CN104012432A - Method suitable for artificial breeding of CrassOstrea hongkongensis in sea area in north - Google Patents

Method suitable for artificial breeding of CrassOstrea hongkongensis in sea area in north Download PDF

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CN104012432A
CN104012432A CN201410184810.7A CN201410184810A CN104012432A CN 104012432 A CN104012432 A CN 104012432A CN 201410184810 A CN201410184810 A CN 201410184810A CN 104012432 A CN104012432 A CN 104012432A
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sea area
shellfish
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于瑞海
王昭萍
马培振
佘忠明
陈洪发
刘剑
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Ocean University of China
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Ocean University of China
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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Abstract

The invention discloses a method suitable for artificial breeding of CrassOstrea hongkongensis in a sea area in the north. The method suitable for artificial breeding of CrassOstrea hongkongensis in the sea area in the north is characterized by comprising the steps that firstly, CrassOstrea hongkongensis adult shellfish with a shell higher than 10 cm are selected, the CrassOstrea hongkongensis adult shellfish are placed in breeding ponds in high-temperature summer (July and August) with the density of 40-50 pieces/m<3> under the conditions that the water temperature ranges from 26 DEG C to 30 DEG C and the salinity ranges from 25 to 32, artificial oviposition and spermiation are conducted after the CrassOstrea hongkongensis adult shellfish are bred for 10 days to 15 days, spawn and sperms are placed in a breeding pond containing filtered sea water to be discharged naturally after being dried in the shade for five hours to eight hours, or the spawn and the sperms are obtained after the male shellfish and female shellfish are distinguished manually, and then artificial insemination is conducted; germ cells are hatched for 18 hours to 20 hours under the condition that the water temperature ranges from 26 DEG C to 32 DEG C so that D type larvas can be obtained, the D type larvas are placed in ponds to be bred with the density of 6-8 pieces/m<3>, when 50%-80% of the D type larvas become eyespot larvas after the D type larvas are hatched for 15 days to 18 days, oyster shell and cement block substrata are placed into the ponds, and juvenile mollusk is obtained after the eyespot larvas adheres to the oyster shell and cement block substrata for four days to six days; finally, the juvenile mollusk adhering to the oyster shell and cement block substrata is moved into outdoor ponds to be bred, the juvenile mollusk is transported to a sea area in the south to be bred in an overland mode at lower temperature after the juvenile mollusk is 5 mm-10 mm long. By the adoption of the method suitable for artificial breeding of CrassOstrea hongkongensis in the sea area in the north, the problems that the rate of fertilization for artificial breeding of the CrassOstrea hongkongensis in the south is low and the survival rate of the larvas is low are solved.

Description

一种适宜北方海区香港巨牡蛎人工育苗的方法A method suitable for artificial breeding of Hong Kong giant oyster in the northern sea area

技术领域technical field

本发明涉及一种香港巨牡蛎苗种生产的方法,特别是涉及一种适宜北方海区香港巨牡蛎人工育苗的方法。The invention relates to a method for producing seedlings of Hong Kong giant oyster, in particular to a method suitable for artificial breeding of Hong Kong giant oyster in the northern sea area.

背景技术Background technique

香港巨牡蛎(CrassOstrea hongkongensis)为高温低盐种,栖息于近河口或附近有淡水注入的地方,已有700多年的养殖历史,是我国南方海区特有经济贝类,其壳质坚厚,软体部为白色、外套膜多为黑色,具有色泽美观、肉质鲜美的特点,市场价格比长牡蛎等其他牡蛎高1-2倍,其市场前景广阔。The Hong Kong giant oyster (CrassOstrea hongkongensis) is a high-temperature and low-salt species that inhabits near the estuary or near the place where fresh water is injected. It has a breeding history of more than 700 years. It is a unique economic shellfish in the southern sea area of my country. It is white, and the mantle is mostly black. It has the characteristics of beautiful color and delicious meat. The market price is 1-2 times higher than other oysters such as long oysters, and its market prospect is broad.

目前我国从香港巨牡蛎苗种主要以半人工采苗为主,但野生苗种的采苗受季节限制,产量不稳定,限制了香港巨牡蛎的养殖业的发展.随着养殖规模的扩大,对香港巨牡蛎苗种的需求越来越多,由于香港巨牡蛎人工育苗受育苗技术、设施和条件的影响,育苗成功率较低,眼点幼虫的附着变态是其技术瓶颈,因而造成香港巨牡蛎育苗出苗量极低,达不到生产性育苗的水平,满足不了养殖单位对苗种的需求,针对香港巨牡蛎育苗技术难题,开发新的育苗方式是解决难题的关键,同时还可以克服香港巨牡蛎自然苗生长速度慢,养殖周期长的问题。At present, China's giant oyster seedlings from Hong Kong are mainly semi-artificially collected seedlings, but the collection of wild seedlings is limited by seasons, and the output is unstable, which limits the development of the Hong Kong giant oyster aquaculture industry. With the expansion of the breeding scale, There is more and more demand for Hong Kong giant oyster seedlings. Due to the influence of Hong Kong giant oyster seedlings on seedling technology, facilities and conditions, the success rate of seedlings is low, and the attachment and metamorphosis of eye-spot larvae is the technical bottleneck, which has caused the Hong Kong giant oyster. The emergence of oyster seedlings is extremely low, which cannot reach the level of productive seedlings, and cannot meet the needs of breeding units for seedlings. Aiming at the technical problems of giant oyster seedlings in Hong Kong, developing new seedling methods is the key to solving the problems. Giant oyster natural seedlings grow slowly and have a long breeding cycle.

发明内容Contents of the invention

本发明的目的是提供一种适宜北方海区香港巨牡蛎人工育苗的方法,能克服现有技术的上述缺点。The purpose of the present invention is to provide a method suitable for artificial seedling cultivation of Hong Kong giant oyster in the northern sea area, which can overcome the above-mentioned shortcomings of the prior art.

一种适宜北方海区香港巨牡蛎人工育苗的方法,其特征是首先在南方养殖海区选择壳高10cm以上香港巨牡蛎成贝,低温运到北方海区的室外海水池塘里蓄养培育;然后,选择正规的贝类育苗场,在夏季(7-8月)高温季节,挑选出香港巨牡蛎种贝,按40-50个/m3放在育苗池里,在水温26-30℃和盐度25-32的条件下,亲贝按常规贝类室内人工促熟方法培育10-15天后,进行人工产卵、排精,产卵排精采用阴干5-8小时后,放入盛过滤海水育苗池中自然排放,也可人工辨别雌雄亲贝后获取精、卵,进行人工授精;在水温26-32℃条件下,受精卵经过18-20小时孵化达到D型幼虫,经选优按6-8个/m3密度布池培育,幼虫培育按常规贝类幼虫培育技术进行,经15-18天培育50-80%出现眼点幼虫时,开始投放牡蛎壳和水泥块附着基,香港巨牡蛎的眼点幼虫经4-6天附着变态成稚贝;最后将附着稚贝的附着基一起移到室外池塘内培育内,稚贝利用池塘内的单细胞藻类生长发育,长到5-10mm以后低温干运至南方海区进行养殖。A method suitable for artificial breeding of Hong Kong giant oysters in the northern sea area is characterized in that firstly the Hong Kong giant oyster with a shell height of more than 10 cm is selected in the southern sea area for cultivation, and the shells are transported to the outdoor seawater ponds in the northern sea area at low temperature for storage and cultivation; In the shellfish nursery, in the summer (July-August) high temperature season, Hong Kong giant oyster seed shells are selected and placed in the nursery pond at a rate of 40-50/m 3 . Under certain conditions, broilers are cultured for 10-15 days according to the conventional method of indoor artificial ripening of shellfish, and then artificially spawn and ejaculate. Discharge, or artificially distinguish male and female broodstock to obtain sperm and eggs for artificial insemination; under the condition of water temperature 26-32°C, the fertilized eggs will hatch into D-type larvae after 18-20 hours, and the selection is optimal at 6-8 pcs/ Cultivate ponds with a density of m3 , and cultivate larvae according to conventional shellfish larvae cultivation techniques. After 15-18 days of cultivation, when 50-80% of the eye-spot larvae appear, start to put oyster shells and cement block attachment bases, the eye-spots of Hong Kong giant oyster After 4-6 days, the larvae attach and metamorphose into spat; finally, the attachment base attached to the spat is moved to an outdoor pond for cultivation, and the spat use the single-celled algae in the pond to grow and develop, and after they grow to 5-10mm, they are transported dry at low temperature to the southern sea area for breeding.

本发明解决了南方香港巨牡蛎室内人工育苗受精率低、幼虫成活率低,附着变态率低的问题。同时本发明可以利用北方高温期贝类育苗车间空闲期,实现香港巨牡蛎北方人工育苗,南方养殖,克服自然采苗生长速度慢的问题,缩短香港巨牡蛎养殖时间。The invention solves the problems of low fertilization rate, low survival rate of larvae and low attachment metamorphosis rate of giant oyster indoor artificial breeding in southern Hong Kong. At the same time, the present invention can utilize the idle period of the shellfish nursery workshop in the high temperature period in the north to realize the artificial seedling cultivation of Hong Kong giant oyster in the north and culture in the south, overcome the problem of slow growth of natural seedlings, and shorten the cultivation time of Hong Kong giant oyster.

具体实施方式Detailed ways

一种适宜北方海区香港巨牡蛎人工育苗的方法,其特征是首先在南方养殖海区选择壳高10cm以上香港巨牡蛎成贝,低温运到北方海区的室外海水池塘里蓄养培育;然后,选择正规的贝类育苗场,在夏季(7-8月)高温季节,挑选出香港巨牡蛎种贝,按40-50个/m3放在育苗池里,在水温26-30℃和盐度25-32的条件下,亲贝按常规贝类室内人工促熟方法培育10-15天后,进行人工产卵排精,产卵排精采用阴干5-8小时后,放入盛过滤海水育苗池中自然排放,也可人工解剖亲贝辨别雌雄后获取精、卵,进行人工授精;在水温26-32℃条件下,受精卵经过18-20小时孵化达到D型幼虫,经选优按6-8个/m3密度布池培育,幼虫培育按常规贝类幼虫培育技术进行,经15-18天培育50-80%出现眼点幼虫时,开始投放牡蛎壳和水泥块附着基,香港巨牡蛎的眼点幼虫经4-6天附着变态成稚贝;最后将附着稚贝的附着基一起移到室外池塘内培育内,稚贝利用池塘内的单细胞藻类生长发育,长到5-10mm以后低温干运至南方海区进行养殖。A method suitable for artificial breeding of Hong Kong giant oyster in the northern sea area is characterized in that firstly, the Hong Kong giant oyster with a shell height of more than 10 cm is selected in the southern sea area as a shellfish, and transported to the outdoor seawater pond in the northern sea area at low temperature for storage and cultivation; In the shellfish nursery, in the high temperature season of summer (July-August), Hong Kong giant oyster seed shells are selected and placed in the nursery pond at a rate of 40-50/m 3 . Under certain conditions, broilers are cultured for 10-15 days according to the conventional method of indoor artificial ripening of shellfish, and then artificially spawn and ejaculate, and after 5-8 hours of drying in the shade for oviposition and ejaculation, they are placed in a filtered seawater nursery pond for natural discharge , can also be manually dissected to distinguish between male and female, obtain sperm and eggs, and carry out artificial insemination; under the condition of water temperature 26-32 ℃, the fertilized eggs will hatch into D-type larvae after 18-20 hours, and the optimal selection is 6-8/ Cultivate ponds with a density of m3 , and cultivate larvae according to conventional shellfish larvae cultivation techniques. After 15-18 days of cultivation, when 50-80% of the eye-spot larvae appear, start to put oyster shells and cement block attachment bases, the eye-spots of Hong Kong giant oyster After 4-6 days, the larvae attach and metamorphose into spat; finally, move the attachment base attached to the spat to an outdoor pond for cultivation, and the spat use the single-celled algae in the pond to grow and develop, and dry transport at low temperature after they grow to 5-10mm to the southern sea area for breeding.

本发明解决了南方香港巨牡蛎室内人工育苗受精率低、幼虫成活率低,附着变态率低的问题。同时本发明可以利用北方高温期贝类育苗车间空闲期,实现香港巨牡蛎北方人工育苗,南方养殖,克服自然采苗生长速度慢的问题,缩短香港巨牡蛎养殖时间,提高了夏季高温期北方大量育苗车间的利用率。The invention solves the problems of low fertilization rate, low survival rate of larvae and low attachment metamorphosis rate of giant oyster indoor artificial breeding in southern Hong Kong. Simultaneously, the present invention can make use of the idle period of the shellfish nursery workshop in the high temperature period in the north to realize artificial seedling cultivation in the north of Hong Kong giant oyster and culture in the south, overcome the problem of slow growth rate of natural seedlings, shorten the breeding time of Hong Kong giant oyster, and increase the number of large oysters in the north during the high temperature period in summer. Utilization rate of nursery workshop.

Claims (1)

1.一种适宜北方海区香港巨牡蛎人工育苗的方法,其特征是首先在南方养殖海区选择壳高10cm以上香港巨牡蛎成贝,低温运到北方海区的室外海水池塘里蓄养培育;然后,选择正规的贝类育苗场,在夏季(7-8月)高温季节,挑选出香港巨牡蛎种贝,按40-50个/m3放在育苗池里,在水温26-30℃和盐度25-32的条件下,亲贝按常规贝类室内人工促熟方法培育10-15天后,进行人工产卵、排精,产卵排精采用阴干5-8小时后,放入育苗池中自然排放,也可人工辨别雌雄亲贝后获取精、卵,进行人工授精;在水温26-32℃条件下,受精卵经过18-20小时孵化达到D型幼虫,经选优按6-8个/m3密度布池培育,幼虫培育按常规贝类幼虫培育技术进行,经15-18天培育50-80%出现眼点幼虫时,开始投放牡蛎壳和水泥块附着基,香港巨牡蛎的眼点幼虫经4-6天附着变态成稚贝;最后将附着稚贝的附着基一起移到室外池塘内培育内,稚贝利用池塘内的单细胞藻类生长发育,长到5-10mm以后低温干运至南方海区进行养殖。1. A method suitable for artificial breeding of Hong Kong giant oyster in the northern sea area is characterized in that at first the Hong Kong giant oyster with a shell height of more than 10cm is selected in the southern sea area to become a shellfish, and the low temperature is transported to the outdoor seawater pond in the northern sea area to raise and cultivate; then, select Formal shellfish nursery farms, in the summer (July-August) high temperature season, select Hong Kong giant oyster seed shells, put them in nursery ponds at a rate of 40-50/m 3 , at a water temperature of 26-30°C and a salinity of 25°C. Under the condition of -32, broilers are cultured for 10-15 days according to the conventional method of artificial ripening of shellfish indoors, then artificially lay eggs and ejaculate, and dry them in the shade for 5-8 hours after oviposition and ejaculation, and then put them into the nursery pond for natural discharge , can also manually distinguish male and female broodstock, obtain sperm and eggs, and carry out artificial insemination; under the condition of water temperature 26-32 ℃, the fertilized eggs will hatch to D-type larvae after 18-20 hours, and the optimal selection is 6-8 per m 3- density cloth pond cultivation, larvae cultivation is carried out according to the conventional shellfish larvae cultivation technology, after 15-18 days of cultivation, when 50-80% of the eye-spot larvae appear, start to put oyster shells and cement block attachment base, the eye-spot larvae of Hong Kong giant oyster After 4-6 days of attachment and metamorphosis, the spat will become attached to the spat; finally, the attachment base attached to the spat will be moved to the outdoor pond for cultivation. The spat will grow and develop with the single-celled algae in the pond, and will be transported dry at low temperature after growing to 5-10mm. Breeding in the southern sea area.
CN201410184810.7A 2014-04-25 2014-04-25 Method suitable for artificial breeding of CrassOstrea hongkongensis in sea area in north Pending CN104012432A (en)

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CN106719185A (en) * 2017-01-05 2017-05-31 中国海洋大学 A kind of method that rock oyster ecological breeding is carried out using the megathermal period
CN106719185B (en) * 2017-01-05 2020-05-19 中国海洋大学 Method for ecologically breeding crassostrea gigas seedlings by utilizing high-temperature period
CN106942105A (en) * 2017-05-11 2017-07-14 中国海洋大学 A kind of method for carrying out Hong Kong oyster artificial breeding using the northern megathermal period
CN106942105B (en) * 2017-05-11 2020-03-17 中国海洋大学 Method for artificially breeding seedlings of crassostrea hongkongensis by utilizing northern high-temperature period
CN110984405A (en) * 2019-12-02 2020-04-10 哈尔滨工程大学 An ecological anti-corrosion method for marine reinforced concrete engineering in service
CN110984405B (en) * 2019-12-02 2021-12-24 哈尔滨工程大学 Ecological anti-corrosion method for serving marine reinforced concrete engineering
CN111183936A (en) * 2020-02-11 2020-05-22 中国科学院南海海洋研究所 Method for preparing novel strain of deep concave-shell type hongkong oyster by using virtual index polymerization method
CN111316943A (en) * 2020-03-07 2020-06-23 广西壮族自治区海洋研究所 Pond seedling raising method for ostrea rivularis
CN115943914A (en) * 2022-05-13 2023-04-11 中国海洋大学 A kind of cultivation method of rock oyster spat
CN115005136A (en) * 2022-05-25 2022-09-06 中国科学院烟台海岸带研究所 A high-efficiency breeding method for high temperature resistant strains of the northern coastal clam
CN115005136B (en) * 2022-05-25 2023-03-21 中国科学院烟台海岸带研究所 Efficient culture method for high-temperature-resistant variety of northern coastal blood clam
CN119605703A (en) * 2025-02-11 2025-03-14 海南晨海水产有限公司 A method for artificial breeding of grouper suitable for high-latitude areas

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Application publication date: 20140903