CN111534441A - Rapid cultivation method of ganoderma lucidum, ganoderma lucidum oral liquid and preparation method thereof - Google Patents
Rapid cultivation method of ganoderma lucidum, ganoderma lucidum oral liquid and preparation method thereof Download PDFInfo
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
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- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
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- A61K9/00—Medicinal preparations characterised by special physical form
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
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- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
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- A61P25/00—Drugs for disorders of the nervous system
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/20—Hypnotics; Sedatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
Abstract
The invention discloses a rapid cultivation method of ganoderma lucidum, ganoderma lucidum oral liquid and a preparation method thereof. The rapid cultivation method comprises the steps of obtaining primary ganoderma lucidum strains; obtaining cultured ganoderma lucidum strains through multi-stage culture, and dividing the culture into the first-stage ganoderma lucidum strain culture and the second-stage ganoderma lucidum strain culture; artificially culturing with second-stage Ganoderma strain to obtain cultured Ganoderma, and obtaining Ganoderma culture solution and Ganoderma culture residue. The ganoderma lucidum oral liquid is prepared from the following raw materials in parts by weight: the ganoderma lucidum oral liquid is prepared by adding water into ganoderma lucidum culture residues, heating to boil, filtering, adding supernatant into ganoderma lucidum culture liquid to obtain ganoderma lucidum mixed liquid, soaking the ganoderma lucidum mixed liquid and the second-stage ganoderma lucidum mycelia in water, and mixing the filtered ganoderma lucidum liquid to obtain ganoderma lucidum oral liquid. The method for preparing the cultured ganoderma lucidum from the primary ganoderma lucidum is easy to culture, high in speed, high in yield and high in nutritional value.
Description
Technical Field
The invention belongs to the technical field of medicines, and particularly relates to a rapid cultivation method of ganoderma lucidum, ganoderma lucidum oral liquid and a preparation method thereof.
Background
Meat ganoderma, commonly known as Taisui. The ganoderma lucidum is a very rare 'super-huge rare mythic fungus complex' in the nature, is a mythic fungus between protists and fungi according to the analysis of 'life origin and evolution pedigree diagram', has strong vitality, not only has the characteristics of the protists, but also has the characteristics of the fungi, and is the origin of animal and plant life. The slime mold has strong activity, is mainly propagated by spores and hyphae, has strong regeneration capacity, can be cut at will and can be regenerated. Once the slime mold loses the environment on which the slime mold lives, the slime mold enters a dormant period, even if the slime mold does not contain water, the slime mold cannot die easily, and once the slime mold is in the environment suitable for living, the slime mold wakes up and continues to grow. However, the original ganoderma lucidum has the defects of small yield, slow growth speed and poor nutrition.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a rapid cultivation method of ganoderma lucidum. The invention also aims to provide the ganoderma lucidum oral liquid. The invention also aims to provide a preparation method of the ganoderma lucidum oral liquid.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a rapid cultivation method of ganoderma lucidum comprises the following steps:
firstly, obtaining a raw ganoderma lucidum strain;
secondly, obtaining cultured ganoderma lucidum strains through multi-stage culture, and dividing the culture into the first-stage ganoderma lucidum strain culture and the second-stage ganoderma lucidum strain culture;
and thirdly, artificially culturing with the second-level ganoderma lucidum strain to obtain cultured ganoderma lucidum.
Further, the method for obtaining the primary ganoderma lucidum strain comprises the following specific steps:
step 1) selecting first-class native meat ganoderma, cleaning the first-class native meat ganoderma by using clear water, cutting off a part with the thickness of about 3 mm on the surface layer of the first-class native meat ganoderma by using a scalpel subjected to high-temperature disinfection, and cutting a sample of 2 cubic centimeters from the inside of the first-class native meat ganoderma;
step 2) putting the sample into medical alcohol with the concentration of 75%, and immersing the surface of the sample with the alcohol for 20 minutes to thoroughly sterilize the sample;
and 3) after soaking, naturally drying the taken sample in an aseptic box, cutting the sample into pieces by using an aseptic scalpel, putting the pieces into a clean test tube, and plugging the mouth of the test tube by using a rubber plug to obtain the primary ganoderma lucidum strain.
Further, the test tube has a size of 15 mm × 150 mm.
Further, the first-level ganoderma lucidum strain is cultured by primary ganoderma lucidum strain and a culture medium; weighing appropriate amount of glucose, royal jelly, peptone, beef extract, agar and NaCl, putting into a beaker, adding 350ml of water, stirring with a glass rod, and putting on an asbestos mesh for heating to dissolve; after the medicine is completely dissolved, supplementing water to 400 ml; measuring the pH value of the sample by using a precise pH test paper, and adjusting the pH value of the sample by using an lmol/L HCl solution and an lmol/L NaOH solution to make the pH value of the sample be 7; cleaning Lentinus Edodes and Ganoderma, boiling with water, filtering the boiled liquid, mixing with 100ml of the above culture medium, and sterilizing; when the temperature is reduced to 40 ℃, 15ml of the solution is added into a test tube containing the raw ganoderma lucidum, and the test tube is sealed and cultured for about 30 days, so that the first-grade ganoderma lucidum strain is obtained.
Furthermore, the formula of the culture medium for culturing the first-level ganoderma lucidum strain comprises, by weight, 5g of glucose, 0.5g of royal jelly, 50g of shiitake mushroom, 50g of ganoderma lucidum, lg of peptone, 0.3g of beef extract, 2g of agar and 0.5g of NaCl.
Further, the sterilization condition is 1.05kg/cm2And maintained at 121.3 deg.c for 20 min.
Further, the second-level ganoderma lucidum strain is cultured by the first-level ganoderma lucidum strain and a culture medium;
weighing maca, artificially-planted ginseng, cordyceps militaris, oyster, rhizoma polygonati, ginger, dried orange peel, cinnamon, Chinese yam, liquorice, wolfberry fruit, cassia seed, Chinese date, bee pollen, mulberry, fructus amomi, arillus longan, endothelium corneum gigeriae galli, fingered citron, spina date seed and hawthorn, pretreating, crushing and sieving to obtain raw material powder; adding 3-5 parts by weight of water into the raw material powder, then adding honey, brown sugar and 10 parts of ganoderma lucidum stock solution, sterilizing, and naturally cooling to obtain a culture medium; placing the culture medium into an aseptic container, adding the rest raw liquid of Ganoderma lucidum, mixing, adding Ganoderma lucidum, sealing, incubating at 28-33 deg.C, stirring for 3-5 days, stirring for 1-2 times per day, stirring for 5-10 days, stopping stirring, incubating for 3-5 months, collecting Ganoderma lucidum thallus to obtain Ganoderma lucidum thallus, and filtering the rest materials to obtain Ganoderma lucidum culture solution and Ganoderma lucidum culture residue.
Furthermore, the formula of the culture medium for culturing the second-level ganoderma lucidum strain comprises the following components in parts by weight:
8-12 parts of maca, 8-12 parts of artificially-planted ginseng, 0.2-0.5 part of cordyceps militaris, 4-6 parts of oyster, 4-6 parts of rhizoma polygonati, 2-4 parts of ginger, 4-6 parts of dried orange peel, 05-1.5 parts of cinnamon, 2-4 parts of Chinese yam, 0.5-1.5 parts of liquorice, 0.5-1.5 parts of wolfberry, 2-5 parts of semen cassiae, 2-5 parts of Chinese date, 0.1-0.5 part of bee pollen, 2-5 parts of mulberry, 1-2 parts of fructus amomi, 0.3-0.6 part of arillus longan, 1-3 parts of endothelium corneum gigeriae galli, 0.5-2 parts of fingered citron, 0.5-2 parts of spina date seed, 4-6 parts of hawthorn, 6-10 parts of honey and 6-10 parts of brown sugar.
Preferably, the formula of the culture medium for culturing the second-level ganoderma lucidum strain comprises the following components in parts by weight: 10 parts of maca, 10 parts of artificially-planted ginseng, 3 parts of cordyceps militaris, 5 parts of oyster, 5 parts of rhizoma polygonati, 3 parts of ginger, 5 parts of dried orange peel, 0.5 part of cinnamon, 3 parts of Chinese yam, 1 part of liquorice, 1 part of wolfberry fruit, 3 parts of semen cassiae, 3 parts of Chinese date, 0.3 part of bee pollen, 3 parts of mulberry, 1.5 parts of fructus amomi, 0.5 part of longan pulp, 2 parts of endothelium corneum gigeriae galli, 1 part of fingered citron, 1 part of spina date seed, 5 parts of hawthorn, 5 parts of honey and 5 parts of brown sugar.
The ganoderma lucidum oral liquid is prepared from the following raw materials in parts by weight: 1-2 parts of second-level ganoderma lucidum thallus, 1-2 parts of ganoderma lucidum culture liquid, 3-5 parts of ganoderma lucidum culture slag and 80-120 parts of water.
Further, the raw materials also comprise 0.01-0.03 part of edible salt.
A preparation method of the ganoderma lucidum oral liquid comprises the following steps:
adding mature second-level ganoderma lucidum mycelia into 10-30 parts of water, soaking, and filtering to obtain ganoderma lucidum liquid;
adding the rest part by weight of water into the ganoderma lucidum culture residues, heating to boil, preserving heat for 1-3h, filtering, adding the supernatant into ganoderma lucidum culture solution, and uniformly mixing to obtain ganoderma lucidum mixed solution;
and (3) mixing the ganoderma lucidum liquid obtained in the step (1) and the ganoderma lucidum mixed liquid obtained in the step (2) to obtain the ganoderma lucidum oral liquid.
Further, a proper amount of edible salt is added after the meat ganoderma lucidum liquid and the meat ganoderma lucidum mixed liquid are mixed.
Has the advantages that: the method for preparing the cultured ganoderma lucidum from the primary ganoderma lucidum has the advantages that the cultured ganoderma lucidum grows fast, the nutritive value is high, the yield is high, the cultured ganoderma lucidum is easy to culture, the production speed and the yield of the ganoderma lucidum are improved, and the nutritive value of the ganoderma lucidum is also improved; and finally realizing the rapid production of the ganoderma lucidum oral liquid through the secondary ganoderma lucidum cultivation.
Detailed Description
The invention is illustrated below with reference to specific examples. It will be understood by those skilled in the art that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention in any way.
Example 1
A rapid cultivation method of ganoderma lucidum comprises the following steps:
firstly, obtaining primary ganoderma lucidum strains:
(a) selecting first-class primary meat ganoderma lucidum, cleaning the first-class primary meat ganoderma lucidum by using clear water, cutting off a part with the thickness of about 3 mm on the surface layer of the first-class primary meat ganoderma lucidum by using a scalpel subjected to high-temperature disinfection, and cutting a sample of 2 cubic centimeters from the inside of the first-class primary meat ganoderma lucidum;
(b) putting the sample into medical alcohol with the concentration of 75%, immersing the surface of the sample with the alcohol, and soaking for 20 minutes to thoroughly sterilize the sample;
(c) soaking for 20 minutes, naturally air drying the taken sample in an aseptic box, cutting with an aseptic scalpel, placing into a clean test tube with the diameter of 15 x 150mm, and plugging the opening of the test tube with a rubber plug to obtain the original ganoderma lucidum strain;
step two, multi-stage culture to obtain cultured ganoderma lucidum strains:
(d) culturing a first-level ganoderma lucidum strain: the first-level ganoderma lucidum strain is cultured by a primary ganoderma lucidum strain and a culture medium, wherein the formula of the culture medium comprises 5g of glucose, 0.5g of royal jelly, 50g of mushroom, 50g of ganoderma lucidum, lg of peptone, 0.3g of beef extract, 2g of agar and 0.5g of NaCl according to the weight part ratio; the preparation method of the culture medium comprises the steps of putting a proper amount of glucose, royal jelly, peptone, beef extract, agar and NaCl into a beaker, adding 350ml of water, stirring uniformly by using a glass rod, and putting the beaker on an asbestos net to be heated to be dissolved; after the medicine is completely dissolved, supplementing water to 400 ml; measuring the PH value of the sample by using a precise PH test paper, and adjusting the pH value of the sample by using an lmol/L HCL solution and an lmol/L NaOH solution to make the pH value of the sample be 7; cleaning Lentinus Edodes and Ganoderma, boiling with water, filtering the boiled liquid, taking out 100ml, mixing with the above culture medium, sterilizing under 1.05kg/cm2Keeping the temperature at 121.3 ℃ for 20 min; when the temperature is reduced to 40 ℃, adding 15ml of the solution into a test tube containing the raw ganoderma lucidum, and carrying out sealed culture for about 30 days to obtain a first-grade ganoderma lucidum strain;
(e) culturing a second-level ganoderma lucidum strain: culturing the second-stage ganoderma lucidum strain by culturing the first-stage ganoderma lucidum strain and a culture medium; the formula of the culture medium for culturing the second-level ganoderma lucidum strain comprises the following components in parts by weight:
1) weighing 10 parts of maca, 10 parts of artificially-planted ginseng, 3 parts of cordyceps militaris, 5 parts of oyster, 5 parts of rhizoma polygonati, 3 parts of ginger, 5 parts of dried orange peel, 0.5 part of cinnamon, 3 parts of Chinese yam, 1 part of liquorice, 1 part of wolfberry fruit, 3 parts of semen cassiae, 3 parts of Chinese date, 0.3 part of bee pollen, 3 parts of mulberry, 1.5 parts of fructus amomi, 0.5 part of longan pulp, 2 parts of endothelium corneum gigeriae galli, 1 part of fingered citron, 1 part of spina date seed and 5 parts of hawthorn, preprocessing, crushing and sieving to obtain raw material powder; adding 4 parts by weight of water into the raw material powder, then adding 5 parts by weight of honey, 5 parts by weight of brown sugar and 10 parts by weight of ganoderma lucidum stock solution, sterilizing, and naturally cooling to obtain a culture medium;
2) putting the culture medium obtained in the step 1) into an aseptic container, adding the rest of the ganoderma lucidum stock solution by weight, uniformly mixing, adding ganoderma lucidum, sealing, carrying out heat preservation cultivation at 30 ℃, starting stirring on the 4 th day, stirring for 2 times per day, continuously stirring for 8 days, stopping stirring, continuously cultivating for 4 months, collecting ganoderma lucidum mycelia to obtain ganoderma lucidum mycelia, and filtering the rest substances in the container to obtain ganoderma lucidum cultivation liquid and ganoderma lucidum cultivation residues;
and thirdly, artificially culturing with the second-level ganoderma lucidum strain to obtain cultured ganoderma lucidum.
Example 2
The ganoderma lucidum oral liquid is prepared from the following raw materials in parts by weight: 2 parts of second-level ganoderma lucidum thallus, 2 parts of ganoderma lucidum culture liquid, 4 parts of ganoderma lucidum culture residues, 100 parts of water and 0.02 part of edible salt. The preparation method comprises the following steps:
adding mature second-level ganoderma lucidum mycelia into 20 parts of water, soaking and filtering to obtain ganoderma lucidum liquid;
adding the rest 80 parts of water into the ganoderma lucidum culture residues, heating to boil, keeping the temperature for 2 hours, filtering, adding the ganoderma lucidum culture solution into the supernatant, and uniformly mixing to obtain ganoderma lucidum mixed solution;
and (3) mixing the ganoderma lucidum liquid obtained in the step (1) and the ganoderma lucidum mixed liquid obtained in the step (2), and adding 0.02 part of edible salt to obtain the ganoderma lucidum oral liquid.
Test examples
150 volunteers were recruited, of which 46 persons aged 30-40 years, 54 persons aged 41-50 years and 50 persons aged 51-60 years; volunteers of each age group were randomly divided into two groups, one group was a test group and the other group was a control group. The ganoderma lucidum oral liquid obtained in example 2 is taken by 30ml of the oral liquid to volunteers of a test group every day for 2 months continuously, and is not taken by volunteers of a control group. The volunteers in the test group and the control group were observed and recorded for sleep, respiratory diseases and other diseases. The results are shown in Table 1.
TABLE 1 test results
As can be seen from the results in table 1, the ganoderma lucidum oral liquid can effectively improve sleep conditions and gastrointestinal functions, regulate blood sugar level, and reduce the prevalence rate of respiratory diseases, thereby improving human immunity, benefiting qi, soothing the nerves, regulating metabolic balance, and strengthening body.
Claims (10)
1. A rapid cultivation method of ganoderma lucidum is characterized by comprising the following steps:
firstly, obtaining a raw ganoderma lucidum strain;
secondly, obtaining cultured ganoderma lucidum strains through multi-stage culture, and dividing the culture into the first-stage ganoderma lucidum strain culture and the second-stage ganoderma lucidum strain culture;
and thirdly, artificially culturing with the second-level ganoderma lucidum strain to obtain cultured ganoderma lucidum.
2. The rapid cultivation method of ganoderma lucidum as claimed in claim 1, wherein the obtaining of the primary ganoderma lucidum strain comprises the following steps:
step 1) selecting first-class native meat ganoderma, cleaning the first-class native meat ganoderma by using clear water, cutting off a part with the thickness of about 3 mm on the surface layer of the first-class native meat ganoderma by using a scalpel subjected to high-temperature disinfection, and cutting a sample of 2 cubic centimeters from the inside of the first-class native meat ganoderma;
step 2) putting the sample into medical alcohol with the concentration of 75%, and immersing the surface of the sample with the alcohol for 20 minutes to thoroughly sterilize the sample;
and 3) after soaking, naturally drying the taken sample in an aseptic box, cutting the sample into pieces by using an aseptic scalpel, putting the pieces into a clean test tube with the size of 15 mm multiplied by 150mm, and plugging the opening of the test tube by using a rubber plug to obtain the primary ganoderma lucidum strain.
3. The rapid cultivation method of ganoderma lucidum as claimed in claim 1, wherein the first-stage ganoderma lucidum strain is cultured by using a primary ganoderma lucidum strain and a culture medium, and the specific process is as follows:
step 1) weighing a proper amount of glucose, royal jelly, peptone, beef extract, agar and NaCl, putting the mixture into a beaker, adding 350ml of water, stirring the mixture uniformly by using a glass rod, putting the mixture on an asbestos net, heating the mixture to dissolve the mixture, and supplementing water to 400ml after the medicine is completely dissolved;
step 2) measuring the pH value of the sample by using a precise pH test paper, and adjusting the pH value of the sample by using an lmol/L HCl solution and an lmol/L NaOH solution to make the pH value of the sample be 7;
step 3) cleaning Lentinus Edodes and Ganoderma, boiling with water, filtering the boiled liquid, taking out 100ml, mixing with the above culture medium, and sterilizing;
and 4) sterilizing, cooling, adding 15ml of solution into a test tube containing the raw ganoderma lucidum when the temperature is reduced to 40 ℃, and carrying out sealed culture for about 30 days to obtain the first-grade ganoderma lucidum strain.
4. The method for rapidly culturing meat ganoderma as claimed in claim 3, wherein the formula of the culture medium for culturing the first-level meat ganoderma species comprises, by weight, 5g of glucose, 0.5g of royal jelly, 50g of shiitake mushroom, 50g of ganoderma lucidum, lg of peptone, 0.3g of beef extract, 2g of agar and 0.5g of NaCl.
5. The rapid culture method of ganoderma lucidum according to claim 3, wherein the sterilization condition is 1.05kg/cm2And maintained at 121.3 deg.c for 20 min.
6. The rapid culture method of ganoderma lucidum according to claim 1, wherein the second-stage ganoderma lucidum strain is cultured by the first-stage ganoderma lucidum strain and a culture medium;
step 1) preparation of raw materials: 8-12 parts of maca, 8-12 parts of artificially-planted ginseng, 0.2-0.5 part of cordyceps militaris, 4-6 parts of oyster, 4-6 parts of rhizoma polygonati, 2-4 parts of ginger, 4-6 parts of dried orange peel, 05-1.5 parts of cinnamon, 2-4 parts of Chinese yam, 0.5-1.5 parts of liquorice, 0.5-1.5 parts of wolfberry, 2-5 parts of semen cassiae, 2-5 parts of Chinese date, 0.1-0.5 part of bee pollen, 2-5 parts of mulberry, 1-2 parts of fructus amomi, 0.3-0.6 part of longan pulp, 1-3 parts of endothelium corneum gigeriae galli, 0.5-2 parts of fingered citron, 0.5-2 parts of spina date seed, 4-6 parts of hawthorn, 6-10 parts of honey and 6-10 parts of brown sugar
Step 2) weighing a proper amount of maca, artificially-planted ginseng, cordyceps militaris, oyster, rhizoma polygonati, ginger, dried orange peel, cinnamon, Chinese yam, liquorice, wolfberry fruit, cassia seed, Chinese date, bee pollen, mulberry, fructus amomi, arillus longan, endothelium corneum gigeriae galli, fingered citron, spina date seed and hawthorn, pretreating, crushing and sieving to obtain raw material powder; adding 3-5 parts by weight of water into the raw material powder, then adding honey, brown sugar and 10 parts of ganoderma lucidum stock solution, sterilizing, and naturally cooling to obtain a culture medium; placing the culture medium into an aseptic container, adding the rest weight part of Ganoderma lucidum stock solution, mixing, adding Ganoderma lucidum, sealing, incubating at 28-33 deg.C, stirring for 3-5 days, stirring for 1-2 times per day, continuously stirring for 5-10 days, stopping stirring, continuously culturing for 3-5 months, and collecting Ganoderma lucidum thallus to obtain Ganoderma lucidum thallus;
and 3) filtering the residual substances in the container to obtain the ganoderma lucidum cultivation liquid and ganoderma lucidum cultivation residues.
7. The rapid cultivation method of ganoderma lucidum as claimed in claim 6, wherein the formula of the culture medium for culturing the second-level ganoderma lucidum strain comprises the following components in parts by weight: 10 parts of maca, 10 parts of artificially-planted ginseng, 3 parts of cordyceps militaris, 5 parts of oyster, 5 parts of rhizoma polygonati, 3 parts of ginger, 5 parts of dried orange peel, 0.5 part of cinnamon, 3 parts of Chinese yam, 1 part of liquorice, 1 part of wolfberry fruit, 3 parts of semen cassiae, 3 parts of Chinese date, 0.3 part of bee pollen, 3 parts of mulberry, 1.5 parts of fructus amomi, 0.5 part of longan pulp, 2 parts of endothelium corneum gigeriae galli, 1 part of fingered citron, 1 part of spina date seed, 5 parts of hawthorn, 5 parts of honey and 5 parts of brown sugar.
8. The ganoderma lucidum oral liquid is characterized by being prepared from the following raw materials in parts by weight: 1-2 parts of second-level ganoderma lucidum thallus, 1-2 parts of ganoderma lucidum culture liquid, 3-5 parts of ganoderma lucidum culture slag and 80-120 parts of water.
9. The meat ganoderma lucidum oral liquid of claim 8, wherein the raw materials further comprise 0.01-0.03 parts of edible salt.
10. A method for preparing the meat ganoderma lucidum oral liquid of claim 1, comprising the following steps:
adding mature second-level ganoderma lucidum mycelia into 10-30 parts of water, soaking, and filtering to obtain ganoderma lucidum liquid;
adding the rest part by weight of water into the ganoderma lucidum culture residues, heating to boil, preserving heat for 1-3h, filtering, adding the supernatant into ganoderma lucidum culture solution, and uniformly mixing to obtain ganoderma lucidum mixed solution;
and (3) mixing the ganoderma lucidum liquid obtained in the step (1) and the ganoderma lucidum mixed liquid obtained in the step (2) to obtain the ganoderma lucidum oral liquid.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113116943A (en) * | 2021-04-27 | 2021-07-16 | 江苏春江生物科技有限公司 | Preparation method and device for treating blood sugar by fermenting Chinese herbal medicines for the aged |
| CN113951051A (en) * | 2021-10-14 | 2022-01-21 | 重庆泰宜生物科技有限责任公司 | Preparation process for improving PQQ content of artificially-cultured ganoderma lucidum |
| CN114766515A (en) * | 2022-05-13 | 2022-07-22 | 地宝集团有限公司 | Ganoderma lucidum breeding stabilizer and application method thereof |
| CN115886242A (en) * | 2022-11-23 | 2023-04-04 | 河北岁神本草肉灵芝生物科技有限公司 | Ganoderma lucidum, high-concentration oral liquid containing ganoderma lucidum and preparation method of oral liquid |
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| CN108112422A (en) * | 2017-12-20 | 2018-06-05 | 河北岁神本草肉灵芝生物科技有限公司 | The breeding method of meat ganoderma lucidum, oral liquid comprising meat ganoderma lucidum and preparation method thereof |
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| CN113116943A (en) * | 2021-04-27 | 2021-07-16 | 江苏春江生物科技有限公司 | Preparation method and device for treating blood sugar by fermenting Chinese herbal medicines for the aged |
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| CN114766515A (en) * | 2022-05-13 | 2022-07-22 | 地宝集团有限公司 | Ganoderma lucidum breeding stabilizer and application method thereof |
| CN115886242A (en) * | 2022-11-23 | 2023-04-04 | 河北岁神本草肉灵芝生物科技有限公司 | Ganoderma lucidum, high-concentration oral liquid containing ganoderma lucidum and preparation method of oral liquid |
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