CN106818434A - A kind of natural propagation method of Seeds of Dendrobium Candidum bed sowing - Google Patents

A kind of natural propagation method of Seeds of Dendrobium Candidum bed sowing Download PDF

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Publication number
CN106818434A
CN106818434A CN201611144714.5A CN201611144714A CN106818434A CN 106818434 A CN106818434 A CN 106818434A CN 201611144714 A CN201611144714 A CN 201611144714A CN 106818434 A CN106818434 A CN 106818434A
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CN
China
Prior art keywords
seed
seeds
bed
dendrobium candidum
propagation method
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CN201611144714.5A
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Chinese (zh)
Inventor
明兴加
谢守清
李博然
王昌华
伍淳操
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Chongqing Wonderful Biological Science And Technology Co Ltd
China Academy of Chinese Medical Sciences CACMS
Chongqing Academy of Chinese Materia Medica
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Chongqing Wonderful Biological Science And Technology Co Ltd
Chongqing Academy of Chinese Materia Medica
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Application filed by Chongqing Wonderful Biological Science And Technology Co Ltd, Chongqing Academy of Chinese Materia Medica filed Critical Chongqing Wonderful Biological Science And Technology Co Ltd
Priority to CN201611144714.5A priority Critical patent/CN106818434A/en
Publication of CN106818434A publication Critical patent/CN106818434A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics

Abstract

The invention provides a kind of natural propagation method of Seeds of Dendrobium Candidum bed sowing, its main technical content is:Then 3 April, one day prior to seeding, seed is prepared into seed stock liquid in the way of each capsule seed and 3L nutrient mix, and soak 24h, seed stock liquid is uniformly sprayed on bed surface;From after planting, the stage is entirely sprouted, appropriate humidity need to be kept, and avoid strong illumination, spray within every 7 days the nutrient solution of MS desaccharification formulas once.Deficiency of the present invention according to existing seedlings of Dendrobium officinale mode of reproduction, the big superiority condition of seed amount contained by stem of noble dendrobium fruit is given full play to, under the suitable environmental condition of artificial creation, seed is directly sowed on seedbed, seedling is cultivated, and reaches the purpose for reducing and breeding flow and reduce cultivation cost.

Description

A kind of natural propagation method of Seeds of Dendrobium Candidum bed sowing
Technical field
The present invention relates to a kind of Chinese medicine implantation methods, more specifically to a kind of Seeds of Dendrobium Candidum bed sowing Natural propagation method.
Technical background
Dendrobium candidum (Dendrobium officinale Kimura et Migo) also known as ribbed hedyotis herb, are China's tradition names Your Chinese medicine, has functions that reinforcing stomach reg fluid, nourishing Yin and clearing heat, moistens the lung and relieve the cough, retched for impairment of yin body fluid deficiency, dry polydipsia, deficiency of food, Abnormal heat, the mesh secretly illness such as not clear after being ill.Its processed goods Tiepi Fengdou is the more high-grade health products of in the market.Due to long-term excessively Collection, dendrobium candidum wild resource is subject to heavy damage, as the endangered plants laid special stress on protecting both at home and abroad.In recent years, with The introducing of plant tissue culture technology, and vast researcher and enterprise effort, the artificial breeding of seedlings of Dendrobium officinale is able to Break through, plantation industry has also reached certain scale.But at the same time, seedling tissue culture also have that environmental requirement is strict, program is complicated, The shortcomings of pollution rate is high, toxigenic capacity is high, is badly in need of exploring new seedling breeding mode, promotes the sound development of stem of noble dendrobium industry.
Seeds of Dendrobium Candidum fine powder shape, without endosperm, the Inner nutrition material needed for lacking sprouting, but each capsule institute It is more containing seed amount, it is the premise and key of its protection of resources and sustainable use using cultivating seeds seedling.
The content of the invention
To solve the above problems, the invention provides a kind of natural propagation method of Seeds of Dendrobium Candidum bed sowing, bag Include following steps:
1) 600-1000 meters of height above sea level of selection, more than 0 DEG C of annual minimum temperature, the region of humidity more than 60% is used as sowing ground;
2) building has sprinkling irrigation in sunshade net inside and outside 75%, greenhouse, and dome portions lee face sets the greenhouse of wound membrane windowing;
3) set up liftoff 50-70 centimetres and build built on stilts seedbed, and lay matrix;
4) when month last year 11-12, fruit was fully ripe, collecting fruit prepares seed;
5) 3-4 months then, one day prior to seeding, by seed in the way of each capsule seed and 3L nutrient mix system Made preparations for sowing sub- storing solution, and soaks 24h, and seed stock liquid is uniformly sprayed on bed surface;
6) from after planting, the stage is entirely sprouted, appropriate humidity need to be kept, and avoid strong illumination, sprayed MS and go within every 7 days The nutrient solution of sugar formula is once.
Step 3) it is described laying matrix be:It is first 2 months in sowing, fresh wood shavings and sawdust are collected, wood shavings are laid on down Layer 5-7 cm thicks, sawdust is laid on 2-3 centimetres of upper strata, is then irrigated with water, and being watered 30 jin of water by 1 jin of cow dung dilutes, by what is diluted Cow dung liquid is uniformly spread on bed surface.After a dry tack free once after, then pour permeable, afterwards according to every m2The amount for spraying 3L is applied MS nutrient solutions once, and unanimously keep bed surface moistening, are watered by about one-month period, and hardened degree occurs in matrix.
Step 5) described in nutrient solution be with thin rice gruel be solution addition 1/2MS, NAA0.3mg/L, gibberellin 1.5mg/L.
The method have the benefit that:Deficiency according to existing seedlings of Dendrobium officinale mode of reproduction of the invention, fully The big superiority condition of seed amount contained by stem of noble dendrobium fruit is played, under the suitable environmental condition of artificial creation, seed is directly broadcast In on seedbed, seedling is cultivated, and reach to reduce and breed flow and reduce the purpose for cultivating cost.
Specific embodiment
Embodiment 1
1. seed collection and preservation
When month last year 11-12, fruit was fully ripe, collecting fruit.Now, part capsule natural cracking, seed is in light Yellow, dust-like.After collecting fruit, fruit one end is cut off with scissors, seed is scattered, room ventilation is collected and be stored in newspaper Place, it is standby.
2. the preparation in place is bred
The natural environmental condition of the suitable Growth of Dendrobium candidum of selection, height above sea level 600-1000 meters, more than 0 DEG C of annual minimum temperature, Humidity more than 60%, light application time is long, and water resource is enriched in region, can meet irrigation needs.
Greenhouse construction is circular arc vault stalk formula, and canopy wide 8 meters rises 4.8 meters, and it is bone that greenhouse uses light-duty galvanizing section bar Frame, gravity-flow ventilation form.Set with sprinkling irrigation, every greenhouse hogging top lee face in sunshade net, greenhouse inside and outside plastic foil, 75% Wound membrane opens a window, 1 road of side wall wound membrane windowing.
Liftoff 50-70 centimetres is built built on stilts seedbed, and bedside 1-1.4 meters, bed length is less than 40 meters, with plastics plain net, plank Deng poromeric material as base plate.
3. matrix prepares
Fresh wood shavings and sawdust are collected in first 2 months i.e. January December to next year of sowing, wood shavings are laid on 5-7 lis of lower floor Rice is thick, and sawdust is laid on 2-3 centimetres of upper strata, is then irrigated with water.Meanwhile, it is watered 30 jin of water by 1 jin of cow dung and dilutes, the ox that will be diluted Liquid manure body is uniformly spread on bed surface.After a dry tack free once after, then pour permeable, afterwards, spray the nutrient solution of MS desaccharification formulas Once, bed surface moistening and is unanimously kept.Through watering after a while, matrix has certain hardened degree, and seed is big when can prevent from sowing Amount is lost in.
4. sow
The preparation of 4.1 nutrient solutions:With thin rice gruel as solution, 1/2MS, NAA0.3mg/L and gibberellin 1.5mg/L are added, prepared Obtain nutrient solution A;With thin rice gruel as solution, 1/2MS, IAA0.5mg/L are added, prepare nutrient solution B;With water as solution, addition MS, prepares nutrient solution C.Shown in the laboratory milk table 1 of MS nutrient solutions.
Table 1MS cultivates formula of liquid
Annotation:The consumption of any of the above nutritional ingredient, except mother liquor I be 20 times of concentrates in addition to, remaining be 200 times it is dense Contracting liquid.
The germination induction of 4.2 seeds
In month next year 3-4, sow the previous day.The ratio that seed and 3L nutrient solutions mix is obtained according to 1 capsule of each stem of noble dendrobium Example prepares seed stock liquid.Nutrient solution A, B, C group add seed, respectively obtain seed stock liquid A, seed stock liquid B and seed Storing solution C.Seed stock liquid is sprayed on bed surface with sprayer, every kind of storing solution spray area is 30m2As one Experimental group.From after planting, in the case where humidity is suitable, greening by about 20 days seeds, but the cycle of germination and growth is more long, this If period matrix is dried spherula can be caused dead.Therefore, the stage is entirely sprouted, appropriate humidity need to be kept, and avoid high light Irradiation.After 30d is sowed, statistics each group sowing germination percentage.It is specific as shown in table 2.
The influence that the Different Nutrition liquid of table 2 germinates to Seeds of Dendrobium Candidum
Nutrient solution group
Nutrient solution A groups 4032±242
Nutrient solution B groups 76±13
Nutrient solution C groups 191±17
5 intertillage managements
Stem of noble dendrobium seed is tiny, lacks nutrition, in the stage of sprouting, needs appropriate spray nutritious liquor.After planting, MS is sprayed within every 7 days Sweeten off the nutrient solution of formula once.Until after seedling takes root, can suitably reduce the number of times for spraying.Because seed is tiny, growth Seedling grows uneven unavoidably.After largely seedling is observed, because the germination percentage of nutrient solution A groups is high, it is therefore desirable in time Thinning.Sowing later six months, the survival rate of statistical disposition group and average biomass of individual tree.Specifically it is shown in Table 3.
Treatment group Average biomass of individual tree (g)
Nutrient solution A groups 131±21 142.43
Nutrient solution B groups 27±5 109.59
Nutrient solution C groups 82±17 79.27

Claims (3)

1. a kind of natural propagation method that Seeds of Dendrobium Candidum bed is sowed, it is characterised in that:Comprise the following steps:
1)600-1000 meters of height above sea level of selection, more than 0 DEG C of annual minimum temperature, the region of humidity more than 60% is used as sowing ground;
2)Construction has sprinkling irrigation in sunshade net inside and outside 75%, greenhouse, and dome portions lee face sets the greenhouse of wound membrane windowing;
3)Set up liftoff 50-70 centimetres and build built on stilts seedbed, and lay matrix;
4)When month last year 11-12, fruit was fully ripe, collecting fruit prepares seed;
5)Then the 3-4 months, one day prior to seeding, seed is prepared into kind in the way of each capsule seed and 3L nutrient mix Sub- storing solution, and 24h is soaked, seed stock liquid is uniformly sprayed on bed surface;
6) from after planting, the stage is entirely sprouted, appropriate humidity need to be kept, and avoid strong illumination, sprayed within every 7 days MS desaccharifications and match somebody with somebody The nutrient solution of side is once.
2. the natural propagation method that Seeds of Dendrobium Candidum bed according to claim 1 is sowed, it is characterised in that:Step 3) It is described laying matrix be:It is first 2 months in sowing, fresh wood shavings and sawdust are collected, wood shavings are laid on lower floor's 5-7 cm thicks, saw End is laid on 2-3 centimetres of upper strata, is then irrigated with water, and being watered 30 jin of water by 1 jin of cow dung is diluted, and the cow dung liquid of dilution is uniformly spread In on bed surface;
After a dry tack free once after, then pour permeable, afterwards according to every m2The amount for spraying 3L applies MS nutrient solutions once, and unanimously Bed surface moistening is kept, is watered by about one-month period, hardened degree occurs in matrix.
3. the natural propagation method that Seeds of Dendrobium Candidum bed according to claim 1 is sowed, it is characterised in that:Step 5) Described nutrient solution be with thin rice gruel be solution addition 1/2MS, NAA0.3mg/L, gibberellin 1.5mg/L.
CN201611144714.5A 2016-12-13 2016-12-13 A kind of natural propagation method of Seeds of Dendrobium Candidum bed sowing Pending CN106818434A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111616038A (en) * 2020-05-20 2020-09-04 龙陵县石斛研究所 Dendrobium devonianum seedling growing method
CN112450058A (en) * 2020-11-11 2021-03-09 铜陵凤丹种植科技开发有限责任公司 Production method of peony for selenium-rich oil
CN115005073A (en) * 2022-06-22 2022-09-06 中国热带农业科学院热带作物品种资源研究所 Dendrobium autumn open seeding method based on napkin

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Cited By (5)

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Publication number Priority date Publication date Assignee Title
CN111616038A (en) * 2020-05-20 2020-09-04 龙陵县石斛研究所 Dendrobium devonianum seedling growing method
CN112450058A (en) * 2020-11-11 2021-03-09 铜陵凤丹种植科技开发有限责任公司 Production method of peony for selenium-rich oil
CN112450058B (en) * 2020-11-11 2023-09-12 铜陵凤丹种植科技开发有限责任公司 Production method of peony for selenium-enriched oil
CN115005073A (en) * 2022-06-22 2022-09-06 中国热带农业科学院热带作物品种资源研究所 Dendrobium autumn open seeding method based on napkin
CN115005073B (en) * 2022-06-22 2024-01-09 中国热带农业科学院热带作物品种资源研究所 Open autumn dendrobium stem sowing method based on dining paper towel

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