CN103798165A - Good nacre cultivating method for pinctada martensii - Google Patents

Good nacre cultivating method for pinctada martensii Download PDF

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Publication number
CN103798165A
CN103798165A CN201410033634.7A CN201410033634A CN103798165A CN 103798165 A CN103798165 A CN 103798165A CN 201410033634 A CN201410033634 A CN 201410033634A CN 103798165 A CN103798165 A CN 103798165A
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shell
spat
breeding
cultivation
shells
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CN103798165B (en
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何毛贤
林坚士
刘文广
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South China Sea Institute of Oceanology of CAS
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South China Sea Institute of Oceanology of CAS
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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Abstract

The invention discloses a good nacre cultivating method for pinctada martensii. The method comprising the flowing steps: (1) from a certain wild pinctada martensii group, selecting individuals which are larger than 20mm in shell width and larger than 0.35 in ratio of the shell width and the shell height as breeding parents; (2) from the breeding parents, selecting more than 30 female individuals with mature sexual glands and more than 20 male individuals, taking sperms and eggs through cutting, conducting artificial insemination, and breeding spat young shells; (3) consecutively selecting parents according to the step (1) and breeding spat young shells according to the step (2) for more than three generations when bred spat young shells reach 1.5-2.0 years. Consequently, obtained spat young shells can be used for large-scale production breeding and cultivation. The good nacre cultivating method for the pinctada martensii can remarkably improve the forms of shells and enable the shell to be thick, is suitable for core insertion pearl cultivation, and can reduce death and improve pearl cultivation characters.

Description

A kind of breeding method of pteria martensii excellent pinctada
Technical field:
The invention belongs to aquaculture and breeding field, be specifically related to a kind of breeding method of pteria martensii excellent pinctada.
Background technology:
Pteria martensii is China and the main species of sea water pearls cultivation in the world.China is since nineteen sixty-five has successfully been carried out pteria martensii artificial breeding, and sea water pearls industry development is rapid, has become one of Guangdong, Guangxi and Hainan part coastal area mariculture pillar industry.Nearly more than 10 years, the Quality and yield of China's sea water pearls obviously declined, and international competitiveness weakens, and fanning economics glides.Trace it to its cause, except breeding environment worsen, cultural technique fall behind, also have a major reason be: do not focus on for many years seed selection and the reservation of kind of shellfish, blind import denizen is also hybridized, cause germplasm mix, impure.At present the cultured population in Zhu Yang district mostly is crossbreed, although this shellfish growth is fast, mytiliform is more flat, soft body is modest, insert core after lethality high.For the healthy and stable development of China's sea water pearls aquaculture, introduce not extremely urgent by the pteria martensii good seed of China's Natural Population of genetic pollution the applicable nucleus insertion and pearl of cultivation.
Improvement to pteria martensii, mainly focuses on the color of growth rate or shell etc., and also there is no relevant report for the selection of hull shape at present.
Summary of the invention:
The object of this invention is to provide a kind of remarkable form of improving shellfish, be suitable for nucleus insertion and pearl and reduce the breeding method of dead pteria martensii excellent pinctada.
The breeding method of pteria martensii excellent pinctada of the present invention, comprises the following steps:
(1), in a certain colony of wild pteria martensii, be wider than 20mm with shell, simultaneously shell wide/shell is high breeds parent than the system of selection that the is greater than 0.35 individual conduct of selecting and remain;
(2), from breeding parent, select the female individuals of gonad maturity more than 30, male more than 20, cuts open and gets smart ovum, artificial insemination, cultivate spat;
(3), in the time of the shellfish in age of cultivated spat to 1.5~2.0, according to the system of selection of step (1) parent that selects and remain, cultivate spat according to the method for step (2), so more than 3 generations of successive selection, the spat of acquisition can be used for that large-scale production is cultivated and cultivation.
Described wild pteria martensii, preferably 1.5~2.0 age shellfish wild pteria martensii.
Compared with prior art, the present invention has following beneficial effect:
Take wild pteria martensii colony as core population, by the breeding method of pteria martensii excellent pinctada of the present invention, the form that can significantly improve shellfish makes its hull shape thick, is suitable for nucleus insertion and pearl, reduce dead, thereby improve the proterties of growing cultured pearls.
Embodiment:
Following examples are to further illustrate of the present invention, rather than limitation of the present invention.
Embodiment 1:
In April, 2010, select take the first generation core population that derives from the wild pteria martensii in Shenzhen as the cultivation of selecting colony to carry out the second generation, shellfish 1.5 ages of age, 1500 of quantity, shell height and the shell of measuring each individuality are wide, calculate shell wide/the high ratio of shell, select ratio be greater than 0.35 and the shell individuality that is wider than 20mm be parent, after slaking is cultivated, therefrom select 32 of the female individuals of gonad maturity, 56 of males, dissect shellfish and get its sexual gland, the smart ovum of collecting is through silk cover filtering, and rapidly ovum is added in the flat aquarium of 100L of the ammonia seawater that fills 0.005%, then add appropriate sperm, and mix and allow its fertilization, it is 24 ℃ that water temperature is grown in insemination.The shell that measures its offspring in September, 2013 is wide/and shell is high than being 0.351, and and cultivation control population is 0.339, and shell is wide/and shell is high than increase 3.54%; In November, 2013, its shell is wide/shell is high than being 0.352, and cultivation control population is 0.332, and shell is wide/and shell is high than increase 6.02%, and hull shape is obviously thick compared with control population.
In April, 2012, cultivate the third generation with the second generation, shellfish 2.0 ages of age, 2000 of quantity, shell height and the shell of measuring each individuality are wide, calculate shell wide/the high ratio of shell, select ratio be greater than 0.35 and the shell individuality that is wider than 20mm be parent, after slaking is cultivated, therefrom select 45 of the female individuals of gonad maturity, 43 of males, dissect shellfish and get its sexual gland, and the smart ovum of collection is through silk cover filtering, and rapidly ovum is added in the flat aquarium of 100L of the ammonia seawater that fills 0.005%, then add appropriate sperm, and mix allow its fertilization, insemination grow water temperature be 22 ℃.The shell that measures its offspring in June, 2013 is wide/and shell is high than being 0.347, and and cultivation control population is 0.308, and shell is wide/and shell is high than increase 12.66%; In September, 2013, its shell is wide/shell is high than being 0.359, and cultivation control population is 0.337, and shell is wide/and shell is high than increase 6.53%, and hull shape is obviously thick compared with control population.
Carry out nucleus insertion and pearl with the pteria martensii colony by breeding method seed selection of the present invention.After after slotting core 1 month finishes, lethality is added up.This colony's lethality is 0~18.42%, average 9.74%; Control group is 7.89~21.05%, average out to 15.79%, and significant difference p<0.05, lethality has reduced by 38.32%.

Claims (2)

1. a breeding method for pteria martensii excellent pinctada, is characterized in that, comprises the following steps:
(1), in a certain colony of wild pteria martensii, be wider than 20mm with shell, simultaneously shell wide/shell is high breeds parent than the system of selection that the is greater than 0.35 individual conduct of selecting and remain;
(2), from breeding parent, select the female individuals of gonad maturity more than 30, male more than 20, cuts open and gets smart ovum, artificial insemination, cultivate spat;
(3) in the time of the shellfish in age of cultivated spat to 1.5~2.0, according to the system of selection of step (1) parent that selects and remain, cultivate spat according to the method for step (2), so more than 3 generations of successive selection, the spat of acquisition can be used for that large-scale production is cultivated and cultivation.
2. the breeding method of pteria martensii excellent pinctada according to claim 1, is characterized in that, described wild pteria martensii be 1.5~2.0 age shellfish.
CN201410033634.7A 2014-01-23 2014-01-23 A kind of breeding method of pteria martensii excellent pinctada Active CN103798165B (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104099415A (en) * 2014-06-24 2014-10-15 中国科学院南海海洋研究所 Detection primer marked by SNP and associated with pinctada martensi adductor muscle weight and application of detection primer
CN104855311A (en) * 2015-05-08 2015-08-26 大连海洋大学 Seed production method for new lines of wide-shell-type ruditapes philippinarum
CN105265351A (en) * 2014-07-23 2016-01-27 北海市铁山港区生产力促进中心 Seawater pearl culture method
CN111972331A (en) * 2020-08-31 2020-11-24 北部湾大学 Method for cultivating bead-breeding receptor pinctada fucata martensii

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102388828A (en) * 2011-10-09 2012-03-28 中国科学院南海海洋研究所 Breeding method for rapid growing line of seawater pearl shells
CN102960271A (en) * 2012-11-09 2013-03-13 中国水产科学研究院南海水产研究所 Rotational mating breeding method for pinctada fucata groups

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102388828A (en) * 2011-10-09 2012-03-28 中国科学院南海海洋研究所 Breeding method for rapid growing line of seawater pearl shells
CN102960271A (en) * 2012-11-09 2013-03-13 中国水产科学研究院南海水产研究所 Rotational mating breeding method for pinctada fucata groups

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
何毛贤等: "马氏珠母贝选育子一代生长特性研究", 《热带海洋学报》, vol. 25, no. 1, 30 January 2006 (2006-01-30) *
刘志刚等: "马氏珠母贝经济性状对体重决定效应分析", 《广东海洋大学学报》, vol. 27, no. 4, 15 August 2007 (2007-08-15) *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104099415A (en) * 2014-06-24 2014-10-15 中国科学院南海海洋研究所 Detection primer marked by SNP and associated with pinctada martensi adductor muscle weight and application of detection primer
CN104099415B (en) * 2014-06-24 2015-09-23 中国科学院南海海洋研究所 A kind of detection primer of the SNP marker associated with pteria martensii closed shell flesh heavy phase and application thereof
CN105265351A (en) * 2014-07-23 2016-01-27 北海市铁山港区生产力促进中心 Seawater pearl culture method
CN104855311A (en) * 2015-05-08 2015-08-26 大连海洋大学 Seed production method for new lines of wide-shell-type ruditapes philippinarum
CN111972331A (en) * 2020-08-31 2020-11-24 北部湾大学 Method for cultivating bead-breeding receptor pinctada fucata martensii

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