CN111066648A - Method for promoting formation of sporocyst branches of shells protonema of half-leaf laver - Google Patents
Method for promoting formation of sporocyst branches of shells protonema of half-leaf laver Download PDFInfo
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Abstract
The invention relates to a method for promoting formation of sporocyst branches of shells protonema of half-leaf laver, belonging to the field of marine algae culture, comprising the following steps: 1) collecting semilaver fruit spores; 2) inoculating shell with half-leaf laver fruit spore; 3) promoting the algae filaments to grow rapidly in the algae filament vegetative growth stage of the shell filaments; 4) when most of nutritious algae filaments of the half-leaf laver shell filament are fully distributed on the shell and sporangium branches begin to appear, the concentration of iron ions is increased to induce the algae filaments to be converted from a vegetative growth state to a development state, so that the sporangium branches are quickly formed; the method can promote the rapid growth of the nutrient phycofilaments of the shell protonema of the half-leaf laver, synchronously form sporangium branches in a short time under the condition of no temperature rise, and reduce the risks of diseases such as bacterial infection, mass attachment of miscellaneous algae and the like of the shell under the relatively low water temperature environment, thereby improving the success rate of the artificial seedling culture of the shell protonema of the half-leaf laver.
Description
Technical Field
The invention belongs to the field of marine algae culture, and particularly relates to a method for promoting formation of sporocyst branches of shells protonema of half-leaf laver.
Background
The laver has higher nutritive value and economic value, and is one of the most important cultivated seaweeds in China. The laver cultivation method in China includes vegetable jar cultivation, strut type cultivation, semi-floating raft type cultivation, full-floating raft type cultivation and the like. The vegetable jar has limited area, natural sources of seedlings and easy influence of sea conditions and meteorological conditions, so that the vegetable jar has rare cultivation modes. The existing pillar type cultivation and semi-floating raft type cultivation modes are widely applied to porphyra yezoensis and porphyra haitanensis cultivation industries, and the method is applied to intertidal zones or offshore zones, can lead the porphyra to be dried out periodically, and is beneficial to removing miscellaneous algae and growing the porphyra. In recent years, with the increasing demand of seaweed food markets for laver products such as laver and the like, the laver cultivation industry is rapidly developing, and the intertidal zone or the offshore zone suitable for laver cultivation is increasingly saturated. Driven by economic interest, some intertidal sea areas are subjected to high-density cultivation of laver, resulting in severe deterioration of quality and yield of laver and frequent occurrence of diseases. The full-floating raft type cultivation method can utilize the vast space of open sea, can relieve the space problem of laver cultivation, but has the defect that the drying time of a laver net curtain is limited, so that the laver is easily polluted by attachment of miscellaneous algae. In order to make the full-floating raft type cultivation method widely applied, in addition to further perfecting the full-floating raft type cultivation technology and equipment, the development of a new laver cultivation variety is also one of more effective measures. The thickness, color and taste of the half-leaf laver (Pyropia katadae) are similar to those of the porphyra yezoensis, the half-leaf laver (Pyropia katadae) naturally grows in low-lying marshlands in intertidal zones, the anti-attachment capacity of the hybrid algae is strong, and the natural life habit of the half-leaf laver is generally not like that of the porphyra yezoensis and the porphyra haizoensis which need to be dried out periodically, so that the half-leaf laver (Pyropia katadae) is probably more suitable for being applied to a full-floating raft type cultivation mode and is a potential excellent algae species developed to. However, the existing seedling cultivation technology of half-leaf laver is not mature, and the application of the half-leaf laver in laver production is limited.
The seedling cultivation of laver is mainly to culture the filament, while the filament for seedling cultivation usually grows on the calcareous solid (such as concha meretricis seu cyclinae, oyster shell, clam shell, etc.), and the production can utilize the plane or hanging cultivation of shell filament in the big water pond after the laver fruit spore or free filament is drilled into the shell. The process of the growth and development of the shell protonema can be generally divided into four stages: vegetative growth stage, sporangium branch forming stage, conchospore forming stage and conchospore diffusing stage. Temperature, salinity, light intensity, light period, nutrient salt and the like are all key ecological factors influencing the growth and development of the shell protonema. Under normal conditions, when the shell protonema enters a developmental state from a vegetative growth state (sporangial branch formation), the temperature is generally increased, and the sporangial branch formation is slow. For example, in the artificial seedling production of the shell filament of porphyra yezoensis in Jiangsu, the shell filament is cultivated from late 3 months or early 4 months, and sporangial branches generally begin to appear from late 6 months to early 7 months as the water temperature gradually rises. Under normal culture conditions, the forming rate of the sporocyst branches of the shell protonema of the porphyra yezoensis is 20-30% at the beginning of 8 months, 40-50% at the bottom of 8 months and 60-80% at the beginning of 9 months, namely, about 60 days are needed from the appearance of the sporocyst branches to the formation rate of the sporocyst branches reaching about 50%. Since the concentrated formation stage (7-8 months) of the shell protonema sporocyst branches of the porphyra yezoensis is in an environment with relatively high water temperature for a long time (generally about 25 ℃, and the water temperature sometimes reaches 27-29 ℃ in south Jiangsu), the problems that bacteria are easily bred on the shells, the adhesion of other algae and other diseases occur on the shells, the shells and a culture pond need to be washed frequently, and more manpower, material resources and financial resources are consumed. Therefore, if the limit of temperature rise can be eliminated and the sporangium branch can be rapidly formed from the development of the nutrient algae filament to the sporangium branch, the method has important economic significance for the artificial seedling raising of the laver shell filament.
There are a number of studies and patents on the growth and development of porphyra yezoensis or porphyra haitanensis filaments. For example, chinese patent CN109906934A discloses a method for promoting the formation of sporocyst branches of porphyra yezoensis filaments, which utilizes a relatively high concentration of carbon dioxide or bicarbonate ions to increase the rate of formation of sporocyst branches of porphyra yezoensis free filaments; in patent CN105145329A, a method for promoting maturation and collecting seedlings of Porphyra haitanensis shell filaments is reported, wherein conchospores of Porphyra haitanensis shell filaments can be matured and synchronously diffused by means of adjustment of phosphate fertilizer ratio, adjustment of light intensity and photoperiod, addition of calcium chloride ions and the like; as a result of research on the effect of different concentrations and forms of phosphorus on Porphyra haitanensis sporangial shoot formation, it was suggested that inorganic phosphate promotes Porphyra haitanensis sporangial shoot formation more than organic phosphate.
However, there are few reports on the research on the growth and development of the thallus Porphyrae semialata filaments. Chinese patent CN103798123B discloses a method for culturing seedlings of half-leaf laver, which aims at the free filament of half-leaf laver rather than shell filament, and realizes the seedling culture of the free filament of half-leaf laver by regulating and controlling temperature, light intensity, photoperiod and seawater specific gravity. For the research on the thallus Porphyrae semilobae shell protonema, only Tongrong et al (1999) studied the effect of temperature on maturation, diffusion conditions and growth and development of seedlings of thallus Porphyrae conchospores, and found that sporocyst branches of thallus Porphyrae semilobae shells protonema are formed in large quantities when the temperature is raised to 26-31 ℃. In addition to the above studies, no report on how to regulate the formation of sporocyst branches of the thallus Porphyrae semialae shell filaments is known.
Disclosure of Invention
The invention aims to provide a method for promoting formation of sporocyst branches of shells of half-leaf laver protonema.
The invention is realized by the following technical scheme:
a method of promoting formation of sporocyst branches of laver semileaflet shells protonema, the method comprising the steps of: 1) collecting semilaver fruit spores; 2) inoculating shell with half-leaf laver fruit spore; 3) promoting the algae filaments to grow rapidly in the algae filament vegetative growth stage of the shell filaments; 4) when most of nutritious algae filaments of the half-leaf laver shell filament are fully distributed on the shell and sporangium branches begin to appear, the concentration of iron ions is increased to induce the algae filaments to be converted from a vegetative growth state to a development state, so that the sporangium branches are quickly formed;
collecting high-quality half-leaf laver, drying in the shade, stimulating, and adding seawater to promote release of fruit spores to obtain fruit spore water;
further, inoculating the shell with the fruit spores of the half-leaf laver in the step 2), and putting the shell with the density of 150-250 fruit spores/cm2。
Further, the method for inoculating the shell with the fruit spores comprises the steps of measuring out required fruit spore water, filling the fruit spore water into a spraying pot, uniformly spraying the fruit spore water into a culture pond, naturally settling the fruit spores on the shell, adjusting the illumination intensity in the culture condition to be within the range of 1500-2500 lx, adding nutrient salt into seawater with the concentration of 2-4 mg/L of inorganic nitrogen and 0.2-0.4 mg/L of inorganic phosphorus, controlling the water temperature to be 15-20 ℃, sampling after fruit spores are picked for one week, checking the germination rate of the fruit spores, and changing water and washing when the germination rate of the fruit spores reaches 40-70%;
the step 3) of promoting the algae filaments to rapidly grow in the algae filament vegetative growth stage of the shell filaments comprises the following steps of (1) controlling proper environmental conditions: the illumination intensity is controlled to be 2000-3000 lx, the illumination time is more than 12 hours per day, the concentration of nutrient salt added into the seawater is 5.0-10.0 mg/L of inorganic nitrogen, 0.5-1.0 mg/L of inorganic phosphorus and 0.01-0.05 mg/L of iron ions, and the water temperature is controlled to be 18-22 ℃; (2) water changing and washing measures: replenishing fresh seawater every day, changing water every week, and washing shells and the culture pond every two weeks;
step 4), when most of nutritious algae filaments of the half-leaf laver shell filament body are fully distributed on the shell and sporangium branches begin to appear, increasing the concentration of iron ions to induce the algae filaments to be converted from a vegetative growth state to a development state, and enabling the sporangium branches to quickly form a shape including (1) the concentration of the iron ions in seawater is increased to 0.2-2.5 mg/L; (2) the illumination time is controlled to be 10-12 h every day, the illumination intensity is controlled to be 1500-2500 lx, and the inorganic nitrogen concentration, the inorganic phosphorus concentration and the water temperature are controlled to be consistent with those in the step 3); (3) water changing and washing measures: supplementing some fresh seawater every day, changing water every week, and washing shells and the culture pond every two weeks.
Compared with the prior art, the invention has the following beneficial effects:
the invention can promote the rapid growth of the nutrient algae filaments of the shell filaments of the half-leaf laver by controlling the conditions of light, temperature and nutrient salts (nitrogen, phosphorus and iron) at different growth and development stages of the shell filaments of the half-leaf laver, particularly controlling the condition of iron concentration to carry out development regulation and control on the half-leaf laver shell filaments, and the nutrient algae filaments can synchronously form sporangium branches in a short time under the condition of not increasing temperature. On the premise of perfecting the semi-leaf laver seedling collection technology, the rapid formation of the sporangium branches can reduce the production cost, improve the seedling collection efficiency, ensure that semi-leaf laver shell protonema with synchronous development is obtained timely, and meet the requirements of basic research and production application. The risk of diseases such as bacterial infection, a large amount of attached foreign algae and the like of the shell can be reduced under the relatively low water temperature environment, so that the success rate of artificial seedling culture of the semi-laver shell protonema is improved.
Detailed Description
The technical solution of the present invention is further explained by the following examples, but the scope of the present invention is not limited in any way by the examples.
Example 1
1) Selecting healthy algae with larger individual, deeper color and higher maturity from wild half-leaf laver as seed algae, fully cleaning with sterilized natural seawater, spreading the seed algae on a bamboo curtain after squeezing water, drying in the shade to lose about 50% of water, putting the seed algae after being dried in the shade and stimulated into a container containing the sterilized natural seawater (100 kg of water is added into each kg of the seed algae), continuously stirring the seawater to promote release of fruit spores, taking a water sample for microscopic examination, fishing out the seed algae when the number of the fruit spores is 10-20 under the 100-fold magnification, and filtering with a 200-mesh bolting silk to obtain a filtrate, namely the fruit spore water.
2) Counting the number of fruit spores contained in each milliliter of semi-leaf laver fruit spore water by using a phytoplankton counting frame, calculating to obtain the concentration of the fruit spore water, and obtaining the concentration of the fruit spores according to the area of a culture pond and 200 fruit spores/cm2The total number of the fruit spores to be put and the dosage of the fruit spore water are calculated. Measuring the needed fruit spore water by using a measuring cylinder, filling the fruit spore water into a spraying pot, and uniformly spraying the fruit spore water into the culture pond to ensure that the fruit spores are naturally settled on the shells. The culture conditions were: illuminance of 2000lx, inorganic nitrogen concentration of 3mg/L, inorganicThe phosphorus concentration was 0.3mg/L and the water temperature was 18 ℃. After picking fruit spores for one week, sampling and checking to find that the germination rate of the fruit spores reaches 50%, and changing water and washing.
3) In the vegetative growth stage of the algae filaments of the shell filaments of the half-leaf laver, the culture conditions are as follows: the illumination intensity is 2500lx, the illumination time is 12h every day, the inorganic nitrogen concentration is 8.0mg/L, the inorganic phosphorus concentration is 0.8mg/L, the iron ion concentration is 0.03mg/L, and the water temperature is controlled at 20 ℃. Supplementing some fresh seawater every day, changing water every week, and washing shells and the culture pond every two weeks. Every 7 days, shells were taken to observe the growth of the filament.
4) And 60 days after fruit spores are picked, the nutrient algae filaments of the half-leaf laver shell filaments on most of the shells are fully distributed on the shells and sporangium branches begin to appear, and the concentration of iron ions in seawater is increased to 1mg/L so as to induce the nutrient algae filaments to synchronously develop and enable the sporangium branches to be quickly formed. Other incubation conditions were: the water temperature is 20 ℃, the illumination time is 10-12 h per day, the illumination intensity is 2000lx, the inorganic nitrogen concentration is 10.0mg/L, and the inorganic phosphorus concentration is 1.0 mg/L. Supplementing some fresh seawater every day, changing water every week, and washing shells and the culture pond every two weeks.
This example gives the following results: the algae filament biomass of the half-leaf laver shell filament can meet the seedling collecting requirement within 60 days, and only about 40 days are needed from the appearance of the sporangium to the formation rate of the sporangium of 50 percent under the condition of no temperature rise through the development induction of iron ions. In the traditional cultivation method, the nutrition algae filaments of the shell protonema need to be cultivated for about 90 days to meet the seedling collection requirement, the temperature needs to be increased to induce the formation of the sporangium branch, and about 60 days are needed from the appearance of the sporangium branch to the 50 percent formation rate of the sporangium branch. Therefore, the growth speed of the nutritious algal filaments of the shell protonema of the half-leaf laver and the formation speed of the induced sporangium branches are obviously improved.
Example 2
1) Selecting healthy algae with larger individual, deeper color and higher maturity from wild half-leaf laver as seed algae, fully cleaning with sterilized natural seawater, spreading the seed algae on a bamboo curtain after squeezing water, drying in the shade to lose about 50% of water, putting the seed algae after being dried in the shade and stimulated into a container containing the sterilized natural seawater (100 kg of water is added into each kg of the seed algae), continuously stirring the seawater to promote release of fruit spores, taking a water sample for microscopic examination, fishing out the seed algae when the number of the fruit spores is 10-20 under the 100-fold magnification, and filtering with a 300-mesh bolting silk to obtain a filtrate, namely the fruit spore water.
2) Counting the number of fruit spores contained in each milliliter of semi-leaf laver fruit spore water by using a phytoplankton counting frame, calculating to obtain the concentration of the fruit spore water, and calculating according to the area of a culture pond and 150 fruit spores/cm2The total number of the fruit spores to be put and the dosage of the fruit spore water are calculated. Measuring the needed fruit spore water by using a measuring cylinder, filling the fruit spore water into a spraying pot, and uniformly spraying the fruit spore water into the culture pond to ensure that the fruit spores are naturally settled on the shells. The culture conditions were: the illumination intensity is 1500lx, the inorganic nitrogen concentration is 2mg/L, the inorganic phosphorus concentration is 0.2mg/L, and the water temperature is 15 ℃. After picking fruit spores for one week, sampling and checking to find that the germination rate of the fruit spores reaches 40%, and changing water and washing.
3) In the vegetative growth stage of the algae filaments of the shell filaments of the half-leaf laver, the culture conditions are as follows: the illumination intensity is 2000lx, the illumination time is 12h every day, the inorganic nitrogen concentration is 5.0mg/L, the inorganic phosphorus concentration is 0.5mg/L, the iron ion concentration is 0.01mg/L, and the water temperature is controlled at 18 ℃. Supplementing some fresh seawater every day, changing water every week, and washing shells and the culture pond every two weeks. Every 7 days, shells were taken to observe the growth of the filament.
4) And on the 70 th day after fruit spores are picked, nutrient algae filaments of the half-leaf laver shell filaments on most of the shells are fully distributed on the shells and sporangium branches begin to appear, and the concentration of iron ions in the seawater is increased to 0.2mg/L so as to induce the nutrient algae filaments to synchronously develop and enable the sporangium branches to be quickly formed. Other incubation conditions were: the water temperature is 18 ℃, the illumination time is 10 hours per day, the illumination intensity is 2000lx, the inorganic nitrogen concentration is 5.0mg/L, and the inorganic phosphorus concentration is 0.5 mg/L. Supplementing some fresh seawater every day, changing water every week, and washing shells and the culture pond every two weeks.
This example gives the following results: the algae filament biomass of the half-leaf laver shell filament can meet the seedling collecting requirement within 70 days, and only about 50 days are needed from the appearance of the sporangium to the formation rate of the sporangium of 50 percent under the condition of no temperature rise through the development induction of iron ions. In the traditional cultivation method, the nutrition algae filaments of the shell protonema need to be cultivated for about 90 days to meet the seedling collection requirement, the temperature needs to be increased to induce the formation of the sporangium branch, and about 60 days are needed from the appearance of the sporangium branch to the 50 percent formation rate of the sporangium branch. Therefore, the growth speed of the nutritious algal filaments of the shell protonema of the half-leaf laver and the formation speed of the induced sporangium branches are obviously improved.
Example 3
1) Selecting healthy algae with larger individual, deeper color and higher maturity from wild half-leaf laver as seed algae, fully cleaning with sterilized natural seawater, spreading the seed algae on a bamboo curtain after squeezing water, drying in the shade to lose about 50% of water, putting the seed algae after being dried in the shade and stimulated into a container containing the sterilized natural seawater (100 kg of water is added into each kg of the seed algae), continuously stirring the seawater to promote release of fruit spores, taking a water sample for microscopic examination, fishing out the seed algae when the number of the fruit spores is 10-20 under the 100-fold magnification, and filtering with a 500-mesh bolting silk to obtain a filtrate, namely the fruit spore water.
2) Counting the number of fruit spores contained in each milliliter of semi-leaf laver fruit spore water by using a phytoplankton counting frame, calculating to obtain the concentration of the fruit spore water, and calculating according to the area of a culture pond and 250 fruit spores/cm2The total number of the fruit spores to be put and the dosage of the fruit spore water are calculated. Measuring the needed fruit spore water by using a measuring cylinder, filling the fruit spore water into a spraying pot, and uniformly spraying the fruit spore water into the culture pond to ensure that the fruit spores are naturally settled on the shells. The culture conditions were: the illumination intensity is 2500lx, the inorganic nitrogen concentration is 4mg/L, the inorganic phosphorus concentration is 0.4mg/L, and the water temperature is 20 ℃. After picking fruit spores for one week, sampling and checking to find that the germination rate of the fruit spores reaches 70 percent, and changing water and washing.
3) In the vegetative growth stage of the algae filaments of the shell filaments of the half-leaf laver, the culture conditions are as follows: the illumination intensity is 3000lx, the illumination time is 15h every day, the inorganic nitrogen concentration is 10.0mg/L, the inorganic phosphorus concentration is 1.0mg/L, the iron ion concentration is 0.05mg/L, and the water temperature is controlled at 22 ℃. Supplementing some fresh seawater every day, changing water every week, and washing shells and the culture pond every two weeks. Every 7 days, shells were taken to observe the growth of the filament.
4) And 54 days after fruit spores are picked, the nutrient algae filaments of the half-leaf laver shell filaments on most of the shells are fully distributed on the shells and sporangium branches begin to appear, and the concentration of iron ions in the seawater is increased to 2.5mg/L so as to induce the nutrient algae filaments to synchronously develop and enable the sporangium branches to be quickly formed. Other incubation conditions were: the water temperature is 22 ℃, the illumination time is 12h per day, the illumination intensity is 2500lx, the inorganic nitrogen concentration is 10.0mg/L, and the inorganic phosphorus concentration is 1.0 mg/L. Supplementing some fresh seawater every day, changing water every week, and washing shells and the culture pond every two weeks.
This example gives the following results: the algae filament biomass of the half-leaf laver shell filament can meet the seedling collecting requirement within 54 days, and only about 45 days are needed from the appearance of the sporangium to the formation rate of the sporangium of 50 percent under the condition of no temperature rise through the development induction of iron ions. In the traditional cultivation method, the nutrition algae filaments of the shell protonema need to be cultivated for about 90 days to meet the seedling collection requirement, the temperature needs to be increased to induce the formation of the sporangium branch, and about 60 days are needed from the appearance of the sporangium branch to the 50 percent formation rate of the sporangium branch. Therefore, the growth speed of the nutritious algal filaments of the shell protonema of the half-leaf laver and the formation speed of the induced sporangium branches are obviously improved.
Claims (3)
1. A method of promoting formation of sporocyst branches of laver semileaflet shells protonema, the method comprising the steps of: 1) collecting semilaver fruit spores; 2) inoculating shell with half-leaf laver fruit spore; 3) promoting the algae filaments to grow rapidly in the algae filament vegetative growth stage of the shell filaments; 4) when most of nutritious algae filaments of the half-leaf laver shell filament are fully distributed on the shell and sporangium branches begin to appear, the concentration of iron ions is increased to induce the algae filaments to be converted from a vegetative growth state to a development state, so that the sporangium branches are quickly formed; the method is characterized in that:
the step 3) of promoting the algae filaments to rapidly grow in the algae filament vegetative growth stage of the shell filaments comprises the following steps of (1) controlling proper environmental conditions: the illumination intensity is controlled to be 2000-3000 lx, the illumination time is more than 12 hours per day, the concentration of nutrient salt added into the seawater is 5.0-10.0 mg/L of inorganic nitrogen, 0.5-1.0 mg/L of inorganic phosphorus and 0.01-0.05 mg/L of iron ions, and the water temperature is controlled to be 18-22 ℃; (2) water changing and washing measures: replenishing fresh seawater every day, changing water every week, and washing shells and the culture pond every two weeks;
step 4), when most of nutritious algae filaments of the half-leaf laver shell filament body are fully distributed on the shell and sporangium branches begin to appear, increasing the concentration of iron ions to induce the algae filaments to be converted from a vegetative growth state to a development state, and enabling the sporangium branches to quickly form a shape including (1) the concentration of the iron ions in seawater is increased to 0.2-2.5 mg/L; (2) the illumination time is controlled to be 10-12 h every day, the illumination intensity is controlled to be 1500-2500 lx, and the inorganic nitrogen concentration, the inorganic phosphorus concentration and the water temperature are controlled to be consistent with those in the step 3); (3) water changing and washing measures: supplementing some fresh seawater every day, changing water every week, and washing shells and the culture pond every two weeks.
2. The method according to claim 1, wherein the step 2) inoculating the shell with fruit spores of half-leaf laver, the throwing density is 150-250 fruit spores/cm2。
3. The method as claimed in claim 1, wherein the method for inoculating the shell with the fruit spores of the half-leaf laver in the step 2) comprises the steps of measuring out the required fruit spore water, putting the fruit spore water into a sprinkling can, uniformly sprinkling the fruit spore water into a culture pond, naturally settling the fruit spores on the shell, adjusting the illumination intensity in the culture condition to be within the range of 1500-2500 lx, adding nutrient salt into seawater with the concentration of 2-4 mg/L of inorganic nitrogen and 0.2-0.4 mg/L of inorganic phosphorus, controlling the water temperature to be 15-20 ℃, sampling after picking the fruit spores for one week, checking the germination rate of the fruit spores, and changing water and washing when the germination rate of the fruit spores reaches 40-70%.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112425503A (en) * | 2020-12-10 | 2021-03-02 | 江苏海洋大学 | Device for full-artificial seedling picking of asparagus |
| CN114747476A (en) * | 2021-03-12 | 2022-07-15 | 中国水产科学研究院黄海水产研究所 | Method for promoting formation of sea-tangle sporangium |
| CN118318723A (en) * | 2024-04-25 | 2024-07-12 | 宁波大学 | Industrial laver cultivation system and cultivation method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5995828A (en) * | 1982-11-22 | 1984-06-02 | 明治製菓株式会社 | Laver samplng method |
| CN103798123A (en) * | 2014-02-19 | 2014-05-21 | 中国水产科学研究院黄海水产研究所 | Semi-leaf laver seedling cultivating method |
| CN103931482A (en) * | 2014-04-26 | 2014-07-23 | 宁波大学 | Porphyra haitanensis shell conchocelis ripening and seedling collecting method |
-
2019
- 2019-12-30 CN CN201911394593.3A patent/CN111066648B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5995828A (en) * | 1982-11-22 | 1984-06-02 | 明治製菓株式会社 | Laver samplng method |
| CN103798123A (en) * | 2014-02-19 | 2014-05-21 | 中国水产科学研究院黄海水产研究所 | Semi-leaf laver seedling cultivating method |
| CN103931482A (en) * | 2014-04-26 | 2014-07-23 | 宁波大学 | Porphyra haitanensis shell conchocelis ripening and seedling collecting method |
Non-Patent Citations (3)
| Title |
|---|
| 山东省水产学校: "《实用海水藻类养殖技术》", 31 August 1985 * |
| 张光贵等: "紫菜育苗工艺改进技术", 《科学养鱼》 * |
| 石斌等: "条斑紫菜贝壳丝状体培育技术", 《齐鲁渔业》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112425503A (en) * | 2020-12-10 | 2021-03-02 | 江苏海洋大学 | Device for full-artificial seedling picking of asparagus |
| CN112425503B (en) * | 2020-12-10 | 2022-04-08 | 江苏海洋大学 | Device for full-artificial seedling picking of asparagus |
| CN114747476A (en) * | 2021-03-12 | 2022-07-15 | 中国水产科学研究院黄海水产研究所 | Method for promoting formation of sea-tangle sporangium |
| CN118318723A (en) * | 2024-04-25 | 2024-07-12 | 宁波大学 | Industrial laver cultivation system and cultivation method |
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