CN101606468B - Simple method for cultivating nostoc commune and cultivating device - Google Patents
Simple method for cultivating nostoc commune and cultivating device Download PDFInfo
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- CN101606468B CN101606468B CN2009101008512A CN200910100851A CN101606468B CN 101606468 B CN101606468 B CN 101606468B CN 2009101008512 A CN2009101008512 A CN 2009101008512A CN 200910100851 A CN200910100851 A CN 200910100851A CN 101606468 B CN101606468 B CN 101606468B
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Abstract
The invention discloses a simple method for cultivating nostoc commune and a cultivating device. A plastic pipe is enclosed into a frame; the frame is provided with a simple cultivating device of a cultivating net with hole diameter being 0.5-3.0mm; and nutrient solution with simple prescription is used for preparing an artificial water body nostoc commune seedlings cultivating mode with each-level standard control condition and simple facility, and a natural water body nostoc commune cultivating mode with large-scale, open and large-area and simple facility, wherein the artificial water nostoc commune cultivating mode comprises a first-stage cultivation with a flask, a second-stage cultivation with a plastic bucket and a nostoc commune cultivation with a cement pool. The cultivating device is simple, convenient, economic and practical, and has the advantages of no soil amelioration, recycle and floating bed type. The cultivating method has the advantages of simple facility, standard cultivating condition and low cultivating cost, and is suitable for cultivating the nostoc commune in the natural water body with lager area.
Description
Technical field
The present invention relates to the cultural method of Nostoc commune, be specifically related to a kind of simple and easy cultural method and culture apparatus of Nostoc commune.
Background technology
Nostoc commune Nostoc commune is the combination of fungi and algae, and the algae of its symbiosis is the beads algae, is common fixed nitrogen blue-green algae.Belong to Cyanophyta, nostocaceae.Furvous has the woodear of point image maceration, so name.The modes of reproduction of Nostoc commune is by heterocyst algal filament to be divided into several hormocystangium, and very easily from the heterocyst dialysis, each hormocystangium grows up to long filamentous by cell division again.Generation heterospore and chlamydospore nourish and generate on the filamentous.Nostoc commune is widely distributed, is born in the wetland surface more, and is the most common on the wetland behind the summer rain.Mineral matters such as rich in proteins, multivitamin and phosphorus, zinc, calcium are discovered that dish contained a kind of composition in land for building can suppress the activity of the acetylcholinesterase in people's brain, thereby can be produced curative effect to senile dementia.As cold food, the heat that can disappear, convergence, beneficial gas, make eye bright, cure mainly nyctotyphlosis, prolapse of the anus; External application can be controlled burn, scald and cosmetology etc.
Chinese invention patent number is 03119091.X, name is called a kind of method of cultivating Nostoc commune, this method discloses the Nostoc commune alcohol solution dipping, washs in sterile medium, puts into homogenizer, grind to form slurry, be inoculated into then in the culture fluid, temperature is controlled at 10-40 ℃, and light intensity is controlled at 10-1800 μ Em-2s-1, changed culture fluid in every 10-20 days, every cubic metre of culture fluid can be gathered in the crops the bright Nostoc commune 40kg that diameter is 3-7mm in every month.The cultural method of Nostoc commune all concentrates on to cultivate in vessel or the artificial culture pond and carries out at present, needs corresponding vessel or artificial culture pond, and culture fluid needs special prescription, and needs often to change culture fluid, and it is bigger to cultivate cost, also is unsuitable for large tracts of land and cultivates.
Summary of the invention
It is simple that technical problem to be solved by this invention provides a kind of cultivation facility, the condition of culture standard, it is lower to cultivate cost, is suitable for the simple and easy cultural method with the Nostoc commune of natural water large tracts of land cultivation, has characteristics such as not having soilization, circulationization utilization, floating bed formula; The present invention also provides the culture apparatus that is suitable for this method.
The present invention solves the problems of the technologies described above the technical scheme that is adopted: a kind of simple and easy cultural method of Nostoc commune is characterized in that comprising the steps:
A culture fluid configuration: after fresh water boils and filters, nitrogen and phosphorous nutrient joined in the fresh water after the filtration be mixed with culture fluid, contain quality in the described culture fluid of every 100ml and be 0.5～2.09 nitrogen and 0.2～0.89 phosphorus, make that nitrogen phosphorus mass ratio is 0.625～10: 1 in the described culture fluid; Nitrogen and phosphorous nutrient can be obtained by commercially available, also can be by nitrogenous fertilizer and phosphate fertilizer autogamy, and the quality of nitrogen is with 1.0g in the 100ml culture fluid, and the quality of phosphorus is better with 0.59.
The b one-level is cultivated: add the described culture fluid of step a in flask, insert Nostoc commune algae kind with the ratio of inoculating 0.2～1.0g germplasm in the described culture fluid of every 100ml again, it is 15 ℃～25 ℃ in temperature then, intensity of illumination is 1500～3000Lx, light application time is to cultivate under 10～14 hours the condition, be cultured to that the cell density of Nostoc commune reaches 5～100,000/ml in the culture fluid, obtain one-level Nostoc commune culture;
The c secondary is cultivated: above-mentioned one-level Nostoc commune culture is moved in the Plastic Drum, ratio in the volume ratio 1: 10～30 of one-level Nostoc commune culture and culture fluid adds the described culture fluid of step a to Plastic Drum, it is 20 ℃～25 ℃ in temperature then, intensity of illumination is 3000～6000Lx, light application time is for being no less than 12 hours, cultivate under the inflation culture condition, be cultured to the adhesion of algae kind and become little strip, obtain secondary Nostoc commune culture;
D Nostoc commune seedling is cultivated: above-mentioned secondary Nostoc commune culture is moved in the cement pit, ratio in the volume ratio 1: 8～30 of secondary Nostoc commune culture and culture fluid adds the described culture fluid of step a to cement pit, liquid level in the described cement pit is at 0.6～1.5m, liquid pH value is 7.0～9.2, it is 20 ℃～25 ℃ in temperature then, natural lighting and daytime inflate under the culture condition and cultivate, and cultivate 7～20 days, obtain adhesion and become block Nostoc commune seedling;
The e Nostoc commune is cultivated: pull above-mentioned Nostoc commune seedling out with 10～60 purpose bolting silk nets, the cultivation that described Nostoc commune seedling is positioned over the culture apparatus that floats over the nature water surface is cultivated on the net, every cultivation allowed described Nostoc commune seedling do the nature water surface 2～5 hours in 1～4 day, was cultured to Nostoc commune and gathered.Also can cultivate on the net with gathering the cultivation that wild Nostoc commune seedling is put in the culture apparatus of the nature water surface.
Cultivate in Plastic Drum, placing gas exhausting stone and/or being connected with the fan blower inflation in inflation described in the step c.
Daytime described in steps d inflation cultivate in cement pit per 1～4 square metre place a gas exhausting stone and/or be connected with the fan blower inflation.
Every square metre of described Nostoc commune seedling 200～400 grams of the online placement of described cultivation in step e; Can reach culture density preferably.
The culture apparatus of above-mentioned simple and easy cultural method comprises the framework that surrounds with plastic tube, and it is 0.5～3.0 millimeter cultivation net that described framework is provided with the mesh aperture.This culture apparatus is simple and easy, convenient, economical and practical, 0.5～3.0 millimeters cultivation net has prevented that other zooplanktons from entering this cultivation net, has also prevented Nostoc commune floating going out from mesh, when more helping doing, in time drain free water and the tamper of removing on the mesh.Framework can be polygon, garden shape and ellipse etc.Plastic tube also can be used replacements such as bamboo trunk, timber, cultivates net and can use making such as gauze, nylon and vinylon.
The top of described framework both sides is symmetrically arranged with pulley, and described pulley is provided with suspension rope, and described suspension rope is connected with described framework.Use the pulley sus-pension, do when promptly having made things convenient for Nostoc commune to cultivate, also help Nostoc commune and gather.Pulley can be used making such as wood, plastics or metal.
Compared with prior art, the invention has the advantages that culture fluid configuration by filling a prescription simple and clear, controlled condition standards at different levels, the easy artificial water Nostoc commune of facility seedling training method, scale, open and large-area natural water, the easy Nostoc commune training method of facility, the Nostoc commune seedling cultivation of artificial water comprise with the flask one-level cultivates, cultivates and cultivate with cement pit Nostoc commune seedling with the Plastic Drum secondary; We are by the habit of growth of research Nostoc commune; use the cultivation of simple facility and condition standard respectively by the habit of growth of the different physiological stages of Nostoc commune; help improving the Nostoc commune survival rate of young and shorten the seed incubation time; more help Nostoc commune and in natural water, carry out open, large tracts of land large-scale production; improved the growth rate of Nostoc commune; growth rate reaches 25%; biomass increased also fast when Nostoc commune was cultivated; can double in general 3～5 days, and make ordinary people also can carry out the large-scale production Nostoc commune.Because suitable doing reduced the time of contact with harmful organisms; The tamper that may occur on the mesh of cultivating net is in time removed, kept normally carrying out of Jovian satellites and water exchange; Particularly improved the quantity and the quality of Nostoc commune secretion gelatinous sheath, increased the content of soluble dietary fiber, strengthened the ability that opposing environment association compels, thereby prolonged the life-span of cultivating.The present device cost is lower, make easily, ordinary people can just can be used as the cultivation facility with standing material and independently cultivate, therefore the present invention is that a kind of cultivation facility is simple, the condition of culture standard, it is lower to cultivate cost, is suitable for the simple and easy cultural method with the Nostoc commune of natural water large tracts of land cultivation.
Description of drawings
Fig. 1 is this bright culture apparatus schematic diagram.
Embodiment
Embodiment describes in further detail the present invention below in conjunction with accompanying drawing.
The culture apparatus of a kind of Nostoc commune as shown in Figure 1, comprise the framework 3 that surrounds with plastic tube, it is 0.5～3.0 millimeter cultivation net 4 that framework 3 is provided with the mesh aperture, the top of framework 3 both sides is symmetrically arranged with pulley 1, and pulley 1 usefulness shelf is fixed, and pulley 1 is provided with suspension rope 2, suspension rope 2 is fixedlyed connected with framework 3, when the Nostoc commune of cultivating on the net 4 was done, as long as pull-up suspension rope 2 allows framework 3 and cultivation net 4 sling, fixedly suspension rope 2 just could again.Do end, put down suspension rope 2 and get final product, pull-up suspension rope 2 is also easily gathered with regard to the energy draining when adopting simultaneously.
The culture fluid preparation of simple and easy cultivation Nostoc commune: the quality with nitrogen in the 100ml culture fluid is 0.5～2.0g (better about 1.0g), the quality of phosphorus is that 0.2～0.8g (better about 0.5g) is standard, with commercially available nitrogenous-phosphatic manure with boil and filters after the cleaning mixing of fresh water, stirring is made into this culture fluid.Nitrogenous-phosphatic manure also can be used nitrogenous fertilizer and phosphate fertilizer autogamy.
A kind of simple and easy cultural method of Nostoc commune, in conical flask, add the culture fluid (culture fluid of the foregoing description 2, as follows) 2000ml, inoculating 4～20g Nostoc commune algae kind then goes in the conical flask, in temperature is 15 ℃～25 ℃, and intensity of illumination is 1500～3000Lx, and light application time is to cultivate under 10～14 hours the condition, the cell density that is cultured to Nostoc commune in the interior culture fluid of conical flask reaches 5～100,000/ml, obtains one-level Nostoc commune culture; Above-mentioned one-level Nostoc commune culture is moved in the Plastic Drum, add again and arrive about culture fluid 40000ml in the Plastic Drum (also can replace) with other bucket, bag, it is 20 ℃～25 ℃ in temperature then, intensity of illumination is 3000～6000Lx, light application time is for being no less than 12 hours (can 13,14,15,16,18 hours etc.), with cultivating under the gas exhausting stone inflation culture condition, be cultured to the adhesion of algae kind and become little strip, obtain secondary Nostoc commune culture; Above-mentioned secondary Nostoc commune culture is moved in the cement pit, add again about culture fluid 800000ml in cement pit, allow the interior liquid level of cement pit be controlled at 0.6～1.5m, pH value in the cement pit is 7.0～9.2, it is 20 ℃～25 ℃ in temperature then, natural lighting and inflate under the culture condition daytime with gas exhausting stone and fan blower and to cultivate was cultivated 7～20 days, obtained adhesion and became block Nostoc commune seedling; Pull above-mentioned Nostoc commune seedling out with 10～60 purpose bolting silk nets, with 200～400 the gram/square metre the placement amount, the cultivation that above-mentioned Nostoc commune seedling is positioned over the culture apparatus of the foregoing description 1 that floats over the nature water surface is cultivated on the net, 1～4 day interval of every cultivation, allow the Nostoc commune seedling do the nature water surface 2～5 hours, do by weather conditions and determine, general fine day length blanking time, the time of doing can lack, overcast and rainy Ji Tian just did with one day one, the time of doing can prolong, be cultured to Nostoc commune and gather, every square metre of every month bright Nostoc commune of 1000 grams of can gathering.
In the foregoing description 3 in Plastic Drum the volume ratio of the culture fluid of one-level Nostoc commune culture and interpolation also can be 1: 10,1～15,1～25 or 1～30 etc.The volume ratio of the culture fluid of secondary Nostoc commune culture and interpolation also can be 1: 8,1～15,1～25 or 1～30 etc. in cement pit.Do not enumerate one by one at this.
In the foregoing description 3, flask, Plastic Drum and cement pit should be sterilized in advance, courses of infection when preventing to cultivate.
If temporary transient some difficulty of the grasp of every controlled condition when the Nostoc commune seedling cultivated, also can directly gather wild Nostoc commune seedling as seedling replace culture fluid configuration, one-level to cultivate, secondary is cultivated and the Nostoc commune seedling is cultivated the culture apparatus natural water cultivation that is put in embodiment 1.
Claims (5)
1. the simple and easy cultural method of a Nostoc commune is characterized in that comprising the steps:
A culture fluid configuration: after fresh water boils and filters, nitrogen and phosphorous nutrient joined in the fresh water after the filtration be mixed with culture fluid, containing quality in the described culture fluid of every 100ml is the nitrogen of 0.5～2.0g and the phosphorus of 0.2～0.8g, makes that nitrogen phosphorus mass ratio is 0.625～10: 1 in the described culture fluid;
The b one-level is cultivated: add the described culture fluid of step a in flask, insert Nostoc commune algae kind with the ratio of inoculating 0.2～1.0g germplasm in the described culture fluid of every 100ml again, it is 15 ℃～25 ℃ in temperature then, intensity of illumination is 1500～3000Lx, light application time is to cultivate under 10～14 hours the condition, be cultured to that the cell density of Nostoc commune reaches 5～100,000/ml in the culture fluid, obtain one-level Nostoc commune culture;
The c secondary is cultivated: above-mentioned one-level Nostoc commune culture is moved in the Plastic Drum, add the described culture fluid of step a to Plastic Drum in the ratio of the volume ratio 1: 10～30 of one-level Nostoc commune culture and culture fluid, it is 20 ℃～25 ℃ in temperature then, intensity of illumination is 3000～6000Lx, light application time is for being no less than 12 hours, cultivate under the inflation culture condition, be cultured to the adhesion of algae kind and become little strip, obtain secondary Nostoc commune culture;
D Nostoc commune seedling is cultivated: above-mentioned secondary Nostoc commune culture is moved in the cement pit, add the described culture fluid of step a to cement pit in the ratio of the volume ratio 1: 8～30 of secondary Nostoc commune culture and culture fluid, liquid level in the described cement pit is at 0.6～1.5m, liquid pH value is 7.0～9.2, it is 20 ℃～25 ℃ in temperature then, natural lighting and daytime inflate under the culture condition and cultivate, and cultivate 7～20 days, obtain adhesion and become block Nostoc commune seedling;
The e Nostoc commune is cultivated: pull above-mentioned Nostoc commune seedling out with 10～60 purpose bolting silk nets, the cultivation that described Nostoc commune seedling is positioned over the culture apparatus that floats over the nature water surface is cultivated on the net, every cultivation allowed described Nostoc commune seedling do the nature water surface 2～5 hours in 1～4 day, being cultured to Nostoc commune gathers, described culture apparatus comprises the framework that surrounds with plastic tube, it is 0.5～3.0 millimeter cultivation net that described framework is provided with the mesh aperture, the top of described framework both sides is symmetrically arranged with pulley, described pulley is provided with suspension rope, described pulley is fixed with shelf, and described suspension rope is connected with described frame fixation.
2. the simple and easy cultural method of a kind of Nostoc commune as claimed in claim 1 is characterized in that cultivating to placing gas exhausting stone and/or be connected with the fan blower inflation in Plastic Drum in inflation described in the step c.
3. the simple and easy cultural method of a kind of Nostoc commune as claimed in claim 1, it is characterized in that inflation on daytime described in the steps d cultivate in cement pit per 1～4 square metre place a gas exhausting stone and/or be connected with the fan blower inflation.
4. the simple and easy cultural method of a kind of Nostoc commune as claimed in claim 1 is characterized in that every square metre of described Nostoc commune seedling 200～400 grams of the online placement of described cultivation in step e.
5. the culture apparatus that is used for the simple and easy cultural method of the described a kind of Nostoc commune of claim 1, it is characterized in that comprising the framework that surrounds with plastic tube, it is 0.5～3.0 millimeter cultivation net that described framework is provided with the mesh aperture, the top of described framework both sides is symmetrically arranged with pulley, described pulley is provided with suspension rope, described pulley is fixed with shelf, and described suspension rope is connected with described frame fixation.
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| CN2009101008512A CN101606468B (en) | 2009-07-16 | 2009-07-16 | Simple method for cultivating nostoc commune and cultivating device |
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| CN2009101008512A CN101606468B (en) | 2009-07-16 | 2009-07-16 | Simple method for cultivating nostoc commune and cultivating device |
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| CN101606468A CN101606468A (en) | 2009-12-23 |
| CN101606468B true CN101606468B (en) | 2011-01-05 |
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| CN102351580B (en) * | 2011-10-14 | 2013-01-09 | 福建农林大学 | Method for preparing black fungus nutrient solution by utilizing food processing residues |
| CN102630494B (en) * | 2012-04-25 | 2013-09-18 | 中国科学院武汉植物园 | Cultural method and device for common nostoc |
| CN104521567B (en) * | 2014-12-25 | 2017-02-01 | 新疆大学 | Method for isolated culture of artificial strains of Helvella leucopus Pers |
| CN111363683A (en) * | 2019-04-17 | 2020-07-03 | 张兰英 | Method and device for culturing edible tree fungi in indoor and outdoor artificial large-scale cultivation land |
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