CN114766515A - Ganoderma lucidum breeding stabilizer and application method thereof - Google Patents
Ganoderma lucidum breeding stabilizer and application method thereof Download PDFInfo
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Images
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/44—Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
Abstract
The invention discloses a ganoderma lucidum breeding stabilizer and a using method thereof, and the ganoderma lucidum breeding stabilizer comprises the following steps: step S1, performing gene extraction on the ganoderma lucidum composite flora; step S2, separating ganoderma lucidum slime from ganoderma lucidum composite flora; step S3, preparing a couch grass and snow lotus compound solution; step S4, adding myxobacteria of ganoderma lucidum into the composite solution for fermentation culture; step S5, centrifuging the fermentation liquor to obtain supernatant; and step S6, concentrating the supernatant, and freeze-drying to obtain the finished product ganoderma lucidum breeding stabilizer. The ganoderma lucidum breeding stabilizer and the use method thereof utilize the gene extraction device to extract the compound flora from the original ganoderma lucidum, the whole process is closed, the influence of manual operation on the flora is reduced, the ganoderma lucidum slime is utilized to be fermented and cultured together with the compound solution of the couch grass and the snow lotus, the couch grass has stress resistance, and the snow lotus has cold resistance, so that the ganoderma lucidum breeding stabilizer with the stabilizing effect is prepared, and the stabilizer is added inwards when plants are cultivated, so that the original resistance of the plants is recovered.
Description
Technical Field
The invention relates to the technical field related to ganoderma lucidum cultivation, in particular to a ganoderma lucidum breeding stabilizer and a use method thereof.
Background
Taisui, also called meat ganoderma lucidum, Li Shizhen's book of compendium of materia Medica: meat sesame like meat. It is also attached to the large stone, with the head and tail being biological. Red such as coral, white such as fat, black such as sun euphorbia, green such as jade feather, yellow such as purple gold, all of which are all bright holes such as ice, ganoderma lucidum is a rare polymer composed of three types of bacteria such as slime mold, bacteria and fungi, and is the oldest ancient living organism specimen discovered so far.
Researches show that the plant can not completely transmit the original genetic gene in the long growth and evolution process, has variation and partial memory loss, gradually degrades the original natural instinct of cold resistance, heat resistance, disease and insect resistance and the like of the plant, and particularly, the artificially cultivated plant basically loses the original wild attribute of the plant and leads the plant to generate serious dependence and quality degradation variation on the environment and human beings.
Disclosure of Invention
The invention provides a ganoderma lucidum breeding stabilizer and a using method thereof in order to make up for market gaps.
The invention aims to provide a ganoderma lucidum breeding stabilizer and a using method thereof, which aim to solve the problems that the original wild property of a plant is basically lost and the plant generates serious dependence on the environment and human beings and quality degradation variation in artificially cultivated plants, which are proposed in the background art.
In order to achieve the purpose, the invention provides the following technical scheme: a ganoderma lucidum breeding stabilizer comprises the following steps:
step S1, performing gene extraction on the ganoderma lucidum composite flora;
step S2, separating ganoderma lucidum slime from ganoderma lucidum composite flora;
step S3, preparing a couch grass and snow lotus composite solution;
step S4, adding the ganoderma lucidum slime into a composite solution for fermentation culture:
adding nutrient solution and Ganoderma lucidum slime into the composite solution, and culturing at 24-28 deg.C for 30-40 hr;
step S5, centrifuging the fermentation liquor to obtain supernatant;
and step S6, concentrating the supernatant, and freeze-drying to obtain the finished product ganoderma lucidum breeding stabilizer.
A use method of a ganoderma lucidum breeding stabilizer comprises the following steps:
adding a proper amount of ganoderma lucidum breeding stabilizer into cold water to be diluted to 5g/L, heating the diluted stabilizer solution to 30 ℃ in a water-proof manner, heating for 20 minutes, uniformly mixing the stabilizer solution with plant seeds or plant cell tissues to obtain a plant mixed solution, carrying out staged cooling adaptation on the plant mixed solution to minus 60 ℃, then carrying out staged heating adaptation on the plant mixed solution to a modern normal temperature state, taking out and culturing the plant seeds or plant cell tissues, and spraying the stabilizer solution once on leaves in the seedling stage, early flowering stage and post-flowering and granulation stage of plants.
Further, step S1 includes the steps of:
cleaning: cleaning the original ganoderma lucidum in a cleaning tank, and lifting the cleaned original ganoderma lucidum upwards by a filter plate;
and (3) disinfection: pushing the original meat lucid ganoderma into a chopping tank by a push plate through a spray head, spraying disinfectant water to the original meat lucid ganoderma by the spray head, and then spraying sterile water;
slitting: the cutting knife cuts the original ganoderma lucidum falling into the cutting groove, and the conveying belt conveys the cut ganoderma lucidum to the grinding groove;
grinding and diluting: grinding the crushed ganoderma lucidum falling into the grinding groove by a grinding wheel, and injecting sterile water into the grinding groove by a liquid adding pipe to obtain ganoderma lucidum solution;
shaking up: enabling the ganoderma lucidum solution to flow into a shaking box for shaking, and conveying the shaken ganoderma lucidum solution to a moving box through a peristaltic pump;
and (3) cultivating: the second screw rod inoculates the ganoderma lucidum solution in a culture container for culture.
Further, step S2 is to screen the flora growing in the culture container and separate the mythic fungus of ganoderma lucidum, step S4 is to wash, disinfect, cut, grind and dilute the thatch and the snow lotus respectively, then add a cracking agent into the diluent to soak, and after soaking for 2 hours, sterilize and mix to obtain a composite solution.
Further, the gene extraction device comprises a box body, a control panel, a first liquid storage tank and a second liquid storage tank are installed on the top surface of the box body, the control panel, the first liquid storage tank and the second liquid storage tank are respectively connected with a controller, a liquid adding pipe and a spray header, and a first partition plate is fixed on the inner wall of the middle of the box body.
Further, the top of washing tank covers there is the apron, the opening has been seted up on the posterior lateral plate of washing tank, opening intercommunication washing tank and cut up the groove, the telescopic link is connected to the bottom surface of filter plate, the telescopic link is fixed on first baffle, the push pedal is in the same place with first screw rod spiro union, first screw rod runs through the opening and connects the front and back curb plate of box.
Further, the shredding tank is located the washing tank rear side, install the branch cutter directly over the shredding tank on the box, the bottom of shredding tank is provided with the conveyer belt of carrying right.
Further, grind the inside in groove and placed the rolling wheel, the rolling wheel rotates and connects first driver, first driver is fixed on the preceding curb plate of box, grind the right side in groove and install and shake even box, shake even box slidable mounting on the guide rail, the guide rail is fixed on first baffle, the preceding curb plate that shakes even box links to each other with the second driver.
Further, install the hose in the peristaltic pump, the both ends of hose are inserted respectively and are shaken even box and removal box, install inoculator and discharge tube respectively on the front and back both sides face of removal box, welded fastening has the company's board on the posterior lateral plate of removal box, even board and second screw rod spiro union are in the same place, lateral wall about the box is connected at the both ends of second screw rod.
Furthermore, the culture containers are placed on a second partition board at equal intervals, the rear end of the second partition board is connected with a third partition board, the third partition board is fixed on a bottom board of the box body, and the second partition board, the third partition board and the bottom board of the box body surround to form a constant-temperature water bath.
Compared with the prior art, the invention has the beneficial effects that: this meat glossy ganoderma breeding stabilizer and application method thereof, utilize the gene extraction device to extract compound flora from native meat glossy ganoderma, whole process is sealed and is gone on, it influences the flora to reduce manual operation, utilize meat glossy ganoderma slime fungus and cogongrass and saussurea involucrate composite solution fermentation culture together, the cogongrass has the adversity, the saussurea involucrate has cold resistance, thereby make the meat glossy ganoderma breeding stabilizer that has stable effect, inwards add the stabilizer when cultivating the plant, make the plant resume original attribute, have characteristics such as cold-resistant simultaneously, avoid traditional breeding to lead to plant transition dependence and quality degradation variation.
Drawings
FIG. 1 is a schematic top view of a gene extraction apparatus according to the present invention;
FIG. 2 is a schematic sectional top view of the gene extracting apparatus of the present invention;
FIG. 3 is a schematic front sectional view of the gene extracting apparatus of the present invention;
FIG. 4 is a schematic sectional side view of the gene extracting apparatus of the present invention.
In the figure: 1. a box body; 2. a cleaning tank; 3. a shredding tank; 4. a grinding groove; 5. shaking up the box; 6. a peristaltic pump; 7. moving the cartridge; 8. a culture vessel; 9. a constant temperature water bath; 11. controlling the panel; 12. a first liquid storage tank; 13. a second liquid storage tank; 14. a first separator; 15. a controller; 21. a cover plate; 22. filtering the plate; 23. a telescopic rod; 24. pushing the plate; 25. a first screw; 26. a shower head; 27. an opening; 31. a slitting knife; 32. a conveyor belt; 41. a rolling wheel; 42. a first driver; 43. a liquid feeding pipe; 51. a second driver; 52. a guide rail; 61. a hose; 71. a second screw; 72. an inoculator; 73. a discharge pipe; 74. connecting plates; 91. a second separator; 92. and a third partition plate.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
The first embodiment is as follows: referring to fig. 1-4, the present invention provides a technical solution: a ganoderma lucidum breeding stabilizer comprises the following steps:
step S1, performing gene extraction on the ganoderma lucidum composite flora;
step S2, separating ganoderma lucidum slime from ganoderma lucidum complex flora;
step S3, preparing a couch grass and snow lotus composite solution;
step S4, adding myxobacteria of ganoderma lucidum into the composite solution for fermentation culture:
adding nutrient solution and myxobacteria of Ganoderma lucidum into the composite solution, and culturing at 24-28 deg.C for 30-40 hr;
step S5, centrifuging the fermentation liquor to obtain supernatant;
step S6, concentrating the supernatant, and freeze-drying to obtain the finished product ganoderma lucidum breeding stabilizer;
freezing the concentrated supernatant at step S6 helps to extend the shelf life of the stabilizer and reduce loss of the active ingredient.
The second embodiment is as follows: a use method of a ganoderma lucidum breeding stabilizer comprises the following steps:
adding cold water into a proper amount of ganoderma lucidum breeding stabilizer to dilute the ganoderma lucidum breeding stabilizer to 5g/L, heating the diluted stabilizer solution to 30 ℃ in a water-proof manner, heating the stabilizer solution for 20 minutes, uniformly mixing the stabilizer solution with plant seeds or plant cell tissues to obtain a plant mixed solution, carrying out staged cooling adaptation on the plant mixed solution to minus 60 ℃, then carrying out staged heating adaptation on the plant mixed solution to a modern normal temperature state, taking out the extracted plant seeds or plant cell tissues for cultivation, and spraying the stabilizer solution once on the plant seeds or plant cell tissues in a seedling stage, a prophase and a post-blooming stage.
The traditional Chinese medicine components in the stabilizer are gradually released by adding a heat stabilizer solution through water isolation, the activity of effective components is improved, the myxobacter taisuis breeding stabilizer utilizes the special properties of restoration, ancient recovery and rotation of the myxobacter taisuis to place plant seeds or plant cell tissues in the special environment of the myxobacter taisuis, so that the plant seeds or the plant cell tissues are subjected to the glacier-like cycle survival conditions and environment again under the protection of the myxobacter taisuis, the original properties of the plant are restored by the stress reaction of natural adaptation freezing, hibernation, unfreezing, reawakening and growth of the plant seeds or the plant cell tissues, the myxobacter taisuis guides the plant seeds to restore the original memory, original resistance and original high-yield high properties of the plant, and the traditional breeding mode is broken through.
Finds and masters the corresponding mechanism of the plant chromosome repair enzyme to the growth environment in the growth and life continuation of the plant, makes full use of the stress response of the plant to preserve the life in the glacier period and promotes the flash point of the plant evolution.
The third concrete implementation mode: as shown in fig. 2 and 3, step S1 includes the following steps:
cleaning: cleaning the original ganoderma lucidum in the cleaning tank 2, and lifting the cleaned original ganoderma lucidum by the filter plate 22;
and (3) disinfection: the push plate 24 pushes the original meat ganoderma lucidum to enter the chopping tank 3 through the spray head 26, and the spray head 26 sprays sterile water to the original meat ganoderma lucidum firstly and then sprays sterile water;
slitting: the cutting knife 31 cuts the original ganoderma lucidum falling into the cutting groove 3, and the conveyor belt 32 conveys the cut ganoderma lucidum to the grinding groove 4;
grinding and diluting: grinding wheel 41 grinds the crushed ganoderma lucidum falling into grinding groove 4, and liquid adding pipe 43 injects sterile water into grinding groove 4 to obtain ganoderma lucidum solution;
shaking up: enabling the ganoderma lucidum solution to flow into a shaking box 5 for shaking, and conveying the shaken ganoderma lucidum solution to a moving box 7 through a peristaltic pump 6;
culturing: the second screw 71 inoculates the ganoderma lucidum solution in the culture vessel 8 for culture.
However, after the worker puts the original meat ganoderma lucidum into the cleaning tank 2, the cover plate 21 is covered, and then the procedures of cleaning, disinfecting, cutting, grinding, diluting, shaking and cultivating are automatically carried out by the gene extraction device, so that the contact between the outside and the extracted flora is reduced, and the interference of mixed bacteria is avoided.
The fourth concrete implementation mode: the embodiment is further limited to the first embodiment, in which step S2 is to screen the flora growing in the culture container 8 and separate the myxobacteria of ganoderma lucidum, and step S4 is to wash, disinfect, cut, grind and dilute the thatch and saussurea involucrate, respectively, add a lysis agent into the diluent to soak the thatch and the saussurea involucrate, and sterilize and mix the mixture after soaking for 2 hours to obtain a composite solution.
In step S4, the gene extraction device is used for the couch grass and saussurea involucrate, after dilution, the couch grass and saussurea involucrate diluent is pumped into the movable box 7 by the peristaltic pump 6, and the movable box 7 is directly discharged outwards through the discharge pipe 73.
The couch grass and the snow lotus in the step S4 can be replaced by other Chinese herbal medicines, the adjustment is carried out according to the requirement of the cultivated plants, when the cold resistance of the cultivated plants is realized, the Chinese herbal medicines with the cold resistance effect are used, and when the drought resistance of the cultivated plants is realized, the Chinese herbal medicines with the heat resistance effect are used. During cultivation, the stabilizer is added inwards, so that the plants are restored to be original high and high in yield, seeds are not degenerated, and the yield can be increased by 5% every year.
The fifth concrete implementation mode: the present embodiment is a further limitation of the first specific embodiment, as shown in fig. 1, 2 and 3, the gene extracting apparatus includes a box 1, a control panel 11, a first liquid storage tank 12 and a second liquid storage tank 13 are installed on a top surface of the box 1, the control panel 11, the first liquid storage tank 12 and the second liquid storage tank 13 are respectively connected to a controller 15, a liquid adding pipe 43 and a spray header 26, a first partition plate 14 is fixed on an inner wall of a middle portion of the box 1, solutions in the first liquid storage tank 12 and the second liquid storage tank 13 are adjusted as required, during sterilization, sterile water is injected into the first liquid storage tank 12 first, the spray header 26 sprays sterile water to the ganoderma lucidum, and then sterile water is injected into the first liquid storage tank 12, so that the spray header 26 sprays the sterile water to flush the ganoderma lucidum.
The sixth specific implementation mode: this embodiment is the third further limited of embodiment, the top of washing tank 2 covers there is apron 21, opening 27 has been seted up on the posterior lateral plate of washing tank 2, opening 27 intercommunication washing tank 2 and shredding tank 3, telescopic link 23 is connected to the bottom surface of filter plate 22, telescopic link 23 fixes on first baffle 14, push pedal 24 is in the same place with first screw rod 25 spiro union, first screw rod 25 runs through opening 27 and connects the anterior posterior lateral plate of box 1, telescopic link 23 upwards lifts filter plate 22 to opening 27 height, push pedal 24 offsets with filter plate 22 this moment, first screw rod 25 rotates and makes push pedal 24 be the motion of sharp slip table, propelling movement meat glossy ganoderma is to 3 direction movements in shredding tank.
The seventh embodiment: the third specific embodiment further defines that the shredding tank 3 is located at the rear side of the cleaning tank 2, a dividing cutter 31 is installed on the box body 1 right above the shredding tank 3, a conveyor belt 32 which is conveyed rightwards is arranged at the bottom of the shredding tank 3, and a lifting mechanism is connected to the dividing cutter 31, so that a cutting effect is achieved.
The specific implementation mode eight: in the third embodiment, as shown in fig. 3 and 4, a rolling wheel 41 is placed inside the grinding trough 4, the rolling wheel 41 is rotatably connected with a first driver 42, the first driver 42 is fixed on the front side plate of the box 1, a shaking box 5 is installed on the right side of the grinding trough 4, the shaking box 5 is slidably installed on a guide rail 52, the guide rail 52 is fixed on the first partition 14, and the front side plate of the shaking box 5 is connected with a second driver 51.
The specific implementation method nine: as shown in fig. 2 and 3, a hose 61 is installed in the peristaltic pump 6, two ends of the hose 61 are respectively inserted into the shaking box 5 and the moving box 7, an inoculator 72 and a discharge pipe 73 are respectively installed on the front side and the rear side of the moving box 7, a connecting plate 74 is welded and fixed on the rear side plate of the moving box 7, the connecting plate 74 is screwed with a second screw 71, two ends of the second screw 71 are connected with the left side wall and the right side wall of the box body 1, the second screw 71 rotates to enable the connecting plate 74 to drive the moving box 7 to move left and right, the inoculator 72 is enabled to move right above the culture container 8, and a driver of the second screw 71 is not shown in the drawing.
The detailed implementation mode is ten: this embodiment is a further limitation of the third embodiment, as shown in fig. 3 and 4, the culture containers 8 are placed on the second partition plate 91 at equal intervals, the rear end of the second partition plate 91 is connected with the third partition plate 92, the third partition plate 92 is fixed on the bottom plate of the box body 1, the second partition plate 91, the third partition plate 92 and the bottom plate of the box body 1 surround to form the constant temperature water bath 9, the culture medium is placed in the culture container 8, the top end of the culture container 8 is covered with a film, when inoculating the strain, the inoculator 72 penetrates the film downwards to inject the strain on the culture medium, and the constant temperature water bath 9 is used for providing proper temperature for culture.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. The ganoderma lucidum breeding stabilizer is characterized by comprising the following steps:
step S1, performing gene extraction on the ganoderma lucidum composite flora;
step S2, separating ganoderma lucidum slime from ganoderma lucidum complex flora;
step S3, preparing a couch grass and snow lotus composite solution;
step S4, adding the ganoderma lucidum slime into a composite solution for fermentation culture:
adding nutrient solution and Ganoderma lucidum slime into the composite solution, and culturing at 24-28 deg.C for 30-40 hr;
step S5, centrifuging the fermentation liquor to obtain supernatant;
and step S6, concentrating the supernatant, and freeze-drying to obtain the finished product ganoderma lucidum breeding stabilizer.
2. The use method of the ganoderma lucidum breeding stabilizer is characterized by comprising the following steps: the method comprises the following steps:
adding cold water into a proper amount of ganoderma lucidum breeding stabilizer to dilute the ganoderma lucidum breeding stabilizer to 5g/L, heating the diluted stabilizer solution to 30 ℃ in a water-proof manner, heating the stabilizer solution for 20 minutes, uniformly mixing the stabilizer solution with plant seeds or plant cell tissues to obtain a plant mixed solution, carrying out staged cooling adaptation on the plant mixed solution to minus 60 ℃, then carrying out staged heating adaptation on the plant mixed solution to a modern normal temperature state, taking out the extracted plant seeds or plant cell tissues for cultivation, and spraying the stabilizer solution once on the plant seeds or plant cell tissues in a seedling stage, a prophase and a post-blooming stage.
3. The ganoderma lucidum breeding stabilizer according to claim 1, wherein the step S1 comprises the following steps:
cleaning: putting the original ganoderma lucidum into a cleaning tank (2) for cleaning, and lifting the cleaned original ganoderma lucidum upwards by a filter plate (22);
and (3) disinfection: the pushing plate (24) pushes the original meat ganoderma lucidum to enter the chopping tank (3) through the spray head (26), and the spray head (26) sprays sterile water to the original meat ganoderma lucidum firstly and then sprays sterile water;
slitting: the cutting knife (31) cuts the original ganoderma lucidum falling into the cutting groove (3), and the conveyor belt (32) conveys the cut ganoderma lucidum to the grinding groove (4);
grinding and diluting: grinding wheel (41) grinds the crushed ganoderma lucidum falling into the grinding groove (4), and liquid adding pipe (43) injects sterile water into the grinding groove (4) to obtain ganoderma lucidum solution;
shaking up: enabling the ganoderma lucidum solution to flow into the shaking box (5) for shaking, and conveying the shaken ganoderma lucidum solution to the moving box (7) through the peristaltic pump (6);
and (3) cultivating: the second screw (71) inoculates the ganoderma lucidum solution in the culture container (8) for culture.
4. The ganoderma lucidum breeding stabilizer according to claim 1, wherein the stabilizer comprises: and S2, screening floras growing in the culture container (8) and separating the mythic fungus of the ganoderma lucidum, wherein S4 is to respectively wash, disinfect, cut, grind and dilute the thatch and the snow lotus, add a cracking agent into the diluent for soaking, and sterilize and mix the diluent for 2 hours to obtain a composite solution.
5. The ganoderma lucidum breeding stabilizer according to claim 1, which is characterized in that: the gene extraction device comprises a box body (1), a control panel (11), a first liquid storage tank (12) and a second liquid storage tank (13) are installed on the top surface of the box body (1), the control panel (11), the first liquid storage tank (12) and the second liquid storage tank (13) are respectively connected with a controller (15), a liquid adding pipe (43) and a spray header (26), and a first partition plate (14) is fixed on the inner wall of the middle of the box body (1).
6. The ganoderma lucidum breeding stabilizer according to claim 3, wherein the stabilizer comprises: the top of washing tank (2) covers there is apron (21), opening (27) have been seted up on the posterior lateral plate of washing tank (2), opening (27) intercommunication washing tank (2) and cut up groove (3), telescopic link (23) are connected to the bottom surface of filter plate (22), telescopic link (23) are fixed on first baffle (14), push pedal (24) are in the same place with first screw rod (25) spiro union, curb plate around opening (27) connection box (1) is run through in first screw rod (25).
7. The ganoderma lucidum breeding stabilizer and the use method thereof according to claim 3, wherein the stabilizer comprises the following components in percentage by weight: cut up groove (3) and be located washing tank (2) rear side, install on box (1) directly over cut up groove (3) and divide cutter (31), the bottom of cut up groove (3) is provided with conveyer belt (32) of carrying right.
8. The ganoderma lucidum breeding stabilizer according to claim 3, wherein the stabilizer comprises the following components in percentage by weight: grind inside in groove (4) and placed rolling wheel (41), rolling wheel (41) are rotated and are connected first driver (42), first driver (42) are fixed on the preceding curb plate of box (1), grind the right side in groove (4) and install and shake even box (5), shake even box (5) slidable mounting on guide rail (52), guide rail (52) are fixed on first baffle (14), the preceding curb plate of shaking even box (5) links to each other with second driver (51).
9. The ganoderma lucidum breeding stabilizer according to claim 3, wherein the stabilizer comprises the following components in percentage by weight: install hose (61) in peristaltic pump (6), the both ends of hose (61) are inserted respectively and are shaken even box (5) and removal box (7), install inoculator (72) and discharge pipe (73) on the front and back both sides face of removal box (7) respectively, welded fastening has even board (74) on the posterior lateral plate of removal box (7), even board (74) and second screw rod (71) spiro union are in the same place, lateral wall about box (1) is connected at the both ends of second screw rod (71).
10. The ganoderma lucidum breeding stabilizer according to claim 3, wherein the stabilizer comprises the following components in percentage by weight: the culture containers (8) are placed on the second partition plates (91) at equal intervals, the rear ends of the second partition plates (91) are connected with the third partition plates (92), the third partition plates (92) are fixed on the bottom plate of the box body (1), and the second partition plates (91), the third partition plates (92) and the bottom plate of the box body (1) surround to form a constant-temperature water bath (9).
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Cited By (1)
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| CN116042403A (en) * | 2023-02-06 | 2023-05-02 | 地宝集团有限公司 | Meat ganoderma lucidum breeding stabilizer and using method thereof |
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