CN100397976C - Method of cultivating selenium-rich pagodatree ear and method of extracting pagodatree ear selenium polysaccharide - Google Patents
Method of cultivating selenium-rich pagodatree ear and method of extracting pagodatree ear selenium polysaccharide Download PDFInfo
- Publication number
- CN100397976C CN100397976C CNB2006100459022A CN200610045902A CN100397976C CN 100397976 C CN100397976 C CN 100397976C CN B2006100459022 A CNB2006100459022 A CN B2006100459022A CN 200610045902 A CN200610045902 A CN 200610045902A CN 100397976 C CN100397976 C CN 100397976C
- Authority
- CN
- China
- Prior art keywords
- selenium
- pagodatree
- ear
- rich
- parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The present invention relates to a method of cultivating officinal fungi and application, particularly to a method of cultivating selenium-rich pagodatree ears and extracting selenium polysaccharide from pagodatree ears. The mother culture of the selenium-rich pagodatree ear is composed of potato, agar, glucose, soybean powder, magnesium sulfate, zinc sulfate, potassium dihydrogen phosphate, peptone, 6-BA, selenium-rich fertilizer, mulberry leaf or mulberry branch, and water; the stock culture of the selenium-rich pagodatree ear is composed of pagodatree wood dust, hard mixed wood dust, wheat bran, corn meal, soybean powder, gypsum, magnesium sulfate, zinc sulfate, 6-BA, brown sugar, lime, potassium dihydrogen phosphate, selenium-rich fertilizer and water. The stock culture, third-level strain and fungus bodies are made by means of inoculation, and then the polysaccharide is made from the fungus bodies. The present invention has the advantages that the ingredients of the culture medium create good environment for fungus polysaccharide for gathering the selenium; strong resistance performance is generated in the process of fungus growth and breeding of the pagodatree ear, which improves the yield and the quality of products largely. Through comparison experiments, the growth speed of the fungus of hyphae is 10 days earlier than that of the fungus in an ordinary medium; the pollution rate is lowered by 10 %, and the efficiency of cancer resistance is enhanced by more than 30%.
Description
Technical field
The present invention relates to the cultivation method and the application of medicinal fungi, specifically relate to the cultivation of selenium-rich pagodatree ear and the extracting method of pagodatree ear selenium polysaccharide.
Background technology
The Chinese scholartree ear is the excellent species in the good fungi of the anticancer effect known to the public, and its medical value is recorded in " Handbook of Prescriptions for Emergencies " of Ge Hong the earliest, and Chang Yiqi fruit body among the people is used as medicine, to treat multiple eqpidemic disease.Because the natural resources wretched insufficiency of Chinese scholartree ear, this causes very big breach for the huge market demand, and imbalance between supply and demand is quite outstanding.The artificial cultivation cycle is long, does not form scale as yet, and being difficult to provides required for market.Selenium also has very high medical value, and it is an indispensable element in the human body growth metabolism process, can promote immunologic function, the opposing virus of organism, the prevention variation.But selenium only is converted into biogenic organic selenium by inorganic, just has the assurance of absorption rate height, safety non-toxic, the good medical value of its antitumor grade of competence exertion.Yet do not find product in the market, and the report of selenium-rich pagodatree ear about the combination of bacterium selenium.Test shows: the anticancer effect of selenium-rich pagodatree ear is obvious more than common Chinese scholartree ear.
Summary of the invention
Purpose of the present invention provides a kind of cultivation of the selenium-rich pagodatree ear with anticancer function and the extracting method of pagodatree ear selenium polysaccharide.
The cultivation method of selenium-rich pagodatree ear is characterized in that this method may further comprise the steps:
1) the female preparation of planting of selenium-rich pagodatree ear; Described selenium-rich pagodatree ear is female plants by following compositions by making by weight ratio: 150～250 parts of potatos, 10～30 parts in agar, 10～30 parts of glucose, 5～15 parts of bean powderes, 1～3 part in magnesium sulfate, 0.5～1.5 part in zinc sulphate, 1～10 part of potassium dihydrogen phosphate, 1～10 part of peptone, 6-BA0.5～1.5 part, 1～5 part of rich selenium fertilizer, Folium sophorae or 50～150 parts of sophora flowers, 500～1500 parts in water;
Take by weighing each composition by above-mentioned weight portion proportioning, potato is cleaned section, Folium sophorae or sophora flower are cleaned, become phase-splitting to mix then with other, simmer in water after mixing and endure, treat to stop after the agar dissolving, through 8 layers of filtered through gauze, filtrate packing test tube, make mother culture media, medium is through autoclaving under 110～130 ℃, pressure 0.1～0.3Mpa condition 2 hours, aseptic inoculation Chinese scholartree ear tissue piece, unglazed cultivation is 7 days under 25 ℃ of constant temperatures, becomes selenium-rich pagodatree ear one godmother's kind;
Described Chinese scholartree ear tissue piece picks up from the wild Chinese scholartree ears or side handles of a utensil entity of beautiful Foshan, Anshan, Liaoning Province acacia woods.
Gained is female plants that mycelia is pure white, dense, sturdy, strong stress resistance, selenium polysaccharide content height.
2) preparation of selenium-rich pagodatree ear original seed; Described selenium-rich pagodatree ear original seed by following compositions by making by weight ratio: 30～40 parts of Chinese scholartree wood chips, 30～40 parts of hardwood crumbses, 10～20 parts in wheat bran, 2～10 parts of corn flours, 1～10 part of bean powder, 0.5～1.5 part in gypsum, 0.1～1 part in magnesium sulfate, 0.1～1 part in zinc sulphate, 6-BA0.05～0.15 part, 0.5～1.5 part in brown sugar, 0.5～1.5 part of lime, 0.1～1 part of potassium dihydrogen phosphate, 0.5～1.5 part of rich selenium fertilizer, 60～70 parts in water, pH value are 7;
By described weight portion proportioning, to bottle after the mentioned component mixing and stirring or pack, seal, under the condition of 120～135 ℃ of temperature, pressure 0.1～0.2Mpa, sterilized 2 hours, aseptic condition is implanted the female kind of selenium-rich pagodatree ear down, 25 ℃ of unglazed cultivation and fermentation of constant temperature 25～30 days, mycelia was covered with, and promptly gets original seed.
3) preparation of three grades of kinds; With step 2) pedigree seed culture medium inoculation original seed promptly make three grades of kinds;
4) preparation of sclerotinite; With step 2) three grades of kinds of pedigree seed culture medium inoculation promptly make selenium-rich pagodatree ear bacterium rod, i.e. sclerotinite or fruit body.
Described rich selenium fertilizer is meant one or more in sodium selenite, sodium selenate or the calcium selenate.
Described 6-BA is an auximone, all is applicable to this cultivating bacterial spawn through this series products of evidence.
Extract the method for pagodatree ear selenium polysaccharide with prepared selenium-rich pagodatree ear sclerotinite, it is characterized in that: selenium-rich pagodatree ear sclerotinite or fruit body powder are broken into the particle that particle diameter is 0.2-0.6mm, use water logging then, be after fully stirring under 70-80 ℃ the condition in temperature, filter, concentrate, drying promptly makes pagodatree ear selenium polysaccharide.
Advantage of the present invention is: the component of medium combines the sufficient condition that provides for polysaccharide with selenium, for fungi polysaccharide has been created good environment to the enrichment of selenium, cause sending out of Chinese scholartree ear and produce strong resistance in bacterium, the reproductive process, reduce the generation of disease, the output and the quality of product all are greatly improved, and through test contrast, mycelium is sent out bacterium speed than ordinary culture medium fast 5 days, antipollution power improves 10%, and its medicinal usefulness improves more than 20～30%.Its significant medical value of the fungi product of being cultivated in view of the above can further manifest in concrete practice, if it is played a role as a kind of Selenium supplement agent safely and effectively or healthcare products, will have more wide prospect.
Embodiment
Embodiment 1:
The cultivation method of selenium-rich pagodatree ear, this method comprises the steps:
1) the female preparation of planting of selenium-rich pagodatree ear; The female kind of selenium-rich pagodatree ear is to be made by weight ratio by following compositions: 200 parts of potatos, 20 parts in agar, 20 parts of glucose, 10 parts of bean powderes, 2 parts in magnesium sulfate, 1 part in zinc sulphate, 5 parts of potassium dihydrogen phosphates, 5 parts of peptones, 6-BA1 part, fertile 2 parts of rich selenium, Folium sophorae or spend 1000 parts of 100 parts, water;
Take by weighing each composition by above-mentioned weight portion proportioning, potato is cleaned section, Folium sophorae or sophora flower are cleaned, become phase-splitting to mix then with other, simmer in water after mixing and endure, treat to stop after the agar dissolving, through 8 layers of filtered through gauze, filtrate packing test tube, make mother culture media, medium is through autoclaving under 110～130 ℃, pressure 0.1～0.3Mpa condition 2 hours, aseptic inoculation Trametes robiniphila Murr piece of tissue, unglazed cultivation is 7 days under 25 ℃ of constant temperatures, becomes selenium-rich pagodatree ear bacterium one godmother's kind;
2) preparation of selenium-rich pagodatree ear original seed; The selenium-rich pagodatree ear original seed is to be made by weight ratio by following compositions: 35 parts of Chinese scholartree wood chips, 35 parts of hardwood crumbses, 15 parts in wheat bran, 6 parts of corn flours, 6 parts of bean powderes, 1 part in gypsum, 0.5 part in magnesium sulfate, 0.6 part in zinc sulphate, 0.1 part of 6-BA, 1 part in brown sugar, 1 part of lime, 0.5 part of potassium dihydrogen phosphate, fertile 1 part of rich selenium, 65 parts in water, pH value are 7;
By described weight portion proportioning, to bottle after the mentioned component mixing and stirring or pack, seal, under the condition of 120～135 ℃ of temperature, pressure 0.1～0.2Mpa, sterilized 2 hours, aseptic condition is implanted the female kind of selenium-rich pagodatree ear down, 25 ℃ of unglazed cultivation and fermentation of constant temperature 25～30 days, mycelia was covered with, and promptly gets original seed.
3) preparation of three grades of kinds; With step 2) pedigree seed culture medium inoculation original seed promptly make three grades of kinds;
4) preparation of sclerotinite; With step 2) three grades of kinds of pedigree seed culture medium inoculation promptly make selenium-rich pagodatree ear bacterium rod, i.e. sclerotinite or fruit body.
Described rich selenium fertilizer is meant one or more in sodium selenite, sodium selenate or the calcium selenate.
Extract the method for pagodatree ear selenium polysaccharide with prepared selenium-rich pagodatree ear sclerotinite: selenium-rich pagodatree ear sclerotinite or fruit body powder are broken into the particle that particle diameter is 0.2-0.6mm, use water logging then, be after fully stirring under 70-80 ℃ the condition in temperature, filter, concentrate, drying promptly makes pagodatree ear selenium polysaccharide.
Embodiment 2:
1) the female preparation of planting of selenium-rich pagodatree ear; The female kind of selenium-rich pagodatree ear is to be made by weight ratio by following compositions: 150 parts of potatos, 30 parts in agar, 30 parts of glucose, 5 parts of bean powderes, 1 part in magnesium sulfate, 0.5 part in zinc sulphate, 10 parts of potassium dihydrogen phosphates, 10 parts of peptones, 6-BA0.5 part, fertile 1 part of rich selenium, Folium sophorae or 50 parts of sophora flowers, 500 parts in water;
2) preparation of selenium-rich pagodatree ear original seed; The selenium-rich pagodatree ear original seed is to be made by weight ratio by following compositions: 30 parts of Chinese scholartree wood chips, 30 parts of hardwood crumbses, 10 parts in wheat bran, 10 parts of corn flours, 10 parts of bean powderes, 1.5 parts in gypsum, 1 part in magnesium sulfate, 1 part in zinc sulphate, 0.15 part of 6-BA, 1.5 parts in brown sugar, 1.5 parts of limes, 1 part of potassium dihydrogen phosphate, fertile 1.5 parts of rich selenium, 70 parts in water, pH value are 7;
The cultivation method step of selenium-rich pagodatree ear is with embodiment 1.
With the method for prepared selenium-rich pagodatree ear sclerotinite extraction pagodatree ear selenium polysaccharide, with embodiment 1.
Embodiment 3:
The female kind of selenium-rich pagodatree ear is to be made by weight ratio by following compositions: 250 parts of potatos, 10 parts in agar, 10 parts of glucose, 15 parts of bean powderes, 3 parts in magnesium sulfate, 1.5 parts in zinc sulphate, 1 part of potassium dihydrogen phosphate, 1 part of peptone, 6-BA1.5 part, fertile 5 parts of rich selenium, Folium sophorae or spend 1500 parts of 150 parts, water;
The selenium-rich pagodatree ear original seed is to be made by weight ratio by following compositions: 40 parts of Chinese scholartree wood chips, 40 parts of hardwood crumbses, 20 parts in wheat bran, 2 parts of corn flours, 1 part of bean powder, 0.5 part in gypsum, 0.1 part in magnesium sulfate, 0.1 part in zinc sulphate, 6-BA0.05 part, 0.5 part in brown sugar, 0.5 part of lime, 0.1 part of potassium dihydrogen phosphate, fertile 0.5 part of rich selenium, 60 parts in water, pH value are 7;
The cultivation method step of selenium-rich pagodatree ear is with embodiment 1.
With the method for prepared selenium-rich pagodatree ear sclerotinite extraction pagodatree ear selenium polysaccharide, with embodiment 1.
Claims (3)
1. the cultivation method of selenium-rich pagodatree ear is characterized in that this method may further comprise the steps:
1) the female preparation of planting of selenium-rich pagodatree ear; Described selenium-rich pagodatree ear is female plants by following compositions by making by weight ratio: 150～250 parts of potatos, 10～30 parts in agar, 10～30 parts of glucose, 5～15 parts of bean powderes, 1～3 part in magnesium sulfate, 0.5～1.5 part in zinc sulphate, 1～10 part of potassium dihydrogen phosphate, 1～10 part of peptone, 6-BA0.5～1.5 part, 1～5 part of rich selenium fertilizer, Folium sophorae or 50～150 parts of sophora flowers, 500～1500 parts in water;
Take by weighing each composition by above-mentioned weight portion proportioning, potato is cleaned section, Folium sophorae or sophora flower are cleaned, become phase-splitting to mix then with other, simmer in water after mixing and endure, treat to stop after the agar dissolving, through 8 layers of filtered through gauze, filtrate packing test tube, make mother culture media, medium is through autoclaving under 110～130 ℃, pressure 0.1～0.3Mpa condition 2 hours, aseptic inoculation Chinese scholartree ear tissue piece, unglazed cultivation is 7 days under 25 ℃ of constant temperatures, becomes selenium-rich pagodatree ear one godmother's kind;
2) preparation of selenium-rich pagodatree ear original seed; Described selenium-rich pagodatree ear original seed by following compositions by making by weight ratio: 30～40 parts of Chinese scholartree wood chips, 30～40 parts of hardwood crumbses, 10～20 parts in wheat bran, 2～10 parts of corn flours, 1～10 part of bean powder, 0.5～1.5 part in gypsum, 0.1～1 part in magnesium sulfate, 0.1～1 part in zinc sulphate, 6-BA0.05～0.15 part, 0.5～1.5 part in brown sugar, 0.5～1.5 part of lime, 0.1～1 part of potassium dihydrogen phosphate, 0.5～1.5 part of rich selenium fertilizer, 60～70 parts in water, pH value are 7;
By described weight portion proportioning, to bottle after the mentioned component mixing and stirring or pack, seal, under the condition of 120～135 ℃ of temperature, pressure 0.1～0.2Mpa, sterilized 2 hours, aseptic condition is implanted the female kind of selenium-rich pagodatree ear down, 25 ℃ of unglazed cultivation and fermentation of constant temperature 25～30 days, mycelia was covered with, and promptly gets original seed;
3) preparation of three grades of kinds; With step 2) pedigree seed culture medium inoculation original seed promptly make three grades of kinds;
4) preparation of sclerotinite; With step 2) three grades of kinds of pedigree seed culture medium inoculation promptly make selenium-rich pagodatree ear bacterium rod, i.e. sclerotinite or fruit body.
2. the cultivation method of selenium-rich pagodatree ear according to claim 1 is characterized in that described rich selenium fertilizer is meant one or more in sodium selenite, sodium selenate or the calcium selenate.
3. extract the method for pagodatree ear selenium polysaccharide with claim 1 or 2 prepared selenium-rich pagodatree ear sclerotinites, it is characterized in that: selenium-rich pagodatree ear sclerotinite or fruit body powder are broken into the particle that particle diameter is 0.2-0.6mm, use water logging then, be after fully stirring under 70-80 ℃ the condition in temperature, filter, concentrate, drying promptly makes pagodatree ear selenium polysaccharide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100459022A CN100397976C (en) | 2006-02-24 | 2006-02-24 | Method of cultivating selenium-rich pagodatree ear and method of extracting pagodatree ear selenium polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100459022A CN100397976C (en) | 2006-02-24 | 2006-02-24 | Method of cultivating selenium-rich pagodatree ear and method of extracting pagodatree ear selenium polysaccharide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1810082A CN1810082A (en) | 2006-08-02 |
| CN100397976C true CN100397976C (en) | 2008-07-02 |
Family
ID=36843316
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2006100459022A Expired - Fee Related CN100397976C (en) | 2006-02-24 | 2006-02-24 | Method of cultivating selenium-rich pagodatree ear and method of extracting pagodatree ear selenium polysaccharide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100397976C (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101525646B (en) * | 2009-03-06 | 2011-11-09 | 苏源 | Pnoliota adiposa mycelium selenium enrichment cultivation and technology for extracting pnoliota adiposa selenium polysaccharide |
| CN103493687B (en) * | 2013-10-17 | 2015-04-01 | 王广前 | Trametes robinioplila cultivating method |
| CN103688758B (en) * | 2013-12-15 | 2016-03-16 | 张爱美 | A kind of original ecology cultivating method of Chinese scholartree ear |
| CN108370839A (en) * | 2018-01-25 | 2018-08-07 | 桂林灌阳桂灌菌业科技开发有限公司 | A kind of Wild ganoderma mother culture media and preparation method thereof |
| CN115316196B (en) * | 2022-08-16 | 2023-09-15 | 哈尔滨市农业科学院 | Mutagenesis and cultivation method of wild locust Fan Shikong bacteria, locust Fan Shikong bacteria and application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1205833A (en) * | 1997-07-21 | 1999-01-27 | 甄兆玄 | Cultivation process of edible locust tree fungus |
| CN1233389A (en) * | 1998-04-28 | 1999-11-03 | 山东省滨州卫生学校 | Method for cultivating selenium-rich glossy ganoderma |
-
2006
- 2006-02-24 CN CNB2006100459022A patent/CN100397976C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1205833A (en) * | 1997-07-21 | 1999-01-27 | 甄兆玄 | Cultivation process of edible locust tree fungus |
| CN1233389A (en) * | 1998-04-28 | 1999-11-03 | 山东省滨州卫生学校 | Method for cultivating selenium-rich glossy ganoderma |
Non-Patent Citations (8)
| Title |
|---|
| 增强食用菌对硒生物富集作用的探讨. 潘慧.科技创业月刊，第8期. 2004 |
| 增强食用菌对硒生物富集作用的探讨. 潘慧.科技创业月刊，第8期. 2004 * |
| 富硒食用菌的栽培技术. 黎香兰等.北京农业，第8期. 2003 |
| 富硒食用菌的栽培技术. 黎香兰等.北京农业，第8期. 2003 * |
| 食用菌市场新宠－－富硒蘑菇. 曹德宾.农村实用技术，第10期. 2005 |
| 食用菌市场新宠－－富硒蘑菇. 曹德宾.农村实用技术，第10期. 2005 * |
| 黑木耳富硒栽培研究. 梁英等.食用菌学报，第7卷第4期. 2000 |
| 黑木耳富硒栽培研究. 梁英等.食用菌学报，第7卷第4期. 2000 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1810082A (en) | 2006-08-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100399877C (en) | Cultivation of selenium enriched morin and method of extracting morin selenium polysacharide | |
| CN104030845A (en) | Lucid ganoderma substitute cultivation medium with momordica grosvenori | |
| CN101317526A (en) | Production and cultivation of glossy ganoderma with tea branch, tea, stem material | |
| CN102786333A (en) | Phellinus igniarius bag cultivation medium and method for cultivating phellinus igniarius sporophore by same | |
| CN103027293A (en) | Method for preparing antrodia camphorata ferment | |
| CN104946541A (en) | High-density liquid strain fermentation medium | |
| CN100397976C (en) | Method of cultivating selenium-rich pagodatree ear and method of extracting pagodatree ear selenium polysaccharide | |
| CN104030844A (en) | Lucid ganoderma substitute cultivation medium with common andrographis herb | |
| CN107652048A (en) | A kind of cultivation matrix of mushroom enhancing immunization and preparation method thereof | |
| CN102165894A (en) | Method for carrying out rich-selenium culturing of plelmtus eqngiu by utilizing sodium selenite | |
| CN104844354A (en) | High-density pleurotus eryngii liquid strain fermentation medium | |
| CN104988070A (en) | Pleurotus eryngii liquid strain with small and many mycelium pellets and fermentation method of pleurotus eryngii liquid strain | |
| CN102870594B (en) | Method for culturing hericium erinaceus through tobacco stem facility | |
| CN108901607A (en) | It is a kind of to have effects that the method for cultivating mushroom of benefiting qi and nourishing blood | |
| CN104030848A (en) | Lucid ganoderma substitute cultivation medium with trollflower | |
| CN104982220A (en) | Liquid spawn and fermentation method thereof | |
| CN108293593B (en) | Method for cultivating black fungus by using corncob mixture | |
| CN107873391B (en) | Fungus inhibiting culture medium for cultivating agaric and cultivation method thereof | |
| CN108586102A (en) | A kind of Se-rich lucid ganoderma culture medium and preparation method thereof | |
| CN104756767A (en) | High-density pleurotus eryngii liquid strain and fermentation method thereof | |
| CN108002877B (en) | Culture medium for cultivating oyster mushroom by using moxa dregs and cultivation method thereof | |
| KR100752335B1 (en) | Method for culturing sangwhangletari mushroom using phellinus linteus mycelium and letari mushroom, and the mushroom culture mat for sangwhangletari mushroom | |
| CN102850118B (en) | Black fungus culture base and preparation method thereof | |
| CN109197375A (en) | One kind having health care tea tree mushroom cultivation method | |
| CN100469868C (en) | Method of cultivating bacterial spawn for umbellate pore fungus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: LIAONING DONGFANG BIOLOGY TECHNOLOGY INSTITUTE Free format text: FORMER OWNER: CUI PENG Effective date: 20100930 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 114000 NO.29, BUILDING 52, HUNANDASHI STREET, TIEDONG DISTRICT, ANSHAN CITY, LIAONING PROVINCE TO: 114000 WEIJIATUN, HIGH-TECH ZONE, ANSHAN CITY, LIAONING PROVINCE |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20100930 Address after: 114000 Liaoning city of Anshan province high tech Zone Wei Jia Tun Patentee after: Liaoning Dongfang Biotechnology Research Institute Address before: 114000, No. 52, No. 29, Dashi street, MTR East, Anshan, Liaoning, Hunan Patentee before: Cui Peng |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080702 Termination date: 20180224 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |