TWM505174U - Cultivation device for tissue culture of Saussurea involucrata - Google Patents

Description

Saussurea tissue culture culture device

The present invention relates to a culture culture device, and more particularly to an innovative structural type revealer for collecting snow lotus tissue culture.

According to the snow lotus, Saussureu involucrata Kar.et Kin, also known as snow lotus, is a perennial herb and is the only large herb that can grow on the snow line. Snow lotus takes 6-8 years from seed germination to bolting and flowering, and flowers from June to August in the last year. The special growth environment makes it natural and rare, and it creates its unique and magical pharmacological effects. According to the analysis of modern chemical composition, Xuelian whole grass contains alkaloids, flavonoids, simple alcohols, volatile oils, reducing sugars and sixteen kinds of amino acids, Xuelian vinegar, etc. The main functions are cold dehumidification, pain relief, Huoxuetongjing, Qigong San痪, strong gluten to help the yang, promote the function of the contractor contraction. Flavonoids, polysaccharides and sesquiterpenoids are associated with various pharmacological activities and have great potential for development in the treatment of rheumatic pain, prevention of cardiovascular disease, anti-aging, anti-cancer and family planning. In recent years, the research on the pharmacological effects of snow lotus against rheumatism, analgesia, cardiotonic, anti-cancer, antihypertensive, antiasthmatic, termination of pregnancy, scavenging free radicals and anti-fatigue has made gratifying progress, and it is expected to develop into a new class of drugs for prevention and treatment of rheumatic pain. . Modern research has shown that it mainly contains polyhydroxyflavonoids, which have obvious anti-inflammatory and analgesic effects.

However, due to the long-term growth and long-term piracy of Xuelian, the number has gradually become rare and has been identified as the national second-class endangered plant by China. Therefore, the wild snow lotus is rare, so it is cultivated by artificial cultivation. The snow lotus began to be valued by people and gradually accepted.

However, since the technique of artificially cultivating snow lotus is still in its infancy, in the process of exploration, it is inevitable that there are various unexplained reasons leading to low yield, or the extraction technology is not mature enough to make the effect insufficient, and the consumer's willingness to purchase is reduced.

Therefore, in view of the problems existing in the above-mentioned conventional structure, how to develop an innovative structure of a more ideal and practical Saussurea tissue culture culture device, which is desired by the users, is also the goal of the relevant industry to strive to develop breakthroughs and direction.

In view of this, the creator has been engaged in the manufacturing development and design experience of related products for many years. After the detailed design and careful evaluation of the above objectives, the creator will have a practical and practical creation.

The main purpose of this creation is to provide a plant tissue culture culture device, and the technical problem to be solved is to develop a new type of snow lotus tissue culture culture which is more ideal and practical and can improve the active ingredient of the culture. The device is an innovative breakthrough for the goal.

The technical feature of the present problem solving problem is mainly that the snow lotus tissue culture culture device comprises a light-transparent sterilization tank, a slicing device installed above the light-transmitting sterilization tank, and a transport device connected to the slicing device. a plant breeding device, a first conveying device connected to the top of the cutting device and the transparent sterilization tank, a plant culture device installed at the bottom of the transparent sterilization tank, and a second conveying device connected to the plant culture device a callus screening device and a central processing unit, the central processing unit being electrically connected to the slicing device, the first transport device, the second transport device, and the callus screening device, respectively, thereby controlling the timing of each of the devices After the breeding is completed, the snow lotus seedlings are sliced by the slicing device, and then transported to the plant culture device through the first conveying device to cultivate the callus of the snow lotus, and the callus is transported to the callus through the second conveying device. After the device is screened, it is collected as a cell mass for subsequent use.

With this innovative and unique design, this creation can be obtained through the medium culture method through the medium culture method, and the temperature and humidity control means can obtain the callus containing the snow lotus, and the concentration of the active ingredient in the callus It can be controlled by the number of times of cultivation and collection, and can be provided for different purposes, without the problem of insufficient quality, which will be insufficient effective ingredients, ensuring the expected effect after use, achieving practical progress and better industrial economy. (utilization) benefits.

The principle utilized in this creation is a plant cell culture technique. Based on the pluripotency of plant cells, any plant cell with intact nuclei has all the genetic information necessary for the formation of a complete plant and the ability to develop into a complete plant. Therefore, plant cell culture technology is a safe use of the power from the plant itself. technology. Plant cell culture technology is a high-tech developed in the international market in recent years. It is also an internationally-recognized mature biotechnology. It mainly inoculates isolated plant cells on artificially configured medium under aseptic conditions. A technique for obtaining a large number of cell populations by artificially controlling the growth environment and conditions for large-scale cultivation and mass-producing cells. The structure related to the creation of the technology can be roughly divided into steps of seed selection, seedling raising, slice culture, inducing callus and collecting high quality callus. The following is a detailed description of the above-mentioned culture collection process with the structure and schema of the creation.

Please refer to Fig. 1 for a preferred embodiment of the present invention for the preparation of the Saussurea tissue culture apparatus, but these examples are for illustrative purposes only and are not limited by this structure in the patent application. The snow lotus tissue culture culture device comprises a light-transmissive sterilization tank 10, a slicing device 30 connected to the light-transmitting sterilization tank 10, a plant breeding device 20 connected to the slicing device 30, and a slicing device connected thereto. 30. The first conveying device 40 at the top of the transparent sterilization tank 10, a plant culture device 50 installed at the bottom of the transparent sterilization tank 10, and a callus tissue connected to the plant cultivation device 50 by the second conveying device 60 The screening device 70 and a central processing unit 80 are electrically connected to the slicing device 30, the first transport device 40, the second transport device 60, and the callus screening device 70, respectively, thereby controlling the actuation thereof. opportunity.

The bottom of the transparent sterilizing tank 10 is formed with at least one groove 11. In order to reduce the cost and increase the productivity, the following description is made by taking the embodiment of the plurality of grooves 11 as an example; A plurality of perforations 12 are formed, wherein each of the perforations 12 corresponds vertically to a groove 11 respectively. In the preferred embodiment of the present invention, in order to facilitate the control of the temperature and humidity in the transparent sterilization tank 10, the transparent sterilization tank 10 is further provided with a humidity control device 13 and a temperature control device 14, the humidity control The device 13 and the temperature control device 14 are respectively provided with a humidity sensor 15 and a temperature sensor 16 after extending into the transparent sterilization tank 10. The humidity control device 13 and the temperature control device 14 are electrically connected to the central processing unit 80, respectively, thereby controlling the operation time of the humidity control device 13 and the temperature control device 14, thereby illuminating the transparent sterilization tank 10. The temperature and humidity are controlled in an environment suitable for cultivating the plant cells of the snow lotus, and the effect of increasing the productivity is achieved. Further, the humidity control device 13 and the temperature control device 14 can be further extended into the plant breeding device 20, thereby controlling the temperature and humidity in the plant breeding device 20 in an environment most suitable for the growth of the snow lotus seedlings.

The first delivery device 40 includes a main delivery tube 41 and a plurality of manifolds 42 in communication with the main delivery tube 40, wherein the free ends of each manifold 42 are received in a corresponding perforation 12.

The plant culture device 50 includes a plurality of petri dishes 51, wherein each of the culture dishes 51 is placed in a corresponding recess 11 and vertically corresponds to a manifold 42; each of the culture dishes 51 The medium is accommodated, and the medium is mainly in the process of tissue culture of the snow lotus, and the active ingredients are induced therein.

With the above-mentioned structural composition design, the following is the description of the use of this creation: First, we must first confirm the source of the high-quality Tianshan Snow Lotus through the certification process, and then use the structure revealed by this creation to carry out the process of cultivation and collection. Get the callus of Xuelian, which is rich in active ingredients. Please also refer to the first and second figures. After confirming the provenance, firstly, a large number of snow lotus seedlings are cultivated by the plant breeding device 20. In the process of breeding the snow lotus seedlings, the humidity control device 13 and the temperature control device can be fully utilized. 14 Control the cultivation environment of snow lotus seedlings, in order to obtain the maximum quantity of high quality snow lotus seedlings in the shortest time; after the snow lotus seedlings grow to a certain extent, each petri dish 12 pre-filled with plant culture medium is placed into a corresponding concave In the tank 11, the breeding lotus seedlings are transported to the slicing device 30 by a transport device 21 installed between the plant breeding device 20 and the slicing device 30, and the slicing is carried out after slicing. The first conveying device 40, with its main conveying pipe 41 and each manifold 42, distributes the sliced young lotus stems evenly in each of the culture dishes 51, and then uses the plant medium in the culture dish 51 to induce an effective ingredient. Snow lotus callus, and in the process of inducing snow lotus callus, the humidity control device 13 and the temperature control device 14 can be fully utilized to control the snow lotus callus Weaving induces the environment, in order to obtain more high-quality (including more active ingredients) of snow lotus callus. Finally, the substance that completes the inducing procedure is transported to the callus screening device 70 by the second transport device 60 to remove the inferior callus and residual impurities, select the high quality callus of the snow lotus, and finally collect the cells as a cell mass. use.

The above description is described by taking an inducing procedure as an example. Of course, it is also possible to repeat the actions of tissue culture and screening a plurality of times, because each culture program can use the components of the medium to induce more active ingredients, and therefore, after multiple cultivation and The content of the active ingredients of the snow lotus callus obtained after the screening procedure is higher, and of course the effect after use is more significant.

Efficacy Description: The "Snow Lotus Tissue Culture Apparatus" disclosed in the present invention mainly obtains a callus containing snow lotus by the culture method of the permeate medium and the control method of temperature and humidity, and the callus is obtained. The concentration of the active ingredient can be controlled by the number of times of cultivation and collection, and can be provided to different uses without the problem of excessive quality or insufficient active ingredients, and the expected effect after use can be ensured. Furthermore, the use of the Saussurea callus culture produced by this creation has the following product advantages. 1. Guarantee genetic advantage: The best wild Tianshan snow lotus seed is selected before breeding, and the genetic advantage of the gene can be ensured through uninterrupted cell screening. 2, stable quality: strict control of culture conditions and environment, with a good living environment and sufficient nutrients, that is, to ensure the stable quality of plant cells. 3. High content of active ingredients: After thousands of screenings, tests, identification, cultivation, and re-screening, the active ingredients of callus cultivated are basically the same as or even higher than wild snow lotus. 4. There will be no species degradation: through uninterrupted seed rejuvenation procedures (live germplasm banks, liquid nitrogen storage banks, repeated screening) to ensure that species are not degraded. 5. There is no pesticide residue problem: it is cultivated in a sterile environment without pests and pathogens, so there is no need to use any pesticides. Relatively, of course, there will be no problem of pesticide residues.

The disclosures of the above embodiments are used to specifically explain the present creation, and although the descriptions are made through specific terms, the scope of patents of the novel creation cannot be limited thereby; those skilled in the art can understand the spirit of the creation. Changes and modifications are made to the equivalent purpose, and such changes and modifications are to be included in the scope defined by the scope of the patent application as described later.

10‧‧‧Light-transfer sterilization tank 11‧‧‧ Groove 12‧‧‧Perforation 13‧‧‧Humidity control device 14‧‧‧ Temperature control device 15‧‧‧Humidity sensor 16‧‧‧ Temperature sensor 20‧‧ ‧ Plant Breeding Device 21‧‧‧Transporting Device 30‧‧‧Slicing Device 40‧‧‧First Conveying Device 41‧‧‧Main Conveying Tube 42‧‧‧Management 50‧‧‧ Plant Culture Device 51‧‧‧ Petri Dish 60‧‧‧Second transport device 70‧‧‧ callus screening device 80‧‧‧ central processing unit

Figure 1 is a schematic plan view of the creation. The second picture is a schematic diagram of the cultivation of the callus of snow lotus.

10‧‧‧Light sterilizing tank

11‧‧‧ Groove

12‧‧‧Perforation

13‧‧‧Humidity control device

14‧‧‧ Temperature control device

15‧‧‧Humidity sensor

16‧‧‧Temperature sensor

20‧‧‧plant breeding equipment

21‧‧‧Conveyor

30‧‧‧Slice device

40‧‧‧First conveyor

41‧‧‧Main duct

42‧‧‧Management

50‧‧‧plant culture equipment

51‧‧‧ Petri dishes

60‧‧‧Second conveyor

70‧‧‧ callus screening device

80‧‧‧Central Processing Unit

Claims (5)

The invention relates to a snow lotus tissue culture culture device, which mainly comprises a light-transparent sterilization tank, a slicing device installed above the light-transmitting sterilization tank, and a plant breeding device connected to the slicing device by using the transport device, and is connected thereto. a slicing device and a first conveying device at the top of the transparent sterilization tank, a plant culture device installed at the bottom of the transparent sterilization tank, a callus screening device connected to the plant culture device by the second conveying device, and a central a processing unit, the central processing unit is electrically connected to the slicing device, the first conveying device, the second conveying device and the callus screening device respectively, thereby controlling the timing of each device; the snow lotus seedlings after the breeding is completed After being sliced by the slicing device, the first transport device can be transported to the plant culture device to cultivate the callus of the snow lotus, and the callus is transported to the callus screening device through the second transport device, and after the screening process, the cells are used. The way the group is collected to facilitate subsequent use. The snow lotus tissue culture culture device according to claim 1, wherein the bottom of the light-transmitting sterilization tank is formed with a plurality of grooves; the plant culture device comprises a plurality of petri dishes, each of which is accommodated in the culture dish. There is a medium which is mainly used in the process of tissue culture of Saussurea chinensis to induce the active ingredients of Xuelian. The squid culture culture device according to the second aspect of the invention, wherein the top of the transparent sterilization tank is formed with a plurality of perforations, wherein each perforation is vertically corresponding to the culture dish placed in the groove; The first conveying device comprises a main conveying pipe, and a plurality of manifolds communicating with the main conveying pipe, wherein the free end portion of each manifold is arranged in a corresponding perforation, thereby the young stem of the snow lotus The sections were evenly spread in each dish. The snow lotus tissue culture culture device according to claim 1, 2 or 3, wherein the light transmission sterilization tank is further provided with a humidity control device and a temperature control device, wherein the humidity control device and the temperature control device extend along the extension After entering the transparent sterilization tank, a humidity sensor and a temperature sensor are respectively disposed; the humidity control device and the temperature control device are respectively electrically connected to the central processing unit, thereby controlling the operation of the humidity control device and the temperature control device Time, in order to control the temperature and humidity in the transparent sterilization tank to the environment most suitable for cultivating the plant cells of the snow lotus. The snow lotus tissue culture culture device according to claim 4, wherein the humidity control device and the temperature control device are respectively connected to the plant breeding device, so as to control the temperature and humidity in the plant breeding device to be most suitable for the growth of the snow lotus seedlings. The environment.