, the light intensity ranges from 1000 lx and 2600 lx, the lighting period is 10 hours to 16 hours and the nutritious liquid sea water ratio ranges from 1.026 to 1.031. The protonema of the sporangium branch with the set proportion is placed on an attachment basal body and stimulated by water currents until conchospores of which the density reaches the seedling collecting requirement are formed and seedling cultivating of the semi-leaf laver is finished. Thus, the problem that the seedlings of the semi-leaf laver can not be rapidly and efficiently cultivated with the prior art is solved.
Description
Technical field
The invention belongs to technical field of aquaculture, specifically, relate to a kind of porphyra breeding method, more particularly, relate to a kind of half leaf porphyra breeding method.
Background technology
Laver is all very high tangleweeds of a kind of nutrition and economic worth, and Porphyra kind is more, but China cultivar only has Porphyra haitanensis and porphyra yezoensis at present.Porphyra haitanensis is high-temperature adaptability kind, mainly in Fujian, the coastal cultivation of Zhejiang two provinces, porphyra yezoensis is low temperature adaptability kind, mainly cultivates on a large scale in Jiangsu Province.Porphyra haitanensis and porphyra yezoensis are intertidal zone kind, need periodically dry revealing and Exposure to Sunlight, to remove the assorted algae such as Enteromorpha, diatom and disease, so, the natural conditions in cultivation sea area are had relatively high expectations.Therefore, can only be in intertidal zone, shoal utilizes semi-floating raft type to cultivate, or utilizes bamboo pole to carry out inserted-link type cultivation at offshore compared with deepwater field.And the length of bamboo pole is limited, cause being difficult to expanding compared with deepwater field.In recent years, the cultivation scale of Porphyra haitanensis and porphyra yezoensis had reached threshold value, was difficult to expand again.
And along with the intensification of people to seaweed products nutritive value and green safety cognition, the market of seaweed products is significantly expanded, market is increasing to the demand of laver, occurs the phenomenon that supply falls short of demand.The planting technique that the family active demand of vast laver culture is new and new cultivar, to expand laver area under cultivation, increase laver output, to improve economic well-being of workers and staff.
Half leaf laver is China's Huang, the distinctive kind in Area of Bohai, is mainly grown in the low-lying place of intertidal zone, even still live in water in the time of spring low water.By ecological observation in natural waters to it, find that the anti-adhesive ability of this kind is stronger, the growth busy season, frond color and luster was darker in January to March, and body is larger.As can be seen here, half leaf laver is the kind of suitable cultivation.If seedling growing process maturation, is expected carrying out the cultivation of raft formula compared with abysmal area, be subject to the restriction of shoal cultivation to make up Porphyra haitanensis and porphyra yezoensis.
In the natural life cycle of half leaf laver, be not need experience to do, be exposed to the sun the stage, and, by its stress physiology is studied, find that half leaf laver can not tolerate strong dehydration, freezing processing, so the characteristic of half leaf laver and porphyra yezoensis and Porphyra haitanensis difference are larger.Test shows, while adopting existing porphyra yezoensis, Porphyra haitanensis to grow seedlings, to temperature, illumination, photoperiodic control method, half leaf conchocelis of porphyra cannot form a large amount of sporangial branchlets, and therefore, existing porphyra breeding method is also not suitable for growing seedlings of half leaf laver.At present, not yet occur that double leaf laver carries out the method for high efficiency seedling cultivating, thereby limited the cultivation of half leaf laver.
Summary of the invention
The object of this invention is to provide a kind of half leaf porphyra breeding method, with solve prior art cannot double leaf laver carry out fast, the problem of high efficiency seedling cultivating.
For solving the problems of the technologies described above, the present invention adopts following technical proposals to be achieved:
A kind of half leaf porphyra breeding method, described method comprises the steps:
Obtain half leaf laver nutritive algal filament;
In culture fluid, cultivate half leaf laver nutritive algal filament, until form the filamentous of the sporangial branchlets with preset proportion, condition of culture is: temperature is 20-30 ℃, and light intensity is 1000-2600lx, periodicity of illumination is 10-16h light application time, and culture fluid sea water specific gravity is 1.026-1.031;
The filamentous of the above-mentioned sporangial branchlets with preset proportion is placed on cling matrix, and stimulation by running water, until form the conchospore that meets the demand density of collecting seedling, completes growing seedlings of half leaf laver.
Half leaf porphyra breeding method as above, while cultivating half leaf laver nutritive algal filament, changes one time of nutrition liquid in culture fluid at regular intervals.
Half leaf porphyra breeding method as above, described half leaf laver nutritive algal filament adopts following method to obtain:
Gather half leaf thallus of porphyra, clean, cultivate, filamentous gains freedom;
In culture fluid, cultivate free conchocelis, cultivate a period of time, obtain half leaf laver nutritive algal filament, condition of culture is: temperature is 15-22 ℃, and light intensity is 500-3000lx, and periodicity of illumination is 8-16h light application time, and culture fluid sea water specific gravity is 1.016-1.024.
Half leaf porphyra breeding method as above, the culture fluid of the culture fluid of described cultivation nutrition algal filament and cultivation free conchocelis is all preferably and adds NaNO
3and K
2hPO
3sterilization seawater.
Compared with prior art, advantage of the present invention and good effect are: the present invention is in the time cultivating half leaf laver nutritive algal filament, by controlling sea water specific gravity, in conjunction with suitable temperature, light intensity and periodicity of illumination, can form fast a large amount of cystites and sporangial branchlets, and then realized half leaf laver fast, high efficiency seedling cultivating, be conducive to realize the large-scale farming of half leaf laver.
Read by reference to the accompanying drawings after the specific embodiment of the present invention, it is clearer that the other features and advantages of the invention will become.
Accompanying drawing explanation
Fig. 1 is the flow chart of the present invention half first embodiment of leaf porphyra breeding method;
Fig. 2 to Fig. 5 is the microscopical examination showed intention of half leaf laver nutritive algal filament under different incubation times in Fig. 1 embodiment;
Fig. 6 is the flow chart of the present invention half another embodiment of leaf porphyra breeding method.
Embodiment
Below in conjunction with the drawings and specific embodiments, technical scheme of the present invention is described in further detail.
Refer to Fig. 1, this figure is depicted as the flow chart of the present invention half first embodiment of leaf porphyra breeding method.
As shown in Figure 1, the process that double leaf laver of this embodiment grown seedlings is specific as follows:
Step 11: obtain the half leaf laver nutritive algal filament of having preserved.
Half leaf laver nutritive algal filament is generally kept in culture fluid, needs to control temperature, illumination and photoperiod in preservation process, to maintain nutrition algal filament state.
Step 12: cultivate nutrition algal filament in culture fluid, control condition of culture, until form the filamentous of the sporangial branchlets with preset proportion.
Half leaf laver nutritive algal filament is transferred in the culture fluid that sea water specific gravity is 1.026-1.031, and control cultivation temperature is 20-30 ℃, and light intensity is 1000-2600lx, and periodicity of illumination is 10-16h light application time, cultivates.Wherein, culture fluid is preferably and adds NaNO
3and K
2hPO
3sterilization seawater, and the sea water specific gravity of culture fluid can be by adding appropriate fresh water or NaCl to regulate.Preferred condition of culture is: temperature is 20-22 ℃, and light intensity is 1500-2000lx, and periodicity of illumination is 12h light application time, and sea water specific gravity is 1.026.In cultivation process, within one week, change one time of nutrition liquid, cultivate two months, the thread scale of construction increases to more than 6 times, and the 70% above sporangial branchlets that forms, and meets the requirement of collecting seedling.Under different incubation times, the microscopical examination showed intention of filamentous can be referring to figs. 2 to shown in Fig. 5.
Shown in Fig. 2 to Fig. 5, be respectively the microscopical examination showed intention of nutrition algal filament half leaf conchocelis of porphyra while cultivating 2 weeks, 4 weeks, 7 weeks, two months.As shown in Figure 2, cultivate nutrition algal filament at sea water specific gravity in the culture fluid that is 1.026, cultivate after 2 weeks, half leaf conchocelis of porphyra starts to form and expands (as arrow indication in Fig. 2).Continue to cultivate 4 weeks, as shown in Figure 3, half leaf conchocelis of porphyra forms in a large number and expands (as arrow indication in Fig. 3).Continue to cultivate 7 weeks, as shown in Figure 4, half leaf conchocelis of porphyra forms a large amount of sporangial branchlets, and the new sporangial branchlets forming forms (as arrow indication in Fig. 4) by 1-3 cell.Continue to be cultured to two months, as shown in Figure 5, half leaf conchocelis of porphyra more than 70% forms sporangial branchlets, and sporangial branchlets is by multiple cellularities.Thereby the seedling-cultivating method that this embodiment is described can obtain a high proportion of sporangial branchlets at short notice, and also, the seedling-cultivating method of this embodiment is practicable.
Step 13: the above-mentioned filamentous with sporangial branchlets is placed on cling matrix, and stimulation by running water, forms satisfied collecting seedling and require the conchospore of density.
Prepare large glass box, separate with 1 layer of 300 mesh sieve thin,tough silk near glass box bottom, the filamentous with 70% above sporangial branchlets is placed on the bolting silk of cling matrix, the stimulation by running water that use water temperature is 18-19.5 ℃ three days, get bolting silk microscopy and observe, conchospore adheres to density and on average reaches 30/cm
2, meet the required conchospore density of collecting seedling, complete growing seedlings of half leaf laver.
After this, cling matrix can be moved to deep-sea breeding district together with the conchospore adhering on it cultivates.
This embodiment gathers half leaf laver nutritive algal filament under preservation state, moved in the culture fluid of specific sea water specific gravity, coordinate suitable temperature, light intensity and periodicity of illumination, can form fast a large amount of cystites and sporangial branchlets, and then realized half leaf laver fast, high efficiency seedling cultivating, be conducive to realize the large-scale farming of half leaf laver.
Refer to Fig. 6, the figure shows the flow chart of second embodiment of the present invention's half leaf porphyra breeding method.
As shown in Figure 6, the process that double leaf laver of this embodiment grown seedlings is specific as follows:
Step 21: gather half leaf thallus of porphyra, clean, cultivate, filamentous gains freedom.
From half leaf laver select vegetatively bright-colored, glossiness good, individuality is large, half leaf thallus of porphyra of healthy growth, and thallus is unfolded, and brushes away the attachment such as mud scum, assorted algae on thallus surface with banister bruss, clean 3 times with sterilization seawater.Then, be placed in nutrient solution and cultivate, until the filamentous that gains freedom.
Step 22: cultivate free conchocelis in culture fluid, control condition of culture, obtain half leaf laver nutritive algal filament.
Free conchocelis in step 11 is chosen, be placed in the culture fluid that sea water specific gravity is 1.016-1.024, control cultivation temperature is 15-22 ℃, and light intensity is 500-3000lx, and periodicity of illumination is 8-16h light application time, cultivates.Wherein, culture fluid is preferably and adds NaNO
3and K
2hPO
3sterilization seawater, and the sea water specific gravity of culture fluid can be by adding appropriate fresh water or NaCl to regulate.Preferred condition of culture is: temperature is 20 ℃, and light intensity is 2500lx, and periodicity of illumination is 12h light application time, and sea water specific gravity is 1.018-1.022.In cultivation process, within one week, change one time of nutrition liquid, cultivate one month, obtain enough nutrition algal filaments.
Step 23: cultivate nutrition algal filament in culture fluid, control condition of culture, until form the filamentous of the sporangial branchlets with preset proportion.
The half leaf laver nutritive algal filament that step 22 is obtained is transferred in the culture fluid that sea water specific gravity is 1.026-1.031, and control cultivation temperature is 20-30 ℃, and light intensity is 1000-2600lx, and periodicity of illumination is 10-16h light application time, cultivates.Wherein, culture fluid is preferably and adds NaNO
3and K
2hPO
3sterilization seawater, and the sea water specific gravity of culture fluid can be by adding appropriate fresh water or NaCl to regulate.Preferred condition of culture is: temperature is 25-28 ℃, and light intensity is 2000-2600lx, and periodicity of illumination is 14h light application time, and sea water specific gravity is 1.031.In cultivation process, within one week, change one time of nutrition liquid, cultivate two months, filars lengths increases to more than 5 times, and the 85% above sporangial branchlets that forms, and meets the requirement of collecting seedling.
Step 24: the above-mentioned filamentous with sporangial branchlets is placed on cling matrix, and stimulation by running water, forms satisfied collecting seedling and require the conchospore of density.
Preparing plastic containing box, the use of collecting seedling on tiling at the bottom of case nettle, is placed on the filamentous with 85% above sporangial branchlets on the nettle of cling matrix, the stimulation by running water that use water temperature is 23-25 ℃ three days, get nettle microscopy and observe, conchospore adheres to density and on average reaches 25/cm
2, meet the required conchospore density of collecting seedling, complete growing seedlings of half leaf laver.
After this, cling matrix can be moved to deep-sea breeding district together with the conchospore adhering on it cultivates.
This embodiment directly gathers half leaf thallus of porphyra, first turn out free conchocelis, then under specific sea water specific gravity, turn out again nutrition algal filament, thereafter, again nutrition algal filament is moved in the culture fluid that sea water specific gravity is greater than free conchocelis place culture fluid, coordinate suitable temperature, light intensity and periodicity of illumination, can form fast a large amount of cystites and sporangial branchlets, and then realized half leaf laver fast, high efficiency seedling cultivating, be conducive to realize the large-scale farming of half leaf laver.
Above embodiment is only in order to technical scheme of the present invention to be described, but not is limited; Although the present invention is had been described in detail with reference to previous embodiment, for the person of ordinary skill of the art, the technical scheme that still can record previous embodiment is modified, or part technical characterictic is wherein equal to replacement; And these modifications or replacement do not make the essence of appropriate technical solution depart from the spirit and scope of the present invention's technical scheme required for protection.
Claims (5)
1. a half leaf porphyra breeding method, is characterized in that, described method comprises the steps:
Obtain half leaf laver nutritive algal filament;
In culture fluid, cultivate half leaf laver nutritive algal filament, until form the filamentous of the sporangial branchlets with preset proportion, condition of culture is: temperature is 20-30 ℃, and light intensity is 1000-2600lx, periodicity of illumination is 10-16h light application time, and culture fluid sea water specific gravity is 1.026-1.031;
The filamentous of the above-mentioned sporangial branchlets with preset proportion is placed on cling matrix, and stimulation by running water, until form the conchospore that meets the demand density of collecting seedling, completes growing seedlings of half leaf laver.
2. half leaf porphyra breeding method according to claim 1, is characterized in that, while cultivating half leaf laver nutritive algal filament, changes at regular intervals one time of nutrition liquid in culture fluid.
3. half leaf porphyra breeding method according to claim 2, is characterized in that, described culture fluid is for adding NaNO
3and K
2hPO
3sterilization seawater.
4. according to half leaf porphyra breeding method described in any one in claims 1 to 3, it is characterized in that, described half leaf laver nutritive algal filament adopts following method to obtain:
Gather half leaf thallus of porphyra, clean, cultivate, filamentous gains freedom;
In culture fluid, cultivate free conchocelis, cultivate a period of time, obtain half leaf laver nutritive algal filament, condition of culture is: temperature is 15-22 ℃, and light intensity is 500-3000lx, and periodicity of illumination is 8-16h light application time, and culture fluid sea water specific gravity is 1.016-1.024.
5. half leaf porphyra breeding method according to claim 4, is characterized in that, described culture fluid is for adding NaNO
3and K
2hPO
3sterilization seawater.
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| CN104094847A (en) * | 2014-07-04 | 2014-10-15 | 常熟理工学院 | Agamogony breeding method of porphyra yezoensis |
| CN104782472A (en) * | 2015-05-14 | 2015-07-22 | 海安县兰波实业有限公司 | Large-scale seedling breeding method for porphyra yezoensis |
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| CN105145329A (en) * | 2015-09-15 | 2015-12-16 | 宁波太空红水产养殖有限公司 | Method for promoting concentrated maturity of conchospores of porphyra haitanensis |
| CN105993909A (en) * | 2016-05-13 | 2016-10-12 | 集美大学 | Pyropia haitanensis pure line seedling cultivation method |
| CN106489712A (en) * | 2016-10-09 | 2017-03-15 | 盐城海瑞食品有限公司 | The seedling raising process of porphyra haitanensis |
| CN106489711A (en) * | 2016-10-09 | 2017-03-15 | 盐城海瑞食品有限公司 | Porphyra haitanensis nursery pond water purification process |
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| CN109863993A (en) * | 2019-04-02 | 2019-06-11 | 中国水产科学研究院黄海水产研究所 | The home position observation method of one main laver shell conchocelis |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104094847A (en) * | 2014-07-04 | 2014-10-15 | 常熟理工学院 | Agamogony breeding method of porphyra yezoensis |
| CN104782472A (en) * | 2015-05-14 | 2015-07-22 | 海安县兰波实业有限公司 | Large-scale seedling breeding method for porphyra yezoensis |
| CN104782471A (en) * | 2015-05-14 | 2015-07-22 | 海安县兰波实业有限公司 | Large-scale seedling breeding method for porphyra yezoensis |
| CN105145329A (en) * | 2015-09-15 | 2015-12-16 | 宁波太空红水产养殖有限公司 | Method for promoting concentrated maturity of conchospores of porphyra haitanensis |
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| CN106489711A (en) * | 2016-10-09 | 2017-03-15 | 盐城海瑞食品有限公司 | Porphyra haitanensis nursery pond water purification process |
| CN106489713A (en) * | 2016-10-09 | 2017-03-15 | 盐城海瑞食品有限公司 | The method for culturing seedlings of porphyra haitanensis |
| CN109863993A (en) * | 2019-04-02 | 2019-06-11 | 中国水产科学研究院黄海水产研究所 | The home position observation method of one main laver shell conchocelis |
| CN109863993B (en) * | 2019-04-02 | 2021-06-29 | 中国水产科学研究院黄海水产研究所 | In-situ observation method for laver shell protonema |
| CN111066648A (en) * | 2019-12-30 | 2020-04-28 | 中国水产科学研究院黄海水产研究所 | Method for promoting formation of sporocyst branches of shells protonema of half-leaf laver |
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