CN103766258A - Artificial breeding method of Ruditapes variegata - Google Patents
Artificial breeding method of Ruditapes variegata Download PDFInfo
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- CN103766258A CN103766258A CN201410036946.3A CN201410036946A CN103766258A CN 103766258 A CN103766258 A CN 103766258A CN 201410036946 A CN201410036946 A CN 201410036946A CN 103766258 A CN103766258 A CN 103766258A
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- ruditapes
- larva
- variegated clam
- variegata
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses an artificial breeding method of Ruditapes variegata, and a large of healthy offspring seeds are cultured indoors by the links of parent sample collection, gonad maturation acceleration, parturition hastening and incubation, larva cultivation, substratum-free offspring seed collection, juvenile mollusk breeding and the like. The awkward situation that R. philippinarum is mistaken for the Ruditapes variegata by most people in the prior art, and the real artificial breeding of the Ruditapes variegata does not exist is over, the healthy offspring seeds of the Ruditapes variegata are cultured for the first time, and a solid foundation is established for recovering the biological resources of the Ruditapes variegata and maintaining the genetic polymorphism of the Ruditapes variegata. The artificial breeding method has the advantages of simpleness in operation, strong practicability, easiness in popularization and the like.
Description
Technical field
The present invention relates to shellfish seed breeding technical field in modern marine agricultural, particularly a kind of variegated clam
ruditapes variegataartificial raise seedling method.
Background technology
Variegated clam
ruditapes variegata, to be that clam is young belong to (
ruditapes Chiamenti, 1990), sew bright and beautiful clam subfamily (
tapetinae H. & A. Adams) Veneridae (
veneridae Refihesque, 1815) a kind of intertidal shellfish, with Ruditapes philippinarum
ruditapes philippinarumtwo species (Zhuan Qiqian, 2000) of same subordinate.
Variegated clam
ruditapes variegatabe distributed on the south Pingtan, China Fujian, the ground such as Guangdong, Guangxi, Hainan, hobby hot environment, the wild amount of catching of adopting is very limited, is accompanied by industrial pollution, beach atrophy etc. and causes this species resource very rare; And Ruditapes philippinarum
ruditapes philippinarumthat to be distributed in China north and south coastal, the wide salt of wide temperature, especially in Fujian, Shandong, Liaoning cultivation is more, annual production, at more than 200 ten thousand tons, is the marine economy shellfish that China's per unit area yield is the highest.
In China, Ruditapes philippinarum
ruditapes philippinarumquantity is huge, by people common, because Ruditapes philippinarum shell face look changeful is, colorful, thus a lot of people by appearance all by Ruditapes philippinarum
ruditapes philippinarumregard as " variegated clam ", this has just caused variegated clam
ruditapes variegataitself is mistaken for Ruditapes philippinarum.And maximum difference is variegated clam between these two kinds of clams
ruditapes variegatawater pipe is free from base portion, and free length of pipe and water pipe total length are almost equal; And Ruditapes philippinarum
ruditapes philippinarumwater pipe is only free at top, and the 20%(that free length is no more than water pipe total length opens state model & Yan and likes force, 2010).
So far, the variegated clam of having reported
ruditapes variegatapaper, the patent etc. of artificial breeding aspect is all to Ruditapes philippinarum
ruditapes philippinarum, in fact, neither one report sampling parent is variegated clam
ruditapes variegata.Through having consulted domestic and foreign literature, find all reports that have about variegated clam artificial breeding, all Ruditapes philippinarum is mistaken for to variegated clam and reports (Mao Xueying, 2003; Yang Yuxiang etc., 2005; Shi Bin etc., 2005,2006; Kan Jiandong etc., 2006; Open woods medium, 2012).The variegated clam Tu Chi of Yan Zhihong etc. (2003) the report research of collecting seedling, is actually the native pond collection technique of Ruditapes philippinarum, because Putian, fujian Province is maximum Ruditapes philippinarum seed base, the whole nation, and national more than 30% seed of supply.The parental source of all documents is all to the north of Pingtan, Fujian above, and these areas just do not have variegated clam distribute (Pan Heting etc., 2008 at all; Liu is congruence mutually, and 2011).
Therefore in fact also there is no a kind of variegated clam
ruditapes variegataartificial breeding method's report.
Summary of the invention
The present invention is in order to recover the variegated clam of China native country scarce resource, safeguards the bio-diversity of its species, proposes the artificial breeding method of a kind of variegated clam.
Technical solution of the present invention is: the artificial breeding method of a kind of variegated clam, is characterized in that: described method is carried out according to following steps:
A, on the south Fujian China Pingtan, carry out variegated clam parent gather, do not have fully-developed individuality to carry out accelerating processing for sexual gland, the individuality of gonad maturity can directly use, accelerating processing procedure is that variegated clam is placed in indoor cement pit, adopt micro-inflation, manually the throw something and feed mode of Micro Algae is carried out accelerating
B, be positioned in seawater after variegated clam parent is rinsed well, adopt the 0.5-3h that dries in the shade to hasten parturition with the way that flowing water 30-60min combines, the ripe gamete obtaining is inflated to hatching, hatching density be 30-50 individual/ml,
C, adopt 300 mesh sieve tulles to carry out preferably to the D type larva hatching, carry out larva cultivation afterwards according to the density of 2-3/ml, Miao Chijun all between larva culture period do not change water, adopt micro-airing form to cultivate,
D, when larval growth is to 210-240 μ m, while going out the former base of the gill, control its larval density and carry out collecting seedling without adherance in the scope of 0.5-1/ml, then continue to cultivate larva 3-9d,
E, at the bottom of more than 90% larva is attached to culturing pool time, carry out being bred as in the middle of indoor juvenile mollusk, micro-inflation in incubation process in the middle of juvenile mollusk, do not change water, using shrimp pool water as biological feed, through the cultivation of 30-45d, obtain the variegated clam juvenile mollusk seed of 2-5mm.
Compared with the existing technology, tool has the following advantages in the present invention:
Variegated clam artificial breeding method disclosed in this invention, the most people that is through with is traditionally by Ruditapes philippinarum
ruditapes philippinarumbe mistaken for variegated clam, there is no the truly awkward situation of variegated clam artificial breeding, use a series of science, simple operating procedure to cultivate first variegated clam healthy seedling, for its living resources are recovered and maintain its genetic polymorphism to have established solid foundation.That the present invention has is simple to operate, practical, be easy to the advantages such as popularization.
Accompanying drawing explanation
Fig. 1 is variegated, and clam fingerlings artificial is bred schematic flow sheet;
A: variegated clam (water pipe base portion is free); B: Ruditapes philippinarum (water pipe top is free); C: mid-term veliger; D: the later stage veliger; E: just completed abnormal juvenile mollusk; F: two water pipe juvenile mollusks.
Embodiment
Below in conjunction with accompanying drawing 1, the specific embodiment of the present invention is described.
Embodiment
On July 12nd, 2013, collect variegated clam sample at Zhanjiang Naozhou Island of Zhanjiang, in order to ensure species unicity.Be positioned in the cement pit of permanent emerging southern marine organisms Science and Technology Ltd. nursery, leave standstill a moment, after its water pipe stretches out, catch the individuality that all water pipes start to dissociate from base portion, reject and do not stretch out water pipe and water pipe starts free Ruditapes philippinarum from top, what remain is variegated clam sample.。
Filter out shell length the pure variegated clam of 3.0-3.6cm individual 2000 as parent.Be positioned in indoor cement pit, adopted micro-inflation, manually the throw something and feed mode of Micro Algae is carried out accelerating.Because this time is in variegated clam mating season, so the sexual gland of variegated clam own is just fine, but also do not have ripe good, after the raising of 8d, variegated clam gonad maturity.
July 20, get at random 500 parents and rinse well, after the surface contaminants that cleans with a brush, be positioned in seawater, adopt the 1.5h that dries in the shade to hasten parturition with the way that flowing water 45min combines, obtain approximately 2.1 hundred million mature eggs that naturally discharge.From laying eggs, approximately, through the 40min end of laying eggs, close shellfish is removed.Inflation hatching fertilized egg, hatching density is controlled at 30-50/ml.
The D shape larva hatching is utilized to siphon method, adopt 300 mesh sieve tulles to concentrate, select altogether larva 1.8 hundred million larvas, be distributed in 6 12m
3cement pit in, cultivate density be 2.5/ml.Nurturing period, all Miao Chijun do not change water, adopt micro-airing form to cultivate; Bait is take cloud tail algae and yeast as main, and the standard of throwing something and feeding is repeatedly to throw less, keeps water quality pure and fresh.During this period, water temperature is at 27.8-29.6 ℃, and salinity is at 25-28ppt.
Through the cultivation of 12-15d, when larval growth is during to 210-240 μ m, while there is the former base of the gill, using at the bottom of pond as adherance, control its density 0.6-0.8/ml, carry out collecting seedling without adherance.Continue to cultivate 7d according to larvae cultivation mode, 90% above larva all adheres to complete, in the middle of Yuan Chizhong carries out juvenile mollusk, is bred as.
After larva adheres to, carry out being bred as in the middle of indoor juvenile mollusk.During this period, micro-inflation, do not change water; Using shrimp pool water as biological feed, the standard of throwing something and feeding keeps water colour slightly general green, makes its juvenile mollusk always in feeding state, constantly growth.Cultivate through 40d, obtain the health variegated clam juvenile mollusk seed of 1,500 ten thousand shell length at 2-5mm.
Claims (1)
1. a variegated clam
ruditapes variegataartificial breeding method, it is characterized in that: described method is carried out according to following steps:
A. the parent who carries out variegated clam on the south Fujian China Pingtan gathers, do not have fully-developed individuality to carry out accelerating processing for sexual gland, the individuality of gonad maturity can directly use, accelerating processing procedure is that variegated clam is placed in indoor cement pit, adopt micro-inflation, manually the throw something and feed mode of Micro Algae is carried out accelerating
B. after variegated clam parent being rinsed well, be positioned in seawater, adopt the 0.5-3h that dries in the shade to hasten parturition with the way that flowing water 30-60min combines, the ripe gamete obtaining is inflated to hatching, hatching density is 30-50/ml,
C. adopt 300 mesh sieve tulles to carry out preferably to the D type larva hatching, carry out larva cultivation afterwards according to the density of 2-3/ml, Miao Chijun all between larva culture period do not change water, adopt micro-airing form to cultivate,
D. when larval growth is to 210-240 μ m, while going out the former base of the gill, control its larval density and carry out collecting seedling without adherance in the scope of 0.5-1/ml, then continue to cultivate larva 3-9d,
E. at the bottom of more than 90% larva is attached to culturing pool time, carry out being bred as in the middle of indoor juvenile mollusk, micro-inflation in incubation process in the middle of juvenile mollusk, do not change water, using shrimp pool water as biological feed, through the cultivation of 30-45d, obtain the variegated clam juvenile mollusk seed of 2-5mm.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106106272A (en) * | 2016-06-20 | 2016-11-16 | 中国科学院南海海洋研究所 | A kind of Che oyster artificial breeding method |
| CN106165659A (en) * | 2016-06-20 | 2016-11-30 | 中国科学院南海海洋研究所 | A kind of Concha Tridacna squamosa artificial breeding method |
| CN106719184A (en) * | 2016-12-29 | 2017-05-31 | 丹东市水产技术推广总站 | A kind of artificial breeding method of Mactra chinensis |
| CN111183937A (en) * | 2020-03-04 | 2020-05-22 | 大连海洋大学 | Artificial breeding method for improving yield of Ruditapes philippinarum |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101433187A (en) * | 2008-12-17 | 2009-05-20 | 大连水产学院 | Artificial breeding method of Mactra chinensis |
| KR20130053475A (en) * | 2011-11-14 | 2013-05-24 | 전라남도 | Mass production method of sinonovacula constricta's artificial seeds |
-
2014
- 2014-01-26 CN CN201410036946.3A patent/CN103766258B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101433187A (en) * | 2008-12-17 | 2009-05-20 | 大连水产学院 | Artificial breeding method of Mactra chinensis |
| KR20130053475A (en) * | 2011-11-14 | 2013-05-24 | 전라남도 | Mass production method of sinonovacula constricta's artificial seeds |
Non-Patent Citations (5)
| Title |
|---|
| 于守团等: "菲律宾蛤仔升温人工育苗技术", 《齐鲁渔业》 * |
| 周素琴等: "青蛤生产性育苗及中间培育技术研究", 《浙江海洋学院学报(自然科学版)》 * |
| 石斌等: "杂色蛤工厂化人工育苗技术", 《齐鲁渔业》 * |
| 郭文等: "菲律宾蛤仔室内常温育苗技术", 《齐鲁渔业》 * |
| 阚建东等: "杂色蛤人工育苗试验", 《河北渔业》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106106272A (en) * | 2016-06-20 | 2016-11-16 | 中国科学院南海海洋研究所 | A kind of Che oyster artificial breeding method |
| CN106165659A (en) * | 2016-06-20 | 2016-11-30 | 中国科学院南海海洋研究所 | A kind of Concha Tridacna squamosa artificial breeding method |
| CN106106272B (en) * | 2016-06-20 | 2018-11-23 | 中国科学院南海海洋研究所 | A kind of Che oyster artificial breeding method |
| CN106165659B (en) * | 2016-06-20 | 2018-11-30 | 中国科学院南海海洋研究所 | A kind of giant clam artificial breeding method |
| CN106719184A (en) * | 2016-12-29 | 2017-05-31 | 丹东市水产技术推广总站 | A kind of artificial breeding method of Mactra chinensis |
| CN111183937A (en) * | 2020-03-04 | 2020-05-22 | 大连海洋大学 | Artificial breeding method for improving yield of Ruditapes philippinarum |
| CN111183937B (en) * | 2020-03-04 | 2022-02-01 | 大连海洋大学 | Artificial breeding method for improving yield of Ruditapes philippinarum |
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