CN112450058B - Production method of peony for selenium-enriched oil - Google Patents

Production method of peony for selenium-enriched oil Download PDF

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CN112450058B
CN112450058B CN202011254095.1A CN202011254095A CN112450058B CN 112450058 B CN112450058 B CN 112450058B CN 202011254095 A CN202011254095 A CN 202011254095A CN 112450058 B CN112450058 B CN 112450058B
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selenium
peony
soil
oil
seeds
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CN112450058A (en
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王在贵
聂铭
聂琬晴
聂蕾
房月华
聂荣京
马嵩
高家磊
卞忠义
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Tongling Fengdan Planting Technology Development Co ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A01G24/23Wood, e.g. wood chips or sawdust
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A01G24/25Dry fruit hulls or husks, e.g. chaff or coir
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/30Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds
    • A01G24/35Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds containing water-absorbing polymers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers
    • C05D9/02Other inorganic fertilisers containing trace elements
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F1/00Fertilisers made from animal corpses, or parts thereof
    • C05F1/007Fertilisers made from animal corpses, or parts thereof from derived products of animal origin or their wastes, e.g. leather, dairy products
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The application discloses a production method of peony for selenium-enriched oil, which comprises the following steps: mixing sodium selenite aqueous solution into faeces Bombycis, adding compound bacteria, stirring, and sealing for fermentation to obtain selenium-rich fertilizer; selecting a soil base material, sterilizing, mixing the selenium-rich fertilizer into the soil base material, and regulating the pH value to obtain artificial soil; soaking oil peony seeds in gibberellin aqueous solution, washing and airing the soaked seeds, implanting the seeds into artificial soil, and spraying nutrient solution on the soil surface; spraying a foliar selenium-rich solvent periodically, wherein the foliar selenium-rich solvent is prepared from fluoroalkyl polyether modified polysiloxane, sodium selenite and water; topdressing, pest control and peony seed picking. According to the application, the artificial soil prepared by microbial fermentation is used for planting and producing the oil peonies, and the nutrient solution and the selenium-rich solvent are used in combination, so that the cultivated oil peonies have high seed setting rate and high selenium content in the peonies seeds, the economic value of the oil peonies is greatly improved, and the cultivation operation is simpler.

Description

Production method of peony for selenium-enriched oil
Technical Field
The application relates to the technical field of agricultural planting, in particular to a production method of peony for selenium-enriched oil.
Background
Selenium is an indispensable trace element of human body, is an antioxidant with strong capability, can effectively remove cancerogenic factors, namely free radicals, and has the reputation of resisting cancer. Scientists have investigated the relationship between cancer mortality in 20 countries and selenium intake in each individual and have found that the intake of selenium is small and the incidence and mortality of cancer are increased, with colon cancer, pancreatic cancer, ovarian cancer, prostate cancer, lung cancer and skin cancer being particularly pronounced in men. According to statistics, 42 countries and regions worldwide lack selenium; one investigation by the China's nutrition society shows that about 7 hundred million people in two-thirds of China are in a selenium-deficient state. The Chinese nutrition society recommends that the daily selenium intake of people is 50-200 micrograms, but the daily selenium intake of adult people in China is only 26-32 micrograms, and the situation of selenium malnutrition is ubiquitous in China.
In order to enable people to take a proper amount of selenium every day, the most effective way is to produce selenium-rich edible oil, and the selenium intake is improved through daily diet. While there are a variety of crops for producing edible oils, such as soybean, peanut, rapeseed, peony seed, etc. The peony seeds are obtained by cultivating the oil-used peony, the oil-used peony is used as an emerging woody oil crop, and the oil-used peony has the characteristics of large seed yield, high oil content, excellent quality, strong resistance and the like, can be planted on marginal lands, can intercropped with economic forests and the like, can be harvested continuously for more than 30 years in one year, and is worthy of being widely popularized in agricultural production. However, aiming at the production of peony for selenium-enriched oil, the existing production mode is complex, the seed setting rate is not high, the selenium content in peony seeds is less, and improvement is needed.
Disclosure of Invention
The application aims to provide a production method of peony for selenium-enriched oil, which solves the defects of complex operation, low seed setting rate, low selenium content in peony seeds and the like of the existing production method of peony for selenium-enriched oil.
The application realizes the above purpose through the following technical scheme:
the production method of the peony for the selenium-rich oil comprises the following steps of
(1) Mixing sodium selenite water solution with faeces Bombycis, and adding 1.0X10 × 8 -3.0×10 9 After uniformly stirring cfu/g of composite bacteria, controlling the temperature at 40-60 ℃, and sealing and fermenting for 10-15d to obtain the selenium-enriched fertilizer;
(2) Selecting a soil base material, sterilizing, mixing the selenium-rich fertilizer into the soil base material, uniformly stirring, and regulating the pH value to 6.8-7.5 to obtain artificial soil;
(3) Soaking oil peony seeds in gibberellin aqueous solution, washing and airing the soaked seeds, implanting the seeds into artificial soil, and spraying nutrient solution on the soil surface;
(4) Topdressing, pest control and peony seed picking.
The improvement is that after the artificial soil is planted, after the plant grows to 8-10cm in height, the foliar selenium-rich solvent is sprayed every 20-25d until the peony flowers, and the plant is sealed for 1-1.5h by a film cover after each foliar selenium-rich solvent is sprayed, wherein the foliar selenium-rich solvent is prepared by mixing fluoroalkyl polyether modified polysiloxane, sodium selenite and water.
The further improvement is that the weight ratio of the fluoroalkyl polyether modified polysiloxane, sodium selenite and water in the foliar selenium-rich solvent is the fluoroalkyl polyether modified polysiloxane to sodium selenite to water=0.05-0.5:0.4-0.8:120-150.
A further improvement is that the sodium selenite aqueous solution with the concentration of 50-300mg/L is mixed into the silkworm excrement according to the proportion of 0.002-0.01L/kg.
The further improvement is that the proportion of the selenium-enriched fertilizer mixed into the soil base material is 150-500g/kg.
The further improvement is that the concentration of the gibberellin aqueous solution is 150-300mg/L, and the soaking time is 24-48h.
The soil base material is further improved in that the soil base material comprises wood dust, wheat bran, sucrose and calcium sulfate, wherein the weight ratio of the wood dust to the wheat bran to the sucrose to the calcium sulfate=10-40:5-20:1-8:0.05-0.5.
The further improvement is that the composite bacteria are prepared by mixing bacillus beleiensis and stenotrophomonas maltophilia, and the ratio of the number of viable bacteria is bacillus beleiensis to stenotrophomonas maltophilia=1:5-5:1.
The further improvement is that the nutrient solution is formed by mixing one of rice soup or expired milk with magnesium acetate according to the weight ratio of 80-120:1.
The artificial soil is further improved in that the artificial soil is mixed with a water-retaining agent before being used, and the water-retaining agent is one of a polyacrylate water-retaining agent or a polyvinyl alcohol water-retaining agent.
In the application, silkworm excrement is selected as an organic fertilizer base material, and is firstly mixed with inorganic selenium source sodium selenite, and then composite bacteria are added for fermentation to prepare the selenium-rich fertilizer, which can improve the soil nutrient index and enhance the soil enzyme activity; the compound bacteria are prepared by mixing bacillus belicus and stenotrophomonas maltophilia, and can improve the absorption and bioconversion efficiency of plants on sodium selenite through microbial fermentation; compared with natural soil, the selenium-rich fertilizer is more abundant in nutrient content, free of harmful impurities and good in porosity, is beneficial to nutrient absorption of root systems of oil peonies, promotes growth and development, blooms and seeds, and can reduce loss of selenium content. After the seeds are planted, nutrient solution is sprayed on the soil surface, so that the nutrition supply of the seed germination stage is further ensured, a special foliar selenium-rich solvent is sprayed periodically, and fluoroalkyl polyether modified polysiloxane is added into the foliar selenium-rich solvent, so that the surface tension of a solvent system can be reduced, the leveling is promoted, the adhesive force and the moisture retention of the solvent are improved, the foliar selenium-rich solvent is not easy to volatilize and run off due to the cooperation of a film cover sealing measure, the foliar absorption effect is improved, the completion of the bioconversion of inorganic selenium is promoted, and the selenium content of peony seeds is increased.
The application has the beneficial effects that: according to the application, the artificial soil prepared by microbial fermentation is used for planting and producing the oil peonies, and the nutrient solution and the selenium-rich solvent are used in combination, so that the cultivated oil peonies have high seed setting rate and high selenium content in the peonies seeds, the economic value of the oil peonies is greatly improved, and the cultivation operation is simpler.
Detailed Description
The application will now be described in further detail with reference to the following examples, it being necessary to note that the following detailed description is given for the purpose of illustration only and is not to be construed as limiting the scope of the application, as numerous insubstantial modifications and adaptations of the application are possible in light of the above disclosure by those skilled in the art.
Example 1
The production method of the peony for the selenium-rich oil comprises the following steps of
(1) Mixing 300mg/L sodium selenite aqueous solution with faeces Bombycis at a ratio of 0.002L/kg, and adding 1.0X10 8 cfu/g of composite bacteria, wherein the composite bacteria are prepared by mixing bacillus beleiensis and stenotrophomonas maltophilia, the number ratio of viable bacteria is bacillus beleiensis to stenotrophomonas maltophilia=1:5, after uniform stirring, the temperature is controlled at 40 ℃, and the sealed fermentation is carried out for 15d, so that the selenium-enriched fertilizer is obtained;
(2) Selecting a soil base material, wherein the soil base material comprises wood dust, wheat bran, sucrose and calcium sulfate, the weight ratio of the components is that the wood dust comprises the wheat bran, the sucrose and the calcium sulfate=10:5:1:0.05, sterilizing, mixing the selenium-enriched fertilizer into the soil base material according to the proportion of 150g/kg, uniformly stirring, and regulating the pH value to 6.8 to obtain artificial soil;
(3) Soaking oil peony seeds in 150mg/L gibberellin water solution for 48 hours, washing and airing the soaked seeds, implanting the seeds into artificial soil, spraying nutrient solution on the soil surface, and mixing rice soup and magnesium acetate according to the weight ratio of 80:1;
(4) Topdressing, pest control and peony seed picking.
Example 2
The production method of the peony for the selenium-rich oil comprises the following steps of
(1) Mixing 300mg/L sodium selenite aqueous solution with faeces Bombycis at a ratio of 0.002L/kg, and adding 1.0X10 8 cfu/g of composite bacteria, wherein the composite bacteria are prepared by mixing bacillus beleiensis and stenotrophomonas maltophilia, the number ratio of viable bacteria is bacillus beleiensis to stenotrophomonas maltophilia=1:5, after uniform stirring, the temperature is controlled at 40 ℃, and the sealed fermentation is carried out for 15d, so that the selenium-enriched fertilizer is obtained;
(2) Selecting a soil base material, wherein the soil base material comprises wood dust, wheat bran, sucrose and calcium sulfate, the weight ratio of the components is that the wood dust comprises the wheat bran, the sucrose and the calcium sulfate=10:5:1:0.05, sterilizing, mixing the selenium-enriched fertilizer into the soil base material according to the proportion of 150g/kg, uniformly stirring, and regulating the pH value to 6.8 to obtain artificial soil;
(3) Soaking oil peony seeds in 150mg/L gibberellin water solution for 48 hours, washing and airing the soaked seeds, implanting the seeds into artificial soil, spraying nutrient solution on the soil surface, and mixing rice soup and magnesium acetate according to the weight ratio of 80:1;
(4) After the plant grows to 8cm in height, spraying a foliar selenium-rich solvent every 2d until the peony flowers, and sealing the plant for 1h by a film cover after each foliar selenium-rich solvent spraying, wherein the foliar selenium-rich solvent is prepared by mixing fluoroalkyl polyether modified polysiloxane, sodium selenite and water, and the weight ratio of the fluoroalkyl polyether modified polysiloxane, the sodium selenite and the water is fluoroalkyl polyether modified polysiloxane to sodium selenite to water=0.05:0.4:120;
(5) Topdressing, pest control and peony seed picking.
Example 3
The production method of the peony for the selenium-rich oil comprises the following steps of
(1) The concentration was adjusted to 0.007L/kg150mg/L sodium selenite aqueous solution is mixed into faeces Bombycis, and 1.0X10 is added 9 cfu/g of composite bacteria, wherein the composite bacteria are prepared by mixing bacillus beleiensis and stenotrophomonas maltophilia, the number ratio of viable bacteria is bacillus beleiensis and stenotrophomonas maltophilia=1:1, after uniform stirring, the temperature is controlled at 50 ℃, and the sealed fermentation is carried out for 12 days, so that the selenium-enriched fertilizer is obtained;
(2) Selecting a soil base material, wherein the soil base material comprises wood dust, wheat bran, sucrose and calcium sulfate, the weight ratio of the components is that the wood dust comprises the wheat bran, the sucrose and the calcium sulfate=20:12:5:0.2, sterilizing, mixing the selenium-enriched fertilizer into the soil base material according to the proportion of 400g/kg, uniformly stirring, and regulating the pH value to 7.2 to obtain artificial soil;
(3) Soaking oil peony seeds in 220mg/L gibberellin water solution for 36h, washing and airing the soaked seeds, implanting the seeds into artificial soil, spraying nutrient solution on the soil surface, and mixing expired milk and magnesium acetate according to the weight ratio of 100:1;
(4) After the plant grows to 9cm in height, spraying a foliar selenium-rich solvent every 3d until the peony flowers, and sealing the plant for 1.2h by a film cover after each foliar selenium-rich solvent spraying, wherein the foliar selenium-rich solvent is prepared by mixing fluoroalkyl polyether modified polysiloxane, sodium selenite and water, and the weight ratio of the fluoroalkyl polyether modified polysiloxane to the sodium selenite to the water=0.3:0.6:135;
(5) Topdressing, pest control and peony seed picking.
Example 4
The production method of the peony for the selenium-rich oil comprises the following steps of
(1) Mixing sodium selenite water solution with concentration of 50mg/L with faeces Bombycis at a ratio of 0.01L/kg, and adding 3.0X10 9 cfu/g of composite bacteria, wherein the composite bacteria are prepared by mixing bacillus beleiensis and stenotrophomonas maltophilia, the number proportion of viable bacteria is bacillus beleiensis, stenotrophomonas maltophilia=5:1, after uniform stirring, the temperature is controlled at 60 ℃, and the sealed fermentation is carried out for 10 days, so that the selenium-enriched fertilizer is obtained;
(2) Selecting a soil base material, wherein the soil base material comprises wood dust, wheat bran, sucrose and calcium sulfate, the weight ratio of the components is that the wood dust comprises the wheat bran, the sucrose and the calcium sulfate=40:20:8:0.5, sterilizing, mixing the selenium-enriched fertilizer into the soil base material according to the proportion of 500g/kg, uniformly stirring, and regulating the pH value to 7.5 to obtain artificial soil;
(3) Soaking oil peony seeds in 300mg/L gibberellin water solution for 48 hours, washing and airing the soaked seeds, implanting the seeds into artificial soil, spraying nutrient solution on the soil surface, and mixing the nutrient solution with magnesium acetate in the expired milk according to the weight ratio of 120:1;
(4) After the plant grows to 10cm in height, spraying a foliar selenium-rich solvent every 4d until the peony flowers, and sealing the plant for 1.5h by a film cover after each foliar selenium-rich solvent spraying, wherein the foliar selenium-rich solvent is prepared by mixing fluoroalkyl polyether modified polysiloxane, sodium selenite and water, and the weight ratio of the fluoroalkyl polyether modified polysiloxane to the sodium selenite to the water=0.5:0.8:150;
(5) Topdressing, pest control and peony seed picking.
Example 5
Picking seeds of the peony cultivated in any one of the embodiments 1-3, cleaning, removing impurities, peeling, squeezing, refining, decolorizing, deodorizing, and preparing selenium-enriched peony seed oil.
Comparative example 1
The method is basically the same as the step of the example 2, and the only difference is that the selenium-enriched fertilizer is directly fermented without adding complex bacteria during preparation.
Comparative example 2
It is essentially the same as example 2, except that the foliage selenium-rich solvent is sprayed without sealing the plant with a film cover.
Comparative example 3
The procedure is substantially the same as in example 2, except that when the selenium-rich solvent is sprayed on the foliage, the solvent used is aqueous sodium selenite solution and the fluoroalkyl polyether modified polysiloxane is absent.
Comparative example 4
It is essentially the same as the procedure of example 2, the only difference being that no nutrient solution is sprayed.
Comparative example 5
Common oil commercially available peony seeds.
The oil peonies obtained by the cultivation in examples 1 to 4 and comparative examples 1 to 5 were tested for seed formation and selenium content, respectively. The seed setting rate detection mode comprises the following steps: 10 tree peony for oil of each experimental example are randomly extracted, and the total seed setting number is counted; selenium content was detected by atomic fluorescence spectrometry. The statistics of the detection results are shown in the following table 1:
table 1: seed number and selenium content statistics
From the table, the application can produce the peony for oil by artificial soil planting mixed with the compound bacteria fermented selenium-enriched fertilizer, and the seed setting rate and the selenium content of the peony seeds for oil are greatly improved by matching with the nutrient solution, the special foliar selenium-enriched solvent and the spraying mode.
The foregoing examples illustrate only a few embodiments of the application and are described in detail herein without thereby limiting the scope of the application. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the application, which are all within the scope of the application.

Claims (4)

1. A production method of peony for selenium-rich oil is characterized by comprising the following steps: the steps include
(1) Mixing sodium selenite water solution with concentration of 50-300mg/L into faeces Bombycis according to the ratio of 0.002-0.01L/kg,then 1.0X10 were added 8 -3.0×10 9 After uniformly stirring cfu/g of composite bacteria, controlling the temperature at 40-60 ℃, and performing sealed fermentation for 10-15d to obtain the selenium-enriched fertilizer, wherein the composite bacteria are prepared by mixing bacillus beleiensis and stenotrophomonas maltophilia, and the ratio of the number of viable bacteria is bacillus beleiensis to stenotrophomonas maltophilia=1:1;
(2) Selecting a soil base material, sterilizing, mixing the selenium-rich fertilizer into the soil base material, uniformly stirring, and regulating the pH value to 6.8-7.5 to obtain artificial soil; the soil base material comprises wood dust, wheat bran, sucrose and calcium sulfate, wherein the weight ratio of the wood dust to the wheat bran to the calcium sulfate=10-40:5-20:1-8:0.05-0.5;
(3) Soaking oil peony seeds in gibberellin aqueous solution, washing and airing the soaked seeds, implanting the seeds into artificial soil, and spraying nutrient solution on the soil surface, wherein the nutrient solution is prepared by mixing one of rice soup or expired milk with magnesium acetate according to the weight ratio of 80-120:1;
after artificial soil is planted, after the plant grows to 8-10cm in height, spraying a foliar selenium-rich solvent every 20-25d until the peony flowers, and sealing the plant by a film cover after each foliar selenium-rich solvent spraying, wherein the foliar selenium-rich solvent is prepared by mixing fluoroalkyl polyether modified polysiloxane, sodium selenite and water, and the weight ratio of the fluoroalkyl polyether modified polysiloxane to the sodium selenite is that water=0.05-0.5:0.4-0.8:120-150;
(4) Topdressing, pest control and peony seed picking.
2. The method for producing peony for selenium-enriched oil according to claim 1, wherein the method comprises the following steps: the proportion of the selenium-enriched fertilizer mixed into the soil base material is 150-500g/kg.
3. The method for producing peony for selenium-enriched oil according to claim 1, wherein the method comprises the following steps: the concentration of the gibberellin aqueous solution is 150-300mg/L, and the soaking time is 24-48h.
4. The method for producing peony for selenium-enriched oil according to claim 1, wherein the method comprises the following steps: the artificial soil is mixed with a water-retaining agent before being used, and the water-retaining agent is one of a polyacrylate water-retaining agent or a polyvinyl alcohol water-retaining agent.
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Citations (11)

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