CN103975879B - The breeding method of the golden yellow fast lady's fingers tooth oyster new lines of a kind of shell - Google Patents

The breeding method of the golden yellow fast lady's fingers tooth oyster new lines of a kind of shell Download PDF

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CN103975879B
CN103975879B CN201410197671.1A CN201410197671A CN103975879B CN 103975879 B CN103975879 B CN 103975879B CN 201410197671 A CN201410197671 A CN 201410197671A CN 103975879 B CN103975879 B CN 103975879B
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oyster
shell
breeding
golden yellow
seedling
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CN201410197671.1A
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CN103975879A (en
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曾志南
宁岳
祁剑飞
巫旗生
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福建省水产研究所
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention discloses the breeding method of the golden yellow fast lady's fingers tooth oyster new lines of a kind of shell, it comprises the steps: to formulate breeding goal, set up the step such as breeding line, new lines purifying.To be specially with the golden yellow shell of Portuguese oyster and full weight as breeding goal, to set up breeding line by unexpected mass incident and family selective breeding method, filter out the golden yellow fast lady's fingers tooth oyster new lines of shell by the directed purifying in consecutive numbers generation.To be had for the Portuguese oyster new lines of seed selection by consecutive numbers that growth is fast, shell is golden yellow, commodity value advantages of higher, it is suitable for as cultchless oyster cultivation and eats oyster production raw, and this has important function for raising Portuguese oyster cultured output, commodity value and economic benefit.

Description

The breeding method of the golden yellow fast lady's fingers tooth oyster new lines of a kind of shell

Technical field

The present invention relates to aquatic products genetic breeding field, relate to the breeding method of the golden yellow fast lady's fingers tooth oyster new lines of a kind of shell particularly.

Background technology

Oyster (Ostreidae) belongs to Mollusca (Mollusca), Bivalvia (Bivalvia), pearl shell order (Pterioidae), Ostreidae (Ostreidae), is important marine organisms.Its meat flavour is delicious, and nutritive value is higher, have the laudatory title of " marine milk ", is the important mariculture objects in countries in the world, is also the maximum economic shellfish of China and even world's cultured output.It is the first that Chinese Oysters output occupies the whole world, and Fujian is the oyster culture district of Largest In China, mainly cultivates Portuguese oyster (Crassostrea angulata), and within 2012, Portuguese oyster cultured output has accounted for more than 37% of national oyster output.But along with increasing year by year of Portuguese oyster cultured area and improving constantly of output, also there is kind of a matter degradation phenomena, the original characteristic sum quality of Portuguese oyster changes, outstanding behaviours in recent years reduces in nursery success rate, often there is death in enormous quantities in oyster culture, and present cultivation individual miniaturized, grow the economic characters continuous downturn phenomenons such as slow, have impact on cultured output and the quality of oyster.In addition, traditional breeding way is still continued to use in the oyster culture of current China, and product is mainly sold to open shell fresh meat, and the output value is low, and culture benefit is not high, differs greatly compared with external oyster price.Therefore cultivate high yield, high-quality oyster new lines, realize the industrialization of oyster breeding, significant to promotion China oyster culture industry sustainable and healthy development.

Shell look, as a heritable qualitative character, has obtained better achievement in some shellfish genetic breeding researchs such as scallop.The present invention adopts selection and use method, and with oyster shell, golden yellow and full weight is for breeding goal, carry out the seed selection of golden yellow shell look fast lady's fingers tooth oyster new lines, the new lines not only fast growth of cultivation, and shell is golden yellow, color is gorgeous.The very suitable monomer oyster culture and eat oyster raw and produce, to improve the cultured output of Portuguese oyster, commodity value and economic benefit of this strain.

Summary of the invention

The object of the present invention is to provide the breeding method of the golden yellow fast lady's fingers tooth oyster new lines of a kind of shell.

Technical scheme provided by the invention is as follows:

A breeding method for the golden yellow fast lady's fingers tooth oyster new lines of shell, it comprises the steps:

1) close shellfish is selected: from a large amount of cultured population of Portuguese oyster, select 2 ages more than, and shell is in the individuality golden yellow, color is gorgeous, shell mould is regular; Measure the full weight of all picking individual, before then intercepting full weight, the male and female of 10% are individual as breeding close shellfish;

2) smart ovum obtains: dissect close shellfish, distinguish male and female, carried out respectively by close shellfish good for gonad development washing ovum, washing essence, and with 300 mesh sieve thin,tough silk impurity screenings, ovum is collected in the white bucket of 160L plastics of mark respectively, and seminal fluid is collected in the 500ml beaker of mark respectively;

3) artificial insemination and hatching:

A. mass mating: get male and female parent shellfish each 20-25, its ovum and sperm mixing mating;

B. stud mating: get male and female parent shellfish, carry out male and female individuality 1 to 1 mating, set up family;

The white bucket of 160L plastics each group of fertilized egg being placed in respectively mark is hatched, and seminal concentration is advisable with 3-4 sperm around each ovum, adopts the precipitation method to wash ovum 2-3 time during polyspermism; Triploidy rate 40-50/ml, micro-inflation, hatching water temperature 25-30 DEG C;

4) larvae cultivation: development of fertilized ova is cultivated often organizing larvae collection in three white buckets of 160L plastics marked respectively to adopting siphon method during D type larva, often organizes 3 parallel group; D type larvae cultivation density domination, at 8-12/ml, along with larval growth reduces density gradually, is down to 1-2/ml to Creeping larva density; Change water every day 2 times, change water 30%-50% at every turn, after larva attachment, increase to 60%-100%; D type larva starts chrysophyceae of throwing something and feeding, and daily ration of feeding increases to 2-3 ten thousand cells/ml in mid-term gradually from the 1-2 ten thousand cells/ml at initial stage, later stage 3-4 ten thousand cells/ml, and larvae development adds flat algae to the shell top later stage, and daily ration of feeding is 1-2 ten thousand cells/ml; Can suitably throw something and feed during larvae cultivation Chaetoceros or chlorella, water temperature 25-30 DEG C during larvae cultivation, salinity 23-28, pH value 8.0-8.5, about need 14-20 days from D type larvae cultivation to Creeping larva;

5) collect seedling: start to throw in collector when larval growth occurs eyespot to shell long 320-330um, 70-80%; The gray tinted plastic sheet that collector adopts non-toxic material to make, plastic sheet specification is long 20-30cm, wide 1-3cm, thick 0.1-0.3cm; With 60-80 silk polyethylene rope by the plastic sheet string cleaned together, tie up and on cross bar, hang on 60-80cm place, nursery pond underwater collect seedling, every cubic metre of water body throws in plastic sheet 30-40 string; Every sheet plastic sheet spatfall 20-30; Oyster seedling grows to long about the 1-2mm of shell, marks each breeding line and move to sea area to carry out seed intermediate rearing;

In larvae cultivation process, keep each group of breeding line larval density identical, daily ration, feeding quantity is consistent with rate of water exchange, and breeding condition is identical; Container for plant growth, the utensil of each group of breeding line individually use, and avoid mixing;

6) sea area oyster seed intermediate rearing: the collector enclosing oyster seedling of mark is moved to same a slice sea area and cultivates; If meet the natural waters Portuguese oyster larva setting stage, first can move to pond rearing, move to sea area after the phase to be attached again and cultivate; Water temperature 13-26 DEG C, under the condition of salinity 26-30, cultivate through 90-120 days sea areas, oyster seedling can grow to the long 4-5cm of shell;

7) in oyster, seedling takes off base and monomer cultivation: when oyster seedling grows to 4-5cm, bending oyster adherance plastic sheet, and oyster seedling just comes off smoothly, forms cultchless oyster seedling; The cultivation basket that the cultchless oyster seedling peeled off by each breeding line loads mark is hung on the cultivation of same a slice sea area; Periodic cleaning aufwuch during cultivation, and measure growing state;

8) new lines purifying: select to possess individual large, individuality that the golden yellow proterties of shell, shell mould are regular as second generation parent from the golden yellow fast lady's fingers tooth oyster breeding line first generation of shell, the each step of second generation seed selection with first generation selection, until select the golden yellow fast lady's fingers tooth oyster strain of the stable shell of proterties.

Wherein, step 5) in, plastic sheet of collecting seedling often goes here and there 16-20 sheet, pitch of fins 13-17cm.

Wherein, step 5) in, at water temperature 25-30 DEG C, cultivated through 5-6 days after larva set, oyster seedling can grow to the long 1-2mm of shell.

Wherein, step 6) in, under water temperature 13-26 DEG C of condition, cultivate through 90-120 days sea areas, oyster seedling can grow to the long 4-5cm of shell.

Seen from the above description, the invention discloses the breeding method of the golden yellow fast lady's fingers tooth oyster new lines of a kind of shell, it comprises the steps: to formulate breeding goal, set up the step such as breeding line, new lines purifying.To be specially with the golden yellow shell of Portuguese oyster and full weight as breeding goal, to set up breeding line by unexpected mass incident and family selective breeding method, filter out the golden yellow fast lady's fingers tooth oyster new lines of shell by the directed purifying in consecutive numbers generation.To be had for the Portuguese oyster new lines of seed selection by consecutive numbers that growth is fast, shell is golden yellow, commodity value advantages of higher, it is suitable for as cultchless oyster cultivation and eats oyster production raw, and this has important function for raising Portuguese oyster cultured output, commodity value and economic benefit.

Embodiment

In Portuguese oyster cultured population, have minimum ratio oyster shell in golden yellow, color is gorgeous, has higher commodity value.This method with the golden yellow shell of oyster and full weight for breeding goal.

1st generation seed selection

(1) close shellfish is selected

In late August, 2010 selects 2 ages more than from a large amount of cultured population of Portuguese oyster, and shell is in the individuality golden yellow, color is gorgeous, shell mould is regular.Measure the full weight of all picking individual, before then intercepting full weight, close shellfishes are bred in individual 150 conducts of the male and female of 10%.

(2) smart ovum obtains

Dissect close shellfish, distinguish male and female, close shellfish good for gonad development is carried out respectively wash ovum, wash essence, and with 300 mesh sieve thin,tough silk impurity screenings, ovum is collected in the white bucket of 160L plastics of mark respectively, and seminal fluid is collected in the 500ml beaker of mark respectively.

(3) artificial insemination and hatching

1. mass mating: get male and female parent each 25 of shellfish, its ovum and sperm mixing mating;

2. stud mating: get male and female parent shellfish, carry out male and female individuality 1 to 1 mating, set up family;

The white bucket of 160L plastics each group of fertilized egg being placed in respectively mark is hatched, incubating oosperm density 40-50/ml, micro-inflation, hatching water temperature 28-30 DEG C.

(4) larvae cultivation

Development of fertilized ova is cultivated often organizing larvae collection (often organizing 3 parallel group) respectively to adopting siphon method during D type larva in three white buckets of 160L plastics marked.D type larvae cultivation density 10/about ml, along with larval growth reduces density gradually, is down to 1-2/ml to Creeping larva density.Change water every day 2 times, change water 30%-50% at every turn, after larva attachment, increase to 60%-100%.D type larva starts chrysophyceae of throwing something and feeding, and daily ration of feeding increases to 2-3 ten thousand cells/ml in mid-term gradually from the 1-2 ten thousand cells/ml at initial stage, later stage 3-4 ten thousand cells/ml, and larvae development adds flat algae to the shell top later stage, and daily ration of feeding is 1-2 ten thousand cells/ml.Can suitably to throw something and feed during larvae cultivation Chaetoceros or chlorella.Water temperature 28-30 DEG C during larvae cultivation, salinity 27, pH value 8.2.From D type larvae cultivation to Creeping larva 17 days.

(5) collect seedling

Start to throw in collector (adherance) when larval growth occurs eyespot to shell long 320-330um, 70-80%.The gray tinted plastic sheet that collector adopts nonpoisonous polypropylene to make, plastic sheet specification is long 24cm, wide 2cm, thick 0.2cm.With 60-80 silk polyethylene rope by clean plastic sheet string together, often go here and there 18, pitch of fins 15cm.Be advisable for every sheet plastic sheet spatfall 20-30.Under water temperature 28-30 DEG C of condition, cultivated through 6 days after larva set, oyster seedling grows to the long 1-2mm of shell, marks each breeding line and move to sea area to carry out seed intermediate rearing.

In larvae cultivation process, keep each group of breeding line larval density identical, daily ration, feeding quantity is consistent with rate of water exchange, and breeding condition is identical; Container for plant growth, the utensil of each group of breeding line individually use, and avoid mixing.

(6) sea area oyster seed intermediate rearing

The collector enclosing oyster seedling of mark is moved to Shenhu Bay of Fujian Province sea area cultivate.Water temperature 13-26 DEG C, under the condition of salinity 26-30, cultivate through 115 days sea areas, oyster seedling can grow to the long 4-5cm of shell.

(7) in oyster, seedling takes off base and monomer cultivation

When oyster seedling grows to 4-5cm, bending oyster adherance plastic sheet, oyster seedling just comes off smoothly, forms cultchless oyster seedling.The cultivation basket that the cultchless oyster seedling peeled off by each breeding line loads mark is hung on the cultivation of Shenhu Bay of Fujian Province sea area.Periodic cleaning aufwuch during cultivation, and measure growing state.

Select through close shellfish, smart ovum obtain, artificial insemination and hatching, larvae cultivation, collect seedling, sea area oyster seed intermediate rearing, seedling takes off the step such as base and monomer cultivation in oyster, obtains the fast lady's fingers tooth oyster breeding line of 1st generation shell golden yellow.In late August, 2011,1st generation was developed to sexual maturity, namely carried out 2nd generation seed selection.

2nd generation seed selection

(1) close shellfish is selected

In late August, 2011, from the golden yellow fast lady's fingers tooth oyster breeding line of 1st generation shell set up for 2010, select the individuality 150 possessing this proterties (individuality is large, shell is golden yellow, color is gorgeous) as 2nd generation parent shellfish.

(2) smart ovum obtain, artificial insemination and hatching, larvae cultivation, collect seedling, sea area oyster seed intermediate rearing, seedling takes off the step such as base and monomer cultivation with 1st generation seed selection in oyster.

Within 2011, obtain the sexual maturity of 1st generation breeding line, by embodiment 1, in August, 2012 obtains the sexual maturity of 2nd generation breeding line, successfully selects the stable golden yellow fast lady's fingers tooth oyster strain of shell of the 4th generation proterties to 2013.Compared with control group (non-seed selection group), the long strain of golden yellow shell look speed has the advantages such as growth is fast, shell is golden yellow, color is gorgeous, and its growth rate improves more than 10% than control group.It has higher commodity value, and be suitable for as cultchless oyster cultivation and eat oyster production raw, this has important function for raising Portuguese oyster cultured output, commodity value and economic benefit.

The present invention adopts unexpected mass incident and family selective breeding method, and through the directive breeding in continuous 4 generations, cultivating the stable shell of proterties is golden yellow fast lady's fingers tooth oyster new lines, establishes the breeding method of the golden yellow fast lady's fingers tooth oyster new lines of shell.Above are only a specific embodiment of invention, but design concept of the present invention is not limited thereto, all changes utilizing this design the present invention to be carried out to unsubstantiality, all should belong to the behavior of invading scope.

Claims (3)

1. a breeding method for the golden yellow fast lady's fingers tooth oyster new lines of shell, it comprises the steps:
1) close shellfish is selected: from a large amount of cultured population of Portuguese oyster, select 2 ages more than, and shell is in the individuality golden yellow, color is gorgeous, shell mould is regular; Measure the full weight of all picking individual, before then intercepting full weight, the male and female of 10% are individual as breeding close shellfish;
2) smart ovum obtains: dissect close shellfish, distinguish male and female, carried out respectively by close shellfish good for gonad development washing ovum, washing essence, and with 300 mesh sieve thin,tough silk impurity screenings, ovum is collected in the white bucket of 160L plastics of mark respectively, and seminal fluid is collected in the 500ml beaker of mark respectively;
3) artificial insemination and hatching:
A. mass mating: get male and female parent shellfish each 20-25, its ovum and sperm mixing mating;
B. stud mating: get male and female parent shellfish, carry out male and female individuality 1 to 1 mating, set up family;
The white bucket of 160L plastics each group of fertilized egg being placed in respectively mark is hatched, and seminal concentration is advisable with 3-4 sperm around each ovum, adopts the precipitation method to wash ovum 2-3 time during polyspermism; Triploidy rate 40-50/ml, micro-inflation, hatching water temperature 25-30 DEG C;
4) larvae cultivation: development of fertilized ova is cultivated often organizing larvae collection in three white buckets of 160L plastics marked respectively to adopting siphon method during D type larva, often organizes 3 parallel group; D type larvae cultivation density domination, at 8-12/ml, along with larval growth reduces density gradually, is down to 1-2/ml to Creeping larva density; Change water every day 2 times, change water 30%-50% at every turn, after larva attachment, increase to 60%-100%; D type larva starts chrysophyceae of throwing something and feeding, and daily ration of feeding increases to 2-3 ten thousand cells/ml in mid-term gradually from the 1-2 ten thousand cells/ml at initial stage, later stage 3-4 ten thousand cells/ml, and larvae development adds flat algae to the shell top later stage, and daily ration of feeding is 1-2 ten thousand cells/ml; Suitably throw something and feed during larvae cultivation Chaetoceros or chlorella, water temperature 25-30 DEG C during larvae cultivation, salinity 23-28, pH value 8.0-8.5, need 14-20 days from D type larvae cultivation to Creeping larva; In larvae cultivation process, keep each group of breeding line larval density identical, daily ration, feeding quantity is consistent with rate of water exchange, and breeding condition is identical; Container for plant growth, the utensil of each group of breeding line individually use, and avoid mixing;
5) collect seedling: start to throw in collector when larval growth occurs eyespot to shell long 320-330um, 70-80%; The gray tinted plastic sheet that collector adopts non-toxic material to make, plastic sheet specification is long 20-30cm, wide 1-3cm, thick 0.1-0.3cm; With 60-80 silk polyethylene rope by the plastic sheet string cleaned together, tie up and on cross bar, hang on 60-80cm place, nursery pond underwater collect seedling, every cubic metre of water body throws in plastic sheet 30-40 string; Every sheet plastic sheet spatfall 20-30; Oyster seedling grows to the long 1-2mm of shell, marks each breeding line and move to sea area to carry out seed intermediate rearing;
6) sea area oyster seed intermediate rearing: the collector enclosing oyster seedling of mark is moved to same a slice sea area and cultivates; If meet the natural waters Portuguese oyster larva setting stage, first can move to pond rearing, move to sea area after the phase to be attached again and cultivate; Water temperature 13-26 DEG C, under the condition of salinity 26-30, cultivate through 90-120 days sea areas, oyster seedling can grow to the long 4-5cm of shell;
7) in oyster, seedling takes off base and monomer cultivation: when oyster seedling grows to 4-5cm, bending oyster adherance plastic sheet, and oyster seedling just comes off smoothly, forms cultchless oyster seedling; The cultivation basket that the cultchless oyster seedling peeled off by each breeding line loads mark is hung on the cultivation of same a slice sea area; Periodic cleaning aufwuch during cultivation, and measure growing state;
8) new lines purifying: select to possess individual large, individuality that the golden yellow proterties of shell, shell mould are regular as second generation parent from the golden yellow fast lady's fingers tooth oyster breeding line first generation of shell, the each step of second generation seed selection with first generation selection, until select the golden yellow fast lady's fingers tooth oyster strain of the stable shell of proterties.
2. the breeding method of the golden yellow fast lady's fingers tooth oyster new lines of a kind of shell as claimed in claim 1, is characterized in that: step 5) in, plastic sheet often goes here and there 16-20 sheet, pitch of fins 13-17cm.
3. the breeding method of the golden yellow fast lady's fingers tooth oyster new lines of a kind of shell as claimed in claim 1, is characterized in that: step 5) in, at water temperature 25-30 DEG C, cultivated through 5-6 days after larva set, oyster seedling can grow to the long 1-2mm of shell.
CN201410197671.1A 2014-05-12 2014-05-12 The breeding method of the golden yellow fast lady's fingers tooth oyster new lines of a kind of shell CN103975879B (en)

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CN104365515B (en) * 2014-11-25 2016-07-13 鲁东大学 A kind of selection of the soft body long Concha Ostreae of height melanin content
CN105494177B (en) * 2015-08-24 2018-05-08 福建省水产研究所 A kind of producing method for seed of the long breeding line of Portuguese oyster speed
CN105360022B (en) * 2015-10-13 2017-11-21 中国科学院南海海洋研究所 A kind of producing method for seed of double golden shell Kumamoto oyster strains
CN105360023B (en) * 2015-10-13 2017-12-05 中国科学院南海海洋研究所 A kind of producing method for seed of golden adductor scar Kumamoto oyster strain
CN105165686B (en) * 2015-10-13 2018-02-23 中国科学院南海海洋研究所 A kind of producing method for seed of double black shell Kumamoto oyster strains
CN105900874B (en) * 2016-04-22 2018-08-31 广东海洋大学 A kind of breeding method of the pteria martensii that carotenoid content is high cultivation new lines
CN106069935A (en) * 2016-06-28 2016-11-09 苏州市相城区阳澄湖镇剑美水产生态养殖专业合作社 The cultural method of Concha Ostreae

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