CN107455142A - A kind of implantation methods for improving ganoderma lucidum quality - Google Patents
A kind of implantation methods for improving ganoderma lucidum quality Download PDFInfo
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- CN107455142A CN107455142A CN201710753406.0A CN201710753406A CN107455142A CN 107455142 A CN107455142 A CN 107455142A CN 201710753406 A CN201710753406 A CN 201710753406A CN 107455142 A CN107455142 A CN 107455142A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The present invention relates to agricultural technology field, and in particular to a kind of implantation methods for improving ganoderma lucidum quality, including step in detail below：(1) selection of base material is cultivated：Culture medium includes 19 kinds of raw materials such as ganoderma lucidum leftover bits and pieces, soya bean bran, mung bean shell, the wood chip that deoils, wheat bran, jacket, Malus spectabilis complete stool, castor leaf, shrub althea flower, fluffy sub- Lay；(2) preparation of culture medium；(3) it is inoculated with；(4) training orientation；(5) harvest.It is an advantage of the invention that this method is simple and easy, there is great generalization, the culture medium energy Anti-bacterium of use, survival rate can be improved, reduce pest and disease damage Probability, improve the profit of cultivation of glossy ganoderma；And the amount containing selenium element in increase ganoderma lucidum, the quality of ganoderma lucidum is improved, there is higher economic value.
Description
【Technical field】
The present invention relates to agricultural technology field, and in particular to a kind of implantation methods for improving ganoderma lucidum quality.
【Background technology】
Ganoderma lucidum since ancient times it is among the people be exactly a kind of medicine-food two-purpose fungi, enjoy compatriots to favor, Wild ganoderma quantity is few,
The needs of people can not be met, ganoderma lucidum main source is still artificial cultivation at present.
Existing implantation methods are：Short section wood clinker cultivation of glossy ganoderma and for material cultivation of glossy ganoderma, its technological process is respectively：①
Selection seeds → felling → saw section → hoop bundle water transfer → pack → sterilizing → inoculation → culture (do ridge-up bed and take cool canopy) → de- bag →
Bury soil → manage out sesame (spore collection) → harvesting.2. select formula → spice → pack → sterilizing → inoculation → culture → management
Go out sesame (spore collection) → harvesting.It is primarily present problem：Cultivation of glossy ganoderma flow is cumbersome, and vast planting household is not easy to grasp, involved
The equipment arrived is a lot.Substantial amounts of material need to transport the wrapping sterilizing of sterilizing field back, be inoculated with easily pollution.Its method is adapted only to the strain of specialty
Manufacturing enterprise operates, and is not easy to promote.Therefore, select a kind of good culture medium for cultivating matter critically important, traditional cultivation matrix
Formula is：75% wood chip, 20% wheat bran, 3% corn flour, 1% land plaster and 1% precipitated calcium carbonate, also useful section of wood or log
To be cultivated instead of wood chip, these cultivation matrixes will all cause the waste of tree resources, and as a large amount of of ganoderma lucidum product open
Hair, and the intensification that people are recognized ganoderma lucidum, the demand of ganoderma lucidum is increasing, now, finds suitable cultivation matrix raw material
The problem of being paid close attention to as ganoderma lucidum planting industry.Because the matrix of ganoderma lucidum has parasite or other germs, cause the ganoderma lucidum after transplanting
The easy different classes of disease of strain infection, its living contaminants is higher, causes Ganoderma Lucidum mycelia to be disintegrated, and Nutrient medium is by broken
Bad, matrix is gone mouldy smelly when serious, and then further influences the production of its ganoderma lucidum.Therefore, needed in industrialized production
Want a kind of new raising ganoderma lucidum product that can suppress ganoderma lucidum matrix germ reproduction, reduce lucidum strain infection parasite or other germs
The implantation methods of matter.
【The content of the invention】
The goal of the invention of the present invention is：For it is above-mentioned the problem of, the present invention provide it is a kind of improve ganoderma lucidum quality plantation
Method, method is simple and easy, has great generalization, the culture medium energy Anti-bacterium of use, can improve survival rate, reduces disease pest
Evil Probability, improve the profit of cultivation of glossy ganoderma；And the amount containing selenium element in increase ganoderma lucidum, improve the quality of ganoderma lucidum, have compared with
High economic value.
To achieve these goals, the technical solution adopted by the present invention is as follows：
A kind of implantation methods for improving ganoderma lucidum quality, including step in detail below：
(1) selection of base material is cultivated：Culture medium weighs 10-25 parts by weight soya beans bran, 10-20 weight in parts by weight
Part is deoiled wood chip, 10-20 weight Ganoderma Lucidums leftover bits and pieces, 8-20 parts by weight wheat bran, 10-20 parts by weight mung bean shell, 5-15 parts by weight
Jacket, 5-15 parts by weight Malus spectabilis complete stool, 4-10 parts by weight castor leaf, 5-10 parts by weight shrub althea flower, the fluffy son of 4-10 parts by weight
Lay, 5-10 parts by weight purslane, 3-10 parts by weight common perilla leaf, 3-10 parts by weight sodium onitrophenol, the Guang of 4-10 parts by weight selenium half
Propylhomoserin, 5-10 parts by weight sodium selenite, 3-10 parts by weight of lemon, 3-10 parts by weight polygonum flaccidums grass juice factor, 5-10 parts by weight garlic juice, 5-
10 parts by weight composite microbial bacterias；
(2) preparation of culture medium：
A, in parts by weight, purslane, common perilla leaf, castor leaf, lemon is taken to be extracted to obtain common perilla leaf respectively and carry
Take liquid, castor-oil plant leaf extract, lemon chromocor extract；
B, in parts by weight, weigh ganoderma lucidum leftover bits and pieces, garlic juice, mung bean shell, wheat bran, Malus spectabilis complete stool, polygonum flaccidum grass juice factor,
Soya bean bran, jacket, the wood chip that deoils, shrub althea flower, fluffy sub- Lay, composite microbial bacteria, sodium onitrophenol sodium selenite, selenium
Cysteine obtains base-material after building heap and fermenting 7-12 days；
C, and then by step A common perilla leaf extract, castor-oil plant leaf extract, lemon chromocor extract and the step B's obtained
Base-material mixing is follow-up to continue heap 12-24 hours, and controls water content to can obtain culture medium clinker in 40-45%；
(3) it is inoculated with：The culture medium clinker that step (2) obtains is fed in kind of pocket, then carries out sterilization treatment, then connect
And aseptic inoculation is carried out in desinfection chamber, Ganoderma Lucidum is inoculated into kind of pocket；
(4) training orientation：Kind pocket after inoculation is stacked to fungus shelf, indoor temperature control is in 22-26 DEG C of progress
Walk bacterium 20 days, light reaches 600-700LX in regulation culture room afterwards, and at 25-30 DEG C, relative humidity control exists control indoor temperature
Between 80-85%, indoor holding is dark；
(5) harvest：When ganoderma lucidum cap deploys, cap starts keratin, starts to eject spore, you can harvesting.
Further illustrate, it is preferable that the culture medium is that following raw material is prepared：13-20 parts by weight soya beans bran,
12-18 parts by weight deoil wood chip, 12-17 weight Ganoderma Lucidums leftover bits and pieces, 10-17 parts by weight wheat bran, 13-17 parts by weight mung bean shell,
8-12 parts by weight jacket, 8-13 parts by weight Malus spectabilis complete stool, 5-8 parts by weight castor leaf, 6-9 parts by weight shrub althea flower, 6-9 weight
The fluffy sub- Lay of part, 6-9 parts by weight purslane, 5-9 parts by weight common perilla leaf, 5-9 parts by weight sodium onitrophenol, 5-9 parts by weight selenium half
Cystine, 6-9 parts by weight sodium selenite, 5-9 parts by weight of lemon, 5-9 parts by weight polygonum flaccidums grass juice factor, 6-9 parts by weight garlic juice, 6-9
Parts by weight composite microbial bacteria.
It is prepared it is further preferred that the culture medium is following raw material：16 parts by weight soya bean brans, 14 parts by weight are deoiled
Wood chip, 15 weight Ganoderma Lucidum leftover bits and pieces, 14 parts by weight wheat bran, 15 parts by weight mung bean shells, 10 parts by weight jackets, 10 parts by weight
Malus spectabilis complete stool, 7 parts by weight castor leaves, 7 parts by weight shrub althea flowers, the fluffy sub- Lay of 8 parts by weight, 8 parts by weight purslanes, 7 parts by weight common perillas
Leaf, 7 parts by weight sodium onitrophenols, 6 parts by weight selenocysteins, 7 parts by weight sodium selenites, 6 parts by weight of lemon, 7 parts by weight
Polygonum flaccidum grass juice factor, 8 parts by weight garlic juices, 7 parts by weight composite microbial bacterias.
Further illustrate, the raw material that the composite microbial bacteria includes following parts by weight is prepared into：Se-enriched yeast bacterium solution
5-10 parts, the double spore bacterium bacterium solution 5-10 parts of high temperature, Bacillus alcaligenes bacterium solution 5-10 parts, nitrous acid coccus bacterium solution 5-12 parts, the brown fixed nitrogen of circle
Bacterium bacterium solution 5-10 parts, the double spore bacterium bacterium solution 4-12 parts of high temperature.
Further illustrate, it is preferable that the raw material that the composite microbial bacteria includes following parts by weight is prepared into：Selenium-rich ferment
Double 8 parts of the spore bacterium bacterium solutions of female 7 parts of bacterium solution, high temperature, 6 parts of Bacillus alcaligenes bacterium solution, 8 parts of nitrous acid coccus bacterium solution, azotobacter chroococcum bacterium solution 7
Part, double 9 parts of the spore bacterium bacterium solutions of high temperature.
Further illustrate, effective bacteria containing amount of the Se-enriched yeast bacterium solution in the composite microbial bacteria is 3.12 × 108-
8.56×108Effective bacteria containing amount of the double spore bacterium bacterium solutions of individual/mL, high temperature is 0.15 × 109-2.55×109Individual/mL, Bacillus alcaligenes bacterium
Effective bacteria containing amount of liquid is 1.25 × 108-5.65×108Individual/mL, effective bacteria containing amount of nitrous acid coccus bacterium solution for 1.24 ×
108-4.59×108Individual/mL, effective bacteria containing amount of azotobacter chroococcum bacterium solution are 5.26 × 108-8.54×108Individual/mL, high temperature are double
Effective bacteria containing amount of spore bacterium bacterium solution is 1.04 × 109-2.12×109Individual/mL.
Further illustrate, the concrete operations of the purslane extract are：Purslane is taken by water extraction and then obtains content
Reach the extract solution of 50% linoleic acid composition.
Further illustrate, the concrete operations of the common perilla leaf extract are：Common perilla leaf is taken to be crushed after being dried, by following
Ring ultrasonic extraction device is 1 according to volume ratio according to comminuted powder and solvent for extraction:2 ratio mixing extraction, Extracting temperature 50
Degree Celsius, supersonic frequency 50KHz, extract 2 minutes, circulation extraction 5 times, 5 liquid are merged after filtering, liquid loads solvent and returned
Receive 50 degrees Celsius of recycling designs of enrichment facility, then pressure be 10MPa, temperature is 30 DEG C, CO2Flow 15kg/h, time is
15 minutes, refined filtration, common perilla leaf extract, linoleic acid 35.4%, leukotrienes 56.5% are contained in common perilla leaf extract.
Further illustrate, the concrete operations of the castor-oil plant leaf extract are：Castor leaf is taken to be taken off by broken, soxhlet extraction
Fat, water extract-alcohol precipitation, centrifugation, water-bath are volatilized, are freeze-dried, and produce castor-oil plant leaf extract.
Further illustrate, the concrete operations of the lemon chromocor extract are：Take lemon carry out after peeling remove seed plus water and
Cellulase, volume ratio 1:1:0.02, digested, then after carrying out enzyme deactivation, enzymolysis liquid is obtained, the pH of enzymolysis liquid is adjusted
To after 4, it is extracted with ethyl acetate, takes organic phase, the 1wt.% of weight such as addition sodium hydrate aqueous solution in organic phase,
Stir, extracted, phase of fetching water, aqueous phase feeding milipore filter is filtered, then the penetrating fluid NF membrane by milipore filter
Concentrated, then after being dried, obtain lemon chromocor extract.
In summary, by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
The additional Guang of selenium half of substantial amounts of selenium element is enriched with the ganoderma lucidum leftover bits and pieces of the application, mung bean shell, soya bean bran, garlic juice
Selenium element is carried in propylhomoserin, sodium selenite urges rotten fermenting raw materials further to discharge the selenium element for decompositing and in other raw materials again,
So that in culture medium can rich selenium content be 0.145 (ω B)/10-6More than, so as to reach the Absorption of Ganoderma lucidum micro member containing selenium turned out
Element；A great number of elements, micro is discharged after everfermentation decomposes by wood chip, wheat bran, mung bean shell, soya bean bran, mung bean shell, jacket
Element, which is more easy to be eaten bacteria growing, to be absorbed, and substantial amounts of element, trace element, particularly selenium can be absorbed during edible fungi growth
Element, reach the effect of selenium-rich；Substantial amounts of selenium element is rich in the culture medium of the application, and passes through secondary fermentation, can not only
The more micro- organic matters of fermentation release, amino acid, can also be added by the processing of several raw materials linoleic acid in fertilizer,
Leukotrienes, Flavonoid substances content, by plant absorption, improve the content of the various organic matters in ganoderma lucidum；It is additional complete with Malus spectabilis
Strain, shrub althea flower, fluffy sub- Lay discharge alkaloid, phenols, ketone, performance oil, organic acid, eucalyptol, tannin, soaping agents after making compost
After being absorbed by plants, because each material is different in the amount that the position of plant is assembled, in the material of several substance releases
Also it can be liked according to edible mushroom and be absorbed to different positions, either on handle or mycelium, they absorb for the fructification of edible mushroom
Material be also what is differed, in order that each position of edible mushroom all absorbs different element according to hobby, enrichment, carries
The ganoderma lucidum quality of high ganoderma lucidum；And different defect phenomenons can occur in order to reduce the different parts of edible mushroom, so the applicant
Can have certain antibacterial itself, the ability of disease resistance to set out to reach each position of edible mushroom, by countless examinations
Orthogonal research is tested, polygonum flaccidum grass juice factor, garlic juice have the function that to suppress to harmful flora, and carrying out resisting based on garlic juice has
Outside evil flora, polygonum flaccidum grass juice factor auxiliary carries out anti-pernicious bacteria, and polygonum flaccidum grass juice factor collaboration phenols, ketone, play oil, organic acid,
Tannin, soaping agents are eaten bacterium and absorbed faster；Phenols, ketone, play oil, organic acid, tannin, soaping agents quilt faster
Edible mushroom composite microbial bacteria also has the ability of disease and insect resistance, finds that each material can resist different diseases by research
Evil, the formation for suppressing different population, the contact between them are complemented each other, and reach the ability for improving sterilization, murdering worm, therefore,
The phenomenon for disease occur is reduced, improves ganoderma lucidum yield, peasant's cost is reduced and increases economic efficiency；Additional sodium selenite can promote
Each trace element in fermentation release constitutive material, improves the organic matter content in culture medium.
【Embodiment】
In order that the technical means, the inventive features, the objects and the advantages of the present invention are easy to understand, tie below
Embodiment is closed, the present invention is expanded on further.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention,
It is not intended to limit the present invention.
Embodiment 1：
A kind of implantation methods for improving ganoderma lucidum quality, including step in detail below：
(1) selection of base material is cultivated：Culture medium weighs 10 parts by weight soya bean brans in parts by weight, 10 parts by weight are deoiled
Wood chip, 10 weight Ganoderma Lucidum leftover bits and pieces, 8 parts by weight wheat bran, 10 parts by weight mung bean shells, 5 parts by weight jackets, 5 parts by weight sea
Chinese bush cherry complete stool, 4 parts by weight castor leaves, 5 parts by weight shrub althea flowers, the fluffy sub- Lay of 4 parts by weight, 5 parts by weight purslanes, 3 parts by weight common perilla leafs,
3 parts by weight sodium onitrophenols, 4 parts by weight selenocysteins, 5 parts by weight sodium selenites, 3 parts by weight of lemon, 3 parts by weight polygonum flaccidums
Grass juice factor, 5 parts by weight garlic juices, 5 parts by weight composite microbial bacterias；
Wherein, raw material of the composite microbial bacteria including following parts by weight is prepared into：5 parts of Se-enriched yeast bacterium solution, high temperature are double
The double spore bacterium bacterium of 5 parts of spore bacterium bacterium solution, 5 parts of Bacillus alcaligenes bacterium solution, 5 parts of nitrous acid coccus bacterium solution, 5 parts of azotobacter chroococcum bacterium solution, high temperature
4 parts of liquid；
Wherein, effective bacteria containing amount of the Se-enriched yeast bacterium solution in composite microbial bacteria is 3.12 × 108Individual/mL, high temperature are double
Effective bacteria containing amount of spore bacterium bacterium solution is 0.15 × 109Individual/mL, effective bacteria containing amount of Bacillus alcaligenes bacterium solution are 1.25 × 108Individual/mL,
Effective bacteria containing amount of nitrous acid coccus bacterium solution is 1.24 × 108Individual/mL, effective bacteria containing amount of azotobacter chroococcum bacterium solution for 5.26 ×
108Effective bacteria containing amount of the double spore bacterium bacterium solutions of individual/mL, high temperature is 1.04 × 109Individual/mL.
(2) preparation of culture medium：
A, in parts by weight, purslane, common perilla leaf, castor leaf, lemon, sodium onitrophenol is taken to be extracted respectively
Obtain common perilla leaf extract, castor-oil plant leaf extract, lemon chromocor extract；
A, the concrete operations of purslane extract are：Taking purslane, then obtaining content reaches 50% linoleic acid by water extraction
The extract solution of composition；
B, the concrete operations of common perilla leaf extract are：Take common perilla leaf to be crushed after being dried, extracted and filled by circulating ultrasonic
It is 1 to put according to comminuted powder and solvent for extraction according to volume ratio:2 ratio mixing extraction, 50 degrees Celsius of Extracting temperature, supersonic frequency
Rate is 50KHz, is extracted 2 minutes, circulation extraction 5 times, and 5 liquid are merged after filtering, and liquid loads solvent recovery enrichment facility 50
Degree Celsius recycling design, then pressure be 10MPa, temperature is 30 DEG C, CO2Flow 15kg/h, the time be 15 minutes, refined filtration,
Common perilla leaf extract is obtained, contains linoleic acid 35.4%, leukotrienes 56.5% in common perilla leaf extract；
C, the concrete operations of castor-oil plant leaf extract are：Take castor leaf by broken, soxhlet extraction degreasing, water extract-alcohol precipitation, from
The heart, water-bath are volatilized, are freeze-dried, and produce castor-oil plant leaf extract；
D, the concrete operations of lemon chromocor extract are：Lemon is taken to add water and cellulase, volume after carrying out peeling remove seed
Than for 1:1:0.02, digested, then after carrying out enzyme deactivation, obtain enzymolysis liquid, the pH of enzymolysis liquid is adjusted to after 4, uses second
Acetoacetic ester extracts, and takes organic phase, and the 1wt.% of weight such as addition sodium hydrate aqueous solution, stirs, enter in organic phase
Row extraction, phase of fetching water, aqueous phase feeding milipore filter is filtered, then the penetrating fluid of milipore filter is concentrated with NF membrane, then
After being dried, lemon chromocor extract is obtained；
B, in parts by weight, weigh ganoderma lucidum leftover bits and pieces, garlic juice, mung bean shell, wheat bran, Malus spectabilis complete stool, polygonum flaccidum grass juice factor,
Soya bean bran, jacket, the wood chip that deoils, shrub althea flower, fluffy sub- Lay, composite microbial bacteria, sodium onitrophenol, sodium selenite, selenium
Cysteine obtains base-material after building heap and fermenting 7 days；
C, and then by step A common perilla leaf extract, castor-oil plant leaf extract, lemon chromocor extract and the step B's obtained
Base-material mixing is follow-up to continue heap 12 hours, and controls water content to can obtain culture medium clinker 40%；
(3) it is inoculated with：The culture medium clinker that step (2) obtains is fed in kind of pocket, then carries out sterilization treatment, then connect
And aseptic inoculation is carried out in desinfection chamber, Ganoderma Lucidum is inoculated into kind of pocket；
(4) training orientation：Kind pocket after inoculation is stacked to fungus shelf, indoor temperature control enters the bacterium that walks at 26 DEG C
20 days, light reached 600LX in regulation culture room afterwards, and control indoor temperature is at 25 DEG C, and relative humidity control is 80%, indoor guarantor
Hold dark；
(5) harvest：When ganoderma lucidum cap deploys, cap starts keratin, starts to eject spore, you can harvesting.
Embodiment 2：
A kind of implantation methods for improving ganoderma lucidum quality, including step in detail below：
(1) selection of base material is cultivated：Culture medium weighs 25 parts by weight soya bean brans in parts by weight, 20 parts by weight are deoiled
Wood chip, 20 weight Ganoderma Lucidum leftover bits and pieces, 20 parts by weight wheat bran, 20 parts by weight mung bean shells, 15 parts by weight jackets, 15 parts by weight
Malus spectabilis complete stool, 10 parts by weight castor leaves, 10 parts by weight shrub althea flowers, the fluffy sub- Lay of 10 parts by weight, 10 parts by weight purslanes, 10 parts by weight
Common perilla leaf, 10 parts by weight sodium onitrophenols, 10 parts by weight selenocysteins, 10 parts by weight sodium selenites, 10 parts by weight of lemon,
10 parts by weight polygonum flaccidum grass juice factors, 10 parts by weight garlic juices, 10 parts by weight composite microbial bacterias；
Wherein, raw material of the composite microbial bacteria including following parts by weight is prepared into：10 parts of Se-enriched yeast bacterium solution, high temperature are double
The double spores of 10 parts of spore bacterium bacterium solution, 10 parts of Bacillus alcaligenes bacterium solution, 12 parts of nitrous acid coccus bacterium solution, 10 parts of azotobacter chroococcum bacterium solution, high temperature
12 parts of bacterium bacterium solution；
Wherein, effective bacteria containing amount of the Se-enriched yeast bacterium solution in composite microbial bacteria is 8.56 × 108Individual/mL, high temperature are double
Effective bacteria containing amount of spore bacterium bacterium solution is 2.55 × 109Individual/mL, effective bacteria containing amount of Bacillus alcaligenes bacterium solution are 5.65 × 108Individual/mL,
Effective bacteria containing amount of nitrous acid coccus bacterium solution is 4.59 × 108Individual/mL, effective bacteria containing amount of azotobacter chroococcum bacterium solution for 8.54 ×
108Effective bacteria containing amount of the double spore bacterium bacterium solutions of individual/mL, high temperature is 2.12 × 109Individual/mL.
(2) preparation of culture medium：
A, in parts by weight, purslane, common perilla leaf, castor leaf, lemon, sodium onitrophenol is taken to be extracted respectively
Obtain common perilla leaf extract, castor-oil plant leaf extract, lemon chromocor extract；
A, the concrete operations of purslane extract are：Taking purslane, then obtaining content reaches 50% linoleic acid by water extraction
The extract solution of composition；
B, the concrete operations of common perilla leaf extract are：Take common perilla leaf to be crushed after being dried, extracted and filled by circulating ultrasonic
It is 1 to put according to comminuted powder and solvent for extraction according to volume ratio:2 ratio mixing extraction, 50 degrees Celsius of Extracting temperature, supersonic frequency
Rate is 50KHz, is extracted 2 minutes, circulation extraction 5 times, and 5 liquid are merged after filtering, and liquid loads solvent recovery enrichment facility 50
Degree Celsius recycling design, then pressure be 10MPa, temperature is 30 DEG C, CO2Flow 15kg/h, the time be 15 minutes, refined filtration,
Common perilla leaf extract is obtained, contains linoleic acid 35.4%, leukotrienes 56.5% in common perilla leaf extract；
C, the concrete operations of castor-oil plant leaf extract are：Take castor leaf by broken, soxhlet extraction degreasing, water extract-alcohol precipitation, from
The heart, water-bath are volatilized, are freeze-dried, and produce castor-oil plant leaf extract；
D, the concrete operations of lemon chromocor extract are：Lemon is taken to add water and cellulase, volume after carrying out peeling remove seed
Than for 1:1:0.02, digested, then after carrying out enzyme deactivation, obtain enzymolysis liquid, the pH of enzymolysis liquid is adjusted to after 4, uses second
Acetoacetic ester extracts, and takes organic phase, and the 1wt.% of weight such as addition sodium hydrate aqueous solution, stirs, enter in organic phase
Row extraction, phase of fetching water, aqueous phase feeding milipore filter is filtered, then the penetrating fluid of milipore filter is concentrated with NF membrane, then
After being dried, lemon chromocor extract is obtained；
B, in parts by weight, weigh ganoderma lucidum leftover bits and pieces, garlic juice, mung bean shell, wheat bran, Malus spectabilis complete stool, polygonum flaccidum grass juice factor,
Soya bean bran, jacket, the wood chip that deoils, shrub althea flower, fluffy sub- Lay, composite microbial bacteria, sodium onitrophenol sodium selenite, selenium
Cysteine obtains base-material after building heap and fermenting 12 days；
C, and then by step A common perilla leaf extract, castor-oil plant leaf extract, lemon chromocor extract and the step B's obtained
Base-material mixing is follow-up to continue heap 24 hours, and controls water content to can obtain culture medium clinker 45%；
(3) it is inoculated with：The culture medium clinker that step (2) obtains is fed in kind of pocket, then carries out sterilization treatment, then connect
And aseptic inoculation is carried out in desinfection chamber, Ganoderma Lucidum is inoculated into kind of pocket；
(4) training orientation：Kind pocket after inoculation is stacked to fungus shelf, indoor temperature control enters the bacterium that walks at 30 DEG C
20 days, light reached 700LX in regulation culture room afterwards, and control indoor temperature is at 30 DEG C, and relative humidity control is 85%, indoor guarantor
Hold dark；
(5) harvest：When ganoderma lucidum cap deploys, cap starts keratin, starts to eject spore, you can harvesting.
Embodiment 3：
A kind of implantation methods for improving ganoderma lucidum quality, including step in detail below：
(1) selection of base material is cultivated：Culture medium weighs 13 parts by weight soya bean brans in parts by weight, 12 parts by weight are deoiled
Wood chip, 12 weight Ganoderma Lucidum leftover bits and pieces, 10 parts by weight wheat bran, 13 parts by weight mung bean shells, 8 parts by weight jackets, 8 parts by weight sea
Chinese bush cherry complete stool, 5 parts by weight castor leaves, 6 parts by weight shrub althea flowers, the fluffy sub- Lay of 6 parts by weight, 6 parts by weight purslanes, 5 parts by weight common perilla leafs,
5 parts by weight sodium onitrophenols, 5 parts by weight selenocysteins, 6 parts by weight sodium selenites, 5 parts by weight of lemon, 5 parts by weight polygonum flaccidums
Grass juice factor, 6 parts by weight garlic juices, 6 parts by weight composite microbial bacterias；
Wherein, raw material of the composite microbial bacteria including following parts by weight is prepared into：7 parts of Se-enriched yeast bacterium solution, high temperature are double
The double spore bacterium bacterium of 8 parts of spore bacterium bacterium solution, 6 parts of Bacillus alcaligenes bacterium solution, 8 parts of nitrous acid coccus bacterium solution, 7 parts of azotobacter chroococcum bacterium solution, high temperature
9 parts of liquid；
Wherein, effective bacteria containing amount of the Se-enriched yeast bacterium solution in composite microbial bacteria is 4.55 × 108Individual/mL, high temperature are double
Effective bacteria containing amount of spore bacterium bacterium solution is 0.84 × 109Individual/mL, effective bacteria containing amount of Bacillus alcaligenes bacterium solution are 2.35 × 108Individual/mL,
Effective bacteria containing amount of nitrous acid coccus bacterium solution is 1.89 × 108Individual/mL, effective bacteria containing amount of azotobacter chroococcum bacterium solution for 5.98 ×
108Effective bacteria containing amount of the double spore bacterium bacterium solutions of individual/mL, high temperature is 1.28 × 109Individual/mL；
(2) preparation of culture medium：
A, in parts by weight, purslane, common perilla leaf, castor leaf, lemon, sodium onitrophenol is taken to be extracted respectively
Obtain common perilla leaf extract, castor-oil plant leaf extract, lemon chromocor extract；
A, the concrete operations of purslane extract are：Taking purslane, then obtaining content reaches 50% linoleic acid by water extraction
The extract solution of composition；
B, the concrete operations of common perilla leaf extract are：Take common perilla leaf to be crushed after being dried, extracted and filled by circulating ultrasonic
It is 1 to put according to comminuted powder and solvent for extraction according to volume ratio:2 ratio mixing extraction, 50 degrees Celsius of Extracting temperature, supersonic frequency
Rate is 50KHz, is extracted 2 minutes, circulation extraction 5 times, and 5 liquid are merged after filtering, and liquid loads solvent recovery enrichment facility 50
Degree Celsius recycling design, then pressure be 10MPa, temperature is 30 DEG C, CO2Flow 15kg/h, the time be 15 minutes, refined filtration,
Common perilla leaf extract is obtained, contains linoleic acid 35.4%, leukotrienes 56.5% in common perilla leaf extract；
C, the concrete operations of castor-oil plant leaf extract are：Take castor leaf by broken, soxhlet extraction degreasing, water extract-alcohol precipitation, from
The heart, water-bath are volatilized, are freeze-dried, and produce castor-oil plant leaf extract；
D, the concrete operations of lemon chromocor extract are：Lemon is taken to add water and cellulase, volume after carrying out peeling remove seed
Than for 1:1:0.02, digested, then after carrying out enzyme deactivation, obtain enzymolysis liquid, the pH of enzymolysis liquid is adjusted to after 4, uses second
Acetoacetic ester extracts, and takes organic phase, and the 1wt.% of weight such as addition sodium hydrate aqueous solution, stirs, enter in organic phase
Row extraction, phase of fetching water, aqueous phase feeding milipore filter is filtered, then the penetrating fluid of milipore filter is concentrated with NF membrane, then
After being dried, lemon chromocor extract is obtained；
B, in parts by weight, weigh ganoderma lucidum leftover bits and pieces, garlic juice, mung bean shell, wheat bran, Malus spectabilis complete stool, polygonum flaccidum grass juice factor,
Soya bean bran, jacket, the wood chip that deoils, shrub althea flower, fluffy sub- Lay, composite microbial bacteria, sodium onitrophenol, sodium selenite, selenium
Cysteine obtains base-material after building heap and fermenting 9 days；
C, and then by step A common perilla leaf extract, castor-oil plant leaf extract, lemon chromocor extract and the step B's obtained
Base-material mixing is follow-up to continue heap 15 hours, and controls water content to can obtain culture medium clinker 42%；
(3) it is inoculated with：The culture medium clinker that step (2) obtains is fed in kind of pocket, then carries out sterilization treatment, then connect
And aseptic inoculation is carried out in desinfection chamber, Ganoderma Lucidum is inoculated into kind of pocket；
(4) training orientation：Kind pocket after inoculation is stacked to fungus shelf, indoor temperature control enters the bacterium that walks at 27 DEG C
20 days, light reached 620LX in regulation culture room afterwards, and control indoor temperature is at 26 DEG C, and relative humidity control is 82%, indoor guarantor
Hold dark；
(5) harvest：When ganoderma lucidum cap deploys, cap starts keratin, starts to eject spore, you can harvesting.
Embodiment 4：
A kind of implantation methods for improving ganoderma lucidum quality, including step in detail below：
(1) selection of base material is cultivated：Culture medium weighs 20 parts by weight soya bean brans in parts by weight, 18 parts by weight are deoiled
Wood chip, 17 weight Ganoderma Lucidum leftover bits and pieces, 17 parts by weight wheat bran, 17 parts by weight mung bean shells, 12 parts by weight jackets, 13 parts by weight
Malus spectabilis complete stool, 8 parts by weight castor leaves, 9 parts by weight shrub althea flowers, the fluffy sub- Lay of 9 parts by weight, 9 parts by weight purslanes, 9 parts by weight common perillas
Leaf, 9 parts by weight sodium onitrophenols, 9 parts by weight selenocysteins, 9 parts by weight sodium selenites, 9 parts by weight of lemon, 9 parts by weight
Polygonum flaccidum grass juice factor, 9 parts by weight garlic juices, 9 parts by weight composite microbial bacterias；
Wherein, composite microbial bacteria includes double 6 parts of the spore bacterium bacterium solutions of 6 parts of the Se-enriched yeast bacterium solution of parts by weight, high temperature, production alkali bar
Double 6 parts of the spore bacterium bacterium solutions of 6 parts of bacterium bacterium solution, 7 parts of nitrous acid coccus bacterium solution, 6 parts of azotobacter chroococcum bacterium solution, high temperature；
Effective bacteria containing amount of Se-enriched yeast bacterium solution in composite microbial bacteria is 5.34 × 108The double spore bacterium bacterium of individual/mL, high temperature
Effective bacteria containing amount of liquid is 1.26 × 109Individual/mL, effective bacteria containing amount of Bacillus alcaligenes bacterium solution are 3.54 × 108Individual/mL, nitrous acid
Effective bacteria containing amount of coccus bacterium solution is 3.02 × 108Individual/mL, effective bacteria containing amount of azotobacter chroococcum bacterium solution are 7.10 × 108Individual/
Effective bacteria containing amount of the double spore bacterium bacterium solutions of mL, high temperature is 1.86 × 109Individual/mL；
(2) preparation of culture medium：
A, in parts by weight, purslane, common perilla leaf, castor leaf, lemon, sodium onitrophenol is taken to be extracted respectively
Obtain common perilla leaf extract, castor-oil plant leaf extract, lemon chromocor extract；
A, the concrete operations of purslane extract are：Taking purslane, then obtaining content reaches 50% linoleic acid by water extraction
The extract solution of composition；
B, the concrete operations of common perilla leaf extract are：Take common perilla leaf to be crushed after being dried, extracted and filled by circulating ultrasonic
It is 1 to put according to comminuted powder and solvent for extraction according to volume ratio:2 ratio mixing extraction, 50 degrees Celsius of Extracting temperature, supersonic frequency
Rate is 50KHz, is extracted 2 minutes, circulation extraction 5 times, and 5 liquid are merged after filtering, and liquid loads solvent recovery enrichment facility 50
Degree Celsius recycling design, then pressure be 10MPa, temperature is 30 DEG C, CO2Flow 15kg/h, the time be 15 minutes, refined filtration,
Common perilla leaf extract is obtained, contains linoleic acid 35.4%, leukotrienes 56.5% in common perilla leaf extract；
C, the concrete operations of castor-oil plant leaf extract are：Take castor leaf by broken, soxhlet extraction degreasing, water extract-alcohol precipitation, from
The heart, water-bath are volatilized, are freeze-dried, and produce castor-oil plant leaf extract；
D, the concrete operations of lemon chromocor extract are：Lemon is taken to add water and cellulase, volume after carrying out peeling remove seed
Than for 1:1:0.02, digested, then after carrying out enzyme deactivation, obtain enzymolysis liquid, the pH of enzymolysis liquid is adjusted to after 4, uses second
Acetoacetic ester extracts, and takes organic phase, and the 1wt.% of weight such as addition sodium hydrate aqueous solution, stirs, enter in organic phase
Row extraction, phase of fetching water, aqueous phase feeding milipore filter is filtered, then the penetrating fluid of milipore filter is concentrated with NF membrane, then
After being dried, lemon chromocor extract is obtained；
B, in parts by weight, weigh ganoderma lucidum leftover bits and pieces, garlic juice, mung bean shell, wheat bran, Malus spectabilis complete stool, polygonum flaccidum grass juice factor,
Soya bean bran, jacket, the wood chip that deoils, shrub althea flower, fluffy sub- Lay, composite microbial bacteria, sodium onitrophenol, sodium selenite, selenium
Cysteine obtains base-material after building heap and fermenting 10 days；
C, and then by step A common perilla leaf extract, castor-oil plant leaf extract, lemon chromocor extract and the step B's obtained
Base-material mixing is follow-up to continue heap 18 hours, and controls water content to can obtain culture medium clinker 43%；
(3) it is inoculated with：The culture medium clinker that step (2) obtains is fed in kind of pocket, then carries out sterilization treatment, then connect
And aseptic inoculation is carried out in desinfection chamber, Ganoderma Lucidum is inoculated into kind of pocket；
(4) training orientation：Kind pocket after inoculation is stacked to fungus shelf, indoor temperature control enters the bacterium that walks at 28 DEG C
20 days, light reached 650LX in regulation culture room afterwards, and control indoor temperature is at 27 DEG C, and relative humidity control is 83%, indoor guarantor
Hold dark；
(5) harvest：When ganoderma lucidum cap deploys, cap starts keratin, starts to eject spore, you can harvesting.
Embodiment 5：
A kind of implantation methods for improving ganoderma lucidum quality, including step in detail below：
(1) selection of base material is cultivated：Culture medium weighs 16 parts by weight soya bean brans in parts by weight, 14 parts by weight are deoiled
Wood chip, 15 weight Ganoderma Lucidum leftover bits and pieces, 14 parts by weight wheat bran, 15 parts by weight mung bean shells, 10 parts by weight jackets, 10 parts by weight
Malus spectabilis complete stool, 7 parts by weight castor leaves, 7 parts by weight shrub althea flowers, the fluffy sub- Lay of 8 parts by weight, 8 parts by weight purslanes, 7 parts by weight common perillas
Leaf, 7 parts by weight sodium onitrophenols, 6 parts by weight selenocysteins, 7 parts by weight sodium selenites, 6 parts by weight of lemon, 7 parts by weight
Polygonum flaccidum grass juice factor, 8 parts by weight garlic juices, 7 parts by weight composite microbial bacterias；
Wherein, raw material of the composite microbial bacteria including following parts by weight is prepared into：9 parts of Se-enriched yeast bacterium solution, height
Warm double 9 parts of spore bacterium bacterium solutions, 8 parts of Bacillus alcaligenes bacterium solution, 11 parts of nitrous acid coccus bacterium solution, 9 parts of azotobacter chroococcum bacterium solution, the double spores of high temperature
10 parts of bacterium bacterium solution；
Wherein, effective bacteria containing amount of the Se-enriched yeast bacterium solution in composite microbial bacteria is 7.26 × 108Individual/mL, high temperature are double
Effective bacteria containing amount of spore bacterium bacterium solution is 1.84 × 109Individual/mL, effective bacteria containing amount of Bacillus alcaligenes bacterium solution are 4.85 × 108Individual/mL,
Effective bacteria containing amount of nitrous acid coccus bacterium solution is 3.97 × 108Individual/mL, effective bacteria containing amount of azotobacter chroococcum bacterium solution for 7.17 ×
108Effective bacteria containing amount of the double spore bacterium bacterium solutions of individual/mL, high temperature is 2.01 × 109Individual/mL
(2) preparation of culture medium：
A, in parts by weight, purslane, common perilla leaf, castor leaf, lemon, sodium onitrophenol is taken to be extracted respectively
Obtain common perilla leaf extract, castor-oil plant leaf extract, lemon chromocor extract；
A, the concrete operations of purslane extract are：Taking purslane, then obtaining content reaches 50% linoleic acid by water extraction
The extract solution of composition；
B, the concrete operations of common perilla leaf extract are：Take common perilla leaf to be crushed after being dried, extracted and filled by circulating ultrasonic
It is 1 to put according to comminuted powder and solvent for extraction according to volume ratio:2 ratio mixing extraction, 50 degrees Celsius of Extracting temperature, supersonic frequency
Rate is 50KHz, is extracted 2 minutes, circulation extraction 5 times, and 5 liquid are merged after filtering, and liquid loads solvent recovery enrichment facility 50
Degree Celsius recycling design, then pressure be 10MPa, temperature is 30 DEG C, CO2Flow 15kg/h, the time be 15 minutes, refined filtration,
Common perilla leaf extract is obtained, contains linoleic acid 35.4%, leukotrienes 56.5% in common perilla leaf extract；
C, the concrete operations of castor-oil plant leaf extract are：Take castor leaf by broken, soxhlet extraction degreasing, water extract-alcohol precipitation, from
The heart, water-bath are volatilized, are freeze-dried, and produce castor-oil plant leaf extract；
D, the concrete operations of lemon chromocor extract are：Lemon is taken to add water and cellulase, volume after carrying out peeling remove seed
Than for 1:1:0.02, digested, then after carrying out enzyme deactivation, obtain enzymolysis liquid, the pH of enzymolysis liquid is adjusted to after 4, uses second
Acetoacetic ester extracts, and takes organic phase, and the 1wt.% of weight such as addition sodium hydrate aqueous solution, stirs, enter in organic phase
Row extraction, phase of fetching water, aqueous phase feeding milipore filter is filtered, then the penetrating fluid of milipore filter is concentrated with NF membrane, then
After being dried, lemon chromocor extract is obtained；
B, in parts by weight, weigh ganoderma lucidum leftover bits and pieces, garlic juice, mung bean shell, wheat bran, Malus spectabilis complete stool, polygonum flaccidum grass juice factor,
Soya bean bran, jacket, the wood chip that deoils, shrub althea flower, fluffy sub- Lay, composite microbial bacteria, sodium onitrophenol, sodium selenite, selenium
Cysteine obtains base-material after building heap and fermenting 11 days；
C, and then by step A common perilla leaf extract, castor-oil plant leaf extract, lemon chromocor extract and the step B's obtained
Base-material mixing is follow-up to continue heap 22 hours, and controls water content to can obtain culture medium clinker 44%；
(3) it is inoculated with：The culture medium clinker that step (2) obtains is fed in kind of pocket, then carries out sterilization treatment, then connect
And aseptic inoculation is carried out in desinfection chamber, Ganoderma Lucidum is inoculated into kind of pocket；
(4) training orientation：Kind pocket after inoculation is stacked to fungus shelf, indoor temperature control enters the bacterium that walks at 29 DEG C
20 days, light reached 680LX in regulation culture room afterwards, and control indoor temperature is at 29 DEG C, and relative humidity control is 84%, indoor guarantor
Hold dark；
(5) harvest：When ganoderma lucidum cap deploys, cap starts keratin, starts to eject spore, you can harvesting.
Testing inspection data explanation：
Control group：The culture medium used for：75% wood chip, 20% wheat bran, 3% corn flour, 1% land plaster and 1% lightweight
What calcium carbonate was mixed to get, except culture medium for cultivating processing is different, the cultural method that remaining is used is identical with the step of embodiment 5；
By the ganoderma lucidum after embodiments of the invention 1-5, control group cultivation harvest, each group randomly selects 30 plants of ganoderma lucidums and pulverized,
Survey soluble protein content, soluble sugar content, Vitamin C content, ammonia nitrogen content, Se content.
Wherein：The detection of crude protein content：Micro triumphant examination nitriding measure；Total reducing sugar is using the fixed sugared method of Fei Linshi pyrotitrations；
Soluble sugar content uses anthrone colorimetry；Amino acid is detected using acid-hydrolysis method；Se content reference national standard GB5009.93-
2010 measure, measurement result see the table below：
Quality determination (average ± standard deviation n=3) in the ganoderma lucidum fruitbody of table 1
The upper right mark difference letter of each numerical value represents the significance of difference (P in upper table<0.05)
Understand that the quality of the ganoderma lucidum fruitbody of embodiment 1-5 productions is more preferable than control group by upper table data analysis, embodiment
Egg crude protein, total reducing sugar, essential amino acid, nonessential amino acid, Se content content is apparently higher than control group, it was demonstrated that, with implementation
The Se content that the method production ganoderma lucidum of example can fully absorb Medium Culture reaches selenium-rich, while can also meet professional standard.And lead to
The fructification that food inspection office of official provides our company is crossed to detect, wherein, moisture 13.5%, total ash content
For 1.7%, extract 8.6%, 17.0%, total ash content must not exceed with the moisture of the fructification of national regulation
3.2%, extract is must not exceed no less than 3.0% to be consistent；The profile of fructification is in umbrella, cap kidney shape, semicircle or circle
Shape, diameter reach 10-18 centimetres, and thick 1-2 centimetres, shell is hard, yellowish-brown to bronzing, glossy, have ring-type rib and spoke
Penetrate shape wrinkle, thin edge and truncate, bacterial context white to light brown, spore is tiny, yellowish-brown, gas micro-perfume, bitter and puckery flavor.
Embodiment 1-5, control group are each organized to 80 culture matrixes of processing, each packed 380 grams of clinkers of culture medium, culture
15 centimetres of the diameter of base bag, it is high 30 ± 2 centimetres after 380 grams of pack, after cultivation harvest, record every bag of equal yield, concrete condition
It see the table below.
The per mu yield of table 2, conversion ratio analytical table
Remarks：The compost dry weight * 100% of conversion ratio=edible mushroom fresh weight/used.
The ganoderma lucidum yield height and high conversion rate cultivated as seen from the above table using the culture medium of the application.
Using the technical scheme of the application, generation pest and disease damage probability is small (the probability statistics that disease occurs see the table below), survives
Rate is high, and the key factor increased income, and the probability that pest and disease damage occurs is few, and a major reason increased income.
The disease rate situation table of table 3
| Time | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Control group | |
| 10 days | Disease rate % | 0.1 | 0 | 0.1 | 0 | 0 | 1.2 |
| 20 days | Disease rate % | 0.3 | 0.2 | 0.3 | 0.3 | 0.2 | 1.6 |
| 30 days | Disease rate % | 0.3 | 0.3 | 0.3 | 0.4 | 0.5 | 1.8 |
| 40 days | Disease rate % | 0.3 | 0.5 | 0.6 | 0.4 | 0.5 | 1.9 |
Remarks：The surface area of disease rate %=disease culture mediums area/80 culture medium；Periphery product=lateral area
+ floor space × 2=3.14 × diameter × height+3.14 × radius2×2
The probability that disease occurs using the culture medium of the application as seen from the above table is far smaller than control group.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously
Therefore limitation of the scope of the invention can not be interpreted as.It should be pointed out that for the person of ordinary skill of the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention
Enclose.Therefore, protection scope of the present invention should be determined by the appended claims.
Claims (6)
- A kind of 1. implantation methods for improving ganoderma lucidum quality, it is characterised in that：Including step in detail below：(1) selection of base material is cultivated：Culture medium weighs 10-25 parts by weight soya beans bran in parts by weight, 10-20 parts by weight are gone Oily wood chip, 10-20 weight Ganoderma Lucidums leftover bits and pieces, 8-20 parts by weight wheat bran, 10-20 parts by weight mung bean shell, 5-15 parts by weight Ma Ling Potato skin, 5-15 parts by weight Malus spectabilis complete stool, 4-10 parts by weight castor leaf, 5-10 parts by weight shrub althea flower, the fluffy sub- Lay of 4-10 parts by weight, 5- 10 parts by weight purslanes, 3-10 parts by weight common perilla leaf, 3-10 parts by weight sodium onitrophenol, 4-10 parts by weight selenocystein, 5-10 parts by weight sodium selenite, 3-10 parts by weight of lemon, 3-10 parts by weight polygonum flaccidums grass juice factor, 5-10 parts by weight garlic juice, 5-10 weights Measure part composite microbial bacteria；(2) preparation of culture medium：A, in parts by weight, purslane, common perilla leaf, castor leaf, lemon, sodium onitrophenol is taken to be extracted to obtain respectively Common perilla leaf extract, castor-oil plant leaf extract, lemon chromocor extract；B, in parts by weight, ganoderma lucidum leftover bits and pieces, garlic juice, mung bean shell, wheat bran, Malus spectabilis complete stool, polygonum flaccidum grass juice factor, soya bean are weighed Bran, jacket, the wood chip that deoils, shrub althea flower, fluffy sub- Lay, composite microbial bacteria, sodium onitrophenol, sodium selenite, the Guang of selenium half Propylhomoserin obtains base-material after building heap and fermenting 7-12 days；C, and then by step A common perilla leaf extract, castor-oil plant leaf extract, the base-material of lemon chromocor extract and step B obtained Mix it is follow-up continue heap 12-24 hours, and control water content to can obtain culture medium clinker in 40-45%；(3) it is inoculated with：The culture medium clinker that step (2) obtains is fed in kind of pocket, then carries out sterilization treatment, followed by Aseptic inoculation is carried out in desinfection chamber, Ganoderma Lucidum is inoculated into kind of pocket；(4) training orientation：Kind pocket after inoculation is stacked to fungus shelf, indoor temperature control enters the bacterium that walks at 22-26 DEG C 20 days, light reached 600-700LX in regulation culture room afterwards, and at 25-30 DEG C, relative humidity controls in 80- control indoor temperature Between 85%, indoor holding is dark；(5) harvest：When ganoderma lucidum cap deploys, cap starts keratin, starts to eject spore, you can harvesting.
- A kind of 2. implantation methods for improving ganoderma lucidum quality according to claim 1, it is characterised in that：The culture medium be with What lower raw material was prepared：13-20 parts by weight soya beans bran, 12-18 parts by weight deoil wood chip, 12-17 weight Ganoderma Lucidums leftover bits and pieces, 10-17 parts by weight wheat bran, 13-17 parts by weight mung bean shell, 8-12 parts by weight jacket, 8-13 parts by weight Malus spectabilis complete stool, 5-8 weights Measure part castor leaf, 6-9 parts by weight shrub althea flower, the fluffy sub- Lay of 6-9 parts by weight, 6-9 parts by weight purslane, 5-9 parts by weight common perilla leaf, 5- 9 parts by weight sodium onitrophenols, 5-9 parts by weight selenocystein, 6-9 parts by weight sodium selenite, 5-9 parts by weight of lemon, 5-9 weights Measure part polygonum flaccidum grass juice factor, 6-9 parts by weight garlic juice, 6-9 parts by weight composite microbial bacterias.
- A kind of 3. implantation methods for improving ganoderma lucidum quality according to claim 1, it is characterised in that：The culture medium be with What lower raw material was prepared：16 parts by weight soya bean brans, 14 parts by weight are deoiled wood chip, 15 weight Ganoderma Lucidum leftover bits and pieces, 14 parts by weight Wheat bran, 15 parts by weight mung bean shells, 10 parts by weight jackets, 10 parts by weight Malus spectabilis complete stools, 7 parts by weight castor leaves, 7 parts by weight wood Rose of Sharon flower, the fluffy sub- Lay of 8 parts by weight, 8 parts by weight purslanes, 7 parts by weight common perilla leafs, 7 parts by weight sodium onitrophenols, 6 parts by weight selenium Cysteine, 7 parts by weight sodium selenites, 6 parts by weight of lemon, 7 parts by weight polygonum flaccidum grass juice factors, 8 parts by weight garlic juices, 7 parts by weight are answered Close microbial bacteria.
- A kind of 4. implantation methods for improving ganoderma lucidum quality according to claim 1, it is characterised in that：The complex microorganism The raw material that bacterium bag includes following parts by weight is prepared into：Se-enriched yeast bacterium solution 5-10 parts, the double spore bacterium bacterium solution 5-10 parts of high temperature, production alkali bar Bacterium bacterium solution 5-10 parts, nitrous acid coccus bacterium solution 5-12 parts, azotobacter chroococcum bacterium solution 5-10 parts, the double spore bacterium bacterium solution 4-12 parts of high temperature.
- A kind of 5. implantation methods for improving ganoderma lucidum quality according to claim 1, it is characterised in that：The complex microorganism The raw material that bacterium bag includes following parts by weight is prepared into：Double 8 parts of the spore bacterium bacterium solutions of 7 parts of Se-enriched yeast bacterium solution, high temperature, Bacillus alcaligenes bacterium solution 6 parts, 8 parts of nitrous acid coccus bacterium solution, 7 parts of azotobacter chroococcum bacterium solution, double 9 parts of the spore bacterium bacterium solutions of high temperature.
- A kind of 6. implantation methods of raising ganoderma lucidum quality according to claim 4 or 5, it is characterised in that：It is described compound micro- Effective bacteria containing amount of Se-enriched yeast bacterium solution in biological bacteria is 3.12 × 108-8.56×108The double spore bacterium bacterium solutions of individual/mL, high temperature Effective bacteria containing amount is 0.15 × 109-2.55×109Individual/mL, effective bacteria containing amount of Bacillus alcaligenes bacterium solution are 1.25 × 108-5.65 ×108Individual/mL, effective bacteria containing amount of nitrous acid coccus bacterium solution are 1.24 × 108-4.59×108Individual/mL, azotobacter chroococcum bacterium solution Effective bacteria containing amount be 5.26 × 108-8.54×108Effective bacteria containing amount of the double spore bacterium bacterium solutions of individual/mL, high temperature is 1.04 × 109- 2.12×109Individual/mL.
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| CN108934752A (en) * | 2018-06-28 | 2018-12-07 | 靖西市秀美边城农业科技有限公司 | A kind of method of substituting stuff cultivation giant glossy ganoderma |
| CN111296169A (en) * | 2020-03-12 | 2020-06-19 | 古田县鹤塘明艳茶叶专业合作社 | Green circulating annual production method for interplanting soybeans and lucid ganoderma |
| CN113079941A (en) * | 2021-04-08 | 2021-07-09 | 徐州工程学院 | Cultivation method and extraction method for increasing flavonoid content in phellinus igniarius sporocarp |
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