CN113079941A - Cultivation method and extraction method for increasing flavonoid content in phellinus igniarius sporocarp - Google Patents
Cultivation method and extraction method for increasing flavonoid content in phellinus igniarius sporocarp Download PDFInfo
- Publication number
- CN113079941A CN113079941A CN202110377625.XA CN202110377625A CN113079941A CN 113079941 A CN113079941 A CN 113079941A CN 202110377625 A CN202110377625 A CN 202110377625A CN 113079941 A CN113079941 A CN 113079941A
- Authority
- CN
- China
- Prior art keywords
- cultivation
- parts
- phellinus
- fruiting body
- phellinus igniarius
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000123113 Phellinus igniarius Species 0.000 title claims abstract description 50
- 229930003935 flavonoid Natural products 0.000 title claims abstract description 30
- 150000002215 flavonoids Chemical class 0.000 title claims abstract description 30
- 235000017173 flavonoids Nutrition 0.000 title claims abstract description 30
- 238000012364 cultivation method Methods 0.000 title claims abstract description 18
- 238000000605 extraction Methods 0.000 title claims abstract description 7
- 239000000463 material Substances 0.000 claims abstract description 42
- 235000008708 Morus alba Nutrition 0.000 claims abstract description 11
- 244000017020 Ipomoea batatas Species 0.000 claims abstract description 10
- 235000002678 Ipomoea batatas Nutrition 0.000 claims abstract description 10
- 229920002752 Konjac Polymers 0.000 claims abstract description 10
- 240000000249 Morus alba Species 0.000 claims abstract description 10
- 241000241413 Propolis Species 0.000 claims abstract description 9
- 229940069949 propolis Drugs 0.000 claims abstract description 9
- 244000247812 Amorphophallus rivieri Species 0.000 claims abstract description 7
- 235000001206 Amorphophallus rivieri Nutrition 0.000 claims abstract description 7
- 235000013312 flour Nutrition 0.000 claims abstract description 7
- 235000010485 konjac Nutrition 0.000 claims abstract description 7
- 239000000252 konjac Substances 0.000 claims abstract description 7
- 239000000758 substrate Substances 0.000 claims abstract description 3
- 241000001727 Tropicoporus linteus Species 0.000 claims description 26
- 238000000034 method Methods 0.000 claims description 20
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims description 14
- 239000001963 growth medium Substances 0.000 claims description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 11
- 238000005286 illumination Methods 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 10
- 239000007836 KH2PO4 Substances 0.000 claims description 7
- 239000000292 calcium oxide Substances 0.000 claims description 7
- 235000012255 calcium oxide Nutrition 0.000 claims description 7
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 238000003306 harvesting Methods 0.000 claims description 5
- 238000011081 inoculation Methods 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 4
- 239000002609 medium Substances 0.000 claims description 3
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 2
- 241000522190 Desmodium Species 0.000 claims description 2
- 241000219991 Lythraceae Species 0.000 claims description 2
- 235000014443 Pyrus communis Nutrition 0.000 claims description 2
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 8
- 241001312217 Zamioculcas zamiifolia Species 0.000 abstract description 6
- 241000233866 Fungi Species 0.000 abstract description 4
- 239000002994 raw material Substances 0.000 abstract description 3
- 230000003071 parasitic effect Effects 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 14
- 238000009423 ventilation Methods 0.000 description 6
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 5
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 5
- 235000013399 edible fruits Nutrition 0.000 description 5
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 5
- 229930003944 flavone Natural products 0.000 description 5
- 150000002212 flavone derivatives Chemical class 0.000 description 5
- 235000011949 flavones Nutrition 0.000 description 5
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 5
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 5
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 5
- 235000005493 rutin Nutrition 0.000 description 5
- 229960004555 rutoside Drugs 0.000 description 5
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 5
- 239000003814 drug Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 241000123107 Phellinus Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000013543 active substance Substances 0.000 description 3
- 239000011344 liquid material Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000222382 Agaricomycotina Species 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 241000222383 Polyporales Species 0.000 description 2
- 241000219000 Populus Species 0.000 description 2
- 241001556385 Sanghuangporus baumii Species 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- BNGXYYYYKUGPPF-UHFFFAOYSA-M (3-methylphenyl)methyl-triphenylphosphanium;chloride Chemical compound [Cl-].CC1=CC=CC(C[P+](C=2C=CC=CC=2)(C=2C=CC=CC=2)C=2C=CC=CC=2)=C1 BNGXYYYYKUGPPF-UHFFFAOYSA-M 0.000 description 1
- 201000000736 Amenorrhea Diseases 0.000 description 1
- 206010001928 Amenorrhoea Diseases 0.000 description 1
- 244000144730 Amygdalus persica Species 0.000 description 1
- 244000274847 Betula papyrifera Species 0.000 description 1
- 235000009113 Betula papyrifera Nutrition 0.000 description 1
- 235000009109 Betula pendula Nutrition 0.000 description 1
- 235000010928 Betula populifolia Nutrition 0.000 description 1
- 235000002992 Betula pubescens Nutrition 0.000 description 1
- 206010016654 Fibrosis Diseases 0.000 description 1
- 206010027514 Metrorrhagia Diseases 0.000 description 1
- 241000218213 Morus <angiosperm> Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 244000305267 Quercus macrolepis Species 0.000 description 1
- 241000124033 Salix Species 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 241000190021 Zelkova Species 0.000 description 1
- 231100000540 amenorrhea Toxicity 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004761 fibrosis Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Substances [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention belongs to the technical field of artificial cultivation of edible fungi, and particularly relates to a cultivation method and an extraction method for improving the flavonoid content in phellinus igniarius sporocarp. The cultivation method for improving the flavonoid content in the phellinus igniarius sporocarp is characterized in that the phellinus igniarius sporocarp with high content of flavonoids is optimized and developed on the basis of artificial bag material cultivation, mulberry twig sawdust is used as a cultivation substrate, and peel residue, sweet potato vine, konjac flour, zamioculcas zamiifolia and propolis are added as nutritional raw materials, so that the nutritional requirement of phellinus igniarius cultivation can be met, the flavonoid content in the cultivated phellinus igniarius sporocarp is effectively improved in an induced mode, and the nutritional parasitic application value of phellinus igniarius is effectively improved.
Description
Technical Field
The invention belongs to the technical field of artificial cultivation of edible fungi, and particularly relates to a cultivation method and an extraction method for improving the flavonoid content in phellinus igniarius sporocarp.
Background
Phellinus igniarius (Phellinus igniarius) belongs to Basidiomycotina (Basidiomycotina), Hymenomycetes (Hymenomycetes), Aphyllophorales (Aphyllophorales), Hymenochaeyaceae (Hymenochaeyaceae), Phellinus (Phellinus), and is the trade name of Phellinus igniarius (P.igniarius), Phellinus baumii (P.baumii) and Phellinus linteus (P.Linteus), and is generally grown on the stems of withered trees and stumps of broad-leaved trees such as poplar, mulberry, willow, white birch, zelkova, peach, and the like. Phellinus linteus is a precious medicinal fungus developed in recent years for many years and is named as forest gold in the name of America. According to the records of Chinese medicine dictionary, Phellinus can be used for treating gynecological diseases such as metrorrhagia, bloody stranguria, leukorrhagia, amenorrhea, etc.; modern medical research also shows that phellinus igniarius has remarkable effects of resisting tumor, bacteria and fibrosis, resisting oxidation, improving human immunity and the like, is the rare medicinal fungus with the first biological anti-tumor effect internationally acknowledged at present, and is widely applied to the industries of medicines, foods, daily chemicals, health care products and the like.
At present, due to the restriction of the particularity and complexity of physiological states and external environment, the formation of the fruit body of phellinus igniarius in nature is difficult, so that the natural phellinus igniarius is rare in quantity, the resource available for medicine in nature is limited, and the demand of domestic and foreign markets for phellinus igniarius is increased, so that medicine farmers in domestic places take away to collect the phellinus igniarius, the fruit body cannot be formed in large quantity, the phellinus igniarius cannot become a stable industrial product source, and the development of the phellinus igniarius industry is very unfavorable, so that the defect of wild resources is made up through artificial cultivation, and the method is very important. However, artificial cultivation of Phellinus linteus is extremely difficult, and has problems of harsh culture conditions and long growth cycle.
At present, two methods are mainly used for artificially cultivating phellinus igniarius, one of the methods is mainly the cut-log cultivation of mulberry trees, poplar trees and oak trees, but the cut-log cultivation needs a large amount of wood resources, the resource waste is serious, and the large-scale cultivation is difficult; the other is bag cultivation, but has the problems of insufficient nutrient supply of the culture medium, small fruit body development, easy contamination of mixed bacteria and the like, and particularly, the content of active substances is not ideal.
Disclosure of Invention
Therefore, the technical problem to be solved by the invention is to provide a cultivation method for increasing the flavonoid content in phellinus igniarius sporocarp, so as to solve the problem that the content of flavonoid active substances in phellinus igniarius sporocarp cultivated by artificial bagged materials in the prior art is low;
the second technical problem to be solved by the invention is to provide a method for extracting flavonoid active substances from phellinus igniarius sporocarp.
In order to solve the technical problems, the cultivation method for improving the flavonoid content in the phellinus igniarius sporocarp comprises the following steps:
(1) preparing a cultivation bag material: taking 20-30 parts of mulberry twig sawdust, 10-20 parts of peel residues, 3-8 parts of sweet potato stems, 5-12 parts of konjac flour, 3-8 parts of loosestrife, 1-3 parts of propolis, 1-5 parts of quick lime and KH2PO40.3-0.8 weight part, and uniformly mixing to obtain the required culture medium; adding water into the culture medium, mixing uniformly, and bagging for later use;
(2) mycelium culture: inoculating a phellinus igniarius mother seed into the cultivation bag material, and carrying out constant-temperature cultivation on the inoculated cultivation bag material until the cultivation bag material is full of mycelia;
(3) and (3) fruiting body culture: cutting the bag material full of mycelia to produce mushroom, continuously performing constant temperature culture until the fruiting body is mature, and harvesting.
Specifically, in the step (1), the cultivation bag material comprises the following components: 25 parts of mulberry twig sawdust, 15 parts of peel residues, 5 parts of sweet potato vine, 8 parts of konjaku flour, 5 parts of desmodium, 2 parts of propolis, 3 parts of quick lime and KH2PO40.5 part by weight.
Specifically, in the step (1), the peel dregs comprise apple peel dregs, orange peel dregs and/or pear peel dregs.
Specifically, in the step (1), the mass ratio of the culture medium to water is 40-50%: 50 to 60 percent.
Specifically, in the step (2), the inoculation amount of the phellinus igniarius mother seeds is 5-20 wt% of the culture medium.
Specifically, in the step (2), the process conditions of the mycelium culturing step include: the culture temperature is controlled at 20-28 deg.C, air humidity is 50-70%, and illumination intensity is 10-20 lux.
Specifically, in the step (3), the process conditions of the fruiting body cultivation step include: the culture temperature is controlled at 20-28 deg.C, air humidity is 50-70%, and illumination intensity is 20-80 lux.
The invention also discloses a phellinus igniarius sporocarp cultivated by the method.
The invention also discloses a method for extracting flavonoid substances from the phellinus igniarius sporocarp, which comprises the step of carrying out alcohol extraction by taking the phellinus igniarius sporocarp as a raw material.
Specifically, the alcohol extraction step is to extract by using ethanol water solution with the concentration of 50-70 v/v%.
The cultivation method for improving the flavonoid content in the phellinus igniarius sporocarp is characterized in that the phellinus igniarius sporocarp with high content of flavonoids is optimized and developed on the basis of artificial bag material cultivation, mulberry twig sawdust is used as a cultivation substrate, and pericarp residues, sweet potato vines, konjac flour, zamioculcas zamiifolia and propolis are added as nutritional raw materials, so that the nutritional requirement of phellinus igniarius cultivation can be met, the flavonoid content in the cultivated phellinus igniarius sporocarp is effectively improved through induction, and the nutrition and application value of phellinus igniarius are effectively improved.
Detailed Description
In the following examples of the present invention, the phellinus linteus mother strain is obtained by separation and purification by a conventional method, activated by a conventional PDA plate medium, and inoculated into a conventional PD mother strain medium for culture, thereby obtaining the phellinus linteus mother strain.
Example 1
The cultivation method for improving the flavonoid content in the phellinus igniarius sporocarp comprises the following steps:
(1) preparing a cultivation bag material: taking 20 parts by weight of mulberry twig sawdust, 20 parts by weight of apple peel residues, 3 parts by weight of crushed sweet potato stems, 12 parts by weight of konjac block powder, 3 parts by weight of zamioculcas zamiifolia, 3 parts by weight of propolis, 1 part by weight of quick lime and KH2PO40.8 part by weight, and uniformly mixing to obtain the required culture medium; taking the culture medium according to the ratio of 50%: adding 50% of water by mass, mixing uniformly, and bagging for later use;
(2) mycelium culture: inoculating a phellinus igniarius mother seed liquid material into the cultivation bag material obtained in the step (1) according to the inoculation amount of 10%, placing the inoculated cultivation bag material at the temperature of 25 ℃ for constant-temperature cultivation, controlling the air humidity to be 60% and the illumination condition to be 15lux, keeping the cultivation time in a spaced ventilation state, and observing the cultivation condition of the bag material at any time until the cultivation bag material is full of mycelia;
(3) and (3) fruiting body culture: cutting a cut on the bag material full of mycelia for fruiting, continuously controlling the temperature to be 25 ℃, the air humidity to be 60% and the illumination intensity to be 50lux for constant-temperature culture, keeping the interval ventilation state between cultures, observing the fruiting condition of the bag material at any time, and harvesting in time.
Example 2
The cultivation method for improving the flavonoid content in the phellinus igniarius sporocarp comprises the following steps:
(1) preparing a cultivation bag material: taking 30 parts by weight of mulberry twig sawdust, 10 parts by weight of orange peel residues, 8 parts by weight of crushed sweet potato stems, 5 parts by weight of konjac block powder, 8 parts by weight of zamioculcas zamiifolia, 1 part by weight of propolis, 5 parts by weight of quick lime and KH2PO40.3 part by weight, and uniformly mixing to obtain the required culture medium; getThe culture medium comprises the following components in percentage by weight: adding 50% of water by mass, mixing uniformly, and bagging for later use;
(2) mycelium culture: inoculating a phellinus igniarius mother seed liquid material into the cultivation bag material obtained in the step (1) according to the inoculation amount of 10%, placing the inoculated cultivation bag material at the temperature of 25 ℃ for constant-temperature cultivation, controlling the air humidity to be 60% and the illumination condition to be 15lux, keeping the cultivation time in a spaced ventilation state, and observing the cultivation condition of the bag material at any time until the cultivation bag material is full of mycelia;
(3) and (3) fruiting body culture: cutting a cut on the bag material full of mycelia for fruiting, continuously controlling the temperature to be 25 ℃, the air humidity to be 60% and the illumination intensity to be 50lux for constant-temperature culture, keeping the interval ventilation state between cultures, observing the fruiting condition of the bag material at any time, and harvesting in time.
Example 3
The cultivation method for improving the flavonoid content in the phellinus igniarius sporocarp comprises the following steps:
(1) preparing a cultivation bag material: 25 parts of mulberry twig sawdust, 10 parts of apple peel residues, 5 parts of orange peel residues, 5 parts of crushed sweet potato vines, 8 parts of konjac block powder, 5 parts of zamioculcas zamiifolia, 2 parts of propolis, 3 parts of quick lime and KH2PO40.5 part by weight, and uniformly mixing to obtain the required culture medium; taking the culture medium according to the ratio of 50%: adding 50% of water by mass, mixing uniformly, and bagging for later use;
(2) mycelium culture: inoculating a phellinus igniarius mother seed liquid material into the cultivation bag material obtained in the step (1) according to the inoculation amount of 10%, placing the inoculated cultivation bag material at the temperature of 25 ℃ for constant-temperature cultivation, controlling the air humidity to be 60% and the illumination condition to be 15lux, keeping the cultivation time in a spaced ventilation state, and observing the cultivation condition of the bag material at any time until the cultivation bag material is full of mycelia;
(3) and (3) fruiting body culture: cutting a cut on the bag material full of mycelia for fruiting, continuously controlling the temperature to be 25 ℃, the air humidity to be 60% and the illumination intensity to be 50lux for constant-temperature culture, keeping the interval ventilation state between cultures, observing the fruiting condition of the bag material at any time, and harvesting in time.
Comparative example 1
The method for cultivating phellinus linteus in the comparative example is the same as example 3, and only the difference is that no zamioculcas zamiifolia is added into the cultivation bag material.
Comparative example 2
The method for cultivating phellinus linteus in the comparative example is the same as example 3, and is different from the method in the embodiment in that the same amount of sucrose is used to replace the peel residue in the cultivation bag material.
Comparative example 3
The method for cultivating phellinus linteus in the comparative example is the same as example 3, and is different from the method in the embodiment that the sweet potato vine is not added into the cultivation bag material.
Comparative example 4
The method for cultivating phellinus linteus in this comparative example is the same as example 3, except that the konjaku flour is not added to the cultivation bag material.
Examples of the experiments
1. Phellinus igniarius sporophore weight and mass
The weight and mass of the fruiting bodies of Phellinus linteus harvested in example 3 and comparative examples 1-4 were recorded, and the results are shown in Table 1 below.
TABLE 1 Phellinus linteus fruiting body culture
| Numbering | Weight of fruiting body/g | Quality and shape of fruiting body |
| Example 3 | 41g | Preferably, it is close to wild |
| Comparative example 1 | 40g | Preferably, it is close to wild |
| Comparative example 2 | 36g | Preferably, it is close to wild |
| Comparative example 3 | 40g | Preferably, it is close to wild |
| Comparative example 4 | 38g | Preferably, it is close to wild |
2. Flavone content in Phellinus linteus fruiting body
The phellinus linteus fruit bodies harvested in the above example 3 and comparative examples 1 to 4 were respectively washed, dried and pulverized, 3 times of ethanol aqueous solution (70 v/v%) was added to the pulverized phellinus linteus material to extract for 8 hours for 2 times in total, the extract solutions were collected and combined, the content of flavonoids in the extract solution was measured after concentration, and the measurement of the flavone content was carried out using commercially available wild phellinus linteus fruit bodies as a control, and the measurement results are recorded in table 2 below.
In the experimental example, the content of the total flavone is determined by a rutin standard sample according to a general method in the prior art, and the method specifically comprises the following steps: weighing 0.010g of rutin, dissolving with 30% ethanol, and diluting to a volume of 100ml in a volumetric flask to prepare a 0.1mg/ml rutin standard solution; respectively sucking 0.0, 2.0, 4.0, 6.0, 8.0, and 10.0ml of the rutin standard solution, placing in 6 volumetric flasks of 50ml, numbering 0-5 in sequence, supplementing 10ml with 30% ethanol, adding 0.8ml of 5% NaNO2Shaking, standing for 6min, adding 0.8ml 10% aluminum nitrate, shaking, standing for 6min, adding 10ml 1mol/l sodium hydroxide, shaking for 15min, developingThe absorbance was measured spectrophotometrically at 510 nm. And measuring the absorbance of the flavone in the extracting solution under the condition, and reading the content of the flavone on a standard curve to obtain the content of rutin.
TABLE 2 flavonoid content in Phellinus Linteus
From the above results, it can be seen that the quality of the phellinus igniarius sporocarp is improved, the content of flavonoid substances in the phellinus igniarius sporocarp is effectively increased by adjusting the nutritional ingredients in the phellinus igniarius artificial cultivation bag material, and the nutritional and medicinal values of the phellinus igniarius sporocarp are improved.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.
Claims (10)
1. A cultivation method for increasing the content of flavonoids in phellinus igniarius sporocarp is characterized by comprising the following steps:
(1) preparing a cultivation bag material: taking 20-30 parts of mulberry twig sawdust, 10-20 parts of peel residues, 3-8 parts of sweet potato stems, 5-12 parts of konjac flour, 3-8 parts of loosestrife, 1-3 parts of propolis, 1-5 parts of quick lime and KH2PO40.3-0.8 weight part, and uniformly mixing to obtain the required culture medium; adding water into the culture medium, mixing uniformly, and bagging for later use;
(2) mycelium culture: inoculating a phellinus igniarius mother seed into the cultivation bag material, and carrying out constant-temperature cultivation on the inoculated cultivation bag material until the cultivation bag material is full of mycelia;
(3) and (3) fruiting body culture: cutting the bag material full of mycelia to produce mushroom, continuously performing constant temperature culture until the fruiting body is mature, and harvesting.
2. The cultivation method for increasing the flavonoid content in phellinus linteus fruiting body according to claim 1, wherein in the step (1), the cultivation bag material comprises the following components: 25 parts of mulberry twig sawdust, 15 parts of peel residues, 5 parts of sweet potato vine, 8 parts of konjaku flour, 5 parts of desmodium, 2 parts of propolis, 3 parts of quick lime and KH2PO40.5 part by weight.
3. The cultivation method for increasing the flavonoid content in phellinus linteus fruiting body according to claim 1 or 2, wherein in the step (1), the peel dregs comprise apple peel dregs, orange peel dregs and/or pear peel dregs.
4. The cultivation method for increasing the flavonoid content in phellinus linteus fruiting body according to any one of claims 1 to 3, wherein in the step (1), the mass ratio of the cultivation substrate to water is 40-50%: 50 to 60 percent.
5. The cultivation method for increasing the flavonoid content in phellinus igniarius sporocarp according to any one of claims 1 to 4, wherein in the step (2), the inoculation amount of the mother phellinus igniarius is 5 to 20 wt% of the cultivation medium.
6. The cultivation method for increasing the flavonoid content in phellinus linteus fruiting body according to any one of claims 1 to 5, wherein in the step (2), the process conditions of the mycelium cultivation step include: the culture temperature is controlled at 20-28 deg.C, air humidity is 50-70%, and illumination intensity is 10-20 lux.
7. The cultivation method for increasing the flavonoid content in phellinus linteus fruiting body according to any one of claims 1 to 6, wherein in the step (3), the process conditions of the fruiting body cultivation step include: the culture temperature is controlled at 20-28 deg.C, air humidity is 50-70%, and illumination intensity is 20-80 lux.
8. Phellinus linteus fruiting body cultivated by the method according to any one of claims 1 to 7.
9. A method for extracting flavonoids from Phellinus linteus fruiting body, which comprises extracting with ethanol the Phellinus linteus fruiting body of claim 8.
10. The method for extracting flavonoids from Phellinus linteus fruiting body as claimed in claim 9, wherein the alcohol extraction step is carried out by extracting with 50-70 v/v% ethanol water solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110377625.XA CN113079941A (en) | 2021-04-08 | 2021-04-08 | Cultivation method and extraction method for increasing flavonoid content in phellinus igniarius sporocarp |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110377625.XA CN113079941A (en) | 2021-04-08 | 2021-04-08 | Cultivation method and extraction method for increasing flavonoid content in phellinus igniarius sporocarp |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113079941A true CN113079941A (en) | 2021-07-09 |
Family
ID=76675493
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110377625.XA Pending CN113079941A (en) | 2021-04-08 | 2021-04-08 | Cultivation method and extraction method for increasing flavonoid content in phellinus igniarius sporocarp |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113079941A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116391568A (en) * | 2023-05-19 | 2023-07-07 | 吉林农业科技学院 | Phellinus linteus strain culture medium and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107455142A (en) * | 2017-08-29 | 2017-12-12 | 田林县群英农业有限公司 | A kind of implantation methods for improving ganoderma lucidum quality |
| CN112189505A (en) * | 2020-08-28 | 2021-01-08 | 徐州工程学院 | Artificially-cultured phellinus igniarius sporophore extract for treating gout and preparation method thereof |
-
2021
- 2021-04-08 CN CN202110377625.XA patent/CN113079941A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107455142A (en) * | 2017-08-29 | 2017-12-12 | 田林县群英农业有限公司 | A kind of implantation methods for improving ganoderma lucidum quality |
| CN112189505A (en) * | 2020-08-28 | 2021-01-08 | 徐州工程学院 | Artificially-cultured phellinus igniarius sporophore extract for treating gout and preparation method thereof |
Non-Patent Citations (5)
| Title |
|---|
| 刘向前等: "中国产和韩国产芸香科植物的成分比较研究", 《中草药》 * |
| 戚跃科等: "野生和栽培马齿苋黄酮含量的比较研究", 《丽水学院学报》 * |
| 李文芳等: "甘薯叶和茎中黄酮类化合物含量的初步测定", 《中国农学通报》 * |
| 陈江萍: "马齿苋的开发利用", 《陕西林业科技》 * |
| 黄磊等: "食用菌固体培养基的开发与应用综述", 《湖北农业科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116391568A (en) * | 2023-05-19 | 2023-07-07 | 吉林农业科技学院 | Phellinus linteus strain culture medium and preparation method thereof |
| CN116391568B (en) * | 2023-05-19 | 2023-08-29 | 吉林农业科技学院 | Phellinus linteus strain culture medium and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103330258B (en) | Cordyceps militaris health-care beverage prepared by liquid submerged fermentation and preparation method thereof | |
| Daba et al. | Production of mushroom (Pleurotus ostreatus) in Egypt as a source of nutritional and medicinal food | |
| CN104920065B (en) | A kind of wild Dictyophora rubrovalvata parent species preparation method of Mount Fanjing | |
| CN111534441A (en) | Rapid cultivation method of ganoderma lucidum, ganoderma lucidum oral liquid and preparation method thereof | |
| CN107299063A (en) | Termitomyces albuminosus with black skin liquid spawn preparation method | |
| CN105557294A (en) | Artificial culture method for Antrodia camphorata by utilizing basswood | |
| CN104206169A (en) | Method for preparing nutrient cereal by cordyceps militaris culture medium | |
| CN103664326A (en) | Pleurotus nebrodensis cultivation medium imitating wild cultivation and preparation method thereof | |
| CN103250556A (en) | Method for cultivating rhodiola rosea bag material healthcare pleurotus nebrodensis | |
| CN108175040B (en) | Preparation method of millet oat antrodia camphorata mycoplasm health food | |
| TWI422680B (en) | Culture medium for culturing fruiting bodies of antrodia cinnamomea and method for culturing the same | |
| CN113079941A (en) | Cultivation method and extraction method for increasing flavonoid content in phellinus igniarius sporocarp | |
| CN115340952A (en) | Method for improving quality of fermentation mycelium of phyllobacterium ribrum by using honeysuckle stem extract | |
| CN104823716A (en) | Culture and preparing method of fungus symbiotic hypha powder | |
| CN107573122B (en) | The liquid fermentation culturing method and culture medium of Antrodia camphorata | |
| CN112715351B (en) | Rapid breeding method of phellinus igniarius | |
| TWI688333B (en) | Chinese medicine medium for improving nutrient composition of flammulina velutipes and preparation method thereof | |
| CN117946871A (en) | Method for separating and cultivating strain from tricholoma matsutake fruiting body | |
| CN109328865B (en) | Preparation method of cordyceps militaris and cordyceps militaris prepared by same | |
| CN115039633A (en) | Artificial culture method for sporocarp of Isaria japonica | |
| CN113079942A (en) | Cultivation method and extraction method for increasing polyphenol content in phellinus igniarius sporocarp | |
| CN112514736A (en) | Method for cultivating lentinus edodes by using stevia rebaudiana leaf residues | |
| CN112189506A (en) | Method for efficiently cultivating phellinus igniarius sporocarp based on artificial bagged materials | |
| CN112314918A (en) | Cordyceps sinensis and chestnut paste | |
| CN113079943A (en) | Cultivation method and extraction method for increasing content of terpenoid in phellinus igniarius sporocarp |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210709 |