CN113678768A - Breeding method of fast-growing stress-resistant Chinese prawn complete set line - Google Patents
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- 241000238557 Decapoda Species 0.000 title claims abstract description 190
- 238000009395 breeding Methods 0.000 title claims abstract description 54
- 230000001488 breeding effect Effects 0.000 claims abstract description 47
- 230000035882 stress Effects 0.000 claims abstract description 41
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims abstract description 18
- 230000009027 insemination Effects 0.000 claims abstract description 16
- 230000008774 maternal effect Effects 0.000 claims abstract description 16
- 230000013011 mating Effects 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 12
- 230000008650 pH stress Effects 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 238000009360 aquaculture Methods 0.000 claims description 9
- 244000144974 aquaculture Species 0.000 claims description 9
- 230000037396 body weight Effects 0.000 claims description 8
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 6
- 210000001672 ovary Anatomy 0.000 claims description 6
- 230000008775 paternal effect Effects 0.000 claims description 6
- KEQXNNJHMWSZHK-UHFFFAOYSA-L 1,3,2,4$l^{2}-dioxathiaplumbetane 2,2-dioxide Chemical compound [Pb+2].[O-]S([O-])(=O)=O KEQXNNJHMWSZHK-UHFFFAOYSA-L 0.000 claims description 4
- 235000019270 ammonium chloride Nutrition 0.000 claims description 3
- 230000004083 survival effect Effects 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 241000238553 Litopenaeus vannamei Species 0.000 description 4
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 238000009396 hybridization Methods 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 241001124325 Marsupenaeus japonicus Species 0.000 description 2
- 239000004698 Polyethylene Substances 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000012447 hatching Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- -1 polyethylene Polymers 0.000 description 2
- 229920000573 polyethylene Polymers 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 231100000570 acute poisoning Toxicity 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 231100000739 chronic poisoning Toxicity 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000006353 environmental stress Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000009405 line breeding Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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Abstract
The invention discloses a breeding method of a fast-growing stress-resistant Chinese prawn mating line, belonging to the technical field of Chinese prawn breeding, wherein the method comprises the steps of collecting the 'yellow sea No. 1' population of Chinese prawn for pure propagation, selecting excellent-growth female prawn and male prawn parent from the 'yellow sea No. 1' pure propagation population of Chinese prawn as a ancestor, and breeding a family line with high growth performance as a specialized maternal line; collecting the 'yellow sea No. 3' and 'yellow sea No. 4' populations of Chinese prawns for pure breeding, selecting male shrimp parents with strong high ammonia nitrogen stress resistance from the 'yellow sea No. 3' pure breeding populations of the Chinese prawns, selecting female shrimp parents with strong high pH stress resistance from the 'yellow sea No. 4' pure breeding populations of the Chinese prawns as ancestors, and adopting an artificial insemination technology to breed families with strong stress resistance as specialized father lines; selecting female shrimps from a specialized female line, selecting male shrimps from a specialized father line, and hybridizing by adopting an artificial insemination technology to obtain the fast-growing stress-resistant Chinese prawn complete set line.
Description
Technical Field
The invention belongs to the technical field of Chinese prawn breeding, and particularly relates to a breeding method of a fast-growing stress-resistant Chinese prawn complete set line.
Background
The Chinese prawn is also known as oriental prawn, commonly known as prawn and prawn, is an important culture species in coastal areas of yellow Bohai sea in China, and has a culture yield of about 20 million tons in the peak period of culture development (before and after 1990), which accounts for 70 percent of the culture yield of Chinese prawn. However, since the outbreak of viral diseases of prawns in 1993, the total culture yield of Chinese prawns is sharply reduced, the production is gradually recovered until 2000 years later, and in recent years, the culture area is 30-40 ten thousand mu, and the culture yield is 4-5 ten thousand tons. In recent years, the growing environment of Chinese prawns is seriously influenced by pollution of aquaculture water environment and frequent extreme weather, and the growth and development of Chinese prawns are inhibited by the stress of abnormal aquaculture environment factors, so that the yield of the Chinese prawns is reduced and even the Chinese prawns die.
The national fishery water quality standard (GB11607-89) of China requires that the concentration of non-ionic ammonia in water in a fishery water area is less than or equal to 0.02mg/L, but in the actual pond culture process, due to the reasons of high stocking density, excessive bait feeding, years of accumulation of excrement and the like, the content of ammonia nitrogen in a pond is high frequently, so that the immunity and the resistance of prawns are reduced, the prawns grow slowly, diseases occur, even die due to acute and chronic poisoning, and great harm is brought to the aquaculture. The pH value is used as the index of the ecological balance of the water environment, and the concentration of the nonionic ammonia in the water body is increased by 10 times when one pH value unit is increased. The survival rate of the young Chinese prawn is over 95 percent in 24 hours under the condition of normal pH value, the survival rate is obviously reduced along with the increase of the pH value, the death rate of the young Chinese prawn can reach 85 percent in 6 hours under the condition of pH9.4, and all the young Chinese prawn dies in 24 hours. Compared with other main breeding species such as litopenaeus vannamei and marsupenaeus japonicus, the tolerance of the litopenaeus vannamei under high pH stress is lowest, the survival rates of the litopenaeus vannamei and the marsupenaeus japonicus are respectively 54.6 percent and 97 percent under the same high pH stress condition, and the survival rate of the litopenaeus vannamei is only 13.3 percent. Therefore, the discovery of Chinese prawn germplasm materials with excellent stress resistance and the cultivation of new Chinese prawn varieties with high growth speed and strong stress resistance to adapt to and resist various stresses and adverse stimuli caused by culture environmental factors is one of the primary tasks of the current prawn breeding work.
At present, the breeding of Chinese prawn is mainly group breeding and family breeding, the breeding target is mostly single character breeding, and the requirement of the current breeding industry for diversified new species is difficult to meet. The matched line breeding is to select special Chinese prawn lines with different excellent characters, and to screen out commodity generations with excellent hybrid advantages and meeting production requirement through multiple matching and hybridization. Therefore, the breeding work of the mating line of the Chinese prawns needs to be developed urgently, new germplasm with high growth speed and strong stress resistance is cultivated, the breeding success rate and the improved variety coverage rate of the Chinese prawns are improved, and the stable and sustainable development of the industry is promoted.
Disclosure of Invention
The invention aims to solve the technical problem of providing a breeding method of a fast-growing stress-resistant Chinese prawn complete set line, which fully utilizes the genetic resource advantages of new varieties of cultured Chinese prawns 'yellow sea No. 1', 'yellow sea No. 3' and 'yellow sea No. 4', fully exerts the advantages of hybridization among the varieties on the basis of keeping the excellent growth and stress resistance of the varieties, polymerizes the excellent characters and breeds the Chinese prawn complete set line with high growth speed and strong tolerance capacity to environmental stress.
The invention is realized by the following technical scheme:
a breeding method of a fast-growing stress-resistant Chinese prawn complete set line comprises the following operation steps:
(1) collecting the 'yellow sea No. 1' group of Chinese prawn for pure reproduction, selecting individuals with excellent growth performance to construct a basic group, and using the basic group as a progenitor source of a mating line maternal line;
(2) collecting the 'yellow sea No. 3' and 'yellow sea No. 4' groups of the Chinese prawn for pure reproduction, respectively selecting individuals with strong high ammonia nitrogen and high pH stress tolerance capability in the aquaculture water body from the two groups to construct a basic group, and using the basic group as an ancestral source of a parent line of a mating line;
(3) selecting excellent female shrimp and male shrimp parents as ancestors from the pure breeding group of Chinese prawn 'yellow sea No. 1', hybridizing by adopting an artificial insemination technology, and breeding a family with high growth performance as a specialized maternal line;
(4) selecting male shrimp parents with strong high ammonia nitrogen stress resistance from the 'yellow sea No. 3' pure breeding population of Chinese prawns, selecting female shrimp parents with strong high pH stress resistance from the 'yellow sea No. 4' pure breeding population of Chinese prawns as ancestors, hybridizing by adopting an artificial insemination technology, and breeding a family with strong stress resistance as a specialized father line;
(5) selecting female shrimps from a specialized female line, selecting male shrimps from a specialized father line, and hybridizing by adopting an artificial insemination technology to obtain the fast-growing stress-resistant Chinese prawn complete set line.
The further technical scheme is as follows:
the group of the Chinese prawn, yellow sea No. 1, in the step (1) has the excellent character of high growth speed, and the registration number of the breed is GS 01001-2003;
the group of the Chinese prawn, yellow sea No. 3, in the step (2) has strong ammonia nitrogen stress resistance, and the variety registration number is GS-01-002-; the Chinese prawn, yellow sea No. 4, has strong high pH value stress resistance, and the variety registration number is GS-01-003-.
The selection criteria of female shrimp and male shrimp parents in the step (3) are as follows: the body constitution is strong, the appendages are complete, the body surface is smooth, the ovaries of the female shrimps grow well, the body length is more than 14cm, and the body weight is more than 37 g; the male shrimp is milk white, and has a length of more than 12cm and a weight of more than 24 g.
The method for cultivating the specialized maternal line in the step (3) comprises the following operation steps:
s1, selecting female shrimp and male shrimp parents with excellent growth characters, and hybridizing by adopting an artificial insemination technology according to a ratio of 1:1 to produce F1 generations;
s2, breeding 3-5 families with high growth speed from the F1 generation, and performing in-family selfing from the F2 generation;
s3, finally, selecting the family with the best growth character performance as a specialized maternal line through continuous breeding of 3 generations.
In the step S3, the parent selection standard of the specialized maternal line is as follows: the body constitution is strong, the appendages are complete, the body surface is smooth, the ovaries of the female shrimps grow well, the body length is more than 16cm, and the body weight is more than 47 g; the male shrimp is milk white, and has a length of more than 13cm and a weight of more than 31 g.
The selection standard of the male shrimp parent of the Chinese prawn 'yellow sea No. 3' in the step (4) is as follows: the ammonium chloride concentration is 64mg/L, the shrimp survives under the condition of stress for 96 hours, the length of the male shrimp is more than 12cm, and the weight of the male shrimp is more than 21 g; the selection standard of female Chinese prawn "yellow sea No. 4" parent is as follows: the individuals surviving under the condition of stress of pH9.2 for 96h have the length of the un-copulated female shrimp larger than 14cm and the weight larger than 33 g.
The cultivation method of the specialized father line in the step (4) comprises the following operation steps:
s1, selecting male shrimps of the Chinese prawn 'yellow sea No. 3' and female shrimps of the Chinese prawn 'yellow sea No. 4', hybridizing by adopting an artificial insemination technology to produce F1 generations;
s2, breeding 3-5 families with strong high ammonia nitrogen resistance and high pH value stress resistance from the F1 generation, and performing in-family selfing from the F2 generation;
s3, finally, breeding the family with the strongest stress resistance as a specialized father line through continuous breeding of 3 generations.
In the step S3, the selection criteria of the specialized paternal parent are: compared with the parent generation, the high ammonia nitrogen resistance and high pH resistance of the young shrimps are improved by more than 30%, the ovaries of the female shrimps are full, the body length is more than 14cm, the weight is more than 36g, and the spermatophore of the male shrimps is milky white, the body length is more than 13cm, and the weight is more than 27 g.
And (5) selecting female shrimps from the specialized maternal line, selecting male shrimps from the specialized paternal line, and hybridizing to obtain the matched line high-quality commercial shrimps, wherein the commercial shrimps are strong in physique, complete in appendages, smooth and clean in body surface, and improved in growth performance and stress resistance compared with the parent shrimps. The high ammonia nitrogen resistance and high pH value resistance of the young shrimps is improved by more than 20 percent compared with the ancestors, the length of the harvested female shrimps is more than 15cm, the weight of the harvested female shrimps is more than 43g, the length of the harvested male shrimps is more than 13cm, and the weight of the harvested male shrimps is more than 29 g.
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FIG. 1 is a technical flow chart of the breeding of fast-growing stress-resistant Chinese prawn complete set line of the present invention.
Detailed Description
The present invention will be described in detail with reference to the following embodiments in order to make the features and advantages of the invention more comprehensible. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, but rather should be construed as broadly as the present invention is capable of modification in various respects, all without departing from the spirit and scope of the present invention.
Example 1: collection of basic population of Chinese prawn mating line ancestor
The Chinese prawn 'yellow sea No. 1', 'yellow sea No. 3' and 'yellow sea No. 4' are all the new aquatic product varieties approved by the nation, the variety registration numbers are respectively GS01001-2003, GS-01-002 ion-doped 2013 and GS-01-003 ion-doped 2020, and the Chinese prawn 'yellow sea No. 1' has the excellent character of high growth speed, the Chinese prawn 'yellow sea No. 3' has strong tolerance to ammonia nitrogen stress in the aquaculture water body, and the Chinese prawn 'yellow sea No. 4' has strong tolerance to high pH value in the aquaculture water body. The implementation place is set in Haifeng aquaculture Limited liability company in Changyi city, the used parent materials are purchased from the Haifeng aquaculture Limited liability company in Changyi city, the culture environment and the management conditions are basically consistent, errors caused by artificial and environmental factors on breeding can be eliminated, and the accuracy of the breeding effect is improved. The growth, ammonia nitrogen resistance and high pH stress resistance of 3 populations were tested in a full double-row hybridization mode, and the results are shown in the following table.
Example 2: performance selection standard of Chinese prawn mating line mother line and ancestor
When the breeding group of the Chinese prawn 'yellow sea No. 1' is harvested 10 months in 2015, 100 tails of non-copulated female prawns and male prawns with strong physique and good growth performance are selected from the breeding group of the Chinese prawn 'yellow sea No. 1' to be individually reserved and overwintering, and the body length and the weight of the non-copulated female prawns and the male prawns are counted to be used as the ancestor of the mating line maternal line. The collected Chinese prawn, yellow sea No. 1, has a length of 14.65-15.35cm and a weight of 37.01-42.37 g; the male shrimp has a length of 12.7-13.62cm and a weight of 24.02-27.53 g.
Example 3: performance selection standard of Chinese prawn mating line parent line and ancestor
And (3) carrying out offspring seed breeding on the collected parent shrimps of the basic groups of the Chinese shrimps of the yellow sea No. 3 and the yellow sea No. 4 in the year of 2015 for 4, and carrying out a stress experiment when the larvae grow to the larvae with the body length of about 1.0 cm.
(1) Randomly selecting 10 million young shrimps from the yellow sea No. 3 population of the Chinese shrimps, and putting about 2.5 million survived individuals into an outdoor pond for breeding after the ammonium chloride with the concentration of 64mg/L is used for stressing 96 hours. During harvest in 10 months in 2015, selecting 100 tails of male shrimps with strong physique and good growth performance for individual seed reservation and overwintering, and counting the body length and the body weight of the male shrimps to serve as male parents of a grandparent of a matched system, wherein the body length of the male shrimps is 12.16-12.98cm, and the body weight of the male shrimps is 21.89-22.53 g.
(2) Randomly selecting 10 million shrimps from a group of Chinese prawns 'yellow sea No. 4', putting about 3.1 million survivors into an outdoor pond for cultivation after stressing for 96 hours under the condition that the pH value is 9.2. When the shrimps are harvested 10 months in 2015, 100 tails of the non-copulated female shrimps with strong physique and good growth performance are selected for individual seed reservation and overwintering, the length and the weight of the 100 tails are counted and used as female parents of a mating line ancestor, the length of the non-copulated female shrimps is 14.11-14.85cm, and the weight of the non-copulated female shrimps is 33.59-35.94 g.
Example 4: cultivation of specialized maternal lines
Selecting 30 healthy and strong female shrimps which are not copulated and male shrimps which are not copulated from the collected overwintering population of Chinese prawn 'yellow sea No. 1' in 2016, and hybridizing the shrimps in a ratio of 1:1 by adopting an artificial insemination technology. The parent shrimps after artificial insemination are independently put into a 200L polyethylene barrel for spawning and hatching. And putting the young shrimps of each family into an outdoor pond when the young shrimps grow to reach the body length of about 1.0cm, and culturing and testing under the same condition. Measuring the body length and the body weight of each family when harvested in 10 months in 2016, selecting 3-5 families with good performance, performing in-family selfing from F2 generations, and selecting the family with the best performance as a specialized maternal line through continuous 3-generation breeding, wherein the body length of the female shrimp is 16.07-17.14cm, and the body weight of the female shrimp is 47.63-55.12 g; the male shrimp has a length of 13.88-14.92cm and a weight of 31.36-35.63 g.
Example 5: culture of specialized paternal lines
In 2016, 30 healthy and strong male shrimps are selected from the overwintering population of the Chinese prawn, namely yellow sea No. 3, and 30 healthy and strong un-copulated females are selected from the overwintering population of the Chinese prawn, namely yellow sea No. 4, and are hybridized according to the ratio of 1:1 by adopting an artificial insemination technology. The parent shrimps after artificial insemination are independently put into a 200L polyethylene barrel for spawning and hatching. When the larvae of each family grow to young shrimps with the body length of about 1.0cm, 10 ten thousand of shrimps are randomly selected for ammonia nitrogen resistance and high pH value stress resistance experiments, the survival rate is controlled to be 3% -5%, and the shrimps are placed into an outdoor pond for cultivation. Measuring the body length and the weight of each family when harvested in 10 months in 2016, counting the success rate of cultivation, selecting 3-5 families with good performance, performing in-family selfing from the F2 generation, and selecting and cultivating the families with high growth speed and strong stress resistance as specialized father lines through continuous 3-generation breeding, wherein the high ammonia nitrogen and high pH resistance of the young shrimps is improved by 31.2 percent compared with that of the parents, the body length of the female shrimps is 14.07-15.04cm, and the weight of the female shrimps is 36.63-38.32 g; the male shrimp has a length of 13.18-14.09cm and a weight of 27.36-28.22 g.
Example 6: cultivation of Chinese prawn complete set line
The fast-growing stress-resistant Chinese prawn mating line commercial prawn is produced by hybridizing the specialized male line and the specialized female line, and has the advantages of strong physique, regular specification, stable hereditary character, and improved growth performance and stress resistance compared with the parent. The high ammonia nitrogen resistance and high pH value resistance of the young shrimps are improved by 21.6 percent compared with the ancestors, and the harvested female shrimps are 15.27 to 16.1cm in length and 43.16 to 46.33g in weight; the obtained male shrimp has a length of 13.38-14.39cm and a weight of 29.13-31.01 g. The whole technological process for breeding the Chinese prawn complete set line is shown in figure 1.
Claims (8)
1. A breeding method of a fast-growing stress-resistant Chinese prawn complete set line is characterized by comprising the following operation steps:
(1) collecting the 'yellow sea No. 1' group of Chinese prawn for pure reproduction, selecting individuals with excellent growth performance to construct a basic group, and using the basic group as a progenitor source of a mating line maternal line;
(2) collecting the 'yellow sea No. 3' and 'yellow sea No. 4' groups of the Chinese prawn for pure reproduction, respectively selecting individuals with strong high ammonia nitrogen and high pH stress tolerance capability in the aquaculture water body from the two groups to construct a basic group, and using the basic group as an ancestral source of a parent line of a mating line;
(3) selecting excellent female shrimp and male shrimp parents as ancestors from the pure breeding group of Chinese prawn 'yellow sea No. 1', hybridizing by adopting an artificial insemination technology, and breeding a family with high growth performance as a specialized maternal line;
(4) selecting male shrimp parents with strong high ammonia nitrogen stress resistance from the 'yellow sea No. 3' pure breeding population of Chinese prawns, selecting female shrimp parents with strong high pH stress resistance from the 'yellow sea No. 4' pure breeding population of Chinese prawns as ancestors, hybridizing by adopting an artificial insemination technology, and breeding a family with strong stress resistance as a specialized father line;
(5) selecting female shrimps from a specialized female line, selecting male shrimps from a specialized father line, and hybridizing by adopting an artificial insemination technology to obtain a fast-growing stress-resistant Chinese prawn mating line;
the group of the Chinese prawn, yellow sea No. 1, in the step (1) has the excellent character of high growth speed, and the registration number of the breed is GS 01001-2003;
the group of the Chinese prawn, yellow sea No. 3, in the step (2) has strong ammonia nitrogen stress resistance, and the variety registration number is GS-01-002-;
the Chinese prawn, yellow sea No. 4, has strong high pH value stress resistance, and the variety registration number is GS-01-003-.
2. The method of claim 1 wherein the selection criteria for female and male shrimp parents in step (3) are: the body constitution is strong, the appendages are complete, the body surface is smooth, the ovaries of the female shrimps grow well, the body length is more than 14cm, and the body weight is more than 37 g; the male shrimp is milk white, and has a length of more than 12cm and a weight of more than 24 g.
3. The method according to claim 2, wherein the method for breeding the specialized maternal line in step (3) comprises the following steps:
s1, selecting female shrimp and male shrimp parents with excellent growth characters, and hybridizing by adopting an artificial insemination technology according to a ratio of 1:1 to produce F1 generations;
s2, breeding 3-5 families with high growth speed from the F1 generation, and performing in-family selfing from the F2 generation;
s3, finally, selecting the family with the best growth character performance as a specialized maternal line through continuous breeding of 3 generations.
4. The method according to claim 3, wherein the parental selection criteria of the customized maternal line in step S3 are: the body constitution is strong, the appendages are complete, the body surface is smooth, the ovaries of the female shrimps grow well, the body length is more than 16cm, and the body weight is more than 47 g; the male shrimp is milk white, and has a length of more than 13cm and a weight of more than 31 g.
5. The method as claimed in claim 1, wherein the selection criteria of the male shrimp parent of the Chinese prawn "yellow sea No. 3" in step (4) are: the ammonium chloride concentration is 64mg/L, the shrimp survives under the condition of stress for 96 hours, the length of the male shrimp is more than 12cm, and the weight of the male shrimp is more than 21 g; the selection standard of female Chinese prawn "yellow sea No. 4" parent is as follows: the individuals surviving under the condition of stress of pH9.2 for 96h have the length of the un-copulated female shrimp larger than 14cm and the weight larger than 33 g.
6. The method according to claim 5, wherein the breeding method of the customized paternal line in the step (4) comprises the following steps:
s1, selecting male shrimps of the Chinese prawn 'yellow sea No. 3' and female shrimps of the Chinese prawn 'yellow sea No. 4', hybridizing by adopting an artificial insemination technology to produce F1 generations;
s2, breeding 3-5 families with strong high ammonia nitrogen resistance and high pH value stress resistance from the F1 generation, and performing in-family selfing from the F2 generation;
s3, finally, breeding the family with the strongest stress resistance as a specialized father line through continuous breeding of 3 generations.
7. The method according to claim 6, wherein the parental selection criteria of the specialized paternal line in step S3 are: compared with the parent generation, the high ammonia nitrogen resistance and high pH resistance of the young shrimps are improved by more than 30%, the ovaries of the female shrimps are full, the body length is more than 14cm, the weight is more than 36g, and the spermatophore of the male shrimps is milky white, the body length is more than 13cm, and the weight is more than 27 g.
8. The method of claim 1, wherein female shrimp is selected from the specialized maternal line of claim 4 and male shrimp is selected from the specialized paternal line of claim 7 and crossed to obtain commercial shrimp of good quality as a matched line.
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| CN115067280A (en) * | 2022-05-30 | 2022-09-20 | 上海市水产研究所（上海市水产技术推广站） | Three-line crossbreeding method for macrobrachium nipponense |
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| CN115067280A (en) * | 2022-05-30 | 2022-09-20 | 上海市水产研究所（上海市水产技术推广站） | Three-line crossbreeding method for macrobrachium nipponense |
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Application publication date: 20211123 |