CN111183937B - Artificial breeding method for improving yield of Ruditapes philippinarum - Google Patents
Artificial breeding method for improving yield of Ruditapes philippinarum Download PDFInfo
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- CN111183937B CN111183937B CN202010143990.XA CN202010143990A CN111183937B CN 111183937 B CN111183937 B CN 111183937B CN 202010143990 A CN202010143990 A CN 202010143990A CN 111183937 B CN111183937 B CN 111183937B
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- 238000009395 breeding Methods 0.000 title claims abstract description 55
- 241000620877 Ruditapes philippinarum Species 0.000 title claims abstract description 51
- 230000001488 breeding effect Effects 0.000 claims abstract description 36
- 238000000034 method Methods 0.000 claims abstract description 36
- 230000026109 gonad development Effects 0.000 claims abstract description 23
- 230000005070 ripening Effects 0.000 claims abstract description 21
- 235000015170 shellfish Nutrition 0.000 claims abstract description 15
- 238000007689 inspection Methods 0.000 claims abstract description 13
- 230000032696 parturition Effects 0.000 claims abstract description 12
- 239000000178 monomer Substances 0.000 claims abstract description 11
- 238000012258 culturing Methods 0.000 claims abstract description 10
- 210000002149 gonad Anatomy 0.000 claims abstract description 9
- 238000004140 cleaning Methods 0.000 claims abstract description 6
- 238000005070 sampling Methods 0.000 claims abstract description 6
- 230000001954 sterilising effect Effects 0.000 claims abstract description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 6
- 238000005728 strengthening Methods 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 55
- 230000008569 process Effects 0.000 claims description 22
- 238000007599 discharging Methods 0.000 claims description 9
- 150000003839 salts Chemical class 0.000 claims description 7
- 238000005273 aeration Methods 0.000 claims description 6
- 230000003203 everyday effect Effects 0.000 claims description 6
- 238000011534 incubation Methods 0.000 claims description 6
- 239000002689 soil Substances 0.000 claims description 6
- 210000003608 fece Anatomy 0.000 claims description 5
- 239000010871 livestock manure Substances 0.000 claims description 5
- 230000000249 desinfective effect Effects 0.000 claims description 4
- 238000002347 injection Methods 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- 241000195493 Cryptophyta Species 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 239000003337 fertilizer Substances 0.000 claims description 3
- 239000012286 potassium permanganate Substances 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 238000005507 spraying Methods 0.000 claims description 3
- 239000004575 stone Substances 0.000 claims description 3
- 239000002352 surface water Substances 0.000 claims description 3
- 241000287828 Gallus gallus Species 0.000 claims description 2
- 235000010469 Glycine max Nutrition 0.000 claims description 2
- 244000068988 Glycine max Species 0.000 claims description 2
- 238000004061 bleaching Methods 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 claims description 2
- 238000002224 dissection Methods 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 235000013336 milk Nutrition 0.000 claims description 2
- 239000008267 milk Substances 0.000 claims description 2
- 210000004080 milk Anatomy 0.000 claims description 2
- 230000000050 nutritive effect Effects 0.000 claims description 2
- 230000001590 oxidative effect Effects 0.000 claims description 2
- 210000000582 semen Anatomy 0.000 claims description 2
- 239000010802 sludge Substances 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims description 2
- 241000238557 Decapoda Species 0.000 claims 1
- 230000037237 body shape Effects 0.000 abstract description 3
- 230000006872 improvement Effects 0.000 description 8
- 230000008901 benefit Effects 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 241000237519 Bivalvia Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
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- 238000006731 degradation reaction Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
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- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses an artificial breeding method for improving the yield of Ruditapes philippinarum, which comprises the following steps: selecting parents, and performing intensive treatment and intensive culture on the parents; carrying out high-temperature insolation sterilization, dredging and cleaning on the seedling raising pond; carrying out ripening on parents with immature gonads, and then stocking; hasten parturition of the parents in the seedling culture pond; larva breeding; selecting Ruditapes philippinarum larvae in the nursery pond; culturing young shellfish and breeding the young shellfish to obtain Ruditapes philippinarum offspring seed; the method of the invention can effectively improve the quality of the parent by selecting the monomer with the shell width and body length ratio arranged at the top 10% as the parent and carrying out strengthening treatment and intensive culture on the parent, and can ensure that the artificial breeding stability is high, the quality of the parent directly influences the quality of bred offspring by carrying out sampling inspection on the gonad development maturity of the parent and carrying out induced spawning according to the inspection result, and can ensure that the Ruditapes philippinarum with stable monomer gram weight and body shape is bred.
Description
Technical Field
The invention relates to the technical field of Ruditapes philippinarum breeding, in particular to an artificial breeding method for improving Ruditapes philippinarum yield.
Background
Ruditapesphippopherarium (Ruditapesphippopherarium) is a wide-temperature and wide-salt species, is mainly distributed on the coasts of the Pacific ocean and the Indian ocean, is used for culturing shellfish globally, has the annual yield of about 378 ten thousand tons in the world, accounts for about 23.5 percent of the aquaculture yield of aquatic animals of the bivalvia, wherein about 80 percent of the aquaculture yield is from the mainland of China, and has wide market prospect;
at present, the ruditapes philippinarum in China has unstable yield, poor stress resistance and annual degradation of economic characters, the development of the ruditapes philippinarum breeding industry is restricted, and the cultivation of a new high-yield stress-resistant ruditapes philippinarum variety is a main way for solving the existing problems, so that the invention provides an artificial breeding method for improving the yield of ruditapes philippinarum, and the defects in the prior art are overcome.
Disclosure of Invention
Aiming at the problems, the invention provides an artificial breeding method for improving the yield of Ruditapes philippinarum, which can effectively improve the quality of parents by selecting the monomers with the shell width-body length ratio arranged at the top 10% as the parents in the seed selection process and carrying out strengthening treatment and intensive culture on the parents, and can ensure that the Ruditapes philippinarum with stable monomer gram weight and body form can be bred by carrying out sampling inspection on the gonad development maturity of the parents and hastening parturition according to the inspection result, thereby ensuring high stability of artificial breeding, and directly influencing the quality of bred offspring by the quality of the parents.
The invention provides an artificial breeding method for improving the yield of Ruditapes philippinarum, which comprises the following steps:
the method comprises the following steps: selecting seeds, selecting a monomer with the ratio of the shell width to the body length arranged at the first 10% in a natural breeding or artificial breeding group as a parent, then carrying out strengthening treatment on the parent, specifically, disinfecting the parent by adopting a potassium permanganate aqueous solution, then putting the parent into a pre-culture pond for fine culture, specifically, feeding 0.5-1.0 mg/L of nutritive salt, soybean milk or shellfish compound feed, and hanging fermented and matured pig manure or chicken manure in the pre-culture pond with the dosage of 50 kg/mu of fertilizer water to ensure that the pond water is yellow brown;
step two: pretreating the seedling raising pool, namely, exposing the seedling raising pool to high temperature for sterilization, dredging and cleaning, and then constructing a seedling raising environment system in the seedling raising pool;
step three: ripening and stocking, screening the developmental maturity of the parents, directly stocking the parents with mature gonads to a nursery pond, ripening the parents with immature gonads firstly and then stocking, after ripening, randomly selecting 200 parents with 100 plus materials for sampling and dissection, and checking the gonad development degree;
step four: hastening parturition, when the gonad development degree inspection result in the third step shows that the parent gonad development degree is higher than 90 percent and the number of parent individuals exceeds 85 percent of the total number, hastening parturition on the parent in the seedling culture pond, discharging surface water in the seedling culture pond, and discharging redundant ruditapes philippinarum semen and excrement in the seedling culture pond;
step five: larva breeding, namely injecting a new water source into the seedling raising pool every day, ensuring that the water level in the seedling raising pool rises by 5-10cm every day, and ensuring that the transparency of water quality in the seedling raising pool is 40-50 cm;
step six: optimizing the larvae, namely optimizing Ruditapes philippinarum larvae in a nursery pond by using a 300-mesh silk net, and then culturing the Ruditapes philippinarum larvae according to the density of 2-3/ml;
step seven: when the body length of the Ruditapes philippinarum larvae reaches 200-;
step eight: when more than 90% of Ruditapes philippinarum young shellfish is attached to the bottom of the breeding pond, performing intermediate breeding of indoor young shellfish, performing micro-inflation in the intermediate breeding process of the young shellfish, and culturing for 30-45 days by using the pond water as biological bait without changing water.
The further improvement lies in that: in the seed selection process in the first step, the surface of the selected parent shell is ensured not to be damaged, and the single gram weight error between parents is controlled to be less than 1.5 g.
The further improvement lies in that: and in the step two, the specific processes of high-temperature insolation sterilization, desilting and cleaning are carried out on the nursery pond: the method comprises the steps of exposing a seedling raising pool at high temperature for 15-20 days until soil on the surface of the pool bottom is cracked, removing black and odorous sludge on the pool bottom, removing shells, stones, gravels and other sundries on the pool bottom, turning over unoxidized pool soil on the pool bottom, fully oxidizing, injecting water into the seedling raising pool to soak the pool until the pool bottom is flat, spraying bleaching water into the seedling raising pool to soak and sterilize for 2-3 days, discharging water in the seedling raising pool, and repeatedly washing with clean water for 2-3 times.
The further improvement lies in that: the specific process of constructing the seedling environment system in the seedling raising pond in the step two is as follows: after the nursery pond is cleaned, the sterilized silt is laid at the bottom of the nursery pond, the laying thickness is 30-40cm, the silt is laid in 3-4 layers, the light salt water is sprayed once every layer of silt is laid, then water is injected into the nursery pond, and the water depth is controlled to be 0.8-1.5 m.
The further improvement lies in that: the process of ripening the parents with immature gonads in the third step is as follows: ripening is accelerated by micro-aeration and artificial feeding of unicellular algae.
The further improvement lies in that: the specific method for hastening parturition in the fourth step comprises the following steps: performing induced spawning by a method of combining drying in the shade for 0.5-3h and flowing water for 30-60min, and then performing air-inflation incubation on the obtained mature Ruditapes philippinarum sperms and Ruditapes philippinarum ova, wherein the incubation density is 30-50/ml.
The further improvement lies in that: and in the fourth step, when the gonad development degree inspection result in the third step shows that the number of parent individuals with parent gonad development degree higher than 90% is lower than 85% of the total number, the ripening treatment in the third step is continued until the parent gonad development course is higher than the spawning promotion requirement.
The further improvement lies in that: and fifthly, when a new water source is injected into the seedling culture pond, the new water source needs to be filtered by a filter screen and injected slowly in the injection process.
The further improvement lies in that: and in the sixth step, the water in the nursery pond is not changed during the culture of the Ruditapes philippinarum larvae, and the culture is carried out in a micro-aeration mode.
The invention has the beneficial effects that: the method has the advantages that the monomer with the shell width and body length ratio arranged at the first 10% is selected as the parent in the seed selection process, the surface of the shell of the parent is not damaged, the single gram weight error between the parents is controlled to be smaller than 1.5g, the parent is subjected to intensive treatment and intensive culture, the quality of the parent can be effectively improved, the gonad development maturity of the parent is subjected to sampling inspection, spawning is induced according to the inspection result, the stability of artificial breeding is high, the quality of bred offspring is directly influenced by the quality of the parent, ruditapes philippinarum with stable monomer gram weight and body shape can be bred, the breeding yield can be improved by pretreating a breeding pond and carrying out ripening and spawning induction on the parent, the stress resistance of the artificially bred ruditapes philippinarum is high, and the economic benefit brought by artificial breeding can be improved.
Detailed Description
In order to further understand the present invention, the following detailed description will be made with reference to the following examples, which are only used for explaining the present invention and are not to be construed as limiting the scope of the present invention.
An artificial breeding method for improving the yield of Ruditapes philippinarum comprises the following steps:
the method comprises the following steps: selecting seeds, selecting a monomer with the ratio of the shell width to the body length arranged in the first 10% in a natural breeding or artificial breeding group as a parent, ensuring that the surface of the shell of the selected parent is not damaged, controlling the single gram weight error between the parents to be less than 1.5g, then carrying out strengthening treatment on the parent, specifically, disinfecting the parent by adopting a potassium permanganate aqueous solution, then putting the parent into a pre-culture pond for intensive culture, specifically feeding nutrient salt of 0.8mg/L, specifically nitrogen, phosphorus, iron or silicon nutrient salt, and hanging fermented and rotten pig manure in the pre-culture pond with the dosage of 50 kg/mu of fertilizer water to ensure that the pond water is tawny;
step two: pretreating a seedling raising pond, namely, exposing the seedling raising pond to high temperature for sterilization, dredging and cleaning, and specifically comprises the following steps: carry out the high temperature insolate 18 days with the pond of growing seedlings till pond bottom surface soil is cracked, then push away the black smelly silt of pond bottom, and the shell with the pond bottom, the stone, debris such as grit clear away, plough the unoxidized pond soil of pond bottom, then fully oxidize, carry out the water injection pond of soaking the pond of growing seedlings again, it levels to the pond bottom, then to watering in the pond of growing seedlings and soak 2 days of disinfecting, the water in the pond of growing seedlings of discharging at last, wash 2 times with the clear water repeatedly, then establish the environment system of growing seedlings in the pond of growing seedlings again, the concrete process of establishing the environment system of growing seedlings in the pond of growing seedlings is: after the nursery pond is cleaned, laying the sterilized silt to the bottom of the nursery pond, wherein the laying thickness is 40cm, laying the silt in 4 layers, spraying light salt water once when each layer of silt is laid, and injecting water into the nursery pond, wherein the water depth is controlled to be 1.2 m;
step three: ripening and stocking, screening the developmental maturity of the parents, directly stocking the parents with mature gonads to a nursery pond, ripening the immature gonads firstly and then stocking, after ripening, randomly selecting 150 parents to sample and dissect, checking the gonad development degree, and the process of ripening the immature gonads comprises the following steps: ripening is accelerated by adopting a micro-aeration and artificial feeding of unicellular algae;
step four: hastening parturition, when the gonad development degree inspection result in the step three shows that the individual number of the parent with parent gonad development degree higher than 90% exceeds 85% of the total number, hastening parturition on the parent in the seedling pool, when the gonad development degree inspection result in the step three shows that the individual number of the parent with parent gonad development degree higher than 90% is lower than 85% of the total number, continuing to perform ripening treatment in the step three until the parent gonad development process is higher than the requirement of hastening parturition, then discharging surface water in the seedling pool, and discharging redundant philippine clam sperm and excrement in the seedling pool, wherein the specific method for hastening parturition comprises the following steps: performing induced spawning by a method of combining drying in the shade for 2 hours and flowing water for 50 minutes, and then performing air inflation incubation on the obtained mature Ruditapes philippinarum sperms and Ruditapes philippinarum ova, wherein the incubation density is 40/ml;
step five: larva cultivation, namely injecting a new water source into the seedling raising pond every day, and when the new water source is injected into the seedling raising pond, filtering the new water source through a filter screen in the injection process, slowing down and slowly injecting the new water source, so that the water level in the seedling raising pond rises by 8cm every day, and the transparency of the water quality in the seedling raising pond is ensured to be 45 cm;
step six: optimizing the larvae, namely optimizing Ruditapes philippinarum larvae in a nursery pond by using a 300-mesh silk net, and then culturing the Ruditapes philippinarum larvae according to the density of 2.5/ml, wherein the nursery pond does not change water during the culture period of the Ruditapes philippinarum larvae, and culturing in a micro-aeration mode;
step seven: when the body length of the Ruditapes philippinarum larvae reaches 220 μm, controlling the larva breeding density of the Ruditapes philippinarum larvae to be 0.8/ml for larva breeding, controlling the water temperature in a larva breeding pool to be 22.5 ℃ and the salinity to be 26 during larva breeding, and periodically checking and adjusting the larva breeding density;
step eight: when more than 90% of Ruditapes philippinarum young shellfish is attached to the bottom of the breeding pond, performing intermediate breeding of indoor young shellfish, performing micro-inflation in the intermediate breeding process of the young shellfish, and culturing for 38 days by using the pond water as biological bait without changing water.
The method has the advantages that the monomer with the shell width and body length ratio arranged at the first 10% is selected as the parent in the seed selection process, the surface of the shell of the parent is not damaged, the single gram weight error between the parents is controlled to be smaller than 1.5g, the parent is subjected to intensive treatment and intensive culture, the quality of the parent can be effectively improved, the gonad development maturity of the parent is subjected to sampling inspection, spawning is induced according to the inspection result, the stability of artificial breeding is high, the quality of bred offspring is directly influenced by the quality of the parent, ruditapes philippinarum with stable monomer gram weight and body shape can be bred, the breeding yield can be improved by pretreating a breeding pond and carrying out ripening and spawning induction on the parent, the stress resistance of the artificially bred ruditapes philippinarum is high, and the economic benefit brought by artificial breeding can be improved.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (6)
1. An artificial breeding method for improving the yield of Ruditapes philippinarum is characterized by comprising the following steps:
the method comprises the following steps: selecting seeds, selecting a monomer with the ratio of the shell width to the body length arranged at the first 10% in a natural breeding or artificial breeding group as a parent, then carrying out strengthening treatment on the parent, specifically, disinfecting the parent by adopting a potassium permanganate aqueous solution, then putting the parent into a pre-culture pond for fine culture, specifically, feeding 0.5-1.0 mg/L of nutritive salt, soybean milk or shellfish compound feed, and hanging fermented and matured pig manure or chicken manure in the pre-culture pond with the dosage of 50 kg/mu of fertilizer water to ensure that the pond water is yellow brown;
step two: pretreating the seedling raising pool, namely, exposing the seedling raising pool to high temperature for sterilization, dredging and cleaning, and then constructing a seedling raising environment system in the seedling raising pool;
step three: ripening and stocking, screening the developmental maturity of the parents, directly stocking the parents with mature gonads to a nursery pond, ripening the parents with immature gonads firstly and then stocking, after ripening, randomly selecting 200 parents with 100 plus materials for sampling and dissection, and checking the gonad development degree;
step four: hastening parturition, when the gonad development degree inspection result in the third step shows that the parent gonad development degree is higher than 90 percent and the number of parent individuals exceeds 85 percent of the total number, hastening parturition on the parent in the seedling culture pond, discharging surface water in the seedling culture pond, and discharging redundant ruditapes philippinarum semen and excrement in the seedling culture pond;
step five: larva breeding, namely injecting a new water source into the seedling raising pool every day, ensuring that the water level in the seedling raising pool rises by 5-10cm every day, and ensuring that the transparency of water quality in the seedling raising pool is 40-50 cm;
step six: optimizing the larvae, namely optimizing Ruditapes philippinarum larvae in a nursery pond by using a 300-mesh silk net, and then culturing the Ruditapes philippinarum larvae according to the density of 2-3/mL;
step seven: when the body length of the Ruditapes philippinarum larvae reaches 200-;
step eight: when more than 90% of Ruditapes philippinarum young shellfish is attached to the bottom of the breeding pond, performing intermediate breeding of indoor young shellfish, performing micro-inflation in the intermediate breeding process of the young shellfish, and culturing for 30-45 days by using shrimp pond water as biological bait without changing water during the micro-inflation process to obtain Ruditapes philippinarum young shellfish seeds; in the seed selection process in the first step, the surface of the selected parent shell is ensured not to be damaged, and the single gram weight error between parents is controlled to be less than 1.5 g;
in the second step, the specific process of constructing the seedling environment system in the seedling pool is as follows: after the nursery pond is cleaned, laying the sterilized silt to the bottom of the nursery pond, wherein the laying thickness is 30-40cm, the silt is laid in 3-4 layers, the light salt water is sprayed once every layer of silt is laid, and then water is injected into the nursery pond, and the water depth is controlled to be 0.8-1.5 m;
in the second step, the specific processes of high-temperature insolation sterilization, desilting and cleaning are carried out on the nursery pond: the method comprises the steps of exposing a seedling raising pool at high temperature for 15-20 days until soil on the bottom of the pool is cracked, removing black and odorous sludge on the bottom of the pool, removing shells, stones and gravels on the bottom of the pool, turning over unoxidized pool soil on the bottom of the pool, fully oxidizing the mud, injecting water into the seedling raising pool to soak the pool until the bottom of the pool is flat, spraying bleaching water into the seedling raising pool to soak and sterilize for 2-3 days, discharging water in the seedling raising pool, and repeatedly washing with clean water for 2-3 times.
2. The artificial breeding method for improving the yield of Ruditapes philippinarum as claimed in claim 1, wherein the artificial breeding method comprises the following steps: in the third step, the process of ripening the parents with immature gonad development is as follows: ripening is accelerated by micro-aeration and artificial feeding of unicellular algae.
3. The artificial breeding method for improving the yield of Ruditapes philippinarum as claimed in claim 1, wherein the artificial breeding method comprises the following steps: in the fourth step, the specific method for hastening parturition is as follows: performing induced spawning by a method of combining drying in the shade for 0.5-3h and flowing water for 30-60min, and then performing air inflation incubation on the obtained mature Ruditapes philippinarum sperms and Ruditapes philippinarum ova, wherein the incubation density is 30-50/mL.
4. The artificial breeding method for improving the yield of Ruditapes philippinarum as claimed in claim 1, wherein the artificial breeding method comprises the following steps: in the fourth step, when the gonad development degree examination result in the third step shows that the number of parent individuals with parent gonad development degree higher than 90% is lower than 85% of the total number, the ripening treatment in the third step is continued until the parent gonad development process is higher than the spawning promotion requirement.
5. The artificial breeding method for improving the yield of Ruditapes philippinarum as claimed in claim 1, wherein the artificial breeding method comprises the following steps: and step five, when a new water source is injected into the seedling culture pond, the new water source needs to be filtered by a filter screen and injected slowly in the injection process.
6. The artificial breeding method for improving the yield of Ruditapes philippinarum as claimed in claim 1, wherein the artificial breeding method comprises the following steps: and step six, culturing the Ruditapes philippinarum larvae in a micro-aeration mode without changing water in a nursery pond during the culture period.
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| JP2016082967A (en) * | 2014-10-24 | 2016-05-19 | 学校法人東京農業大学 | Production method of hard-shell clam removed of acridity |
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103766246A (en) * | 2012-10-18 | 2014-05-07 | 嵊泗县海洋科技开发服务中心 | Artificial breeding method of Gomphina aequilatera |
| CN103766258A (en) * | 2014-01-26 | 2014-05-07 | 大连海洋大学 | Artificial breeding method of Ruditapes variegata |
| CN103828746A (en) * | 2014-03-04 | 2014-06-04 | 大连海洋大学 | Method for breeding ruditapes philippinarum |
| CN104855311A (en) * | 2015-05-08 | 2015-08-26 | 大连海洋大学 | Seed production method for new lines of wide-shell-type ruditapes philippinarum |
| CN105494193A (en) * | 2015-12-11 | 2016-04-20 | 福建省南日岛海洋生物技术有限公司 | Seedling culture method of Sanguinolaria diphos |
| CN110810289A (en) * | 2019-12-19 | 2020-02-21 | 大连宝发海珍品有限公司 | Method for breeding mottled clams |
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