CN102257974A - Artificial ripening acceleration culture and spawning induction method for mytilus coruscus - Google Patents
Artificial ripening acceleration culture and spawning induction method for mytilus coruscus Download PDFInfo
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- CN102257974A CN102257974A CN2011101316116A CN201110131611A CN102257974A CN 102257974 A CN102257974 A CN 102257974A CN 2011101316116 A CN2011101316116 A CN 2011101316116A CN 201110131611 A CN201110131611 A CN 201110131611A CN 102257974 A CN102257974 A CN 102257974A
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- trachyostracous mussel
- shellfish
- seawater
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- hastening parturition
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention provides an artificial ripening acceleration and spawning induction method for mytilus coruscus serving as oceanic shellfish. The method comprises the following steps of: performing reinforcing culture on parent shellfish, accelerating ripening artificially, accelerating spawning artificially and mixing and hatching for culturing. The artificial ripening acceleration culture and spawning induction method for the mytilus coruscus has a great significance for development of aspects of seedling production, sustainable aquaculture and the like of the mytilus coruscus serving as economic shellfish.
Description
Technical field
The shellfish that the present invention relates to aquaculture field is propagated artificially, specifically, relates to the Trachyostracous mussel artificial repening and cultivates and the method for hastening parturition.
Background technology
Trachyostracous mussel is the important mariculture shellfish of China, is distributed in the Huanghai Sea, the Bohai Sea and East China Sea lefteye, and wherein with Zhejiang coastal resources amount maximum, the Shengsi County, Zhoushan is the major production areas of Trachyostracous mussel.For a long time, Trachyostracous mussel is cultured required seed supply and is depended on the sea area semi always.In recent years, because the people is over-exploitation, cause the Trachyostracous mussel natural resources to reduce gradually: natural waters spatfall quality and quantity obviously descends, and the Trachyostracous mussel aquaculture industry is affected.In addition, owing to be subjected to the restriction of mating season, the correlative study of the Trachyostracous mussel settlement and metamorphosis that can carry out every year and Trachyostracous mussel parent's shellfish breeding time have a greatly reduced quality, and are difficult to be protected.Disclose the artificial raise seedling method of Trachyostracous mussel as patent application CN200710156048.1, but do not disclose the method for accelerating the ripening and hastening parturition of Trachyostracous mussel.
Summary of the invention
Technical problem to be solved by this invention is, overcomes the defective of prior art, provides a kind of seashells Trachyostracous mussel artificial repening and the method for hastening parturition.
The present invention realizes purpose of the present invention by the following technical solutions.
The Trachyostracous mussel artificial repening and the method for hastening parturition is characterized in that, comprise the steps:
A. close shellfish hot housing: select Trachyostracous mussel parent shellfish, put into the vessel that fill 12 ℃ of-15 ℃ of seawater after cleaning with seawater, subsequently these vessel are put into incubator and carry out reinforced cultivating;
B. artificial repening: incubator is heated up 0.5-1 ℃ every day,, differentiate the maturity of sexual gland according to the vesicle development degree in the outer embrane, until gonadal maturation until 21-24 ℃;
C. artificial induced spawning: put into incubator after Trachyostracous mussel is sealed in plastic sack, around its sack, fill ice cube, behind the 12h-15h, closeer shellfish is taken out from incubator, the close shellfish of discharging sperm and ovum is isolated respectively, be positioned over 20-22 ℃.
Preferably, in the described steps A, comprise the attachment of removing Trachyostracous mussel parent shellfish body surface, and cut off byssus.
Preferably, in the described steps A, be 3-7 days during the reinforced cultivating, close shellfish density is 3-5/L, changes whole seawater every day, and bait is very thin Chaetoceros or chlorella, and throw something and feed 2-3 every day, and its density of throwing something and feeding is 200 * 10
4-300 * 10
4Cells/ml.
Preferably, among the described step B, if observe the still prematurity of close shellfish, the reinforced cultivating 1 time of heating up then needs to heat up 0.5 ℃ to 21-24 ℃ from 12 ℃-15 ℃ every days again.
Preferably, among the described step B, the artificial repening nurturing period, change once all seawater every day; And throw something and feed every day 2-3 very thin Chaetoceros of bait or chlorella, its density of throwing something and feeding is 300-600 * 10
4Cells/ml.
Preferably, among the described step C that hastens parturition, in the seawater, salinity is 25-30 ‰; In the process of hastening parturition, trace inflation, dark unglazed photograph.
Preferably, described Trachyostracous mussel parent shellfish is regular shape, specification unanimity; Test used seawater through the composite fibre membrane filtration, salinity 30 ‰.
Preferably, behind the described artificial induced spawning, observe sperm viability and ovum shape under inverted microscope, select high-quality sperm and ovum mixing fertilization: behind the fertilization 15-20min, whether microscopically is observed has polar body to occur, polar body occurring above 80% fertilized egg can clean, hatching is cultivated then, and the hatching nurturing period is 2 days, and seawater salinity is 25-30 ‰, dark unglazed photograph does not have inflation.
The present invention has the following advantages:
1. can not be subject to seasonal restrictions, all can carry out accelerating the ripening of Trachyostracous mussel at any time and cultivate and hasten parturition; Allow male and female parent shellfish in different vessel, discharge respectively, can obtain high-quality sperm and ovum, and then obtain high quality fertilized egg of scophthatmus;
2. the Trachyostracous mussel artificial repening cultivation of the present invention and the method for hastening parturition are significant for aspects such as the seedling production of this economic shellfish of development Trachyostracous mussel, sustainable breed;
3. cultivate and the method for hastening parturition by of the present invention accelerating the ripening, can prolong the mating season of Trachyostracous mussel parent shellfish greatly, guarantee that the time about annual 8-10 month can be carried out the aspect research of Trachyostracous mussel young settlement and metamorphosis.
Embodiment
In order to make technological means of the present invention, creation characteristic, to reach purpose and effect is easy to understand, the present invention is further elaborated below in conjunction with embodiment.
Embodiment
The Trachyostracous mussel artificial repening and the method for hastening parturition comprise the steps:
(1) parent of the Trachyostracous mussel after gathering shellfish is screened, the one-tenth shellfish of selected shape rule, specification unanimity, seawater is through the composite fibre membrane filtration, salinity 30 ‰;
(2) will collect good close shellfish, and after removing the body surface attachment and cutting off byssus, put into the tank that fills 13 ℃ of seawater after cleaning with seawater, this tank being put into incubator subsequently carries out reinforced cultivating again;
Be 3-7 days during the reinforced cultivating, close shellfish density is 6/L, changes whole seawater every day, and bait is very thin Chaetoceros or chlorella, throws something and feeds every day 3 times, and its density of throwing something and feeding is 200 * 10
4Cells/ml;
(3) the artificial repening nurturing period, heat up 0.5 ℃ to 21 ℃ every day.Test used seawater through the composite fibre membrane filtration, salinity 30 ‰.The artificial repening nurturing period, change once all seawater every day, throw something and feed every day 2 very thin Chaetoceros of bait and 1 chlorella, its density of throwing something and feeding is 300 * 10
4Cells/ml;
If observe the still prematurity of close shellfish, the reinforced cultivating 1 time of heating up then needs to heat up 0.5 ℃ to 21 ℃ from 13 ℃ of every days again;
(4) will fill Trachyostracous mussel sealed bag and ice cube and be positioned over earlier, behind the 12h, closeer shellfish be taken out from incubator, isolate being positioned in 21 ℃ of seawater respectively separately at incubator; In the process of hastening parturition, trace inflation, dark unglazed photograph;
(5) under inverted microscope, observe sperm viability and ovum shape, select very fast sperm and the perfectly round full ovum mixing fertilization of trip speed, clean fertilized egg behind the 15min, be positioned over hatching cultivation in the incubator, the cultivation time is 2 days, and temperature is 17 ℃, seawater salinity 30 ‰, dark unglazed photograph does not have inflation.
Cultivate and the method for hastening parturition by Trachyostracous mussel artificial repening of the present invention, broken through the restriction of mating season in the past, obtained high-quality Trachyostracous mussel fertilized egg, incubation rate surpasses 80%, has obtained the D shape young that can cultivate.
More than show and described basic principle of the present invention, principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; that describes in the foregoing description and the specification just illustrates principle of the present invention; the present invention also has various changes and modifications without departing from the spirit and scope of the present invention, and these changes and improvements all fall in the claimed scope of the invention.
Claims (8)
1. the Trachyostracous mussel artificial repening and the method for hastening parturition is characterized in that, comprise the steps:
A. close shellfish hot housing: select Trachyostracous mussel parent shellfish, put into the vessel that fill 12 ℃ of-15 ℃ of seawater after cleaning with seawater, subsequently these vessel are put into incubator and carry out reinforced cultivating;
B. artificial repening: incubator is heated up 0.5-1 ℃ every day,, differentiate the maturity of sexual gland according to the vesicle development degree in the outer embrane, until gonadal maturation until 21-24 ℃;
C. artificial induced spawning: put into incubator after Trachyostracous mussel is sealed in plastic sack, around its sack, fill ice cube, behind the 12h-15h, closeer shellfish is taken out from incubator, the close shellfish of discharging sperm and ovum is isolated respectively, be positioned over 20-22 ℃.
2. the Trachyostracous mussel artificial repening according to claim 1 and the method for hastening parturition is characterized in that, in the described steps A, comprise the attachment of removing Trachyostracous mussel parent shellfish body surface, and cut off byssus.
3. the Trachyostracous mussel artificial repening according to claim 1 and the method for hastening parturition, it is characterized in that, in the described steps A, be 3-7 days during the reinforced cultivating, parent shellfish density is 3-5/L, changes whole seawater every day, and bait is very thin Chaetoceros or chlorella, throw something and feed 2-3 every day, and its density of throwing something and feeding is 200 * 10
4-300 * 10
4Cells/ml.
4. the Trachyostracous mussel artificial repening according to claim 1 and the method for hastening parturition is characterized in that, among the described step B, if observe the still prematurity of close shellfish, the reinforced cultivating 1 time of heating up then needs to heat up 0.5 ℃ to 21-24 ℃ from 12 ℃-15 ℃ every days again.
5. the Trachyostracous mussel artificial repening according to claim 1 and the method for hastening parturition is characterized in that, among the described step B, the artificial repening nurturing period, change once all seawater every day; And throw something and feed every day 2-3 very thin Chaetoceros of bait or chlorella, its density of throwing something and feeding is 300-600 * 10
4Cells/ml.
6. the Trachyostracous mussel artificial repening according to claim 1 and the method for hastening parturition is characterized in that among the described step C that hastens parturition, in the seawater, salinity is 25-30 ‰; In the process of hastening parturition, trace inflation, dark unglazed photograph.
7. the Trachyostracous mussel artificial repening according to claim 1 and the method for hastening parturition is characterized in that, described Trachyostracous mussel parent shellfish is regular shape, specification unanimity; Test used seawater through the composite fibre membrane filtration, salinity 30 ‰.
8. the Trachyostracous mussel artificial repening according to claim 1 and the method for hastening parturition, it is characterized in that, behind the described artificial induced spawning, under inverted microscope, observe sperm viability and ovum shape, select high-quality sperm and ovum mixing fertilization: behind the fertilization 15-20min, whether microscopically is observed has polar body to occur, and polar body occurring above 80% fertilized egg can clean, and hatching is cultivated then, the hatching nurturing period is 2 days, seawater salinity is 25-30 ‰, and dark unglazed photograph does not have inflation.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011101316116A CN102257974A (en) | 2011-05-19 | 2011-05-19 | Artificial ripening acceleration culture and spawning induction method for mytilus coruscus |
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| CN2011101316116A CN102257974A (en) | 2011-05-19 | 2011-05-19 | Artificial ripening acceleration culture and spawning induction method for mytilus coruscus |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919169A (en) * | 2012-10-19 | 2013-02-13 | 中国科学院海洋研究所 | Device and method for cultivating atrina pectinata parent shellfish |
| CN103210856A (en) * | 2013-03-29 | 2013-07-24 | 上海海洋大学 | Application of ethanol to regulating and controlling mytilus coruscus larva metamorphosis |
| CN103548720A (en) * | 2013-10-17 | 2014-02-05 | 苏州市阳澄湖渔业科技中心有限公司 | Method for artificial oxytocin and fertilization of Mytilus coruscus |
| CN105104266A (en) * | 2015-08-28 | 2015-12-02 | 上海海洋大学 | Artificial spawning and cultivation method for mytilus coruscus |
| CN105265352A (en) * | 2014-07-23 | 2016-01-27 | 北海市铁山港区生产力促进中心 | Seawater pearl culture nucleus-inserting bead-breeding spawning induction sperm-and-egg-emission method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101317549A (en) * | 2007-10-10 | 2008-12-10 | 浙江海洋学院 | Artificial nursery stock growing method for thick-shell mussel |
-
2011
- 2011-05-19 CN CN2011101316116A patent/CN102257974A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101317549A (en) * | 2007-10-10 | 2008-12-10 | 浙江海洋学院 | Artificial nursery stock growing method for thick-shell mussel |
Non-Patent Citations (3)
| Title |
|---|
| 《南方水产》 20070630 常抗美 厚壳贻贝人工繁殖技术的研究 26-30 1-8 第3卷, 第3期 * |
| 《科学养鱼》 20080530 陈清建 厚壳贻贝人工育苗技术研究 26 1-8 , * |
| 《齐鲁渔业》 20051231 祝世军 厚壳贻贝人工育苗技术研究 28-29 1-8 第22卷, 第9期 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919169A (en) * | 2012-10-19 | 2013-02-13 | 中国科学院海洋研究所 | Device and method for cultivating atrina pectinata parent shellfish |
| CN102919169B (en) * | 2012-10-19 | 2015-02-25 | 中国科学院海洋研究所 | Device and method for cultivating atrina pectinata parent shellfish |
| CN103210856A (en) * | 2013-03-29 | 2013-07-24 | 上海海洋大学 | Application of ethanol to regulating and controlling mytilus coruscus larva metamorphosis |
| CN103548720A (en) * | 2013-10-17 | 2014-02-05 | 苏州市阳澄湖渔业科技中心有限公司 | Method for artificial oxytocin and fertilization of Mytilus coruscus |
| CN103548720B (en) * | 2013-10-17 | 2016-04-06 | 苏州市阳澄湖渔业科技中心有限公司 | A kind of method of Trachyostracous mussel artificial induced spawning and insemination |
| CN105265352A (en) * | 2014-07-23 | 2016-01-27 | 北海市铁山港区生产力促进中心 | Seawater pearl culture nucleus-inserting bead-breeding spawning induction sperm-and-egg-emission method |
| CN105104266A (en) * | 2015-08-28 | 2015-12-02 | 上海海洋大学 | Artificial spawning and cultivation method for mytilus coruscus |
| CN105104266B (en) * | 2015-08-28 | 2018-06-08 | 上海海洋大学 | A kind of Trachyostracous mussel artificial induced spawning and the method for cultivation |
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Application publication date: 20111130 |