CN108541514A - A method of cultivating orange newborn mushroom offspring - Google Patents
A method of cultivating orange newborn mushroom offspring Download PDFInfo
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- CN108541514A CN108541514A CN201810235925.2A CN201810235925A CN108541514A CN 108541514 A CN108541514 A CN 108541514A CN 201810235925 A CN201810235925 A CN 201810235925A CN 108541514 A CN108541514 A CN 108541514A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/64—Cultivation containers; Lids therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/021—Pots formed in one piece; Materials used therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0293—Seed or shoot receptacles
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Mycology (AREA)
- Mushroom Cultivation (AREA)
Abstract
A method of cultivating orange newborn mushroom offspring, it is characterised in that：Include the following steps：1）Prepare device for raising seedlings：2）Cultivating aseptic seedling；3）It is prepared by microbial inoculum；4）Inoculation；5）Seedling medium is added；6）Installation water guider and shading are kept off the rain dust cover；7）Mycorrhiza fungi seeding cultivating；Orange newborn mushroom offspring is cultivated using the device designed by this patent, miscellaneous bacteria infection rate is low, and Lasiosphaera fenzlii class infection rate is 0.
Description
Technical field
The invention belongs to botanical seedling culturing technical field more particularly to a kind of methods for cultivating orange newborn mushroom offspring.
Background technology
The edible mycorrhizal fungis such as bolete, ferfas, delicious lactarius are one kind important in edible mushroom, its symbiosis is in the root of trees
It fastens to form exotrophic mycorrhiza, it absorbs the photosynthate of plant, while the nutrient supply trees that it is absorbed from soil utilize,
It is symbiosis.Artificial cultivation mycorhiza edible mushroom common practice is that symbiosis tree is first cultivated in the culture substrate of sterilizing at present
Seedling is seeded to aseptic seedling root system, then by the mycorhiza after inoculation, in laboratory by microbial inoculum of brood bodies such as mycelium or spores
Or in open-air condition lower refining seedling after greenhouse culture for a period of time（Some not hardenings）, wait for that Va Mycorrhiza Seedling reaches requirement, after testing
It is transplanted to production site fruiting afterwards.However, main problem of the existing technology has：（1）Mycorrhiza fungi seeding cultivating can only be in temperature
It is cultivated in room greenhouse or laboratory, it is difficult to which large-scale production, production operation is not also strong, and facility input is big, and Va Mycorrhiza Seedling is produced into
This height.(2) the mycorrhiza fungi seeding cultivating period is long, needs a large amount of clean water, after mainly using high-temperature sterilization under laboratory condition at present
Tap water, cost is higher.Large-scale production uses tap water or rainwater, and there are the infection of non-purpose VA Mycorrhizal Fungi.(3) it connects at present
The common method of kind aseptic seedling has the inoculation of microbial inoculum root dipping, microbial inoculum to broadcast sowing inoculation and punching filling microbial inoculum inoculation.Microbial inoculum root dipping and bacterium
Agent broadcasts sowing inocalation method and is both needed to transplanting aseptic seedling, be easy to cause seedling root damage in transplanting seedling-slowing stage, encounters the not in due course seedling of weather
The wooden easily death leads to inoculation failure, although influencing little, inoculation on nursery stock using conventional punching filling microbial inoculum inocalation method
Microbial inoculum is in direct contact that chance is small with seedling root, and the growth speed of inoculation is very slow and is not easy long-term surviving, to reduce
Mycorhiza infection rate.(4) after aseptic seedling inoculation microbial inoculum, three months after being especially just inoculated with, the water management of culture substrate was
It is very difficult and be difficult to accurately to grasp, it often results in mycorhiza infection rate and reduces even nursery stock and decay and dead.(5) made at present
The container for cultivating Va Mycorrhiza Seedling is general container for plant growth, needs to be placed under greenhouse or indoor conditions and uses, needs daylight
Lamp provides light source, and energy consumption is big, of high cost, is unfavorable for large-scale cultivation Va Mycorrhiza Seedling.Illumination is insufficient under the conditions of greenhouse, affects seedling
The photosynthesis and mycorhiza synthetic effect of wood.(6) when the nursery stock of Arbuscular Mycorrhizal Fungi bacterium is directly placed at dew using common container for plant growth
When under the conditions of it, seedling medium is easy dehydration, the brood bodies such as non-purpose VA Mycorrhizal Fungi spore of nature（Can by rainwater, wind or
Underground soil is infected into matrix, to infect non-purpose VA Mycorrhizal Fungi（Such as Lasiosphaera fenzlii class, palpus false truffle）, easily pollute, lead
Cause the failure of purpose mycorrhiza fungi seeding cultivating.
Invention content
For the above-mentioned problems in the prior art, the object of the present invention is to provide one kind to be suitable for training under open-air condition
Educate the method for orange newborn mushroom offspring.By design specialized device, Va Mycorrhiza Seedling root system part is made to be in opposite in full growth course
Growth and development under sealing condition, the non-more difficult intrusion seedling root of purpose fungus breeding body infects nursery stock, and purpose VA Mycorrhizal Fungi passes through
Be easy to survive after artificial infection, so can with scale, low cost cultivate qualified Va Mycorrhiza Seedling.
To achieve the above object, present invention employs following technical solutions：
A method of cultivating orange newborn mushroom offspring, it is characterised in that：Include the following steps：
1）Prepare device for raising seedlings：The device includes vessel, and shading is kept off the rain dust cover and water guider；
The vessel side wall is equipped with air hole and inoculation hole, and vessel side wall is provided inwardly with platform with bottom surface junction
Rank is equipped with orifice plate on step, and the vessel side wall between bottom surface and orifice plate is equipped with osculum；
The shading keeps off the rain dust cover upper end equipped with elastic tying, and lower end is equipped with water outlet, and tied rope is equipped on elastic tying；
The shading dust cover that keeps off the rain is made using black and white reflecting film material, and black is inwardly outwardly for white；
The water guider is made of hollow bend pipe and the three foot guide supports for being secured to connect, the three feet guide support
Lower section be water guide groove；Inoculation port lid is equipped in the inoculation hole, inoculation port lid edge is equipped with to be consolidated for that will plant port lid
The inside and outside buckle being scheduled on vessel；
2）Cultivating aseptic seedling：Masson pine aseptic seedling is cultivated in vessel；
3）It is prepared by microbial inoculum：Prepare orange newborn mushroom liquid bacterial agent；
4）Inoculation：It is inoculated in month autumn and winter 10-11, inoculation method is：After masson pine aseptic seedling has lateral root development, inoculation is removed
Port lid enters 5-10cm punchings at the inoculation hole of vessel using card punch downward oblique cutting at inoculation hole, the access orange per hole
Color breast mushroom liquid bacterial agent 5-10ml；Inoculation port lid is fixed at inoculation hole by inside and outside buckle and is sealed, portion is to educating from container
Seedling matrix, which is uniformly poured, spreads nutrient solution about 300-500ml；
5）Seedling medium is added：Sterilized seedling medium is added above the seedling medium for spreading overnutrition liquid again until dress pouring
Full vessel, keeps stromal surface slightly at steamed bun shape；
6）Installation water guider and shading are kept off the rain dust cover：Water guider is put on the seedling medium slightly at steamed bun shape, it is desirable that
The stem of bend pipe and aseptic seedling reclines, and shading is then covered on vessel keeps off the rain dust cover, the rhizome and water guide of aseptic seedling
The bend pipe of device keeps off the rain the outside of dust cover across elastic tying and positioned at shading, and tied rope is fastened at elastic tying with tied rope；
7）Mycorrhiza fungi seeding cultivating：Water guider will be installed and the keep off the rain Va Mycorrhiza Seedling of device of dust cover of shading is placed on open-air condition
Lower culture；2 week did not watered after inoculation, and backsight water content of substrate situation pours moisture, kept water content of substrate in 60-
It is cultivated under the conditions of 70%；It pours water source and comes from Deep Underground water.
A kind of method of the orange newborn mushroom offspring of cultivation, it is characterised in that step 2）The training of middle masson pine aseptic seedling
Educating method is：Masson pine seed is taken, is drained away the water after fully being impregnated with tap water, seed inferior is removed, with 0.1% permanganic acid
Potassium impregnates 1 hour and carries out surface sterilization, is seeded in the vessel equipped with seedling medium, keeps after aseptic water washing 3 times
Water content of substrate 60-70% is cultivated in greenhouse or greenhouse；Water source is phreatic water；Seedling medium need to be in 121 DEG C, 0.1MPa
Sterilize 1h.
A kind of method of the orange newborn mushroom offspring of the cultivation, it is characterised in that：The seedling medium formula is vermiculite：
Sandy loam=1:1（Volume ratio）, natural pH value.
A kind of method of the orange newborn mushroom offspring of cultivation, it is characterised in that step 3）In orange newborn mushroom liquid bacterial agent
Preparation method is as follows：
1）Strain tissue detaches：Orange newborn massee fruiting bodies are acquired, laboratory is taken back and carries out tissue separation, cap is taken to be connected with stem
Position mung bean size tissue, the purifying culture of picking mycelia is spare after a week is cultivated on solid medium；
2）Strain expands numerous：Cultured strain is taken, strain block is taken to be put into shaking flask culture in fluid nutrient medium, 25 DEG C of cultivation temperature,
Rotating speed 140r/min cultivates 25d, spare；
3）It is prepared by liquid bacterial agent：Take step 2）Bacterium ball is obtained by filtration in middle bacterium solution, and fluid nutrient medium, bacterium ball and fluid nutrient medium is added
By volume 1:1 mixing；Then sodium alginate is added in mixed liquor, crushing, which is mixed thoroughly, is made liquid bacterial agent；The sodium alginate
The amount of 3-5g is added to be added by every 1L mixed liquors.
A kind of method of the orange newborn mushroom offspring of the cultivation, it is characterised in that：The Liquid Culture based formulas：Ma Ling
Potato 200g, which boils, filters to take juice, glucose 20g, brown sugar 5g, 1.5 g of potassium dihydrogen phosphate, 1 g of magnesium sulfate, 1 g of ammonium chloride, albumen
Peptone 1g, 1% ironic citrate 0.5ml, 0.1% zinc sulfate 1ml, vitamin B1 10mg add water to be settled to 1000ml；The solid training
Based formulas is supported to be made by every 1L additions agar 20g of aforesaid liquid culture medium.
A kind of method of the orange newborn mushroom offspring of the cultivation, it is characterised in that：Card punch described in step 4) by
It punches end and handle end two parts is constituted, the punching end is flat sharp contract type, a length of 5-6cm, width 1.5-1.8cm, hand
Pommel is cylinder, a length of 8-12cm, a diameter of 1.5-1.8cm.
A kind of method of the orange newborn mushroom offspring of the cultivation, it is characterised in that：The vessel bottom surface and hole
The distance between plate is 1-2cm, i.e., the height of step is 1-2cm.
A kind of method of the orange newborn mushroom offspring of the cultivation, it is characterised in that：The phreatic water is from depth
15m underground water below.
A kind of method of the orange newborn mushroom offspring of the cultivation, it is characterised in that：Connecing on the vessel side wall
Kind of hole is circle, bore dia 1.5-2cm, and inoculation hole is arranged 5, is uniformly distributed in a ring, be arranged in vessel on
Portion.
The present invention has the advantages that compared with prior art：（1）Apparatus structure designed by the present invention is simple, makes
With conveniently, exempts from the facilities such as greenhouse, non-purpose VA Mycorrhizal Fungi can be substantially reduced with large-scale cultivation purpose exotrophic mycorrhiza seedling（Such as Lasiosphaera fenzlii
Class, palpus false truffle etc.）Infection.（2）Vessel is the hard plastics of black non transparent, plays support frame, black
Material provides dark situation for VA Mycorrhizal Fungi and root system.The interior sky for having 1-2cm between vessel bottom surface and orifice plate, avoid with
Nursery lot is in direct contact, and prevents indigenous Va Mycorrhiza Seedling infection to seedling medium；Side wall, which is equipped with air hole, can meet VA Mycorrhizal Fungi
With root system respiration demand.Side wall facilitates punching and purpose microbial inoculum to be inoculated with equipped with inoculation hole.（3）Shading keep off the rain dust cover be it is soft
Black and white reflective plastic film, inwardly, upper end ties up to nursery stock rhizome to black to white there are osculum and using springy tied rope outwardly
Portion avoids infecing non-purpose VA Mycorrhizal Fungi through the media such as rainwater or wind, while illumination can be reflected by black and white reflective plastic film
Nursery stock leaf portion increases photosynthesis, at the same reduce the direct projection of matrix is influenced due to illumination moisture in matrix scatter and disappear and
Matrix medium temperature spends the high injury to VA Mycorrhizal Fungi and root system.（4）The inoculation method of the present invention is added using punching filling microbial inoculum
Nutrient solution induces microbial inoculum continued growth, extends the survival period of strain, can suitably cut off lateral root using sharp card punch or hurt
Cortical tissue is conducive to mycelia and infects root system, and the inoculation method is unaffected to seedling growth, while can greatly improve mycorhiza infection
Rate.(5) on the one hand the water guider designed by the present invention plays the role of watering to Va Mycorrhiza Seedling, on the other hand play support and hide
The bend pipe of the effect of light trap rain dust cover, water guider connects water pipe for drip irrigation, that is, uses the trickle irrigation of osmosis type to water, irrigate one
Secondary, the long period without pouring, preferably maintains the moisture content of seedling medium, keeps the stabilization of seedling root growing environment
Property, reduce because moisture changes the influence to VA Mycorrhizal Fungi and root system, three months after being especially just inoculated with.（6）Saving of the present invention
Use water, the keep off the rain use of dust cover of one side shading reduces the moisture evaporation of seedling medium, and on the other hand the present invention uses
Underground water supply solves the problems, such as to need with a large amount of sterile waters in production.（7）The more conventional container for plant growth of the present invention cultivates mycorhiza
Seedling, miscellaneous bacteria infection rate are substantially reduced, and purpose mycorhiza synthetic ratio greatly improves.The present invention can not have to greenhouse facility or indoor conditions is
Va Mycorrhiza Seedling can be cultivated under open-air condition, solve Va Mycorrhiza Seedling scale, low cost, high quality cultivate series technique ask
Topic, the container can also be used for arid area seedling nursery, can greatly save with water, raising plant percent.
Description of the drawings
Fig. 1 is the structural schematic diagram that the present invention cultivates Va Mycorrhiza Seedling；
Fig. 2 present invention cultivates the schematic cross-sectional view of Va Mycorrhiza Seedling；
Fig. 3 be do not load onto shading keep off the rain dust cover when the present invention structural schematic diagram；
Fig. 4 is the cross-sectional view of vessel in the present invention；
Fig. 5 is the structural schematic diagram of water guider in the present invention；
Fig. 6 is present invention inoculation port lid structural schematic diagram；
Fig. 7 is the structural schematic diagram of card punch in the present invention；
Fig. 8 is card punch three-view diagram in the present invention；
Fig. 9 is orange newborn mushroom-Mycorrhizal Fungi of Pinus massoniana aspect graph
In figure：1- vessels；2- shadings are kept off the rain dust cover；3- water guiders；1-1- air holes；1-2- inoculation holes；1-3- platforms
Rank；1-4- osculums；1-5- orifice plates；1-6- is inoculated with port lid；The inside and outside buckles of 1-7-；The elastic tyings of 2-1；2-2- water outlets；2-3-
Tied rope；3-1- bend pipes；Tri- foot guide supports of 3-2-；3-3- water guide grooves；4-1- punches end；4-2- handle ends.
Specific implementation mode
1-9 and specific implementation mode are described in further details the present invention with reference to the accompanying drawings of the specification.
Embodiment 1
A kind of device suitable for mycorrhiza fungi seeding cultivating under open-air condition designed by the present invention, is mainly made of following three parts：
Vessel 1 for cultivating aseptic seedling and Va Mycorrhiza Seedling, the shading for covering vessel are kept off the rain dust cover 2, and are used for
To the water guider 3 of Va Mycorrhiza Seedling water filling.
Vessel 1 designed by the present invention is shown in Fig. 4, and air hole 1-1 is arranged on 1 side wall of vessel, realizes nursery
The better gas permeability of matrix root system of plant, meets VA Mycorrhizal Fungi and root system respiration demand；It is additionally provided with inoculation on 1 side wall of vessel
Hole 1-2 facilitates punching and purpose microbial inoculum to be inoculated with.In order to avoid container for plant growth bottom is in direct contact with nursery lot, indigenous mycorhiza is avoided
To seedling medium, the present invention is provided inwardly with step 1-3 in 1 side wall of vessel and bottom surface junction, matches on step for seedling infection
Setting orifice plate 1-5 is closed, the vessel side wall between bottom surface and orifice plate is equipped with osculum 1-4, between bottom surface and orifice plate
The interior sky of 1-2cm is kept, i.e. the height of step 1-3 is 1-2cm, in use, seedling medium is located on orifice plate 1-5, in matrix
Extra moisture is flowed out between bottom surface and orifice plate in the air, then through the osculum on side wall by the hole on orifice plate 1-5
1-4 flows out vessel.Inoculation hole 1-3 on vessel side wall may be configured as circle, bore dia 1.5-2cm, and inoculation hole is set
3-6 are set, most preferably 5, is uniformly distributed, is arranged in the middle and upper part of vessel in a ring.In order to facilitate inoculation and it is being inoculated with
The inoculation hole is sealed afterwards, the present invention is equipped with the edges inoculation port lid 1-6, inoculation port lid 1-6 on inoculation hole 1-3 and sets
It is useful for that the inside and outside buckle 1-7 that port lid is fixed on vessel will be planted, inside and outside buckle is divided into interior buckle it is preferably provided as 3 pairs
With outer buckle（See Fig. 6）, it is interior to be buckled in vessel inside sidewalls, it is outer to be buckled in vessel side-wall outer side.
Shading designed by the present invention is kept off the rain dust cover 2, is made preferably with black and white reflecting film material, white black outwardly
Inwardly.The upper end is equipped with elastic tying 2-1, and lower end is equipped on water outlet 2-2, elastic tying 2-1 and is equipped with tied rope 2-3；It ties up
The 2-3 that restricts is preferably stretch cord, ties up to nursery stock rhizome portion, elastic suitable for avoiding loose rainwater from flowing into, and tension is also avoided to be unfavorable for planting
Object is grown.Because cover body wraps vessel, in vessel chassis side, there are gaps（That is water outlet 2-2）Water supply and sewage is kept away
Exempt from root system and seedling medium to be in direct contact with nursery lot, indigenous Va Mycorrhiza Seedling travels in seedling medium.Shading is kept off the rain dust cover
Design avoid and infect non-purpose VA Mycorrhizal Fungi, while black and white reflective plastic film through media such as rainwater, wind and nursery lot soil
Illumination can be reflected into nursery stock leaf portion, increase photosynthesis, while reducing base is influenced to the direct projection of matrix due to illumination
Scattering and disappearing for moisture spends the high injury to VA Mycorrhizal Fungi and root system with matrix medium temperature in matter.
Water guider 3 designed by the present invention is shown in Fig. 5, and water guider 3 is by hollow bend pipe 3-1 and is secured to connection
Three foot guide support 3-2 are constituted, and the lower section of the three feet guide support 3-2 is water guide groove 3-3.In use, by bend pipe 3-1 into
Mouth of a river end connects water pipe for drip irrigation, that is, the trickle irrigation of osmosis type is used to water, slower using water-seepage type irrigation flow, to root system and mycelia
Development impact is smaller.It irrigates once, the long period without pouring, preferably maintains the moisture content of seedling medium, keeps nursery stock
The stability of root growth environment reduces because moisture changes the influence to VA Mycorrhizal Fungi and root system, three after being especially just inoculated with
A month.On the one hand the structure plays the role of giving Va Mycorrhiza Seedling watering, on the other hand playing the support shading dust cover that keeps off the rain prevents rain
Aqueous reflux enters the effect of container.
Using above-mentioned apparatus carry out mycorrhiza fungi seeding cultivating, cultural method include the formula of seedling medium, bush mycorrhiza agent preparation,
The cultivation of aseptic seedling, the inoculation of aseptic seedling, cultivation of Va Mycorrhiza Seedling etc..
Key step is as follows：
1）Cultivate aseptic seedling：Vegetable seeds for nursery drains away the water after fully being impregnated with tap water, removes seed inferior, uses
0.1% potassium permanganate impregnates 1 hour and carries out surface sterilization, and the appearance equipped with seedling medium is seeded in after aseptic water washing three times
It in device ontology, is cultivated under soil humidity 60-70%, sterile water pours；Seedling medium need to sterilize 1h in 121 DEG C, 0.1MPa.It should
Stage cultivates in greenhouse, greenhouse or laboratory.Certainly, long in aseptic seedling in greenhouse, greenhouse or laboratory when the anxiety of place
To certain altitude, the shading dust cover that keeps off the rain can also be put on and removed to outside scenery；
2）It is prepared by microbial inoculum：It is inoculum or using spore as the microbial inoculum of inoculum to prepare using mycelium；Microbial inoculum can be solid fungicide
Or liquid bacterial agent, wherein liquid bacterial agent are better.It is as follows as the bacterial preparation process of inoculum using mycelium：Mycelium is through liquid
Body expand it is numerous after, obtain bacterium ball after filtering, bacterium ball and nutrient solution by volume 1:1 is uniformly mixed, every 1000 milliliters of bacterium ball nutrient solution
Add 3-5g sodium alginates, stirs evenly；
3）Aseptic seedling is inoculated with：After aseptic seedling has lateral root development, inoculation port lid 1-6 is removed, at the inoculation hole 1-2 of vessel
Microbial inoculum is accessed after being punched using card punch, is fixed at inoculation hole by inside and outside buckle 1-7 by port lid 1-6 is inoculated with, i.e., to inoculation
Hole is sealed and is fixed on vessel, and then direction stromal surface pours into a mould nutrient solution from container；And then, in container
Sterilized seedling medium is added in ontology 1 again until filling vessel and slightly at steamed bun shape, then again in seedling medium
Upper storing water guider 3, it is desirable that the stem of bend pipe 3-1 and aseptic seedling reclines, then on vessel 1 cover shading keep off the rain it is dust-proof
Cover 2, the aerial part of aseptic seedling and the bend pipe 3-1 of water guider 3 keep off the rain dust cover 2 across elastic tying 2-1 positioned at shading
Outside, fasten tied rope 2-3 at elastic tying 2-1 with tied rope 2-3；See Fig. 1-3.Card punch of the present invention（See figure
7-8）, design as follows：Card punch is constituted by punching end 4-1 and handle end 4-2 two parts, and the punching end is flat sharp contract type
（Like axe）, a length of 5-6cm, width 1.5-1.8cm, handle end is cylinder, a length of 8-12cm, a diameter of 1.5-1.8cm；It adopts
Lateral root can be suitably cut off with sharp card punch or hurts cortical tissue, be conducive to mycelia and infected root system.The inoculation method is i.e. to seedling
Wood growth is unaffected, can also greatly improve mycorhiza infection rate；
5）The cultivation of Va Mycorrhiza Seedling：Aseptic seedling after inoculation removes greenhouse or greenhouse, is placed under natural environmental condition and cultivates, by leading
3 entrance of water installations injects sterile water；The sterile water is 15m underground water below from depth.In use, by bend pipe 3-1
Inlet end connects water pipe for drip irrigation, that is, the trickle irrigation of osmosis type is used to water.
The present invention is suitable for the symbiosis seedling culture of such as bolete, delicious lactarius, ferfas edible mycorrhizal fungi type, main
Sport technique segment be：（1）Symbiosis seeds appropriate are selected according to the characteristic of mycorhiza edible mushroom, such as suillus lutens and pine breast
The symbiosis seeds of mushroom are wet-land pine tree, masson pine etc.；The symbiosis host plant of ferfas is pecan tree, Chinese chestnut, pinus yunnanensis etc.；
（2）Cultivating aseptic seedling container uses this patent vessel, matrix that high-temperature sterilization, water source is used to come from 15m or less underground water, broadcast
Kind to reach inoculation require during in greenhouse or indoor culture；（3）It waits for that aseptic seedling reaches inoculation and requires, is filled out using card punch punching
Microbial inoculum is filled, addition nutrient solution induces microbial inoculum continued growth, increases touch opportunity and the time of root system and strain；（4）Use this hair
Bright culture apparatus, the cultural method after proper use of inoculation and operating procedure；（5）The cultivating aseptic seedling phase is indoors or in greenhouse
Cultivate, it is to be seeded after capping shading keep off the rain dust cover and water guider, under open-air condition full exposure cultivate.The present invention is more general
Cultivation container Va Mycorrhiza Seedling can substantially reduce the dip dyeing of the surging VA Mycorrhizal Fungi such as Lasiosphaera fenzlii, and miscellaneous bacteria infection rate obviously lowers, and purpose mycorhiza closes
It is greatly improved at rate, the Va Mycorrhiza Seedling that high quality is produced for scale, low cost provides technical guarantee.
Embodiment 2
The masson pine symbiosis seedling of orange newborn mushroom is inoculated with and cultivated using the device in embodiment 1, and method is as follows：
1, cultivating aseptic seedling
Masson pine（Pinus massonianaLamb.）Seed drains away the water after fully being impregnated with tap water, inferior kind of removal
Son impregnates 1 hour with 0.1% potassium permanganate and carries out surface sterilization, is sowed at equipped with seedling medium after aseptic water washing three times
It in vessel, is cultivated under soil humidity 60-70%, watering pours water is phreatic water；Seedling medium need to go out in 121 DEG C, 0.1MPa
Bacterium 1h.Cultivating aseptic seedling season is 5-6 months.It is cultivated in greenhouse or greenhouse.Seedling medium formula is vermiculite：Sandy loam=1:1
（Volume ratio）, natural pH value.
2, prepared by microbial inoculum, and method is as follows
1）Strain tissue detaches：The orange newborn mushroom of acquisition in Lishui City Jinyun county Flora of Dayang Mountain forest zone(Lactarius akahatsu) son
Entity takes back laboratory and carries out tissue separation, takes cap and stem cohesive position mung bean size tissue, places special solid culture
The purifying culture of picking mycelia is spare after cultivating a week on base.The special solid culture medium prescription is：Potato 200g is boiled
Juice, glucose 20g, brown sugar 5g, 1.5 g of potassium dihydrogen phosphate, 1 g of magnesium sulfate, 1 g of ammonium chloride, peptone 1g, 1% lemon are filtered to take in boiling
Lemon acid iron 0.5ml, 0.1% zinc sulfate 1ml, vitamin B1 10mg, agar 20g add water to be settled to 1000ml；
2）Strain expands numerous：Cultured strain is taken, 1cm is cut with scalpel25 pieces of strain block, which is put into liquid shaking bottle, to be cultivated, liquid
Body culture medium prescription：Potato 200g, which boils, filters to take juice, glucose 20g, brown sugar 5g, 1.5 g of potassium dihydrogen phosphate, magnesium sulfate 1
G, 1 g of ammonium chloride, peptone 1g, 1% ironic citrate 0.5ml, 0.1% zinc sulfate 1ml, vitamin B1 10mg add water to be settled to
1000ml；
Using 500ml shaking flasks, liquid amount 300ml, it is spare to cultivate 25d for 25 DEG C, rotating speed 140r/min of cultivation temperature；
3）It is prepared by liquid bacterial agent：Through liquid expand it is numerous after the filtered bacterium ball of mycelium and nutrient solution（It is formulated the liquid with step 2
Culture medium）By volume 1:1, every 1000 milliliters plus 3-5g of sodium alginate crushing, which is mixed thoroughly, is made mycelium liquid microbial inoculum.
3, it is inoculated with：It is inoculated in month autumn and winter 10-11, inoculation method is：After masson pine aseptic seedling has lateral root development, remove
It is inoculated with port lid 1-6,5-10cm punchings are entered using card punch downward oblique cutting at inoculation hole at the inoculation hole 1-2 of vessel,
The orange newborn mushroom liquid bacterial agent 5ml-10ml of access per hole；It will be inoculated with port lid 1-6 by inside and outside buckle 1-7 and be fixed on envelope at inoculation hole
Mouthful, seedling medium is uniformly poured in portion from container spreads nutrient solution about 300-500ml.
4, seedling medium is added：That sterilized nursery base is added above the seedling medium for spreading overnutrition liquid again is upright pouring
To vessel is filled, keep stromal surface slightly at steamed bun shape.
5, water guider and shading are installed keep off the rain dust cover：Water guider is put on the seedling medium slightly at steamed bun shape,
It is required that the stem of bend pipe and aseptic seedling reclines, shading is then covered on vessel and is kept off the rain dust cover, the rhizome of aseptic seedling and
The bend pipe of water guider keeps off the rain the outside of dust cover across elastic tying and positioned at shading, is at elastic tying 2-1 with tied rope
Upper tied rope.
, mycorrhiza fungi seeding cultivating：By install water guider and shading keep off the rain dust cover device Va Mycorrhiza Seedling be placed on it is outdoor
Under the conditions of cultivate；2 week did not watered after inoculation, and backsight water content of substrate situation pours moisture, and water content of substrate is kept to exist
It is cultivated under the conditions of 60-70%；It pours water source and comes from Deep Underground water.It is the undergrounds below 15m that phreatic water, which is preferably derived from depth,
Water.It cultivates 6-12 months, the masson pine seedling radicula surface mycelia of inoculation covers to be formed, and mycorrhizal morphology is two forked, rodlike, corals
Shape, rufous, are shown in Fig. 9.
Through investigation, under open-air condition garden nursery mycorhiza infection conditions be shown in Table 1.The results show that using common vessel nursery,
Miscellaneous bacteria infection rate is up to 41.1%, wherein Lasiosphaera fenzlii class infection rate is up to 30.6%.And the device miscellaneous bacteria infection rate used in this patent is
0.96%, Lasiosphaera fenzlii class infection rate is 0.Miscellaneous bacteria infection rate significantly lowers, and the masson pine of high quality is produced for scale, low cost
Va Mycorrhiza Seedling provides technical guarantee.
Ground nursery mycorhiza infection conditions table in garden under 1 open-air condition of table
Claims (9)
1. a kind of method for cultivating orange newborn mushroom offspring, it is characterised in that：Include the following steps：
1）Prepare device for raising seedlings：The device includes vessel（1）, shading keeps off the rain dust cover（2）And water guider（3）；
The vessel（1）Side wall is equipped with air hole（1-1）And inoculation hole（1-2）, vessel（1）Side wall and bottom surface
Junction is provided inwardly with step（1-3）, orifice plate is equipped on step（1-5）, the container sheet between bottom surface and orifice plate
Body sidewall is equipped with osculum（1-4）；
The shading is kept off the rain dust cover（2）Upper end is equipped with elastic tying（2-1）, lower end is equipped with water outlet（2-2）, elastic tying
（2-1）On be equipped with tied rope（2-3）；The shading is kept off the rain dust cover（2）It is made using black and white reflecting film material, white
Black is inwardly outwardly；
The water guider（3）By hollow bend pipe（3-1）With the three foot guide supports for being secured to connection（3-2）It constitutes, institute
State three foot guide supports（3-2）Lower section be water guide groove（3-3）；The inoculation hole（1-3）On be equipped with inoculation port lid（1-
6）, it is inoculated with port lid（1-6）Edge is equipped with is fixed on vessel for that will plant port lid（1）On inside and outside buckle（1-7）；
2）Cultivating aseptic seedling：In vessel（1）Interior cultivation masson pine aseptic seedling；
3）It is prepared by microbial inoculum：Prepare orange newborn mushroom liquid bacterial agent；
4）Inoculation：It is inoculated in month autumn and winter 10-11, inoculation method is：After masson pine aseptic seedling has lateral root development, inoculation is removed
Port lid（1-6）, in the inoculation hole of vessel（1-2）Place enters 5-10cm punchings using card punch downward oblique cutting at inoculation hole,
The orange newborn mushroom liquid bacterial agent 5-10ml of access per hole；Pass through inside and outside buckle（1-7）Port lid will be inoculated with（1-6）It is fixed at inoculation hole
Sealing, seedling medium is uniformly poured in portion from container spreads nutrient solution about 300-500ml；
5）Seedling medium is added：Sterilized seedling medium is added above the seedling medium for spreading overnutrition liquid again until dress pouring
Full vessel（1）, keep stromal surface slightly at steamed bun shape；
6）Installation water guider and shading are kept off the rain dust cover：Water guider is put on the seedling medium slightly at steamed bun shape（3）,
Seek bend pipe（3-1）It reclines with the stem of aseptic seedling, then in vessel（1）Upper covering shading is kept off the rain dust cover（2）, aseptic seedling
Rhizome and water guider（3）Bend pipe（3-1）Across elastic tying（2-1）And it keeps off the rain dust cover positioned at shading（2）Outside,
Use tied rope（2-3）In elastic tying（2-1）Fasten tied rope in place（4）；
7）Mycorrhiza fungi seeding cultivating：Water guider will be installed and the keep off the rain Va Mycorrhiza Seedling of device of dust cover of shading is placed on open-air condition
Lower culture；2 week did not watered after inoculation, and backsight water content of substrate situation pours moisture, kept water content of substrate in 60-
It is cultivated under the conditions of 70%；It pours water source and comes from Deep Underground water.
2. a kind of method for cultivating orange newborn mushroom offspring according to claim 1, it is characterised in that step 2）Middle horse hair
The breeding method of loose aseptic seedling is：Masson pine seed is taken, is drained away the water after fully being impregnated with tap water, seed inferior is removed, uses
0.1% potassium permanganate impregnates 1 hour and carries out surface sterilization, and the container equipped with seedling medium is seeded in after aseptic water washing 3 times
Ontology（1）It is interior, water content of substrate 60-70% is kept, is cultivated in greenhouse or greenhouse；Water source is phreatic water；Seedling medium need to be
121 DEG C, sterilize in 0.1MPa 1h.
3. a kind of method for cultivating orange newborn mushroom offspring according to claim 2, it is characterised in that：The seedling medium
Formula is vermiculite：Sandy loam=1:1（Volume ratio）, natural pH value.
4. a kind of method for cultivating orange newborn mushroom offspring according to claim 1, it is characterised in that step 3）In it is orange
Newborn mushroom liquid bacterial agent preparation method is as follows：
1）Strain tissue detaches：Orange newborn massee fruiting bodies are acquired, laboratory is taken back and carries out tissue separation, cap is taken to be connected with stem
Position mung bean size tissue, the purifying culture of picking mycelia is spare after a week is cultivated on solid medium；
2）Strain expands numerous：Cultured strain is taken, strain block is taken to be put into shaking flask culture in fluid nutrient medium, 25 DEG C of cultivation temperature,
Rotating speed 140r/min cultivates 25d, spare；
3）It is prepared by liquid bacterial agent：Take step 2）Bacterium ball is obtained by filtration in middle bacterium solution, and fluid nutrient medium, bacterium ball and fluid nutrient medium is added
By volume 1:1 mixing；Then sodium alginate is added in mixed liquor, crushing, which is mixed thoroughly, is made liquid bacterial agent；The sodium alginate
The amount of 3-5g is added to be added by every 1L mixed liquors.
5. a kind of method for cultivating orange newborn mushroom offspring according to claim 4, it is characterised in that：The Liquid Culture
Based formulas：Potato 200g, which boils, filters to take juice, glucose 20g, brown sugar 5g, 1.5 g of potassium dihydrogen phosphate, 1 g of magnesium sulfate, chlorination
1 g of ammonium, peptone 1g, 1% ironic citrate 0.5ml, 0.1% zinc sulfate 1ml, vitamin B1 10mg add water to be settled to 1000ml；
The solid culture based formulas is added agar 20g by every 1L by aforesaid liquid culture medium and is made.
6. a kind of method for cultivating orange newborn mushroom offspring according to claim 1, it is characterised in that：Described in step 4)
Card punch by punch end（4-1）And handle end（4-2）Two parts are constituted, and the punching end is flat sharp contract type, a length of 5-
6cm, width 1.5-1.8cm, handle end are cylinder, a length of 8-12cm, a diameter of 1.5-1.8cm.
7. a kind of method of the orange newborn mushroom offspring of cultivation according to claim 1, it is characterised in that：The appearance
Device ontology bottom surface and orifice plate（1-5）The distance between be 1-2cm, i.e. step（1-3）Height be 1-2cm.
8. a kind of method of the orange newborn mushroom offspring of cultivation according to claim 1 or 2, it is characterised in that：Institute
It is 15m underground water below that phreatic water, which is stated, from depth.
9. a kind of method of the orange newborn mushroom offspring of cultivation according to claim 1, it is characterised in that：The appearance
Inoculation hole on this body sidewall of device is round, and bore dia 1.5-2cm, inoculation hole is arranged 5, is uniformly distributed in a ring, and setting exists
The middle and upper part of vessel.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN117678511A (en) * | 2024-02-02 | 2024-03-12 | 中国科学院昆明植物研究所 | Method for preparing mycorrhizal seedlings by inoculating fluid strain of Yunnan manyleaf puffball into hickory nuts in America |
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