CN104855311A - Seed production method for new lines of wide-shell-type ruditapes philippinarum - Google Patents
Seed production method for new lines of wide-shell-type ruditapes philippinarum Download PDFInfo
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- CN104855311A CN104855311A CN201510230047.1A CN201510230047A CN104855311A CN 104855311 A CN104855311 A CN 104855311A CN 201510230047 A CN201510230047 A CN 201510230047A CN 104855311 A CN104855311 A CN 104855311A
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- ruditapes philippinarum
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- 241000620877 Ruditapes philippinarum Species 0.000 title claims abstract description 43
- 238000004519 manufacturing process Methods 0.000 title abstract description 4
- 238000009395 breeding Methods 0.000 claims abstract description 18
- 230000001488 breeding effect Effects 0.000 claims abstract description 16
- 230000009027 insemination Effects 0.000 claims abstract description 5
- 230000032696 parturition Effects 0.000 claims abstract description 5
- 235000015170 shellfish Nutrition 0.000 claims description 24
- 238000000034 method Methods 0.000 claims description 22
- 230000013011 mating Effects 0.000 claims description 8
- 238000013461 design Methods 0.000 claims description 4
- 241000237852 Mollusca Species 0.000 description 8
- 230000000366 juvenile effect Effects 0.000 description 8
- 230000004083 survival effect Effects 0.000 description 5
- 230000001418 larval effect Effects 0.000 description 3
- 230000017448 oviposition Effects 0.000 description 3
- 230000008775 paternal effect Effects 0.000 description 3
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 230000007850 degeneration Effects 0.000 description 2
- 230000008774 maternal effect Effects 0.000 description 2
- 230000029052 metamorphosis Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000237519 Bivalvia Species 0.000 description 1
- 241001078425 Isochrysis zhanjiangensis Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241001115039 Ruditapes Species 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000012214 genetic breeding Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 210000002149 gonad Anatomy 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a seed production method for new lines of wide-shell-type ruditapes philippinarum. The seed production method comprises following steps: collecting multiple ruditapes philippinarum out of natural population, measuring width and length of a shell of each one of ruditapes philippinarum, arraying ruditapes philippinarum in the manner from large to small according to ratios among shell widths and shell lengths, selecting individuals ranking the top 10% thereof as the first generation of spats, utilizing the first generation of spats to set up multiple wide-shell-type families, utilizing a multi-trait Blup breeding method to select families with breeding values ranking the top 20% of families, selecting individuals with ratios among shell widths and shell lengths ranking the top 10% as the second generation of spats in accordance with the above mode in these families and breeding the second generation of spats, hastening parturition and carrying out artificial insemination and the like in order to obtain the line of wide-shell-type ruditapes philippinarum.
Description
Technical field
The present invention relates to a kind of producing method for seed of Ruditapes philippinarum, particularly a kind of shell wide type Ruditapes philippinarum new lines producing method for seed.
Background technology
Ruditapes philippinarum
(Ruditapes philippenarum)for wide temperature, eurysalinity kind, mainly be distributed in the Pacific Ocean and the Indian Ocean littoral, it is global cultivated shellfish, world's annual production about 3,780,000 t, account for Bivalvia aquaculture of aquatic animal output 23.5% (Ghiselli et al, 2012), wherein about 80% from China's Mainland, (Zhang Guofan & Yan likes force, 2010), wide market.But China's Ruditapes philippinarum output is unstable at present, and resistance is poor, and economic characters are degenerated year by year, constrains the development of Ruditapes philippinarum aquaculture industry.Cultivate high yield, degeneration-resistant Ruditapes philippinarum new varieties are the current in-problem main path of solution.
The shell mould of clam son is different, and shell morphology and growth, the economic characters such as degeneration-resistant are closely related, and this is that the research of genetic breeding provides abundant material.Research shows, shell wide type clam son has potential economic worth, its egg laying amount is large, fast growth, larva and juvenile mollusk survival rate high.Under identical housings elongate member, the young fresh weight of shell wide type clam and software weigh and are obviously greater than common clam son.In addition, shell wide type clam is young is young 2-3 times of common clam in Japanese market export price.Therefore, no matter be on biological significance, or in economics meaning, shell wide type Ruditapes philippinarum all has important Study and appliance and is worth.Although the wide type Ruditapes philippinarum of current shell can select acquisition in natural population, its output is lower and unstable, also cannot carry out artificial large-scale cultivation to shell wide shell mould Ruditapes philippinarum, can not meet the market demand far away.
Summary of the invention
The present invention is the above-mentioned deficiency in order to solve existing for prior art, based on quantitative inheritance theory, utilizes genetic and breeding method, proposes a kind of producing method for seed that can obtain shell wide type Ruditapes philippinarum strain.
Technical solution of the present invention is: a kind of shell wide type Ruditapes philippinarum new lines producing method for seed, it is characterized in that: described method is carried out according to following steps: in natural breed colony or artificial breeding colony, gather 1000-2000 grain Ruditapes philippinarum, and the shell measuring each Ruditapes philippinarum is respectively wide with shell long value, and calculate the wide ratio long with shell of its shell, all ratio is arranged according to mode from large to small, ratio is selected to come the individuality of front 10% as generation parent shellfish
Adopt the method for Nested Design by the generation parent shellfish selected according to 1 male clam is young and the ratio mating of 3 female clam sons, construct young family 30-60 of the wide type clam of shell, adopt the method for multiple characters Blup breeding, arrange according to breeding value mode from large to small in the young family of the wide type clam of the shell constructed, breeding value comes the family of front 20% as preferred family, and the shell measuring all individualities in each preferred family is respectively wide long with shell, calculate the wide ratio long with shell of its shell, all ratio is arranged according to mode from large to small, in each preferred family, select ratio to be arranged in front the individuality of 10%, individual mixing will be chosen as two generation parent shellfishes,
To the regular anatomic observation of two generation parent shellfishes, after discovery sperm enters active period, two generation parent shellfishes are moved on to indoor foster 3-5 days temporarily, afterwards two generation parent shellfishes are hastened parturition, and the female gamete of acquisition and male gamete are carried out artificial insemination in the mode of mass mating, obtain shell wide type Ruditapes philippinarum strain.
Compared with the existing technology, tool has the following advantages in the present invention:
The method disclosed in the present, the advantage there is ecological safety, importing without foreign gene, it can through simple operations, just the strain of the wide type Ruditapes philippinarum of shell of inheritance stability can be obtained efficiently, accurately, and the wide type Ruditapes philippinarum of shell that process this kind of method obtains is the new lines of Ruditapes philippinarum, its egg laying amount is large, fast growth, survival rate are high, and under identical housings elongate member, it is young that the young fresh weight of shell wide type clam is obviously greater than common clam.Because shell wide type clam young market export price is young 2-3 times of common clam, therefore this method has wide market prospects.
Accompanying drawing explanation
Fig. 1 is the long graph of a relation heavy with live body of the shell of the wide shell mould Ruditapes philippinarum of common Ruditapes philippinarum and shell.
Fig. 2 is the long graph of a relation heavy with software of the shell of the wide shell mould Ruditapes philippinarum of common Ruditapes philippinarum and shell.
The long-living long situation map of each family larval phase shell that Fig. 3 sets up for the embodiment of the present invention.
Each family larval phase absolute growth situation map that Fig. 4 sets up for the embodiment of the present invention.
The situation map of each family larval phase survival rate that Fig. 5 sets up for the embodiment of the present invention.
The long-living long situation map of each family juvenile mollusk phase shell that Fig. 6 sets up for the embodiment of the present invention.
Each family juvenile mollusk phase absolute growth situation map that Fig. 7 sets up for the embodiment of the present invention.
The situation map of each family juvenile mollusk phase survival rate that Fig. 8 sets up for the embodiment of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described.As shown in Figures 1 to 8: a kind of shell wide type Ruditapes philippinarum new lines producing method for seed, carry out according to following steps: in natural breed colony or artificial breeding colony, gather 1000-2000 grain Ruditapes philippinarum, and the shell measuring each Ruditapes philippinarum is respectively wide with shell long value, and calculate the wide ratio long with shell of its shell, all ratio is arranged according to mode from large to small, ratio is selected to come the individuality of front 10% as generation parent shellfish
Adopt the method for Nested Design by the generation parent shellfish selected according to 1 male clam is young and the ratio mating of 3 female clam sons, construct young family 30-60 of the wide type clam of shell, adopt the method for multiple characters Blup breeding, arrange according to breeding value mode from large to small in the young family of the wide type clam of the shell constructed, breeding value comes the family of front 20% as preferred family, and the shell measuring all individualities in each preferred family is respectively wide long with shell, calculate the wide ratio long with shell of its shell, all ratio is arranged according to mode from large to small, in each preferred family, select ratio to be arranged in front the individuality of 10%, individual mixing will be chosen as two generation parent shellfishes,
To the regular anatomic observation of two generation parent shellfishes, after discovery sperm enters active period, two generation parent shellfishes are moved on to indoor foster 3-5 days temporarily, afterwards two generation parent shellfishes are hastened parturition, and the female gamete of acquisition and male gamete are carried out artificial insemination in the mode of mass mating, obtain shell wide type Ruditapes philippinarum strain.
Embodiment
First, Ruditapes philippinarum wild population 2000 is gathered in From Zhangzi Islands China ecological preservation area, Dalian.Measure shell length and the shell width values of each Ruditapes philippinarum, and utilize Microsoft Excel software to calculate the wide ratio long with shell of shell, all ratio is arranged according to order from large to small, selects the individuality corresponding to ratio of front 10% wherein, as the generation parent shellfish of the wide type Ruditapes philippinarum of shell
Adopt the method for Nested Design by the generation parent shellfish selected according to 1 male clam is young and the ratio mating of 3 female clam sons, set up 11 paternal half-sib familys and 33 maternal family full-sibses, after the cultivation of 3 months, long, that shell the is wide heritability of the young paternal half-sib shell of shell wide type clam is respectively 0.76,0.35(is in table 1 to utilize SPSS software to estimate).Find that the wide type clam son of shell shows parent's shell mould feature gradually from the juvenile mollusk phase, utilize Blupf90 software estimation multiple characters Blup breeding value, find in set up family, front 20% is come with the breeding value being numbered B1, E1, F1, G2, K3, C3, J1 family, show the long and wide growth vigor of shell of significant shell
| Heritability | Shell is long | Shell is wide |
| Paternal half-sib | 0.763 | 0.350 |
| Maternal half sibs | 1.848 | 2.470 |
| Full sibs | 1.306 | 1.410 |
Table 1
Be numbered in the family of B1, E1, F1, G2, K3, C3, J1 above-mentioned, all Ruditapes philippinarums are carried out again the wide measurement with shell long value of shell, and utilize Microsoft Excel software to calculate the wide ratio long with shell of shell, all ratio is arranged according to order from large to small, select the individuality corresponding to ratio of front 10% wherein, as two generation parent shellfishes of the wide type Ruditapes philippinarum of shell
The two generation parent shellfishes selected are mixed, when its gonad maturity, regularly carry out anatomic observation, when indivedual sperm enlivens, two generation parent shellfishes are moved on to indoor continuation to support 3 ~ 5 days temporarily, the method of dry in the shade stimulation is adopted to hasten parturition afterwards, and adopt the method for mass mating, carry out artificial insemination, hatching, obtain shell wide type Ruditapes philippinarum strain.
Utilize conventional method, cultivate the larva of the shell wide type Ruditapes philippinarum strain obtained and juvenile mollusk, nurturing period continuous micro inflation, every day, full dose changed water 1 time, day bait throwing in 3-4 time, and bait is lsochrysis zhanjiangensis
(Isochrysis zhanjiangensis), daily ration, feeding quantity is depending on the situation of ingesting.15 ~ 20 days are needed to completing metamorphosis (pointing out the raw shell of occurrence) from larva dive.After metamorphosis, through the cultivation of 20 ~ 30 days, when juvenile mollusk shell reaches more than 700 μm, forward outdoor earth pond to and carry out Transitional culture.The egg laying amount of the young strain of the wide type clam of shell and just to incubate D type embryophore long in table 1, the long-living length of the young strain embryophore of shell wide type clam is in table 2, and the young strain long-living length of juvenile mollusk shell of shell wide type clam and survival are in table 3.Cultivate after 120 days, the young strain of the wide type clam of shell obtains the filial generation 4.5 ten thousand pieces of the long 0.5 ~ 1.1cm of shell, and the shell of filial generation is wide is all greater than common natural population clam son, and the young filial performance of the wide type clam of shell goes out parent's shell mould feature.
Claims (1)
1. a shell wide type Ruditapes philippinarum new lines producing method for seed, it is characterized in that: described method is carried out according to following steps: in natural breed colony or artificial breeding colony, gather 1000-2000 grain Ruditapes philippinarum, and the shell measuring each Ruditapes philippinarum is respectively wide with shell long value, and calculate the wide ratio long with shell of its shell, all ratio is arranged according to mode from large to small, ratio is selected to come the individuality of front 10% as generation parent shellfish
Adopt the method for Nested Design by the generation parent shellfish selected according to 1 male clam is young and the ratio mating of 3 female clam sons, construct young family 30-60 of the wide type clam of shell, adopt the method for multiple characters Blup breeding, arrange according to breeding value mode from large to small in the young family of the wide type clam of the shell constructed, breeding value comes the family of front 20% as preferred family, and the shell measuring all individualities in each preferred family is respectively wide long with shell, calculate the wide ratio long with shell of its shell, all ratio is arranged according to mode from large to small, in each preferred family, select ratio to be arranged in front the individuality of 10%, individual mixing will be chosen as two generation parent shellfishes,
To the regular anatomic observation of two generation parent shellfishes, after discovery sperm enters active period, two generation parent shellfishes are moved on to indoor foster 3-5 days temporarily, afterwards two generation parent shellfishes are hastened parturition, and the female gamete of acquisition and male gamete are carried out artificial insemination in the mode of mass mating, obtain shell wide type Ruditapes philippinarum strain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510230047.1A CN104855311B (en) | 2015-05-08 | 2015-05-08 | The wide type Ruditapes philippinarum strain producing method for seed of shell |
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| CN201510230047.1A CN104855311B (en) | 2015-05-08 | 2015-05-08 | The wide type Ruditapes philippinarum strain producing method for seed of shell |
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| CN104855311B CN104855311B (en) | 2018-05-29 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106755441A (en) * | 2016-12-29 | 2017-05-31 | 华南农业大学 | A kind of method that gene group selection based on multiple characters carries out forest multiple characters pyramiding breeding |
| CN107926790A (en) * | 2017-12-08 | 2018-04-20 | 重庆金宇恒科技有限公司 | A kind of producing method for seed for the Perna viridis strain that encrusts |
| CN111183937A (en) * | 2020-03-04 | 2020-05-22 | 大连海洋大学 | Artificial breeding method for improving yield of Ruditapes philippinarum |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101288387A (en) * | 2008-06-11 | 2008-10-22 | 大连水产学院 | Seed production method of Philippines little clam breeding lines |
| CN102388828A (en) * | 2011-10-09 | 2012-03-28 | 中国科学院南海海洋研究所 | Breeding method for rapid growing line of seawater pearl shells |
| CN103798165A (en) * | 2014-01-23 | 2014-05-21 | 中国科学院南海海洋研究所 | Good nacre cultivating method for pinctada martensii |
-
2015
- 2015-05-08 CN CN201510230047.1A patent/CN104855311B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101288387A (en) * | 2008-06-11 | 2008-10-22 | 大连水产学院 | Seed production method of Philippines little clam breeding lines |
| CN102388828A (en) * | 2011-10-09 | 2012-03-28 | 中国科学院南海海洋研究所 | Breeding method for rapid growing line of seawater pearl shells |
| CN103798165A (en) * | 2014-01-23 | 2014-05-21 | 中国科学院南海海洋研究所 | Good nacre cultivating method for pinctada martensii |
Non-Patent Citations (1)
| Title |
|---|
| 霍忠明等: "菲律宾蛤仔(Ruditapes philippinarum)大连群体家系建立及生长比较*", 《海 洋 与 湖 沼》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106755441A (en) * | 2016-12-29 | 2017-05-31 | 华南农业大学 | A kind of method that gene group selection based on multiple characters carries out forest multiple characters pyramiding breeding |
| CN106755441B (en) * | 2016-12-29 | 2020-08-07 | 华南农业大学 | Method for performing forest multi-character polymerization breeding based on multi-character genome selection |
| CN107926790A (en) * | 2017-12-08 | 2018-04-20 | 重庆金宇恒科技有限公司 | A kind of producing method for seed for the Perna viridis strain that encrusts |
| CN111183937A (en) * | 2020-03-04 | 2020-05-22 | 大连海洋大学 | Artificial breeding method for improving yield of Ruditapes philippinarum |
| CN111183937B (en) * | 2020-03-04 | 2022-02-01 | 大连海洋大学 | Artificial breeding method for improving yield of Ruditapes philippinarum |
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| CN104855311B (en) | 2018-05-29 |
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