CN104855311B - The wide type Ruditapes philippinarum strain producing method for seed of shell - Google Patents
The wide type Ruditapes philippinarum strain producing method for seed of shell Download PDFInfo
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- CN104855311B CN104855311B CN201510230047.1A CN201510230047A CN104855311B CN 104855311 B CN104855311 B CN 104855311B CN 201510230047 A CN201510230047 A CN 201510230047A CN 104855311 B CN104855311 B CN 104855311B
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- ruditapes philippinarum
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- 241000620877 Ruditapes philippinarum Species 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 23
- 235000015170 shellfish Nutrition 0.000 claims abstract description 27
- 238000009395 breeding Methods 0.000 claims abstract description 16
- 230000032696 parturition Effects 0.000 claims abstract description 5
- 230000001488 breeding effect Effects 0.000 claims description 14
- 230000013011 mating Effects 0.000 claims description 7
- 238000005259 measurement Methods 0.000 claims description 5
- 238000013461 design Methods 0.000 claims description 4
- 230000009027 insemination Effects 0.000 abstract description 4
- 241000237852 Mollusca Species 0.000 description 7
- 230000000366 juvenile effect Effects 0.000 description 7
- 230000004083 survival effect Effects 0.000 description 4
- 230000002068 genetic effect Effects 0.000 description 3
- 230000001418 larval effect Effects 0.000 description 3
- 230000008775 paternal effect Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 230000007850 degeneration Effects 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 230000008774 maternal effect Effects 0.000 description 2
- 230000017448 oviposition Effects 0.000 description 2
- 241000237519 Bivalvia Species 0.000 description 1
- 241001078425 Isochrysis zhanjiangensis Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241001115039 Ruditapes Species 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000012214 genetic breeding Methods 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 230000029052 metamorphosis Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention discloses a kind of wide type Ruditapes philippinarum strain producing method for seed of shell：If dry granular Ruditapes philippinarum is gathered in natural population, and the shell that measures each Ruditapes philippinarum respectively is wide with shell long value, the wide ratio with shell length of its shell is arranged in the way of from large to small, and the individual for coming preceding 10% is chosen as generation parent shellfish, using generation parent shellfish build wide shell mould family several, preceding 20% family is come using multiple characters Blup breeding method selection and use values, and in these familys again according to aforesaid way choose the wide length with shell of shell than preceding 10% individual as two generation parent shellfishes, and two generation parent shellfishes are cultivated, it hastens parturition, the modes such as artificial insemination are handled, obtain the wide type Ruditapes philippinarum strain of shell.
Description
Technical field
The present invention relates to a kind of producing method for seed of Ruditapes philippinarum, particularly a kind of wide type Ruditapes philippinarum strain production of hybrid seeds of shell
Method.
Background technology
Ruditapes philippinarum(Ruditapes philippenarum)For wide temperature, eurysalinity species, the Pacific Ocean is distributed mainly on
And the Indian Ocean is littoral, is global cultivated shellfish, annual output about 3,780,000 t in the world's accounts for Bivalvia aquaculture of aquatic animal yield
23.5% (Ghiselli et al, 2012), wherein about 80% from China's Mainland (Zhang Guofan Yans like military, 2010), market prospects
It is wide.But current China Ruditapes philippinarum yield is unstable, and resistance is poor, and economic characters are degenerated year by year, constrains philippine clam
The development of young aquaculture industry.Cultivate high yield, degeneration-resistant Ruditapes philippinarum new varieties be solve the problems, such as presently, there are main path.
The shell mould of clam son is different, and shell morphology and the economic characters such as growth, degeneration-resistant are closely related, this is the research of genetic breeding
Provide abundant material.Research, which shows that the wide type clam of shell is young, has potential economic value, egg laying amount is big, the speed of growth is fast,
Larva and juvenile mollusk survival rate are high.Under identical housings elongate member, the wide type clam son fresh weight of shell and software are significantly greater than common clam son again.Separately
Outside, the wide type clam son of shell is common 2-3 times of clam son in Japanese market export price.Therefore, whether on biological significance, still
In economics meaning, the wide type Ruditapes philippinarum of shell all has important research and application value.The wide type Ruditapes philippinarum of shell at present
Although acquisition can be selected in natural population, its yield is relatively low and unstable, can not also be to the wide shell mould Ruditapes philippinarum of shell
Artificial large-scale cultivation is carried out, much can not meet the market demand.
The content of the invention
The present invention is to solve the above-mentioned deficiency present in the prior art, based on quantitative inheritance theory, is utilized
Genetic and breeding method proposes a kind of producing method for seed that can obtain the wide type Ruditapes philippinarum strain of shell.
The present invention technical solution be：A kind of wide type Ruditapes philippinarum strain producing method for seed of shell, it is characterised in that：Institute
The method stated follows the steps below：1000-2000 Philippine are gathered in natural breed group or artificial breeding group
Clam is young, and the shell for measuring each Ruditapes philippinarum respectively is wide with shell long value, and calculates the wide ratio with shell length of its shell, will be all
Ratio arranges in the way of from large to small, and ratio is selected to come preceding 10% individual as generation parent shellfish,
Using the method for Nested Design by the generation parent shellfish selected according to 1 young ratio with 3 female clam sons of male clam
Mating constructs wide type clam son family 30-60, shell, using the method for multiple characters Blup breedings, in the wide type clam of shell constructed
In young family by breeding value from large to small in the way of arrange, breeding value comes preceding 20% family as preferred family, and respectively
The shell of all individuals is wide long with shell in each preferably family of measurement, calculates the wide ratio with shell length of its shell, all ratio is pressed
It is arranged according to mode from large to small, in each preferred family ratio is selected to be arranged in front 10% individual, individual mixing will be chosen
As two generation parent shellfishes,
Two generation parent shellfishes are periodically dissected and observed, after finding that sperm enters active period, two generation parent shellfishes are moved on to indoor temporarily foster
It 3-5 days, hastens parturition afterwards to two generation parent shellfishes, and the female gamete of acquisition and male gamete is carried out in a manner of mass mating
Artificial insemination obtains the wide type Ruditapes philippinarum strain of shell.
The present invention compared with the existing technology, has the following advantages that：
The method disclosed in the present has the advantages that ecological safety, is imported without foreign gene, it can pass through simple behaviour
Make, can efficiently, accurately obtain the strain of the wide type Ruditapes philippinarum of shell of inheritance stability, and obtained by this kind of method
The wide type Ruditapes philippinarum of shell is the new lines of Ruditapes philippinarum, and egg laying amount is big, the speed of growth is fast, survival rate is high, in identical housings
Under elongate member, it is young that the wide type clam son's fresh weight of shell is significantly greater than common clam.Since the wide type clam son market export price of shell is that common clam is young
2-3 times, therefore this method has a vast market prospect.
Description of the drawings
Fig. 1 is common Ruditapes philippinarum and the shell length of the wide shell mould Ruditapes philippinarum of shell and the relational graph of live body weight.
Fig. 2 is common Ruditapes philippinarum and the shell length of the wide shell mould Ruditapes philippinarum of shell and the relational graph of software weight.
Each family larval phase shell length growth situation map that Fig. 3 is established by the embodiment of the present invention.
Each family larval phase absolute growth situation map that Fig. 4 is established by the embodiment of the present invention.
The situation map for each family larval phase survival rate that Fig. 5 is established by the embodiment of the present invention.
Each family juvenile mollusk phase shell length growth situation map that Fig. 6 is established by the embodiment of the present invention.
Each family juvenile mollusk phase absolute growth situation map that Fig. 7 is established by the embodiment of the present invention.
The situation map for each family juvenile mollusk phase survival rate that Fig. 8 is established by the embodiment of the present invention.
Specific embodiment
Below in conjunction with the specific embodiment of the description of the drawings present invention.As shown in Figures 1 to 8：A kind of luxuriant and rich with fragrance rule of the wide type of shell
Guest's clam son's strain producing method for seed, follows the steps below：1000- is gathered in natural breed group or artificial breeding group
2000 Ruditapes philippinarums, and the shell that measures each Ruditapes philippinarum respectively is wide with shell long value, and it is wide with shell length to calculate its shell
Ratio arranges all ratio in the way of from large to small, and ratio is selected to come preceding 10% individual as generation parent shellfish,
Using the method for Nested Design by the generation parent shellfish selected according to 1 young ratio with 3 female clam sons of male clam
Mating constructs wide type clam son family 30-60, shell, using the method for multiple characters Blup breedings, in the wide type clam of shell constructed
In young family by breeding value from large to small in the way of arrange, breeding value comes preceding 20% family as preferred family, and respectively
The shell of all individuals is wide long with shell in each preferably family of measurement, calculates the wide ratio with shell length of its shell, all ratio is pressed
It is arranged according to mode from large to small, in each preferred family ratio is selected to be arranged in front 10% individual, individual mixing will be chosen
As two generation parent shellfishes,
Two generation parent shellfishes are periodically dissected and observed, after finding that sperm enters active period, two generation parent shellfishes are moved on to indoor temporarily foster
It 3-5 days, hastens parturition afterwards to two generation parent shellfishes, and the female gamete of acquisition and male gamete is carried out in a manner of mass mating
Artificial insemination obtains the wide type Ruditapes philippinarum strain of shell.
Embodiment
First, Ruditapes philippinarum wild population 2000 is gathered in Dalian Zhangzi Islands China ecological preservation area.The each luxuriant and rich with fragrance rule of measurement
The shell of guest clam son is long and shell width values, and calculates the wide ratio with shell length of shell using Microsoft Excel software, by all ratio according to
Order from large to small is arranged, the individual before selecting wherein corresponding to 10% ratio, as the wide type Ruditapes philippinarum of shell
Generation parent shellfish,
Using the method for Nested Design by the generation parent shellfish selected according to 1 young ratio with 3 female clam sons of male clam
Mating, establishes 11 paternal half-sib familys and 33 maternal family full-sibses, soft using SPSS after the cultivation of 3 months
The genetic force that the part estimation wide type clam son paternal half-sib shell of shell is long, shell is wide is respectively 0.76,0.35（It is shown in Table 1）.It was found that the wide type of shell
Clam son gradually shows parent's shell mould feature since the juvenile mollusk phase, utilizes Blupf90 software estimation multiple characters Blup breeding values, hair
In the family established now, preceding 20% is come with the breeding value that number is B1, E1, F1, G2, K3, C3, J1 family, shows to show
The shell of work is long and the wide growth vigor of shell,
| Genetic force | Shell is long | Shell is wide |
| Paternal half-sib | 0.763 | 0.350 |
| Maternal half sibs | 1.848 | 2.470 |
| Full sibs | 1.306 | 1.410 |
Table 1
In above-mentioned number is the family of B1, E1, F1, G2, K3, C3, J1, all Ruditapes philippinarums are carried out again
The wide measurement with shell long value of shell, and the wide ratio with shell length of shell is calculated using Microsoft Excel software, by all ratio according to by
Big extremely small order is arranged, the individual before selecting wherein corresponding to 10% ratio, as the wide type Ruditapes philippinarum of shell
Two generation parent shellfishes,
The two generation parent shellfishes selected are mixed, when its sexual gland maturation, are periodically dissected and observed, when indivedual sperms are lived
Two generation parent shellfishes are moved on to indoor continuation during jump temporarily to support 3~5 days, are hastened parturition afterwards using the method for dry in the shade stimulation, and using group
The method of body mating carries out artificial insemination, hatching, obtains the wide type Ruditapes philippinarum strain of shell.
Using conventional method, the larva and juvenile mollusk of the wide type Ruditapes philippinarum strain of the shell of acquisition are cultivated, cultivated
Period continuous micro is inflated, and daily full dose changes water 1 time, and day bait throwing in 3-4 times, bait is lsochrysis zhanjiangensis(Isochrysis zhanjiangensis), daily ration, feeding quantity is depending on the situation of ingesting.It is abnormal from larva dive to completing（Point out that occurrence gives birth to shell）Need 15~
20 days.After metamorphosis, through the cultivation of 20~30 days, when juvenile mollusk shell is up to 700 μm or more, goes to outdoor earth pond and carry out intermediate educate
Into.After cultivating 120 days, the wide type clam son strain of shell obtains 4.5 ten thousand pieces of the filial generation that shell grows 0.5 ~ 1.1cm, and the shell of filial generation is wide to be all higher than
Common natural population clam is young, and the wide type clam son's filial performance of shell goes out parent's shell mould feature.
Claims (1)
1. a kind of wide type Ruditapes philippinarum strain producing method for seed of shell, it is characterised in that：The method follows the steps below：
1000-2000 Ruditapes philippinarums are gathered in natural breed group or artificial breeding group, and measure each Philippine respectively
The shell of clam son is wide with shell long value, and calculates the wide ratio with shell length of its shell, and all ratio is arranged in the way of from large to small
Row select ratio to come preceding 10% individual as generation parent shellfish,
The generation parent shellfish selected is mated according to 1 young ratio with 3 female clam sons of male clam using the method for Nested Design,
Wide type clam son family 30-60, shell is constructed, using the method for multiple characters Blup breedings, in the wide type clam son's family of shell constructed
In by breeding value from large to small in the way of arrange, breeding value comes preceding 20% family as preferred family, and measurement is every respectively
The shell of all individuals is wide long with shell in a preferred family, the wide ratio with shell length of its shell is calculated, by all ratio according to by big
It is arranged to small mode, ratio is selected to be arranged in front 10% individual in each preferred family, individual mixing will be chosen as two
Dai Qinbei,
Two generation parent shellfishes are periodically dissected and observed, after finding that sperm enters active period, two generation parent shellfishes are moved on into indoor temporarily foster 3-5
My god, hasten parturition afterwards to two generation parent shellfishes, and by the female gamete of acquisition and male gamete in a manner of mass mating into pedestrian
Work is inseminated, and obtains the wide type Ruditapes philippinarum strain of shell.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510230047.1A CN104855311B (en) | 2015-05-08 | 2015-05-08 | The wide type Ruditapes philippinarum strain producing method for seed of shell |
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|---|---|---|---|
| CN201510230047.1A CN104855311B (en) | 2015-05-08 | 2015-05-08 | The wide type Ruditapes philippinarum strain producing method for seed of shell |
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| CN104855311B true CN104855311B (en) | 2018-05-29 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106755441B (en) * | 2016-12-29 | 2020-08-07 | 华南农业大学 | Method for performing forest multi-character polymerization breeding based on multi-character genome selection |
| CN107926790A (en) * | 2017-12-08 | 2018-04-20 | 重庆金宇恒科技有限公司 | A kind of producing method for seed for the Perna viridis strain that encrusts |
| CN111183937B (en) * | 2020-03-04 | 2022-02-01 | 大连海洋大学 | Artificial breeding method for improving yield of Ruditapes philippinarum |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101288387A (en) * | 2008-06-11 | 2008-10-22 | 大连水产学院 | Seed production method of Philippines little clam breeding lines |
| CN102388828A (en) * | 2011-10-09 | 2012-03-28 | 中国科学院南海海洋研究所 | Breeding method for rapid growing line of seawater pearl shells |
| CN103798165B (en) * | 2014-01-23 | 2016-02-10 | 中国科学院南海海洋研究所 | A kind of breeding method of pteria martensii excellent pinctada |
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