CN107047061A - The high efficiency method and its culture medium of cultivation of glossy ganoderma - Google Patents
The high efficiency method and its culture medium of cultivation of glossy ganoderma Download PDFInfo
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- 241000222336 Ganoderma Species 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 23
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- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 73
- 239000007788 liquid Substances 0.000 claims abstract description 64
- 238000000855 fermentation Methods 0.000 claims abstract description 49
- 230000004151 fermentation Effects 0.000 claims abstract description 49
- 240000008397 Ganoderma lucidum Species 0.000 claims abstract description 42
- 235000001637 Ganoderma lucidum Nutrition 0.000 claims abstract description 42
- 238000011081 inoculation Methods 0.000 claims abstract description 40
- 244000061456 Solanum tuberosum Species 0.000 claims abstract description 17
- 235000002595 Solanum tuberosum Nutrition 0.000 claims abstract description 17
- 235000010643 Leucaena leucocephala Nutrition 0.000 claims abstract description 16
- 240000007472 Leucaena leucocephala Species 0.000 claims abstract description 14
- 238000011534 incubation Methods 0.000 claims abstract description 14
- 244000068988 Glycine max Species 0.000 claims abstract description 10
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 10
- 239000002075 main ingredient Substances 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 43
- 239000000243 solution Substances 0.000 claims description 38
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- 235000015097 nutrients Nutrition 0.000 claims description 27
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- 238000003306 harvesting Methods 0.000 claims description 22
- 239000007787 solid Substances 0.000 claims description 22
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- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 16
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- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims description 8
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 8
- PASHVRUKOFIRIK-UHFFFAOYSA-L calcium sulfate dihydrate Chemical compound O.O.[Ca+2].[O-]S([O-])(=O)=O PASHVRUKOFIRIK-UHFFFAOYSA-L 0.000 claims description 8
- 239000002609 medium Substances 0.000 claims description 8
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- VQKFNUFAXTZWDK-UHFFFAOYSA-N alpha-methylfuran Natural products CC1=CC=CO1 VQKFNUFAXTZWDK-UHFFFAOYSA-N 0.000 claims description 2
- 235000009508 confectionery Nutrition 0.000 claims description 2
- 235000013305 food Nutrition 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 239000011591 potassium Substances 0.000 claims description 2
- 229910052700 potassium Inorganic materials 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 4
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- 239000002361 compost Substances 0.000 description 4
- 239000001888 Peptone Substances 0.000 description 3
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- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 2
- ABUGVBRDFWGJRD-CHOYNLESSA-N [9-[(2r,3r,4s,5r)-3,4-dihydroxy-5-methyloxolan-2-yl]-2-(2,4-dinitrophenyl)sulfanylpurin-6-yl] [hydroxy(phosphonooxy)phosphoryl] hydrogen phosphate Chemical compound O[C@@H]1[C@H](O)[C@@H](C)O[C@H]1N1C2=NC(SC=3C(=CC(=CC=3)[N+]([O-])=O)[N+]([O-])=O)=NC(OP(O)(=O)OP(O)(=O)OP(O)(O)=O)=C2N=C1 ABUGVBRDFWGJRD-CHOYNLESSA-N 0.000 description 2
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- 239000012138 yeast extract Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
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- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
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- 229910052788 barium Inorganic materials 0.000 description 1
- DSAJWYNOEDNPEQ-UHFFFAOYSA-N barium atom Chemical compound [Ba] DSAJWYNOEDNPEQ-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 210000000748 cardiovascular system Anatomy 0.000 description 1
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- 239000002054 inoculum Substances 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 229910052750 molybdenum Inorganic materials 0.000 description 1
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- 239000011669 selenium Substances 0.000 description 1
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- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 150000003648 triterpenes Chemical class 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Mycology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Pest Control & Pesticides (AREA)
- Environmental Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses the efficient culture medium of cultivation of glossy ganoderma, including the culture medium A for liquid Mother culture and the culture medium B for fermentor liquid Spawn incubation；Culture medium A is using potato, mushroom carpophore as Main Ingredients and Appearance, and culture medium B gives up bacteria residue as Main Ingredients and Appearance using soya bean, mushroom carpophore, ganoderma lucidum fruiting bag.Accordingly, inventor has also set up corresponding cultural method, in the method：Fruiting bag culture medium adds surface suction material resources with the larger organic material of adsorption area to increase the adhesive force and restoration ecosystem power of liquid spawn based on acacia rachii branch by acacia rachii branch media surface；Meanwhile, inventor is improved fermentation tank and classification inoculation apparatus.Original liquid spawn formula can be substituted using the technical system of the present invention, and makes ferment tank success rate up to 100%, bacterium bag inoculation survival rate 100% shortens the mycelia production cycle, improves yield, reduces cost.
Description
Technical field
The invention belongs to the high efficiency method and its culture medium in cultivation of glossy ganoderma field, more particularly to a kind of cultivation of glossy ganoderma.
Background technology
Ganoderma lucidum (Ganoderma Lucidum Karst) is a kind of medical value and nutritive value higher medical, edible
Bacterium, is always treated as the Chinese medicine of preciousness since ancient times.Modern pharmacological research shows that ganoderma lucidum has enhancing immunity of organisms, resists
Tumour, protecting liver and detoxication improves cardiovascular system, the effects such as antiviral.The efficacy component of ganoderma lucidum mainly has GL-B class, triterpene
Class, ucleosides, amino acid etc., separately also containing various trace elements such as molybdenum, zinc, manganese, iron, selenium, barium.The spirit of large-scale pseudo-wild cultivating
Not only yield and medical value are higher than general small ganoderma lucidum to sesame, and with viewing and admiring reserve value.It is main present in ganoderma lucidum production
Problem is that the need for strain quality can not meet ganoderma lucidum production development with quantity, current southern area ganoderma lucidum bacterium bag production generally makes
With solid spawn, it is using the shortcoming of solid spawn：Material feeding is slow after strain itself growth period length (30 days), not shelf-stable, inoculation.
And liquid spawn has growth cycle short (300L strain cultivations only need 6 days standards that can reach for production), cell age
Unanimously, the features such as developing homogeneous and neat healthy and strong, overcomes the deficiency of solid spawn.
Nevertheless, still there are various problems in application process in Liquid Strain of Ganoderma Lucidum：
(1) need to add the compositions such as yeast extract, peptone in fluid nutrient medium, therefore culture medium cost is high；
(2) many Edible Fungi factories are all in suburb and remote countryside, and supply of electric power is unstable, met during liquid fermentation
To when having a power failure, whole fermentation tank bacterium solution is scrapped with regard to whole pollution microbes；
(3) fermentation tank belongs to high-pressure disinfection equipment, generally with transfer room, culturing room not in a region.Fermentation tank is produced
The substantial amounts of liquid spawn movement gone out is inconvenient, common at present using the method inoculation for lengthening drain pipe, or when bacterium bag is inoculated with
Move near fermentation tank, connect and moved cultivation region to, both of these approaches both increases the pollution rate of bacterium bag；
(4) when high-quality ganoderma lucidum pseudo-wild cultivating uses acacia rachii branch for material, liquid spawn is directly inoculated in yearning between lovers
It is close by wood structure when on branch bar, it is unfavorable for liquid spawn attachment, acacia rachii shoot vegetative composition is generally macromolecular in addition
Lignin, cellulose, are also unfavorable for the short-term quick restoration ecosystem of liquid spawn, thus bacterium bag survival rate is relatively low.
The content of the invention
The technical problem to be solved in the invention is to provide that a kind of production cost is low, probability is small for pollution, nutrition is good and mycelia
The high efficiency method and its culture medium for the cultivation of glossy ganoderma that growth period is short, yield is high.
In order to solve the above technical problems, the present invention uses following technical scheme：
The efficient culture medium of cultivation of glossy ganoderma, including culture medium A for liquid Mother culture and for fermentor liquid bacterium
Plant the culture medium B of culture；Culture medium A using potato, mushroom carpophore as Main Ingredients and Appearance, culture medium B with soya bean, mushroom carpophore,
Ganoderma lucidum fruiting bag gives up bacteria residue for Main Ingredients and Appearance.
The efficient culture medium of cultivation of glossy ganoderma, including culture medium A, B, C, D, wherein：
The formula of culture medium A is per useless containing potato 200.0g, rice bran 50.0g, ganoderma lucidum fruiting bag in 1000ml nutrient solutions
Bacteria residue 50.0g, mushroom carpophore 10.0g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 1.5g, sucrose 25.0g, water 700-900ml are fixed
Hold to 1000ml；
Culture medium B formula is per useless containing potato 100.0g, rice bran 50.0g, ganoderma lucidum fruiting bag in 1000ml nutrient solutions
Bacteria residue 50.0g, mushroom carpophore 40.0g, analysis for soybean powder 5.0g, potassium dihydrogen phosphate 6.0g, magnesium sulfate 2.5g, glucose 10.0g, hair
Ferment food defoamer 0.1ml, water 700-900ml, are settled to 1000ml；
The formula of culture medium C be per 1000g culture mediums in given up bacteria residue containing cotton seed hull 400.0g, ganoderma lucidum fruiting bag
370.0g, bagasse 100.0g, corncob 100.0g, land plaster 10.0g, calcium superphosphate 10.0g, potassium dihydrogen phosphate 10.0g, training
Foster base adds water 600-700ml；
Culture medium D formula be per 1000g culture mediums in contain acacia rachii branch 1000g, culture medium adds water 600-
700ml。
Culture medium A is prepared by following operation：By potato, rice bran, ganoderma lucidum fruiting bag give up bacteria residue, this 4 kinds of mushroom carpophore into
Divide and mix, blend, add water 700ml, boil to boiling, maintain boiling 10 minutes, filtering is removed slag, stays filtered juice；By biphosphate
Potassium, magnesium sulfate, sucrose add filtrate, mix dissolving；Adding water makes final culture volume reach 1000ml；
Culture medium B is prepared by following operation：By potato, rice bran, ganoderma lucidum fruiting bag useless bacteria residue, mushroom carpophore, analysis for soybean powder
This 5 kinds of compositions, which mix, to be mixed, is boiled to boiling, filtering, remove slag reserved filtrate；By defoamer, potassium dihydrogen phosphate, magnesium sulfate, Portugal
Grape sugar, which is added in appropriate water, to be dissolved；Filtrate and the aqueous solution are mixed, and adding water makes final nutrient solution volume reach 1000ml；
Culture medium C is prepared by following operation：By cotton seed hull, ganoderma lucidum fruiting bag useless bacteria residue, bagasse, corncob, land plaster
This 5 kinds of compositions, which mix, to be mixed；Calcium superphosphate, potassium dihydrogen phosphate are dissolved in appropriate water；By mixture and the aqueous solution
Mixing, and adding water makes final moisture content in medium reach 65%；
Culture medium D is prepared by following operation：By acacia rachii branch wood parison water 12-15 hours to water-holding capacity up to 65%.
The high efficiency method of cultivation of glossy ganoderma is carried out using above-mentioned culture medium, culture medium A is used for liquid Mother culture, will be cultivated
Base B is used for fermentor liquid Spawn incubation, and culture medium C is used for into the activated growth that rigid connection enters the liquid spawn of fruiting bag, will be trained
Supporting base D is used for fruiting bag Mycelium culture.
The high efficiency method of above-mentioned cultivation of glossy ganoderma, comprises the following steps：
(1) culture medium A is sub-packed in 1000ml vial, every bottle of nutrient solution 500ml, 120 DEG C sterilize 60 minutes, sterilizing
Wild Oryza species are cooled to room temperature, access the solid parent species of 1.0cm × 1.0cm × 1.0cm sizes, 25-28 DEG C of shaking table training at room temperature
Support, shaking table shakes 150 revs/min of speed, and incubation time 5 days obtains the liquid parent species that mycelial density accounts for nutrient solution 80%；
(2) culture medium B is sub-packed in 450L fermentation tank, per canned nutrient solution 300L, 60 minutes at 120 DEG C, after sterilizing
Nutrient solution is cooled to room temperature, accesses 1000ml liquid parent species, 28 DEG C are cultivated at room temperature, maintains tank pressure 0.03-0.05mPa, and gas is built
Pressure gauge 0.15mPa, incubation time 5-6 days obtains the liquid spawn that mycelium pellet density accounts for 80%；
(3) after fermentor liquid culture medium inoculated culture 24 hours, be measured by sampling every 12 hours from sample tap whether there is it is miscellaneous
Bacterium infects；
(4) the culture medium D prepared is sub-packed in 40cm × 60cm polypropylene plastics pockets, per packed siccative 4000g, in training
The culture medium C that base D surfaces add 80g is supported, surface of tiling ties sack, sterilization 240 minutes, then take out at 120 DEG C,
It is cooled to room temperature stand-by；Transfer room inoculation is moved into, every bag is accessed 80ml liquid spawns, and 28 DEG C are cultivated at room temperature, incubation time 60-
65 days, obtain covering with the fruiting bag of mycelia；
(5) bacterium bag for covering with mycelia is peelled off into polybag, the good greenhouse of ground sterilization or sylvan life is put in, in bacterium bag surface cover
One layer of plastic film, room temperature is controlled at 25-28 DEG C, is maintained 5-7 days, and it is secondary with ventilation during which to raise film daily, every time
Raise the time 10 minutes；
(6) the bacterium bag surface earthing of ripe effect in the completed, soil layer 2-3CM is thick, pours once permeable after earthing, later every
Drench a water within 3-4 days；
(7) 28-35 DEG C of mushroom house temperature, air humidity 80-90%, before the bacterium bag mushroom flower bud of covering Nutrition Soil is not unearthed, often
A water was drenched every 3-4 days；After more than 50% bacterium bag has mushroom flower bud to be formed, drench within 3 days once permeable, until harvesting；After every batch is adopted
Stop water spray 5-7 days.
It is provided with the blast pipe of fermentation tank at two non-return valves, the air pump of fermentation tank and a Pyatyi filter is installed
Gas tank.
Inoculation is using portable inoculation kettle in step (4), and movable type inoculation kettle is main by being connected successively by anti-high pressure rubber pipe
The gas connect is built, filter, stainless steel storage bacterium solution container, inoculating gun composition, and storage bacterium solution container top sets dress liquid mouth, sidepiece and sets the back of the body
Band.
The problem of existing for existing cultivation of glossy ganoderma, inventor gives up bacteria residue as object using mushroom carpophore, ganoderma lucidum fruiting bag,
Studied by different materials composite test, have developed the efficient culture medium of the cultivation of glossy ganoderma of inexpensive high yield, including for liquid
The culture medium A of Mother culture and the culture medium B for fermentor liquid Spawn incubation；Culture medium A using potato, mushroom carpophore as
Main Ingredients and Appearance, culture medium B gives up bacteria residue as Main Ingredients and Appearance using soya bean, mushroom carpophore, ganoderma lucidum fruiting bag.Wherein, flat mushroom is a kind of
Relatively common cultivation scope is wide, the edible fungus variety that yield is high, price is low, and its new fresh sporophore protein content reaches 5-
6%, polyoses content reaches 2-3% in dry product, and mushroom carpophore is added in liquid bacteria in culture medium as proteinaceous nutrient, had
Beneficial to reduction culture medium cost, simultaneously as flat mushroom belongs to fungi together with ganoderma lucidum, nutrient utilization has additive effect.Accordingly, send out
A person of good sense has also set up corresponding cultural method, in the method：Fruiting bag culture medium passes through acacia rachii based on acacia rachii branch branch
Inhale material resources and the larger organic material of adsorption area (liquid spawn activation auxiliary compost cotton in branch media surface addition surface
Sub- shell, corn flour, ganoderma lucidum fruiting bag give up bacteria residue etc.) increase the adhesive force and restoration ecosystem power of liquid spawn；Meanwhile, inventor
Fermentation tank and classification inoculation apparatus are improved.Original liquid spawn formula can be substituted using the technical system of the present invention,
And making ferment tank success rate up to 100%, bacterium bag inoculation survival rate 100% shortens the mycelia production cycle, improves yield, drop
Low cost.
Relative to prior art, outstanding advantage of the invention is characterized in particular in：
(1) liquid spawn factory formula cost declines.Every 100 liters of nutrient solutions need to add yeast extract 250g when original formulation is produced,
Cost is 17.0 yuan (34 yuan/500g), after fluid present invention strain factory formula, and ferment is substituted with mushroom carpophore extract solution
Female cream, being now formulated every 100 liters of nutrient solutions need to add mushroom carpophore 4.0Kg, 5.0 yuan/Kg of flat mushroom market price to calculate, mushroom carpophore
20.0 yuan of 4.0Kg costs, than control reduction by 41.2%.
(2) when red ganoderma uses solid spawn, parent species growth, which is needed 10 days, original seed grows needs 30 days (bacterium bottle specifications：25×
20CM), cultigen growth needs 25 days (bacterium bottle specifications：25 × 20cm), fruiting bag growth needs 90 days (bacterium bag specifications：40×
60cm), totally 155 days, First Year biological transformation ratio 25.5%；After liquid spawn using the present invention, liquid parent species need 7 days, hair
The growth of fermentation tank liquid spawn is needed 5-7 days, and fruiting bag growth is needed 60-65 days, and common 72-79 days, only fruiting bag growth phase was just compared
According to shortening 30-25 days, First Year biological transformation ratio 32.2% improves 25.8% than control.
(3) when using the progress liquid spawn production of shape fermentation tank is not changed, strain cultivation success rate 20%.Using repacking
When shape fermentation tank carries out liquid spawn production afterwards, caused ambient atmos returning to fermentation tank when can prevent to have a power failure suddenly
Stream；Strain cultivation success rate 100%, pollution rate is 0.It is inoculated with, is exempted from using the mobile liquid inoculation kettle invented
Go bacterium bag to move to fermentation tank the labor cost moved back to after inoculation and bacterium bag inoculation used in culture room, solve liquid bacteria
Transportation problem during inoculation is planted, inoculation efficiency is 1270 bags/6 people/hour, bacterium bag pollution rate 1.5%.
When red ganoderma uses solid spawn, inoculation efficiency is 490 bags/6 people/hour, bacterium bag pollution rate 4.2%.Using this
After the liquid spawn of invention, inoculation efficiency is 1270 bags/6 people/hour, and bacterium bag pollution rate 1.5%, inoculation efficiency is improved than control
159.2%；Bacterium bag pollution rate declines 64.2%.
The ganoderma lucidum bacterium bag production time shortens, and biological transformation ratio is improved.Connect using improved fermentation tank of the invention, movement
Plant kettle, fermentation liquor formulation and carry out liquid spawn production and application, the ganoderma lucidum fruiting bacterium bag production time, by original 90-100 days, contracts
It is short to 60-65 days；Red ganoderma First Year biological transformation ratio has brought up to 32.2% by 25.6%, and increase rate is 25.8%；It is black
Ganoderma lucidum First Year biological transformation ratio has brought up to 25.1% by 22.6%, and increase rate is 11.1%.
Brief description of the drawings
Fig. 1 is portable is inoculated with kettle, figure：1 gas is built, 2 filters, 3 dress bacterium solution containers, 4 inoculating guns, 5 braces, 6 dress liquid
Mouthful.
During Fig. 2 is fermentation tank, figure：1 gas is built, 2 gas tanks, 3 Pyatyi filters, 4 fermentation tanks, 5 non-return valves, 6 blast pipes, 7
Steam vent.
Embodiment
The present invention of embodiment 1 is used for the cultivation of red ganoderma
(1) culture medium is prepared
Culture medium A is formulated：Contain potato 200.0g, rice bran 50.0g, the useless bacteria residue of ganoderma lucidum fruiting bag in per 1000ml nutrient solutions
50.0g, mushroom carpophore 10.0g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 1.5g, sucrose 25.0g, water 700-900ml are settled to
1000ml；
Culture medium A preparation method：Give up bacteria residue, mushroom carpophore this 4 kinds of compositions of potato, rice bran, ganoderma lucidum fruiting bag are mixed,
Blend, add water 700ml, boil to boiling, maintain boiling 10 minutes, filtering is removed slag, stays filtered juice；By potassium dihydrogen phosphate, magnesium sulfate,
Sucrose adds filtrate, mixes dissolving；Adding water makes final culture volume reach 1000ml；
Culture medium B is formulated：Contain potato 100.0g, rice bran 50.0g, the useless bacteria residue of ganoderma lucidum fruiting bag in per 1000ml nutrient solutions
50.0g, mushroom carpophore 40.0g, analysis for soybean powder 5.0g, potassium dihydrogen phosphate 6.0g, magnesium sulfate 2.5g, glucose 10.0g, fermentation food
Product defoamer (model DF-1209, the production of Guangdong De Feng defoamers Co., Ltd) 0.1ml, water 700-900ml, is settled to
1000ml；
Culture medium B preparation methods：Give up bacteria residue, mushroom carpophore, analysis for soybean powder this 5 kinds of compositions of potato, rice bran, ganoderma lucidum fruiting bag are mixed
Mix, boiled to boiling together, filtering, the reserved filtrate that removes slag (wherein potato is cut to thin slice before boiling, ganoderma lucidum fruiting bag give up bacteria residue, put down
Massee fruiting bodies are first crushed before boiling) defoamer, potassium dihydrogen phosphate, magnesium sulfate, glucose are added in appropriate water and dissolved；By filtrate
Mixed with the aqueous solution, and adding water makes final nutrient solution volume reach 1000ml；
Culture medium C is formulated：Given up bacteria residue 370.0g, sweet containing cotton seed hull 400.0g, ganoderma lucidum fruiting bag in per 1000g culture mediums
Bagasse 100.0g, corncob 100.0g, land plaster 10.0g, calcium superphosphate 10.0g, potassium dihydrogen phosphate 10.0g, culture medium add water
600-700ml；
Culture medium C preparation method：Give up bacteria residue, bagasse, corncob, land plaster this 5 kinds of compositions of cotton seed hull, ganoderma lucidum fruiting bag are mixed
Mix together；Calcium superphosphate, potassium dihydrogen phosphate are dissolved in appropriate water；Mixture and the aqueous solution are mixed, and added water
Final moisture content in medium is set to reach 65% or so；
Culture medium D is formulated：Contain acacia rachii branch 1000g in per 1000g culture mediums, culture medium adds water 600-700ml.
Culture medium D preparation methods：Soaked 12-15 hours of acacia rachii branch (diameter 8-10cm) material is left to water-holding capacity up to 65%
It is right.
(2) packing of culture medium, sterilization, inoculation
Culture medium A is sub-packed in 1000ml vial, every bottle of nutrient solution 500ml, 120 DEG C sterilize 60 minutes, after sterilizing
Culture medium is cooled to room temperature, accesses the solid parent species of about 1.0cm × 1.0cm × 1.0cm sizes, 25-28 DEG C of shaking table at room temperature
Culture, shaking table shakes 150 revs/min of speed, and incubation time 5 days obtains the liquid parent species that mycelial density accounts for nutrient solution 80% or so；
Culture medium B is sub-packed in 450L fermentation tank, per canned nutrient solution 300L, 60 minutes at 120 DEG C, cultivated after sterilizing
Liquid is cooled to room temperature, accesses about 1000ml liquid parent species, 28 DEG C are cultivated at room temperature, maintains tank pressure 0.03-0.05mPa, and gas is built
Pressure gauge 0.15mPa or so, incubation time 5-6 days obtains the liquid spawn that mycelium pellet density accounts for 80% or so；
The culture medium D prepared is sub-packed in 40cm × 60cm polypropylene plastics pockets, per packed siccative about 4000g, in training
The culture medium C that base D surfaces add about 80g is supported, surface of tiling ties sack, sterilization 240 minutes, Ran Houqu at 120 DEG C
Go out, be cooled to room temperature stand-by；Transfer room inoculation is moved into, every bag is accessed 80ml liquid spawns, and 28 DEG C are cultivated at room temperature, incubation time
60-65 days, obtain covering with the fruiting bag of mycelia；
(3) mycelia afterripening：The bacterium bag for covering with mycelia is peelled off into polybag, the good greenhouse of ground sterilization or woods is put in
Under, in one layer of plastic film of bacterium bag surface cover, room temperature is controlled at 25-28 DEG C, is maintained 5-7 days, film is during which raised daily secondary
With ventilation, the time is raised every time 10 minutes.
(4) earthing：The bacterium bag surface earthing of ripe effect in the completed, soil layer 2-3CM is thick, pours once permeable after earthing, with
A water was drenched every 3-4 days afterwards, makes soil layer surface wettability；
(5) ganoderma lucidum fruiting period management
28-35 DEG C of mushroom house temperature, air humidity 80-90%, before the bacterium bag mushroom flower bud of covering Nutrition Soil is not unearthed, every 3-
Drench a water within 4 days, make soil layer surface wettability；After more than 50% bacterium bag has mushroom flower bud to be formed, drench within 3 days once permeable, directly
To harvesting；Every batch adopt after stop water spray 5-7 days, to promote mycelia restoration ecosystem.
As a result
(1) this time experiment in cultivation production tank of fermentation tank 8, pollution rate is 0；
(2) ganoderma lucidum bacterium bag covering Nutrition Soil 15 days or so, 50% bacterium bag mushroom flower bud, existing 20 days or so of mushroom flower bud life, fructification into
It is ripe, harvesting；About 60 days or so after adopting for the first time, second batch mushroom flower bud is formed, and the life of mushroom flower bud is existing 20 days or so, and fructification is ripe, harvesting；
About 60 days or so after adopting for the second time, the 3rd batch of mushroom flower bud is formed, and the life of mushroom flower bud is existing 20 days or so, and fructification is ripe, harvesting.Adopted per batch
After receipts, fungus block surface is cleared up, earthing is mended again, is placed 7 days, after after bacterium life restoration ecosystem, management is equally repeated with early stage.First
Mushroom biological transformation ratio 12.8% is criticized, the 3rd batch of mushroom biological transformation ratio 9.9%, the 3rd batch of mushroom biological transformation ratio 9.5% harvests three altogether
Batch, the total biological transformation ratio 32.2% of First Year.
After (3) the 3rd batches are adopted, bacterium bag earthing is survived the winter, and is started to Second Year late March, recovers moisture content and air humidity pipe
Reason.Water management about 20 days or so, mushroom flower bud occurs, and after 20 days, fructification was ripe, harvesting；About 60 days or so after adopting for the first time,
Second batch mushroom flower bud is formed, and the life of mushroom flower bud is existing 20 days or so, and fructification is ripe, harvesting；About 60 days or so after adopting for the second time, the 3rd batch of mushroom
Flower bud is formed, and the life of mushroom flower bud is existing 20 days or so, and fructification is ripe, harvesting.Per batch after harvesting, fungus block surface is cleared up, earthing is mended again,
Place 7 days, after after bacterium life restoration ecosystem, management is equally repeated with early stage.First mushroom biological transformation ratio 7.1%, second batch mushroom
Biological transformation ratio 6.5%, the 3rd batch of mushroom biological transformation ratio 6.8% harvests three batches, the total biological transformation ratio 20.4% of Second Year altogether.
Biennial thing conversion ratio amounts to 52.5%.
The present invention of embodiment 2 is used for the cultivation of Black Ganoderma
Cultural method is with reference to embodiment 1.
As a result
(1) this time experiment in cultivation production tank of fermentation tank 11, pollution rate is 0；
(2) ganoderma lucidum bacterium bag covering Nutrition Soil 20 days or so, 50% bacterium bag mushroom flower bud, mushroom flower bud occur 25 days or so, fructification into
It is ripe, harvesting；About 60 days or so after adopting for the first time, second batch mushroom flower bud is formed, and mushroom flower bud occurs 25 days or so, and fructification is ripe, harvesting；
About 60 days or so after adopting for the second time, the 3rd batch of mushroom flower bud is formed, and mushroom flower bud occurs 25 days or so, and fructification is ripe, harvesting；.Per batch
After harvesting, fungus block surface is cleared up, earthing is mended again, is placed 7 days, after after bacterium life restoration ecosystem, management is equally repeated with early stage.The
A collection of mushroom biological transformation ratio 9.7%, the 3rd batch of mushroom biological transformation ratio 8.9%, the 3rd batch of mushroom biological transformation ratio 6.5%, altogether harvesting
Three batches, the total biological transformation ratio 25.1% of First Year.
After (3) the 3rd batches are adopted, bacterium bag earthing is survived the winter, to Second Year mid-April, recovers moisture content and air humidity pipe
Reason.Water management about 20 days or so, mushroom flower bud occurs, and after 25 days or so, fructification was ripe, harvesting；About 60 days after adopting for the first time
Left and right, second batch mushroom flower bud is formed, and mushroom flower bud occurs 25 days or so, and fructification is ripe, harvesting；About 60 days or so after adopting for the second time, the
Three batches of mushroom flower buds are formed, and mushroom flower bud occurs 25 days or so, and fructification is ripe, harvesting.Per batch after harvesting, fungus block surface is cleared up, again
Earthing is mended, is placed 7 days, after after bacterium life restoration ecosystem, management is equally repeated with early stage.First mushroom biological transformation ratio 9.3%, the
Two batches of mushroom biological transformation ratios 7.1%, the 3rd batch of mushroom biological transformation ratio 6.2% harvests three batches, the total biological transformation ratio of Second Year altogether
22.6%.
Biennial thing conversion ratio amounts to 47.7%.
The solid spawn of embodiment 3 compares with fluid present invention cultivating bacterial spawn
(1) using solid spawn inoculation with fluid present invention inoculation and culture medium to red ganoderma fruiting bag mycelial growth with
Output condition (the results are shown in Table 1)
The wooden fruiting bag cultivating formula (CK) of conventional section：Weedtree branch 80.0%, weed tree sawdust 12.0%, rice bran 6%, land plaster
1.0%th, calcium superphosphate 1.0%；
Fruiting bag culture medium cultivation formula C/D (be the same as Example 1) of the present invention：Culture medium D：Acacia rachii branch 100%, every bag
Additional 80g mycelia restoration ecosystem auxiliary compost (culture medium C).
Fruiting bag culture medium is carried out with reference to embodiment 1, and packs (bag specification 40cm × 60cm), sterilizing, is then connect respectively
Solid spawn and liquid spawn are planted, bacterium bag is cultivated in 25-28 DEG C of culturing room.
After bacterium bag mycelia is covered with, mycelia afterripening, earthing and management of producing mushroom are carried out by embodiment 1.
Influence of the conventional medium of table 1 with culture medium C of the present invention to red ganoderma fruiting bag mycelial growth is compared
The result of the test of table 1 is analyzed using poor (DMRT) method of the new multipoles of Deng Kenshi, formed objects lowercase alphabet after data
Show that difference is not notable (table 2,3,4,5 is same) in 1% and 5% level.Biological transformation ratio (%)=fructification fresh weight/bacterium bag in table
Compost dry weight × 100%.
As seen from Table 1：Under the wooden plastic bag cultivation Ways of fruiting of traditional section, the bacterium bag manufactured using fluid present invention strain,
Under between at the trial, its processing mycelial growth rate is substantially better than solid spawn and connects fruiting bag, but nothing both biological transformation ratio
Significant difference；Under acacia rachii branch plastic bag cultivation Ways of fruiting of the present invention, the bacterium bag manufactured using fluid present invention strain, in examination
Test under the time, it handles mycelial growth rate and is substantially better than the solid spawn that solid spawn connects fruiting bag and the wooden plastic bag cultivation of traditional section
Handled with liquid spawn, and biological transformation ratio highest in four processing.
Experiment shows that fruiting bag culture medium of the present invention is with the case of cooperation fluid present invention strain, shortening red ganoderma bacterium
Silk growth time in terms of improving yield with having larger advantage.
(2) using solid spawn inoculation with fluid present invention inoculation and culture medium to Black Ganoderma fruiting bag mycelial growth with
Output condition (the results are shown in Table 2)
The wooden fruiting bag cultivating formula (CK) of conventional section：Weedtree branch 80.0%, weed tree sawdust 12.0%, rice bran 6%, land plaster
1.0%th, calcium superphosphate 1.0%；
Fruiting bag culture medium cultivation formula C/D (be the same as Example 1) of the present invention：Culture medium D：Acacia rachii branch 100%, every bag
Additional 80g mycelia restoration ecosystem auxiliary compost (culture medium C).
Fruiting bag culture medium is carried out with reference to embodiment 1, and packs (bag specification 40cm × 60cm), sterilizing, is then connect respectively
Solid spawn and liquid spawn are planted, bacterium bag is cultivated in 25-28 DEG C of culturing room.
After bacterium bag mycelia is covered with, mycelia afterripening, earthing and management of producing mushroom are carried out by embodiment 1.
Influence of the conventional medium of table 2 with culture medium C of the present invention to Black Ganoderma fruiting bag mycelial growth is compared
The result of the test of table 1 is analyzed using poor (DMRT) method of the new multipoles of Deng Kenshi, formed objects lowercase alphabet after data
Show that difference is not notable in 1% and 5% level.Biological transformation ratio (%)=fructification fresh weight/cultivating in a fungus bag material dry weight in table ×
100%.
As seen from Table 2：Under the wooden plastic bag cultivation Ways of fruiting of traditional section, the bacterium bag manufactured using fluid present invention strain,
Under between at the trial, its processing mycelial growth rate is substantially better than solid spawn and connects fruiting bag, but nothing both biological transformation ratio
Significant difference；Under acacia rachii branch plastic bag cultivation Ways of fruiting of the present invention, the bacterium bag manufactured using fluid present invention strain, in examination
Test under the time, it handles mycelial growth rate and is substantially better than the solid spawn that solid spawn connects fruiting bag and the wooden plastic bag cultivation of traditional section
Handled with liquid spawn, and biological transformation ratio highest in four processing.
Experiment shows that fruiting bag culture medium of the present invention is with the case of cooperation fluid present invention strain, bacterium is shortened in Black Ganoderma
Silk growth time in terms of improving yield with having larger advantage.
The traditional zymotic tank of embodiment 4 improves compareing for fermentation tank cultivation with the present invention
As shown in figure 1, relative to traditional zymotic tank, two non-return valves are installed on the blast pipe of fermentation tank of the present invention, with
Prevent bacterium solution happens suddenly to have a power failure during fermenting from causing extraneous bad air to enter caused fermentation failure (pollution), the gas of fermentation tank
The gas tank of one Pyatyi filter is installed at pump, can prevent that burst power failure causes no gas supply to lead during bacterium solution fermentation
Enter caused fermentation failure and the necrosis of mycelium anoxic in extraneous bad air.
(1) when carrying out Liquid Culture with unmodified fermentation tank (CK) using improved fermentation tank of the invention, liquid spawn
Yield rate compares and (the results are shown in Table 3)
Culture medium A, B preparation, packing, sterilization, inoculation are carried out with reference to embodiment 1.
After fermentor liquid culture medium inoculated culture 24 hours, it was measured by sampling whether there is miscellaneous bacteria sense from sample tap every 12 hours
Contaminate (culture medium rubbing method).
Whether bacterium infection is adopted as phenol red meat soup fluid nutrient medium detection, and its Main Ingredients and Appearance is：Peptone 1%, grape
Sugar 0.3%, beef extract 0.3%, sodium chloride 0.5%, phenol red 0.003%.By institute's test sample product access culture medium, if any bacterium sense
Dye, then nutrient solution reddens；Whether mycotic infection is using the detection of meat soup solid medium, and its Main Ingredients and Appearance is：Peptone 1%, Portugal
Grape sugar 0.3%, beef extract 0.3%, sodium chloride 0.5%, agar powder 6.0g/L.Institute's test sample product are accessed into culture by streak inoculation method
Base, is cultivated 3 days at 30-32 DEG C, observes mycotic infection situation.
The present invention of table 3 repacking fermentation tank is not with reequiping bacterium solution yield rate contrast (the experiment kind that fermentation tank is cultivated：Red spirit
Sesame)
The present invention of table 4 repacking fermentation tank is not with reequiping bacterium solution yield rate contrast (the experiment kind that fermentation tank is cultivated：Black spirit
Sesame)
From table 3,4：The condition of continued power in the training period, original-pack fermentation tank improves fermentation tank life with the present invention
The bacterium solution of production is identical in quality, is detected without bacterium and mould.Under conditions of having a power failure 20 minutes after 48 hours of incubation, using this hair
The produced liquid spawn of bright fermentation detects without bacterium and mould, but red ganoderma is cultivating 120 with the original-pack fermentation tank of Black Ganoderma
After hour, all infection, and based on mycotic infection.This explanation, in the case of unexpected have a power failure, original-pack fermentation tank exhaust
There is outside air entrance pipe portion position, and the mycotic spore in air is brought in into nutrient solution, and fermentation tank of the present invention is in blast pipe
Place has added two non-return valves, so as to prevent the invasion of outage outside air.
Experiment shows that fermentation tank of the present invention has bright in terms of reply burst power failure institute introduced strains liquid inductance dye than original-pack fermentation tank
Aobvious advantage.
The conventional vaccination of embodiment 5 is compareed with the improved portable inoculation kettle of the present invention
As shown in Fig. 2 the portable inoculation kettle (about 30L) that the present invention is designed is main by being sequentially connected by anti-high pressure rubber pipe
Gas of heap of stone (containing pressure gauge), filter, stainless steel storage bacterium solution container, inoculating gun composition, storage bacterium solution container top sets dress liquid mouthful, side
Portion sets brace.
Usage：By stainless steel storage bacterium solution container, filter (0.2 μM of filter pore size), pressure gauge, inoculating gun, high pressure resistant
Sebific duct is put together to do and sterilized 20 minutes at a packaging, 120 DEG C, is cooled down stand-by；Using flame sealing method, pass through fermentation tank
Discharging tube, portable inoculation kettle is moved into by the liquid spawn fermented；Movable type inoculation kettle movement is to being put in the bacterium bag that disinfects
Transfer room, inoculation.
The portable inoculation kettle inoculation liquid spawn of the present invention of table 5 and Conventional solid strain inoculation efficiency and bacterium bag yield rate ratio
Compared with
As seen from Table 5：Liquid spawn inoculum bag is inoculated with using the portable kettle that is inoculated with of the invention, inoculation efficiency is significantly high
It is also obvious that the method being inoculated with is lengthened higher than fermentation tank discharging tube in using solid spawn, while bacterium bag pollution rate only has
0.6%, hence it is evident that less than other processing.Therefore, the raising life that the portable inoculation kettle of the present invention can be larger on inoculation liquid spawn
Efficiency is produced, and reduces bacterium bag pollution rate.
Claims (7)
1. the efficient culture medium of cultivation of glossy ganoderma, it is characterised in that including the culture medium A for liquid Mother culture and for fermenting
The culture medium B of tank strain cultivation；The culture medium A using potato, mushroom carpophore as Main Ingredients and Appearance, the culture medium B with
Soya bean, mushroom carpophore, ganoderma lucidum fruiting bag give up bacteria residue for Main Ingredients and Appearance.
2. the efficient culture medium of cultivation of glossy ganoderma, it is characterised in that including culture medium A, B, C, D, wherein：
The formula of culture medium A be per 1000ml nutrient solutions in given up bacteria residue containing potato 200.0g, rice bran 50.0g, ganoderma lucidum fruiting bag
50.0g, mushroom carpophore 10.0g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 1.5g, sucrose 25.0g, water 700-900ml are settled to
1000ml；
Culture medium B formula be per 1000ml nutrient solutions in given up bacteria residue containing potato 100.0g, rice bran 50.0g, ganoderma lucidum fruiting bag
50.0g, mushroom carpophore 40.0g, analysis for soybean powder 5.0g, potassium dihydrogen phosphate 6.0g, magnesium sulfate 2.5g, glucose 10.0g, fermentation food
Product defoamer 0.1ml, water 700-900ml, are settled to 1000ml；
The formula of culture medium C be per 1000g culture mediums in given up bacteria residue 370.0g, sweet containing cotton seed hull 400.0g, ganoderma lucidum fruiting bag
Bagasse 100.0g, corncob 100.0g, land plaster 10.0g, calcium superphosphate 10.0g, potassium dihydrogen phosphate 10.0g, culture medium add water
600-700ml；
Culture medium D formula be per 1000g culture mediums in contain acacia rachii branch 1000g, culture medium adds water 600-700ml.
3. the efficient culture medium of cultivation of glossy ganoderma according to claim 2, it is characterised in that：
The culture medium A is prepared by following operation：By potato, rice bran, ganoderma lucidum fruiting bag give up bacteria residue, this 4 kinds of mushroom carpophore into
Divide and mix, blend, add water 700ml, boil to boiling, maintain boiling 10 minutes, filtering is removed slag, stays filtered juice；By biphosphate
Potassium, magnesium sulfate, sucrose add filtrate, mix dissolving；Adding water makes final culture volume reach 1000ml；
The culture medium B is prepared by following operation：By potato, rice bran, ganoderma lucidum fruiting bag useless bacteria residue, mushroom carpophore, analysis for soybean powder
This 5 kinds of compositions, which mix, to be mixed, is boiled to boiling, filtering, remove slag reserved filtrate；By defoamer, potassium dihydrogen phosphate, magnesium sulfate, Portugal
Grape sugar, which is added in appropriate water, to be dissolved；Filtrate and the aqueous solution are mixed, and adding water makes final nutrient solution volume reach 1000ml；
The culture medium C is prepared by following operation：By cotton seed hull, ganoderma lucidum fruiting bag useless bacteria residue, bagasse, corncob, land plaster
This 5 kinds of compositions, which mix, to be mixed；Calcium superphosphate, potassium dihydrogen phosphate are dissolved in appropriate water；By mixture and the aqueous solution
Mixing, and adding water makes final moisture content in medium reach 65%；
The culture medium D is prepared by following operation：By acacia rachii branch wood parison water 12-15 hours to water-holding capacity up to 65%.
4. usage right requires that 2 culture mediums carry out the high efficiency method of cultivation of glossy ganoderma, it is characterised in that it is female that culture medium A is used for into liquid
Culture is planted, culture medium B is used for fermentor liquid Spawn incubation, culture medium C is entered to the liquid spawn of fruiting bag for rigid connection
Activated growth, is used for fruiting bag Mycelium culture by culture medium D.
5. the high efficiency method of cultivation of glossy ganoderma according to claim 4, it is characterised in that comprise the following steps：
(1) culture medium A is sub-packed in 1000ml vial, every bottle of nutrient solution 500ml, 120 DEG C sterilize 60 minutes, are trained after sterilizing
Foster base is cooled to room temperature, accesses the solid parent species of 1.0cm × 1.0cm × 1.0cm sizes, 25-28 DEG C of shaking table culture at room temperature, shakes
Bed shakes 150 revs/min of speed, and incubation time 5 days obtains the liquid parent species that mycelial density accounts for nutrient solution 80%；
(2) culture medium B is sub-packed in 450L fermentation tank, per canned nutrient solution 300L, 60 minutes at 120 DEG C, cultivated after sterilizing
Liquid is cooled to room temperature, accesses 1000ml liquid parent species, 28 DEG C are cultivated at room temperature, maintains tank pressure 0.03-0.05mPa, and gas builds pressure
Table 0.15mPa, incubation time 5-6 days obtains the liquid spawn that mycelium pellet density accounts for 80%；
(3) after fermentor liquid culture medium inoculated culture 24 hours, it was measured by sampling whether there is miscellaneous bacteria sense from sample tap every 12 hours
Dye；
(4) the culture medium D prepared is sub-packed in 40cm × 60cm polypropylene plastics pockets, per packed siccative 4000g, in culture medium
D surfaces add 80g culture medium C, surface of tiling, and tie sack, sterilization 240 minutes, then take out at 120 DEG C, cool down
It is stand-by to room temperature；Transfer room inoculation is moved into, every bag is accessed 80ml liquid spawns, and 28 DEG C are cultivated at room temperature, incubation time 60-65
My god, obtain covering with the fruiting bag of mycelia；
(5) bacterium bag for covering with mycelia is peelled off into polybag, is put in the good greenhouse of ground sterilization or sylvan life, in one layer of bacterium bag surface cover
Plastic film, room temperature is controlled at 25-28 DEG C, is maintained 5-7 days, and it is secondary with ventilation during which to raise film daily, raises every time
10 minutes time；
(6) the bacterium bag surface earthing of ripe effect in the completed, soil layer 2-3CM is thick, pours once permeable after earthing, later every 3-4
It drenches a water；
(7) 28-35 DEG C of mushroom house temperature, air humidity 80-90%, before the bacterium bag mushroom flower bud of covering Nutrition Soil is not unearthed, every 3-4
It drenches a water；After more than 50% bacterium bag has mushroom flower bud to be formed, drench within 3 days once permeable, until harvesting；Every batch adopt after stop spray
Water 5-7 days.
6. the high efficiency method of cultivation of glossy ganoderma according to claim 5, it is characterised in that：Pacify on the blast pipe of the fermentation tank
Equipped with two non-return valves, the gas tank of a Pyatyi filter is installed at the air pump of fermentation tank.
7. the high efficiency method of cultivation of glossy ganoderma according to claim 5, it is characterised in that inoculation uses portable in step (4)
Kettle is inoculated with, movable type inoculation kettle is mainly built by the gas being sequentially connected by anti-high pressure rubber pipe, filter, stainless steel storage bacterium solution are held
Device, inoculating gun composition, storage bacterium solution container top set dress liquid mouthful, sidepiece and set brace.
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